LOCUS       DASSFD010000001         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_381394, whole genome shotgun sequence.
ACCESSION   DASSFD010000001 DASSFD010000000
VERSION     DASSFD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381394"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..544)
                     /locus_tag="VFH39_00005"
     CDS             complement(251..544)
                     /locus_tag="VFH39_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924198.1"
                     /translation="MTNANINIEEATKLQAILDNLPPEAYEPGLPRRSFLGFKDGQPV
                     GAYMVALREAESEAARGVHRTERRCIEFGLALSGLSAAAIGNVMAQAAQSELQ"
     gene            complement(607..1911)
                     /gene="serS"
                     /locus_tag="VFH39_00010"
     CDS             complement(607..1911)
                     /gene="serS"
                     /locus_tag="VFH39_00010"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6924199.1"
                     /translation="MLDIQFIRDNRDLVAEKSRQKGYDVDIPQLLELDGERRSKQTQI
                     DELRQRRNSLAAEFKNQKPSPEQLEQGRQLKEELGQREEELAHVEDKYLALLKKVPNM
                     PLADVPVGASEDENVVAKTVGEPTQFDFAPKSHADIAEAKGWLDKERAAKVTGSRFAY
                     IKGDLVRLQFAIVQFVITKLTDPNFIDEVIVENGLTNVSNKPFVPVLPPLMLRESLYD
                     AMDRLEPRDDRYKLEGDDDLWLQGSAEHVLGSMHADEILAESDLPLRYLGYATSFRRE
                     AGTYGKDMEGMFRMHQFDKLEMESLTLPENGLQEHFLLVGIQEKLMQLLGIPYHVLTK
                     CTADIGKPNARGVDIEAWLPHQAKYRETHTADYMTDYQARRLKTRVKRADGSVELVHT
                     NDATAFALSRTPIAIIENFQTPEGNVRVPEVLQPFLNGQSEL"
     gene            complement(1916..2683)
                     /locus_tag="VFH39_00015"
     CDS             complement(1916..2683)
                     /locus_tag="VFH39_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6924200.1"
                     /translation="MPSHAQVELIRVDDPDAPEDQYLLAQGHMAERRLGSLALLYLHG
                     WRRPSNDRAARRTAEVGWDSYVVNFRGHNGSDGDIRKVSRADALLDALSAYDYIEEQQ
                     PPGTRIGLVTHSFSAYIGILLTQHRDVACISMRAGANYADKGFDRPQYGKGHVDPAVM
                     RFREQKLRWNENKALTAIHEYGGPIQIIESGQDTYVPHQTTQNYLDAASDPSKVEYHL
                     KPEWEHLLVGNDRCLTEYEDILIGWLRNSVEPALRLQ"
     gene            2805..>3197
                     /locus_tag="VFH39_00020"
     CDS             2805..>3197
                     /locus_tag="VFH39_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924201.1"
                     /translation="MQKTESPAPTAPLDKKARRIRPLDVAIVVLFVAVVTALILVLLA
                     QLRLKNEVADARAVTDKLITAMKDQDGEAARLLGDPGFRARNSADQMHTIFTESKPYT
                     QGTPVVVRQVVVNGKVNHVVSIYYKFGSQ"
BASE COUNT          575 a          946 c          923 g          753 t
ORIGIN      
        1 ttcagttcga ccacgacgtg cacgctatca cgatgatgtt tcggcagata gcggtcgagg
       61 cggcctatct tgcgggtgac gtatttacgt aagttatcat cgaccttggc gtggacacct
      121 ttgatctcaa acttctctat catggccgcg gcctcctttc gatgttaacg acgcgtcaga
      181 gcaacttcgt actctcatta tacgccccgg cgccaagccg tctgtgaggc cggtcaatgc
      241 tttttagggc ctactgcagc tcgctctgcg cggcttgcgc catgacgttg ccgatagctg
      301 cagcggataa accactcagt gcgaggccga attcgatgca acgccgttcg gtacggtgga
      361 cgccgcgggc cgcttctgac tcggcttcgc gcaaggctac catgtatgcg ccgaccggct
      421 ggccgtcctt aaagcctaag aacgatcgcc gcgggagacc cggttcgtac gcctccggtg
      481 gtaagttatc taagatagcc tgtaattttg tcgcttcctc aatatttatg ttagcgttgg
      541 tcacgtcttc gctccgttaa ctatgactta atcataagcc tgttcgcgtg gctgtcaatg
      601 gagcggctag agttcgctct ggccgttcag gaacggctgc agcacttcgg ggacacgcac
      661 attgccctca ggcgtctgga agttttcgat gatggcgatg ggcgtccggc tgagcgcgaa
      721 ggcggtggcg tcgttggtgt gcaccagttc gacgctgccg tcggcccgct tcacccgggt
      781 tttgagccgg cgcgcctgat agtcggtcat gtagtcggcc gtgtgggtct cgcggtattt
      841 ggcctggtgc ggcaaccagg cttcgatgtc cacgccccgg gcgttcggct tgccgatatc
      901 ggccgtgcac ttggtgagca cgtggtacgg gatgcccagc agctgcatga gtttttcctg
      961 gataccgacc agcaggaagt gctcctgcag gccgttttcg ggcaatgtca ggctttccat
     1021 ttccagcttg tcgaactggt gcatacggaa catgccttcc atgtctttgc cgtaggtgcc
     1081 ggcttcgcgc cggaaactgg tggcgtaccc caggtagcgt agcggcagat cggattcggc
     1141 caggatttcg tcggcatgca tgctgcccag cacgtgctcg gcactgccct gcagccacag
     1201 gtcgtcgtcg ccctccagct tgtagcggtc gtcacgcggt tccagccggt ccatggcgtc
     1261 gtacagcgat tcgcgcagca tcagcggcgg caacaccggc acgaacggct tgttgctgac
     1321 attcgtcaaa ccgttttcga caattacttc atctataaaa ttggggtctg tcagtttggt
     1381 aatgacaaac tgcacgatgg cgaactgcag ccgcaccaga tcacctttta tgtacgcgaa
     1441 gcggctgccg gtgactttgg cggcacgctc tttgtcgagc cagcctttgg cttcggcgat
     1501 atcggcatga ctcttgggcg caaagtcgaa ctgcgtcggt tcgccgacgg ttttggcgac
     1561 gacgttctcg tcctcgctgg cgcccaccgg cacgtctgcg agcggcatgt tcggcacttt
     1621 tttgagcagc gccagatact tgtcttctac atgcgccagc tcttcttcgc gctggccgag
     1681 ttcctccttc agctgccgtc cctgctccag ctgttccggc gaaggtttct ggttcttgaa
     1741 ttcggccgcc aggctgttgc ggcgctggcg cagctcgtcg atctgggtct gcttgctgcg
     1801 gcgctcgccg tcgagctcca gcagctgcgg aatatcgacg tcataaccct tttggcggga
     1861 tttttcggcc acgaggtcac ggttgtcacg gatgaactgg atatctagca tgcgtctatt
     1921 gtaaccgcaa cgccggctct acgctattgc gcagccagcc aatcaatata tcctcgtact
     1981 cggtcaggca gcgatcgttg cccaccagca agtgctccca ctcaggtttc agatggtatt
     2041 cgacttttga ggggtcggac gcggcatcga gataattctg cgttgtctga tggggcacgt
     2101 aggtatcctg gccggattcg attatctgaa tcggtccccc atattcgtgg atagccgtca
     2161 gtgccttgtt ttcgttccaa cgcaatttct gttcgcggaa tctcatgaca gccggatcga
     2221 cgtgtccttt gccgtactgc ggcctgtcga aacccttgtc ggcgtagttc gccccggccc
     2281 gcatcgatat gcaggccacg tcccgatgct gggtaagcag aatgcctatg taagccgaga
     2341 agctatgggt aaccaagccg atacgggtac ccggaggctg ctgttcctcg atgtaatcgt
     2401 aagccgacag cgcatcgagc agcgcatcgg cgcggctgac cttgcggata tctccgtcgc
     2461 tgccgttgtg gccccggaaa ttgacgacat acgagtccca acccacctcg gccgtgcgac
     2521 gcgctgcccg gtcgttcgag gggcgacgcc agccgtgcaa gtacagcaat gccagtgaac
     2581 cgagccgccg ttcggccata tgaccttgtg ccagtagata ttggtcctcg ggcgcatccg
     2641 ggtcatccac cctgataagt tcaacctgtg cgtgtgatgg catgcggatt agtatactgc
     2701 agccagtcaa gctgtctgag atattcatca cgttccgatg gctgacgacc tggctgtaag
     2761 aattctcatt cagccgcttc gcctttttcg cgtataaatc tggtatgcag aagaccgaat
     2821 cgcctgctcc gaccgcgccc ctggataaaa aagcgcggcg catccgcccg ctcgatgtag
     2881 cgattgtggt gctgttcgta gcggtcgtga cggctttgat tctcgtgcta ttggcccagc
     2941 tacggctcaa gaatgaagtc gccgatgccc gcgcggttac cgacaaactc attactgcca
     3001 tgaaggatca ggacggcgaa gccgcccggc tgctcggcga tcccggcttc cgggcccgca
     3061 acagcgccga ccagatgcat accatcttca ccgagagcaa accctatacc caagggacgc
     3121 cggtcgtcgt ccgccaggtc gtggtcaacg gcaaggtcaa ccacgtggtg agcatctact
     3181 acaaattcgg cagccaa
//
LOCUS       DASSFD010000002         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1398147, whole genome shotgun sequence.
ACCESSION   DASSFD010000002 DASSFD010000000
VERSION     DASSFD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398147"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VFH39_00025"
     CDS             complement(<1..994)
                     /locus_tag="VFH39_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET6924202.1"
                     /translation="MAKRFIYMDYAAATPMDLAVVAAMQPYFSERFYNPSALYAAGLE
                     ARADVEKARAAVAHRFGARPSEIVFTAGATEANNLAIHGVMRRYPQANLVVSAVEHDS
                     VLAPAGQYGRLLAPVHADGLVDTAALTKLIDDHTVLVSVQYANNEVGTIQPLKEVSRS
                     VRSIRMERQRAGNKLPLYLHTDAAQAAGYLELHAMRLGADLISINGGKIYGPKQSGAL
                     YVGAGIVLSPLIHGGGQERGLRSGTENVAGCIGLATALDLVQSDRRTQAVRMRALQEL
                     FLQELGEKVPAATVNGSRKHRLPNNIHVTIPGQDNERLLFGLDEAGVQAATGSAC"
     gene            complement(1014..1373)
                     /locus_tag="VFH39_00030"
     CDS             complement(1014..1373)
                     /locus_tag="VFH39_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924203.1"
                     /translation="MKFLPKILSQNSEERRTETYRQLMRREAKKGGRLFGPVPAGHRR
                     EFFCLDEHTWVWHEEWTDETGRHQAVTTRYDIRPSGVLKSQGTNFYQEVTGDELRHLY
                     RAAKLFRAQLAAELQAL"
     gene            complement(1373..1717)
                     /locus_tag="VFH39_00035"
     CDS             complement(1373..1717)
                     /locus_tag="VFH39_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924204.1"
                     /translation="MAKILPFPGNNPEKPVSPEEKQLLAVYFSVVDESFMKRLRVVAW
                     QKDPAMIRQEDGLPSPADIPQKLPSARLSYDASQATNNVIYLANRQEEQAFTEQQRLA
                     EEALRRARREAV"
     gene            complement(1722..>2320)
                     /locus_tag="VFH39_00040"
     CDS             complement(1722..>2320)
                     /locus_tag="VFH39_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6924205.1"
                     /translation="VGIHPHEAQHYLSKTAQSQFASLLEDEPSDKKIVAVGECGLDFY
                     YNHSPKEVQIEMLKFQLELAKKHDLPVIFHVREAFADFWPLYDRYRVRGVLHSFTDSM
                     ENLQKALERGLFIGVNGIATFAKGADRLEMYKAIPESNLLLETDAPYLTPTPYRGTVN
                     EPKHIPEIAAYMAQLQGVAMEELAAATTQNAVSLFNLK"
BASE COUNT          466 a          701 c          642 g          511 t
ORIGIN      
        1 aacaggccga accggtcgcc gcctgcacgc ccgcttcgtc cagcccgaac agcaggcgct
       61 cattgtcctg gccgggtata gtaacgtgta tgttattggg cagacggtgt ttacggctgc
      121 cattcacggt agccgccggc actttctctc ccagttcttg caggaagagt tcctgcaagg
      181 cccgcatacg caccgcctga gtccgacgat cggactgcac gaggtccaga gcggtcgcca
      241 ggccgatgca gccggccacg ttttcggtgc cgctgcgcaa tccccgctcc tgaccaccgc
      301 cgtgaatcag cggcgagagc acgatgccgg caccgacata cagcgcgccg ctctgcttcg
      361 gaccgtatat cttgccgcca ttgatactta taagatcagc gcccagacgc atggcgtgca
      421 gctccaggta gccggctgcc tgggccgcat cggtgtgcag gtacagcggc agcttgttgc
      481 cggcccgttg gcgctccata cggatagaac gcaccgatcg gctgacttct ttaaggggct
      541 gaatagtacc gacttcattg ttggcgtatt gcacgcttac cagcaccgtg tggtcatcta
      601 tcagcttggt cagtgctgcg gtatcaacaa gcccgtcagc gtgtaccggt gccaaaaggc
      661 ggccgtactg cccggccggt gccagtaccg aatcgtgctc tacggcgctc accaccagat
      721 tggcctgcgg atagcgccgc ataacaccgt gtatggccag gttgttggct tcggtagcac
      781 cggccgtaaa aacgatttcg ctcggccggg cgccgaaccg gtgcgccact gccgcccggg
      841 ccttttccac atccgcgcgc gcttccaacc ctgccgcata caacgccgaa gggttataaa
      901 accgttcgct gaagtagggc tgcatggccg ccactaccgc cagatccatc ggtgtcgccg
      961 cggcataatc catataaatg aaacgcttcg ccataggtaa gattcagtat aaactagaga
     1021 gcttgcagtt cggcggccag ctgggcacgg aacagtttgg cggcccggta caggtggcgc
     1081 agttcatcgc cggtaacttc ttggtagaag ttagtcccct gcgacttcag cacgccgctc
     1141 ggacggatat cgtagcgggt ggtaaccgcc tggtgccggc ccgtttcatc ggtccattcc
     1201 tcgtgccaca cccaggtgtg ttcatccagg cagaaaaatt cacggcgatg tccagcgggc
     1261 accggcccga acagccgccc gcccttcttg gcctcccggc gcatgagctg ccggtacgtc
     1321 tcggtacgcc gctcctccga attctgggac agtatcttgg gcaggaattt cattacaccg
     1381 cctcccgccg ggcacggcgc aaagcttctt ccgccaggcg ctgttgttcc gtaaaggcct
     1441 gttcctcctg gcggttagcc agataaatca cgttgttggt cgcctgggag gcgtcgtagg
     1501 aaagccgggc ggaaggcagc ttctgcggta tgtccgcggg tgaaggcaaa ccgtcttcct
     1561 gacggatcat agcagggtcc ttttgccagg caacaacccg cagacgtttc ataaaactct
     1621 catccaccac cgaaaagtaa acagccagca gctgcttctc ttcgggagaa accggctttt
     1681 cggggttatt accgggaaac gggagtattt tggccattgc tttactttaa attaaaaaga
     1741 ctaacggcgt tttgcgtggt agcagccgcc agttcctcca ttgccacccc ttgtaattgc
     1801 gccatgtaag ctgcaatttc gggtatatgt ttcggctcgt ttactgtacc acgataaggg
     1861 gtgggagtca agtacggcgc gtctgtttca agcagcaaat tgctttccgg aatggctttg
     1921 tacatttcca gccggtctgc gcctttagcg aaggtagcga taccattcac ccctataaac
     1981 agaccgcgtt ccagcgcttt ttgcaaattc tccatgctgt ccgtgaaact atgcagtacc
     2041 ccgcgcaccc ggtagcggtc gtacaagggc cagaaatcgg caaaagcctc gcggacatgg
     2101 aatatcaccg gcaggtcatg ctttttggcc agctcaagct ggaacttcag catctcgatc
     2161 tgcacttctt tcggcgagtg attatagtaa aaatccaacc cgcactcccc caccgccaca
     2221 atcttcttat ccgaaggctc gtcctctaac agagaggcga attgtgattg tgcagtttta
     2281 ctgaggtagt gctgcgcttc gtgcgggtga atgcctacgg
//
LOCUS       DASSFD010000003         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1396069, whole genome shotgun sequence.
ACCESSION   DASSFD010000003 DASSFD010000000
VERSION     DASSFD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396069"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VFH39_00045"
     CDS             complement(<1..108)
                     /locus_tag="VFH39_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET6924206.1"
                     /translation="MRILGIDPGTGILGFGVIETNRGKPQLVDGGVIRTP"
     gene            136..1191
                     /locus_tag="VFH39_00050"
     CDS             136..1191
                     /locus_tag="VFH39_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET6924207.1"
                     /translation="MNFTFYSKTDYLTQLAQDIDHAKPGERVLVATMDFNSGEPLIAE
                     VLESLLRAAERGVKASLLVDARSFLTVDNSLKPGPLWWHPTLGQPFEGRYKTTMDWVA
                     RLNEAGCRTVVTNVPDRAFRFPSASRSHIKAGIIADKLYLGGCNLNHQEYFDVMVAQH
                     NKEAADWVYEQLQLVLDAKSTQQAFQYKDRSATIGNDTSVLIDSGKPKQSIILDAAHT
                     LIDEAQEWLYITCQYFPGGATAKRLVAAERRGVKVTIRFSHPSVHGQEFPLHVLHQLY
                     ERTRVPAHFFAGRLAKGMPKLHAKVLCSEKEAIVGSHNFVSQGVTWGTAEIALHVKNQ
                     DFARGLREFMEAQINRQ"
     gene            complement(1178..1666)
                     /locus_tag="VFH39_00055"
     CDS             complement(1178..1666)
                     /locus_tag="VFH39_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6924208.1"
                     /translation="MAGKTVICKDVHGKEYEVQVSQLRWRPSAYGIVIRAGKILLSKQ
                     FDSYDLPGGGVELGETPEEAVVREVKEETGMEVRSRELLTTRTSFFTWPDPEAPEHNE
                     ALMFYYACELVGGELSMDGFDEDEKIYADMPEWVPLEKLDSFKPASTVDWRPIVRQAI
                     AD"
     gene            complement(1693..2418)
                     /locus_tag="VFH39_00060"
     CDS             complement(1693..2418)
                     /locus_tag="VFH39_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6924209.1"
                     /translation="MSGHSKWSTIKREKGAKDAARGAVFTKLGNAIAIAARSGTDPET
                     NFALRLAIDKAKAANMPSANVQRAIDRIKDKNAAQLQEVLYEGYGPGGVAILVECATD
                     NINRTYPEVKLAFSKHGGSIAEKGSVAFQFEHKGSIRVKGTGDDIMLQAIEAGAEDVQ
                     EEGDESVIYTANTDLAKVRDALKDTGLEITEAELTYVPTNTIEITDKDTAGKIMRLMD
                     ALDDIDDVVNTHVNFDIDDALLQ"
     gene            complement(2479..>3782)
                     /locus_tag="VFH39_00065"
     CDS             complement(2479..>3782)
                     /locus_tag="VFH39_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET6924210.1"
                     /translation="VSLGWWRGSQYMLKMAEVGQYYDRKVTITAIAKQDAAYNKYGQL
                     AFDASDVTVVATGDKLAGKYSLSGFGYNAVFAGDEVTATGKLKSSLGSYQGRMSYAQV
                     EVSAHHNSLVNELRRRFTAGMQSALPEPLASFGMGLLIGQRNNLPAAVSQALLMVGLT
                     HIIAVSGYNLTILLRSAERVSGRRSKRLTTLLAFGLIAVFLLFAGNSASIVRAAIVSM
                     LSIAAGYSGRQFKPLVLLLLAAAITGFASPFYVWTDTSWYLSFLAFYGVMVVAPLVSR
                     NWRPRWRESLVVMVALESLCAEVMTLPFILHTFGQMSFIGIVANVVVAALVPLAMLLS
                     LIAGLAGMLLPALVGWLAWPAAIVLIYMLDMVQLLSRIPHVFAQNLGFSTAYMILSYA
                     VIVFLTAVVWWKKKPDNAKITEKASAAAPLAGTALSSTANA"
BASE COUNT          827 a         1088 c         1035 g          832 t
ORIGIN      
        1 aggggtgcgt atgacaccgc cgtcgactag ctgcggcttg ccgcggttgg tttcaatcac
       61 gccgaagccc aaaataccgg tgcctgggtc gattccaagt atgcgcatgc tgcgtatagt
      121 ataaaatgaa gctgcatgaa cttcacgttt tacagcaaga ccgactatct tacacaactc
      181 gcgcaggaca tcgaccacgc caaaccaggc gagcgcgtac tggtagctac tatggatttc
      241 aacagcgggg agcccttgat tgccgaggtg cttgagtcgt tgctacgagc agctgaaagg
      301 ggcgtgaagg cctccctttt ggtagatgcc cgttcattcc ttacggtcga taactcactt
      361 aagcctggcc cgctgtggtg gcaccccaca ttgggccagc catttgaagg gcgatacaaa
      421 acaaccatgg actgggtggc gcgcctgaac gaggcaggct gccgcacggt ggttaccaat
      481 gtacccgacc gcgcgttccg tttcccttcg gccagccgat cacacatcaa ggcaggcata
      541 atagccgata agttgtacct gggtggctgc aatctgaacc atcaggaata tttcgatgtc
      601 atggttgccc agcacaataa agaagcggcg gactgggttt acgaacagtt gcagttggtg
      661 ctggatgcca aaagcacaca gcaggcgttt cagtataagg atcgttcagc gaccataggt
      721 aatgacacct cggtgctaat agactccggt aagccaaagc aatcgataat tctcgatgcc
      781 gcccatacgc tgattgatga agcccaagag tggctgtaca taacctgcca gtattttcct
      841 ggtggggcga ctgccaagcg gttggtcgcc gccgaacgcc gtggcgtaaa ggtaacgatc
      901 cgcttcagcc acccgtcggt gcatggccag gaatttccgc tccacgtatt gcaccagctc
      961 tatgagcgca cgcgcgtgcc ggcgcatttt tttgcgggca ggctggcgaa aggcatgccc
     1021 aaactgcacg ccaaggtgtt atgctccgag aaggaagcga tcgtaggcag ccataatttc
     1081 gtctcacaag gcgtcacgtg gggcaccgcc gagattgctt tacatgttaa gaatcaggat
     1141 tttgcccgcg gcctgcgcga gtttatggaa gcgcaaatca atcggcaata gcttgccgta
     1201 caatcggccg ccaatcgact gttgaggctg gcttgaaact atctagtttt tccagaggaa
     1261 cccattccgg catgtcggca taaatctttt cgtcctcgtc aaaaccgtcc atcgacagtt
     1321 caccgccaac cagttcgcaa gcgtaataaa acatgagtgc ttcattgtgc tcaggtgctt
     1381 cggggtccgg ccaggtaaag aaactggtcc gcgtcgtcag aagttcccgc gatctgactt
     1441 ccatgccggt ttcctctttg acctcgcgga caactgcctc ttccggggtc tcgcctaact
     1501 ccacgcctcc acccggcaag tcgtaactgt cgaattgctt ggaaagcaga atcttgccgg
     1561 cgcgtatgac gataccatac gcagagggac gccaccggag ctgtgacacc tgcacttcgt
     1621 attctttgcc gtgaacatct ttgcaaatca ccgttttccc ggccatcgca gattcctttg
     1681 tttacgatac ggctattgta gcagggcgtc gtcgatgtcg aaattgacgt gggtgtttac
     1741 gacgtcgtcg atgtcgtcca gggcgtccat gaggcgcatg atcttgccgg cggtgtcttt
     1801 gtcggtaatc tcgatggtgt tggtcggcac atacgtaagc tcggcttcgg taatctccag
     1861 cccagtgtcc ttgagggcat cacgcacttt ggccaggtcg gtgttggcgg tgtagatgac
     1921 gctttcgtcg ccttcttcct gcacatcctc ggcgccggcc tcgatggcct gcagcataat
     1981 gtcatcgccc gtgcctttga cccggatact gcctttgtgc tcgaactgga aggccactga
     2041 gcccttctcg gcaatgctgc cgccgtgctt gctgaaagcc agctttactt ctgggtaggt
     2101 acgattgatg ttgtcggtgg cgcactccac cagaatggcg acgccgccgg gcccatagcc
     2161 ttcatacagc acttcctgca gctgcgcggc gttcttgtcc ttaatgcgat cgatggcgcg
     2221 ctggacatta gcgctgggca tattggctgc cttggcctta tctatggcca gacgcaaggc
     2281 gaagttggtc tccgggtcgg tgccgctgcg cgcagcgata gcgatggcgt tgccgagctt
     2341 ggtgaacacg gcaccgcgag ccgcgtcctt ggcgcctttc tcacgcttga tggttgacca
     2401 tttactgtgt cctgacatat gtttcctcgt tgcttaaccg cttctgcgtg taacaaataa
     2461 ggggctctta agcgcacttt aggcgtttgc agtggagctg agagccgtgc ccgcaagcgg
     2521 cgcggcagca gaagccttct ctgttatttt agcattatcc ggcttctttt tccaccatac
     2581 gacagccgtc aggaatacaa ttaccgcata gctgagaatc atgtaggcag tcgagaaacc
     2641 aaggttctgc gcaaaaacat gagggatgcg gctcagcaac tgcaccatgt ccagcatgta
     2701 gatcagtacg atggctgctg gccaggccaa ccaaccgacc agtgccggca aaagcatgcc
     2761 cgccaaaccg gctatgaggc tcagcaacat ggccaacggc actagggcag ctacaaccac
     2821 attagccacg ataccgatga acgacatctg gccgaaggta tggaggatga acggcagcgt
     2881 catgacttcc gcgcataaac tttccaacgc caccatgacg accagtgatt cccgccagcg
     2941 cggccgccag ttccggctca ccagcggtgc cacgaccatg acgccgtaaa acgccaggaa
     3001 gctcaggtac cagcttgtgt cggtccagac atagaatgga ctggcgaaac cggtgatggc
     3061 cgcagccagc aacagcaata caagcggctt gaattgccgg ccggagtagc ctgcagcgat
     3121 acttaacatg ctaactattg ccgcgcgcac aatgctggcg ctattgccgg cgaacagcag
     3181 gaagacggcg atcagtccga atgctaacag agtggtcaac cgcttgctgc gcctgccact
     3241 gacccgctcc gcactgcgta acagaatcgt caaattataa cccgacacag cgatgatatg
     3301 cgtcagaccg accatcaaca gcgcttgtga tactgccgcc ggcaggttgt tacgctggcc
     3361 tatcaagagt cccataccga agctggccaa cggctccggc aaggccgatt gcataccggc
     3421 tgtaaagcgc cgccgtagtt cgtttaccag cgaattatgg tgcgcggaca cctcgacctg
     3481 cgcatacgac atacggccct ggtatgagcc gagggaggat ttcagcttgc cggtagccgt
     3541 aacctcatca ccggcaaaga cggcattgta gccgaagccg ctcaggctat acttccctgc
     3601 cagcttgtcg ccggtagcca caaccgtcac atcactggcg tcgaaagcca gctgcccgta
     3661 cttgttataa gcggcatcct gcttggcgat ggccgtgatg gtcactttgc ggtcgtagta
     3721 ctgccctact tcggccattt tgagcatgta ctgcgagcca cgccaccagc cgagacttac
     3781 ac
//
LOCUS       DASSFD010000004         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1521395, whole genome shotgun sequence.
ACCESSION   DASSFD010000004 DASSFD010000000
VERSION     DASSFD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521395"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFH39_00070"
     CDS             complement(<1..862)
                     /locus_tag="VFH39_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924211.1"
                     /translation="MSDKLPCHNNPERLRDELIRAQREQAAIAQSLGESATAQAAALV
                     AIEQSGHRYEVEKAERTAQDRALVCRFLDALPAAANLAEHGPADPYLLNFASLAQAKV
                     AESLIWHEISGHNSHRIGYLVGQKAGRLPFRRRDERELLFAFRGPEKWTFDAKLVYDP
                     LRQKMEELALQQLAEEVDSHSISRYLSDDKSHHQLRYEKPWSWLPAGGRLMHLCEDER
                     ASSYPRKVLSPWVHNEEAAEKRLRNKGLRAYTQRRNITLDSSDIAAYSMDILCFRLAT
                     VLKLEPTYHGT"
     gene            932..2341
                     /gene="der"
                     /locus_tag="VFH39_00075"
     CDS             932..2341
                     /gene="der"
                     /locus_tag="VFH39_00075"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET6924212.1"
                     /translation="MSKKLPIVAIVGRANVGKSSLFNAILDRREAIVAREAGTTRDSI
                     SAKASWEGQDFWLVDTAGYKNPEDEFEFTIQEQIEQAADSADVVWVVVEADVPVTEED
                     RRVATAALKSRKPVFLVINKIDKVRHVDFAGFERLGIKPMVATTVKQGRGIEELLNRL
                     VNVIPKATMREDDDRLHIALLGRPNVGKSSLFNALAKKQQAIVADRAGTTRDINRAVV
                     RYEGREVELMDTAGIRRSGKIEVGVERFSVIRALSAIEQADACFLLMDANELNVQLDQ
                     KIAGMVKEAGKGLVLVVSKWDIAEDKDAYTRDALAPQIAANYEFVPWAPLIFTSSVTG
                     QNVTKLFDLAFQIDRARRKRIKTPELNRWLRQMLDKHPPAGLKGRSPKLNYMVQEENS
                     VPAFKIFGSQTSFVHWSYRRYLERGLREAYGFEGTPVQLWFIEKHIAHRHGDSPTQSA
                     RRSRLQKFIADKKARARKE"
     gene            2353..2997
                     /gene="pth"
                     /locus_tag="VFH39_00080"
     CDS             2353..2997
                     /gene="pth"
                     /locus_tag="VFH39_00080"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET6924213.1"
                     /translation="MGLFQRKPQVTDPIQLYYTVGMSNTLLIAGLGNIGTEYDGTRHN
                     IGFVCLDDFVRRNDDLGDWTEKKSLKCWLASGQLGQTRVICIKPTTFMNLSGEAVQAV
                     MNYYKITPDKLIVVHDELDIPFGQIRARHGGSSAGHNGIKSVTQHVGEEYGRVRIGIG
                     PKKPKEIDSADFVLQKFSAKELKDMPALSREVSAMLSEYAYGTKLAAETRSFLI"
     gene            complement(3031..>3345)
                     /locus_tag="VFH39_00085"
     CDS             complement(3031..>3345)
                     /locus_tag="VFH39_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924214.1"
                     /translation="DYCYGKIRTDNQIRLRQQRSFSVPAEVSIQIAMATADEFSVVAV
                     GLDARAVGLVGDKSREADVQRGKLMQRAAELSLFAANINKLCEPEIVARLLQELEQAT
                     QE"
BASE COUNT          758 a          983 c          963 g          641 t
ORIGIN      
        1 aggtcccgtg gtaagttggc tccagtttaa gcacggtagc taaccggaag cataagatat
       61 ccatgctgta ggcagctatg tcagaggaat ccaaagtgat attccgccgt tgagtgtagg
      121 cccgcagccc cttgttcctg agccgcttct cggccgcttc ttcattgtgc acccatggac
      181 tgaggacttt acgtggatag gacgaggccc gctcgtcctc acacaggtgc atcagacgtc
      241 caccggcagg taaccaggac cacggtttct cgtagcggag ctgatggtgg cttttatcgt
      301 cggacaggta ccggctgatt gaatggctgt ctacctcttc tgccagctgt tgcagcgcca
      361 gctcctccat tttttgccgc agcgggtcat agacgagctt tgcgtcaaaa gtccactttt
      421 cgggcccccg aaaagcgaaa agcagctccc tctcgtcccg gcggcggaac gggagacggc
      481 cagccttttg accgacgaga tagcctatgc ggtggctgtt atgcccgctt atttcatgcc
      541 agatgaggga ctcggctact ttggcctgcg caagggaagc gaagttaagc agataaggat
      601 ctgcaggccc gtgctccgct agattggcgg cagcaggtag cgcgtcgagg aaccggcaga
      661 cgagggccct atcctgggcg gtgcgctccg ccttttccac ttcatagcgg tggccggact
      721 gctcaatcgc gacgagcgcg gcggcttgag cagtcgcact ttctcccagg gattgtgcaa
      781 tggccgcctg ctcgcgctgc gcgcggatta attcatcgcg caagcgctcg ggattattat
      841 ggcatggtaa tttatcgctc attgataagt atgattatac aatactatat tctattttgt
      901 caatattacg acctctgtta taatagaagt aatgagcaag aagttaccaa tcgtagcaat
      961 agtgggacgg gctaacgtgg gcaagtccag cttgttcaac gcgattttgg ataggcggga
     1021 ggccatcgtg gcgcgcgaag ccgggacgac gcgagacagc atcagcgcca aagcctcctg
     1081 ggaaggacag gacttctggc tggtcgacac cgccggctac aagaacccgg aagacgagtt
     1141 cgagttcacc atccaggaac agatcgagca ggccgccgac agcgccgacg tcgtatgggt
     1201 ggtagtggaa gccgatgtgc cggttaccga agaagaccgc cgcgtcgcga ccgcggcgct
     1261 caagagccgc aaaccggttt ttttggtcat aaacaagatt gataaggtac ggcatgtcga
     1321 ttttgccggc ttcgagcggc tcggcatcaa acccatggtc gccaccaccg tcaaacaggg
     1381 aaggggcatc gaggagctgc tgaaccggct agtgaacgtt atacccaagg ccaccatgcg
     1441 cgaggacgac gaccggctgc atatcgcgct gctcggtcgg ccgaacgtag gcaagtcgtc
     1501 cttgttcaac gcgctggcca agaaacaaca ggccatcgtg gccgaccgcg ccggtactac
     1561 gcgtgacatt aaccgcgcgg tggtgcgcta tgaggggcga gaggtcgaac tgatggatac
     1621 cgccggtatc cgccgcagcg gcaagatcga agtcggggtg gagcgcttca gcgtcatccg
     1681 cgcgctatcg gccatcgagc aggccgacgc ctgcttcttg ctgatggacg ccaacgagct
     1741 gaatgtgcag ctcgaccaga agatcgccgg catggtcaag gaagccggca aagggctggt
     1801 actggtggtc agcaagtggg acatcgccga agacaaggac gcctataccc gcgatgcgct
     1861 ggcgccgcag atcgccgcca attatgaatt cgtaccgtgg gcaccgctca tctttaccag
     1921 cagtgtcacc ggacagaacg tcaccaagct gttcgacctg gccttccaga tcgacagggc
     1981 gcgccgcaaa cgcatcaaga cgcccgaact gaaccgctgg ctgcgccaga tgctcgacaa
     2041 acacccgccg gccggcctga agggccgcag cccgaaactg aactacatgg tccaagagga
     2101 aaacagcgtg ccggcattca agatcttcgg cagccagacc agcttcgtgc actggagcta
     2161 ccgccgctac ctggaacgag gcctgcgtga agcctacggc ttcgaaggga cgcccgtgca
     2221 gctgtggttc atcgaaaagc acatcgcgca ccgtcatggc gacagcccga cacagagcgc
     2281 ccgccgttcc cgcttgcaga agttcatcgc cgacaaaaaa gcccgggctc gcaaagagta
     2341 ggataaaaca taatgggact gtttcaacgt aaaccgcagg tgaccgaccc catccagctg
     2401 tattacaccg tgggtatgag caatacgctg ctgattgccg gattgggtaa catcggcacc
     2461 gagtacgacg gcacccggca caatatcggc ttcgtatgcc tggatgactt cgtgcgccgt
     2521 aacgacgacc tgggcgactg gaccgaaaag aagtcgctca aatgctggtt ggccagcggg
     2581 cagctgggcc agacgcgcgt catctgcatc aaaccgacca ccttcatgaa cctcagcggc
     2641 gaagccgtcc aggcggtcat gaattactac aagataacgc cggataagct catcgtcgtg
     2701 cacgacgagc tggacatccc cttcgggcag atccgcgccc gccatggcgg ctccagtgcc
     2761 ggccacaacg gcatcaagtc cgtcacccag catgtcggcg aagagtacgg aagggtacgc
     2821 atcggcatcg gtcccaagaa acccaaggaa atcgacagcg ccgacttcgt gctacagaag
     2881 ttcagcgcca aagaactcaa agacatgccg gcgctcagcc gcgaggtctc ggccatgctg
     2941 agcgaatacg cctacggcac caaactggcc gccgaaaccc gcagctttct aatttgatcc
     3001 acacgtctca ataaagtcgc gggccgcgat ttattcttgt gtagcttgtt ctagctcttg
     3061 caggagtcgg gcaacgatct caggttcgca cagcttgttg atattggcgg caaacaggct
     3121 caattccgca gcccgctgca tcagctttcc gcgctgcacg tccgcttccc gcgacttgtc
     3181 acctaccagt cccacagccc gagcgtccaa acccaccgcg acaaccgaga attcatcggc
     3241 tgtcgccatc gcgatttgga tactaacctc agctggtacg ctaaatgagc gttgctgccg
     3301 gagtcgaatt tgattatctg tcctgatctt tccatagcaa tagtc
//
LOCUS       DASSFD010000005         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1268740, whole genome shotgun sequence.
ACCESSION   DASSFD010000005 DASSFD010000000
VERSION     DASSFD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268740"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..646
                     /locus_tag="VFH39_00090"
     CDS             179..646
                     /locus_tag="VFH39_00090"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HET6924215.1"
                     /translation="MKYLIIRHAQTDSTRLNRHLDGPDGAPLNETGERAARALRDELT
                     AAGIEVQHVKVAISELVRTRQTAEAAGFVSENMRVDPLLNEVNTPDPAATQRLVEQGK
                     LPLEAFAAARAILDNPPEEPVWVTHGLVIAALRELLGLPGFIPPMASICEIEI"
     gene            863..2713
                     /gene="thrS"
                     /locus_tag="VFH39_00095"
     CDS             863..2713
                     /gene="thrS"
                     /locus_tag="VFH39_00095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET6924216.1"
                     /translation="MADQQPDDKLYAMRHSLAHIMATAIQRLWPETKFGVGPVVADGF
                     YYDVDLGEAKLSDEDFGKIEAEMKKVIESDVPFERFEKPVDEAIAWAREANQPYKEEL
                     LNDLKRSGTTVAKDLDADTLGVATEGDSAVENVSFYRDGDFTDLCRGPHVESTGRVGA
                     FKLMRVAGAYWRGNEKNAQMQRIYGVAFATDKELRQHLNMLEEAKKRDHRKLGQELDL
                     FLFSDMVGSGLPLFTPRGTVLREELARFSNELRERYDFQKVWIPHMTKKDLYEASGHW
                     AKFGDELFLVKSQETSDTLVMKPMNCPHHTRIYALQPRSYRDLPIRYLETTTIYRDEK
                     TGELNGLSRVRSATQDDSHVFCRHDQIEQEIDNLLACANELYATVGMDLRVRLSYRDD
                     SDAYLGSKGLWASAQEQLKNAVVANALNYYEQEGEAAFYGPKIDFMATDAIGREHQVA
                     TVQLDFVQPERFGLEYTGEDGMASVPVMIHCALLGSIERFLSVFIEHTAGKFPVWVAP
                     EQIRLISVNQEPATVEFTEKVRQQAKALGLRATVDNSNESVGKKIRAAEVWKVPYTVV
                     IGEKEMSGGEVTPRIRADLAVSEQAKSYTMDEFLQTVANEAKSRVNKSSL"
     gene            2716..3324
                     /locus_tag="VFH39_00100"
     CDS             2716..3324
                     /locus_tag="VFH39_00100"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924217.1"
                     /translation="MSSRKTIAVDVDDVLATSVQSWVAYSNQQWGTNLTVDDYDEDWA
                     KMWGVDQKEARQRALHLYESGVVGTFGHFEEAKAVLQRLAERYKLVITSSRVEHVRQH
                     TLEWLDDRFGGIFEEIHLAGIFDDGHPDAIKMTKSKLLQSIGANYLIDDHPKHCFAAA
                     AVGIPSLLFGEYVWNRNVTDLPSGVTRCLNWQEVGAYFDEKP"
     gene            3357..>3731
                     /locus_tag="VFH39_00105"
     CDS             3357..>3731
                     /locus_tag="VFH39_00105"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924218.1"
                     /translation="MNRLHVLEGVGDKRPLEVYDIDDVLLKSRDGFVKISHQEWGHDP
                     DIVKYKEEWDRWWGISHAETKKRSDYIWEQGLPLQFEPLEDAPEGLAMIARRARIVLL
                     TSRPIIAKEVTIASIERHFDTSC"
BASE COUNT          864 a          947 c         1216 g          704 t
ORIGIN      
        1 gcatcatttc agtactagca ttaaatggct ggtcctgtta agcttattgg taggtgaggt
       61 tttgcgatgt gtttcagtcc ttgctacact agtgggtaga aacgggcaca tcgaaaagta
      121 aaacggttgg atgacatgcg agatcaccca accgtttttt tattggtaag attaggtcat
      181 gaaatatcta ataatccggc acgctcaaac agatagtacg cggctcaacc gacatttgga
      241 cgggcccgac ggggcgccgc tcaatgagac gggcgagagg gctgcgcgtg cgctgcgtga
      301 tgaactgact gcggcaggca tagaggtgca gcatgtaaaa gtagcgattt ctgaactcgt
      361 gcgaaccaga cagactgctg aagcggcagg gttcgtgtcg gaaaatatgc gggtcgaccc
      421 gctattgaac gaagtgaaca cgccggatcc ggcggctacg cagcggttgg tagaacaggg
      481 caagctgccc ctggaagcgt tcgcggcggc gcgggcgata ctcgacaatc caccggagga
      541 accggtctgg gtgacgcacg ggctggtgat cgcggcctta cgcgagctgc tgggactgcc
      601 cgggttcatt ccgccgatgg ccagcatctg tgaaatcgag atttgagaag tgttcagcgg
      661 cgtgctacac tgacggcaag cgcggatgtg gactcaccaa ccacggagct tcgtctggag
      721 ggcaacgatg gacgtaaaac gaaagttgca agagtaaacg cttaggtgga acctcccggg
      781 aacgtccggg acccgagcac gctagagatt ggaatcatcc catcgacggc gtgttttttc
      841 gttataaata aggagataag ctatggccga ccagcagccg gacgataagt tatacgcgat
      901 gcgccactct ttggcgcaca tcatggcgac cgctatccag cggctgtggc cggagacgaa
      961 gttcggcgtg ggaccggtgg tggcggacgg cttctactac gacgtggacc tgggtgaggc
     1021 caagctttcg gacgaggatt tcgggaagat cgaggccgag atgaagaagg tcatcgagtc
     1081 tgacgtgcca ttcgagcggt tcgagaagcc ggtcgacgag gcgatagcgt gggcacgtga
     1141 ggcaaaccag ccgtacaaag aagagctgct gaacgacctg aagcgctccg gtaccaccgt
     1201 ggccaaagat ctggacgccg atacgctcgg cgtggccacc gaaggtgaca gcgcggtgga
     1261 gaacgtttcg ttctaccgcg atggcgactt tacggaccta tgccgcgggc cgcacgtgga
     1321 gagcactggt agggtgggag ccttcaagct gatgcgcgtg gccggtgcct actggcgcgg
     1381 caacgagaag aacgcccaga tgcagcggat ttatggtgtt gcgttcgcta ccgacaagga
     1441 gctgcggcag catttgaaca tgctggagga agccaagaag cgggaccacc gcaagctcgg
     1501 gcaggaactg gatctgttcc tgttcagcga catggtcggt tccggcctgc cgttgtttac
     1561 gccgcgcggc acggtgctgc gcgaggagct ggcccggttc tcgaacgaac tgcgtgagcg
     1621 ctacgacttc cagaaggtgt ggattccgca catgaccaag aaagatctgt atgaggcatc
     1681 gggccactgg gcgaagttcg gcgatgagct gtttctggtc aagagccagg agacgagcga
     1741 cacgctggtg atgaagccga tgaactgccc gcaccacacg cggatttacg ccttgcagcc
     1801 gcgcagttac cgcgacttgc cgatccgtta tctggagacg acgaccatct accgcgacga
     1861 gaagaccggt gagctgaacg gcctcagccg ggtgcgttcg gcaacacagg acgacagcca
     1921 cgtgttctgc cgacatgacc agatagagca ggagatcgat aacctgttgg cctgcgccaa
     1981 tgagctgtac gcaacggtcg gtatggacct gcgggtacgg ttgagctacc gcgacgacag
     2041 cgatgcgtat ctggggtcga aggggctgtg ggccagtgcg caggagcagc tgaaaaacgc
     2101 ggtcgtcgcc aatgcgctga actactatga acaggagggc gaggcggcct tttacgggcc
     2161 gaagatcgac ttcatggcga cggacgccat cggtcgggag catcaggtgg cgacggtgca
     2221 gctggacttc gtgcagccgg agcggttcgg cctggagtac accggtgagg atggcatggc
     2281 cagcgtgccg gtgatgatcc actgcgcgct gttgggctca atcgagcggt tcctgagcgt
     2341 gttcatcgag catacggccg ggaagttccc ggtgtgggtg gcgccggagc agatccggct
     2401 catcagcgtg aaccaggagc cagcgacggt tgaattcacc gaaaaggtgc ggcagcaggc
     2461 caaggccctc ggtttgcggg caacggtcga caacagtaac gagtcggtgg gcaagaagat
     2521 ccgcgccgcc gaagtctgga aagtgccata cacggtggtg attggcgaaa aagagatgtc
     2581 cggcggcgag gtgacgccgc gcatccgcgc cgacctggcg gtttccgagc aggccaagag
     2641 ctacactatg gacgaattcc tgcagacggt cgccaacgaa gccaaaagcc gggtgaataa
     2701 gagcagcttg tagctatgag tagccgcaag acaattgccg tcgacgttga tgatgtactc
     2761 gctaccagcg tgcagtcctg ggtggcgtac agcaaccaac agtggggcac gaacctgacg
     2821 gtggacgatt acgacgagga ctgggccaag atgtggggcg tcgaccaaaa agaagcacgc
     2881 cagcgggcat tgcatctgta tgaatcaggt gtcgtcggta cgttcgggca ctttgaagag
     2941 gctaaagcgg tgctgcagcg cttggcggag cggtacaagc tggtcattac ttcctcgcgc
     3001 gtcgaacacg tgcggcagca tacgttggag tggctggatg accggttcgg tggaatcttc
     3061 gaggagatcc acttagcagg tatcttcgat gacgggcacc cggacgccat taagatgacc
     3121 aaaagcaaac tgttgcaatc catcggcgcg aactacctca tagacgacca cccgaaacac
     3181 tgcttcgccg cggccgctgt cggcattccg tcgctgttgt tcggcgaata tgtgtggaac
     3241 cgcaatgtaa ccgatttgcc cagcggcgtg acgcgctgtc tgaactggca ggaagtggga
     3301 gcgtacttcg atgaaaagcc atagtatttg cgcctcatgc taaaattttg atgaagatga
     3361 acagattaca tgtgctggaa ggagtgggcg acaaacgtcc gctcgaagtg tacgacatcg
     3421 atgatgtctt actgaagtcg cgcgacggtt tcgtaaagat cagccaccaa gagtggggcc
     3481 acgaccctga tatagtcaag tataaagaag aatgggaccg gtggtggggc atatcgcacg
     3541 ccgaaacgaa gaagcgtagc gattatatat gggaacaggg gctacccttg caatttgagc
     3601 ccttagaaga cgctccggaa ggccttgcaa tgatagcccg gcgggcacgc atcgttctct
     3661 tgacctcacg gccgattatc gccaaagaag tgactatcgc atcgattgag cggcatttcg
     3721 atacatcttg c
//
LOCUS       DASSFD010000006         6186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_886682, whole genome shotgun sequence.
ACCESSION   DASSFD010000006 DASSFD010000000
VERSION     DASSFD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886682"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..610)
                     /locus_tag="VFH39_00110"
     CDS             complement(29..610)
                     /locus_tag="VFH39_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HET6924219.1"
                     /translation="MGIYSGTFNPVHAGHIAFALQAIQTAKLDKLYFLPERKPRYKVG
                     VEHFAHRVAMLKAAARPHARLAVLELHDISFSVQRTLPQLQQRFPGSQLVFVVGSDVA
                     QHLPDWPHGDRLLAQSEVVIGLRTPQNRAEIERLVGSWPQQPLGTHIIDSFAPHISSG
                     TVREALRTRRYTAGLLRSVAQYSNRHWLYVSVA"
     gene            complement(684..884)
                     /locus_tag="VFH39_00115"
     CDS             complement(684..884)
                     /locus_tag="VFH39_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924220.1"
                     /translation="MQDKPSVDVDDEIYQELVGQQSPEFFCDNCGFYPIQPVHGHFMC
                     PQCHMPTKCCEGMPLELGDAPA"
     gene            complement(930..2480)
                     /locus_tag="VFH39_00120"
     CDS             complement(930..2480)
                     /locus_tag="VFH39_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924221.1"
                     /translation="MAVAAESQQQRWQLARDAFPEAEYEAERKLTAYIGSIAVRGEHQ
                     LHSPPEDDNESTLLHAVQLAALGDTDSRYVVETCVRTDYSERLYKVANRSRVELSFIG
                     NELWQQGRSLQSIHGNTLRHMHLTPEMLKRTMLETRNAGLFGELYARGALQDHAAVVF
                     STTSTKMSQTEKKDYNFFLDTETCSIQLLTADGAAATLETALVAGKSMPSGERHDLNT
                     INKLSRGQGIAIHENDGTDMLRHVMLIPKTAVPNGVIDVVRMYDDAAGGTFYGQAQPR
                     QDYISHARACEQRNRLFDNTVQQITEQLIREAAHFKTPIEAIERLDFLSERHGVRQAV
                     HDTSIDARVFGAAAVMHIEEARFFAAQGDTERADRSMRAAQKVASSGSCPIFKGAERS
                     SQTDDVDNQSSQEESSGKKVMSCPFCSAKVYDDPCASVLTCWDCRAQVVNGRVVNKGN
                     GGRKAREARKAAEAARKAEHKKHGEAALGEQVDAVFEERGLEFPTEPEVKPVKAAAQL
                     AVAAAQSH"
     gene            complement(2691..3368)
                     /locus_tag="VFH39_00125"
     CDS             complement(2691..3368)
                     /locus_tag="VFH39_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924222.1"
                     /translation="MYRLGERKHSRRKLYAWLGGFLVLLFAGAIVAVYLLHADTVISQ
                     TPAPVVRRLTYAAPKTTVYEGATFKISLPEGWKTMAVTDMPTPTYTWHGTGKTDGNRW
                     LDVYVDADLTTLSLNRAVSVAAHGNGVSATSDVSDNCASFIGNAKPAPHQTYVPAKWH
                     DLPFLCETGTYARDVVGTVSADGLNNVTISGPASGKHHYMFTYTDNSASPDYKVFTDA
                     LQTFQAK"
     gene            complement(3424..3915)
                     /locus_tag="VFH39_00130"
     CDS             complement(3424..3915)
                     /locus_tag="VFH39_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924223.1"
                     /translation="MNEKQTIEPTAVRPTKKQRELLTYIEGFISEHGYSPSYREIMNG
                     LNYTSVATVALHVNSLIKRGHLRKRDRSARSLEVVTPSGEPAKVTSNQVSDSEEKWLV
                     QKIEHAFAQAEQAGEQLLQTTVDELYVLVGALRVLGLEGAAQSFIPRLSELKKRSTVA
                     AGQ"
     gene            3952..4524
                     /locus_tag="VFH39_00135"
     CDS             3952..4524
                     /locus_tag="VFH39_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924224.1"
                     /translation="MSSVTTFEIFHDIDRGKFPTESVEAAITYIGEIIGEHTILSNRS
                     VRLSSNPSSEYIDMEKIRWPKFSADVNIVLTDRALDKPPEGSDVALLDLTRRITTGVS
                     NPLSKVAVIDTLRTTAEAATVTHEIGHLFNLKLKGITKSTQSPHCALNLCYARKSRTV
                     IHKKRSDNSADLFRAHRSSQETEAFLHRGA"
     gene            complement(4627..5067)
                     /locus_tag="VFH39_00140"
     CDS             complement(4627..5067)
                     /locus_tag="VFH39_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET6924225.1"
                     /translation="MTVSLKERFFGNRPDRRTFAAQFGKYMAGGALYFWVGYAVFAIC
                     YSGFHWSWLPAKILADAIGWSLNYLVQRFWAFKDQVHLSEMQHAGRYVFIESIGFVLD
                     YLIIWGLNAIGITPYIGFFISSGFFTVWSWLWYKYWVFPEQKRT"
     gene            complement(5064..>6186)
                     /locus_tag="VFH39_00145"
     CDS             complement(5064..>6186)
                     /locus_tag="VFH39_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6924226.1"
                     /translation="LRQRFLRSRLIQLILLYTLVQLVWGFVAYGMHHVTPKAMGYGLI
                     VNLRLPAFFAVTWVLAQKSHTLPKRWRPLVYGPAIAVIVIGLLQYFVLPYDFLRHFGY
                     GPDTINPYETINHNVHYIRAMSTLRGANPLGAYLVLILSLLFVEWRRQWRDWQFAILP
                     VAGLIVLVLSFSRAAWVGLLLAGAVVGSTMLKTRTAQRTTALVLTALVVIAAGLFAGF
                     RHNTTFQNVFAHTQDHSLVATTSNGGHTSALRSGAHDVLHEPLGRGVGTAGPASIYND
                     NKGRIAENYFIQIGQETGWIGLLLFISICVVLGRELWLRRQDDLALGLLAALVGLSFV
                     NLLSHAWADDTLAYVFWGLAGIALAKPIVKTKTETKHAK"
BASE COUNT         1330 a         1686 c         1752 g         1418 t
ORIGIN      
        1 gtcaacgatt aatgacgttt tgcgaatttc aggctacaga aacgtacagc cagtggcggt
       61 tactgtactg ggcgacgctt cgcaacaggc cagcggtgta gcggcgagtg cgtaaggcct
      121 cacgaacggt gccggaagag atgtgaggag cgaagctatc gatgatatgg gtgcccaaag
      181 gttgctgagg ccagctgccg acgagccgtt cgatctccgc acgattctga ggtgtccgca
      241 gacctatgac tacctcgctc tgagcaagca ggcggtcacc atgtggccag tcgggcaggt
      301 gctgtgcgac atcagaaccg actacgaaga ccagctggct gcctggaaaa cgctgctgta
      361 actgaggcaa agtacgttgc acgctgaagc tgatgtcgtg cagctccaga acagctaacc
      421 gggcgtgcgg ccgggcggcg gctttcagca tggcgacgcg gtgggcaaag tgctcaactc
      481 ccactttata ccgcggcttg cgctctggca gaaagtacag cttgtcgagc ttggccgtct
      541 gtatggcctg tagcgcaaag gcgatatgcc ccgcatgtac cgggttgaaa gtgccgctgt
      601 aaatacccac tcgagacaaa cctacggttt ttctcgacgc gcggtgggcg gaatgaatcc
      661 gcttcgccgg ctgctctttt cccttacgca ggtgcatcac ctaactccag gggcattcct
      721 tcgcagcatt tggtgggcat atggcactgc gggcacatga agtgcccgtg caccggctgg
      781 atggggtaaa agccgcagtt atcgcagaaa aactccgggg attgctgccc gaccagttcc
      841 tggtatattt catcatcgac gtcgacgcta ggcttgtctt gcatgccatt aattatatgc
      901 ctagtgcttg aatgagactg tagtattctt taatgactct gtgctgcggc gacggccagt
      961 tgggctgctg ctttgactgg ttttacctca ggctccgtgg gaaattccag accacgctct
     1021 tcaaacactg cgtcgacctg ctcgcctaag gctgcttcgc catgcttttt atgctccgct
     1081 ttgcgggctg cttcagcggc tttcctggct tcgcgagcct tacggcctcc gttccctttg
     1141 ttgacgactc taccgttcac gacctgtgcc ctgcagtccc agcatgtcaa gacagaggcg
     1201 cagggatcat cgtatacttt ggcgctgcag aacgggcagg acatgacttt cttgccggat
     1261 gattcttcct gtgatgactg attgtcgacg tcgtccgtct gactgctgcg ctcggcaccc
     1321 ttgaagatcg ggcaactccc cgaggacgcc actttctggg cagcccgcat tgaccggtca
     1381 gcccgctcgg tatcgccctg ggccgcgaag aagcgagcct cttcgatatg catgacggct
     1441 gcagccccga agactctggc atcgattgag gtgtcgtgca ccgcctgacg gacaccgtgg
     1501 cgctccgaca ggaagtcgag acgctcaatc gcttcaatcg gtgttttgaa gtgcgcggct
     1561 tcgcgtatga gctgttcggt gatttgttga acggtgttgt cgaacagccg gttgcgctgt
     1621 tcacacgccc gagcgtggga gatgtaatcc tgtcgcggtt gagcttgacc gtaaaaagtc
     1681 ccgcccgccg catcatcgta catcctgacg acgtcaatta cgccgttcgg tacggcagtc
     1741 ttgggtatga gcataacgtg tcgcaacatg tccgtaccgt cattttcgtg aatagcgatg
     1801 ccctggccgc ggcttaactt gttaatcgtg tttaagtcgt gtcgttcacc ggagggcatc
     1861 gatttgccgg ccactaaggc ggtctcgagc gtagccgcag cgccgtcagc tgtcagcagc
     1921 tggatggaac aggtctcggt atcaagaaag aagttgtaat ctttcttctc ggtttggctc
     1981 atcttcgtgg aggtggtgga gaataccacg gcagcgtgat cttgcagcgc tccccgggca
     2041 taaagctcac cgaacagtcc cgcgttgcga gtctcaagca ttgttcgctt gagcatttcg
     2101 ggggtcagat gcatgtgacg gagcgtgttg ccatggattg attgcaggga tcgtccctgt
     2161 tgccacagtt cattgccgat gaagctcagc tcgacccggc ttcggttcgc aactttgtac
     2221 agccgctccg agtaatcggt tctcacgcag gtctcgacga cgtacctcga gtcagtatca
     2281 cctaatgccg ccagttgtac tgcatgcagc aacgtcgact cattatcgtc ctcaggaggg
     2341 ctgtgtagct ggtgttcacc tcggacagcg atggagccga tataagcagt aagtttgcgc
     2401 tcagcctcgt attcggcttc cgggaaggca tcccgggcaa gttgccagcg ctgttgctga
     2461 gactcggctg cgacggccat aaaaataccc tctgctccga tgagcgagag ggccttcaaa
     2521 ctgtcggctg ctcctgctcg ggagactttt agaaccgtat ctggctataa cagttgcggc
     2581 acagctcagg acttacacct gattccggtt gatgttgcta ttaatgtgtc tagtagtgta
     2641 gcactcaccg cttacgctag atatagtatt tttaacgcca gccggtccgc ttacttggct
     2701 tggaacgtct ggagcgcgtc ggtaaatacc ttatagtccg ggctggcgga attgtcggtg
     2761 taggtaaaca tgtagtggtg cttgccactg gccggtccgc taatcgtaac gttgttcagc
     2821 ccgtcggcag ataccgtccc cacgacatca cgggcgtacg tgccggtttc gcacaaaaac
     2881 ggcaagtcat gccacttggc cggcacatac gtttggtgtg gggcgggctt ggcgttgccg
     2941 ataaagctgg cgcaattatc ggacacatca cttgtggcac ttacgccatt gccgtgggcg
     3001 gctactgaca ctgcacggtt aagcgagaga gtggtgaggt cggcatcgac gtatacatct
     3061 agccagcggt taccgtctgt cttaccggtg ccgtgccagg tgtaggtcgg agtgggcatg
     3121 tcggtgacgg ccatggtttt ccacccctcc ggcaaactta ttttgaaagt agctccttcg
     3181 tacaccgtcg ttttcggcgc ggcgtacgtc agccgccgca cgacaggagc aggcgtttgg
     3241 ctgatgacgg tatcggcatg tagcaagtag acggctacga tggcgccagc gaacaacaag
     3301 accaaaaatc cgcccaacca agcgtacagt ttgcgtcgac tatgtttgcg ttcacctaag
     3361 cggtacatat ttgtttatgc ttaacatgtt tagtatagcg tatccgaggg ctgggtagca
     3421 ttgctactgc ccggcggcta ctgtcgagcg ctttttaagc tcgcttaagc gagggatgaa
     3481 gctttgggct gcaccctcca ggcctaacac ccggagcgct ccgaccagca catacagttc
     3541 atcgacagtg gtctgcaaca gctgttcgcc tgcctgctcg gcctgagcga acgcatgctc
     3601 tatcttctgg accaaccatt tttcttcgct gtccgacact tggttgctcg taaccttggc
     3661 cggttcgccg cttggcgtca ccacttccaa cgaccgggca ctgcggtccc gcttgcgcag
     3721 atgaccacgt tttataagac tgttcacgtg cagcgccacc gtggctaccg acgtgtaatt
     3781 gagcccgttc atgatttcgc gatagctcgg gctgtagccg tgctcggaga tgaagccttc
     3841 tatatatgtc agcagctcgc gttgcttctt ggtgggccgt acagcggtag gttcgattgt
     3901 ttgtttttcg ttcatgctgt tattatgaca tacattcgca atagtaaaat catgagtagt
     3961 gtaacgacat ttgaaatctt tcatgacatc gatcgcggca agtttccgac cgaatcggtt
     4021 gaagccgcta taacgtacat cggtgaaatt attggagagc acactatctt aagcaacaga
     4081 agcgtgcgct taagtagtaa tccgagcagt gagtatatag acatggaaaa gattcgttgg
     4141 cctaagttct cggccgacgt caatatagtc ctgacggatc gagctcttga taaaccgcca
     4201 gaaggatcag atgtcgccct acttgatttg acgaggcgaa taaccacggg agtctccaat
     4261 ccattgtcca aagtggccgt tatagacaca ctcaggacga ctgctgaagc tgctactgtc
     4321 acccatgaaa taggtcattt gtttaaccta aagctcaaag gcattactaa aagtactcag
     4381 tcaccgcact gcgctcttaa tttgtgttat gcacgaaagt ctcgaacggt catacacaag
     4441 aaaagaagtg acaatagcgc cgaccttttt agagcgcatc ggagtagtca agaaacagag
     4501 gcattcctcc accgaggcgc ataatccggt gatagtgttc tgcgaagagt gtacgcagca
     4561 gctgggatct accgccctat tacggaactt ggccaaaagc ggtcgtcatg tcccggactt
     4621 tttgctttaa gtgcgctttt gttccggaaa gacccagtac ttgtaccata gccagctcca
     4681 cacggtgaaa aagccgctcg atatgaagaa gccgatgtac ggcgtgatgc cgatggcatt
     4741 gagaccccag ataatcaggt agtcgagcac gaagccgatg ctttctatga acacgtagcg
     4801 gccggcatgc tgcatttcgc tgaggtgcac ctggtctttg aacgcccaaa agcgctgcac
     4861 caggtagttc agcgaccaac cgatggcgtc ggccaggatc ttggctggta gccaggacca
     4921 gtgaaagccg ctgtaacaga tggcaaatac ggcgtaaccg acccagaagt acagcgcgcc
     4981 gccggccatg tatttgccga actgggcagc gaatgtacgc ctgtcgggtc ggttgccgaa
     5041 gaatcgttcc ttgagcgaga cagtcattta gcgtgcttcg tttcggtctt ggtttttacg
     5101 attggttttg ccagagcgat gccggccaag ccccagaaga cgtaggccag cgtgtcgtcg
     5161 gcccaggcgt ggctgagcag gttcacgaag ctgaggccga ccagcgcggc cagtagcccg
     5221 agcgccagat cgtcctgccg acgcagccac aactcgcggc cgagtacgac gcagatggaa
     5281 atgaacagca gtaacccgat ccagccggtc tcctggccga tctgtatgaa ataattctcg
     5341 gcaatccgtc ccttgttgtc attgtatatc gaagccggcc cggccgttcc gacgccccgt
     5401 cctagcggtt catgcagcac gtcgtgcgcc cccgaccgca gtgcggaagt gtggccgccg
     5461 ttgctggtag tggccacgag cgagtggtcc tgggtatggg caaagacatt ctggaacgta
     5521 gtgttgtgcc ggaagcccgc aaagagtccg gctgcgatta ctacaagagc tgttagcacg
     5581 agggccgtcg ttcgctgggc cgtacgggtt ttcaacatcg tggaaccgac gactgcccca
     5641 gccagcaaca ggccgaccca agcggctcgc gaaaagctca gtaccagcac gatcagaccg
     5701 gcaacaggca gaatagcgaa ttgccaatcg cgccattgcc ggcgccactc aacgaacagc
     5761 aaactcagta tcagaaccag ataggcgccg agcggattgg cgccgcgcag cgtcgacatc
     5821 gcacggatgt agtgcacgtt gtggttgata gtttcgtatg gattaatcgt gtccggtccg
     5881 taaccgaagt gccgcagaaa gtcatacggc aggacgaaat actgcaacag cccgatgact
     5941 attaccgcta tggccggccc ataaaccagc ggacgccagc gcttgggtaa tgtgtggctt
     6001 ttttgtgcca acacccaggt caccgcaaag aacgccggca ggcgcagatt cacaatcagt
     6061 ccgtagccca tcgccttggg cgtcacgtgg tgcatgccgt acgctacaaa gccccatacc
     6121 agctgcacga gcgtataaag caggatcagc tgtatcaatc gggagcgaag aaagcgctgc
     6181 cgcagg
//
LOCUS       DASSFD010000007         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1776125, whole genome shotgun sequence.
ACCESSION   DASSFD010000007 DASSFD010000000
VERSION     DASSFD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776125"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFH39_00150"
     CDS             <1..353
                     /locus_tag="VFH39_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924227.1"
                     /translation="SLTDGTGNDAERQTDIRALETQLEAFYAQNGYYPDLNDLNNPQW
                     VSTNLTGIDKEVLKDPQGSGYTLAAKPAAHVYAYQPTSTAGGVCSEAKQDCQKYTLTA
                     TLSNGSSYTKASLN"
     gene            361..1629
                     /gene="murF"
                     /locus_tag="VFH39_00155"
     CDS             361..1629
                     /gene="murF"
                     /locus_tag="VFH39_00155"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET6924228.1"
                     /translation="MKQFAKSIVLRVLKGQVRRLRKRHKVVVVAVAGSIGKTSTKFAV
                     ARLLGATKRVRFQEGNYNDIVTVPLVFFGTPLPSLFNPLAWVRTFWRNERQIHGNYAY
                     DVVVVELGTDGPGQLARFKDFIAVDIGILTAIAPEHMEFFDSLRDVAREELVISDLAK
                     TLLINSDLCAPEYIPQRQMCYRYGIDQAAAYTMQDLVFDRTGYSFTVAFEGKRLLQAS
                     HPSIAIPQLYSLLAAVAVANQLGMSEADIRQGLAAIAPVSGRMQLLRGLQDSTIIDET
                     YNASPEACKAALRTLYQLEAPQKIALLGSMNELGAYSPAAHAEVGAFCDPKQLALVVT
                     LGSDANQYLAPAAEERGCTVVRVASPYEAAGVIKQALKQGAAVLAKGSQNGVFAEEAV
                     KLLLADPTDANKLVRQSASWQKIKAKQFSR"
     gene            1722..>2663
                     /locus_tag="VFH39_00160"
     CDS             1722..>2663
                     /locus_tag="VFH39_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6924229.1"
                     /translation="MNNPDEELTVQRYNPRDIESKWQAEWEQSGIYQASEDPTKPKKY
                     ILEYFPYPSGAAMHVGHVRNYTIGDALARFNRMLGYNVLHPMGWDGFGLPAENYALKT
                     GISPRQAIDENTTRFKQQLMQMGFSYDWSREIDSTDPRYYRWTQWFFLMLFKRGLAYQ
                     QDSLQWWCPVDKTVLANEQVEAGHCWRCGNIVEKKALKQWFFKITEYADRLEKDLDDV
                     NWSESIKSMQRNWIGRSKGAEVEFAIPSANTSVKVFTTRPDTLFGATFLVLAPEHPLV
                     GTIATDEQRSEVEAYIRATQAKSDVERQETNREKTGVF"
BASE COUNT          616 a          705 c          773 g          569 t
ORIGIN      
        1 gctcgttaac ggatggaacc ggcaatgacg ctgaacggca gactgacatt cgggcgcttg
       61 aaacccagct tgaagctttt tatgcccaga atggctacta cccggacctg aatgatctca
      121 ataacccgca gtgggtatca accaacctga caggtattga taaggaagta ttaaaggatc
      181 cgcaaggctc cggctatacc ctggccgcca aaccggctgc ccatgtatat gcctaccagc
      241 ctacttccac tgccggcggg gtatgctctg aggcaaagca ggactgccaa aaatacacct
      301 taaccgcaac cctaagtaac ggcagcagct acactaaagc cagtctcaat tagccgatag
      361 atgaagcagt tcgccaagag catcgtttta cgggtcctca agggtcaggt gcgccggttg
      421 aggaaacgtc ataaggtggt cgtggtggct gttgctggca gcatcggcaa gacgagtacc
      481 aaattcgccg tggcccggct gctcggcgcc accaaacgag tccgctttca ggagggtaat
      541 tataacgata tcgtgacggt accgctggtg tttttcggga cgcccttgcc gagcctgttc
      601 aatccgttgg cgtgggtgcg cacgttctgg cgcaatgaac gccaaataca cggtaactac
      661 gcttatgatg tggtggtggt tgagctgggt accgacgggc cgggccagct ggcgcgtttc
      721 aaagatttta tcgccgttga catcggtatc ttgacagcca ttgctccgga acatatggag
      781 ttctttgatt cgcttcgcga cgtagcgcgc gaggagctgg taatcagtga ccttgcaaag
      841 acgctgctta taaacagtga cctgtgtgcg ccggagtata taccccagcg gcagatgtgc
      901 tatcgctacg gcattgacca ggctgcagcc tataccatgc aggacctggt gtttgaccgg
      961 accggctaca gtttcacggt agcgtttgaa ggcaagcggc tgctgcaggc ctcgcacccg
     1021 agcattgcga ttccgcaact gtactcactg ctggcggcag tggctgtcgc caaccagctg
     1081 ggtatgagcg aagccgacat tcggcagggc cttgctgcta tcgcgccggt cagcggccgg
     1141 atgcagctgt tgcgcggttt gcaggatagc accatcatcg acgagaccta caatgccagt
     1201 cccgaagcct gcaaagcggc gctgcggacc ctgtaccagc tggaagcgcc gcaaaagatc
     1261 gccctgctcg gtagtatgaa tgagctggga gcgtattcgc cggccgccca tgcggaagtc
     1321 ggggcgtttt gtgatcctaa acagcttgcg ctggtcgtca ccttggggtc ggatgccaac
     1381 cagtacctgg cgccagcggc tgaagagcgc ggctgtacgg tggtacgggt tgcatcgccg
     1441 tatgaagcgg caggcgttat caaacaggca ctcaaacagg gggcagctgt gttggccaag
     1501 ggttcgcaaa acggcgtttt tgccgaagag gcggtcaaat tactgctggc tgatccgacg
     1561 gatgcaaaca aactggtccg tcagtcggcc tcatggcaga aaatcaaagc caagcaattc
     1621 tcccgctaat ctgttatagt agctggtaat ggcacgcttg catcttcttg gtctactcct
     1681 cgcccgctag ggcagtttct tttctttgac ttataatacg aatgaacaat ccagacgagg
     1741 agttaaccgt gcaacgatac aatccgagag acatcgagtc caaatggcag gctgagtggg
     1801 aacaatctgg tatttatcag gctagtgaag acccgacaaa acccaaaaaa tatatcctgg
     1861 aatatttccc gtaccccagc ggggcagcta tgcatgtcgg gcatgtccgt aactacacga
     1921 tcggcgatgc gttagcccgt ttcaaccgca tgctcggtta taacgtcctg catccgatgg
     1981 gctgggacgg ctttggtctg ccggcggaga attacgcact caagaccggt atcagtccgc
     2041 gccaggcgat tgatgagaac accacccgtt tcaagcagca gctgatgcaa atggggttta
     2101 gttacgactg gtcacgcgag atcgattcga ctgatccgcg ctattaccgc tggacccagt
     2161 ggtttttcct gatgctgttc aaacgcggcc tggcctatca gcaggatagc ctgcagtggt
     2221 ggtgtccggt cgataagacg gtactggcca acgagcaggt tgaagccggc cattgctggc
     2281 gctgtggcaa catcgtcgag aaaaaggcgc tcaagcagtg gttcttcaaa atcaccgagt
     2341 atgctgaccg attagaaaag gatctggacg acgttaactg gagcgagtcg atcaagagca
     2401 tgcagcgcaa ctggattggc cgcagcaaag gtgcggaagt cgaatttgcg attccgtctg
     2461 ccaatacttc tgtcaaagtc tttacgacca ggccggatac gctgtttggc gccaccttcc
     2521 tggtactggc gcccgagcac ccgttggtgg ggacgattgc gacggatgaa cagcgcagcg
     2581 aagtcgaggc gtatattcgg gccactcaag ccaagagcga tgtcgagcgc caggagacta
     2641 accgcgaaaa gaccggtgtc ttt
//
LOCUS       DASSFD010000008         7343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1772993, whole genome shotgun sequence.
ACCESSION   DASSFD010000008 DASSFD010000000
VERSION     DASSFD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772993"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1246)
                     /locus_tag="VFH39_00165"
     CDS             complement(59..1246)
                     /locus_tag="VFH39_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6924230.1"
                     /translation="MAKILLVEDDNNLREIYEARLQAEGYDIVSASDGEEALVVAKKE
                     RPDLVISDVMMPKISGFEMLDILRNTDGLKDVQVIMLTALGQAEDRTRADQLGADKYL
                     VKSQVTLEDIVKTAHQLLDDEPAAPAATEADDEAAPAAAAPEPAPVQPAPQPVAPAPA
                     EPVAAAPAPEPAASQAAPVAAPPADDTSAAATTAAETAAMEAQIESFVSTPTPQTAAA
                     EPASAEPAPAAATPAAPPAELVAPATEPAASATSPSNQPAPADDQSSNDQVMNDALAS
                     LNTTGSEPGKPSDVTAVEVPPEPAPAAASEPELATTPAKPDSTTAGGKKVIQPLNDVD
                     SKKDLSDLLAKEAASEPVAMPDANAVVTPPPEPPTPPAGQTPPATPPQQPPTGPDPNS
                     IAL"
     gene            complement(1313..2554)
                     /locus_tag="VFH39_00170"
     CDS             complement(1313..2554)
                     /locus_tag="VFH39_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6924231.1"
                     /translation="MAWFKRTNQGSDKLADRLRDEKLKSSIILNAIEDGVVLLDDQGA
                     IHVFNPGAATITGWTIEEASGLDWRSVFKFVNDKGEPIDESAVPITTAMTNGKSVRAN
                     DANLLTKSGRIIALSFAASPLLDDQQNVTGVVGIFRDVSEERQEESRRADFISTASHE
                     MRTPVAAIEGYLSLALNDKVSTIDMKARGYLEKAHASTQHLGQLFQDLLTSAKAEDGR
                     LTNHPQVVEMGAFLDQLVQDLKFSAEKKGLFMDFNFGSAAQAVNTNAKVVLPLYFVHV
                     DPDRIREVITNLFDNAVKYTDEGKISLGITGNDQVVQIYIRDTGPGIPAEDIPHLFQK
                     FYRVDNSATRTIGGTGLGLFICRKIVELYQGRIWCESELAKGTTFFINLPRLSSQQAK
                     QLQTEEAANAPLAPIPTATGL"
     gene            complement(2765..3964)
                     /locus_tag="VFH39_00175"
     CDS             complement(2765..3964)
                     /locus_tag="VFH39_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924232.1"
                     /translation="MRLGREQLKRRATETVIVVTIALIGSLLVLLTHAQTDTASLEAE
                     AGTLNGKAVSVKDAKASGGEAVKFTSGDKCTISPILVNSCRPWLGGHAAHYPPPVVAN
                     LTGQTLDHEKRIGRQLDVVRGNYHNFDDPLITGEQAFATRPNTLLFISWKPAATFQEA
                     ADSSNATTNLAIDHMAASIKALGSHKIFLTLWHEPQLEVTAGSVPGCSSGAYRSGATG
                     GTPAQYRAMWANVENRFKADGVTNVVWSIVYLGGPKATCMIDDLWPGNNLIDWVDFDR
                     YGSKNYDTWQSTVGTTYRFLTSASDAAHDYLSKPWGVAEFSISNVDQPTAYKYWDSIK
                     QALDANTFPKIKLYMVYDSAGGPGGAPTPAGDNRVEYGLNGFDPIEQQHYNGFADDPR
                     FTDAFYQ"
     gene            complement(4102..4178)
                     /locus_tag="VFH39_00180"
     tRNA            complement(4102..4178)
                     /locus_tag="VFH39_00180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4142..4144),aa:Glu,seq:ctc)
     gene            4391..5044
                     /locus_tag="VFH39_00185"
     CDS             4391..5044
                     /locus_tag="VFH39_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924233.1"
                     /translation="MPFKNRHVPHDPVWPVQLAMLVAIALQVALPDTFSAARYAIPAA
                     ELLLLIALSFTTPKERIFRSMSRRINVILLIALTSAANAYSLGVVCNRLLQGGHVSNG
                     RELILTSINIFLTNIIIFGLWYWEMDGGGPGERSHIAKYEQDFLFPQHHNEDYKHPDW
                     KPTFLDYLYVSSTNAMTFGPADTKPLSRRAKMLMLLQSTISLIVVALVAARAVGILN"
     gene            complement(5045..5473)
                     /locus_tag="VFH39_00190"
     CDS             complement(5045..5473)
                     /locus_tag="VFH39_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET6924234.1"
                     /translation="MKLRIVTIGKPKLHYAQEGWEEYLPRLSRFHKVSVSQLADKYTG
                     DAARILEAATGYKVALVINGATMSSPQLAGFLRERELAAQEVSFIIGGPEGLPSAVIE
                     ACDYRWSFGPLTFPHDLAMIILLETLYRASTINAGMPYHK"
     gene            complement(5470..6081)
                     /locus_tag="VFH39_00195"
     CDS             complement(5470..6081)
                     /locus_tag="VFH39_00195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HET6924235.1"
                     /translation="MKIEHLLSSHPLISDQVSKAQVAVILEELERVGEQGTPGAVVEF
                     GCYIGTTSLFIRRLLDALGDDGRELHVYDSFEGLPPKTTADASPAGEQFKAGELSVSK
                     KQFLTEFKKAGLRPPVIHKGWFNDLTTTDVPETIALAFLDGDFYTSIRDSLKLVLPRM
                     QPGGAIVIDDYAREALPGVARAAHELLPGKQIRTAHDLGIVRL"
     gene            6170..7147
                     /locus_tag="VFH39_00200"
     CDS             6170..7147
                     /locus_tag="VFH39_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6924236.1"
                     /translation="MEKQPGDATGRPGWQHPSADERRQARLWEKEYGGSQAGLVNRWS
                     YYRHALHGEVIYYWEAYRRLEADPSGSLLDVGCSDGVSLSVIRALQEQGGHSGPIVGV
                     DLKRVLVGNRAAKRLTRMFYDNNVLPIQFVVGSGDHLPFRDNTFDSAMAMFSLYHLPN
                     LHRGLDELHRVVKPLGRVAVATSSRLNKIIHRRDEVRIAEAKGASPPLRFTEKFPTEV
                     AEAVLPRFFRVVDRFDHRDVARFTGEVVDGRDGYDAYKASLDTMQPYFLKPSETPRGR
                     RKPAFETLQEWEDTLAAVVEPEFRRRFAKGEPFDDLIDRTIFILENPAD"
     gene            complement(7144..>7343)
                     /locus_tag="VFH39_00205"
     CDS             complement(7144..>7343)
                     /locus_tag="VFH39_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6924237.1"
                     /translation="RHHGEVHPQGIILKIPVTQQDIADMLSMTRETVTRQYARLKRNG
                     LIAMDRHLVITNLPGLQAAYA"
BASE COUNT         1336 a         2086 c         2302 g         1619 t
ORIGIN      
        1 caagcgcata gtaccagctt atagcctgcg gaccggttcg gccagaggca tgaatgattt
       61 acaaggcgat gctgttaggg tccgggccgg tcgggggctg ctgcgggggg gttgctggcg
      121 gtgtctgccc agctggagga gtcggcggtt ccgggggtgg cgtgaccacg gcgttggcgt
      181 ctggcatggc gaccggctcg ctggcggctt ctttggccag aagatcgctc aggtcctttt
      241 tgctgtcgac atcgttgagc ggctgtatga cttttttgcc tccagcggtg gtggagtccg
      301 gcttggccgg cgtggtagcg agttcgggct cggaggcagc ggcgggtgcc ggttcgggcg
      361 ggacttcgac ggccgtcacg tcggagggct tgccgggttc gctgccggta gtattcaacg
      421 acgccagggc gtcattcatg acctggtcgt tcgagctttg gtcatcagcc ggtgctggct
      481 gattgctcgg gcttgttgcc gacgctgccg gttcagtagc tggcgcaacc agttcggccg
      541 gaggagctgc gggagtagcg gctgcagggg ctggttcagc agaagccggc tccgcggcgg
      601 ctgtttgagg agttggcgtg ctgacgaacg attcgatctg cgcttccatg gcggcagttt
      661 cggcggcggt ggtagcagct gcggatgtat cgtcggccgg aggggccgct acgggggcag
      721 cctgcgaggc ggcgggttcc ggagctggcg ccgctgcaac cggttcggca ggagctggcg
      781 cgacaggttg tggagccggt tggaccggag ccggttcggg tgccgcggca gccggtgcgg
      841 cttcatcatc ggcttcggtg gcggccggcg cggccggctc gtcgtcgagt agctggtggg
      901 ctgtcttgac gatatcctcg agcgtcactt gtgacttgac gaggtacttg tcggcgccga
      961 gctggtcagc ccgggtgcgg tcttcggcct ggcccaaagc agtgagcata atgacctgca
     1021 cgtccttgag gccgtcggtg ttacgcaaga tgtcgagcat ttcgaagccg ctgatctttg
     1081 gcatcatgac gtccgaaata accaggtccg gtcgctcttt tttggcgacg accagggctt
     1141 cttcgccgtc gctggcgctg acgatgtcat agccttcggc ctgcaggcgg gcttcataga
     1201 tttcccggag gttgttatcg tcctcgacta acagtatctt tgccatgatt tttgctcgct
     1261 ttttacgtgt tataggttat gcttttccat cattgtacca tgctttcaac gcttacaacc
     1321 ccgtggcggt cgggatgggc gccagcggtg cgttggcggc ttcctcggtc tgtaattgtt
     1381 tggcttgctg gctgctgagt cgcggcaggt tgatgaagaa cgtcgtgccc ttagcaagct
     1441 cgctttcaca ccagatgcgc ccctgataca gctcgacgat tttgcggcag atgaacagcc
     1501 ccaagccggt gccaccgatg gtacgggtgg ccgagttatc gacccggtag aacttctgga
     1561 acaggtgcgg gatgtcctcg gccggaatac cgggacccgt gtcgcgaatg tatatctgta
     1621 ccacctggtc gttaccggtt atgccgaggc tgatcttgcc ttcgtcggtg tacttgacgg
     1681 cgttgtcgaa cagattggtg atgacctcgc ggatgcgatc tgggtcgacg tgcacgaagt
     1741 atagcggtag gacaaccttg gcgttggtgt tgacggcctg ggcggcggac ccgaagttga
     1801 agtccatgaa gagccccttc ttctcggcgg agaatttgag gtcctggacg agctggtcga
     1861 ggaaggcgcc catttcgacg acctgcgggt ggttggtcag gcggccgtcc tccgctttgg
     1921 cgctggtgag caggtcctgg aacagctggc cgaggtgctg agtgctggcg tgggcctttt
     1981 cgagataacc gcgggctttc atatcgatgg tcgaaacctt gtcgttcagg gccaggctca
     2041 agtagccttc gatggcggcg accggcgtgc gcatttcgtg gctggcggta ctgatgaagt
     2101 ccgcccggcg gctttcctct tgacgctctt cgctgacgtc gcggaagatg ccgaccacac
     2161 ccgtgacgtt ctgctggtcg tcgagcaatg gagaggcggc gaagctgagg gcgatgatcc
     2221 ggcccgattt ggtcagcagg ttggcgtcat tggcacggac gctcttgccg ttcgtcatgg
     2281 cggtggttat ggggacggcc gactcatcga tcggctcacc cttgtcgttg acgaacttga
     2341 agaccgatct ccagtcgaga ccgctggctt cttcgatggt ccagccggtg atggtggcgg
     2401 cgccggggtt gaagacatgg attgctccct ggtcatcgag cagtaccaca ccgtcctcga
     2461 tggcattcag aatgatgctg gacttaagct tttcgtcgcg caagcggtca gccaatttgt
     2521 cgctaccctg gttcgtacgt ttgaaccacg ccatcagttc ccaccgtcct gtgctgtcag
     2581 tcttgccata tcgttaatgt ttattatctg ttttttgagc cgtttttgca atcaccgcag
     2641 cgcctgatca tgccggcaat cgaatggcac gcatccggaa ggcgcggttt tcggggtggc
     2701 ccagggggtc cgtcgtcatg gctccccgct aaggtgccgg caggcgttgg aatataccta
     2761 cccgctactg gtagaaggca tcggtaaacc gcgggtcgtc ggcaaacccg ttgtaatgct
     2821 gctgctcgat cgggtcgaaa ccgttcaggc cgtactccac ccggttatcg ccggcggggg
     2881 tcggagcgcc gccgggcccg cccgccgaat cgtacaccat gtacagtttg atcttcggga
     2941 aggtgttggc gtccagggct tgcttgatgc tgtcccagta tttataggct gtcggttgat
     3001 caacattgct aatgctgaat tcagccacgc cccacggctt cgacaaatag tcgtgtgccg
     3061 cgtcgctggc gctcgtcagg aaccgatagg tggttccgac cgtgctttgc caggtgtcat
     3121 aatttttgct gccgtagcgg tcaaaatcga cccagtctat taaattgttg ccgggccaca
     3181 gatcatcgat catgcaggtg gccttcggac cgcccaggta gacgatcgac cagacgacgt
     3241 tcgttacacc gtcggccttg aagcggttct cgacattggc ccacatcgcc cggtactgcg
     3301 ccggtgtgcc gcccgtagcg ccgcttcggt aggccccgga actgcagccg gggacgctgc
     3361 cggcggtcac ttccagttgc ggttcgtgcc agagcgtcag gaagatcttg tgactgccta
     3421 gtgccttgat gctcgcggcc atgtgatcga tcgccagatt ggtcgtggca ttgctactgt
     3481 cggctgcctc ctgaaaggta gcggccggct tccaggagat aaacagcagc gtgttcgggc
     3541 gcgtcgcgaa agcttgctcg ccagtaatca gcgggtcgtc gaaattgtgg taattgccgc
     3601 gtaccacgtc gagctggcgg ccgatgcgtt tctcatggtc cagggtctgg ccggtcaggt
     3661 tggccaccac gggtggagga tagtgggcgg catgtccgcc cagccagggc ctacaggagt
     3721 taaccaaaat cggtgaaatc gtgcacttgt cgccgctagt gaacttgacc gcctcaccgc
     3781 cggacgcctt agcgtctttg acgctaacgg ccttgccgtt gagcgtaccg gcttcggcct
     3841 cgagagacgc ggtgtcggtc tgggcatggg taagcagcac gagcagcgaa ccgattaatg
     3901 cgatggtcac cacgatcacg gtctcggtgg ctcggcgctt cagctgttct cgacccaggc
     3961 gcattttttg cataagtatt acctagagtg tagcaatgac gtccagtctg tgccagcagc
     4021 agaaagattt gagtggaata caaaaactgt ccagatacca gcgctaatga aaaacaccca
     4081 cgcaaggtgg gtggctttca ttggtgaccc cgaggggagt cgaacccctg atcttcagga
     4141 tgagaacctg acgtcctgga ccactagacg acggggccaa accgcaggcg gaatgatttt
     4201 gtgcttgcat aaaatcttac gccgaggatt tggactagcg atacccgcag ggtatcgtgg
     4261 ggccaaactg tgtggcaaag gaaccatact tccacggttc ccgcttccaa aatggcagcc
     4321 gaacaatggt atcacatctg ttaccgtacg tccagacacc gctgcatcgc cgatgcgcta
     4381 tactatcgct atgccattca aaaacagaca tgttccgcat gacccggtct ggccggtcca
     4441 gctggctatg ctggtggcca tcgccctgca ggtcgccctg cccgacacgt tctcggcagc
     4501 ccgctacgcc ataccggccg ctgagctgct cctgcttatc gccctgtcct tcaccacccc
     4561 gaaggagcgc attttccgct cgatgagccg tcgtataaac gtcatcctgc ttatcgccct
     4621 gaccagcgcc gccaatgctt actcgctcgg cgtagtctgt aaccgtctgt tgcagggcgg
     4681 ccacgtatcc aacgggcggg agctcatcct gaccagcatc aacatcttcc tgaccaacat
     4741 catcatcttc ggcctgtggt actgggaaat ggatggtggc ggccccggcg aacggtcgca
     4801 catcgccaag tacgagcagg actttttatt cccgcagcac cataacgaag actacaagca
     4861 cccggactgg aagccgacgt ttctggatta tctgtatgtg agcagtacta acgccatgac
     4921 gttcggccca gccgatacca aaccgctgag ccggcgcgcc aagatgctaa tgctgttgca
     4981 gtcgacgata tccctcatcg tggtggccct agtggcggct cgtgccgtcg gcatcctgaa
     5041 ctagctattt gtggtatggc atgccggcgt tgatggtgct ggcacggtac agcgtctcga
     5101 gcaggatgat catcgccagg tcatggggaa acgttaaggg gccgaagctc cagcggtaat
     5161 cgcaggcctc gatgaccgca gacggcaagc cttccggacc gccgatgatg aaactgacct
     5221 cttgggccgc cagttcccgc tcccgcaaga aaccggccag ctgcgggctg gacatggtgg
     5281 cgccgtttat caccagggcg accttgtagc cggtagccgc ttccaggatg cgagcggcat
     5341 cgccggtgta tttgtctgcc agctggctga cgctgacctt atggaaccgg ctcaggcgtg
     5401 gcaggtactc ttcccagcct tcctgggcat agtgtagctt cggcttgcca atggtgacga
     5461 tgcgcagttt catagccgta ctataccaag gtcatgggcg gtgcgtatct gtttgccggg
     5521 cagcagttcg tgcgccgccc gggccacgcc cggcaaagct tcacgggcat agtcatcgat
     5581 gacaatggcg ccgccaggct gcatgcgcgg cagtacaagc ttgagggagt ccctgatact
     5641 ggtgtagaag tcaccgtcca ggaacgcaag cgcaatggtc tccggcacgt cagtggtggt
     5701 caggtcgttg aaccagcctt tgtgaatgac cggcggtcgc agaccggcct ttttgaactc
     5761 cgtaagaaac tgcttcttgc tgacgctcag ttccccggcc ttgaactgtt ccccggccgg
     5821 gctggcatcc gcggtcgttt tcggcggcag cccttcgaat gagtcgtaga catgcaattc
     5881 acggccgtca tcacccagcg cgtcaagcag ccggcggata aacaagctgg tcgtgccgat
     5941 ataacagccg aactccacca ccgcccccgg tgtcccctgc tcccctaccc gctcgagctc
     6001 ctcaagaatg acggctactt gcgctttgct gacctgatca gagataagcg gatggctcga
     6061 cagtaggtgt tcgatcttca ttggcagtat tatagcctca atctgtacta tgttcgcttc
     6121 tgaacatttg aggtagtcaa atagaaatat catcggtact atatatagta tggagaagca
     6181 accaggcgac gctacggggc gccccggctg gcagcaccca tctgctgatg agcgccgcca
     6241 ggcccgtctc tgggagaaag aatacggagg ctcacaggcc ggcctggtta atcggtggag
     6301 ttactatcgg catgccctcc acggtgaggt tatctactac tgggaagcat accggcggct
     6361 cgaagccgac ccatccggca gcttactgga cgtcggctgt tcagatggtg tcagtttgtc
     6421 cgtgatccgg gccttgcagg agcaaggcgg ccacagcggg ccaatagtcg gtgttgacct
     6481 gaagcgcgtg ctggtgggca accgcgcagc gaagaggctt acccgaatgt tctacgacaa
     6541 caatgtgctg cccatccaat tcgtggtggg atcaggtgat catctaccct tccgcgacaa
     6601 tacattcgat agcgctatgg ccatgttctc gctgtaccac ctgcctaacc tgcatcgggg
     6661 cctggacgag ctgcaccggg tcgtgaaacc cctgggccgc gttgcggtcg cgaccagcag
     6721 taggttgaat aagataatcc ataggcgcga cgaggtacgg attgcggagg ccaaaggcgc
     6781 gtcaccccct ctgcgcttca cagaaaaatt cccgacagag gtggccgagg cggtgctgcc
     6841 cagattcttc cgcgtggttg accggttcga tcaccgtgac gtagcccgct tcacagggga
     6901 ggttgttgat ggccgggacg gctacgatgc ctacaaggcg tccctggaca ctatgcagcc
     6961 ctacttcctg aagccatcgg aaacgccccg cggccgacgg aaacccgcct tcgagacgct
     7021 tcaagaatgg gaagacacat tggccgcggt cgtggagccc gaattcaggc gtcgcttcgc
     7081 caaaggcgag ccgttcgatg accttatcga ccggactatc ttcatcctgg aaaacccggc
     7141 cgactaggcg taggccgctt gcaggcccgg cagattggta atgaccagat gacgatccat
     7201 ggcgatcagg ccgttgcgct tgaggcgggc atattgccgg gtaacggttt cgcgggtcat
     7261 ggacagcata tcggcgatat cctgctgggt gacgggaatc ttcaggataa taccctgcgg
     7321 gtgcacttcg ccgtgatgcc ggg
//
LOCUS       DASSFD010000009         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1516343, whole genome shotgun sequence.
ACCESSION   DASSFD010000009 DASSFD010000000
VERSION     DASSFD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516343"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="rpsP"
                     /locus_tag="VFH39_00210"
     CDS             <1..430
                     /gene="rpsP"
                     /locus_tag="VFH39_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET6924238.1"
                     /translation="MQRTGRKGHAMFRVVVQDSRRTPTSGKVIANLGSYDPHAKTVKL
                     DKEKTQFYLDHGAQPSDRVAKLAAAEGIKLPKWVNQSAAKQSAIRNPEKLRRNRPAEE
                     APAEEPAAEAAEPAEEAEAPAETEAPAEAAAEAEAPAEEA"
     gene            518..967
                     /locus_tag="VFH39_00215"
     CDS             518..967
                     /locus_tag="VFH39_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET6924239.1"
                     /translation="MNNSIDQQFVEFIVKSLVGNPDAVIVERRIDEKGVLLELTVDPE
                     DLGRVIGKRGATAQSLRTLLRALGTKNDARYNLKIVDNGEPENGGGSSTRAPRDDAAS
                     DDTATTTNPQHDDLDDADAEGDAKAEPESDVIARARKELADLDDLDV"
     gene            complement(1188..1811)
                     /locus_tag="VFH39_00220"
     CDS             complement(1188..1811)
                     /locus_tag="VFH39_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924240.1"
                     /translation="MKKILMSTALLLTVGLNAPAAHAATTTSTKTTCKVTTAGTRNKT
                     GQGGQFTLNDNGTVTGQFKVEGDNCAVAMASWTAPSVDGKPYDEQKLFDSTTGTFSTG
                     VHQLTVKLPGCFYQIDLVPGTSATDSKGGPEYFTNGGRHVIGSLHGGSTSCTPAKPTT
                     TPPQPTTPAATTLPNTGMNPAGAGVAALVTGVLGSFFYRKRLLAREA"
     gene            1951..2601
                     /gene="trmD"
                     /locus_tag="VFH39_00225"
     CDS             1951..2601
                     /gene="trmD"
                     /locus_tag="VFH39_00225"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6924241.1"
                     /translation="MKIQIITLFPEMFKGVLGSSMLWKASDRGIVEYSIIDLRQFGLG
                     PRKTVDDTPYGGGDGMLLRVDTLVAAVEAAKAVDPSAAVILPTPRGKTFLQSDAKATA
                     ASGHGMIIICPRYEGYDERITQWVDKQYCIGNYVLTGGELPAMVIIDSVVRLLPGVLG
                     GETSTEIESFQEDDQNIEFPQYTRPEEFQGLKVPDVLLTGHHGAIAKWRAARSHKS"
     gene            complement(2598..>3220)
                     /locus_tag="VFH39_00230"
     CDS             complement(2598..>3220)
                     /locus_tag="VFH39_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924242.1"
                     /translation="WGVRRAIIAGRSAGGLGALELAATGKISQATLVYAAEPVGCQTF
                     ANGDGKRSYNQYLKLQKRLMAGDSKLPEGQRYFIHPRNDAKELPLRAQASRIASMVLL
                     NVAEAYYNESHWGQDVVADYATAISARGVPVHIDFADISMVLPADHAQKSSYLDRLSN
                     GAHRESGVSTREQPGTMHRSFDNRDFFARCLAAAVQQRTGIELPNA"
BASE COUNT          692 a          941 c          956 g          631 t
ORIGIN      
        1 catgcagcgt accggccgca agggccacgc catgttccgc gtcgtcgtac aagactcacg
       61 ccggacccca accagcggta aagtaatcgc caacttggga agttatgatc ctcacgccaa
      121 gaccgtcaag ctcgacaaag agaagaccca gttctacctg gaccacggtg cccagccgtc
      181 cgaccgcgtc gccaagctgg ccgccgccga aggcatcaaa ttgcccaagt gggtgaatca
      241 gagcgccgcc aagcagagcg ctatccgcaa tccggagaag ctgcgccgca accgtccggc
      301 cgaagaagct ccggcagagg aacctgcagc tgaagctgcc gagcccgccg aagaagctga
      361 agctcctgcc gagaccgaag ctcccgctga agccgctgca gaggccgaag cgcccgccga
      421 agaggcctaa gccgagcggc ccaaaagcaa gaatccttac aatccacccc ctgttttttg
      481 ctataatgtc agccataccg taagtggagt accggttatg aacaacagta ttgaccaaca
      541 gttcgtagag ttcattgtca aatctctggt gggcaatccg gacgcagtta tcgtcgagcg
      601 acgcatagat gaaaaaggtg tgttacttga gttgactgtc gacccggagg atctgggccg
      661 cgtcatcggc aagcgcggtg cgacggcgca gagcctgcgt acgcttttgc gcgcgctcgg
      721 caccaagaac gatgcccgct ataacctgaa gatcgttgac aatggcgagc ccgaaaacgg
      781 cggtggctcg agcacccggg cgccgcgtga cgacgcggct tcggacgaca ctgccacgac
      841 caccaacccg cagcacgacg atcttgacga cgccgatgcc gaaggcgacg ccaaggccga
      901 gcccgaaagt gacgtcatcg cccgcgcccg caaagagctg gctgacctcg atgacctcga
      961 cgtgtaaact ttccacaacc gaagtaaaaa agcctgcgat ttacccttgc atagcgccgg
     1021 acagtcgctt atacttacca tcagtctgag atagacggta catcaaaaaa caaaaatatc
     1081 gaagctcatt gcgagccaat ttggcactag tcggattggg agctttatgt gacaaaaaga
     1141 gccttcttcg gaaggctctt tttggttgcg gtgcctgcgt gcccggatta ggcttcgcgg
     1201 gccaacaggc gcttgcggta gaagaagcta ccgagcacgc cggtaaccag tgcggccacg
     1261 ccggctccgg ccgggttcat accggtgttg ggaagcgtcg ttgctgccgg tgtggtcggt
     1321 tgcggaggcg tcgtggtcgg cttcgcaggc gtacaactgg tgctgccgcc gtgcagggaa
     1381 ccgatgacat gtcggccgcc gttcgtgaag tactcggggc cgcccttgct gtcggtggcg
     1441 ctggtccccg gcaccaggtc gatctggtaa aagcagccag gcagtttgac ggtcagctgg
     1501 tgaacgccgg tcgagaaagt gccggtggtg ctgtcgaaca acttctgctc atcgtacggc
     1561 ttgccatcca ccgaaggggc cgtccagctt gccatcgcga cggcacaatt gtcgccctcg
     1621 accttgaact ggccggtgac cgtgccattg tcgttgaggg tgaactggcc gccttggccg
     1681 gtcttgttgc gcgtcccggc agtcgttacc ttacaagtcg tcttggttga cgtagtggta
     1741 gccgcgtgcg ccgcaggcgc attcaggccg acggtcagca acagggcggt actcattaag
     1801 attttcttca tgatctccac tccttaaaga atattgatat tatacacaaa aaatacaaaa
     1861 tgtcaatata accccctgct cgagtgtcag tgctgcctcg ctcgcagcag aaaaggatgt
     1921 cctagcaagc gggctgttac aatataagca atgaaaattc aaatcatcac gttgtttccg
     1981 gagatgttca aaggcgtgct cggcagctcc atgctctgga aggcgagcga ccggggcatc
     2041 gtcgagtatt ccatcatcga tctgcggcag ttcggcctcg gaccgcgcaa gacggtggac
     2101 gacacgcctt acggtggtgg cgacggtatg ctgctacggg tagatacgct tgtggccgcc
     2161 gtagaggccg ctaaagccgt cgatccaagc gcagccgtca ttctgcctac tccccggggt
     2221 aaaaccttct tgcagtccga tgcgaaggcc accgcggcat ccgggcacgg tatgatcatc
     2281 atttgccccc gctacgaagg ctacgacgaa cggattaccc aatgggtgga taagcagtac
     2341 tgtatcggca attacgtgct gacggggggc gagctgccgg ccatggtcat catcgacagc
     2401 gtggtgcgcc tgctgcccgg cgtcttgggt ggtgagacca gcaccgagat cgaatcgttc
     2461 caggaggacg accagaacat cgagttcccg cagtacacgc gccccgaaga gttccagggt
     2521 ctaaaagtcc cggatgtctt actcaccggc caccacggcg caatcgccaa atggcgcgcc
     2581 gcacgtagcc ataagagtta ggcgttcggg agttcgatgc cggtgcgctg ctgcacggca
     2641 gccgccaggc agcgggcgaa aaagtctcgg ttgtcgaatg aacggtgcat ggttccgggc
     2701 tgctcgcggg tactgacacc ggattcccga tgggcgccat tgcttagccg gtcaaggtaa
     2761 ctactctttt gtgcatggtc tgccggcagt accatgctga tgtctgcgaa atcaatgtgg
     2821 accggaactc ctcgggcgct gatagcggtc gcataatctg caacgacatc ctgcccccag
     2881 tgggattcgt tgtagtacgc ttcggccaca ttgaggagca ccatcgatgc gatacgactg
     2941 gcttgcgccc gaagcggcag ttctttggcg tcattgcgcg gatggatgaa ataccgctgt
     3001 ccttcaggca gtttgctgtc gccggccata agccgtttct gtagtttaag atactgatta
     3061 tagctgcgct taccgtcgcc gttcgcaaac gtctggcagc cgacgggttc ggcggcatat
     3121 accagggtgg cctgtgagat ttttccggtc gctgcgagtt ctagtgcgcc caggccgcca
     3181 gccgaacggc cggcgatgat tgcccgccgt acgccccata
//
LOCUS       DASSFD010000010         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1015027, whole genome shotgun sequence.
ACCESSION   DASSFD010000010 DASSFD010000000
VERSION     DASSFD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015027"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..823
                     /locus_tag="VFH39_00235"
     CDS             113..823
                     /locus_tag="VFH39_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6924243.1"
                     /translation="MDEFFVGASEVEYSRNESIDVGGVFCRLKSGYVGLFSGQERSKR
                     LLFIYKPGDLFPFTMHSNIFPKRPYSYRALSRVTLETAPVGGYDGKHFSRKALIEALD
                     YMQSILQMQFERIDNLQKHQVQRRLLERLYFSAKRLGVKKGDRVSIDVPMSHVDLASS
                     IGTSRETVNRLMKQLEACQILKVRRHRITIMSFKKLEAALHAKRIPEGPSRQPLPIIA
                     LQAGIACGAVLGLLQRMS"
     gene            complement(882..1820)
                     /locus_tag="VFH39_00240"
     CDS             complement(882..1820)
                     /locus_tag="VFH39_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6924244.1"
                     /translation="MKRNDVRKLTVRSRWLFLVAGLVVLYVVVPRLGGLKQSIGSLVN
                     AKPMWLAVALLATFVTYVAATWMYLLLAQHRLRGTTTFVVQLAGNFTNKLLPAGIGTA
                     GINYEYLRRQRHSRSGAAAVVTTNNTLGFVGNFVLILGLVTFTPARLSDLHWPAIGSR
                     AWLAAGLVVLLSMVIALIVLRGKLREAVVAFGASFWRYRQQPQRLVLALMVSMLLTTA
                     NAAVLYAAAHGIGLHVGMTAALIIMTIGVAGATVTPTPGGLGGAEAGLVAGFMLYGAS
                     SSTALAAALIFRLFTFWLPLLWGGSAFILGRSRNYY"
     gene            1910..2365
                     /locus_tag="VFH39_00245"
     CDS             1910..2365
                     /locus_tag="VFH39_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6924245.1"
                     /translation="MQRNELIDRIYENMGALKRGMLSSLQSIGGTPHLSGSQLELAFT
                     IKHLQPVGVKQLATQLRLTPGAVSQVADALVQTGLVTRQHDETDRRKSYLSLTAEGDR
                     VLHRVHQDRQALFRSVMEDMTDQELTEWLHIQQKLIDHLATITNRNTKE"
     gene            2368..>2609
                     /locus_tag="VFH39_00250"
     CDS             2368..>2609
                     /locus_tag="VFH39_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924246.1"
                     /translation="MLFRTKATKTPRPVVRATEAAAEASISQPVRSHAEIMVVITALM
                     LAMLLAALDQTIVSTALPKIASDLHGLSKYSWVATS"
BASE COUNT          618 a          794 c          703 g          494 t
ORIGIN      
        1 attacctggg gattgtcatg tcaagcattt ctcatcacac ttcacgggca ctccctgcca
       61 gcaaccacct gcaacctgac ggtaccgttc cggcatatga taaggtggat gtatggatga
      121 gtttttcgtg ggagcgtccg aggtcgaata cagccgcaat gaatcgatag atgtgggtgg
      181 tgttttttgc cgcttgaaaa gcggatatgt cggcctgttc agcggccagg agcgatccaa
      241 gcgcctgctg ttcatatata agccgggcga cctattccct ttcactatgc actccaacat
      301 cttccctaag cgcccataca gctatcgggc tctgagccga gtcacgctcg aaacagcacc
      361 ggtcggcggt tatgacggca aacatttcag ccgaaaagca ctgattgaag cactcgacta
      421 catgcaatcc atcctgcaga tgcagttcga gcgcatcgat aacctgcaga aacaccaagt
      481 gcagcgccga ctgctcgaac ggctttattt ttccgccaag cgtctgggag taaaaaaagg
      541 cgaccgtgtc agcatagacg tgccgatgtc gcatgtcgat ctggcatcgt ccatcggcac
      601 cagtcgtgaa actgtgaacc ggctgatgaa acagctggaa gcatgccaga ttctcaaggt
      661 ccgacggcac cgtattacca tcatgtcgtt caagaagctg gaggcggcgc tgcatgccaa
      721 acgtattccg gaggggccgt cccgccagcc gctgcccatc atcgctctgc aagctggaat
      781 tgcctgcggt gcggtgctcg ggctgttgca acgcatgagc taatgacccg tcattggtca
      841 gctgccacgc ggatacaggg ctgccccagc agcctcacct gctagtaata gttccggctt
      901 cgcccaagga tgaacgcact accgccccat aataacggca gccagaacgt gaaaagtcga
      961 aagatcagtg cggcagccaa ggcggtggaa ctcgaggcgc catacagcat gaaaccggcc
     1021 actaggccgg cctccgcccc gcccagcccg cctggtgtcg gtgtaaccgt cgctcctgcc
     1081 acgccaatgg tcatgataat cagcgcagcg gtcatcccga cgtgcaaacc gataccgtgg
     1141 gctgcggcat acagtaccgc cgcattggcg gtagtcagca gcatcgatac cataagcgcc
     1201 agtacgagcc gctgcggctg ttggcgatag cgccagaaac tagccccgaa ggccaccact
     1261 gcctcacgta gctttccccg caggacgatc agggcgataa ccatcgacag cagcacgacc
     1321 aggccggctg ccaaccaggc cctggagccg attgccggcc agtgcagatc gctcaaccgg
     1381 gccggcgtga acgttaccag ccccaggatc agtacgaagt taccgacgaa tcccaaggta
     1441 ttgttggtcg taactacggc ggccgcgccg ctccggctgt gtcgctgccg ccgcagatat
     1501 tcataattga ttccggccgt gccgatgccc gccggcaaca atttgttggt gaagttgccg
     1561 gctagctgca ctacgaacgt ggtcgttccg cgcaagcgat gctgggccag taacaggtac
     1621 atccaggtcg cagccacgta cgtcacgaac gtggctagta gagccaccgc caaccacatc
     1681 ggcttggcat tcaccaggga gccgatgctc tgcttcaggc ctccgagccg cggcaccacc
     1741 acgtacagta cgaccaggcc ggccaccagg aacaaccaac gactgcgtac cgtcagcttg
     1801 cggacatcgt tgcgcttcat gtggtttcat cataacagtt atgcttaggg cgcgcgataa
     1861 gactggttga catattagtt tagtaactat acaatttaat acgtaaacta tgcagcgaaa
     1921 cgaattgatc gaccgcatct atgagaacat gggcgccctt aaacgcggca tgctcagcag
     1981 tttgcagtct atcggcggaa cgcctcacct gtcgggcagc caactggaac ttgccttcac
     2041 catcaagcat ctgcaaccag tcggcgtcaa acagctggcg acacaacttc gtctgacgcc
     2101 gggcgccgta tcgcaggtag ccgatgcact ggtacagacc ggcctggtca cgcggcagca
     2161 cgacgaaacc gaccgccgca aatcgtacct gagtctgaca gccgaaggcg accgggtgct
     2221 acaccgggtc caccaggacc ggcaggcact tttccgcagt gtcatggaag acatgaccga
     2281 ccaggagcta accgagtggt tacatatcca acagaaactt atcgaccatt tggctaccat
     2341 aacaaaccgt aatacaaagg agtaaaaatg ttatttagaa ccaaggcgac caaaacgcct
     2401 agacctgtcg tgcgcgccac cgaggcggcg gccgaagctt ccatcagcca gccagtccgc
     2461 tcgcatgccg aaatcatggt ggtcatcacc gccctcatgc tggccatgtt actggcggcg
     2521 ctcgaccaaa ccatcgtaag cacggcgctg cccaagatag ccagcgacct gcacggactc
     2581 agtaaatact cgtgggtggc gacgtctta
//
LOCUS       DASSFD010000011         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1263403, whole genome shotgun sequence.
ACCESSION   DASSFD010000011 DASSFD010000000
VERSION     DASSFD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263403"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2320
                     /locus_tag="VFH39_00255"
     CDS             <1..2320
                     /locus_tag="VFH39_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET6924247.1"
                     /translation="QAIVSNMVASFSLFDAPGRNGFKYSPAKDIESFVTDYLLRFFNP
                     AGKPNILNAVELATLFHFPDQKNIPTSQVTRQDSKQVDGPRNVPETGLLLGYNVFRGV
                     KKPIRLDLMDRQRHMYVVGQTGVGKSVFLENLAFQDMLAGNGFAFVDPHGDTAEKLLS
                     MIPKERTEDVIYFSPADMEYPLGLNLFEFNNPDQKDFLIQEALNMLYKLYDPQHQGIM
                     GPRYEHLFRMAALTVMADPEGGTFIDIPKLFRDPKYVGQKLKYITDQNVVEFWKKEMP
                     QSQRSNEFGEVVSWFVSKFGAFLSNEMMRNIIGQNKSAFNLRDVMDNKKILLVNLSKG
                     RLGDLNSKLLGMIFVMKFQAAAMSRADIPEDQRVDFSLYVDEFQNFSTDSFATIMSEA
                     RKYHLNLIVANQFTTQLTPEIRDAVFGNMGTIVSFRIGQNDVEAIAKYFQPTFDADDL
                     LRVPNYNTIMRTLINGVPTQPFSMATLPPLGSPNPKLADALKQLSAAKYGRPRANVEK
                     EIFARLATKQEAPAPRPGGYGAPGAGAFGPSPSPFGSPAPAAGSFGAPASPYAPPGGS
                     WQAPAPAQPGPTGTGSFLDEWLTKRQTQPRRVPAPQPAAAAAPAPAAPMSAVSAPQGP
                     IVPPAAEPSMPVITQHHTPMMQPRQDDRSADQLGADMEPEASAGDAPAESSASDVLKP
                     SEDAIRQLGGETRIVSADGTQNISSSELSGDEAAKIAEELTADLKQSSAHKADAAKPE
                     SAAGPADKPTDRPPEEDTIAIDQDGTLHIKNQ"
     gene            2455..>4409
                     /gene="gyrB"
                     /locus_tag="VFH39_00260"
     CDS             2455..>4409
                     /gene="gyrB"
                     /locus_tag="VFH39_00260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET6924248.1"
                     /translation="MATDKSPKSSYSADQIQVLEGLEPVRKRPGMYIGGTGIEGLHHL
                     VWEIVDNGIDEALAGHATEVAVTLLADGGVRVWDNGRGIPTDIHPKTGKSTVETVLTV
                     LHAGGKFGGGGYKVSGGLHGVGSSVVNALSDKLHVKIHRDGKIHEQDYVRGVPQGDLK
                     VTGKTTITGTEITFWPDASIFTESQEFNYQTILDRLRHAAYLTKGVHTNLQDERTGER
                     YGFYFEGGIRSYVQHLNHGKEPVDEDIFYVDKPVQDCNVEVALQYTDAYTETLKAFAN
                     NVLNPEGGTHLTGFRAALTRVINDYARKSGLIKEKEENLTGEDTREGLTCVISVKLPD
                     PQFEGQTKGKLGNPEMRGYVEQVMNEYFAYYLEEHPNVARKIVGKALLAARARKAARA
                     ARDNILRKGVLDGASMPGKLADCSSKDPKSSEIYLVEGDSAGGSAKTGRDSKTQAILP
                     LRGKVLNVERARLDKMLANNEILSLIKALGVGIEESFDLGGLRYDRIIIMTDADVDGS
                     HISTLLLTFFFRFMQPVIDGGHVYLAKPPLFELVRPGRKNPVFIYDESELDKVIDAEI
                     ERRKKEGLKVNKDDERYKQAGFIEQKRYKGLGEMDAEQLFETTMNPEKRVLVQVKVED
                     AEKADAIFNKLMGTEVELRKNFIQANA"
BASE COUNT         1016 a         1327 c         1311 g          755 t
ORIGIN      
        1 gcaagccatc gtcagcaaca tggtggccag tttttcgttg ttcgatgcac ccggccggaa
       61 cggcttcaaa tacagccccg ccaaggacat tgaaagcttc gtgaccgact acctgctgcg
      121 gttcttcaac ccggccggca agcccaatat tctgaacgcg gtggagctcg ccacgctctt
      181 ccattttccg gaccaaaaga acattccgac cagccaggtc acccgccagg attccaagca
      241 agtggacggt ccgcgcaacg tcccggagac gggattgctg ctgggctaca acgtgttccg
      301 gggcgtcaag aaacccatcc gcctggacct catggaccgg cagcgtcata tgtatgtcgt
      361 ggggcagacg ggcgtcggta agtcggtctt tttggagaac ctggccttcc aggacatgct
      421 ggccggcaac ggcttcgcct ttgtagaccc gcacggcgac actgccgaaa agctgctgag
      481 catgatcccc aaggagcgca ccgaggatgt catttacttc agcccggccg acatggagta
      541 cccgctgggc ctgaacctgt tcgagttcaa taacccggac cagaaggatt tcctgatcca
      601 ggaagccttg aacatgctgt acaagctgta tgacccgcaa caccagggca tcatggggcc
      661 gcgctacgaa cacctgttcc gcatggcggc gctgacggtc atggccgacc cggaaggcgg
      721 caccttcatc gacatcccga agctgttccg cgaccccaaa tacgtcggcc agaagcttaa
      781 gtacatcacc gaccagaacg tggttgagtt ctggaaaaaa gagatgccgc aatcacaacg
      841 ctcgaacgaa ttcggcgaag tggtgagctg gttcgtcagt aagttcggcg ccttcctgag
      901 taacgaaatg atgcgcaata taatcgggca gaacaaatcc gcgttcaacc tgcgcgacgt
      961 gatggataat aaaaagatac tgctggtgaa cctgagtaaa ggccgcttgg gcgacctgaa
     1021 ctccaagctg ctaggcatga tcttcgtcat gaagttccag gcggcggcca tgagccgggc
     1081 cgacattccg gaagaccagc gggtggactt cagcctgtac gtcgacgagt tccagaactt
     1141 ctcgactgat tcgttcgcca caatcatgtc tgaagcccgt aagtatcacc tgaacctgat
     1201 cgtggccaac cagttcacga cgcagctgac gccggagatc cgcgacgccg tgttcggtaa
     1261 catgggtacg atagtcagtt ttcgtatcgg tcagaacgac gtcgaggcga ttgccaaata
     1321 cttccagccg acgttcgatg ccgatgacct gctgcgcgtg cccaactaca acaccattat
     1381 gcgtacgctt ataaacggcg taccgacgca gccgttcagt atggcgacgc tgccgccgct
     1441 cggcagcccg aatccgaagt tggccgacgc cctcaagcag ctgagtgctg ccaaatacgg
     1501 ccggccgcgc gccaatgtgg agaaagagat ttttgcccgc ttagccacca agcaggaagc
     1561 accggcacca cggccgggag gttacggggc gccaggggca ggggcgttcg gtccgagccc
     1621 cagcccgttc ggctcgccgg caccggccgc cggcagcttc ggtgccccgg catcgccgta
     1681 cgctccgcct ggcggttcgt ggcaagcgcc ggcaccagcc cagccgggcc cgaccggaac
     1741 cggctcgttc ctggatgagt ggctgaccaa gcgccagacg cagccgcgac gcgtgccggc
     1801 cccgcagccg gcggctgccg ctgcgcctgc tccggcggcg cccatgagcg ccgtaagcgc
     1861 cccgcaggga ccgatcgtac cgcccgcggc tgagccgagc atgccggtca taacgcagca
     1921 ccatacgccc atgatgcaac cgcgccagga tgaccgatcc gccgaccaac tgggcgccga
     1981 tatggagccc gaagcgtcgg caggcgatgc tccggctgag tcgtccgcca gcgacgtgct
     2041 gaagccctca gaagacgcca taaggcaatt aggcggcgag acccggattg tgtcggcaga
     2101 cggcacgcaa aatatctcca gcagcgaact gagcggcgat gaagcggcca agatcgccga
     2161 ggagctgacc gccgacctga agcagtcatc cgcccacaag gcagacgctg cgaaacccga
     2221 atccgctgcc ggtcctgcag acaagccgac cgaccggccg ccggaagagg acactatcgc
     2281 catcgatcag gacggcacgc tgcacatcaa gaaccaatag cctctgggta acagaggcgc
     2341 caaggtgttg gcataagcgt taatttacgc tatactatat ataagtagtg ggaacgggct
     2401 accgggtgtc ggtagtcggc acactgaatg cttaagccaa gagggttatt agaagtggca
     2461 acagacaaat ctccgaaatc aagttattca gcagatcaga tccaggtgct cgaaggcctg
     2521 gagccggtgc gcaagcgccc gggtatgtac atcggtggaa ccggtataga aggcctacac
     2581 cacttggtgt gggaaatcgt cgacaacggt atcgacgaag ccttggctgg tcacgcgacc
     2641 gaagtggcgg tgacgctgct ggccgacggt ggtgtccgcg tctgggacaa cggccgcggt
     2701 attccgaccg acattcaccc caagacgggc aagagcaccg tcgagacggt gctgacagtg
     2761 ctgcacgccg gcggtaagtt cggcggcggc ggttacaagg tctccggcgg tctgcacggc
     2821 gtcggcagta gcgtggtgaa cgcgctgagc gacaagctgc atgtgaagat ccaccgcgac
     2881 ggcaagattc acgagcagga ttacgtgcgc ggcgtaccgc agggcgacct gaaggtcacc
     2941 ggcaagacca ccatcaccgg caccgagatc accttctggc cggacgccag catctttacc
     3001 gagagccagg agttcaatta tcagactatc ttggaccggc tgcgccatgc tgcctacctg
     3061 accaagggtg tgcataccaa cctccaggac gagcgtaccg gtgaacgcta cggcttttac
     3121 ttcgagggcg gtatccgttc ttacgtccag cacctcaatc acggcaagga acccgtcgac
     3181 gaggatatct tttacgtcga caagccggta caggattgca acgtcgaagt ggcgctgcag
     3241 tacaccgacg cttataccga gacgctcaaa gccttcgcca acaacgtgct caaccctgaa
     3301 ggtggtacgc acctgaccgg tttccgcgcc gcgctgacgc gcgtcatcaa cgattacgcg
     3361 cgcaagagcg gacttataaa ggaaaaggaa gagaacctga ccggtgagga tacccgcgag
     3421 ggcctgacct gcgtcatttc cgtcaaactg ccggacccgc agttcgaagg ccagactaaa
     3481 ggcaagcttg gtaaccccga aatgcgtggg tatgtggagc aggtgatgaa cgaatacttc
     3541 gcctactacc tggaagaaca cccgaacgtt gcccgcaaaa ttgtcggcaa ggcgctgctg
     3601 gccgcccgcg cccgcaaagc cgcccgtgcc gcccgcgaca acattctgcg caaaggcgtg
     3661 ctcgatggtg caagtatgcc cggcaaactg gccgactgta gcagcaagga cccgaagagt
     3721 agcgaaatct acctggtgga gggcgattcc gccggcggtt cggctaagac cggccgcgat
     3781 agcaagacgc aggccatcct gccgctgcgc ggtaaggtgc tgaacgtcga gcgtgcccgc
     3841 ctggacaaga tgttggccaa taacgagatc ctgagcctca ttaaggcact tggtgtcggt
     3901 atcgaggaat cattcgacct tggtggcctg cgctacgacc gcatcatcat catgaccgac
     3961 gccgatgtcg acggcagcca catttcgacg ctgctgctga cgttcttctt ccgtttcatg
     4021 cagccggtca tcgacggcgg ccatgtgtat ctggctaagc cgccgctgtt cgaactggtc
     4081 cgtccgggcc gcaagaaccc ggtattcatt tacgacgagt ccgagctcga caaggtcatc
     4141 gacgccgaga tagagcgccg caagaaagaa gggctgaagg tcaacaagga cgacgaacgc
     4201 tacaagcagg ccggcttcat cgagcagaag cgctacaagg gcttgggcga aatggacgcc
     4261 gagcagctgt tcgagacaac catgaacccg gagaaacggg tgctggtaca agtcaaagta
     4321 gaagatgctg aaaaagccga cgctatcttt aataagctga tgggcaccga agttgaactg
     4381 cgtaagaact tcattcaggc gaacgccaa
//
LOCUS       DASSFD010000012         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_367727, whole genome shotgun sequence.
ACCESSION   DASSFD010000012 DASSFD010000000
VERSION     DASSFD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367727"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFH39_00265"
     CDS             <1..663
                     /locus_tag="VFH39_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET6924249.1"
                     /translation="VISGGKFGQFQLQLLENLHAGPTELQNLHLMPTCGTQYYRYDAS
                     AKSWQREYAELIDNKDKQRIIDALNKGFDDLGYRAKKVYGPEVEDRESQITFSVLGQD
                     IVDVLGEEGVKQKYAWDPDNSKKLAVREYVAPLIPDFEVRVGGVTSIDVTRPGIDKAY
                     GVQKLMDMLEVGKDDVLFLGDRLQEGGNDYPVKAMGVDCLEVSRWEDTALVLEGILHV
                     VS"
     gene            complement(660..1190)
                     /locus_tag="VFH39_00270"
     CDS             complement(660..1190)
                     /locus_tag="VFH39_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924250.1"
                     /translation="MGRTRRSIEGRLNTWAFVVLVAVSGIGLAVWRLPNMNFVSVHTS
                     VDTFLHYGDAVMTRPTGLAQLHTGDLVSYYDPLQPGNMRTSRVQAVHAAGGSFVPAGA
                     EISQHPADVSASLLIGKVHYRLSYAGYLLDFVRSRLGLVLGVYLPALIILNHEILLLA
                     ATYRRQHYRLPVAAND"
     gene            complement(1203..1799)
                     /locus_tag="VFH39_00275"
     CDS             complement(1203..1799)
                     /locus_tag="VFH39_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924251.1"
                     /translation="MKKFLSKHSPILRAFAVVSLVGATVTTMTYATLQSPTASLTSNN
                     IKSATANLVIGTASPTSTAYNATHTGFTFNNVIPGGPAVPDGGNTFYLKNTGSTTLKL
                     KLNISGTPTNPGNVDLSKVNVTVTRTDTGASQTASLQALLDTSDTGGLALTDTLAPGS
                     TGTGYTITVGMDADAYTGSGNDAAIGPFDFVFSGTVDA"
     gene            2135..2302
                     /locus_tag="VFH39_00280"
     CDS             2135..2302
                     /locus_tag="VFH39_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924252.1"
                     /translation="MAKEVDKEQLQTYENSLKYWRSWGSWFSWGSPVGLGLFFIAVAF
                     TVWLLGQTFNW"
     gene            complement(2367..>2878)
                     /locus_tag="VFH39_00285"
     CDS             complement(2367..>2878)
                     /locus_tag="VFH39_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET6924253.1"
                     /translation="LKDPDLIALAWIVDFPFYELDEKTKKLDFSHNPFSMPKGGMEAL
                     ESEDKLRIVADQYDMVMNGYEICSGGVRNHNPEVMYKVFGILGFDQQYVDARFGAMIN
                     AFKYGAPPHAGCAFGIDRMFMVLVGEDNIREVVAFPKNGSGVDAMMQSPNTVEIEQLD
                     DLNLRIVEG"
BASE COUNT          618 a          777 c          885 g          598 t
ORIGIN      
        1 gtcatatccg gcggtaagtt cggccagttc caactccagc tgctcgagaa cctgcatgcc
       61 ggaccgaccg aactgcagaa cttgcatctg atgcccacct gcggcacgca gtactaccgc
      121 tacgatgcgt cagccaagag ctggcagcgg gaatatgccg aactgattga caacaaggac
      181 aagcagcgca tcatcgacgc gcttaacaaa ggcttcgatg acctcggcta ccgcgccaaa
      241 aaagtctacg ggccggaggt tgaggaccgc gaaagccaga tcacgttctc ggtactgggc
      301 caggacattg ttgatgtact cggcgaagaa ggtgtgaagc agaagtatgc gtgggatccg
      361 gacaacagca agaagctggc ggtacgcgag tacgtggcgc cgcttattcc ggatttcgag
      421 gtccgcgtcg gcggcgtaac ctccatagat gtaacccggc cgggtatcga caaagcctac
      481 ggcgtccaga agctgatgga catgctcgaa gtcggcaagg acgacgtcct gttcctgggc
      541 gatcgcttgc aggaaggcgg taacgattac ccggtgaagg ccatgggtgt cgactgcctg
      601 gaagtctcgc gctgggaaga cactgccctg gtgcttgaag gcatcctgca cgtcgtcagc
      661 tagtcattgg cggctaccgg cagacggtag tgctggcgcc ggtaggtggc ggccagcagt
      721 aggatttcgt gattgaggat gatgagcgcc ggcaggtaga cgccgagcac cagtcctaag
      781 cgactgcgga cgaagtcgag taggtacccg gcgtagctta gccggtagtg cactttgccg
      841 atcagcagcg aggcgctgac gtcagccgga tgttggctta tctccgcgcc ggcaggcacg
      901 aatgaaccgc cggctgcatg gactgcctgc acgcggctgg tacgcatatt gcccggctgc
      961 agcggatcgt aataactcac caggtcgcca gtatgcagtt gtgccaagcc cgtcggccgc
     1021 gtcatcactg catcgccgta gtggaggaat gtatcgacgg acgtatgcac actcacgaag
     1081 ttcatattcg gcaggcgcca taccgccaag ccgatgccac tgacggcaac cagcacgaca
     1141 aaggcccagg tattcagccg accttcgata ctgcgacgcg tgcggcccat gtaatcttct
     1201 cgctaggcgt cgacggtgcc gctgaagacg aagtcgaacg gcccgatggc tgcatcgtta
     1261 ccgctgccgg tgtaggcatc ggcgtccatg ccgacggtta tggtgtagcc cgtgccggtg
     1321 ctgcctggtg ctagtgtgtc cgtcagcgcc aagccgccgg tgtctgaggt gtccaggagg
     1381 gcctgcaggc tggccgtttg cgacgcgccg gtgtcggtgc gggtaacggt aacgttaact
     1441 ttgctaaggt caacatttcc agggttagtc ggtgtgccgc taatattcag cttcaacttc
     1501 aacgttgtgc tgccggtgtt cttcaggtag aaagtattgc cgccgtccgg tactgccggg
     1561 ccgccgggaa tgacattatt gaatgtaaaa ccggtgtgcg tcgcattgta ggccgtagag
     1621 gtcggtgagg cagtaccgat aacgaggttg gcggtcgcgc tctttatatt gttgcttgtt
     1681 aagctcgccg ttggactctg cagggtggcg tacgtcatgg tggtgacggt ggcgccgacc
     1741 aggctgacga ccgcgaaggc acgcaaaatc ggactgtgtt tgctaaggaa cttcttcatg
     1801 acgtatctcc tccgtcacgt taggtaatgt ggcgcagcct acgcctagcg aagcggcaag
     1861 tcaatactaa aatgtaattt gttagcttta gcaatattat gataaaacat gagcaataag
     1921 cagagcgggg gagttgccga agtgcggatg tggtgcttaa ataagcgtaa cgtggcagtc
     1981 acgaaccgct atgcgcctcc ggtcggtcca ggcgaaccac atagcccaga caatggagag
     2041 ccaccggacc ggctgcatga ctgttcccgg ccaggtcgaa ccgcgagccc gaatgcacac
     2101 ttagcaagta aagtatttat aagcagaggt aattatggca aaagaagtcg ataaagagca
     2161 gctgcaaacc tacgaaaaca gcctgaaata ctggcgttcg tggggctcct ggttcagctg
     2221 gggctcgccg gtcgggctcg gcctgttctt catagcagtc gccttcaccg tctggctgct
     2281 cggccagacg ttcaactggt agcagaaaac gcgccagcaa aaacagcccg ggccaaaccg
     2341 ggctgttttt atgatgcgtg agctaactac ccttcgacga tgcggaggtt gaggtcgtcg
     2401 agctgttcga tctcgacggt gttcggagat tgcatcatgg cgtcgacgcc gctgccgttc
     2461 ttggggaaag cgaccacttc gcggatgttg tcctcgccta ccagcaccat gaacatgcgg
     2521 tcgataccaa aagcgcagcc ggcgtgcggc ggtgccccgt acttgaaggc gttgatcatg
     2581 gcgccgaaac gagcatcgac gtactgctgg tcgaagccca gaatgccgaa gaccttgtac
     2641 atgacctccg ggttgtggtt acggacacca ccgctgcaga tttcgtagcc gttcatgacc
     2701 atgtcgtatt ggtcggccac aatccttagt ttgtcctcgc tctccagtgc ctccatgccg
     2761 cccttgggca tgctgaatgg gttgtgggaa aagtccagct tcttggtttt ctcgtccagt
     2821 tcgtagaacg gaaagtcgac aatccacgcc agggcgataa ggtcagggtc cttgaggt
//
LOCUS       DASSFD010000013         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1766278, whole genome shotgun sequence.
ACCESSION   DASSFD010000013 DASSFD010000000
VERSION     DASSFD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766278"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFH39_00290"
     CDS             <1..246
                     /locus_tag="VFH39_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924254.1"
                     /translation="VRNDGLFAVTHIDSAGELVETQVGDDATIDWTHVVPVGNDILFV
                     RNDGLFAVAHIDSAGELVETQVGDDATIDWTHVIAVG"
     gene            complement(258..1232)
                     /locus_tag="VFH39_00295"
     CDS             complement(258..1232)
                     /locus_tag="VFH39_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6924255.1"
                     /translation="MLRQDVPNIRRIRRGMISRFSEQTIAPWRKEVEGVADDLISKLR
                     DVHRAPDLNKSYFEPFIVRAVALSAGITEDESDKLYGFSNRVLVRVESADDRARICAA
                     WNELYEYSSELIQGRLDNPDDRLLSKIFAEFKKTRLCHDEMVAASGTILAGFYTPFGI
                     LSVCAVELFRHPDVVKACQNEPKLWERTVEELMRYKAHFNFFLPRVATEDVTLGEVDI
                     RAGQVVLPSIHAAATDPNSYPRPEVFDINRGPARCNIVFGAGPHFCPGAALSRQWLQV
                     GLERLFTTLPGLRLAKPYTDLEWQPGSISMPREVLVTWDRNRLPSVPS"
     gene            complement(1480..1626)
                     /locus_tag="VFH39_00300"
     CDS             complement(1480..1626)
                     /locus_tag="VFH39_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924256.1"
                     /translation="MRRRPAHATVPEPASQQNCHLVHVEHHKNLNAVKRATNKLTAFR
                     AVAI"
     gene            complement(1796..>2639)
                     /locus_tag="VFH39_00305"
     CDS             complement(1796..>2639)
                     /locus_tag="VFH39_00305"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET6924257.1"
                     /translation="PHELVRRIERFAPLDKVADPLAGLVGKVVAPRRVRNLLSGTRLG
                     HPAHPMLTDLPIGAWSMSTLLDTIGGNSGERAADLLVGAGILSAIPTAMTGLNDWSDT
                     IGGERRIGTVHAAANVAALGLYLASLIARRAGHRGRGKLLGLAGLGVLTAGGYLGGHL
                     SYAKAVNVNHTVFEYRPPEWTPVLADAELGEGEHRVVSADGASVLLVRDKGQLYALAN
                     TCSHAGGPLDEGEFVDGCVVCPWHASTFRLTDGGIVRGPASIPQPAYQTRVSEGKIEV
                     RSVE"
BASE COUNT          576 a          788 c          730 g          545 t
ORIGIN      
        1 gtccgcaatg acgggctatt cgccgtcact cacatcgaca gcgcgggcga actcgtcgaa
       61 acccaagtgg gcgacgacgc caccatcgac tggacccacg tcgtgcccgt cggcaacgac
      121 atcctgttcg tccgcaacga cgggctattc gccgtcgccc acatcgacag cgcgggcgaa
      181 ctcgtcgaaa cccaggtagg cgacgacgcc accatcgact ggacccacgt catcgcagtt
      241 gggtgaggtg agccccttca gctcggaaca gatggcaagc gattcctatc ccatgtaact
      301 agcacttcac gaggcataga gatcgaacct ggttgccact cgagatcggt atatggcttt
      361 gctaaacgta ggcccggcaa agttgtgaaa agccgttcaa ggccaacctg tagccactgg
      421 cgggataacg ctgcacccgg acagaaatgc gggcccgcac cgaacacgat gttgcatcga
      481 gcgggacctc tatttatatc gaatacctcc ggacgggggt acgaattcgg atcggtagct
      541 gctgcatgta ttgatggcag caccacttgc cccgcacgga tatctacctc tcccagtgtg
      601 acgtcttcgg tcgcaactcg tggtaaaaaa aagttgaaat gagctttgta acgcataagc
      661 tcttccacgg tccgttccca taatttaggc tcgttctggc aagcctttac aacgtcagga
      721 tgtcgaaaaa gttcgactgc gcatacactc aagattccaa atggagtgta aaacccagcg
      781 agtatggtac cggacgctgc caccatttca tcatgacata accgagtctt cttgaactca
      841 gcgaaaattt ttgaaagtag cctgtcgtca gggttatcga gtcttccttg aataagttcg
      901 ctgctgtact cgtataactc gttccaggca gcacaaatcc tagcccgatc gtcagcgctt
      961 tccacccgga ctaaaaccct gttagagaag ccatacaatt tatcgctttc atcttcagta
     1021 atacctgcag acagagcaac cgctctcacg ataaacggct caaaatagct cttattcagg
     1081 tcaggcgctc ggtgtacatc tcgcagcttg cttattaaat catccgcaac tccctcgact
     1141 tccttccgcc atggcgcaat agtttgctcg gagaaacgcg atatcatccc gcgccgaata
     1201 cgtctgatat ttggaacatc ctgccgcagc atgccactgc atgattgata tccagtacct
     1261 gtaactgtat cctcactcgg atccaatcta gccaagctaa atctcgggtc cgtcattatg
     1321 agacgcacta ttctcgcatc tctcgcaaca aagaccatct tacctgaagc aggcaacgtc
     1381 accggataca agtcgcctgg acgaaaacca aaataatgtt gcggttttcc acctattcgg
     1441 gattgaaacg ataatttctc cagtgtaggc gcgcccatat cagatggcca cggcgcggaa
     1501 tgctgtgagt ttgttggtgg cgcgcttgac ggcattgagg ttcttgtggt gctcaacgtg
     1561 gacgaggtgg cagttctgtt ggctggccgg ctcggggacc gtggcgtggg ccggacgtcg
     1621 gcgcaaggag ccaccacctc gcccccaggc ttaccagccg acaagagttt ccagtcgcat
     1681 aaaccactta gaattaatgt gaatgtctcg gcgcagccga atgactctgt ccatgatcta
     1741 gctccgggta atgagggacg gcgcggcagc gggtgagtcg gcgttgaccg gctgctcact
     1801 ccaccgagcg aacctcaatt ttcccctcgc tgacccgcgt ttggtaggca ggctgaggta
     1861 tcgaggctgg accgcgcaca atgccgccgt cggtgagccg gaaggtgctg gcgtgccacg
     1921 gacagacgac gcagccgtcg acgaactcgc cttcgtccag tggaccgcca gcgtggctac
     1981 acgtgttggc cagcgcgtag agctgtccct tgtcgcgaac cagcaggacc gaggctccat
     2041 cggcggagac cacgcgatgt tcgccttcgc cgagttccgc gtcggcgagc actggggtcc
     2101 actccggcgg gcgatactcg aagacggtgt ggttgacgtt gaccgccttg gcgtacgaca
     2161 ggtgaccacc aaggtatcca cctgccgtga gtaccccgag gccggcaagt cccagtaatt
     2221 tgccgcggcc gcgatgaccg gcgcgacgcg caatcagtga tgccaggtac agaccgagcg
     2281 cggccacgtt cgccgcggcg tgcaccgtcc cgatgcgtct ttccccgccg atggtgtccg
     2341 accagtcgtt gaggccggtc atggcggtgg ggatcgcgct gaggatgccg gccccgacaa
     2401 gtaggtcagc cgcacgttcc ccgctgtttc caccgatggt gtccagcaac gtagacatgc
     2461 tccaagcgcc gattggcagg tcagtcagca tggggtgggc tggatggccc aacctcgtcc
     2521 cgcttaacag gttacgtact cggcgaggcg ccacgacctt gccgaccaag ccggccagcg
     2581 ggtcagcgac cttgtccaag ggtgcgaaac gttcaatgcg gcgcaccagc tcgtgtgga
//
LOCUS       DASSFD010000014         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1007161, whole genome shotgun sequence.
ACCESSION   DASSFD010000014 DASSFD010000000
VERSION     DASSFD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007161"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..268)
                     /locus_tag="VFH39_00310"
     CDS             complement(119..268)
                     /locus_tag="VFH39_00310"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET6924258.1"
                     /translation="MSKYDKKSHEEVGKAMHEHKHDGKYKSKKQAVAAGLSKARREGS
                     SAPKK"
     gene            387..806
                     /locus_tag="VFH39_00315"
     CDS             387..806
                     /locus_tag="VFH39_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924259.1"
                     /translation="MKRLQHSHEAVPFSPRLLRVFAVAALLLVPWVLWVAIHAAPPHH
                     LGSRWNWAWGGLDVGELIGLAVTAYLGRHRSAWVIIAASATASLLLFDAWFDIWTARS
                     GFESWVSIWTASVIELPLSAALFWIAIRGVRFFTPKR"
     gene            871..1740
                     /locus_tag="VFH39_00320"
     CDS             871..1740
                     /locus_tag="VFH39_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HET6924260.1"
                     /translation="MSKKVLICGWFSFDEAIATIGDLEGCEVVEQWVRETGRQYDIAM
                     APYMQYGEDWRQVNPAEYDLLIFTTGPLKENELVNELLERFNHCTKWAVNVSLIHLSM
                     LDKFDRVWVRDSKRVIRPEMAFVGQTRLQPLVAVAYAPPQSEYPAGRHDLVRTMIHNW
                     LETRQLAAVELDMDMFAEHTYPRTPAQIESIIAKADVVVSMRLHATVLGLKYGKPVIA
                     CDPVSGGAKIYRQARHLRWPHVILPDDLSQTKLDQALQTCLSKPAAKQATASRQLALK
                     ELAALEKEFKDAL"
     gene            complement(1751..2650)
                     /locus_tag="VFH39_00325"
     CDS             complement(1751..2650)
                     /locus_tag="VFH39_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6924261.1"
                     /translation="MAALPFSSISIIYNPKSTGNSRRLAEKLQSQLAKELPDLTIDLR
                     ATEYAGHARELAREIADADTRPLIVSSSGDGGYNEVINGVMAAKNKDAICAVLAAGNA
                     NDHSRAIHKGRPLHEAILAGKIGRLDLLKVTIDEPGHELQELYAHSYAGLGLTPVVAT
                     ELNRQHHKLNALYEIYLSLRTFFRYRPFEIRHNGRVIELDSLLFANINQMAKILTLSP
                     RNRPDDGRFELVLLTAGRKFQLIRHLMQAAASSLDDAKRCKRYEFKTIKPLPMQLDGE
                     VCSLPAGSSVRVEALHHALRTIR"
     gene            complement(2757..>3061)
                     /locus_tag="VFH39_00330"
     CDS             complement(2757..>3061)
                     /locus_tag="VFH39_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6924262.1"
                     /translation="LRCHDNGSEKGVRMHVTRLPDDQALVLQQVAEDGEGDFASLVES
                     LRFDYGRLSHIVESLQHKGLVVLNRAGQSESWIRLSSKGRRLLAYMWPESGLSPSY"
BASE COUNT          615 a          872 c          869 g          705 t
ORIGIN      
        1 aaggcctcga ccatccgatc cagcgcttct tttgactccg catcggagaa gccggaatca
       61 tgctggattt ttttgataag ttcacggtat cccatatgca aatactcctt ccacatagtt
      121 atttcttggg ggcactactg ccttcccgcc tcgctttgct caaacccgcg gctacggctt
      181 gcttcttgct cttatacttg ccgtcatgct tgtgctcatg catggccttg ccgacttctt
      241 catgagattt cttatcgtac ttggacattt caatatcctc cgtttaccgt ggtaatggat
      301 agtactagag taacttgcct cacaataact cggctatgag ctatctaaca gggctgtcta
      361 cgctgccgtt tggtattatg tggcatatga agcgcttgca gcacagtcac gaagccgttc
      421 cattttcgcc gcgcctgctc cgggtctttg ccgtggctgc cttactgctg gtgccgtggg
      481 tgctgtgggt ggcgatacat gccgctccgc cccatcattt agggtcgcga tggaattggg
      541 cctggggcgg tctggatgtc ggggaactca tcggtctggc ggttaccgcg tacctcggac
      601 ggcaccggtc cgcctgggtg attattgccg cctcggcgac tgcgagcctt ttgttgttcg
      661 atgcctggtt cgatatatgg accgcccgca gcggctttga gtcctgggta tcgatctgga
      721 cagccagcgt gattgagttg ccgctgagcg cggccctgtt ctggattgca atccgcggcg
      781 tcagattctt tacgccgaag cgctgagctt accgctcggt tataggaatt cttactggca
      841 ccctcgcagc gccatgccac aatggcagcg atgagcaaga aggttctaat atgtgggtgg
      901 ttcagcttcg acgaggctat cgccaccatc ggtgacctgg aaggttgcga ggtagtcgaa
      961 cagtgggtac gtgaaaccgg gcggcaatac gacatcgcca tggcgccgta tatgcagtac
     1021 ggcgaagatt ggcggcaggt caatccggca gagtacgatc tgttgatatt taccaccggt
     1081 ccgttaaagg agaatgagct cgtgaacgag ctgttggagc ggttcaacca ctgtaccaag
     1141 tgggcagtga acgtctcact tatacattta agtatgctcg ataaattcga ccgcgtgtgg
     1201 gtccgtgaca gcaaacgggt catacgaccc gaaatggcgt tcgtgggcca gacccggttg
     1261 cagccgctgg tcgcggtagc ctatgcgccg ccgcagtccg agtacccggc tggccggcat
     1321 gacctggtcc ggacgatgat tcataactgg ctggaaaccc ggcagctggc ggccgtggag
     1381 ctcgacatgg acatgttcgc cgagcatacc tatccgcgga caccggcgca gatcgaatcg
     1441 atcatcgcta aagccgacgt ggtggtaagc atgcgtctgc acgccactgt cctggggctg
     1501 aagtacggca aaccggtaat cgcctgcgac cccgtgagcg gcggcgccaa aatctaccgt
     1561 caagccaggc acctgcgctg gccacacgtt atcctgcccg acgacctaag ccagactaaa
     1621 ctcgaccaag cgctacaaac ctgcctgagt aaaccggccg ccaagcaagc caccgccagt
     1681 cgtcaactgg ccctgaagga gctggcagca ctcgaaaagg aattcaaaga cgcattgtaa
     1741 cgccggttgc ctagcggatt gtgcgtaacg cgtgatgcag cgcttcgacc ctgacgctgc
     1801 tgccggctgg cagtgagcag acctccccgt ccagttgcat cggcagcggc ttaatggtct
     1861 tgaactcgta acgcttgcaa cgcttggcgt catccagact gctggccgcc gcctgcatca
     1921 agtgccggat cagttggaat ttccggccgg cagtcagcaa gacaagttcg aaacggccgt
     1981 cgtcgggtcg attgcggggg gataaggtaa ggatcttggc catctggtta atattggcaa
     2041 acagcagact gtccagttcg ataacccgtc cgttatgccg gatctcgaag ggccggtagc
     2101 ggaaaaaggt ccggagactc aaataaatct cgtagagagc attcagttta tgatgctggc
     2161 ggttcaactc ggtcgcaacg accggggtaa ggccgagtcc ggcgtaggag tgagcgtaaa
     2221 gctcctgcag ttcgtgaccg ggttcatcga tggtcacctt cagcagatcc aggcggccga
     2281 tcttgcctgc cagtatcgcc tcgtgcagcg gccggccttt gtggatggcc cggctatggt
     2341 cgttggcgtt acctgctgcc agaacggcgc atatcgcgtc tttattcttc gctgccatga
     2401 caccgttgat cacctcgttg taaccgccgt caccgctgga cgacacgatg agtggccgtg
     2461 tatcggcgtc ggcgatttcg cgcgccagct cgcgggcatg gccggcgtat tcggtcgcac
     2521 gtaaatcgat agtcaggtcg ggcaattcct tcgccagttg actttgtagt ttttcggcca
     2581 ggcggcggct gttcccggtg ctcttcgggt tgtaaatgat actgatgcta ctgaaaggta
     2641 atgccgccat tgccctctaa gcgtaccacc ctctgccgct tttaactgtg taatccgaaa
     2701 tgaaaagtcc gccgtttgcg aggaggacgg cggacttcct tagtatccgt gacgtactaa
     2761 tagctcgggc tgaggccgct ttccggccac atgtaggcta gcaaacggcg acccttggaa
     2821 ctcaaccgga tccagctttc gctttgcccg gcacggttca gcacgacgag acctttatgt
     2881 tgcaacgact cgacgatatg ggacaaccga ccgtagtcaa agcgcaggct ctccaccaga
     2941 cttgcaaaat caccttctcc atcttccgcc acctgctgca ggaccaacgc ctggtcatcc
     3001 ggcaaacgtg ttacatgcat acgcaccccc ttttcgctgc cgttatcgtg gcaacgtaac
     3061 a
//
LOCUS       DASSFD010000015         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_875255, whole genome shotgun sequence.
ACCESSION   DASSFD010000015 DASSFD010000000
VERSION     DASSFD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875255"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VFH39_00335"
     CDS             <1..1578
                     /locus_tag="VFH39_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET6924263.1"
                     /translation="AVYTAPYQTAKGVIYMSAKVQSIITVGSTGIVADIECHLSNSLP
                     GIVIVGFANKAVDEAKERLRGAFANSNLQLPRKRITLNLAPADVPKDGSAFDLACAAA
                     IMAAGNQIKHKFTEAEALLGELGLDGSVRPVRGIIGKLLAGRAKGITHFYIPEANLRQ
                     AQLVPNITLTPVSELAGLYQHLCNTLNISPVQTNQGIYEPEQANRSDDEPVTLGDIIG
                     QAQAKRALMIAAAGGHNVFFSGPPGTGKSMLAKALPGLMPALTQEEMLEVTHLHSLAG
                     HEYDRIITARPFRAPHHSATHVAVIGGGQNLRPGEISLSHRGILFFDELPEFNRQTLE
                     ALRQPLEDRVISVARAKDTAEYPASFVLVATANPCPCGYYGTAKTCRCLPHQIQRYQN
                     RISGPILDRIDLYTEVNEVEHSKLLNRAADDSEQLAAIDRVLRARKAQAKRFKGTHKL
                     NKDMTNADLKRLAQLSAAAKDLLDVAAQRLELSARAYMRSLKVARTIADLDGSATIEP
                     EHISEALQYRSQQKAAL"
     gene            1688..2266
                     /gene="infC"
                     /locus_tag="VFH39_00340"
     CDS             1688..2266
                     /gene="infC"
                     /locus_tag="VFH39_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET6924264.1"
                     /translation="MNEQIRAPQLRVIDEDGKQLGILSRNEALEIARERELDLVEISP
                     DAKPPVAKVVDWGKYNYQKTKQQNKNKKAAKALEMKQMRFGLKIGDHDLDIKMGKVRK
                     FLEAGHKVKITIFYRGRELAHKEIGFKLAERVINDIFGDSIIVDQNPQFAGKQLNFVI
                     RHNPRAKVATPVGAGAEAEEDKENNDAKTEDA"
     gene            2244..2438
                     /gene="rpmI"
                     /locus_tag="VFH39_00345"
     CDS             2244..2438
                     /gene="rpmI"
                     /locus_tag="VFH39_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET6924265.1"
                     /translation="MPKLKTHSGTKDRVKLTKNGKVRVGSPNRNHFLQKKSGSRKRAL
                     RGLSTLAGKNARNLKLKLGA"
     gene            2607..2948
                     /gene="rplT"
                     /locus_tag="VFH39_00350"
     CDS             2607..2948
                     /gene="rplT"
                     /locus_tag="VFH39_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET6924266.1"
                     /translation="MRVKRGVAAHKKHVKVRHGTKGMTHGNRASVKRGRQAITKGLQF
                     ATRDRRNRKRTFRELWNTRINAAARQNGTTYSKLIAGLKANNVTIDRKVLAELAVNEP
                     KAFEAVVKAAK"
BASE COUNT          757 a          960 c          826 g          459 t
ORIGIN      
        1 gcggtgtaca ctgcgccata ccaaacggca aagggagtca tatatatgag tgccaaggtc
       61 cagagcatca tcaccgtcgg cagcaccggc atcgtggcag acatcgagtg ccacctcagt
      121 aattccctgc ccggcatcgt catagtaggc tttgccaaca aagcggtcga cgaggccaaa
      181 gaacggctca ggggtgcctt cgccaacagc aacctgcaac tgccccgcaa gcggatcact
      241 ctcaacctgg cgccggccga tgtaccgaag gacggcagcg ccttcgatct ggcctgcgcc
      301 gccgccatca tggccgccgg taaccagata aaacacaaat ttacagaggc ggaagccctg
      361 ctcggcgagc ttggcctgga cggctcggtg cgcccggtgc gaggtatcat cggtaaatta
      421 ctcgccgggc gggccaaagg cattacgcat ttctacattc cggaggccaa tctgcggcaa
      481 gctcagctcg tacccaacat tacgctcacc cctgttagcg aactagccgg attataccag
      541 catctctgta acacattgaa catctcccct gttcaaacca accagggcat atatgagccc
      601 gaacaagcaa accggagcga cgacgagccg gtgaccttgg gcgacataat cggccaagcc
      661 caggccaagc gggcgctgat gatcgccgca gccggcggcc acaacgtgtt cttttccggc
      721 ccgcccggca ccggcaaaag catgctggcc aaagccctgc ccggtctgat gccggcgctc
      781 acccaagagg aaatgctgga ggtcacccac ctgcacagcc tggccgggca cgagtacgac
      841 cgcatcatca ccgctcggcc gttccgggcg ccgcatcaca gtgccacgca cgtggcggtc
      901 atcggcggcg gccagaatct gcgtccgggc gaaatcagcc tcagccaccg gggcatcctg
      961 ttcttcgacg agctgccgga gttcaaccgc cagacgctcg aagcgctgcg ccagccgctg
     1021 gaagaccgtg tcatcagcgt cgcccgcgcc aaagacaccg ccgaataccc tgctagcttc
     1081 gtgctggtgg ccacggccaa cccttgtccc tgcggctact acggcacggc caagacctgc
     1141 cgctgtctgc cgcaccagat ccagcgctac cagaaccgta tttccgggcc gattcttgat
     1201 cggatagacc tatacaccga agtgaacgaa gtcgaacaca gcaagctgct gaaccgggca
     1261 gctgacgaca gcgagcagct ggcggccatc gaccgcgtgc tgcgggcccg taaagcccaa
     1321 gccaaacggt tcaagggcac gcacaagctc aacaaagaca tgaccaacgc cgatctgaag
     1381 cgtctggccc agctcagcgc ggccgccaag gacctgctgg acgtcgccgc gcagcgcctg
     1441 gagctcagcg cccgagccta catgcgttca ctcaaggtcg cccgcaccat tgccgacctc
     1501 gacggctccg ccaccatcga acccgagcac atcagcgaag ccctgcaata ccgcagtcag
     1561 cagaaagcgg cgctgtgaac atagaccacc gaatgtggca cgtttgacct ctgaagctat
     1621 ctctgttaca atacttacac tttaaaataa caagtaagga cggataagta tcagcaaatt
     1681 cacgcgcctg aatgaacaga ttcgggcacc gcagctcagg gttatcgacg aggacggcaa
     1741 gcaacttggc atcctcagcc ggaacgaggc gctggaaatc gcccgtgaac gggagttgga
     1801 cctggtggag atttcaccgg acgccaagcc gccggtagcg aaagtggtcg actggggtaa
     1861 atacaactac cagaagacca agcagcagaa caagaacaag aaagctgcca aagccctcga
     1921 gatgaagcag atgcgcttcg ggctgaagat cggtgaccac gacctggaca tcaagatggg
     1981 caaggtgcgg aagttcctgg aagccgggca caaggtaaag atcaccatct tctaccgcgg
     2041 ccgcgagctg gcccacaagg aaatcggctt caaactggcc gagcgggtca tcaacgacat
     2101 cttcggtgat agcatcatcg ttgaccagaa cccgcaattc gctggtaaac aactgaactt
     2161 cgtcatcagg cacaatccgc gtgccaaagt cgccaccccg gtgggtgccg gcgccgaagc
     2221 ggaagaagat aaggagaaca acgatgccaa aactgaagac gcatagcggt accaaagacc
     2281 gcgtcaagct taccaagaac ggcaaggtgc gcgtaggctc accgaaccgc aaccacttct
     2341 tgcagaagaa gtcgggtagc cgcaagcgcg cgctgcgcgg cctcagcacc ctggccggca
     2401 agaacgcccg caacctgaag ctgaaattag gggcctaagg cgccaggcta ccgaagctgg
     2461 aagctggatt ctggatttta gaacaagcgc tccaggcgaa ctattctaaa atctataatc
     2521 cagcttccag aatcaaacaa gaggcgcgtc cggtatatca tccaccggcc agcccataac
     2581 caaccctaac agtaaggagt taagcgatga gagtcaaacg aggcgttgcc gcccacaaga
     2641 agcacgtaaa agtccgccac ggcaccaagg gcatgaccca tggtaaccgc gccagcgtaa
     2701 agcgcggccg ccaggccatt accaaggggc tgcagttcgc cacccgcgac cgccgcaacc
     2761 gcaagcgcac cttccgtgag ctgtggaaca cccgcatcaa tgccgctgcc cgccagaacg
     2821 gcaccaccta cagcaagctg atcgccggcc tcaaggccaa caacgtcacc atcgaccgca
     2881 aggtcctggc cgagctggcc gtgaacgagc cgaaggcttt cgaagccgtc gtcaaagccg
     2941 ctaaatagtt ctagattggc ttggacaaga aaaacacccc gggtcaccgg ggtgtttttc
     3001 at
//
LOCUS       DASSFD010000016         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_177122, whole genome shotgun sequence.
ACCESSION   DASSFD010000016 DASSFD010000000
VERSION     DASSFD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177122"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..78)
                     /locus_tag="VFH39_00355"
     tRNA            complement(2..78)
                     /locus_tag="VFH39_00355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Arg,seq:tcg)
     gene            256..1278
                     /locus_tag="VFH39_00360"
     CDS             256..1278
                     /locus_tag="VFH39_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6924267.1"
                     /translation="MLGNETAQQTITQSSDTFKHITDTLFGGDTGRSLLVLLIALFVG
                     SVIGKLLSMVLRRMSRHVGRRADASSDLNTVNRLRRIETWTILSIAIVRVVCLVLALY
                     FWWVFIHPHGRPTALVGASAVLIVILGGVFSPLLRDFAFGGGMMAEHWFGVGDLITIQ
                     PFDIKGVVERITLRSTRIRGLNGEVIWVANQNISSVRIAQKGVWTMALELFVTDAEKA
                     EELLEKTNALLPDGPSLLANPLAIMTAKERADNLWHLTAVGEVAPGREWLIEKTAIDL
                     MKKLDEQSRKPILISDPVARFADNDTERQFARAVRNAKKTRRTSRRKLPLTAKRKQEA
                     SPNRNR"
     gene            1294..>2243
                     /locus_tag="VFH39_00365"
     CDS             1294..>2243
                     /locus_tag="VFH39_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6924268.1"
                     /translation="MTEVTTFAEAHAALRKYYQVPLNGPYTLDRMYRLLAAVGNPQDT
                     LRAIHIAGTSGKTSTAYYAAALLQQTGAKVGLTVSPHVDEVNERVQLNLTPLPEAQFC
                     AELTEFLALVEASDITPSYFECMIAFAYWEFARQQVDYAVVEVGLGGLLDGTNTIQRD
                     DKVCVIADIGFDHTEVLGDTLEAIAAQKAGIIQPHNAVFVHPQDEAVMAVFQEACHAK
                     VAELTVVPDTLPFDSLYLPSFQRRNLSLALAAVKYVLQRDGRNPLDDGQIETAARTHI
                     PARMETVKSGGRTFILDGAHNAQKLATLFDDVERRFPGQE"
BASE COUNT          496 a          617 c          668 g          462 t
ORIGIN      
        1 atggtacacc cggcaggatt cgaacctgcg accccttggt tcgaagccaa gtactctgat
       61 ccactgagct acgggtgcgc gctgaacaag ctgcatttta acagatataa cagagactga
      121 aaagcagctt tgaaatcaaa agtttaaatt gacaagggta gcagacttcg ttatcataag
      181 agtaatgagt ccggactaaa gcggcgttcg gacgcgacaa tcaaacaaat aaaccttttg
      241 aaaggaggtg cggctatgct cggcaacgaa acggcgcagc agacaatcac ccagtctagc
      301 gacaccttca agcacatcac cgacacgtta ttcggtgggg ataccggccg gtcgttgttg
      361 gtgttgctga tagccctgtt tgtcggctcg gtcatcggca agctgctgtc aatggtgctg
      421 cggcggatga gccgccatgt cggccggcgt gccgatgcct caagtgattt gaacaccgtc
      481 aaccggctgc gccggattga aacctggacc atcctgtcta tcgccatcgt ccgcgtcgtg
      541 tgcctcgtgt tggcgctgta tttctggtgg gtattcatcc atccgcatgg gcggccgacg
      601 gcattggttg gcgccagtgc ggtattgatc gttatcctcg gtggtgtttt tagccccttg
      661 ctgcgcgatt tcgccttcgg tggcggtatg atggccgagc actggttcgg agtcggcgac
      721 cttataacca tccaaccgtt cgacatcaag ggcgtagtgg agcggataac gctgcgcagc
      781 acgcgcatcc gggggctgaa cggggaagtg atatgggtgg cgaaccagaa catatcgagc
      841 gtgcgcattg cacaaaaggg tgtctggacg atggcgcttg agctgttcgt gaccgacgcg
      901 gaaaaagccg aggagctgct cgagaaaacc aatgctttgt tgcccgacgg cccttcgttg
      961 ctggctaatc cgctggcgat catgaccgcc aaggagcggg ccgataatct gtggcatctg
     1021 accgctgtcg gcgaagtggc gccgggtagg gaatggctga tcgagaaaac ggccatagac
     1081 cttatgaaga aactagacga gcagtcgcgc aagccgatcc ttatcagcga cccggttgct
     1141 cgcttcgccg acaacgacac cgagcgccag ttcgcccggg cggtgcgcaa cgccaaaaag
     1201 acccggcgca ccagccggcg taaattgccg ctgacagcca agcgcaaaca agaagcttcg
     1261 ccaaaccgta accggtaggg tactatagtg gtcatgacag aggtgaccac cttcgctgaa
     1321 gcccacgcgg cactaaggaa gtactaccag gtgccgctga acgggccgta caccctagat
     1381 cgcatgtatc ggctgctggc agccgtcggc aatccacaag ataccttgcg ggcgatacat
     1441 atcgccggta cgtcaggcaa gacgtcgacg gcatattacg cggctgcttt gctgcagcag
     1501 actggtgcga aagtcgggct gacggtttcg ccgcatgtcg acgaggtcaa cgagcgtgtg
     1561 caactgaacc ttacgccgtt gcctgaggcg caattctgtg ccgaactaac cgagttcctg
     1621 gcgcttgtcg aggcatctga tatcaccccg tcttacttcg agtgcatgat tgcattcgcc
     1681 tattgggaat tcgcccgcca gcaggtcgat tacgcagtgg tagaggttgg gctgggtggt
     1741 ctgctcgacg gcacgaacac cattcagcgc gacgacaagg tgtgcgtcat cgcggatatc
     1801 ggttttgacc ataccgaagt gcttggcgac acgctggaag caatcgccgc ccagaaagcc
     1861 ggcattatcc agccgcataa cgctgtcttc gtacacccgc aggacgaggc ggtcatggct
     1921 gtgttccaag aagcgtgcca tgccaaggtc gcggagctca ccgtagtccc agatacatta
     1981 ccgttcgata gcctctattt gccctctttc caacgccgga acctgagcct ggcgctggca
     2041 gctgtaaagt atgtgctgca gcgtgacggg cggaatccgc tcgatgacgg acagatcgaa
     2101 actgctgccc ggacgcacat tcccgctcgt atggagacgg tgaaatccgg cggccgtacg
     2161 tttatattgg atggtgcgca caatgcccaa aagctcgcca cgttgttcga cgatgtggag
     2221 cggcgctttc cgggtcagga aat
//
LOCUS       DASSFD010000017         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_521153, whole genome shotgun sequence.
ACCESSION   DASSFD010000017 DASSFD010000000
VERSION     DASSFD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521153"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..588
                     /locus_tag="VFH39_00370"
     CDS             61..588
                     /locus_tag="VFH39_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924269.1"
                     /translation="MKRMCQAAVAALAALVVAVPATAAHAAPAPTVSRHTTTSAVAWP
                     TADGRVANAKHVAVITTHKAHRHHGTLVWYRDPKTHKVQQVVGIYSPAGVTNCYRCGH
                     IAVRHGVARLVLPKGVRFERGSRIYQYDHGKQMRLHWLGQPRSVHDTVNGDPAVLYVA
                     TAELSVPDRYAFPKG"
     gene            complement(681..1094)
                     /locus_tag="VFH39_00375"
     CDS             complement(681..1094)
                     /locus_tag="VFH39_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924270.1"
                     /translation="MSSTITATDLGLDLRKADEQTMFKWFLASYLFGKRIQQDIAKRT
                     WEVFMHAGIDTPRKIHNRSWQELVDLLGEGHYKRYDESTARNLLDMSQQLLDDYKGKL
                     TTMRAAATSKADFGKKLQQLKGVGPKTAEIFLREL"
     gene            1336..1791
                     /locus_tag="VFH39_00380"
     CDS             1336..1791
                     /locus_tag="VFH39_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924271.1"
                     /translation="MQAKTTNSEQVWQSPDGKRTIWEVQLQGDDGNEYRLKTYSSEIA
                     KLGFKGKVKSYLNKFGERFVRQDVETTETGGGYSRDDNAIRAQWAIGQAINLASVKMD
                     KQEITLPVIEEYAKQLFTTVSRVKGEELSEQEAAAGEAHIKNFTQAAAF"
     gene            1907..>2344
                     /locus_tag="VFH39_00385"
     CDS             1907..>2344
                     /locus_tag="VFH39_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924272.1"
                     /translation="MIIAYILAALAAVANAASSLLRREGNRRQRTIAPYRRPVWWAGV
                     GAATVSFGLQAGALGKGSLAAIEPILALELVLVVFGAGLFFHMHIWVREWVSTGLLTV
                     GTIVLVVALDPQKSHQISVLVWQWVVALGTSVAVIVASFLIATR"
BASE COUNT          504 a          664 c          717 g          459 t
ORIGIN      
        1 gacgcaaacg gcgtcatatc cgagcagctt tcaggctgcc aacatcgaga gggtaggact
       61 atgaagcgaa tgtgtcaggc agcagtcgct gcgctggcgg cccttgtggt cgctgtgccg
      121 gcaactgctg ctcacgcagc acccgcaccg acggtatcgc gccacacgac gaccagcgcg
      181 gtcgcctggc cgacggccga cggccgcgtc gcaaacgcca agcacgtggc agtcatcacg
      241 acgcacaagg cgcaccgcca tcacggaacg ctcgtgtggt accgcgaccc caagactcac
      301 aaggtccagc aggtcgtcgg catctacagc cccgcgggcg tcacgaactg ctaccgctgc
      361 ggccacatcg ccgtccggca tggtgtcgcc aggctggttc tcccgaaggg ggtccgcttc
      421 gagcgcggca gtcgcatcta ccaatacgac cacggcaagc agatgcgtct gcactggctc
      481 ggccagccgc gcagcgtgca cgacacggtc aacggggacc ctgcggtcct gtacgtggcg
      541 accgcggaac tgagcgtacc ggatcggtac gccttcccca aggggtagtt gcaaggggcc
      601 ggactccgaa acagccggag tccggcccta acccccctgc atcactgtcc tacccctcga
      661 acttacttcc tgtttatcgc ttataactcg cgcagaaaaa tctcggccgt cttgggtccg
      721 acacctttca gctgctgcag ctttttgccg aagtcggctt tgctggtagc ggcagcacgc
      781 atggtggtca gtttgccttt gtagtcatcc agcagctgct gcgacatgtc gagcaggttg
      841 cgggcggtgg attcgtcgta gcgcttgtag tggccctctc cgagcaggtc gaccagttcc
      901 tgccaggagc ggttatgaat tttgcggggc gtgtcgatgc cggcgtgcat aaagacttcc
      961 cacgtgcgct tggcgatgtc ctgctggatt cgtttgccga aaaggtaact ggccaggaac
     1021 catttgaaca tagtctgctc gtccgccttg cgcaggtcga ggcccagatc ggtggccgtg
     1081 atggtgctac tcatgccgag atagtagcat gtggtctggc ggccgttctg ttaagaaaat
     1141 tacgctgtcc gaatgtgcgc ggaatcatac aaaataagcg ctagcagtct tgactaatga
     1201 gcgccaaatt tgctatactt aatatagtaa ataacagagg tagaggaggg cgaagcagca
     1261 cagtagacga agcatttttt acgagacaaa agcaatagcc aattacgttc aagacgaagc
     1321 aataaggaga atcacatgca agctaaaact accaatagtg aacaggtctg gcagtcgccg
     1381 gacggtaagc ggaccatttg ggaggtccaa ctgcagggcg atgatggtaa tgagtaccgg
     1441 ctgaagacct acagctcgga aatcgccaaa ctcggtttta agggcaaagt aaagagctac
     1501 ctgaacaagt tcggcgaacg gttcgtccgc caggacgtcg aaacgaccga aacaggcggc
     1561 ggttactcgc gcgatgacaa cgccatccgc gcgcagtggg cgatcgggca ggccatcaac
     1621 ctggccagcg tgaagatgga caagcaggaa ataaccctgc cggtcatcga ggaatacgcc
     1681 aagcaactgt tcaccacggt cagccgggtg aagggcgaag agctcagcga gcaggaagct
     1741 gccgccggtg aagcccacat caaaaacttt acccaagccg cagccttcta gattgccgaa
     1801 actacaggaa accgtccggg agaccggacg gtttttatgt atatacgctg gacagtaaga
     1861 atcttcgctc cggctgcgct tgccggctgc tatgctgaaa tcgcctatga ttatcgctta
     1921 catattggcc gccctggccg ccgtagccaa tgccgcttcc agtcttctgc ggcgggaagg
     1981 gaaccgccgg cagaggacca tagcgcctta tcggcggcct gtctggtggg cgggtgtggg
     2041 cgcggccacg gtgtcgttcg gcttgcaggc cggagcgctc ggtaaaggca gtctggcggc
     2101 gatcgaaccg attctggcac tcgagctcgt gctggtggtc tttggagcag gcctgttctt
     2161 ccatatgcat atttgggtgc gtgagtgggt cagtacgggg ctgctgacgg tgggtacgat
     2221 cgtgctggtg gtggcgcttg acccgcaaaa aagccatcag atatccgtac tggtgtggca
     2281 gtgggtcgtg gcactcggta ccagcgtggc ggtcatcgtc gccagctttc ttatcgccac
     2341 gcgt
//
LOCUS       DASSFD010000018         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1370079, whole genome shotgun sequence.
ACCESSION   DASSFD010000018 DASSFD010000000
VERSION     DASSFD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370079"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VFH39_00390"
     CDS             complement(<1..1407)
                     /locus_tag="VFH39_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924273.1"
                     /translation="MNSLFGRSSPLAVLKKLLGTGLLVLLVGIAVNGSAQATVSVDQQ
                     PLTIQKPLPPNIVLMLDDSGSMAWDFMPDAGYLSGKFTAYDENGYRDTFVGADALRSN
                     SVNGLYYNPGTTYKPPVYANGTSYPTPSGLASAYANPFLSTASTDTVDVTTYRSPWLC
                     DYTGYDTTQSGGNYPCPNSLYNAFPYFTAFPSGGASQPSLSCPYGGAVSSNGFCNSSQ
                     YGYYVATVSCSGGKTYRAATGWCESSSTSYIYLFTYTKPGTTAGSYVRYYVGKSATDC
                     AAAPSSSTCVYDTATQKNVATWFSYYRRRILMAKSGVMSAFSGIDATFRVGFGSIDGG
                     DSGNKNYLNLPTSQYQYADAYNGGNNYIAQVQAFGDGSAGTQKSSLWTWIAAAKASGG
                     TPLRQALDAAGTYYGTDQPWQSKNASTNATEELACRQSYTILTTDGFWNGKDLGNGNI
                     DNTAATLTGANQQTYTYTP"
     gene            complement(1469..2119)
                     /locus_tag="VFH39_00395"
     CDS             complement(1469..2119)
                     /locus_tag="VFH39_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HET6924274.1"
                     /translation="MRECVLSQQAVASLATPSRRPSSQAAIGRDMRDARLPARQAQQG
                     IALVVALVLLLVITLVGLAAVRGTIMQQKMSANLYDREIAFQSAEAALRTAAALVPSH
                     PALIARNCRTGGTVCLTNPFSDTLPAGSIHDLSTTDYAASTLAAGQPQYVIENMGNWA
                     DPSTDTGYGQTANSHNYGAQGLSSTAVYYRITARSSDPAVTGDRAVVVLQAVIKQG"
     gene            complement(2121..>2325)
                     /locus_tag="VFH39_00400"
     CDS             complement(2121..>2325)
                     /locus_tag="VFH39_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924275.1"
                     /translation="GVTKMSLGYHQASAAGFVAANLVTDWSKVDAVQMSLTVQSDDQT
                     AGVAAKPLERTFTATTAVRNRVK"
BASE COUNT          463 a          651 c          730 g          481 t
ORIGIN      
        1 cggagtgtag gtataggtct gctggttggc cccggtcagc gtggctgcgg tattgtcgat
       61 gttgccgttg cccagatcct tgccgttcca gaaaccgtcg gtggtgagga tggtgtacga
      121 ctgccggcag gccagctcct cagtagcatt ggtcgatgca ttcttggatt gccaaggctg
      181 gtcagtcccg taataggtgc ccgcggcatc caaggcttgg cgcagcggcg tgccgccaga
      241 agctttcgca gcagctatcc atgtccacaa gctgcttttt tgggtgccag cggagccgtc
      301 accaaacgct tgcacttggg caatataatt gttgcctccg ttgtaagcgt cagcgtattg
      361 gtattgggag gtcggaagat tcaggtaatt cttgttaccg ctgtcgccgc catcgatcga
      421 accaaagccc actcgaaacg tagcatcgat accagaaaag gcgctcatta caccactttt
      481 ggccatcaaa atgcgccggc gataatatga aaaccaagtc gcgacgtttt tctgcgttgc
      541 cgtatcatac acgcaggtac tactcgaagg tgccgcggca caatcggtgg ccgatttgcc
      601 gacgtaatac ctcacgtagc ttcccgcagt ggtaccgggc ttggtatatg taaaaagata
      661 aatgtagctg gtacttgagc tttcgcacca accagtggcc gcgcgatagg tcttcccacc
      721 ggagcaactt actgttgcaa cataataccc atactgcgaa ctattgcaaa aaccattact
      781 gctgactgca ccgccatatg ggcaactgag cgacggctgc gaggcaccac cggaaggaaa
      841 ggcagtaaag tacggaaacg cgttgtaaag cgagttgggg caggggtagt taccgccgga
      901 ttgcgtcgtg tcatagccgg tgtagtcgca caaccatggc gaacgatagg tagtgacatc
      961 caccgtgtcc gtgctggccg tactcaggaa cgggttggcg tacgcactgg ccaagccaga
     1021 cggcgtcggg tacgaagttc cattcgcata aacaggcggc ttataggttg taccaggatt
     1081 gtaataaagc ccgttgacgc tgttactgcg caatgcatca gcgccaacga aagtgtccct
     1141 ataaccgttc tcgtcgtacg ccgtaaattt gccgctcaga tatccggcgt ccggcatgaa
     1201 atcccacgcc attgaaccag aatcgtccag catcagaacg atattcggcg gcagcggctt
     1261 ctggatcgtc agcggctgct ggtcgaccga aacggtcgct tgtgcagagc cattaacggc
     1321 gatgccgacc agcagtacca gcaggccggt gccaagcagc ttcttaagca ccgcgagggg
     1381 gctgcttcgt ccgaagaggc tgttcatagg tgggttctcg agatgttgaa agcaccggtt
     1441 gcctgatagc cgccggtggt taaaggggtc agccctgctt gatgaccgcc tgcagcacga
     1501 ccacggcgcg gtcgccggtg acggccggat cactgctccg ggcggtgatg cggtagtaca
     1561 cggcggtgga ggaaagcccc tgcgcgccat agttgtggga gttcgcggtc tggccgtaac
     1621 cggtatcggt actggggtca gcccagttgc ccatgttttc gatcacgtat tgcggctgcc
     1681 ctgcagccag ggtgctggcg gcgtagtcgg tcgtggacag gtcgtggatg ctgcccgcgg
     1741 gaagcgtgtc cgagaaggga ttagtcaggc acacggtgcc gcccgtgcgg caattgcggg
     1801 cgatcagtgc cgggtggctg ggtaccagcg cggctgccgt gcgcaaggcg gcttcggcac
     1861 tctggaacgc gatctcgcgg tcatacaagt tggcggacat cttctgctgc atgatcgtgc
     1921 cgcgcacggc ggccaggccg accagggtga tgaccagcag caggaccagt gccacgacca
     1981 gcgcaatgcc ttgctgggcc tgtctggcag gcaggcgcgc atcacgcatg tcgcggccga
     2041 tggctgcctg gctggagggc cgtctggacg gggtcgccaa gctggcgacg gcctgttgag
     2101 aaagcacaca ttcgcgcatg tcatttcacc cgattgcgga ctgcggtggt cgcggtaaag
     2161 gtgcgctcga gcggcttggc cgcgacgcct gcggtctggt cgtcactctg cacggtcagg
     2221 ctcatctgca ctgcgtcgac cttgctccag tccgtgacca ggttggcagc cacgaacccg
     2281 gcggcactgg cctggtggta gcccagactc atcttggtaa cgcca
//
LOCUS       DASSFD010000019         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1576043, whole genome shotgun sequence.
ACCESSION   DASSFD010000019 DASSFD010000000
VERSION     DASSFD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576043"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..792)
                     /locus_tag="VFH39_00405"
     CDS             complement(139..792)
                     /locus_tag="VFH39_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6924276.1"
                     /translation="MFEIAIVAGFGLLTYHHARVRDDEFAGVSRKVGDRMGKLWDIAH
                     QGMRENRFLRAEKALLTILKIDEKNAAAYNRLGILYAKQKEFRDAIDCFEIASSMEAT
                     PSSLHNLGLIYYETENYEKAAIAFEQALKLEDNLAARHVAYAKVEEKLGNDKLMFQEL
                     ERAAELEPNKETYSLLYKAFESRGMMAEADLIADKMNKLIVPAGRPKRIVRPRRVVV"
     gene            complement(819..1664)
                     /locus_tag="VFH39_00410"
     CDS             complement(819..1664)
                     /locus_tag="VFH39_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924277.1"
                     /translation="MTGFILHPKTTEAVERLLASRPHAAVLEGPAGSGKRELARYLAA
                     QITGKPDISLALREVYIVTPEGSKAVSVEQIRSIEQFLSRKLPGNGARVVIIESAHGM
                     GREAQNALLKTLEEPPADASIILLADSAASLLPTVRSRAVTVRLLTPDKDELRRYYVA
                     AGHDATAVERAILMSGGLPGLVEAILTGDEESELTAAAEQARAIIQGTRFQRLALVDG
                     LSKQRDLALHVCQLLQQMAHVRLAAGAITPQWQRIMQAAYTAEERLHTSGQPKLVLTD
                     LMLNL"
     gene            complement(1661..2554)
                     /locus_tag="VFH39_00415"
     CDS             complement(1661..2554)
                     /locus_tag="VFH39_00415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6924278.1"
                     /translation="MSNLEISRAQRLDQKVVELLPELSRAYAAKLVEQGKVTVNGEVE
                     TKAGYKLREGDAVEVDYRPEEAQAIPDIELPILYEDDDCVVINKPIGVLTHSKGAFNP
                     EATVATWLRRRLAKGEWQGTTDAAGNARAGIVHRLDRATSGVMICAKTPEALSWLQKQ
                     FSQRRTKKTYAAVVSGHLPEDHAVIDMPIERNPKKPQTFRVGVNGKPSVTEYQVVSSN
                     SAASLLELRPQTGRTHQLRVHLSHLGHPIVGDTLYGGLAADRLYLHAERLELTLPSRE
                     RKVFEAPVPKVFTDYLAEADS"
     gene            complement(2551..>3150)
                     /locus_tag="VFH39_00420"
     CDS             complement(2551..>3150)
                     /locus_tag="VFH39_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924279.1"
                     /translation="YGAELVPVGDDQTQHLEFARDIAERMNSQFGDLLTVPKPVKEQH
                     AFFGKEQGLRIKDLADPTKKMSKSDETGKGIIFLGDEPEAAAKKIMSATTDSEGNIRL
                     DYDKQPGIGNLLQIQALLLDQPVEETARHWEGKSSYGELKREVAELVKDFLTDFQNRL
                     KEVDDSQLASKLAADEQAMNEVANATLYRVQQAVGLRPA"
BASE COUNT          573 a          965 c          873 g          739 t
ORIGIN      
        1 gacgtcgtct tcggaggtca cgcctggacc cgcataaaga acgccaatac tgcgtcacgg
       61 gatctggtcg aaaaccgcgg cctcgagttg caccacacaa acgacggata ctgttactac
      121 gtgtactagg agggtccgtt acacgacgac acggcggggg cgcacgatgc gcttgggccg
      181 gccggccgga acgatcagct tgttcatctt gtcggcgatg aggtcggctt cggccatcat
      241 gccacgactc tcgaacgcct tatagagaag gctgtacgtc tctttgttcg gttccagttc
      301 cgcagcccgt tccagctcct ggaacatcag cttgtcgttc ccgagctttt cttctacctt
      361 ggcataggcc acgtgccgcg ccgccagatt atcttccagc ttcagggcct gctcaaaggc
      421 gatagcggcc ttttcgtaat tctcggtctc gtaatagata agtcccaggt tatgcagact
      481 ggacggcgtc gcttccatgc tggaggcgat ctcaaaacaa tcaatggcgt ctcggaactc
      541 cttctgtttg gcatacaaaa tacccaaccg gttgtaggcg gcggcattct tttcgtcgat
      601 tttgaggatc gtcagcaagg ctttctcggc gcgcaagaag cggttctcgc gcataccttg
      661 gtgagcgata tcccacagct tacccatgcg gtcaccgact ttgcgggaga cgccggcgaa
      721 ctcatcgtcg cgcactcgag cgtgatggta cgtaagtaat ccgaagccgg ctacgatggc
      781 tatctcgaac atttctaagg cttagtatac cagattcatt acaagttaag cataaggtcg
      841 gtgagcacca gcttgggctg accgcttgtg tgcagacgct cttctgccgt gtaggcggct
      901 tgcatgatgc gctgccactg gggagtgata gcaccggccg ccaaccgaac atgggccatc
      961 tgctgcaata gctggcagac atgcaaggca aggtcgcgct gcttgcttaa gccgtcgacc
     1021 agcgccaacc gctggaagcg ggtaccttgg attatggccc gcgcctgttc agctgccgcc
     1081 gtcaactcgc tctcctcgtc acccgtcagt atcgcttcta ccaacccggg caaaccgccg
     1141 ctcataagta tggcccgctc cacggccgtg gcatcgtggc cggcagccac ataataccgc
     1201 cgtagttcgt ctttgtccgg ggtcaacaat ctgaccgtaa cggcacgtga acggactgtc
     1261 ggcaatagcg acgcagccga atcggctagc agtatgatac tggcatcggc cggcggctct
     1321 tccagcgtct tcagcaacgc attctgggct tcgcggccca taccgtgagc tgattcaata
     1381 atgaccaccc gagcaccatt gccgggcaat ttacggctca ggaactgttc gatgctccga
     1441 atttgttcaa cactgaccgc tttgctgcct tcaggtgtaa ctatgtagac ttcgcgcagg
     1501 gcgagcgata tgtcgggctt gccggtgatt tgagccgcca ggtagcgtgc caattcgcgc
     1561 ttgccgctgc ccgccgggcc ctcgagcacc gcggcgtgcg gccggctggc cagcaagcgc
     1621 tcaaccgcct cggtggtctt cgggtgtagg atgaagccgg tcatgaatca gcctcagcca
     1681 agtaatcggt gaacacctta ggtaccggcg cttcgaacac ctttcgctcc cggctcggta
     1741 gcgtcagctc cagccgctcg gcatgcagat acagccgatc agccgccagc ccaccataca
     1801 gcgtgtcgcc gacgatgggg tggccaaggt ggctcaagtg cacgcgcagt tggtgcgtcc
     1861 ggccggtctg gggccgcagt tccaacaggc tggccgcgct gttggatgaa accacctggt
     1921 attccgtcac cgacggtttg ccgttgacgc caacccggaa cgtctgcggt tttttgggat
     1981 tacgctcgat gggcatatct atgaccgcgt ggtcttccgg caaatgaccg ctgaccaccg
     2041 ctgcataggt ttttttggtc cgccgttggc tgaattgctt ctgcaaccag ctgagggctt
     2101 ccggtgtctt ggcacagatc atcacgccgc tggtcgcgcg gtccaggcgg tggacgatgc
     2161 cggccctggc attgccggct gcatcggtgg tgccttgcca ctcacccttc gccaagcggc
     2221 gtcgcagcca tgtcgcaacc gttgcctccg ggttaaaggc gcctttgctg tgtgtcagga
     2281 cgccgatggg cttgttgatg accacgcagt cgtcatcctc gtataaaatc ggcagctcga
     2341 tgtccggtat ggcctgtgct tcttcgggcc ggtagtcaac ctccacagca tcgccttcgc
     2401 gcagcttgta gccggccttg gtttctactt ccccattcac cgtcactttg ccctgctcga
     2461 ccaatttggc ggcgtaggcc cggctcagct ccggtaacag ctcgacgacc ttttgatcca
     2521 gccgttgcgc ccgtgatatc tctaaattgc tcatgccggt cggagaccga ctgcctgctg
     2581 cacacggtac agcgtggcgt tggccacttc gttcatcgct tgttcatcgg ccgcaagttt
     2641 ggaagccagc tgactgtcat ctacttcttt cagacggttt tggaagtctg tcaggaagtc
     2701 tttgactagc tcggctacct cgcgcttcag ctcgccgtaa ctgctcttac cctcccagtg
     2761 acgggcggtt tcctcgaccg gttgatcgag cagcaatgcc tggatctgca acagattgcc
     2821 gatgcccggc tgcttgtcat aatcgagtct gatattccct tcgctgtcgg tagtggcgct
     2881 cataatcttc ttggcggcgg cttccggctc atctccgagg aatataatcc ccttgcccgt
     2941 ctcatcgctc ttgctcatct tcttggtcgg atcggccaga tctttaatcc gcagcccctg
     3001 ttccttgcca aaaaaggcgt gctgttcctt aaccggcttg ggtacggtaa gcaggtcgcc
     3061 gaactgacta ttcatccgct ccgcaatatc gcgtgcgaat tcaagatgct gcgtctgatc
     3121 atcccctacc ggtactagtt ccgctccata
//
LOCUS       DASSFD010000020         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_174161, whole genome shotgun sequence.
ACCESSION   DASSFD010000020 DASSFD010000000
VERSION     DASSFD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174161"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFH39_00425"
     CDS             <1..845
                     /locus_tag="VFH39_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6924280.1"
                     /translation="ARLHRANTVPGRHDDIEGISNLDKAIIIDQSPIGRTPRSNPATY
                     TGLFTPIRELFASTPEAKLRGYSAGRFSFNVKGGRCENCAGDGIIKIEMHFLPDVYVP
                     CEVCHGKRYNREALEIHYKGKDISDVLDMTCEQGLEFFENIPAIARKLQTLVDVGLGY
                     IKLGQPATTLSGGEAQRIKLSTELSRRATGRTLYILDEPTTGLHMADVDKLLHMLHAL
                     VEAGNSMVVIEHNLDVIKNADHIIDMGPEGGSAGGQVIAEGTPEQVAKNPKSYTGQFL
                     KRML"
     gene            complement(876..1571)
                     /locus_tag="VFH39_00430"
     CDS             complement(876..1571)
                     /locus_tag="VFH39_00430"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6924281.1"
                     /translation="MLSLLHVHPLLLAQLFNVTHLIQTGGLILIALIIFGESGMMIGF
                     LFPGDTLLISAGVLAAGHHINLLHVIAVVAVAAIVGDNVGYQIGKSAGPHLFTRQDGF
                     VFRRDHIHRAHRFFEKYGSWTMLLAHFVPIVRSFAPVVAGAADMPRKLFFVFDAIGDI
                     AWAIIITLIGYFLGRQFPQVVNLIDPVLIGIIIIFLVPTLWHVLRDPKFRAAMKRRRQ
                     PKTGRSDDETTAP"
     gene            1646..2395
                     /locus_tag="VFH39_00435"
     CDS             1646..2395
                     /locus_tag="VFH39_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET6924282.1"
                     /translation="MSQDAITLTVDAREVTGKAVKHLRRDGLVPAVIHDHGKESVLVQ
                     GQYLELFRVYTRAGKHHPIELKAPAGSYTVLIKDATFEPRKNQLTHLVFNAVDKNQKV
                     EAEVPIHPRYADEENQSSPAERASLIVLPQLETIQVKAIPSKIPDFVAYDAEKLVEVG
                     DHVTVADLIVPADVEVLTEPEHAVAAVFEPAALAAANDAAGGDAEVADASETEAEHGS
                     DTDQEAQEDEIRPGGKEQKESHDQGTNPEKE"
     gene            complement(2564..>3164)
                     /locus_tag="VFH39_00440"
     CDS             complement(2564..>3164)
                     /locus_tag="VFH39_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6924283.1"
                     /translation="RLLLRFSDIQSGTIRIDGQDISKITQDDLRRSIAYVPQEPLLFH
                     RSISENIRYGNPEADQQTIEAVARMAHAHEFIATLPQGYETLVGERGVKLSGGQRQRI
                     AIARAMLKNAPILLLDEATSALDSESEVLIQEALWKLMEGRTAVVIAHRLSTIQKMDR
                     IIVLDGGKIIEEGSHKDLLQLHGTYAKLWAHQSGGFMDE"
BASE COUNT          694 a          936 c          963 g          571 t
ORIGIN      
        1 tggcgcggct gcaccgggcg aatacggtgc cgggacgtca cgatgacatc gagggcatca
       61 gcaacctcga caaggccatc atcatagatc aaagccccat cggccgcacc ccacgcagca
      121 acccggcgac atacaccggt ctgttcacac ccatccgcga gctgttcgcc agtacgcccg
      181 aggccaagct gcgcggctat agcgccgggc gtttcagctt caatgtcaaa ggcggccgct
      241 gcgaaaactg cgccggcgac ggcatcatca agatcgagat gcacttcttg ccggatgtgt
      301 acgtgccgtg cgaagtctgc cacggcaaac gctacaatcg tgaagcgctg gaaatccact
      361 acaagggcaa ggacatcagc gatgtgctcg atatgacctg tgagcagggg ctggaattct
      421 tcgagaacat tccggctatc gcccgcaaac tgcagacgct ggtagatgtg gggctgggct
      481 acataaaact cggccaaccg gccacgacgc tcagcggcgg tgaggcccag cgcatcaagc
      541 tcagtaccga actgagccgc cgggcgaccg gccgcacgct gtacatcctg gatgagccga
      601 cgacgggctt acacatggcg gatgtcgaca aactgctgca catgctgcat gcactggttg
      661 aggccggcaa cagcatggtg gtcatcgagc acaacctgga cgtcatcaag aacgccgatc
      721 atatcatcga catggggccg gaaggcggca gcgccggcgg ccaggtcatt gccgaaggca
      781 caccggagca agtcgccaag aatcccaaga gctacaccgg tcagttcttg aagcggatgc
      841 tttaaagcgc cattatcact tgtagcaggc cagcgctatg gagcggtggt ttcgtcgtcg
      901 gagcgcccgg tcttgggctg ccggcggcgc ttcatggcgg cgcggaactt cggatcgcgc
      961 agcacgtgcc ataaggtggg gaccaggaaa atgatgatga tgccgatgag caccggatcg
     1021 ataagattga cgacttgcgg gaactgccgg ccgaggaagt agccgatcag ggtgatgatt
     1081 atcgcccagg cgatatcgcc gatggcatcg aacacgaaga acagcttgcg cggcatgtcg
     1141 gcggccccgg caactaccgg cgcaaaactg cgcactatcg gcacgaaatg cgccagcagc
     1201 atcgtccagc tgccgtattt ctcgaagaag cgatgggcgc ggtgaatgtg gtcgcggcgg
     1261 aacacgaagc cgtcctgccg tgtgaagagg tgcggtccgg cgcttttgcc gatctggtag
     1321 ccgacattgt cgccgacgat ggcagcgaca gccaccactg ctatgacgtg cagaagattt
     1381 atgtgatggc cggctgccag gacaccggca ctgatgagca gcgtgtcgcc gggaaacaaa
     1441 aagccgatca tcatgcccga ctcaccgaag atgataaggg cgattaagat caggccgccg
     1501 gtctgaataa gatgcgtgac attgaacagc tgcgccagca gcaggggatg aacgtgtaga
     1561 agactaagca ttaccctcca gcttagcata gccaccccta atctggtata ctggtaagac
     1621 gattatatag aggagtgtac ccataatgtc acaagacgct atcacgttga ctgtcgatgc
     1681 ccgtgaagtc accggcaaag cggtcaagca cttacgccgc gacgggctcg tccccgccgt
     1741 cattcacgat cacggtaagg aatccgtact ggtccaaggg cagtacctgg agctgttccg
     1801 ggtgtacacc cgcgccggca aacaccaccc catcgagctg aaggcgccgg ccggcagcta
     1861 cacggtactc atcaaggacg caacctttga gccgcgcaag aaccagctga cacacctggt
     1921 gttcaacgcc gtcgacaaga accagaaggt tgaggccgaa gtgccgattc acccgcgtta
     1981 tgccgacgag gaaaaccagt cgtccccggc cgagcgggcc agccttatcg tgttgccgca
     2041 gctggagacc atccaggtca aggctatccc gagcaaaatc cctgacttcg tggcatatga
     2101 tgccgagaaa ctggtcgaag tcggtgacca cgtcaccgtc gccgatctga ttgtacctgc
     2161 cgatgtcgaa gtcctgaccg agccggagca cgctgtggcc gccgtcttcg agccggccgc
     2221 gctggccgcc gccaatgacg ctgccggtgg tgatgccgaa gtggctgacg cctccgagac
     2281 cgaagctgag cacggcagcg acaccgacca ggaggcgcag gaggacgaaa tccggccggg
     2341 tggcaaggag cagaaggaat ctcacgacca gggaaccaac cccgaaaaag aatgattttc
     2401 tggagcggcg cattgcggct aacctgctgc gcgccgctcc tcacgtacat ttagtacgct
     2461 gcggtgcggg gctcccagct taaccacact gcaccacttc agaaaaatca ttagcctaaa
     2521 agagcccgca agccgcgggc tcttttaggg ccagcatcac gggttattcg tccatgaagc
     2581 cgccggactg gtgggcccac agctttgcgt aggtaccgtg caactgcagc agatctttgt
     2641 gggaaccttc ttcgatgatc ttgccgccgt cgaggacgat gatcctgtcc atcttctgga
     2701 tggtactcag acggtgtgcg atgaccaccg ccgtgcggcc ttccatcagc ttccacagcg
     2761 cttcctgaat caacacttcg ctttcgctgt ccagggcgct ggtggcttcg tcgagcagca
     2821 gtatgggcgc gttcttcagc atggcgcgcg ctatggcgat gcgctgccgt tggccgccgc
     2881 ttaacttcac gccgcgttca ccgacgagcg tctcgtagcc ttggggcagg gtagcgatga
     2941 attcatgggc gtgcgccatc cgtgcgactg cctcgatcgt ctgctggtcg gcttccgggt
     3001 tgccgtagcg gatgttctcg ctgatgctgc ggtggaacag cagtggttcc tgcggtacgt
     3061 aggcgatgct gcggcgcagg tcgtcctggg tgattttcga aatgtcctgg ccgtcgatgc
     3121 gtatggtacc gctctggatg tccgaaaagc gcaacaaaag ccgc
//
LOCUS       DASSFD010000021         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_852522, whole genome shotgun sequence.
ACCESSION   DASSFD010000021 DASSFD010000000
VERSION     DASSFD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852522"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFH39_00445"
     CDS             complement(<1..388)
                     /locus_tag="VFH39_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924284.1"
                     /translation="MKHSNYDLYKPKKLIEDVANPSSSYFKAKDISVTPLTKLGDAYE
                     THKARKDGRIPALSNKHKPNRLWLRDLLERRKNITVRSGGGQKRIKIRHAMQRSQRRS
                     ADSSQPSSSAPLARPVSRTAAAYAAAA"
     gene            802..1497
                     /locus_tag="VFH39_00450"
     CDS             802..1497
                     /locus_tag="VFH39_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET6924285.1"
                     /translation="MRKLFITVQMILTLTVSGAFLGLASQTVSAGQQSYASSPAHRTA
                     AVQAAQQSMNVTIQNFAFSPANLTVPQGTAVTWTNKDSTAHTVTSDSGGPFVLNSGTI
                     EPGLTFSTTLNKTGTFPYHCSIHPNMKGTITVTSASSTNSGQGSKGAGGASASSGSAS
                     SSSSSSSQESGGGNSSSSTQMSGMTASGPVAAGSGSVATASHRTFDRVLVIGFAAFIA
                     AAILMIVRRLFLA"
     gene            1511..2173
                     /locus_tag="VFH39_00455"
     CDS             1511..2173
                     /locus_tag="VFH39_00455"
                     /inference="COORDINATES: protein motif:HMM:NF033748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET6924286.1"
                     /translation="MNPSAVRDRNRTLLRRLVDTFTVLLVIIGFVAVGYVLLHVGRSP
                     LPQPTPASDYATTGVTAPPRSALPSSLPTRLTIPAIGAQSALTEVGLNADGTPGVPSG
                     ATVDEAAWLKTSVTPGQTGTSIIVGHVDTLKSGSSVFYRIGRLAPGTLIRVQRQDGKT
                     AVFTVTAVRSYDKTAFPSALVYGQSGTPVLRLITCSGDWDAQSHQYTQNLVVFSSLTS
                     VE"
     gene            complement(2233..2439)
                     /locus_tag="VFH39_00460"
     CDS             complement(2233..2439)
                     /locus_tag="VFH39_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924287.1"
                     /translation="MFEFASILGFGVFAFILLVIVWVALAFLPASIAHNKGHSFIGWF
                     IISLFFWWITLFVALFLPNRNQPA"
     gene            complement(2572..>2855)
                     /locus_tag="VFH39_00465"
     CDS             complement(2572..>2855)
                     /locus_tag="VFH39_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924288.1"
                     /translation="EIAVNTIATIRLLAAGMDEVKLLADYTDSGEMDDGAIQKGFDAL
                     ELLPLDKVAVIGASPSLKKLITAMARAAGKGKIIYFAKDRADGIAWLNK"
BASE COUNT          627 a          812 c          752 g          664 t
ORIGIN      
        1 cggccgcggc cgcataagcc gctgccgtcc gtgatacagg acgagcgagc ggcgcgcttg
       61 acgacggctg ggaagaatcg gcggagcgtc tctggcttcg ctgcatggcg tgccgtatct
      121 ttatgcgctt ctggcctccc ccgctccgta cggttatatt tttgcgacgt tccagcagat
      181 cgcgtagcca caaccggttg ggcttatgct tgttggacag cgccggtata cgtccgtcct
      241 ttcgggcctt atgagtctcg tacgcgtcac cgagtttcgt cagtggtgtc acgctgatgt
      301 ctttcgcctt aaagtaggag ctggaggggt tggccacatc ctcgataagt ttctttggct
      361 tatacagatc gtaattacta tgtttcatga gtaaaaccgc tcggttaatt tcctttcgtt
      421 ggttgctatg cccgccacac gaaaagagcg gttgcatata tcaagccgct cttctaaggc
      481 tggaggttag ttgacgtatt ttctcgtcgt gtttggtttt cttgataaca gtcagccatg
      541 ccgtcaggct ggcttgctgg caaaaaagac tgagggtcga ataaggggtt gtgccggtta
      601 ggcaccgaat ggagatacgg atttacggtc acgcaggtat agcttcatta tactcccgcc
      661 agcttgcgag ggccagaaaa tgcaaatgct tttatctcaa agttatggcg ttgtgttcga
      721 cgtcacgaaa tgggcgcaac ttatcttact gaagttacgt aaggtgcttt ctacaatctt
      781 tggcataacg gggattatgg tatgagaaag ctatttatta cagtacaaat gattctgacg
      841 ttgactgtca gcggtgcatt cttggggctc gcatcacaga ccgtctcagc aggacaacag
      901 tcctacgcga gcagcccggc gcaccggaca gctgcggtac aagcagccca gcaaagcatg
      961 aacgtgacta ttcagaactt tgcgttctcg ccggccaacc taacggttcc gcagggcacg
     1021 gctgttacct ggaccaataa agatagtacg gcccatacag tgacgtcgga ttcaggaggg
     1081 ccgttcgtgc tcaatagtgg cactattgaa ccgggcttga cgttcagcac tacgctaaac
     1141 aagactggta cgttcccgta tcactgcagc attcacccaa atatgaaagg cacgattact
     1201 gttaccagtg ccagctcgac taactcaggt caaggtagca aaggtgccgg cggagcgtct
     1261 gctagctcgg ggagcgccag cagcagctct tcgagcagta gtcaggagtc aggagggggc
     1321 aacagttctt cctccacaca aatgagcggc atgacagcta gcggaccggt agcggccggc
     1381 agcgggtctg tggctacagc cagccaccgt acctttgacc gggtcttggt gatcggcttc
     1441 gccgccttca tcgccgccgc catcctgatg atcgttcgcc ggctgttcct ggcctagatc
     1501 tggctagcta atgaatccat ccgctgtcag ggatagaaat cggacgcttc tcagacggct
     1561 ggtcgatact ttcaccgtat tgctggtcat catagggttc gtagccgtcg gctatgtcct
     1621 tctccatgtg ggcaggtcgc ctttgccgca accgacgcca gccagcgact acgcaacgac
     1681 cggtgtcaca gcgccgcctc ggagcgctct gccgtcttcc ctgccaacgc gtctgacgat
     1741 tccggctatc ggggcccaat cggcacttac tgaagtcggg ttgaatgccg acggcacacc
     1801 cggggtgccc tccggtgcca ccgtggacga ggccgcctgg ctcaagacat cggtaacccc
     1861 gggccagacc ggtacctcga tcatcgtcgg gcatgtcgat accctcaaaa gcggttcctc
     1921 ggtattctat cggatcggcc gcctggcgcc tggcaccctc atacgggtgc aacgccagga
     1981 cggcaaaaca gccgtcttta ctgttactgc cgtccgctcc tacgacaaga cggcttttcc
     2041 atctgctctc gtctacggac agtcagggac gccggtgctg cgcctcatca cctgctctgg
     2101 cgactgggac gcccagagcc atcagtacac gcaaaacctg gtggttttct cgtccttgac
     2161 cagcgtggaa tagacgattg cgccacccct gcgcggtctc tgcaacggtc acaatccggc
     2221 tacatggcct gcttatgcgg gctggttacg gtttggcaaa aacagcgcca cgaacagcgt
     2281 aatccaccag aagaacaggc ttattatgaa ccagccaata aaactgtgac ctttgttgtg
     2341 ggcaatcgaa gccggcaaaa aagccagcgc cacccacact atcaccaaca gtatgaatgc
     2401 aaaaaccccg aaccctaaaa tgctcgcaaa ctcaaacata tgaagctcct tgcctacatg
     2461 cacctagact aaatgagctg actttgactt tcagtgaagc gggtcacgct gcttcctgcc
     2521 agccttctcg tgccattagc tagaagtcag tcgctagtcc ccgagccgag cctacttatt
     2581 caaccaagca ataccatccg cccggtcctt tgcgaaataa atgattttgc ccttaccggc
     2641 tgcacgagcc atggcggtaa tcaatttctt gagggaaggt gaagccccga tgaccgccac
     2701 tttatcgagt ggtagcaact ctaaggcatc aaagcctttt tgtatcgccc cgtcgtccat
     2761 ttcaccggag tctgtataat cggccagaag cttaacttca tccatcccgg cagctaataa
     2821 gcgaatggta gctatagtat ttacggctat ttccc
//
LOCUS       DASSFD010000022         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_549760, whole genome shotgun sequence.
ACCESSION   DASSFD010000022 DASSFD010000000
VERSION     DASSFD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549760"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFH39_00470"
     CDS             complement(<1..315)
                     /locus_tag="VFH39_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HET6924289.1"
                     /translation="MADQQTIIEVEDMITVGTLAERLSIPVTRLIGELMKNGVMATVN
                     ERIDFETAQIIVEELGLDFTLQKQQAPEAVIPVREKAQLSDHAQQRPPVVAVMGHVDH
                     GKT"
     gene            complement(366..1433)
                     /locus_tag="VFH39_00475"
     CDS             complement(366..1433)
                     /locus_tag="VFH39_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET6924290.1"
                     /translation="MPNNSTITMDELLASSEVKPLAASDVVEGTVSSIRKHEVWIDLG
                     ARGVGVVMRREIGHGQQLEDGQTVTVSVIDPEMDEGYALLSMKRAAKDRGWDELQRMF
                     EANEIVEVVPYDANRGGLLVELEGIRGFLPVSQLAAGHYPRVSGADKDEILQKLNQLT
                     NQPMRVRILDVSRKDNKLIFSEKEAVKDDMAARFAELKVGDVVEGIVTGVIDFGAFVN
                     VDGIEGLIHISEISWERVDNPRNYVKVGETVKAKIIAIDKDRLSLSLKQMSEDPWLNE
                     VKAFSKGDNVEGKITRITPFGAFVQLSPSVEALVHVSEMGGDDNVDPEKVFQLNEVKK
                     FKVLDIDTDNRKIALSLKDAK"
     gene            1768..>2482
                     /locus_tag="VFH39_00480"
     CDS             1768..>2482
                     /locus_tag="VFH39_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6924291.1"
                     /translation="MYAGVDIGGTKVLAAVMDEHGQITKRTKFPTPQKYEDFLAELKK
                     TFHDFHVPEFQAAGVGMPATSMDRAEGRGLTFGNLPWRNVPIQGDLEQLLRCPVAIEN
                     DAKLGALSEYMLVKDKFHKVLYVTISTGIGVGLVDNGHIDTEVGDAGGKIMYIEHNNK
                     LVAWESLASGHAIVERFGKKAMDITDEATWKLIAHDISRGLIELVAITEPEVVVIGGS
                     VGTYFDRYAKYLEAALKKFE"
BASE COUNT          491 a          769 c          709 g          513 t
ORIGIN      
        1 cgtcttaccg tggtcgacgt gacccatcac tgccaccacc ggcggccgct gctgggcgtg
       61 gtcggaaagc tgcgccttct cgcgcaccgg gatgacggct tccggcgcct gctgtttctg
      121 gagcgtgaag tccaggccta attcttcgac gataatctga gcggtttcga aatcgatccg
      181 ctcgttcaca gttgccatga ccccgttctt catcagctca ccgatgagac gggttacagg
      241 tatactcaac cgctcggcca gagtgccgac ggtgatcatg tcttcaactt cgattatggt
      301 ctgttggtct gccatcgccg acactccttt ctgcccccga aagggcaatt aaccttacga
      361 tttcattact tagcgtcttt gagtgaaagc gctatcttgc ggttgtcggt gtcgatgtcc
      421 aacaccttga acttcttgac ctcgttgagc tggaacacct tctccgggtc gacgttgtcg
      481 tcgccgccca tctcagaaac gtgcaccagg gcctcgacgc tcgggctgag ctgcacgaag
      541 gcgccgaacg gcgtgatgcg ggtgatctta ccctcgacgt tgtcgccctt gctgaaggcc
      601 ttgacctcgt tcagccacgg gtcctcgctc atctgcttga ggctcaggct caggcggtct
      661 ttgtcgatgg cgatgatctt cgccttgacg gtctcaccga ccttgacgta gttgcgcggg
      721 ttgtcgacgc gctcccagct gatctcgctg atgtggatca gcccctcgat gccgtcgacg
      781 ttgacgaacg caccgaagtc gataacaccg gtgacaatgc cttcgaccac atcaccgacc
      841 ttcagttcgg cgaagcgggc ggccatgtcg tccttgacgg cttccttctc ggaaaagatc
      901 agcttgttgt ccttgcggct gacgtccagg atgcgtacgc gcatcggctg gttagtcagc
      961 tggttcagct tctgcaggat ctcgtccttg tcggcaccgc tcacccgtgg gtaatggccg
     1021 gctgccagtt gggataccgg caggaagccg cggatacctt ccagctccac cagtaaacca
     1081 ccgcggttgg cgtcgtatgg caccacctcg acgatctcgt tggcttcgaa catgcgctgc
     1141 agctcgtccc agccgcggtc cttggcagcc cgcttcatgg aaagcagcgc gtagccctcg
     1201 tccatctctg gatcgatgac gctgacggtg acggtctgac cgtcctccag ctgttggccg
     1261 tggccgattt cgcggcgcat gaccactccg acgccccggg cacccaggtc gatccagacc
     1321 tcgtgcttcc gaatggagct gacggttccc tcgacgacgt cactcgccgc caggggtttg
     1381 acttcgctgc tcgcgagcag ctcgtccatg gtaatagtac tgttgtttgg cattttcatg
     1441 cctcctcttc cgtcccggtt cttcacgccg gaactagtgt atttcttcag acaaagcacc
     1501 tctagcaaga agctacaggt cccccatctg taagattact gtaagtatac caaagaaacg
     1561 gaggtgaagc aaaacgccga aagaggcggt cgacttcttg ggattcagat gaatccaaaa
     1621 taagtacgaa cgccgtaggc cggcgcagtt gcggatgaca ggcgagcacc gaagcgcagc
     1681 gtacattgag gtacgagagc atcggagcac agccaggcgc cgcaaatgtg ctggtctacg
     1741 gcgttcgcca gatgctacac tacacatatg tacgcggggg tcgacatagg gggtaccaag
     1801 gtgctggcag cagtcatgga cgagcacggc cagattacca agcgcaccaa gttccccacc
     1861 ccgcagaagt acgaggattt cctcgcggag ctgaagaaga ccttccacga tttccacgtc
     1921 ccggaattcc aggccgccgg cgtcggtatg ccggccacca gcatggaccg cgccgaaggc
     1981 cgcggtctga ccttcggcaa cctgccctgg cgcaacgtcc ccatccaggg cgacctcgag
     2041 cagctgctgc gctgcccggt ggccatcgag aacgatgcca agctcggggc actgagcgaa
     2101 tacatgctcg taaaagataa gttccacaag gtgttatacg tcaccatcag taccggcatc
     2161 ggcgtcggcc tggtagataa cggccacatc gacaccgaag tcggcgacgc cggcggcaag
     2221 atcatgtaca tcgagcacaa caacaagctg gtggcgtggg agagcctggc cagcggccac
     2281 gccatcgtcg agcgcttcgg caaaaaggcc atggacatca ccgacgaggc cacctggaag
     2341 ctcatcgccc acgacatctc ccgtgggctc atcgaactgg tggccataac cgagccggag
     2401 gtggtcgtca tcggcggcag cgtcggcacg tacttcgacc gctacgccaa gtacctcgag
     2461 gcggcgctga agaagtttga ga
//
LOCUS       DASSFD010000023         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1310227, whole genome shotgun sequence.
ACCESSION   DASSFD010000023 DASSFD010000000
VERSION     DASSFD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310227"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..973
                     /locus_tag="VFH39_00485"
     CDS             200..973
                     /locus_tag="VFH39_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924292.1"
                     /translation="MVETRRNPMHNEWDAQRRLSDAELIRGGAAFVGHLAVHAARCHE
                     GLLAVNEYQWEKARLVRDVSFRVATELGLDLQSNEWRALFENTSLDEPLAHHWYENHP
                     HDDARVPRILDREGIDTVRDLLVIGRQKTQAIRSFGPKTVDKLAVIVKGEAPEVEWLD
                     EPSVQDIASFCESIYQVNAKVLVDGRESFPSRLSVRDVLVLPEADRTEVLADSKRYWP
                     DYLREEFARDVYARAELFALRFDLAKGGAWTNPKYLATD"
     gene            complement(990..1532)
                     /locus_tag="VFH39_00490"
     CDS             complement(990..1532)
                     /locus_tag="VFH39_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET6924293.1"
                     /translation="MTKYAAMLRGIGPTNPNMRGEKLCEVFTSLGFANVQSVITSGNV
                     LFESDIADTAKLEAMVEAALPRQLSFSSTTFIRSQAELQALVDADPFPGVEQGPQHYI
                     TMTFLKTQPQDGPTLPYRPANGGYEVLALYDRAISATLDQTREKTPNLMAWLEKNYGK
                     NITTRTWKTVNKLLDKLNEA"
     gene            1600..2802
                     /locus_tag="VFH39_00495"
     CDS             1600..2802
                     /locus_tag="VFH39_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924294.1"
                     /translation="MRRFIRLHPRQFAAIMAVVGIGAIGSALLLSSHAQTPVVSKEAE
                     NGTVNSPATTIADKSASGGQAIKFNKPTSPPPTGNCAVTAILVNPCGPWLGAWANFYP
                     GHKNTGAKDEILNHEARIGRQIQMAKDYLNYGGTLTADAKYFVDRPNTILVTTWKAAP
                     SGNWAAAGGGNATVNANIDAMAKSIKSVAPHKVVLIVWHEPENDVTASTTSCSGAGTT
                     NTSGSPADYVNMWHNIRARFDALGVNNVVWGMNYMLYNGGTCLIKPLWPGNNYVDWVF
                     TDPYFNAPNTNYTSRIDFTYDWLKNNSDATHDFNSKPWGLAEWGVHPTTDTLKCQAFA
                     DAKTDLDNNRYPNLKAYIIFDSSAGANGGGIVGLSNDGTTIDTVTQNCYNKFANDPLF
                     SKPFTGLP"
     gene            2899..>3841
                     /locus_tag="VFH39_00500"
     CDS             2899..>3841
                     /locus_tag="VFH39_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924295.1"
                     /translation="MIVNKQVMKIVHHVNRNAKAYLMVALVFGTAGTLLLGGSHALTP
                     TANIEAEAGTATGATAVSDSSASGGSAIRFHQTCTVSATLVNSCRPWLGAAANNYPGI
                     TGLANRIADHESRIGRQLDIVHDYLNPGAVLSSDDVTLATRANTILLVNWKPSLTWST
                     AGGSDSTVNTQIDNMANSIKALGSTKVMLTIFHEPENDVSTGGDPNCTSITYQGSAGT
                     VTDYVNMWHNVRSRFDADGVTNVVWVMNYMGYPTWNCLVPDLWPGNSYVDWIMWDPYS
                     QSSDFSGMVNTFYSYLTTNSDSTHDYTSKPWGLAEWGN"
BASE COUNT          821 a         1154 c         1147 g          719 t
ORIGIN      
        1 ccttgaccgc gactacctga tagcagtcgg caacacgagg cgtcggacat gggcggccca
       61 ccagtttgcg gtcaccgcgt atgtattgag taaggcagtg caaagttagt acggtgtcgt
      121 attatgcaaa aagccctccg gaaacggagg tctttttctt tacatacgca agctgaagac
      181 ctctggtaga atgggccgca tggttgaaac ccggaggaac ccgatgcata acgaatggga
      241 tgcgcagcgg cggttgagcg acgctgaact tattcgtggc ggggcggcct tcgtcgggca
      301 cctggcagtg cacgccgccc ggtgccatga aggcctgctg gccgtcaacg aatatcaatg
      361 ggaaaaggct cggctggtgc gcgacgtgtc gttccgggta gctactgagc tgggccttga
      421 tctgcagtcg aacgaatggc gggcactgtt cgagaacacg tctctggatg agccgttggc
      481 acatcactgg tacgagaatc atccccatga tgacgcccga gtgccccgga tccttgaccg
      541 cgagggaatt gatacggtcc gtgacctgct ggtaattggg cgtcaaaaga cgcaggctat
      601 ccgcagtttc ggaccgaaga cggtcgataa gcttgcggta atcgtaaaag gggaggcacc
      661 agaggtggag tggttggacg agccgagcgt gcaggatatc gcgtcgttct gcgaatctat
      721 ctaccaggta aacgccaagg tgttagtgga tggcagggag tcgttcccgt cccgcctgag
      781 cgtgcgtgat gtgctggtgc tgcccgaggc ggatcgtacc gaagttctag ccgatagcaa
      841 gcggtactgg cccgattatc tgcgcgagga gttcgcccgc gatgtgtacg cccgtgcgga
      901 gctgttcgcc ctgcggttcg acctggcaaa gggcggcgcc tggaccaatc ccaagtacct
      961 ggctaccgac taaatccggc gcgcacatat tacgcttcgt tcagcttgtc caggagctta
     1021 ttcaccgtct tccaggtgcg ggtggtgatg tttttgccgt agtttttctc gagccatgcc
     1081 atgaggttcg gtgttttttc gcgcgtttgg tcgagcgtgg cgcttatggc gcggtcgtag
     1141 agggcgagga cttcgtagcc gccgtttgca ggccggtagg gcagcgtggg gccgtcctgc
     1201 ggctgcgttt tgaggaatgt catcgtgatg taatgctgcg ggccttgctc aacccctggg
     1261 aacggatcgg catcgaccag cgcctgcagt tcggcctgcg agcggatgaa ggtagtgctg
     1321 ctgaagctga gctggcgggg gagtgccgct tcgaccatcg cttccagctt ggcggtgtcg
     1381 gctatatcgc tctcgaacag cacgttgccg ctggtaatga cgctctggac attggcaaaa
     1441 ccgaggctcg taaacacctc acacaacttc tcgccgcgca tgttcgggtt ggtcggcccg
     1501 atgccgcgca gcatggcggc gtatttcgtc atgccaaaaa gtatagcagc tagcacgacg
     1561 gcaagagttg gtgtagagta tgggaaagac gtaagcaata tgaggcggtt catacgactt
     1621 caccccaggc agttcgcggc catcatggcc gtggtgggta tcggtgccat cggcagcgcc
     1681 ttgctgctgt cgagtcatgc gcagacgcct gtcgtctcca aagaagccga aaacggcacg
     1741 gtaaacagcc cggccacgac tatcgctgat aagtccgcct ccggcggcca ggccatcaag
     1801 ttcaacaaac ctacttcacc gccgcccacc ggcaactgcg cagtgacggc catcctggtg
     1861 aacccgtgcg ggccatggct gggcgcctgg gcgaattttt atcccggcca caaaaacacc
     1921 ggtgccaaag atgagatact aaatcacgaa gcgcgcatcg gccgtcagat ccaaatggcc
     1981 aaagactacc tgaactacgg cggtacgctg accgccgacg ccaaatactt cgtggaccgc
     2041 ccgaacacca tcctggtcac tacctggaag gcagcgccaa gcggcaactg ggccgccgcc
     2101 gggggcggca acgccacggt caatgccaac atcgacgcca tggccaaaag catcaaatcg
     2161 gtcgcgccgc acaaggtcgt ccttatcgtg tggcacgagc cggaaaacga cgtcaccgct
     2221 tccactacct cctgctccgg tgccggcacg accaacacct cggggagtcc tgccgactac
     2281 gtcaacatgt ggcacaacat ccgggcccgc ttcgacgcct tgggcgtgaa caacgtcgtg
     2341 tggggcatga actacatgct gtacaacggc ggtacgtgcc tcataaagcc gctctggccc
     2401 ggcaacaatt acgtcgactg ggtgtttacc gacccgtact tcaacgctcc caatacgaac
     2461 tacaccagcc gcatcgactt cacctacgac tggctgaaga ataacagtga cgccacgcac
     2521 gacttcaact ccaagccgtg gggattggcg gagtggggtg tgcatcccac gaccgacaca
     2581 ctgaagtgtc aggccttcgc cgacgccaag accgatctgg acaataaccg ctacccgaac
     2641 ctcaaggcct acatcatctt cgactcttcg gccggggcta acggcggtgg catagtcggg
     2701 ctgtcgaacg atggcacgac gatcgatacg gttactcaga actgctacaa caagttcgcc
     2761 aacgacccgc tgttcagcaa accgttcacc ggcttaccgt aacggacggc ctatgtggca
     2821 aagccggggc atccccgggc agagggtcct ctaggcaagg cgccgaatgc accgtacaat
     2881 agacatgaga attacgctat gatcgtaaac aaacaggtga tgaagatagt ccaccacgtc
     2941 aatcgcaacg ctaaggccta cttgatggta gcgctggttt tcggtaccgc cggcacgctg
     3001 ttgctgggcg gctcgcacgc cctgacgccg acggccaaca tcgaggccga ggccggtacc
     3061 gccaccggcg ccacggccgt atccgattcg tctgcctccg gcggttcggc gatccgcttc
     3121 catcagacct gcacggtctc ggcgacgctg gtcaactcct gccggccgtg gctcggcgcg
     3181 gcggcgaaca actaccctgg cataacgggt ctcgcgaacc gtatcgccga tcacgagagc
     3241 cgcatcgggc ggcagctcga catcgtccac gattacctca acccgggagc cgtgctgtcg
     3301 agcgatgacg tcaccctggc gacccgggcc aacaccatcc tgctggtcaa ctggaagccg
     3361 tccctgacct ggagcaccgc tggcggcagc gactcgacgg tcaacaccca gatcgacaac
     3421 atggccaaca gtatcaaggc gctcggcagc accaaggtca tgctgaccat cttccacgaa
     3481 cccgagaacg atgtttccac cggcggtgac ccgaactgca cgagcatcac ctaccagggg
     3541 tcggccggca cggtcacgga ttacgtcaac atgtggcaca acgtacgcag ccgcttcgat
     3601 gccgacggcg tcaccaacgt cgtctgggtg atgaactaca tgggctaccc tacctggaac
     3661 tgcctggtgc cggatctgtg gccaggcaac agttacgtgg actggatcat gtgggatccg
     3721 tacagccaaa gcagtgattt cagcggcatg gtcaacacct tctacagcta cctcacgacc
     3781 aacagtgaca gcacgcacga ctacacgagc aaaccgtggg gtctggccga gtggggcaat
     3841 a
//
LOCUS       DASSFD010000024         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1846979, whole genome shotgun sequence.
ACCESSION   DASSFD010000024 DASSFD010000000
VERSION     DASSFD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846979"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..429
                     /locus_tag="VFH39_00505"
     tRNA            353..429
                     /locus_tag="VFH39_00505"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:387..389,aa:Asp,seq:gtc)
     gene            1576..1902
                     /locus_tag="VFH39_00510"
     CDS             1576..1902
                     /locus_tag="VFH39_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HET6924296.1"
                     /translation="MHHFGMRHKHERRPEHKTHKKTPYVVFLDKLTFVAGVVGPFTVL
                     PQIWSIFSTHSAAGVSMATWLLIFIVTLPWVFYGVAHRDKSIIVSFTLWEVVNLTVVI
                     GVILYH"
     gene            2041..2352
                     /locus_tag="VFH39_00515"
     CDS             2041..2352
                     /locus_tag="VFH39_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924297.1"
                     /translation="MSKPTHKQLINAELATRSFNHEAKEVIKDHNPADAVKDLVLSFH
                     CECSDRNCEERVPLTLQQYEDIHRNRAHFVLAPGHEEPRVEKVKATSSSHTVVEKPAL
                     K"
     gene            complement(2370..3014)
                     /locus_tag="VFH39_00520"
     CDS             complement(2370..3014)
                     /locus_tag="VFH39_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924298.1"
                     /translation="MAHTLTHPHLGGKYHNEETDDVRRQRGHIVERDMAVARSRFGGF
                     HFGAAFFGWLVSTGLAAILIGILGAAGGALAVTSIGNVQELNATTVSTIGLWSGIVFL
                     IVLAISYYAGGYVAGRLARFDGARQGFGVWVIGVIVTIILALAGAALGAKYNVLQQIN
                     LPHIPIKQGAFTTGGWITSVLALIITLLTALAGGRAGMHYHRKVDSAAVVDREV"
     gene            complement(3137..3280)
                     /locus_tag="VFH39_00525"
     CDS             complement(3137..3280)
                     /locus_tag="VFH39_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924299.1"
                     /translation="MEDKAKNEAKDVMNNPDDRAKIEKIARDKGMSLDEAKDHFMKHG
                     DKS"
     gene            complement(3480..>3986)
                     /locus_tag="VFH39_00530"
     CDS             complement(3480..>3986)
                     /locus_tag="VFH39_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET6924300.1"
                     /translation="VAQASCLSSTDQAQRYQLLSFAISTLKANPGAKVYLDAGNPSWV
                     AASDMAGRLKQAGIANADGFSLNVSNFIATKDNETYGAQLSALVGGKHFVIDTSRNGN
                     GSNGQWCNPSGRALGAAPTASTGASLVDYFLWVKTPGESDGTCNGGPAAGSWWPSYAE
                     SLALNADW"
BASE COUNT          887 a         1135 c         1083 g          881 t
ORIGIN      
        1 acggcgctcg gctcggcatc aatgtgtatg ctggtcgtaa ccctggtttt catgtttgtt
       61 agaggttatc atcctcttta taccctataa tccgtcggtc cggcgtgaca cgcgtcacaa
      121 ctgttgaccg ccacaatatt atagcccgct ggcagcctca ttagaagcat tgcgttatgt
      181 ccctccattt ggtaaactta cccctgttgc ttacacaaat ctgcagctaa aaccaaaact
      241 tcaaatcagg gagcgaagcc gaccgtttga agttttgaga ggacgaccgt agcattaagg
      301 cgaagctttt gcccgcagag gcaaaacgag ccagacgcgt acggaaggac gagggctcgt
      361 agtgaagagg cctatcacgc ctccctgtca cggaggagat cgccggttcg aatccggtcg
      421 ggcccgccaa acaaagggac tcatcgcaag atgggtcttt ttgtttgacc tgccccagtg
      481 atgtgagtcg gcgatcagcg aatgcagtga gcgtaggtcg ccgattcgat gacaagcgcc
      541 acgcgcagga gctcgctctg agcatggcgc gccggcagta gcgaagcgtc aaccggtcgg
      601 gcccgccaag aaaaatgtat ctgccgcaag gtaggtgcat ttttcttgct ttggcgtccc
      661 tgcgatatga accgctcgaa aagtaaggtt cggtcgttag tctgctgccc gagttatact
      721 cggcggacag attaacgtgc gctacccggt caggcgcgcc aaaggagttc ccttaacacc
      781 gatcagtaag tgagataatt gactattagt tattaatatg ttattattgt attgagcaaa
      841 ctacttaagc tatgtagaat accggtaatc ccgctatgat ttcaaatcat cagaattaag
      901 tgccctccgg cacgaacata gaggccagac aatcggtttg cggcttggtt gttacgatat
      961 attgcacgct ggtcatcaac gcggcgtgga cgttgtcgga aaacacgccg atttaacggt
     1021 tattggtgtc atgcccgatg aatacgttcg gaaacacaag ggtgacgacc gcctattaat
     1081 ggcgagcaaa caagggtaga ggcaatcgat aaagccgaag gcgtagacta cagcttcata
     1141 gccgagggcg gtcgtttggc gttggcggga gtaatgctcc gcttaaggcc agacgtgtat
     1201 atcgaagatg cggagtatgg gggaagcaag gtaagagcag cgttcctatc aatcataggg
     1261 acagagtatg ttatcgacga tactcctcac cccggctcgt cgagcgctat gattgccgaa
     1321 ctaggcgcca aagaagccca agcctattca aacctaagct atatgccgat ctagcgcggc
     1381 taggctacag tttcatatca ggccgccgcg gtggtatgat gccggcttct ttctgcgcaa
     1441 acccatggct ttcttcgagg ccgagtgcaa actcgccgcg ggcgcgcaac gcattataca
     1501 tctatagatg ccctcttaat gtattctcat tctgtcgcca gggaggccgt tacgctcatg
     1561 ctatagtgga agtatatgca tcactttggc atgcggcata aacacgagcg ccgccccgaa
     1621 cacaaaaccc acaagaaaac accgtacgtg gtattcctgg acaagctgac cttcgtggcc
     1681 ggcgtggtcg ggccctttac cgtactaccg caaatctgga gcatcttctc gacgcattcc
     1741 gccgccggcg tctcgatggc gacctggctg ctgatattca tagtcaccct gccctgggtg
     1801 ttctatggcg tggcgcaccg tgacaaaagc atcatcgtca gcttcacgct gtgggaagtc
     1861 gtcaacctga ctgtggtcat cggcgtcatc ctctatcact gagcgtttct tcacgtggtt
     1921 catcggtttc cgtgacattc gccaaagccc tgattcgatt cccggaatgc cggacgtcgg
     1981 gcatctgtcg ggaagccgga tgtgctatag tttttatagt aaaactaacg tagaaacctc
     2041 atgagcaaac ctactcacaa acaactgata aacgccgaat tggcgacccg gtcattcaat
     2101 catgaggcca aagaggtcat caaagatcac aaccccgcgg acgcggtgaa ggatctcgtg
     2161 ctgtccttcc actgcgaatg ctccgatagg aactgcgagg aacgggtgcc gctgaccctt
     2221 cagcagtacg aagatataca taggaaccgg gctcatttcg tgctggcgcc gggccacgaa
     2281 gaaccacggg tcgaaaaggt caaagcgacc tcgagctcgc acaccgtcgt cgaaaagccg
     2341 gcgcttaaat agaactgtcc cgagctcacc taaacttcgc ggtcaacgac ggccgcactg
     2401 tccaccttgc ggtgatagtg cataccggcc cggcctccgg ccagtgccgt caacagcgta
     2461 atgatgaggg ccagcaccga tgttatccaa ccgcccgtcg tgaaggcgcc ctgcttaatg
     2521 ggaatatgcg gtaggtttat ctgctgcagg acgttgtact tggcccccaa ggcagctccg
     2581 gccagtgcca gaatgatagt tacgatcact ccgatcaccc agaccccgaa accctgtcgg
     2641 gcaccatcga agcgggccag ccgccccgcc acatagcccc cggcgtaata ggaaattgcc
     2701 aggacgatca ggaacacgat gccgctccac agcccaatgg tcgagacggt ggtcgcattc
     2761 agctcctgca cgttaccgat ggacgtgacg gctagcgcgc cgcccgccgc gcccaggata
     2821 ccgataagta tggcggccaa gccggtactg accaaccagc cgaagaacgc ggcaccgaag
     2881 tgaaagccgc cgaaccggct gcgcgccacc gccatgtcgc gctcgacgat gtgcccccgc
     2941 tgacggcgga cgtcatctgt ttcttcgtta tgatatttcc cacctaaatg agggtgtgta
     3001 agcgtatgtg ccatagcgga cctccttgct tatagctacc cctaattaca ctccagcgca
     3061 gcggcctgca ctatgaatat tcttactatt ttaataaacg gctcccctga gggagccgcc
     3121 ccacttgcag gccgacttac gatttatcgc cgtgcttcat gaagtgatct ttggcctcgt
     3181 ccaggctcat gcccttgtcg cgcgcgatct tctcgatctt cgcccggtcg tccgggttat
     3241 tcatgacgtc cttcgcctcg ttctttgcct tgtcttccat ttcgtccatc atgcccatag
     3301 cgcttccctc ttttacgtag ttactccagt gtacgggctg gcggcgggaa cggctgtagc
     3361 atatttgata gcgaccgccg gagcgataat cgttgcccgg ccctcaacaa ttgctaagat
     3421 atatgattgc ttatcggcag caggcaggct actctccagg cgttactagt aggcatacat
     3481 taccagtcgg cgttgagcgc caggctctcg gcatagctcg gccaccagct gcccgctgcc
     3541 gggccgccgt tgcacgtgcc atctgattca cccggagttt tgacccatag gaagtaatcc
     3601 accaggctgg cgccggtact ggccgtcggc gcggcgccca gtgccctgcc cgacgggttg
     3661 caccactggc cgttgctgcc gttgccgtta cgcgacgtat cgatgacgaa atgcttaccg
     3721 ccgacgagtg ccgatagctg cgcgccgtac gtctcgttgt ctttggtggc gatgaagttc
     3781 gaaacattca gactgaaacc gtcggcattg gcgatgccag cctgcttgag ccgcccggcc
     3841 atatcgctgg ccgcgaccca ggagggattg ccggcatcga ggtagacctt ggcgccaggg
     3901 ttggctttaa gcgtgctgat ggcgaagctc agcagctggt aacgctgtgc ttggtcagtc
     3961 gacgataggc aggatgcctg ggcgac
//
LOCUS       DASSFD010000025         6721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_733522, whole genome shotgun sequence.
ACCESSION   DASSFD010000025 DASSFD010000000
VERSION     DASSFD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733522"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFH39_00535"
     CDS             <1..405
                     /locus_tag="VFH39_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924301.1"
                     /translation="RIGYLNYKMIALDPTPIDNDTRNGLVADRLLIRRRRSLYDLRLQ
                     DVREIEVFKESFALIIKSQLPPEQREAIDHAYRNYPLAYIEDNNSDVAMIGAQIFEEA
                     IVAPAGTTTTLIPIDTAYIASMLRRARKRKAA"
     gene            586..1206
                     /gene="rplC"
                     /locus_tag="VFH39_00540"
     CDS             586..1206
                     /gene="rplC"
                     /locus_tag="VFH39_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET6924302.1"
                     /translation="MKALITRKVGMTSTVSEDGAVQAVTLLSASPCVITQVKTDDTDG
                     YTAVQLGFETAKQQNVAKAQTGHFKAAKVLPKVTREFRVSEITEDLKVGESVGPEVFS
                     VGDVVHVTGTSKGKGWAGTIKRHNFHRQRKTHGGKGNTRKVGSIGSMYPQHILKGKKM
                     AGQMGHEQVTVRNLKVAFVDAELGVIGVTGAVPGPRKSIVLVKGDD"
     gene            1206..1844
                     /gene="rplD"
                     /locus_tag="VFH39_00545"
     CDS             1206..1844
                     /gene="rplD"
                     /locus_tag="VFH39_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET6924303.1"
                     /translation="MPTPTFTKTGTKATTPAKLDKAVFGVEVKNHELLKQAYVTYLAN
                     GRENLAVAKTRGEVSGGGRKPWKQKGTGRARFGSSRNPIWTGGGVAFGPTGEENYSKR
                     LSTKAKRTALRQALSLANEAGKVIVIETFETKDGKTAPAAKLLSKVGASKNVLLVVSV
                     KDDLIERATRNLENVKAVQANYLNVFDILNADSIVVDEKSLAVISEWLGGTK"
     gene            1844..2296
                     /gene="rplW"
                     /locus_tag="VFH39_00550"
     CDS             1844..2296
                     /gene="rplW"
                     /locus_tag="VFH39_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET6924304.1"
                     /translation="MSKNVTLLPRIMSEKAYRLSHETRTYIFDIPQGLNKQSVARAVA
                     AQFDVTVTNVNIANKPGKAKRTLTKRRQAQGRDVASRRAYVTVAEGQSLPIFAAVEEA
                     EEKQEAIQEKVEKATAKQAAKEAKASGNDAKPAKKLSLKRSGKKKEEK"
     gene            2296..3153
                     /gene="rplB"
                     /locus_tag="VFH39_00555"
     CDS             2296..3153
                     /gene="rplB"
                     /locus_tag="VFH39_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET6924305.1"
                     /translation="MAIKQYNPTTPGRRGMTSQDFAGITTKKPLRSLTVIKRRGNGRN
                     NQGRITTRHQGGGAKQFYRIVNFKLDAGTEATIEHIEYDPNRSARIARIKDNKGVYHY
                     ILAAQGMKVGQKLASGPEASIEAGNRLPLKNIPVGSTIHAIELQPGKGAQMVRSAGNS
                     AQLMGRADNGYVQVRLPSGEVRLVNENCTASLGVIGNEQHQNVKIGKAGRSRHKGIRP
                     SVRGVVMNAADHPHGGGDGGRHGVGGGKNHGTAPKTPWGQKTLGYKTRRRKSTNKFIV
                     RSRHAAKRK"
     gene            3164..3433
                     /gene="rpsS"
                     /locus_tag="VFH39_00560"
     CDS             3164..3433
                     /gene="rpsS"
                     /locus_tag="VFH39_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET6924306.1"
                     /translation="MSRSLKKGPFVDFKLAKKVAALGTDDRTIIKTWARASTITPDFV
                     GRTIAVHNGKMHVPVFITENMVGHKLGEFSPTRKFRSHGGKLAKK"
     gene            3445..3837
                     /gene="rplV"
                     /locus_tag="VFH39_00565"
     CDS             3445..3837
                     /gene="rplV"
                     /locus_tag="VFH39_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET6924307.1"
                     /translation="MPVQAIAKGVSLSPRKVAVVAALVRGRSVDDALTILENTPRRSA
                     LAVSKVIASARANADHNHGYKPATLQIVEISVTSGPRLKRFRPAAHGRALRFQRRSSH
                     IRVVVDGEMREVKKPAAKPATATKKEEK"
     gene            3837..4463
                     /gene="rpsC"
                     /locus_tag="VFH39_00570"
     CDS             3837..4463
                     /gene="rpsC"
                     /locus_tag="VFH39_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET6924308.1"
                     /translation="MGQKVNPISMRLQVNKDWRSKWFVDKRDYAKYLKDDLAVRRMIQ
                     NKLGSRAAINKVDISRTPNLVTVTIATAKAGVVIGRGGSGAQELKETIEKIYGTPTRV
                     NIEEVKKSELYAKLVAENIANQLERRMSFRRAIKSAAAATMRAGAKGIRIEVAGRLNG
                     AEMSRREKETAGSVPLHTLRADIDFALVKAKTPAGIIGVKVWICKGEA"
     gene            4465..4878
                     /gene="rplP"
                     /locus_tag="VFH39_00575"
     CDS             4465..4878
                     /gene="rplP"
                     /locus_tag="VFH39_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET6924309.1"
                     /translation="MLVPKRTLHRKVRKGKIRGVATSGNTIAFGSFALQAQGHERITS
                     RQIEASRQAMTRYIKRGGKIWIRIFPHTPVTRKPLDVKMGSGKGNPEFFVAKVRPGTI
                     LFEMQGVPEETAREAMRLAAHKLPVKTKFLVREEA"
     gene            4883..5083
                     /gene="rpmC"
                     /locus_tag="VFH39_00580"
     CDS             4883..5083
                     /gene="rpmC"
                     /locus_tag="VFH39_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET6924310.1"
                     /translation="MKVTDIRKLSTADLTLESTKLREEIAELKRRVISGEQQNVRLIR
                     NKRKDLARVLTVLSEQLVKETK"
     gene            5083..5316
                     /gene="rpsQ"
                     /locus_tag="VFH39_00585"
                     /pseudo
     CDS             5083..5316
                     /gene="rpsQ"
                     /locus_tag="VFH39_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            5424..5792
                     /gene="rplN"
                     /locus_tag="VFH39_00590"
     CDS             5424..5792
                     /gene="rplN"
                     /locus_tag="VFH39_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET6924311.1"
                     /translation="MIQQETRLNVCDNSGAKEILCIRVLGGTRRRYARVGDVIVATVK
                     DANPSGNVKKKSVVKAVVVRTQEKIRRKDGSTIRFDDNAAVIIADDKNPRATRIFGPV
                     PRELRDLGYAKIISLAPEVL"
     gene            5794..6141
                     /gene="rplX"
                     /locus_tag="VFH39_00595"
     CDS             5794..6141
                     /gene="rplX"
                     /locus_tag="VFH39_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET6924312.1"
                     /translation="MSTKQTTKTTYKIRLKKGDTVVVRSGKYKGQTGKITATHPSENK
                     VTVEGINIVKKHVKPNREHPQGAIVELTKPIWVSKVAIVEPTSKKASKIGYNVAADGT
                     KTRVFKKTGKEIK"
     gene            6141..>6721
                     /gene="rplE"
                     /locus_tag="VFH39_00600"
     CDS             6141..>6721
                     /gene="rplE"
                     /locus_tag="VFH39_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET6924313.1"
                     /translation="MATATKTAKPAKQAASTARLRQDYQTRIVPELEKELGLKNVHQL
                     PKLEKIVVNVGLGKAKDDKKVIEAAKNTLTKITGQAPVETFAKKSIAGFKLREGNMIG
                     IKVTLRGDKMYEFADRLINIVLPRLRDFHGVSPKSFDKAGNYSIGMTDQSVFPELTFD
                     ETTTAHGLQAVFVIDAHEPAHARALLEKFGMPF"
BASE COUNT         1667 a         2032 c         1931 g         1091 t
ORIGIN      
        1 cgcatcggtt acctcaacta caagatgatt gcccttgacc ccacgccaat cgataatgat
       61 acccggaatg gtctcgtggc ggaccggcta ctgattcgac gtcgccgcag tttgtatgac
      121 ctacggttgc aggatgtccg agaaatcgag gtgtttaaag agtccttcgc gctgataata
      181 aagtcgcaac taccgcctga acagcgcgag gccattgacc acgcctaccg caactatcca
      241 ttggcttaca tcgaggacaa caactcggac gtcgcaatga tcggggcaca gattttcgaa
      301 gaagccatcg tcgctcctgc gggcacgacg acaaccctca tacctatcga tacagcctat
      361 attgcttcta tgctgcgcag ggcacgtaaa cgcaaagctg catagtagaa tttgcaacag
      421 gttgacaggg gtaagtttaa catgttactc tagtatggta tttatcaaga tttcgtactt
      481 ggtgagtcac gcaggtctac caaaaacaga ggtagaaacc actggcaacc aagcattttt
      541 atgctcaggc ccacggccgg tacgacgtaa caagtaaggt gaactatgaa agctctcatt
      601 acgcgaaagg tcggcatgac cagcaccgtt tccgaagacg gcgctgtgca ggctgtgacc
      661 ttgctttccg ctagtccctg cgtcatcacg caggtgaaga ccgatgacac tgacggctac
      721 acggccgtgc agctcggttt cgaaaccgcc aagcagcaga acgttgccaa ggcgcaaacc
      781 ggccacttca aggccgccaa ggtactcccg aaggtaaccc gtgagttccg cgtatccgaa
      841 ataaccgaag acctcaaggt tggcgaaagt gtcggccccg aggtcttttc ggttggcgac
      901 gtggtgcacg tgaccgggac cagcaagggt aagggttggg ccggtaccat caagcggcac
      961 aacttccacc gccagcgcaa aacgcacggt ggcaagggca acactcgcaa agtcggttcc
     1021 atcggttcga tgtacccgca gcatatttta aagggcaaga agatggccgg tcagatgggt
     1081 cacgaacagg ttaccgtccg taacctcaag gtcgcctttg tggacgccga gctcggcgtc
     1141 atcggggtga ccggtgccgt tcctggcccg cgcaagagca tcgtgctcgt gaaaggagac
     1201 gactaatgcc tactcctacc tttactaaga ccggtaccaa ggcaaccacg ccggccaaac
     1261 tcgacaaagc agttttcggt gtcgaggtca agaatcacga actgctgaag caggcatatg
     1321 tcacctatct ggccaatggc cgcgaaaacc tggcggtcgc caagacccgt ggtgaagtca
     1381 gcggcggtgg gcgtaagccg tggaagcaga agggcaccgg ccgcgcccgt ttcggctcca
     1441 gccgtaaccc gatctggacc ggtggtggcg tcgcgttcgg cccgaccggc gaagagaact
     1501 acagcaagcg tctgagcacc aaggccaagc gcaccgcgct gcgccaggcg ctgagccttg
     1561 ctaacgaagc cggcaaggtc atcgttatcg agaccttcga gaccaaggat ggcaagaccg
     1621 ctccggccgc caagctgctg agcaaagtcg gcgccagcaa gaacgtgttg ctcgtagtca
     1681 gcgtcaagga cgacctcatt gagcgcgcta cccgcaacct ggagaatgtc aaagccgtac
     1741 aggccaacta tctgaatgtc ttcgacatcc tgaacgccga cagtatcgtg gtagacgaga
     1801 agtcattggc tgtcatcagc gaatggctgg gaggtaccaa gtaatgtcca agaacgttac
     1861 cttactgccg cgcatcatga gcgagaaggc ctaccgcctg agccatgaga cgcgtaccta
     1921 tattttcgat atcccgcagg gcctgaacaa gcagagcgtc gcccgggccg tggctgccca
     1981 attcgacgtc acggttacga acgtgaatat cgccaacaag cccggtaagg ccaagcggac
     2041 gctgaccaag cggcgccagg cgcaaggccg ggacgttgcc agccgtcggg cttacgtgac
     2101 cgttgccgag ggccagagcc tgccgatctt cgccgccgtc gaggaagccg aagaaaagca
     2161 ggaagctatc caggagaagg tcgagaaggc taccgccaag caggccgcaa aagaggccaa
     2221 ggctagtggc aatgacgcca agccggccaa gaagctgagc ctgaagcgct ccggcaagaa
     2281 gaaggaggag aagtaatggc tatcaagcaa tacaacccga ccaccccagg tcgccgtggc
     2341 atgaccagcc aggacttcgc cggtatcact accaagaagc cgctgcgcag cctgacggtc
     2401 attaagcgcc gcggtaacgg ccgcaacaac cagggccgaa tcaccacccg tcaccagggc
     2461 ggcggcgcca agcagttcta ccgaatcgtg aacttcaagc tggacgccgg tacggaagcg
     2521 actattgagc acatcgagta cgacccgaac cgcagcgccc gcatcgcccg tatcaaggac
     2581 aacaagggtg tctatcacta catcctggct gcgcagggta tgaaagtcgg tcagaaactg
     2641 gccagcggtc cggaagcttc catcgaagcc ggcaaccgcc tgccgctcaa gaacatcccg
     2701 gtcggttcga ccatccacgc catcgagctg cagcccggta agggcgccca gatggtccgc
     2761 agcgccggca atagcgccca gctgatgggc cgtgccgaca acggctatgt ccaggttcgt
     2821 ctgccgagcg gcgaagtccg tttggtgaac gagaactgca ctgccagcct cggcgtcatc
     2881 ggcaacgagc agcaccagaa cgtcaagatc ggtaaggccg gtcgttcgcg ccacaagggt
     2941 atccgcccca gcgtccgcgg tgtcgtcatg aacgctgccg accacccgca cggtggtggt
     3001 gacggtggcc gtcatggtgt cggtggcggt aagaaccacg gtaccgcacc gaagacccca
     3061 tggggccaga agacgctcgg ttacaagacc cgccgtcgca agagcaccaa caagttcatc
     3121 gtaagaagcc gtcatgcggc caagaggaaa taaggagtaa ggtatgagtc gttcactcaa
     3181 gaaaggtccg ttcgtcgatt tcaagctcgc aaagaaagtc gccgcgctcg gtactgacga
     3241 tcgcaccatc atcaagacgt gggcccgggc cagcaccatc acgcctgatt tcgtcggccg
     3301 caccatcgcc gtccacaacg gcaagatgca cgtcccggtg ttcatcaccg agaacatggt
     3361 cggtcacaag ctcggcgagt tcagcccgac ccgtaagttc cgctcgcacg gcggtaagtt
     3421 ggcgaagaag taggagttta taagatgcct gtacaagcaa tcgcaaaagg agtcagcctc
     3481 agtccccgca aagtggcggt ggtggccgct ttggtccgtg gccgcagcgt cgatgacgcg
     3541 ctgaccatcc tagagaacac tccgcgccgg agcgccctgg ctgtcagtaa ggtcatcgcc
     3601 agcgctcgcg ccaacgccga ccacaaccac ggctacaagc cggctaccct gcagatcgtc
     3661 gagatcagcg ttaccagcgg cccgcgtctg aagcgcttcc gtccggccgc ccacggccgt
     3721 gccctgcgct tccagcgtcg ttccagtcac atccgtgtcg tcgtcgacgg cgagatgaga
     3781 gaagttaaga aaccggctgc caagccagca accgcaacta agaaggagga gaagtaatgg
     3841 gacagaaggt aaaccccatt agcatgcgcc tccaggtcaa caaggactgg cgcagcaaat
     3901 ggttcgttga caaacgcgac tatgccaagt acctgaagga tgacctggca gtccgccgga
     3961 tgatccagaa caaacttggc tcgcgcgctg caatcaacaa agttgacatc agccgcacgc
     4021 ccaacctcgt gacggtgacc atcgccacgg ccaaggccgg cgtcgtcatc ggccgtggtg
     4081 gtagtggcgc ccaggagctg aaggaaacca tcgagaagat ctacggcaca ccgacccgtg
     4141 tcaacatcga agaagtcaag aagagcgaac tgtacgccaa gctggtcgcc gagaacatcg
     4201 ccaaccagct ggagcgccgc atgagcttcc gccgcgccat caagagcgcc gctgccgcta
     4261 ccatgcgtgc cggtgccaaa ggcatccgta tcgaggtagc cggccgtctg aacggcgccg
     4321 aaatgagccg ccgcgagaag gaaaccgccg gttcggtccc gctgcacacg ctgcgcgccg
     4381 acatcgactt cgcgctcgtc aaagccaaga ccccggccgg tatcatcggc gtgaaggtct
     4441 ggatctgcaa gggggaggca taacatgtta gtccctaagc gaacccttca ccggaaagtc
     4501 cggaaaggca agatccgcgg tgtcgctact agcggtaaca ccatcgcctt cggcagcttc
     4561 gcgctgcagg cccagggcca cgaacgcatc accagccgtc agatcgaggc cagccgtcag
     4621 gcgatgaccc gctatatcaa gcgtggtggt aagatctgga tccgcatttt cccgcacacc
     4681 cccgtgacgc gtaaaccgct ggacgtcaag atgggtagcg gcaaaggcaa cccggaattc
     4741 ttcgtcgcca aggtccgccc cggcaccatc ctgttcgaaa tgcagggtgt tcccgaagag
     4801 accgcccgtg aagccatgcg cctggccgcc cacaagctgc cggtaaagac caagttcctg
     4861 gtccgggagg aggcataaca tcatgaaagt aaccgatatc cgcaaactga gcactgccga
     4921 cctgacgctc gagagcacca agctccgcga ggagatcgcc gaactgaagc gccgcgttat
     4981 cagcggtgag cagcagaacg tgcgcctcat ccgcaacaag cgtaaggacc tggcccgcgt
     5041 gctgacggtc ttaagcgaac agctcgtaaa ggagacaaag taatggctag gacaattgtt
     5101 ggtactgtct ccagcgacaa gggcgacaag accatagtag tgacggtgca gacccgcaag
     5161 acccacccgg tctaccgcaa gcagtacacc gtcagcaaga aattcatggc tcacgacgaa
     5221 cagaacgaag cccaggtggg tgacaaggtc gccatcgtcg aaacccggcc ggtcagtgcc
     5281 cgcaagcact tccgcctgga ccgcatcatc gagaagccgg cgctccgcga aggccagttg
     5341 gtgagcggcg aaactcagga aagtgcggca gtcaaggccg agaaagccgc caagaaggca
     5401 gctgccgagg aggagaccaa gtaatgattc agcaggaaac ccgtttgaac gtctgtgaca
     5461 acagcggtgc caaggaaatc ctctgcatcc gtgtcctcgg cggcacccgc cggcgttacg
     5521 cccgtgtggg tgacgtcatc gtcgccaccg tcaaagacgc caacccgagc ggtaacgtca
     5581 agaagaagtc ggtcgtgaag gccgtcgtcg tccgtaccca ggagaagatc cgccgcaaag
     5641 acggttcgac catccggttc gacgacaacg ccgctgtcat catcgccgac gacaagaacc
     5701 cgcgagcaac ccgtatcttc gggccggtcc cacgcgaact gcgtgacctc ggctacgcca
     5761 agatcatctc gctcgccccg gaggtactct aatatgagca cgaagcaaac caccaagact
     5821 acctacaaga tccgcttgaa gaagggcgac accgtcgttg tgcgctctgg caagtacaag
     5881 ggtcagaccg gtaagattac cgctactcac ccgagcgaga acaaggtgac cgtagaaggt
     5941 atcaatatcg tcaagaagca cgtcaagccg aaccgtgagc acccgcaggg cgccatcgtc
     6001 gaactgacga agcccatttg ggtcagcaag gtcgccatcg tcgagccgac cagcaagaaa
     6061 gccagcaaga tcggctataa cgtcgcagct gacggtacca agacccgtgt atttaagaag
     6121 actggaaagg agatcaagta atggctacag ctaccaaaac agcgaaacct gcgaagcaag
     6181 ctgccagcac agcccgtctc cgccaggatt atcagacccg catcgtgccg gaactggaga
     6241 aagaactagg tctgaagaac gttcatcagc tgccgaagct ggagaagatc gtcgtgaacg
     6301 tgggcctcgg caaggccaag gatgacaaga aagtcatcga ggctgccaag aacacgttga
     6361 ccaagattac cggtcaggca ccggtcgaaa ccttcgccaa gaagtcgatt gcgggcttca
     6421 aactgcgtga aggcaacatg atcggcatca aggtcacctt gcgcggtgac aaaatgtacg
     6481 agttcgccga tcgactgatc aacatcgtgc tgccgcgtct gcgcgacttc cacggtgttt
     6541 ccccgaagtc gttcgataaa gcgggcaact acagcatcgg catgaccgac caatcggtct
     6601 tcccggagct taccttcgac gagaccacca ccgcccatgg cctgcaggcc gtgttcgtca
     6661 tcgacgccca cgagccggct cacgcccgcg ccctattaga gaagttcggt atgccgttcg
     6721 a
//
LOCUS       DASSFD010000026         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1181748, whole genome shotgun sequence.
ACCESSION   DASSFD010000026 DASSFD010000000
VERSION     DASSFD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181748"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFH39_00605"
     CDS             complement(<1..895)
                     /locus_tag="VFH39_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924314.1"
                     /translation="MSEQVADNGRERMGGMEKPEFTLQRITDGFEFGDGVISGCEGDP
                     ETLRIIDDAIETDPAILVAIDAEDDGSPLEDDGCGDGRGVGRIFGGGKKFKRSLNRAK
                     VFGGAAAMAAAARIGLGEAEGRPLNKVFDDSIEELNEKHIDFGAHTADHIKAGREALD
                     SGCGAIDGAPEVVRAAVKFEQAIRGVIKSLHIDDEGLDDVFANYRRYDEQLPALQPYS
                     GKQVMDRIVGAGKVVKELVGGHKERRIVINAVRGYTVDQGLIRARTGDKAQVFAFDSW
                     RLEDIAARLQPEDSRAQHKALL"
     gene            1038..1460
                     /gene="mscL"
                     /locus_tag="VFH39_00610"
     CDS             1038..1460
                     /gene="mscL"
                     /locus_tag="VFH39_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET6924315.1"
                     /translation="MFKEFKKFILRGNVVDLAVAVVIGAAFNSVVQAFVKDIITPIVA
                     IFYGGKESEFAARSFTVHGSTFLYGDFVNTAVSFLLIALVVFFLVVQPINRLISLAKR
                     DDKTEEPTTKKCPACLSEINIKATRCAFCTTKLEDTKS"
     gene            complement(1457..1846)
                     /locus_tag="VFH39_00615"
     CDS             complement(1457..1846)
                     /locus_tag="VFH39_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924316.1"
                     /translation="MVNRSCAELLFNDDELRQVNRTAARRRTVALALGSVLGFAGTVA
                     ALTGLLHASARSLAELSSGSVAPSESPFGHGNDVLLYGGSATVLAGLALAGAADSVRV
                     YSENRQAYGRLRQVAPEDITGMQAPNP"
     gene            complement(1889..>2366)
                     /gene="atpC"
                     /locus_tag="VFH39_00620"
     CDS             complement(1889..>2366)
                     /gene="atpC"
                     /locus_tag="VFH39_00620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET6924317.1"
                     /translation="LLYARRHKRSKGREEAVIHLQLVTLTGMKFDDDVYEVVVPTLDG
                     RIGVMPGHMPLISAATNGVISIRRNAKDPDRDMEHFATHGGVVEVASDRLRIIVDEAD
                     TPDEINEAEAQKAYAAAEKLRAEAKDQVSLEHAQALVDRSSVRLQVAGLKRRHQRN"
BASE COUNT          480 a          743 c          605 g          538 t
ORIGIN      
        1 ttagcagcgc cttatgctgc gcccgcgaat cctccggttg taacctggct gcaatatctt
       61 caagccgcca gctatcaaaa gcgaaaacct gtgctttgtc gccggtacgg gcgcggataa
      121 gcccctgatc gaccgtatag ccgcgcaccg cattgatgac gatacggcgc tccttgtgac
      181 cgccgaccag ctctttcacc acctttccgg cacctacgat gcgatccatg acctgcttgc
      241 cgctataagg ctgcagcgcc ggcagctgct cgtcgtaccg ccgataattg gcaaacacgt
      301 catccaagcc ttcgtcgtcg atgtgcaaag atttgatcac gccgcgtatc gcctgttcga
      361 actttacggc cgcgcgtaca acttccggcg ccccgtcaat agccccgcag ccgctgtcca
      421 atgcttcacg cccggccttt atgtggtcgg cagtgtgcgc gccaaaatct atatgttttt
      481 cgttcaactc ttctatcgag tcatcgaaca ctttatttag cggccggccc tcggcctcgc
      541 ccaagccgat acgggcagcc gcggccatag cggctgcacc gccaaaaacc ttggcgcggt
      601 taaggcttct tttgaacttt ttgccgccgc cgaaaatcct gccgacgccg cggccgtcgc
      661 cgcagccgtc atcttcgagc gggctgccgt cgtcttcggc gtctatggcc accaatatgg
      721 ccggatcggt ttctatggcg tcatctatga tgcgcaatgt ttccgggtcg ccctcgcacc
      781 ccgatataac accgtctccg aattcaaacc cgtccgttat acgctgcagc gtaaactccg
      841 gcttttccat accgcccatc ctttccctgc cattatcggc aacctgttca ctcatttcta
      901 cctctgcatt atcatgtgtt ttgcatcaca aaccgtataa tcctacccct ggggcaatca
      961 ttttgcaagt agaaatattg ctctcccggg ctgcggccga ttatctgcta ttatgatatg
     1021 cagtaacgag gcacgccatg ttcaaagaat tcaaaaagtt tatcctgcgg ggcaatgtcg
     1081 tcgatctggc agtagcggtg gtcatcggtg ccgccttcaa cagcgtcgtg caggctttcg
     1141 tgaaagatat catcacgccc atagtcgcta tattttacgg cggcaaagaa agcgagttcg
     1201 ccgcccgttc gttcaccgtc cacggcagta cgttcctgta cggcgacttc gtcaacaccg
     1261 ccgtgtcgtt cctgctcatt gccctggtag tgttcttcct ggtggtgcaa cccataaacc
     1321 ggctcatttc cttggctaag cgcgacgata agaccgagga gccgaccacc aagaaatgtc
     1381 cggcatgcct tagtgaaatc aacatcaaag ccacccgctg cgcgttctgt actaccaaac
     1441 tggaggacac caagtcttag ggattcggcg cctgcatacc ggtgatatcc tccggcgcca
     1501 cttgccggag ccggccgtac gcctgacggt tctcagagta aacacgaacg ctgtcggcgg
     1561 caccggcgag tgccagaccg gcgagtacgg tcgcactgcc gccataaagc aaaacgtcat
     1621 tgccgtgacc gaacggcgat tcgcttgggg caacgctgcc gctggacagc tctgccaggc
     1681 tgcgggctga agcgtgcagc aaaccggtaa gggcagcaac cgtcccggca aaaccgagca
     1741 cgctgccaag cgccagggca acggtgcgcc ggcgggcagc cgtacggttt acctgacgta
     1801 attcatcatc gttaaagagt aattccgcgc atgagcggtt aaccataccg tcagtatagg
     1861 aaaaacggct gcaaatgcaa gcggacggtc agttgcgctg gtgccgtctt ttgagcccgg
     1921 ccacttgcaa gcgcacggag ctgcggtcga caagggcctg ggcgtgctcg agcgacacct
     1981 gatctttggc ttcagccctc agtttctcgg cggcggcgta tgccttttga gcttccgctt
     2041 cattgatctc atccggtgtg tcggcctcat ccacaatgat acgcaagcga tcgctggcca
     2101 cctccaccac gccgccatga gtagcgaaat gctccatgtc gcgatccgga tccttagcat
     2161 tacggcgaat gcttatgacg ccgttcgtgg ccgcactgat gagcggcata tgccctggca
     2221 tgacgcctat tcgtccgtcc aaagtcggta cgactacctc gtacacgtcg tcgtcgaact
     2281 tcattccggt cagcgtaacc agttgcagat gaatcactgc ttcttctcgt cctttacttc
     2341 gcttatgtcg ccgcgcatat aaaagg
//
LOCUS       DASSFD010000027         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1144945, whole genome shotgun sequence.
ACCESSION   DASSFD010000027 DASSFD010000000
VERSION     DASSFD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144945"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..484)
                     /gene="rpsU"
                     /locus_tag="VFH39_00625"
     CDS             complement(269..484)
                     /gene="rpsU"
                     /locus_tag="VFH39_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET6924318.1"
                     /translation="MIQVTKKDAKESTENLLRRFNRKVQQSGTVAIVKSGQFFEKPTS
                     KRERRAKAIIRSERKAAKLRKIKLGQR"
     gene            604..891
                     /locus_tag="VFH39_00630"
     CDS             604..891
                     /locus_tag="VFH39_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924319.1"
                     /translation="MSKHETQTLLIIGAPVALGLLGGFAYDRFLHLDDPGGLALVVGF
                     VEGFIVMCVAALLRGLVDLTLKRRSSRLAGSTMVYAGITLLVAASTVAITW"
     gene            complement(1018..1308)
                     /locus_tag="VFH39_00635"
     CDS             complement(1018..1308)
                     /locus_tag="VFH39_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6924320.1"
                     /translation="MLDIFITSRVRRKIIVIYAKYPDFKTHVRGLAKLIKEDAGNIQR
                     ELKRLEKVGFLISEKQGNTKVYYTNKQFPIFKELQSIVLKSQRTTQKKTITG"
     gene            1456..>3137
                     /gene="priA"
                     /locus_tag="VFH39_00640"
     CDS             1456..>3137
                     /gene="priA"
                     /locus_tag="VFH39_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6924321.1"
                     /translation="MRYYLVWVRSQKYRGHDPLTYAYDGELAAGTVVLVELQRELVLG
                     FVAGEVAKPSFATKPLRTVFDLPPLPPQVPQLAAWLQAFYATTLGVAAQQLLPPLLSE
                     KTARDYEPVVANTISGKLPPLTDDQTTVVNNIKAADTYVLHGKTGSGKTRVYLDVALT
                     TVTAGRSVLVLTPEISLTSQLARGFQAVFGNQVLILHSQLTPQERRTAWLRILTAEVP
                     VVVIGPRSALFSPFRNLGLVVVDESHEGTYKQEQAPYYHAVRVASQLAHLHGATLVLG
                     SATPSVSDYFLAEQRGKPILKMTALAAYGAPGSSEAERWKVEGGNPESTFQNPASSTS
                     KVDIVDLKDRGQFSRSQLLSAPLVNAVQAALGRGEQSLIYLNRRGTARVVLCENCGWQ
                     AVCPHCDLPLTYHADASQFRCHTCGYHEPLRVSCPVCGHPNVLFKSFGTKALADEVQR
                     LFPEARVQRFDTDNKKDERFERHYEAVRAGEVDILVGTQLLAKGLDLPRLSTLGVVLA
                     DSSLYLPDFSASERTYQLLTQVLGRVGRGHVQGHAVVQTYHPESPILADAIAG"
BASE COUNT          623 a          964 c          901 g          649 t
ORIGIN      
        1 gccttcaggt cagtctccag tctctcttcc aacatctcag atatatccta ccacagcctt
       61 gtgcgaagct gaaccgtttt acctcttgga gcactcaata tacttgctaa tgtcaacaat
      121 gtccataccg aagccaccca caaaaacaga ggactgtttt agaggagtgc tgttttggat
      181 gctcggcgag aaccgaagcg cagcgtacta aaggtacgtg agcatcggat cgcagcagag
      241 cgccagaaat gcgcccgcta aaactgtcct accgctggcc gagcttgatc ttacgcaact
      301 tggccgcctt ccgctcactg cggatgatag ccttagcccg tcgctcgcgc ttgctggtcg
      361 gcttctcgaa gaactgacca ctcttgacga tggccacggt gccggactgc tggaccttgc
      421 ggttgaaacg gcggagtagg ttctccgtcg attccttggc gtctttcttg gtaacttgaa
      481 tcatatcgac gtcctactat agcgcaacag atacgaagat gcaagtaaga ggaatctggg
      541 ttcccgaacc ccaggggccg acggatcgcg tcaatatcga tagcgctgcg ttatactgcc
      601 ttcatgagca agcatgaaac acaaacgctt ttgataatcg gggctcctgt tgccctaggg
      661 ctactaggcg gtttcgccta tgaccggttc ctccatctgg atgacccggg agggttggca
      721 ctggtggtcg gtttcgtaga aggattcatc gtcatgtgcg ttgctgccct gctgcgcggc
      781 cttgtcgacc tcacactgaa acggcgcagc agccggctgg ccggctcgac catggtctac
      841 gccggcatta ccctgctggt agcggcgagc accgtcgcga taacatggta atcccctggc
      901 ttcccactcg ggtatcgatc aacagcgcct aagttgaggg ggcagccctt caaaacagcc
      961 tccctgacat gcgtagcggc cctaggacgg gccgctggaa cgaagcagta tcggacacta
     1021 cccggtaatc gtcttcttct gggtggtgcg ctgactcttc agcacgatgg actgcagctc
     1081 tttgaagatc gggaactgct tgttggtgta gtacaccttg gtgttgccct gtttttcgga
     1141 gatgaggaaa ccgaccttct cgagccgctt cagctcgcgc tggatattgc cggcgtcttc
     1201 tttgatcagc ttggccaggc cgcggacgtg cgtcttgaaa tcaggatatt tggcgtagat
     1261 aacgatgatc ttacgacgga ctctcgatgt aataaaaata tccagcatgg cgcgaacgct
     1321 tccttgacgg ctccctctta cattgttatt gcgacaacga acagtatagc ggtccgcgtg
     1381 cgcacaatca aggctcattt ttgcgccaaa acgtccaatt cgtgataaac cgggccggcg
     1441 gactatactg taaacatgag gtattacctg gtgtgggtac ggagtcagaa gtaccgcggg
     1501 cacgatccgc tcacctacgc atacgacggt gagttggccg ccggtacggt ggtgctggtg
     1561 gagctgcagc gcgagctggt gttgggcttc gtggcgggcg aagtcgccaa gccgtcgttt
     1621 gctaccaagc cgctacgcac cgtcttcgac ctgccgcccc tgccgccgca ggtgccgcag
     1681 ctggccgcct ggttgcaagc gttctacgcc accaccttgg gcgtcgcggc ccagcaattg
     1741 ctgccgcctt tgttgtcaga aaaaacggct cgcgactacg agcctgttgt agcgaatacc
     1801 ataagcggca agttaccgcc gctaacagat gaccaaacga ccgttgtaaa caatataaaa
     1861 gctgccgata cgtacgtact gcatggcaaa accggcagcg gcaagacacg cgtctacctt
     1921 gatgtagcgc ttactacagt aaccgccggc cgatcggtgc tggtgctgac gccggagatc
     1981 agcctgacgt cgcagctggc gcgcggcttc caagctgtct tcggtaatca ggtgctcata
     2041 ttgcattccc aactgacgcc ccaggagcga cgcaccgcct ggttgcgcat cctgacggct
     2101 gaggtgccgg tggttgtcat cggtccccgc tccgccctgt tcagcccctt ccgtaacctc
     2161 gggttggtag tggtcgacga gtcgcacgaa ggtacttaca agcaggaaca ggcgccgtat
     2221 taccatgcgg tgcgcgtcgc ctcgcagctg gctcatctgc acggcgccac cctagtgctc
     2281 ggctcggcca cgccgtcggt cagcgactac ttcctggcgg agcagcgtgg caagcccatc
     2341 ctgaaaatga cagcgcttgc cgcgtacggg gcgccaggca gcagcgaagc tgaaaggtgg
     2401 aaggtggaag gtggaaatcc agaatccact ttccagaatc cagcttcgtc gaccagcaag
     2461 gtcgacatcg tagatcttaa ggatcgcggt cagttcagcc gctcgcaact gctgtcggcg
     2521 ccgctggtga atgccgtcca ggcggcgctg ggccgcggtg agcaatcact catctacctc
     2581 aaccgccgtg gcaccgcccg ggtggtgctg tgcgagaact gcggctggca ggccgtctgc
     2641 ccgcactgcg acctgccgct cacctaccac gccgacgcca gccaattccg ctgccacacc
     2701 tgcggttacc acgagccgct gcgcgtcagc tgcccggtct gcggccaccc gaacgtgctg
     2761 ttcaaatcct tcggtaccaa agccctggcc gacgaagtac aacggctgtt cccggaggca
     2821 cgcgtccagc gcttcgacac cgacaacaag aaagatgagc gcttcgagcg ccactacgag
     2881 gccgtccgcg ccggtgaggt cgacatcctg gtcggcaccc agctgctggc caagggcctt
     2941 gacctgccgc gcctcagtac gctcggcgtc gtgctggcgg attcgtcgct gtatctgccg
     3001 gacttcagcg ccagcgagcg tacctaccag ctgctcaccc aggtgctcgg ccgggtcggc
     3061 cgcggccatg tgcaaggcca cgccgtggtg cagacctacc acccagagag ccccatactc
     3121 gccgacgcta tcgccgg
//
LOCUS       DASSFD010000028         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_160652, whole genome shotgun sequence.
ACCESSION   DASSFD010000028 DASSFD010000000
VERSION     DASSFD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160652"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFH39_00645"
     CDS             complement(<1..198)
                     /locus_tag="VFH39_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924322.1"
                     /translation="MHFTDRTDAGRHLADKLVEYEPYETIIYALPRGGVPVAAVVAER
                     LGKPLELIIARKIGHPFDPEYA"
     gene            complement(261..710)
                     /locus_tag="VFH39_00650"
     CDS             complement(261..710)
                     /locus_tag="VFH39_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924323.1"
                     /translation="MKLRYETSVATIVQLIVMLALNFVNQLVAIITSCVKHDNCVENT
                     VVNLLFVIVLAVWLVSLASLGYAAENRRSKRLATLLILAEALVAIVTLFNIKHFPNIL
                     GLVTSIIDFCLAVWVIILAYRLRQVEEKGVAAPTGQRPRRRVRAKAK"
     gene            955..1215
                     /locus_tag="VFH39_00655"
     CDS             955..1215
                     /locus_tag="VFH39_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924324.1"
                     /translation="MAIALPNSELAGDDRRRRGGDEFWQLVGDLRLSGLGDRVLDAYK
                     REQERRLGAAQYAGQLFSVDARADNGDLTARHTVAGPENFLG"
     gene            1323..1904
                     /locus_tag="VFH39_00660"
     CDS             1323..1904
                     /locus_tag="VFH39_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924325.1"
                     /translation="MPNSESVAAVHRSPVEVYEEFYKRYGAMGTRNQQAAGRLFPLLN
                     GRVIHRDRSHDRSTHLIVHETAGNRVRTSLYRVDGEGRPYRHYNYDAAGFVVAMTDGV
                     TISEWQLMTASGTHPGAVNAIDRPYRAELETLRLDLASPDGLRGRLELNRATGAGTLW
                     QAGQELGWILDETASSAEKTLALQPVIPSQRSA"
     gene            1929..2507
                     /locus_tag="VFH39_00665"
     CDS             1929..2507
                     /locus_tag="VFH39_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6924326.1"
                     /translation="MSDIRAVVFDCFGVLVSEQWLSFKHRYFGHDTEQSQEAADLMQQ
                     ADAGYITHDEFIAKVAELSSQDEATVNRYLDRNTANADLFDYIRNELKPRYKIGMLSN
                     AAADWLDELFEPDQLQLFDAISLSFSSGVAKPEAGAYEAIADKLGLQPGQCVFIDDQE
                     RHCTGATEAGMQSVLYKNLHQLKADLEKVLAK"
BASE COUNT          624 a          785 c          780 g          537 t
ORIGIN      
        1 ggcgtactcc gggtcgaacg ggtggccgat cttgcgggca atgatcagct ccagcggttt
       61 gccaaggcgc tcggccacca cggctgccac cggtacgccg ccgcgcggga gggcgtagat
      121 gatcgtctca tagggctcgt attccaccag cttatccgct aaatgtctgc ccgcatccgt
      181 ccggtccgta aagtgcatat accccccata ccgggcatcc tagcaatact tcggggcggc
      241 caatgtacga aatcagacgc ttacttggcc ttggcgcgga cgcgacggcg cggacgctgg
      301 cctgtaggag cggccacgcc cttctcttca acctggcgca gccgataggc cagaattatg
      361 acccacacgg ccaagcagaa gtcgataatg ctggttacaa gcccaaggat gttgggaaag
      421 tgcttgatat tgaacagggt aacgatggcg accagcgctt cagcgagtat cagcaaagtg
      481 gccaaacgct tactgcgccg gttctcggcg gcgtatccga gcgatgccaa cgacaccagc
      541 cataccgcca gaacgatgac gaatagcaga ttcaccaccg tattttcgac gcagttatcg
      601 tgcttcacgc agcttgtgat gatagccacc agctggttga cgaagttcag cgccagcatc
      661 acgatgagct ggacgatagt cgcgacgctt gtttcataac gcaatttcat acctgtttac
      721 agcgtagcgg aaaaacttgt gaaaacaata agaaattcct aacaccacgg tgaggcgtta
      781 tgctttcgcc gcttccgcag gtcgccagta tattgactgc tacgctaccg taagaaatcc
      841 aacagtggca taagcgctat acatgagact atgatcacta cagaggcggt gcacggttgc
      901 cacaggatca tataatgccg cttcctgcag gcaacgtaaa cagggggaac aaacatggct
      961 atagcattac caaacagcga gttggcaggg gatgaccggc gcaggcgcgg cggcgatgaa
     1021 ttctggcagc tcgtaggcga tttacgccta tcgggcctgg gcgaccgggt cctggacgcc
     1081 tataagcggg aacaggaacg gcgcctggga gcagcccagt acgccggcca gctcttttcg
     1141 gtcgacgccc gagccgataa cggcgacctg acagcacgtc atacggtggc cggccccgag
     1201 aactttctcg gttaacttgc atgtaagcac ctatcagcga gcccggctct aacgccgggc
     1261 ttcttgctgc ttgcaaattg tccggcataa gcgtattaca aatacaaagg ttaaaacgaa
     1321 acatgccaaa ctcggaatcc gtagccgccg ttcaccggtc accggtggaa gtttatgagg
     1381 agttctataa gcggtacggg gcaatgggca cccgtaacca gcaagcggcc ggccggcttt
     1441 tccccttgct caacgggcgg gtgatacacc gcgaccgctc gcacgaccgt tcgacgcatc
     1501 ttatcgtcca cgaaactgcc ggtaacaggg tacggaccag cttataccgg gtggatggcg
     1561 agggccgtcc gtatcgccac tacaactacg atgccgccgg tttcgtggtg gccatgaccg
     1621 atggcgtgac tatttcagag tggcaactca tgactgccag cgggacgcac cccggggcgg
     1681 tcaatgccat cgaccggcca taccgggcgg agctcgaaac gttacgctta gaccttgcgt
     1741 cgccggacgg tctccgcggc cggctggagc tcaatcgcgc cacgggtgcc ggtacgctct
     1801 ggcaggcagg gcaggagctg gggtggatac tcgacgaaac ggcgtcgtcg gccgaaaaga
     1861 cgctcgccct gcaaccggtc ataccgtctc aaagaagtgc ctgacgcgtc cgctgctaca
     1921 ataaggacat gtcagatatt cgtgccgtcg ttttcgattg tttcggggtg ctggtgagcg
     1981 agcagtggct gtcgttcaaa caccgttact tcggccatga cacggaacag tcgcaagagg
     2041 cggccgatct catgcagcag gcagatgcgg gctatattac gcacgatgaa ttcatagcta
     2101 aggtggccga actgtccagc caggacgagg cgaccgtcaa ccgctacctg gaccgcaaca
     2161 ccgccaatgc agacttgttc gactacatcc gcaacgagtt gaagccccgc tacaagatcg
     2221 gtatgctctc caacgcagcc gcggactggc tcgacgaact gttcgagcca gaccagttgc
     2281 aactgttcga cgccatctcg ttgtcgttta gttccggtgt agccaagccg gaagccggcg
     2341 cgtacgaagc cattgccgac aagctggggc tacagcccgg gcagtgcgtg ttcatcgatg
     2401 accaggagcg gcactgcacc ggcgctactg aggctgggat gcagtcagtt ctgtacaaaa
     2461 atctgcatca actgaaagcc gacctcgaga aagtccttgc aaaataacgc taatggtgtt
     2521 aatataactt cattaattct tcagaggtag gacagatatg cgattcttca tcggttttct
     2581 ggtaacactc gggctgatca tcttgatcat cgtcctgctt ctgagcggcc gtggcggtcc
     2641 caagcaatcg caacgccctc tggtgctcgg ggatcacgtc aacgctacct cgacggccga
     2701 catactcata gacggaccgg tgaacg
//
LOCUS       DASSFD010000029         5659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_293151, whole genome shotgun sequence.
ACCESSION   DASSFD010000029 DASSFD010000000
VERSION     DASSFD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293151"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..681)
                     /locus_tag="VFH39_00670"
     CDS             complement(16..681)
                     /locus_tag="VFH39_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924327.1"
                     /translation="MSEPERQLPIHEALPAGQRSTLLQIRDWGEVILQSSLRGLDIQS
                     NPPLERANWDDDRRAVRCELLCSRAPGNPGAIKNWYLLRLYTFTDAGPCLEYNLSPTG
                     ALVQAPHFQAPGIVDLLTMQHESAMLTMLKTSQLLPPEDRLPATSAANPWYMPIVGQL
                     LMENTVLKDMCARVHSVQDEAKRELAFAAVMRYVKMKYQLEDARFMRILLDQYMRPDT
                     AVE"
     gene            complement(723..1568)
                     /locus_tag="VFH39_00675"
     CDS             complement(723..1568)
                     /locus_tag="VFH39_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6924328.1"
                     /translation="MSSEFINHTLPRILLTVAIAIVVQMISRRLTQNVVRRVVSHHHF
                     PNKLDEKKREDTIINILDTLVAIVIWLIAILIVLDDMGVNLAALATGAGLFGIIIGLG
                     AQNTIKDFLGGLFVLIENQYRVGDVVTLAGGTTGTGTSGVVEDISLRSTKLRDMEGNL
                     NIVRNGEASVITNRTINYSSVVIDVLVPYEADIDKVEQVMNEVGIALAGTDEFKSLTV
                     EAIQFLRIDSFTPNGATARAVGKVAPAAQWNTAGAYRRRLLPAFAKAGIHVPYAQVEV
                     RQHHK"
     gene            complement(1614..1964)
                     /locus_tag="VFH39_00680"
     CDS             complement(1614..1964)
                     /locus_tag="VFH39_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924329.1"
                     /translation="MLPWFRLALALLPFLGQLRGRAPKDVDVRKTTFDKWFKDKQGRL
                     AIGQKPNLPIIVWAIAELLAWPFSGRAEHFFAMLAFGALFTWAWLEIRWGINRFRRLL
                     GAAVMIYLLVSIAV"
     gene            complement(1991..2536)
                     /locus_tag="VFH39_00685"
     CDS             complement(1991..2536)
                     /locus_tag="VFH39_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924330.1"
                     /translation="MKHFFLGVVMVASLSLSGLMNGWDAFTSPAHPSIDTGSTTWQTC
                     AHYTNHVYEVCSAYILNAAHADLQPYYKYVHDPNSSVAGFVSYRLGSRYTGGAQRLIT
                     QRVANWPSGVNEVDGPDIRILSVTSSLQHNRATLATEESWTVRSASGAVLYQENHVRH
                     TVTMARVPSYLLHKWVVTDIR"
     gene            2713..4026
                     /locus_tag="VFH39_00690"
     CDS             2713..4026
                     /locus_tag="VFH39_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924331.1"
                     /translation="MYTAALKRLQTMDPYARALYIINTIGLVAGTTYGMYYGIFLYRH
                     TFSLSVLAIDGLMGGLGTWLGYMLGVVAVRRQGYNRCIRAAFVLWALVALGTALIANH
                     ITEWFILIGVLKALPGGIYSAVADTIMLREVKTCARSGFFKLNLAIEFIGSIILPAVV
                     GAVVRGADGYRWAFVAAAVVYASGLLVRWRLPKPTLSLDLRGLLGTFKRPLYIPHATN
                     RTLATGFNQLNAFGLTIIPFLLLKNEFSMGLLTSMTALLAAGVALLSRRSKSQNSLKF
                     AFGAQGVRNVFSLLFVLSWSAPFMMIWQLVGKLATPLHDPVQQSLDIHNDSLIMGRDM
                     KTRALQINVLNNTLAFIGTSVAFTAFIAITRTDAHHQRYILELLTLAYAAWRIVNLAI
                     SAGINAQAQDATAYTPLWQRLAFRISLLTALLHTQRTRLLLAIRP"
     gene            4093..4353
                     /locus_tag="VFH39_00695"
     CDS             4093..4353
                     /locus_tag="VFH39_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924332.1"
                     /translation="MNRRRTYLWLAAASGYTGFGVIVGYLLSNHTSNAALWLGIVLLF
                     LYAVLLGMYISARGDARRGNLQENRGPQASSSGNITDLPPKH"
     gene            4428..>5659
                     /locus_tag="VFH39_00700"
     CDS             4428..>5659
                     /locus_tag="VFH39_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924333.1"
                     /translation="MNNHLETPTSVNELTTDYFQERLAEAMVIDDQNQRYDVAQTDTT
                     FDGDLEDFRQLALSGIQAEQAETLDTLRERDNLPYELISKEYEDRVKELAAAEARDAE
                     LSNLDEAQQRRLREAARRAIELAPLVETAAQQLVSARIRNLATLDHGELQAKITQLQA
                     EIADLAELHSLAGTPWPVPRATSGNDPSMLIEDIIPLGPDTHASPENETLCRLFERHG
                     YTHGASIMLTAYLLGNPGVIFTPEQLGQELYVDDSHFEGMTEEKRLETMRLRIHNLLN
                     SRAKVAGFLQDEGFRLQYGWRYFLNPRTRKNLVPKRRIYRAVPLETGIDPGDTFPDDI
                     VMVAEDGTQYREAPVPMPQPTRSTVSEERRNELMRTDDEPDPLQELPVKQLIAAMWDK
                     GLLPKSVDGFATPKKLKVP"
BASE COUNT         1199 a         1660 c         1580 g         1220 t
ORIGIN      
        1 tttgggcacg ccttgctact ccacggctgt atcgggacgc atgtattggt ccagcaagat
       61 acgcatgaag cgggcgtctt ccagttggta cttcattttt acgtagcgca tcacagctgc
      121 aaaggcgagc tccctcttgg cctcgtcctg taccgagtgt actctcgcac acatatcttt
      181 caggacggtg ttctccatga gaagctgacc gacaattggc atataccaag ggttcgccgc
      241 agatgtcgct ggcaaacggt cttcaggagg taaaagctgg gatgttttga gcatggtgag
      301 catggcgctc tcgtgctgca tggtcagaag gtccactata cccggcgctt ggaagtgcgg
      361 agcctgcacc aaggcaccag tcggtgaaag attatattcc aggcaggggc cggcatcggt
      421 aaaggtatag agtcgcaaca agtaccaatt cttgatagcc cccgggttgc ccggggctcg
      481 ggaacataac aattcgcagc gtactgcccg gcggtcatcg tcccagttgg cacgttccaa
      541 gggtgggtta ctctgtatgt ctaagccccg caggctcgat tgcagaatga cctcgcccca
      601 gtcgcgtatc tgcagcaaag ttgatctctg accggctggc aatgcctcat ggatgggcag
      661 ctgacgctct ggttctgaca taggttcagt ctagacgccg gtgacaagac acgcaaccgt
      721 atttacttgt ggtgctggcg gacttcgacc tgggcatacg gtacgtgtat accggctttg
      781 gcaaaggctg gaagcaggcg gcgtcggtag gcaccggccg tgttccactg ggcggccggc
      841 gcgactttgc ctacggcgcg ggcggtcgca ccgttcggtg tgaagctatc gatgcggagg
      901 aactggatcg cttccacggt caacgatttg aattcgtcag tgccggccaa ggctataccg
      961 acctcgttca tcacctgctc caccttgtcg atgtcggctt cgtagggcac caggacgtcg
     1021 atgaccacgc tggagtagtt gatggtccga ttggtaatga cgctggcctc gccgttgcgg
     1081 acaatgttca gattgccttc catgtcccgc agcttggtgc ttcgcaggct tatgtcctca
     1141 acgacgccgg aggtgccggt gccggtagtg ccgccagcca gcgtcaccac atcaccgacc
     1201 cggtactggt tctctatgag gacaaacaag ccgccgagga agtccttgat ggtattctgc
     1261 gccccgagac cgatgatgat accgaacaga cctgctccgg tagccaacgc agccaaattc
     1321 acgcccatat catccaacac gatcagtatg gcaatcaacc atatgacgat ggccaccagc
     1381 gtatccaata tattgatgat agtatcttcg cgcttctttt cgtccagctt gttcgggaag
     1441 tgatggtgcg ataccacccg gcggacgacg ttctgcgtca ggcggcggga tatcatctgc
     1501 accacgatgg cgatcgccac ggtcagcagg atgcgcggta gcgtatggtt tataaactcg
     1561 ctagacatta ccattatcgt accctaaaca ctaacggtac gccagctgcc ggctcagaca
     1621 gcgatagata caagtagata aatcatgacc gctgcgccga gtaggcgtcg gaagcggttg
     1681 atgccccagc ggatttccaa ccaggcccag gtgaacaagg cgccaaaggc cagcatcgca
     1741 aagaagtgtt ctgcccggcc gctgaagggc cacgccagca gttcggcaat agcccatact
     1801 atgatgggca gattgggctt ctgaccgatg gccaggcggc cttgcttatc tttgaaccat
     1861 ttatcgaacg tcgtcttgcg gacgtcgacg tccttcgggg cacgcccccg cagctgcccc
     1921 aggaagggca gtaatgcgag ggccaggcga aaccacggta gcatgcacct agcttacctt
     1981 attcgagcag ttaacggata tcggtaacga cccacttatg gagcagatag cttggcaccc
     2041 gtgccatggt cacggtatga cggacatggt tttcctggta tagcaccgcc ccgctcgcgc
     2101 tgcgtaccgt ccagctttcc tccgttgcca gtgtcgcacg gttgtgctgt aaactcgatg
     2161 tcaccgaaag tatgcggatg tccggaccgt cgacttcatt gactccggat ggccagttgg
     2221 ctacccgttg cgtgatcagc cgctgggcgc ccccagtgta gcgtgagcca agccggtagc
     2281 tcacgaagcc ggcgacgctg ctgttcggat catggacgta cttataatac ggctgcaggt
     2341 cggcgtgggc agcgttcagt atgtaggcgc tgcacacctc atagacgtgg ttggtgtagt
     2401 gggcacaggt ctgccaggtc gtactgccgg tgtcgatcga cgggtgggca ggactggtaa
     2461 aggcgtccca gccgttcatg agcccgctca aacttaagct ggcaaccatc acgacaccga
     2521 gaaagaaatg tttcataccc atcatggtaa ttttaagcgt aaatgctatg cccttacccc
     2581 tgcagctaag atttttacac cacttgtgtt gtaaattcca ttgacaaaaa cgtaagaata
     2641 atcttccagt ggcggagtcg cccgtgtaca attacctgtc gattagggta attaagagag
     2701 aaaacgagca gaatgtacac cgctgcgctc aagcgcctcc agaccatgga cccctatgca
     2761 cgggccctct atatcatcaa tactatcggc ttggtcgccg gtacgacgta tggcatgtac
     2821 tacggtatat ttctgtaccg ccacaccttt tcgctgtcgg tgctagccat cgacggactg
     2881 atgggtgggc tgggtacgtg gctcgggtac atgctgggcg tcgtcgcagt ccgacgacaa
     2941 ggttataacc gctgcatccg ggcggcattc gttctgtggg ctctggtggc gctcggcacc
     3001 gcgcttattg ccaaccacat caccgagtgg ttcatactca tcggcgtctt gaaggcgttg
     3061 cctggtggta tttattcggc ggtggccgac acgattatgc tgcgcgaggt caagacctgt
     3121 gcccgcagtg gctttttcaa gcttaacctg gccatcgaat tcatcggtag catcattctg
     3181 ccggcggtgg tcggggcggt cgtccgcgga gcggacggtt accgctgggc gttcgtggca
     3241 gcggcggtcg tctatgccag cggcttgctg gtgcgctggc gtttgccgaa gcctacgctg
     3301 tcgctggatc tgcgcggcct gctcggtacc ttcaaacgtc cgctgtacat cccgcatgcc
     3361 accaaccgca cgttggctac cggtttcaac cagctcaatg ccttcggtct tactatcatc
     3421 cccttcctgc tgctcaagaa cgagttcagc atgggcctgc tcactagtat gaccgccctg
     3481 ctggcggcgg gcgtcgcgtt gctgtcacgg cgttcgaaga gccagaatag cctgaagttc
     3541 gcgttcggcg cccaaggtgt ccgcaacgtc ttcagcctgc tgttcgtgct gagctggtcg
     3601 gcgcccttca tgatgatctg gcagctggtc ggcaaactgg ccacgccgct ccatgacccg
     3661 gtgcagcaga gtctggacat ccataacgac agcctgataa tgggtcgcga tatgaagacc
     3721 cgtgcgctgc aaataaacgt cctcaacaac acgctggcgt tcataggcac cagcgtggcg
     3781 tttaccgctt tcatcgccat tacccgtacc gatgcccacc accagcgcta cattctggag
     3841 ctgctgacgc tggcctacgc tgcctggcgc atcgtcaacc tggccatttc ggccggtatc
     3901 aacgcccagg cccaggacgc cacggcatac actccgctct ggcaacggct ggccttccgc
     3961 atcagcctgc tgacggccct gctgcatacc cagcggactc gtctgctgct ggctatccgc
     4021 ccgtagcccg tgcccgcgcg actgtaagga atttctcagc gcgacggtcc gcgaacgtat
     4081 attgtagcta ctatgaaccg acgacgtact tatttatggt tagcggccgc cagcggatac
     4141 accggcttcg gcgtcatcgt tggctatctg ctcagcaatc acactagcaa cgccgcactg
     4201 tggctgggca tcgtcctcct gtttttgtac gccgtgttgc taggtatgta catcagcgcc
     4261 cgcggcgatg cccggcgtgg caatctgcaa gaaaaccgtg gtccccaggc ctcctccagc
     4321 ggcaatatca ccgacttacc acctaagcat taagtgtgac gcccagtttc gcagtttact
     4381 gcacgctagg ttgctaaatg cttactaaga tggtaaaata gcctaccgtg aacaaccatc
     4441 tcgaaacacc tacctctgtc aatgagctga cgaccgacta cttccaggaa cgcctcgccg
     4501 aggctatggt catcgacgac cagaatcagc gctatgatgt cgctcagaca gacaccacgt
     4561 tcgacggtga cctggaggac ttccgccagc ttgcgctcag tggcatccag gcagaacagg
     4621 ccgagacgct cgatacctta cgcgaacgcg ataacttgcc gtatgaactt atctctaaag
     4681 aatacgaaga ccgcgtgaag gaactggcgg ctgccgaagc acgcgacgca gaactgtcca
     4741 atctggacga agctcaacag cggcgcctac gcgaagccgc ccgccgcgcc attgaactgg
     4801 caccactggt cgagacagcg gcgcaacagc tggtcagcgc acgcatccgc aatctcgcta
     4861 ccctggatca cggcgagctg caagccaaga ttacccagct ccaggccgaa atcgccgatc
     4921 tggccgagct gcacagtctg gccggcacgc cgtggcccgt accgcgcgcc accagcggca
     4981 acgaccccag tatgcttata gaagacatca ttccactggg ccctgacacc catgcctcgc
     5041 ccgaaaacga aacgctttgc cgcttattcg agcgacatgg ttatacccac ggggcctcta
     5101 ttatgctcac tgcttattta ctcggaaatc ccggggtcat ctttacgcca gagcagctcg
     5161 gacaagagtt atatgttgat gactcgcatt tcgaaggaat gactgaagaa aagagactgg
     5221 aaaccatgcg tctgcgtatt cacaatctgt tgaacagccg cgcgaaggtt gccggtttcc
     5281 tgcaagatga aggtttccgg ttgcagtatg gttggcgcta tttcctgaat ccgcggacgc
     5341 gtaaaaatct ggttcccaaa cgtcgaatat accgtgccgt accgcttgag acgggcatcg
     5401 acccgggcga cacctttcct gatgatatcg tcatggtggc cgaagacggc acccagtacc
     5461 gtgaagcgcc ggtgcctatg ccgcagccga ctcgcagtac ggtgagtgag gagcgacgga
     5521 acgaactcat gcgtacggac gatgagcccg acccgttgca ggaactgcca gtcaagcagc
     5581 tgattgccgc catgtgggac aaaggtttgc tgccaaaatc ggtcgacggc ttcgctaccc
     5641 caaaaaagtt aaaagtacc
//
LOCUS       DASSFD010000030         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1657234, whole genome shotgun sequence.
ACCESSION   DASSFD010000030 DASSFD010000000
VERSION     DASSFD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657234"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VFH39_00705"
     CDS             complement(<1..1149)
                     /locus_tag="VFH39_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET6924334.1"
                     /translation="MARKPKVDPVAIAQAQRAREEQEVQAAFQKGITALRDFIAPSSI
                     EFTSSYFRLGTRFARTYYVYGYPRQIYTGWLSGMVNLDEVMDMSMYIYPVESQVVLEN
                     LRKKVTQLEAGIQIDAEKGRIRDPGKQAAIMDAEEMRDKLQVGEERFFRFGLYFTIYA
                     DSQDELEFVSHKVESMLGQQLVYSKPATSQQEQGMNSTVPQLVDQLQIRRNFSTGALS
                     TSFPFTSADLTQDNGILYGINMHNSGLVIFDRFSLENGNSVVFAKSGAGKSFTVKLEA
                     LRSMMFGTEIFIIDPENEYQRMCDAVAGAYVRLSLSSPTRINPFDLPRVVDTEEADNA
                     LRSNLITLHGLLRLMMGGAQAQMQAQGGSLLAPALSPSEEADLDAALIE"
     gene            complement(1149..2117)
                     /locus_tag="VFH39_00710"
     CDS             complement(1149..2117)
                     /locus_tag="VFH39_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924335.1"
                     /translation="MNPSLPNQPSQPAAPGGQSYAPGAMPQPQLQPLGVAPPAPGATP
                     TYGATANVPPATYAAAPQPVAVGQQPGQPAVAPAPKTNPNSTQNTLQIAEIRDGIVIM
                     SDGSYRSVIMVKSINFDLMSVQEQEAVEYAYQGFLNSLYFPVQIFIRSQRIDLQPYIE
                     KLDKIRTEHDNMLLALLMEDYIMYIDQLSQQTNIMDKKFYVVVPYFPIVDVQKALTQS
                     KNFFSGLSALFNSKEQHVTINEAELEKAKTELRNRVQAALQGLLQCGIQGIPLDTQEL
                     IELYYDTYNPDTATRQQLKNIDDLTADVITKGQGTAVQAHLQRELQ"
     gene            complement(2222..3286)
                     /locus_tag="VFH39_00715"
     CDS             complement(2222..3286)
                     /locus_tag="VFH39_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HET6924336.1"
                     /translation="MSTYKVIQDIEAEDHILGPLSLRQFIYGLIALFCFYLCYLFIAK
                     HVAFLCALFLPQGLFTAFFAIPFGQDQPTELWALAKIRFFLKPRRRIWDQSGVKELVT
                     VTAPKKVEERRTNGLSQTEVQSRLNALANTIDSRGWAIKNVNVNLNAVGAGPAMDNSD
                     RLVDMSSIPQPVPDYDVQASDDILDEQNNPIAQQFQQMINSSEQAHRQKLQDMMSGNA
                     PVSPQAVPASPTDNGVTPDYWFMQAPVAAPVPTQLVAPATPVANLPDPTPEEQAIAAR
                     AKQAHEQPDLSLGHLKTIQPIDHNKPVAPPAATTPPAPNPAAFLAHDNDKSIDVIARE
                     AKRINHQDENGDGEVVISLH"
BASE COUNT          603 a          916 c         1066 g          786 t
ORIGIN      
        1 ttcgataagg gcggcgtcga ggtcggcttc ttcgcttggg ctcagtgccg gtgccagcag
       61 cgagccgcct tgggcctgca tctgcgcctg ggcgccgccc atcatcagcc gcagcagacc
      121 gtgcagcgta atgaggttgg agcgcagcgc attgtcggcc tcctcggtgt cgacgacgcg
      181 tggcaggtcg aacgggttga tacgcgtcgg gctgctcaaa ctgaggcgca cgtaagcccc
      241 ggcgacggcg tcacacatcc gttggtactc gttctcgggg tcgatgatga atatctcggt
      301 gccgaacatc atgctgcgca gggcttcgag cttgacggtg aacgacttgc ccgcaccgga
      361 cttggcgaag accaccgagt tgccgttctc gagactgaag cggtcaaaga tgaccagacc
      421 ggagttatgc atattgatgc catacaggat gccgttatcc tgcgtcaggt cggcgctggt
      481 gaacgggaag ctggtgctga gggcgccggt agagaagttg cgccggattt ggagctggtc
      541 gaccagctgc gggacggtac tgttcatccc ctgctcctgc tggctggtgg ccggcttgct
      601 gtagaccagc tgctggccga gcatactctc gaccttgtgg ctgacgaact ccagctcgtc
      661 ctgactgtcg gcgtagatgg tgaagtacag gccgaagcgg aagaagcgct cttcgccgac
      721 ctgcagcttg tcgcgcattt cttcggcgtc catgatggcg gcctgcttgc cgggatcgcg
      781 gatgcgtccc ttttcggcgt cgatctgaat gccggcctcc agctgtgtca ctttcttgcg
      841 caggttttcg agcactacct gactttcgac cgggtagatg tacatgctca tgtccatgac
      901 ttcatcgagg ttgaccatgc cgctcaacca gccggtgtag atctgtcgcg ggtagccata
      961 gacgtaatag gtgcgggcga aacgggtgcc gagccggaag taggaactgg tgaactcgat
     1021 ggaactcggc gcgatgaagt cacggagcgc ggtgatgccc ttctggaatg ctgcttggac
     1081 ctcctgctct tcgcgggcgc gctgcgcctg agcgatagcg accgggtcga ccttgggctt
     1141 gcgggccatt actggagctc cctctgcagg tgggcctgca cggcggtgcc ttgccccttg
     1201 gttatgacgt cggcggtcag gtcgtcgatg ttcttcagtt gctgtcgggt agcggtatcc
     1261 ggattgtagg tgtcgtagta cagctcgatc agctcttggg tatcaagcgg gatgccttgg
     1321 atgccacact gcagcaaccc ctggagggct gcctggacgc ggttacgcag ctcggttttg
     1381 gctttttcca gctcagcttc attgatggtg acgtgctgct ccttgctgtt aaacagtgcc
     1441 gatagtccgc tgaagaagtt cttgctctgg gtgagcgcct tctgcacatc cactatgggg
     1501 aaatacggca ccactacgta aaacttcttg tccatgatgt tggtctgttg gctgagctgg
     1561 tcgatgtaca tgatgtaatc ttccatcagc agcgccagca gcatgttgtc atgttcggtg
     1621 cggatcttgt cgagcttttc gatgtagggc tgcaaatcga tgcgctgcga acggatgaag
     1681 atctgcaccg ggaagtacag cgagttcaaa aacccttggt aggcgtactc gaccgcttcc
     1741 tgttcctgaa cgctcatcag gtcgaagttg atactcttga ccatgatgac gctgcggtag
     1801 ctgccatcgc tcataatgac gatgccgtcg cggatttcag cgatctgcag cgtgttctgc
     1861 gtactgttcg ggttggtctt cggtgccggg gcgactgccg gttggcccgg ttgctggccg
     1921 acggcgaccg gctgcggagc ggcagcatat gtggcgggtg gcacgttggc agttgccccg
     1981 taagttgggg tagcaccggg cgcgggtggc gcgacaccga gcggctgcaa ctggggctgc
     2041 ggcatggcgc cgggcgcgta tgactgacca ccgggcgctg ccggctgact aggctgattc
     2101 ggtaaagatg gattcatgaa aacgtttatt tcccaccccg agactcgggc agacatgtga
     2161 ccaaggccac tatacacacc ttattttact ctaataaagc aagtgaaaac aggccaaaat
     2221 gctaatgcag actgatgacg acttcgccgt cgccattctc gtcctggtga ttgattcttt
     2281 tggcctcgcg ggcgatgaca tcgatgctct tgtcgttatc atgggccagg aaggccgccg
     2341 ggttcggggc gggaggcgtt gtggctgccg gcggggctac cggtttgttg tggtcaatcg
     2401 gctggatggt cttgaggtgt ccaaggctga ggtcaggctg ctcgtgggcc tgtttggcgc
     2461 gggcagcaat ggcctgctct tccggcgtgg ggtccggcag gttggccacc ggggtcgccg
     2521 gggccaccaa ctgcgtcggt accggcgcgg cgaccggcgc ctgcataaac cagtaatcgg
     2581 gcgtgacgcc attatcagta gggctagccg gtaccgcttg tggactgacc ggcgcgttgc
     2641 cgctcatcat gtcctgcagt ttctggcggt gtgcctgttc ggaactgttg atcatctgct
     2701 ggaactgctg ggcgatcggg ttattctgct cgtccagaat gtcatcagat gcctgtacat
     2761 cgtaatccgg caccggttgg gggatgctcg acatgtccac caggcggtcg gaattatcca
     2821 tggccggacc agctccgacg gcattcaggt tgacgttgac attcttgatg gcccagccgc
     2881 ggctgtcgat ggtattggcc agagcattca aacggctctg cacttctgtt tgcgacagcc
     2941 cgttcgtccg tcgctcctcc accttcttcg gtgcggttac tgtcaccagc tccttgacgc
     3001 cgctctggtc ccagatacgc cgccgcggtt taagaaagaa gcgtatcttc gccagcgccc
     3061 acaattcggt cggttggtcc tggccgaacg gaatggcaaa gaatgcagta aacagcccct
     3121 gtggcaaaaa cagcgcgcac aagaaagcca cgtgttttgc gatgaacaga taacagaggt
     3181 agaagcagaa caatgcaatc agcccatata tgaactgccg caggctcaag ggccccagta
     3241 tgtggtcttc ggcttcgata tcttggatga ctttatatgt ggacatacaa ctctaatttt
     3301 aaccctgcat gtccttaact ttgaggattc tggctgttat atgggtctcc tggtcgggta
     3361 gggccggcag c
//
LOCUS       DASSFD010000031         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1330678, whole genome shotgun sequence.
ACCESSION   DASSFD010000031 DASSFD010000000
VERSION     DASSFD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330678"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFH39_00720"
     CDS             complement(<1..666)
                     /locus_tag="VFH39_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6924337.1"
                     /translation="MEYLRRFTSQVRTRLLLVLLFDNAVFLLDWFIVEKVLHLDGLVL
                     VITLAVVSALTLTLLPWLSTRYLTQPTKLISEAILHLAPTAAEQVVPSPDPAHARFGR
                     ELVTTLIHQVYELATVADRLKAESTQQAQASANFVADSLPLPLLLFDNAQVLRYANST
                     AATYLGLPANELVGKDLYSLLDMLFTTEETYDKWLQYVRANNATASQYWERVRLNPAD
                     TRPL"
     gene            complement(727..>2069)
                     /locus_tag="VFH39_00725"
     CDS             complement(727..>2069)
                     /locus_tag="VFH39_00725"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HET6924338.1"
                     /translation="PQRRREPVTAPEPKINYDQNNGATAAPVDDMAVTGDSTNEPVRA
                     SAWTGPATVTEPMSRDPQHTGSERASQPGGKKRWSGLHWPPSKKELLIGIPVLVILLA
                     AGGAALWIKTHTAVPVPPQPKVVHKTVIKKPVVITSKLTGLPVPDDSVNQRPVTGVMI
                     ENSPDARPQSGLDQAGVVFEAIAEGGITRFLALYQDSAPDYLGPVRSVRPYYLQWCMG
                     FDCSLAHVGGSPEGLQDVKAWHAKDLDQFFNAGAYHRISSRYAPHNMYTSMDQLNQVE
                     KNKGYGASNYTGFPRKADQPINAPPSAKTPKINEPVRTAASKIDLNISGYYYNVHYDY
                     DATTNSYKRFEGGAPHVALHKDGSKVQITPKVVVAMVMQYGLEADDHHSQYNVVGSGK
                     AYVFQDGTVTTGTWAKADTTSPLTFADSNGKPIAFDAGQTWLTAVSGDNQVVYK"
BASE COUNT          343 a          592 c          678 g          456 t
ORIGIN      
        1 cagcggccgg gtgtcggcgg ggttgagccg tacccgctcc cagtactggc tggcagtggc
       61 gttgttggcg cggacgtact gcagccactt gtcgtaggtc tcctcggtcg tgaagagcat
      121 gtcgagcaaa ctgtacaggt ctttaccgac gagctcatta gccggcaagc cgagataggt
      181 ggcagccgta ctgttggcat agcgcagcac ctgggcattg tcgaacagca gcagcggcaa
      241 cggcaggctg tcggccacga agttggcact ggcctgggct tgctgcgtcg attcggcctt
      301 cagccggtcg gccacggtgg ccagctcgta cacctggtgg atgagcgtcg tgaccagctc
      361 acggccgaag cgggcgtggg ccggatcggg gctcggaact acctgttcgg cagcggtcgg
      421 ggccaagtgc agaatggctt cgctgatgag tttggtcggc tgcgtcaggt agcgggtgct
      481 cagccacggc agcagcgtga gcgtcagcgc cgagacgacc gccagcgtta tgacgagcac
      541 caagccgtcg aggtgcagta ctttttcgac gatgaaccag tcaagcaaga aaacggcgtt
      601 gtcgaacagc agtaccagca acaagcgggt acggacttgg ctggtgaatc ggcgcaggta
      661 ttccataggt catccggtat cgccgtctag cgctgctggt aagagcccac tccttgccgg
      721 ctcatcttat ttgtatacga cctgattgtc gccgctgacc gcggtcagcc aggtttggcc
      781 ggcatcgaag gcgatcggtt tgccgttgct gtcggcgaac gtcagcgggc tggtggtgtc
      841 ggccttggcc caggtgccgg tggtgacggt gccgtcctgg aagacgtacg ccttgccact
      901 tcccacgacg ttgtactggc tgtggtggtc atcggcctcg aggccgtact gcatcaccat
      961 cgcaacgacg accttaggcg tgatctgtac cttgctgccg tctttatgca gagcaacgtg
     1021 cggtgcccca ccctcgaagc gcttgtagct gttggtggta gcgtcgtaat cgtagtgcac
     1081 gttgtagtaa tagccggaga tgttcaaatc aatcttgctg gcagccgtgc ggacaggttc
     1141 attgatcttg ggtgttttgg cgctgggcgg cgcattgatc ggctggtcgg ctttgcgcgg
     1201 gaagccggtg taattgctgg ccccgtaccc cttgttcttc tcgacctggt tcaattggtc
     1261 catgctggtg tacatattat gtggtgcgta ccgactggag atacggtggt aggcacccgc
     1321 gttgaagaac tggtcgaggt ctttggcgtg ccaagccttc acgtcttgca aaccttccgg
     1381 actgccaccg acgtgcgcca ggctgcagtc gaagcccata caccactgca agtagtaggg
     1441 tcggacgctg cgtacgggcc ccagataatc gggtgccgaa tcctgataca gcgccaggaa
     1501 gcgggtgatg ccgccctcgg cgatggcctc gaagaccaca ccggcctgat ccaagccgct
     1561 ttgcggccgg gcgtccgggc tgttttctat catgacaccg gtgaccggtc gttggttgac
     1621 gctgtcatcc ggtaccggca ggccggtgag tttggaagtg atgacgaccg gcttcttgat
     1681 gaccgtctta tgcacgacct tgggctgcgg cggcaccggc acggcggtat gcgtcttgat
     1741 ccataaagcc gctccgccgg ctgccagcag tataaccaga acgggaatgc caataagcag
     1801 ttccttcttg ctgggcggcc agtgcaagcc ggaccaccgc ttcttccctc ctggttgcga
     1861 ggctcgctcg ctgccggtgt gttgggggtc gcgcgacatc ggctcggtca cagtggccgg
     1921 tccggtccag gcgctggccc tgaccggttc gttcgtactg tcgccggtca cggccatatc
     1981 atctaccggt gccgcggtgg caccattatt ttggtcgtaa tttatttttg gctcgggcgc
     2041 ggtcaccggt tcccgccgcc gctgtggtt
//
LOCUS       DASSFD010000032         7036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1781796, whole genome shotgun sequence.
ACCESSION   DASSFD010000032 DASSFD010000000
VERSION     DASSFD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781796"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFH39_00730"
     CDS             <1..571
                     /locus_tag="VFH39_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET6924339.1"
                     /translation="KPAPQPAKPADKVVTVQSGDNLSKIAQANDTTYVRLFDANDFIS
                     DPNVIHPGDQVKVPAADEQLPDRALPTPAPAPQPAPAPAPAASAPAPAETTESAPVAT
                     VAPAKVSAPAVTGNAAKAYIYSHESGNNPAAVSPNGCFGLGQDCNGVVRAKCGTDYAC
                     QDAYFTNYAISRYGSWDAAAAFWQNNGWW"
     gene            684..1868
                     /locus_tag="VFH39_00735"
     CDS             684..1868
                     /locus_tag="VFH39_00735"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET6924340.1"
                     /translation="MDTRPLAERMRPESLAQVVGQDQLTGTGRIIREIIERKAPTSLI
                     LWGPPGSGKTTLARIIARETGAEFVELSAVTAGKTDVVKVIERAEQNRRLGMRTILFV
                     DEIHRFNKAQQDAFLPHVEDGTIVLIGATTENPSFEVINPLLSRSRVLVLEPLDKPSI
                     TTIVKGAAKELKLNAKRLPAKSVDLLAELSGGDARVALGNLELAVQLADGKTITPEVI
                     ETAAQSRLPGYDKKGENHYNVISACIKSMRGGDANATLYYLARMLQAGEDPKFIARRM
                     VVFASEDIGLEAPAALNLAVSTFLAVERIGLPEAQYNLFHCATVLAKAKKSRAVTDAM
                     HGALAAAKQYPDLPVPLALRNAPTKLMKDLGYNQGYRWEAGFKSKEGFLPPELRDLKL
                     FD"
     gene            1915..2763
                     /locus_tag="VFH39_00740"
     CDS             1915..2763
                     /locus_tag="VFH39_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924341.1"
                     /translation="MKFIQTDNQIEIRMAGTRQALVAAIVLSIGVALAVFGLHLPNNA
                     TTRTTVSSNGTTTTVGPHAGLIMLLFGIGFGFVGLWMLLAARSRRTIIVKNGETTLTV
                     RRLVGGQPESKSFATSTIKAVRLNSYMSGTSGAGTRESTILLVLNDNTLVDIADKGSG
                     GFTMNGINLAGLFRRPPLTDEANRLSQFLGMPLDASDMSSVTGALNTVRDVWLSKGDP
                     QAKLQRIEQTFTQGLPPAEATSAVSPQPNETPPSAGQPTAAAPSGTVAADRSSDGTTG
                     PLPPAS"
     gene            complement(2777..3127)
                     /locus_tag="VFH39_00745"
     CDS             complement(2777..3127)
                     /locus_tag="VFH39_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET6924342.1"
                     /translation="MADYHIKRIYDPAAADDGYRILVDRLWPRGVSKPEAQLDLWLKE
                     VAPSPELRQWFGHDPARFAEFSARYREELAANPAVAELRQLAEKQSRITLLYGARDPQ
                     VNHAAVLRDFLLGQ"
     gene            complement(3158..3892)
                     /locus_tag="VFH39_00750"
     CDS             complement(3158..3892)
                     /locus_tag="VFH39_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6924343.1"
                     /translation="MSPARVLATARRVLMQLRHDPRTIALILVVPCLLITLLKYVFDG
                     QPFVFDRVAPMILGIFPLLMMFLITSIATLRERKLGTLDRLMTMPISKLDFIFGYALA
                     FALLALVQAVVVCAVTLGLLGVTVLGGTWPVLVAAVLAALLGMSLGLFVSAFATSEFQ
                     TVELVMPILMPQILLCGLFVARDQMARLLQWLADVFPLTYSVDAMKQLTLHAAWTGQL
                     TRDLLIVLGCTILALVLGSLTIRRQL"
     gene            complement(3889..4614)
                     /locus_tag="VFH39_00755"
     CDS             complement(3889..4614)
                     /locus_tag="VFH39_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6924344.1"
                     /translation="MTPVVDVRHLTVVLGGRFTALQTVDLQIKPGVITGFIGPSGAGK
                     TTLIRTIVGRQRLTSGSVRVFGKPAGSAGLRSRISYMTQEQAVYGDLTVRENLQYFAT
                     MVGLRRGHARHMVGDVLDTVHLTDKGGVLVRDLSGGQRQRVSLAVALLGNPELLVLDE
                     PSVGLDPVLREQLWEVFHELTQNGRTVIISSHVMDEAERCDDLVLIRDGEVVAHDTPA
                     ALRSKTDSPSVEAAFLKLAGGTP"
     gene            4697..5605
                     /locus_tag="VFH39_00760"
     CDS             4697..5605
                     /locus_tag="VFH39_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6924345.1"
                     /translation="MTADASIVTNQLSKHYAGTKVYALKDLSISVGAGEVYGFLGPNG
                     AGKSTTIRLLMNFIQPTSGSGRILGKDIVRESVAIKRSVGYLSSDMAMYPKLTTEQYL
                     DYMASLQPATDDGYRHKLMRQLHVEPGKRLGDLSRGNRQKVAIVQAFMHKPSVLILDE
                     PSSGLDPLMQEAFYELLRESRARGAAVFMSSHIMSEVQKVCDRVGIIRDGQLLVERNI
                     NEMEQEAAHTLEIRFANKPPIRELERLAGVQLTAHNEHEVTVHLQGKLGPLLSVLSRH
                     DVTQLEVRQLDLEELFMHFYNGGKHS"
     gene            5602..6396
                     /locus_tag="VFH39_00765"
     CDS             5602..6396
                     /locus_tag="VFH39_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6924346.1"
                     /translation="MMWSIIRWTMRQRRWSIFWWSVGMIGFVVLTLIFYPSFKDQANQ
                     LQKSLGNIPPSAMQLFGGTDFFSPVGYLNSQIIYLMLPLLLTILAIGLGSSLIGREEH
                     DTTIESLLARPISRSRLMLAKASAGALITLFVAAVGTATILVMVKAVGMAVPLPNVLL
                     ACAICYLLVLTFGAVAFLLAATGKGRGAALGIATIYAIAGYIIGSLAGTVHWLKVPSV
                     VFPYYYFHSSDILRGTFTWGPVVFYVCLTAGCVILSLVAFRRRDIG"
     gene            complement(6446..>7036)
                     /locus_tag="VFH39_00770"
     CDS             complement(6446..>7036)
                     /locus_tag="VFH39_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HET6924347.1"
                     /translation="VEKVVVAVAHDELTSADQVRADVIESVIAPVLKKYEFDLPAEAN
                     IIVNGTGLWHIPGPESDAGLTGRKIVVDTYGGYARVGGGAFSGKDPSKVDRSGAYAAR
                     FIAKNIVAKGLATKCEVGLAYVIGQPLPLMQTIDTFGTATVSEAELQAFKDALIDTSV
                     KGIIERLDLARPIYRQTSAYGHFGKSGLPWEAGFAA"
BASE COUNT         1483 a         2182 c         2050 g         1321 t
ORIGIN      
        1 caagccggcg ccccaaccgg cgaagcccgc cgacaaggta gtcacagtcc agagcggcga
       61 caacctgagt aagatcgctc aggccaacga caccacctat gtccggctgt tcgatgccaa
      121 cgatttcatc agtgacccga atgtcatcca ccccggtgac caggtcaagg tcccggccgc
      181 cgacgagcag ctgcccgacc gggccctgcc gaccccggca ccggcaccgc aacccgctcc
      241 cgccccggct ccagccgcca gcgcccccgc tccggctgag acaaccgaaa gcgccccggt
      301 agcaaccgtt gctccggcca aagtcagcgc acccgctgta accggtaatg ccgccaaggc
      361 ttacatttac agccacgaga gcggtaacaa tccggccgcc gtcagcccca atggctgttt
      421 cggcctcggt caggactgta acggtgtggt acgtgccaag tgcggtaccg actacgcctg
      481 ccaagacgcc tactttacca attacgcgat cagccgctac ggtagctggg acgcagccgc
      541 cgccttttgg cagaacaacg gttggtggta gagtagtcaa aagtcaaaaa acgcagtaac
      601 ggcatccccc tgccgtacgg cccctgtact tcctggtgca ggggctttct gctgcctaaa
      661 aggaagctat acttatatag aatatggata ccagacccct tgctgaacgg atgcgcccgg
      721 agagcctcgc ccaagtcgtg ggccaggacc agctgaccgg taccggcagg atcatccgcg
      781 agattataga acgcaaagcc cccaccagcc tcatcctgtg ggggccgccc ggcagcggca
      841 agacgaccct ggcgcgcatc attgcccgcg aaaccggagc ggaattcgtg gaactcagtg
      901 cggtcacggc cggaaaaacc gacgtcgtaa aagtaattga gcgcgccgaa cagaaccgcc
      961 ggcttggtat gcgcaccata ctgttcgtgg atgagatcca ccgcttcaac aaggcccagc
     1021 aggatgcgtt cctgccccat gtagaagacg gcaccatcgt cctcatcggc gccacgaccg
     1081 agaacccgag cttcgaagtc atcaatccct tgctgagccg ttcgcgggtg ctcgtgctgg
     1141 aaccacttga caagccatct attaccacca ttgtcaaggg ggctgccaag gagctcaaac
     1201 tgaacgccaa acggctgcct gccaaaagcg tcgacctgct ggcggaactg tcgggcggcg
     1261 atgcgcgcgt ggctctcggc aacctggaac tggccgtcca gttggcggac ggcaagacca
     1321 tcaccccgga agtcatagaa acggcagccc aaagccgcct gccgggttac gacaaaaagg
     1381 gcgagaacca ctacaacgtc atcagcgcct gcataaagag catgcgcggc ggggacgcca
     1441 acgccacctt gtattacttg gcccggatgc tgcaagccgg cgaagacccc aagttcatcg
     1501 cccgccgcat ggtcgtgttt gccagcgagg acatcggcct ggaagcgccg gcagcgctga
     1561 acctggccgt gtcgactttt ttggcggtcg agcgcatcgg tctgccagaa gcccagtaca
     1621 atttgttcca ctgcgccacc gtcctggcga aagccaagaa atcgcgcgcc gtcaccgatg
     1681 ccatgcatgg tgccctggcc gccgccaaac agtaccctga cctgccggtg ccgctggcac
     1741 tccgtaacgc ccccaccaag ctcatgaaag acctgggcta taaccaaggc taccgctggg
     1801 aggccggctt caagtccaag gaaggtttcc tgcccccgga gttgcgcgac ctgaaactgt
     1861 tcgattgacg tatcggcttg tgcttatgca acgcgtaccg gtactatagg gcttatgaaa
     1921 ttcatacaaa cagacaatca aatagaaatc aggatggccg gtacaaggca ggcactggtc
     1981 gctgccatcg ttttgtcgat aggtgtcgcg ctggcggtat tcggcctgca tctaccgaac
     2041 aacgcgacga cgcgcactac cgtcagttcc aatggaacga cgaccacggt cggcccgcac
     2101 gccggcttga tcatgctgct cttcggcatc ggcttcggct tcgtcggctt gtggatgctg
     2161 ctggcagcgc gcagccgccg taccatcata gtcaaaaacg gcgagacgac cctgacggtc
     2221 cgccgtctgg tcggcggaca gcccgaatcg aagagcttcg cgacgagcac aatcaaagcg
     2281 gttcgcttga actcctacat gagtggtacg agtggtgcgg gtacccgcga aagcaccatt
     2341 ttgctcgtac tgaacgacaa tacgctggtc gatatcgccg ataaaggcag cggcggcttt
     2401 accatgaacg gtataaacct ggccggctta ttccgccgtc caccgctcac cgatgaggcc
     2461 aaccgcttaa gccagtttct ggggatgcca cttgacgctt ccgatatgag cagcgtgacg
     2521 ggtgcgctca acacggtgcg cgacgtctgg ctcagcaaag gcgatcccca ggcaaaactc
     2581 cagcgcatcg aacagacttt cacccaggga ttgccaccgg ccgaagcaac gtccgccgtc
     2641 agtccgcagc ccaacgagac accgccgtct gccggacagc cgacggccgc cgcgccaagc
     2701 ggtaccgtag cagccgaccg ctcatcagat ggtacaaccg gcccgttacc gcccgcttca
     2761 taagccggac attctcttat tggccgagca agaagtcacg cagcacggcg gcgtggttta
     2821 cctgcgggtc gcgggcaccg tacagcagcg tgatgcgcga ctgtttttcc gccagctggc
     2881 gcagttctgc cactgcggga ttggccgcca attcttcacg gtaacgggca gagaactcgg
     2941 cgaagcgggc cggatcgtgg ccgaaccatt ggcgcagttc gggtgagggc gccacttctt
     3001 taagccagag gtcgagctgg gcctcgggct tactcacccc acggggccat aagcggtcca
     3061 ccaggatccg gtagccgtca tctgccgccg ccgggtcata gatacgcttg atgtgataat
     3121 ccgccatgct tctatgatac tacgtgcctg tagcgggtta tagctggcgg cggatggtga
     3181 gcgagccaag gaccagcgcc agaatggtac agccgagcac gatgagcagg tcccgggtga
     3241 gttggccggt ccaggcggca tgcagggtca gctgtttcat ggcgtcgaca ctatacgtga
     3301 gtggaaagac atcggccagc cattgcaaca atcgggccat ctggtcgcga gcgacgaaca
     3361 gcccgcagag cagaatctgc ggcatgagga taggcataac cagctcgacg gtctggaatt
     3421 cgctggtagc gaaggcactc acgaacaagc cgagcgacat gccgagcaag gccgccaaca
     3481 cggccgctac cagtaccggc caggtaccgc cgagtaccgt cacgccgagc aggccgaggg
     3541 tcaccgcaca gaccaccacc gcttgcacca gcgccagcag ggcgaacgcc agcgcatagc
     3601 cgaaaatgaa gtccaatttg ctgatgggca tggtcataag acggtcgagc gtgccgagct
     3661 tgcgttcgcg taaggtggcg atagaggtta taaggaacat cataagcagc gggaagatgc
     3721 cgagaatcat cggggcgaca cggtcgaaga cgaacggttg gccgtcgaag acgtatttga
     3781 gcagcgtgat aagcaggcac ggcacgacca ggatgagtgc gattgtccgc gggtcgtgac
     3841 gcagttgcat gagtacccgc cgggcggtgg ccagcacacg ggccggactc atggggtgcc
     3901 tcccgccagt ttgaggaatg ccgcttcgac cgaggggcta tcggttttcg accgcaaagc
     3961 ggccggcgta tcgtgcgcca ccacctcacc gtcgcggatc agtaccagat catcgcagcg
     4021 ttcagcttcg tccatgacat ggctggatat gatgaccgtc ctgccgtttt gtgtcagctc
     4081 gtggaatacc tcccatagct gttcgcgcag caccgggtca agccctacgg acggttcatc
     4141 cagtaccaat agttccgggt taccgagcaa agccacggcc agtgaaacgc gctggcgctg
     4201 accgccggac aagtcgcgga ccagcacacc gcctttatcg gtcaggtgta cggtatcgag
     4261 gacgtcaccg accatgtgtc gtgcatgtcc tcgccgtaat ccgaccatgg tcgcaaaata
     4321 ctgcagattc tcgcgtaccg tcaggtcgcc atagaccgct tgctcttgtg tcatatagct
     4381 gatgcgcgag cgcaggccgg ccgagccggc aggcttaccg aagacccgta cgctgccgct
     4441 tgtcaaacgc tgacgtccga cgatagtacg gatgagcgtc gttttgccgg ccccggaggg
     4501 accgatgaaa ccggtgatca cgcctggttt gatctgtaaa tccacagtct gcagggccgt
     4561 gaaacgcccg ccgagcacga cggtcaggtg gcggacatca accacaggag tcatatagta
     4621 cggagtatag cagagcgccg gccaagcata aacggttcgc ttgcccgtcc gtgagcgtta
     4681 cgcttatact ggctgtatga ccgcggatgc ctccattgtc accaatcagc tcagcaagca
     4741 ctatgccggc accaaagtat atgcgctgaa agaccttagt atttccgtgg gcgccggcga
     4801 ggtgtacggc tttctcggtc ccaatggcgc cggcaaaagc acgactatcc gcctgcttat
     4861 gaacttcatc cagccgacga gtggcagcgg ccgcatctta ggcaaagaca tcgtccgcga
     4921 gagcgtcgcc atcaagcggt cggtcggcta cctgtccagc gatatggcga tgtatccgaa
     4981 gctgaccaca gagcagtacc tcgattacat ggctagcctg cagccggcca ccgatgacgg
     5041 gtatcgccat aaactgatgc gccagctgca cgtcgagcct ggcaaacggc tcggtgatct
     5101 gtcacggggc aaccgccaga aggtagcgat cgtgcaggcc tttatgcata aaccgtcggt
     5161 actgatactg gacgagccta gcagcggcct cgacccgctc atgcaggagg cgttctatga
     5221 gctgctgcgg gagtcgcggg cccgtggggc ggccgtcttc atgagcagcc acatcatgag
     5281 cgaggtacaa aaggtatgtg accgcgtcgg cattatccgg gacggccagt tgctggtcga
     5341 gcggaacatc aacgaaatgg aacaagaagc ggctcacacc ctggaaatcc gcttcgcgaa
     5401 caaaccgccg atccgcgagc tggaacggtt ggccggtgtc cagctgaccg ctcacaacga
     5461 acatgaggtt acagttcacc tgcagggtaa actcggaccg ctactgtcgg tactgtcgcg
     5521 gcacgatgtc acccagctgg aggtccgaca gctcgacctt gaagagctgt tcatgcactt
     5581 ctataacgga ggaaagcact catgatgtgg tcgattatcc gctggaccat gcgccagcgc
     5641 cgctggagta tcttctggtg gagtgtgggc atgataggct tcgtggtgct caccctcatc
     5701 ttttatccgt cattcaaaga ccaggccaat cagctgcaga aaagcctcgg taacataccg
     5761 ccgagcgcca tgcagctgtt cggcggtacc gacttcttct ccccggtcgg ctatctgaat
     5821 agccagatca tctacctcat gctgccattg ctgctgacga ttctggccat cggcttgggc
     5881 agcagcctga tcggacgtga ggagcacgat acgaccatcg aatcgttact ggcccggccg
     5941 atatcgcgca gccgtctaat gctcgccaaa gcgtctgccg gggcgctcat cacgctgttc
     6001 gtcgccgcag tcggtaccgc caccatcctg gtcatggtca aagctgtcgg catggccgtg
     6061 ccgctgccga acgtcttgct ggcctgcgct atctgctact tgctggtatt gacgttcggt
     6121 gcggtcgcct tcctgttggc cgctaccggc aaaggccgcg gtgcggcact cggcatcgcc
     6181 accatctatg ccatcgccgg ctatatcatc ggctcgctgg ccggtaccgt ccactggctg
     6241 aaggtaccga gcgtcgtctt tccgtactat tactttcact cgtccgatat attgcgtggc
     6301 acgtttacgt ggggaccggt cgtcttttac gtctgtctga ccgccggctg cgtcattttg
     6361 tcgctggtag cattccgacg acgcgacatc ggttaagcac ttcttttttt ccgtatcgct
     6421 tagttagtga ccggccgccg agtgattatg cggcaaatcc ggcttcccag ggcaagccgg
     6481 atttgccgaa atggccgtaa gcgctcgttt ggcggtagat cggccgggcc aggtccagcc
     6541 gctcgataat ccccttcacg ctggtatcga tgagcgcatc cttaaatgcc tgcagctccg
     6601 cttcgctgac cgtagccgta ccgaaggtgt ctatggtctg catgagcggc agcggttggc
     6661 cgataacata cgccagtccc acttcgcatt tcgtggccag acctttggct acgatattct
     6721 tggcaataaa gcgggctgca taagccccgc tgcggtcgac cttgctggga tccttgccgc
     6781 tgaaagctcc gccacctacg cgggcgtagc caccgtaggt gtcgacgacg atcttacgac
     6841 cggtcaggcc ggcatcgctc tccgggccgg gaatatgcca taggcctgta ccgttgacga
     6901 taatattcgc ctctgccggc aagtcaaatt catacttctt aagcacgggt gcaatgacac
     6961 tttcgatgac atcggcgcgg acttggtcgg ccgatgtcag ctcgtcgtgc gccacggcta
     7021 cgaccacctt ctcgac
//
LOCUS       DASSFD010000033         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1051438, whole genome shotgun sequence.
ACCESSION   DASSFD010000033 DASSFD010000000
VERSION     DASSFD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051438"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFH39_00775"
     CDS             <1..660
                     /locus_tag="VFH39_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6924348.1"
                     /translation="APRFPDTWDLDDMLHPRRAMVLATLATMCALYEAHGIDRYDLVG
                     HSKGGGTAVDTAWFLAEDPDALPGLSVRSVTVVDSVGSTDHSTAELAIRAPFVVHRAL
                     AARHRYKFASDEPPATIRDTLDYALPCLARTGIEGLDCGVTDLRPGIKALRRSNVPVA
                     ALAHVNDEFFPGRRAERHIGELVNAFALGEPVPKAGHFAFQIEPAAVMADHDELLTRM
                     L"
     gene            complement(657..2621)
                     /gene="uvrB"
                     /locus_tag="VFH39_00780"
     CDS             complement(657..2621)
                     /gene="uvrB"
                     /locus_tag="VFH39_00780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET6924349.1"
                     /translation="MSKFELTSDFGPMGDQPAAIAQLTKGIQDGLNHQTLLGVTGSGK
                     TFTMANIIQNIQKPTLVLSHNKTLAAQLYSEFKNFFPNNAVHYFVSYFDYYQPEAYIP
                     RSDVYIEKDSQINEEIDRLRHAATDALLSREDVLIVASVSCIYGIGSVEDYDGLSLKV
                     AKGERRVRDKLLRQLTDIQYQRNDIDFHRSTFRVRGDVVDIYPAGEELAYRLEFFGDE
                     IDRITQIDPLTGEILGDRDRLKIFPSSHYVTPQDKLKIALGKIEHELEERVALFKREG
                     KLLEAQRIEQRTRFDLEMLEETGFVKGIENYSRYLTNREPGEQPATLLDYYPDDYLLL
                     VDESHMTIPQIRGMYNGDRARKEVLVDYGFRLPSALDNRPLTFTEFERHVNQAVYVSA
                     TPAEYELSRSPEPAQQVIRPTGLLDPPIEVRPIEGQIDDLLAEIKDTVAKHQRVLVTT
                     LTKRMSEDLTEYLQELNLKVAYLHSDVDTLERTDILRDLRLGVYDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSEQALIQTVGRAARHVEGHVIMYADRITRSMQTTIDETT
                     RRRAIQEQYNEEHGITPTGIQKAIEERMSQALPEEAKRAKLDLKKIPKDEYGSLIKDL
                     TAQMDLAAANLQFEQAAELRDLITQLKENS"
     gene            2644..2850
                     /locus_tag="VFH39_00785"
     CDS             2644..2850
                     /locus_tag="VFH39_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924350.1"
                     /translation="MIQILINGFDVFEGVGKRFAVSELLRIRRLIIATGGLCQSDTVV
                     IITYFRLIKVRANARIRIGGILIM"
     gene            complement(2919..3299)
                     /locus_tag="VFH39_00790"
     CDS             complement(2919..3299)
                     /locus_tag="VFH39_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924351.1"
                     /translation="MKEAKMKSTSTQAGFAAIEAILILVIVAIIGGTGWFVWHTKQAT
                     DQSLTNTASAQPGIAKANKAKTTTKKSATTTPASSTSTKYLIIKEWGVEIPLTSELAD
                     AYYLGPKPLLAMKALHMWSLVQSL"
     gene            complement(3296..3994)
                     /locus_tag="VFH39_00795"
     CDS             complement(3296..3994)
                     /locus_tag="VFH39_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924352.1"
                     /translation="MTTQKQSGFASLEIVLLAVVVLVLGGTGWFVWHVKRTADRSLAN
                     TVTSAQGQATHVSGGGEAPGSSTRQLTLDHADVTVTIPVDWAATSPSGPFKLTAPSAA
                     DGLDRAEVAPADAAAAQAGSAFGLQVTVTRYDDTNLTAWYWHTYVGEGGPTAEDVTSA
                     STIHGYDGFSRTQTNHNYTDKWYVLHKDSTAVVVYARMLSTHYAATGVIDERDDYTMY
                     QPQVDQIVQSINIK"
BASE COUNT          724 a         1223 c         1285 g          994 t
ORIGIN      
        1 gccccgcggt tccctgacac ctgggacctc gatgacatgc tgcatccgcg gcgggctatg
       61 gtactcgcta cgctcgccac catgtgtgcc ctgtacgagg cgcatgggat tgaccgctac
      121 gacctggtag gacactccaa aggtggcggc acggcggtgg acacggcttg gttcctggca
      181 gaggatcccg acgcactacc cgggctgtcg gtgcggagcg taacggtggt cgattcggtc
      241 ggttcgaccg atcacagcac cgccgaactg gctatccggg cgccattcgt cgtacaccgg
      301 gccctggccg ctcgccatcg ctacaaattt gccagcgatg aaccgccggc gaccatccgc
      361 gacaccttag attacgcctt accctgcctg gcacggaccg gtatcgaagg cttggactgc
      421 ggggtgaccg acctgcggcc gggtatcaaa gcgctgcgac gcagtaacgt tccggtcgcc
      481 gccctggcac atgtcaacga cgagttcttc ccgggccggc gcgctgagcg gcacatcggc
      541 gagctggtga atgcgttcgc cctgggtgag ccggtgccga aagccggcca cttcgcattc
      601 cagattgagc ccgcagccgt catggctgac catgacgagc tccttacgcg aatgctttag
      661 ctgttttctt tcaactgggt gatcagatcg cgcagttcgg cggcctgttc gaactgcaag
      721 ttggcggcgg ccaggtccat ctgagccgtc aggtccttga tgaggctgcc atactcgtcc
      781 ttcggtatct ttttgaggtc gagctttgcc cgtttggctt cctcgggcag cgcctggctc
      841 atgcgctcct cgatggcttt ttggatgccg gtcggggtga tgccgtgttc ctcgttgtac
      901 tgctcctgga tggcacggcg gcgggtcgtt tcatcgatgg tcgtctgcat gctgcgggtg
      961 atgcggtcgg cgtacatgat gacgtgccct tcgacgtgac gggcggcgcg gccgacggtc
     1021 tggataagcg cctgctcaga gcgcaagaag ccctctttgt cggcgtccag gatggccacc
     1081 agtgatacct ccggcaggtc gaggccttcg cgcagcaggt tgatgccgac cagcacatcg
     1141 tagacgccca ggcgcagatc gcgcaggatg tcggtccgct ccagggtgtc gacgtcgctg
     1201 tgcaggtagg cgaccttcag gttcagctcc tgcaggtact cggtgaggtc ctcgctcatg
     1261 cgtttggtca acgtggtcac cagcacccgc tggtgcttcg cgaccgtatc ttttatctcg
     1321 gccagcaggt cgtcaatctg accctcaatc ggccggacct cgatgggcgg gtcgagcaga
     1381 ccggtcggcc ggatgacctg ttgggccggc tccggactac ggctcagctc gtattccgcc
     1441 ggggtggcac tgacgtacac cgcctggttc acatgccgct cgaactcggt gaatgtcagc
     1501 ggtcggttgt cgagggcgct ggggaggcgg aagccgtaat ccaccagcac ttctttgcgg
     1561 gcccggtcgc cgttgtacat accgcggatc tgcggtatgg tcatgtggct ttcgtccacc
     1621 agcagcaggt agtcgtcggg gtagtagtcg agcaaggtgg ccggctgctc gccaggctcg
     1681 cggttggtga ggtagcggct gtagttctca atccccttca cgaaaccggt ttcctccagc
     1741 atttcgaggt cgaagcgggt ccgctgctcg atgcgctggg cttccagcag ctttccctcc
     1801 cgcttgaaca gcgccacgcg ctcctccagc tcatgttcaa tcttgccgag cgctatttta
     1861 agcttgtcct ggggtgtcac gtagtggctg gacggaaaga tcttcaagcg gtcgcggtcg
     1921 ccgagtatct cgccggtcag cgggtcgatc tgggtgatac ggtcgatctc gtcgccgaag
     1981 aactccaggc gataggccag ttcttcaccc gccggataga tatccaccac gtcgccgcgc
     2041 acccggaaag tgctgcgatg aaagtcgatg tcgttccgct ggtactggat gtcggtcagc
     2101 tgccgcagga gcttgtcacg cacgcggcgc tcgcctttgg cgaccttcag cgacagcccg
     2161 tcgtaatctt cgacgctgcc gatgccgtag atgcagctga cgctggccac gatgagcaca
     2221 tcctcgcggc ttaagagcgc atcagtggcg gcgtggcgca gccggtcgat ctcttcgttg
     2281 atctggctgt ctttttcgat gtatacatcc gaacgcggga tgtaggcctc cggctggtag
     2341 tagtcgaagt agctcacgaa gtagtggacg gcgttattgg ggaagaagtt cttgaactcg
     2401 ctgtacagct gcgccgccaa cgtcttgttg tggctcaaga ccagcgtcgg cttctggatg
     2461 ttctggatga tgttggccat ggtaaacgtc ttgccgctgc cggtcacgcc gagcaacgtc
     2521 tggtggttca gcccatcctg aatgcccttg gtcaattggg caatggcggc cggctggtca
     2581 cccatcggcc cgaaatcact ggtcagctca aacttactca cctacacatt gtaacaatac
     2641 actttgattc aaatcctaat taacggcttt gatgtctttg aaggcgtcgg aaaacgcttt
     2701 gcggtcagcg agttgctgcg aattcgccgt ctgattatcg ctacaggcgg cctgtgccag
     2761 agcgacaccg tagtaataat cacctatttt cgtctgatta aagtccgcgc caatgccaga
     2821 atccggattg gcggcatttt gatcatgtaa cgtcacggtt tcgcgtgata tattggcaac
     2881 tcctgttgcg tctggggcgc accgtgcatc gaggctcgtc aaagactttg taccaagctc
     2941 cacatatgca gtgccttcat agccaggagt ggttttgggc ctaaatagta ggcatctgcc
     3001 agttcggacg taagtggtat ctccactccc cattctttta ttatcaggta cttcgtggag
     3061 gtactggatg ccggtgtagt cgtagccgac tttttggttg tcgtcttggc cttgtttgct
     3121 ttcgctatgc caggctgggc gctggcagtg ttcgtcagtg actgatcagt cgcttgtttg
     3181 gtgtgccaca cgaaccagcc ggtgccgcct atgattgcta cgattactag tattaatatc
     3241 gcttctatag ctgcgaagcc ggcttgcgtg ctcgttgatt tcatttttgc ttccttcatt
     3301 taatgtttat cgattgcact atctgatcca cctgcggctg atacattgtg tagtcatcgc
     3361 gttcgtctat cactccggtg gcggcgtaat gcgtcgacag catcctggca tacacgacga
     3421 ccgctgtcga atccttgtgc agcacatacc atttgtcggt gtaattatgg ttggtctggg
     3481 ttcggctgaa cccgtcgtac ccatggatgg tcgaggcgga ggtcacatct tccgccgtcg
     3541 gtccgccttc gccgacgtac gtatgccagt accaggccgt cagattggta tcgtcgtacc
     3601 gtgtcaccgt cacttgcagg ccgaaggccg agccggcctg ggcggctgcg gcatccgcag
     3661 gtgccacttc tgcccggtca aggccgtccg ccgccgatgg cgccgtcaac ttaaacggtc
     3721 cggaggggga ggtggctgcc caatcgaccg gtatcgtcac tgtcacatcg gcatgatcaa
     3781 gcgtaagttg ccgcgtcgaa ctcccaggcg cttcgccgcc accggatacg tgcgtcgcct
     3841 gcccctgggc actggtaacg gtattggcca gggacctatc ggccgtccgt ttgacatgcc
     3901 acacgaacca gccggtgccg cccagcacca ggacgactac tgcgagcagt acgatttcaa
     3961 gactggcgaa gccggattgt ttttgtgtgg tcactgtgtt gccgcttagc gcttatgctt
     4021 gccagtatga attagcgttg cgcttcggtc aacacgagca acgggttgct cagcaggcga
     4081 tttttgagat cttgagtgag ctcaggtcga cggtgaccgg aacgatacgt atggtgtggc
     4141 ggtcgggcag gcgcagattg acgttcacaa gcgccgtaga ggcattccgc ttagctaaga
     4201 aggccgtgtc gtaggtcgcg gcttcg
//
LOCUS       DASSFD010000034         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1683544, whole genome shotgun sequence.
ACCESSION   DASSFD010000034 DASSFD010000000
VERSION     DASSFD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683544"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..323
                     /locus_tag="VFH39_00800"
     CDS             36..323
                     /locus_tag="VFH39_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924353.1"
                     /translation="MNNSISLLLLLTGSASVVAALPQVVQLMKVKTSRELSLVSWTVW
                     LIYQAVSVAYSVHIKAVAYVVINALWVCFYTAMIVLIIRYRKNSVRTRGRA"
     gene            323..913
                     /gene="def"
                     /locus_tag="VFH39_00805"
     CDS             323..913
                     /gene="def"
                     /locus_tag="VFH39_00805"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6924354.1"
                     /translation="MLLKLYQTGQPILRQPAKRVTKQQLASKHMQDVIDFMVDTLRDA
                     PGVGLAAPQVGESLQIIIIDDKAEYHEAVPPELLKEQRREPVPLKVLVNPTLEVVDPE
                     TALYFEGCLSVDGYTGAVARYKSVKVTGWDRDGNDVALTAQGWFARILQHECDHLLGK
                     LYIDVMLPDSFCSVKNFQLLYRKSLEADLRATFGQR"
     gene            939..1532
                     /locus_tag="VFH39_00810"
     CDS             939..1532
                     /locus_tag="VFH39_00810"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6924355.1"
                     /translation="MAKVKQTTNGKIKYFEDQFDDEDVLYVFRKHPVVMRKGLVFGMA
                     AWLVGPLYVLILTYTQPNNPPSLGLFGLALLASIVLGSLIFMPWYISWYFSVFIVTDQ
                     RFIQITQKGLFKRSVVDMSLNQIQMINYEIAGLQETLLGFGTIMMQTFVGDLVIHDVH
                     HPAKTQKKLVGILREQGVMTSSPPAMSQQLITDEQEN"
     gene            1516..2652
                     /locus_tag="VFH39_00815"
     CDS             1516..2652
                     /locus_tag="VFH39_00815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6924356.1"
                     /translation="MSKKTNGKRRLLKNRIRVPQRVRNMRLPKVSLRGRTAESDAKLH
                     EAIQNLPRITNETVAEHREEVLSSARKYIYPLSHSKRRVVSITVSLLAVAVVVFLAYC
                     LTALYKFQSTNTFVYGVTQVIPFPVARAGSSFVSYESYLFELRHTMHYYENEAQPREN
                     FGNPAYKQHLQQLKKAAMNQVIQDAYVKKLASQHHISVSNQEVNNEVALVRSENRLGS
                     NDQVFRDVLSEFWGWTPADFKRELKSQLLAQKVATVLDTQTAARAQDALHKLNTGADF
                     AALAKQVSDDTSTRDNGGAYGIAIDKSNRDIAPQVVDALFAMNPGQHSGVIDTGYTLE
                     IVKLLDVQGDKVHAAHIAFNIKPIDQFVKPLEAQKPVHKYISVQ"
     gene            complement(2641..>3562)
                     /locus_tag="VFH39_00820"
     CDS             complement(2641..>3562)
                     /locus_tag="VFH39_00820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6924357.1"
                     /translation="SLTVLRLISLVSYLLTLPAIYFTGKLATGDKRSGMLAAILLGLS
                     PFMLWYAMRGTMYAPLVLMTVLNQLCFLLVLRKKAWAWPLYVLTAATGLGLHYFFITL
                     LVLQWWFYFIKRREYSRTHKLLMAGSAAALLIGYVLWMHYLSDAIHIFAALPYAGKPS
                     ATNAFIIFVQYLFGFQSVTTITFVIAFWPLLVIVALLAVQKYARPPAGIQYATFTAVA
                     LILGVFILSWIWRSLFLASYLIIGLPAFFLLVSWYLVALELNILAWARTALVVVMAVM
                     LLIELLHPVRAVNEDYLGSLALPRSTLLLH"
BASE COUNT          848 a          980 c         1073 g          661 t
ORIGIN      
        1 tagaaacaag cgggtcggct tggtatgatt agcaggtgaa caattcgata tcgctcttgt
       61 tgctgttgac cggatcggcg tcggtggtgg cggcgttgcc gcaggtggtg cagcttatga
      121 aggttaagac atcgcgcgag ctcagcctgg tcagctggac ggtctggctc atctaccagg
      181 cggtgtcggt cgcgtactcc gtgcacatca aggccgtggc ctatgtggtc atcaatgcgc
      241 tgtgggtctg tttttatacc gcgatgatcg tgctgattat ccgctaccgc aagaactcgg
      301 tacgcaccag gggtagggca taatgctgct caaactgtat cagaccgggc agcccatact
      361 gcggcagccg gccaaacggg tgaccaagca gcagctggcc tccaagcaca tgcaggacgt
      421 catcgacttc atggtcgata cgctgcgcga cgcgccgggc gtggggctag cggcaccgca
      481 ggtgggcgag agtctgcaga taatcatcat cgacgacaaa gccgagtatc acgaggccgt
      541 gccgccggag ctgctaaagg agcagaggcg tgaaccggta ccgctgaaag tgctggtaaa
      601 cccgacgttg gaagtggtgg atccggaaac cgcgctgtac ttcgaaggct gcctgagtgt
      661 agatggttat accggggcgg ttgcccggta caagagtgtg aaagtcactg gttgggaccg
      721 ggacggcaat gacgtcgcgc tgacggccca gggctggttt gctcgcattc tgcagcacga
      781 atgcgaccac ttgctcggca agttgtacat agacgtcatg ctgccggata gcttctgcag
      841 cgtgaagaac ttccagctgc tgtaccgcaa atcgctcgaa gccgacctcc gtgctacttt
      901 cggtcagcga tagctctcca atctgataca atacgctcat ggctaaggtc aaacagacca
      961 cgaacggcaa aatcaaatac ttcgaagacc agttcgacga cgaagacgtg ctgtacgtct
     1021 tccgcaagca ccctgtggtg atgcgcaaag gactcgtctt cggtatggct gcctggctgg
     1081 tggggccgct gtatgtgctc attttgacct acacgcagcc caataatccg ccgagcctcg
     1141 gactgttcgg gctggcgctg ctggccagca tcgtgctggg cagtcttatc ttcatgccgt
     1201 ggtacatcag ctggtacttc agcgtgttca tcgtgaccga ccagcggttc attcagatca
     1261 cccagaaagg gttgttcaag cgcagcgtgg tggacatgag cctgaaccag atacagatga
     1321 tcaattacga gatagccgga ctgcaggaaa cgctgctcgg atttggtaca ataatgatgc
     1381 agacattcgt aggggatttg gtgatacacg acgtgcacca tcctgctaaa acgcagaaga
     1441 aactggtggg catcctgcgt gagcaggggg taatgacctc cagcccaccg gcgatgagtc
     1501 agcaactaat taccgatgag caagaaaact aacggcaaac gccgactact gaaaaaccgg
     1561 atccgggtac cgcagcgggt gcgcaatatg cgtctgccga aggtctctct gcgtggccgc
     1621 accgccgaga gcgatgccaa gctgcacgag gcgatccaga acctgccgcg catcaccaac
     1681 gaaacggtag ccgagcaccg cgaagaggtc ctgagcagcg cccgtaaata catttatccg
     1741 ctcagtcaca gcaagcggcg cgtggtcagc atcaccgtca gcctgttggc cgtggccgtg
     1801 gtggtatttc tggcgtactg cctgaccgca ctgtacaaat tccagtcgac caacactttc
     1861 gtgtacggcg tgacccaggt gataccgttc ccggtggccc gggcggggtc gtcgttcgtc
     1921 agttacgaaa gttatctgtt cgaactgcgc cacaccatgc attactacga aaacgaggcc
     1981 cagccgcgcg aaaatttcgg taatccggcc tacaagcagc acctgcaaca gctcaaaaaa
     2041 gctgccatga accaggtgat tcaggacgcc tatgtcaaaa agttagccag tcagcaccat
     2101 ataagcgtca gtaaccagga agtgaacaac gaagttgcgc tggtgcgcag cgagaaccgg
     2161 ctgggcagta atgaccaggt gttccgcgac gtgctgagcg agttctgggg ctggacgcca
     2221 gccgacttca agcgggagct gaagagccag ctgctggcac agaaggtggc caccgtttta
     2281 gatacccaga cggccgcccg cgcacaggat gcgttgcata agctcaacac cggtgccgat
     2341 ttcgccgccc tggccaagca agtgtccgac gacacgtcca cccgggataa cggcggcgcc
     2401 tacggtattg ccatcgataa gtccaaccgc gacatcgccc cgcaggtggt cgacgcctta
     2461 ttcgctatga atcccggtca gcacagcggt gtcatcgata ccggctacac gttggaaatc
     2521 gtcaaacttc tggacgtgca gggcgacaaa gtccatgccg cgcacatcgc cttcaacatc
     2581 aagcccatcg accagttcgt gaagccgctc gaggctcaga aaccggtaca caagtatata
     2641 tcagtgcagt aacagggtgg agcggggcag cgcgagactt cccagatagt cctcgttgac
     2701 cgctcgcacc gggtggagca gctctatcag caacatcacg gccatcacca ctaccagggc
     2761 agtgcgggcc caggctaaaa tgttcagttc caacgctacc aggtaccagc tgacgagcaa
     2821 aaagaaggcc ggcagcccga tgatgaggta actggccaga aacagcgaac gccagatcca
     2881 gctgaggata aagacaccga gtatgagcgc gacggccgta aaggtggcgt actgaatacc
     2941 cgcaggcgga cgtgcatatt tctggacggc cagcagcgcc acgatgacca gcaggggcca
     3001 gaaggcgatg acgaaggtga tggtcgtgac cgactggaac ccgaacaggt actgtacaaa
     3061 gatgatgaag gcgttggtag cggacggttt gccggcgtac ggcagcgcgg cgaatatgtg
     3121 tatggcgtcg gacaggtagt gcatccaaag cacgtagcct atgaggagcg cggcggcact
     3181 gccggccatt agcaatttgt gggtgcggct atattcacgg cgcttaatga aatagaacca
     3241 ccactgcagc acgagcaggg ttatgaagaa gtagtgcagc cccaggccgg tggcagcggt
     3301 gagtacatag agcggccagg cccaggcttt cttgcgcagc accagcagga aacagagctg
     3361 attcaggacc gtcataagca ccagtggggc gtacatggta ccgcgcatgg cataccacag
     3421 catgaacggc gacaggccga gcaatatggc ggccagcatc ccgctgcgtt tgtcgccggt
     3481 tgccagcttg ccggtaaagt atatggccgg caaggtaagt aggtatgaca cgagcgaaat
     3541 cagccggagc accgtcaggc tg
//
LOCUS       DASSFD010000035         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_809464, whole genome shotgun sequence.
ACCESSION   DASSFD010000035 DASSFD010000000
VERSION     DASSFD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809464"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFH39_00825"
     CDS             <1..415
                     /locus_tag="VFH39_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924358.1"
                     /translation="MHHLPDPSPEFAEFARVLKEDGWAIIEVANYSHARNRVKHLLRG
                     KRLPSKPVDIRSPQNRREDEIPFVNHNPSTVIRQLAHAGLHVEKILSVSNLRSPRLKK
                     MVPHSVMLAAEKAMQRPLASMYFGPSIFLLARKSK"
     gene            445..2481
                     /gene="recG"
                     /locus_tag="VFH39_00830"
     CDS             445..2481
                     /gene="recG"
                     /locus_tag="VFH39_00830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6924359.1"
                     /translation="MELSDPLTNVKGVGPALAGLLARLGLYTVEDIIFYYPRAYDDYS
                     VVTPVAQLKPGAVTIQAHIVSAKGRYVRRGMHVTEAVAADDSGSVRLVWFNQPYRESA
                     LKRSADYFISGIFELSHQRFAIQNPAVELVSDFPLNTARIVPRYRETKGLKSRQIRQI
                     VEQLLPVIQGLGETLPAWLVEKYRLMPYAEAVEQMHFPSSAEQLTAAKHRLGFEEVFA
                     LILAGLMNRLDYEGEQAPAVPFDENVARSFVKALPFDLTPDQRRVVWQIYQDMQATNP
                     MNRLVEGDVGSGKTVVAAMAGLMVLHNGHQAALMAPTELLARQHAETLRQLLEPSGYG
                     DQVGLLVGSLSARQKETARQRIADEHIRFMVGTNALIQESVDMHALELVVIDEQHRFG
                     VDQRKALQLKAGHMPHVLSMTATPIPRSLALTLYGELDISILAHKPPGRQPTTTKLVP
                     PSDRPALYKEIDAELAAGRQMFVVCPLITDSEMSDAASAESVYERFRSREFKHHRVGL
                     LHGRLKAGEKNEIMDAFVRHELDILVSTTVIEVGVDVPNASIMMIEAAERFGLAQIHQ
                     LRGRVGRGGHPGYCYLMLSDTSEPSRRLRALERSQDGFKLAELDLELRGPGAIYGTLQ
                     HGQLDLRIASLADTRLLAEARQAANVFLVSGDDLVQYSRLAATVNRLRAVTNLN"
     gene            2596..3393
                     /locus_tag="VFH39_00835"
     CDS             2596..3393
                     /locus_tag="VFH39_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924360.1"
                     /translation="MRHFDTWFGAHQKIDRTARKHLDAVLPDSGFPKVRLITRFEGQD
                     GPDGIKRKNPAKDEPWHYIDPFDANDDQILGVIGSHYEQLVTALREHDEVRAAFEAAW
                     LSHAIVDGLTPAHHYPYEAELIKLRGGKGIESRTTPKEKLILPGDTLPKQLNNNWKMW
                     GDKGLLSTHFAFEWGVAAIIAPLRLRRSLPTAEQMNQARRLGAAKLFHKRARQIAELR
                     MYDQFYRSGWTPRLARQVRRELAPTIINTVTLAWYVAAMEASRKGNV"
     gene            3390..3920
                     /locus_tag="VFH39_00840"
     CDS             3390..3920
                     /locus_tag="VFH39_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET6924361.1"
                     /translation="MMRIIAGDLKGRQFESPHAQRTHPMADKVRGALFNALGDIEGLT
                     VLDAFAGSGALGYEAVSRGAAHVMAIDIDKRAQHAIAANIKALGIGSKMKLIKANASG
                     WSDNNPDASFDIVIAAPPYDDLQPKLMAKLIRHAKPEGLFILDWPGKMELPDLPGLEL
                     LTAKNHGDAQIAFYRA"
     gene            3972..>4266
                     /locus_tag="VFH39_00845"
     CDS             3972..>4266
                     /locus_tag="VFH39_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924362.1"
                     /translation="MKPVHIHFVCRGNTFRSRLAAAYMSTLVDSGFTVSSSGVDTAHA
                     PVRTVKSYTSRLAHKHNLKYQLTTPKVQTDDQLLASADVIVFMAKDVYDSAVQE"
BASE COUNT          903 a         1288 c         1256 g          819 t
ORIGIN      
        1 gatgcaccac ctgcctgacc cctcgccgga gttcgccgaa ttcgcgcgcg tgctcaaaga
       61 agacggttgg gcgatcatag aggtagccaa ctactcgcac gcccgcaacc gcgtcaagca
      121 cctgctcagg ggcaagcgcc ttccgtccaa accggtcgac atccgcagtc cgcagaaccg
      181 ccgcgaagac gaaatcccgt tcgtgaacca caacccgagc accgtcatcc gtcagctggc
      241 ccatgcgggc ttacacgtcg aaaagatcct gtccgtttcc aatctgcgca gccctcggct
      301 caagaagatg gtgccacact cggtaatgct ggctgctgaa aaagccatgc agcgaccatt
      361 ggcgtccatg tacttcggtc ccagcatctt cctgttggcc cgcaagagca aatagtctac
      421 tccttcggct atactggaaa cagcatggag ctgtctgatc cactgacgaa tgtaaaaggt
      481 gtcgggccgg cgttggccgg gctgctggcg cggctgggcc tgtataccgt cgaagatatt
      541 attttttact accctcgtgc ctatgacgat tattcagtgg tgacaccggt agcgcagctc
      601 aagccgggtg cggtgaccat ccaggcgcac atcgtatctg ccaaaggccg ctacgtacgc
      661 cggggaatgc acgtcacaga ggcggtagcg gccgatgaca gcggcagtgt ccgcctggtc
      721 tggtttaatc agccgtatcg cgaatcggcg ctcaagcgat cggccgacta tttcatatcc
      781 ggcatcttcg agctgagcca ccaacgtttc gccattcaga accccgccgt agagctggtt
      841 agtgatttcc ccctcaatac ggcgcgcatc gtaccgcgct atcgcgagac gaaaggactg
      901 aagtcgcggc agatcaggca gatcgtcgag cagctgctgc cggtgatcca ggggctgggt
      961 gagacattac ccgcctggct ggtagagaag tatcggctga tgccctacgc cgaagcggtc
     1021 gaacagatgc attttccgtc atcggccgaa caactgaccg ccgctaagca tcgcttaggt
     1081 ttcgaggagg tctttgcgct catactggcg gggctcatga accgcctcga ctacgagggg
     1141 gagcaagcac cggccgtacc gttcgacgag aacgtcgcgc gcagcttcgt caaagccttg
     1201 ccgttcgatt tgacgcccga ccaacggcgg gtggtatggc agatatatca ggacatgcag
     1261 gccacaaacc ctatgaaccg attggtggaa ggtgatgtcg ggtccggtaa gacggtcgtg
     1321 gcggctatgg ccggcctgat ggtgctgcac aacggacacc aggcagcgct gatggcgcct
     1381 actgagctgc tggcccgaca gcacgccgag acactccggc agctgctgga gcctagcggc
     1441 tatggtgacc aggtcggcct gctggtgggc agtctgagtg cgaggcagaa ggagacggcc
     1501 agacagcgga tcgcggatga gcatatccgc tttatggtgg gcaccaatgc gctcatccag
     1561 gagtcggtcg acatgcacgc gctggaactg gtggtcatag acgagcagca tcgctttggc
     1621 gtcgatcagc gcaaggcctt gcagctgaaa gccggccata tgccgcacgt gctcagcatg
     1681 accgcgacgc ctataccgcg ctcgctggcg ctgacactct atggcgaact ggatatttcc
     1741 atattggctc acaagccgcc cggtcggcaa ccgactacca ccaagctcgt gccgccctcc
     1801 gatcgacctg cgctctacaa ggagatcgat gctgaactgg cagccggtag gcagatgttc
     1861 gtcgtctgcc cgctcattac ggattccgag atgtcggacg cggcctcggc cgagagcgtc
     1921 tacgaacgct tccgcagccg cgaattcaag catcaccggg ttggcttgct gcatgggcgc
     1981 cttaaggccg gcgagaaaaa cgagataatg gatgcgttcg tccggcatga actggatatc
     2041 ctggtgtcca ctacggtcat tgaggtcggt gtcgacgtcc cgaatgccag tatcatgatg
     2101 attgaggcgg cggagcgttt cgggctggca cagattcacc agctgcgggg cagggtgggc
     2161 cgaggcggtc acccgggtta ctgttacctt atgctcagcg atacatcgga gccgagccgc
     2221 cgactgcggg ctctggaacg tagccaggac ggcttcaaac tcgccgaact ggacctggag
     2281 ctgcgcgggc ccggtgctat ctatggcacg ctgcagcacg ggcaactgga cctgcgcatc
     2341 gccagtctgg ccgatacccg tctgctggcg gaagcgcgcc aggctgccaa cgtattcctg
     2401 gtttcaggcg acgatttggt acaatatagc cgtcttgccg cgaccgtcaa ccgtctgcgc
     2461 gcggtcacca atctcaatta gtcataaagg taaattattc gatgtcacta gtctcggccg
     2521 tatatacaac atgtatcgtg cacgctccgg aaaggggcgg attttaaagt gtattccggt
     2581 actaccttga cgccgatgcg gcatttcgac acctggttcg gagcgcacca gaaaatcgac
     2641 cgcacggccc ggaaacactt ggacgccgtg ctgccggata gcggctttcc gaaggttagg
     2701 ctgattaccc ggttcgaagg tcaggatggg cccgacggga tcaagcgcaa aaatccggcc
     2761 aaagacgaac cctggcatta catcgacccg ttcgatgcga acgatgacca gatactcggg
     2821 gttatcggca gtcactacga acagctggtg accgcgctgc gcgagcatga cgaggtccgg
     2881 gctgccttcg aggccgcctg gctgtcacat gccatcgttg acgggctgac gccggcccac
     2941 cactatccgt acgaagccga gcttatcaaa ctgcgcggcg gcaagggcat cgaatcccgg
     3001 accacgccca aggagaagct tatcctgccc ggcgatacgc tgcccaagca gctcaacaac
     3061 aactggaaga tgtggggcga taaaggattg ctgtcgacgc atttcgcgtt cgagtggggc
     3121 gtggcggcca tcatcgcgcc tctcaggctc cggcgcagtc tgccgacagc cgagcaaatg
     3181 aatcaagccc gacgactcgg cgcagccaag ttgttccaca aacgagcgcg ccagattgcc
     3241 gaactgcgca tgtatgatca gttctaccgc tccggctgga caccgcgtct ggcgcgccaa
     3301 gtccgccgcg agctggcccc cactatcatc aacacggtta cgcttgcctg gtatgtcgct
     3361 gccatggaag catcccgcaa gggcaacgta tgatgcgcat tatcgccggc gacctcaagg
     3421 gtcggcagtt cgagagccct cacgcgcagc gcacccatcc catggccgat aaggtgcgcg
     3481 gcgcgctgtt caacgcactc ggcgacatag agggtctgac cgtgctcgat gcctttgcgg
     3541 gcagcggtgc gctcgggtac gaggcagtca gccgtggtgc ggcgcacgtg atggccatcg
     3601 atatcgacaa gcgcgcccaa cacgccattg ctgccaatat caaagcgctt ggtatcggca
     3661 gcaagatgaa gcttatcaaa gccaatgctt ccggctggag tgacaacaat cctgacgcct
     3721 cgttcgacat tgtcatcgct gctccgccgt atgacgatct gcagcccaag ctgatggcca
     3781 aactgatcag gcatgccaag ccggagggtc tcttcattct ggactggccg ggtaaaatgg
     3841 agttgccgga ccttcctgga cttgaactgc tgactgcaaa gaaccacggc gatgcccaaa
     3901 tcgccttcta tcgcgcttga agattagctg gaccggaggt agccgcttcg tgtacgctaa
     3961 acataagcgt tatgaaaccc gttcacatcc atttcgtctg ccgcggcaat accttccgca
     4021 gccggctggc cgctgcctac atgtccacgc tggttgattc gggctttacc gtcagctcca
     4081 gcggcgtcga tacggcccac gccccggtac gtaccgtaaa gtcctacacc agccggctgg
     4141 cccacaaaca caacctgaag tatcagctga cgacaccgaa agtacagacc gatgatcaac
     4201 ttttggcaag tgccgacgtt atcgtgttca tggccaagga tgtctacgat tcggccgtcc
     4261 aggaat
//
LOCUS       DASSFD010000036         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1573664, whole genome shotgun sequence.
ACCESSION   DASSFD010000036 DASSFD010000000
VERSION     DASSFD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573664"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFH39_00850"
     CDS             <1..1195
                     /locus_tag="VFH39_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924363.1"
                     /translation="RIARGAAGQPGPFAHQAAVFNLAFTALAVVIAIVGWVLGGDAVN
                     FLDVLVVAAPAGLIWAVPLAIQAGLARTLQHGVYVRTGSVLERLAALQSIAFAQTGII
                     TTGDMQLDAVTAVAPHDKPTVLSIAAALLQGSGSTQARAVGAAATAANLKLRRLKRVR
                     ELTGSVRQATISGTVYYIGDRGSLEANGLVFPPKLKADAKPSLYVVTDEAILGRLTFS
                     DALRPETIQTLERLHDFDIRKTFLMAEGGHAAGEALADTVGIREVIALHTTADKLRTL
                     EAVHKRPMAFVGNGVADATLMSAVDIGISIGARASNAASEAASVIVLRDNLALVAVMI
                     AIARRTFGIARQAVAIVTGLSVLFMILFAIAHFPAIYGVILVLIIETAGLLLALRAHH
                     SKISA"
     gene            complement(1192..1869)
                     /locus_tag="VFH39_00855"
     CDS             complement(1192..1869)
                     /locus_tag="VFH39_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924364.1"
                     /translation="MVDKAKAEVKRGVQWVLMAILLTAFAIFIILLGSELWWRSRHPH
                     DEFSRKFIHITVGSFVAFWPFFLSWQQIRLLAVGFIIVVLLSKWLNIFQAIHSVQRPT
                     WGEIYFAIVVGLITYVTHSKAIYATALLQMSLADGLAAVIGVRYGKRHRYFVFGHPKS
                     LAGTLTFFVVSLAILLGYSLHSHHLSAAYIVVLSLLSALVENLAVGGLDNLIVPLVVA
                     VVLTQIR"
     gene            1876..2163
                     /locus_tag="VFH39_00860"
     CDS             1876..2163
                     /locus_tag="VFH39_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924365.1"
                     /translation="MTERTFQSEFDDRKWSLGNLLPSSPIETVPAVLDQLITSREHSQ
                     VHPSEYVAAMVARGFTEDTAVSAVTEHIELGSVALSDTYMLKLPARRETAA"
     gene            complement(2213..2288)
                     /locus_tag="VFH39_00865"
     tRNA            complement(2213..2288)
                     /locus_tag="VFH39_00865"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2253..2255),aa:Pro,seq:cgg)
     gene            2483..>2686
                     /locus_tag="VFH39_00870"
     CDS             2483..>2686
                     /locus_tag="VFH39_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924366.1"
                     /translation="MTPTVDQLEDLYSDTKTCAKLVGLSYIEDETDGIGRRKHGKGFS
                     YVDSRDQVIADRQLKRRITELVIP"
BASE COUNT          590 a          814 c          743 g          539 t
ORIGIN      
        1 gcgcatagcc cggggggcgg ccgggcagcc tggtcccttt gcgcaccagg cggccgtgtt
       61 caaccttgcc ttcaccgctt tggcggtggt gatcgccata gtcggttggg tcttaggcgg
      121 cgacgccgtc aacttcctgg atgtcctggt ggtcgcagct cccgccggtc tcatatgggc
      181 ggtgccgctt gccattcaag ccggtctggc gcgcaccctg cagcacgggg tctatgtccg
      241 taccggttcg gtgcttgaac gtcttgccgc cctgcagagc atagccttcg cccagaccgg
      301 catcattacc acgggtgaca tgcagctcga tgccgtcacc gccgtagcac cgcacgacaa
      361 gcctaccgta ctgtcgattg cggcagctct gctgcaaggc agtggtagca cgcaggcccg
      421 ggcagtcggc gcggccgcaa ccgctgccaa tctcaagctc cggcggctga agcgggtgcg
      481 cgaactgacc ggttccgtcc gccaggccac cattagtggc accgtatatt acattggtga
      541 ccgcggatcc ctggaagcga acgggctggt ctttccgccc aagttgaaag ccgatgcgaa
      601 gccgtcgctg tatgtcgtaa ccgacgaagc catcctcggc cgcttgacat tcagtgacgc
      661 cctgcgtccc gagaccatcc agacacttga gcgattacat gatttcgata tccgcaaaac
      721 gttcctgatg gcggagggcg gtcatgccgc cggcgaagcg ctggccgata cggtcggtat
      781 ccgggaagtt attgccttgc acaccacagc cgataaactg cgcactctcg aagcagttca
      841 caaacggccg atggctttcg tgggcaatgg cgtggccgat gcaaccctca tgtctgccgt
      901 cgatataggc atcagcatag gcgcccgagc atccaatgct gccagcgaag cagcttctgt
      961 catagtactt agagataatc tggcattggt agcggtcatg attgcaatcg cgcgccgcac
     1021 gttcggcatc gcccggcagg ccgtggcaat cgtgacgggt ctgagcgtac tattcatgat
     1081 tctgttcgcc atcgcccact tcccggctat atacggcgtc atcctggtgc tcatcatcga
     1141 aacggccggc ctgttgctgg ccctgcgcgc ccaccactcc aagataagcg cctaacgtat
     1201 ctgggtcagt accaccgcaa ctaccagcgg tacgatcaga ttgtcgagtc cgcccaccgc
     1261 cagattctcc accagcgccg acagcagcga cagcaccacg atataggcgg cggacagatg
     1321 gtggctgtgc agactataac cgagcaagat ggccagcgac accacgaaga atgtcaacgt
     1381 cccggccagg cttttcggat gtccgaacac gaaataccgg tgccgcttgc cgtagcgcac
     1441 gccgatgacg gccgccagcc cgtccgccag actcatctgc aacagtgcgg tcgcgtagat
     1501 cgctttgctg tgggtgacgt acgttatgag cccgaccacg atagcgaagt aaatctcgcc
     1561 ccaggtcggt cgctgaacgc tgtggatagc ctggaagata ttcagccact tcgacaacag
     1621 cacgacgata atgaagccca cggccagcag gcggatctgc tgccaactta aaaagaacgg
     1681 ccagaaggcc acgaacgacc cgacggttat gtggatgaac ttgcgactga actcgtcgtg
     1741 cggatgccgc gaacgccacc acagttcgct gccaagcaga atgataaaga ttgcgaacgc
     1801 ggtaaggaga atggccatca gcacccattg tacgccacgt ttgacttcag ccttggcttt
     1861 atctaccatg acggcatgac agaacgaact tttcaatccg aattcgatga tcgcaagtgg
     1921 agccttggca atcttttacc ctcctccccg atcgaaacgg tgccggcagt cctcgaccag
     1981 cttataacca gccgggaaca ctcacaagta caccccagtg aatatgtggc cgctatggtt
     2041 gcgcgcggat ttaccgaaga tacggccgtg agtgccgtaa ccgagcatat cgaattaggc
     2101 agtgtcgccc ttagcgatac ctacatgctc aagctgcccg ctcgtagaga aactgcagcg
     2161 taaaatatcc ccaaacaaaa aagccccagg taatcccttg gggctttttt gatggtcagg
     2221 gtgagaggac ttgaacctcc gacctcggcg tcccgaacgc cgcgcgctac caactgcgcc
     2281 acaccctgag agtaatgcga caaacctggc ggtttttctc atcgcgcggg gcatcggaat
     2341 gaatccgccg ctccggctgc tcttttcctc ctcgactttc tgcctaagga tggtttggct
     2401 ggaagtattt tatcagatat acagggatat attaactatt ttacagcgtg gccctgcgat
     2461 tgttcggcat gatatgtgac atatgacacc aacagtcgac caactcgaag acctctacag
     2521 tgacacaaaa acctgcgcta agctggtagg gctcagttat atcgaagacg aaacggacgg
     2581 catcggccgc cgcaaacacg gcaaaggttt ttcgtatgtg gacagccgcg accaggtcat
     2641 agccgaccgg cagctcaagc ggcggattac cgaattggtc attccg
//
LOCUS       DASSFD010000037         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_665284, whole genome shotgun sequence.
ACCESSION   DASSFD010000037 DASSFD010000000
VERSION     DASSFD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665284"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VFH39_00875"
     CDS             complement(<1..1251)
                     /locus_tag="VFH39_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HET6924367.1"
                     /translation="MQEILPPRITGTEMEWSITVPLRNCGQPESSMAMNSCEAGDHVQ
                     YYLDRHMGYMGKNHDFYLANGARLYMDVGEHLEYATPEDDSLMGTVANEIAGEQLLRR
                     VFEKAAARPDSSLEEFSLNKRVIDDSGNVWGYHESYYSPGSAITISEAGLALLGVHLA
                     TRNIFFGAGVLRPDGHYRIAQKAGRLNCDSNSGTMHTTVPVVNLRKEPLTAESVNGQR
                     VHVTSGDATMSPWATFMKLGTTSLVLRLMEAGQHMEWLRLKQSLKAVAAMVTHDTTLR
                     TRLELENGKRLYAHEIQEALLSEARRLRGRRQLPEEEDMVLEEWQRAVDDAAEDPLLL
                     ADRADWAGKLVRLRRYRERHGLKWNDEELRRKDRQWDNIGPKGVGLIMRQTTWREWMP
                     DQTLVDARMATPPGTTRAAVRGHFI"
     gene            1361..1858
                     /locus_tag="VFH39_00880"
     CDS             1361..1858
                     /locus_tag="VFH39_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924368.1"
                     /translation="MNPDAQREMIKDADYSRHDHDLQRRKLEMCRTLSSLVLNEQGYT
                     QWFDESRHGGNNYAARVRRLPPAAGQPLTFRIQVGLAEHERVGESEDTTFVHEYGQVT
                     FLPESEEAMKFAEVSKLVHVRDKAPYPQRVAGDSEAFGGIEYTIAQAGLHAALPSFSA
                     SDPRT"
     gene            1885..>2139
                     /locus_tag="VFH39_00885"
     CDS             1885..>2139
                     /locus_tag="VFH39_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924369.1"
                     /translation="MGKLGLSMPKQIAVNVSKRRHPVEQAESMEPHGCDETELSRSVE
                     AELCDIDEVLEESAACLGEAALVLALEEERQEWLDWRTAYL"
BASE COUNT          417 a          633 c          650 g          439 t
ORIGIN      
        1 tatgaagtgc ccgcgcaccg cagcccgggt agtgccgggc ggggtggcca tccgtgcgtc
       61 caccagtgtc tggtccggca tccactcacg ccaagtcgtc tgccgcataa tcagcccgac
      121 accttttgga cctatgttgt cccactgccg atccttgcgc cgcagctcct cgtcgttcca
      181 tttcaggccg tggcgttcgc ggtagcgacg taaccgcact aattttcctg cccaatcggc
      241 ccggtcggcc agcagcagtg gatcttcggc ggcgtcatcc accgctctct gccactcctc
      301 cagcaccata tcttcttcct ccggcaattg ccggcgcccc cgcaggcgcc gggcctcaga
      361 cagtagggct tcctgaattt catgtgcata caggcgcttg ccgttctcca gttccagtcg
      421 ggtacgcagg gtcgtatcgt gtgttaccat cgcggctacg gccttgagcg actgcttcag
      481 gcgtagccat tccatgtgct ggccggcctc catgagcctt agaaccaacg aagtggtgcc
      541 cagcttcatg aacgtcgccc agggcgacat ggtggcatcg ccgctggtga catgcaccct
      601 ttggccgttg acgctttcgg cagtcaacgg ttctttgcga agattgacga ccgggacagt
      661 cgtatgcatg gtgccggagt tggaatcgca gttgagccgg ccggccttct gggcgatgcg
      721 gtagtggccg tcgggccgca gtacgccggc tccgaagaat atgttacgcg tggcaaggtg
      781 gacgcccagc agtgccaagc ccgcttcgct gatggttatg gcggagccgg gtgaatagta
      841 gctttcgtgg tagccccaca cattgccgct gtcgtcaatg acacgtttgt taaggctgaa
      901 ttcttccaag ctgctgtccg gccgggccgc cgctttttca aatacccggc gcagcaattg
      961 ctcgccggct atttcgttgg caacggtgcc catgagggag tcgtcctccg gtgtggcgta
     1021 ttccaggtgc tcgccgacgt ccatataaag ccgggcgccg ttcgccaggt aaaaatcgtg
     1081 gtttttaccc atatagccca tgtggcggtc gaggtagtac tggacgtggt ccccggcctc
     1141 gcacgaattc attgccatgg aactctcggg ctgcccgcag ttccgaaggg gaaccgtgat
     1201 actccactcc atctcggtgc cggtgatgcg cggcggcagt atctcttgca tatctggagc
     1261 tctattgtac tacgtatcat cctacggcaa atattttgcc ggcagtgcac atgatgctca
     1321 tgcttgcagg gcggcagggt cggggcagat aatatcgggc atgaacccgg atgcccaacg
     1381 agaaatgata aaggacgccg actacagccg gcatgatcac gacctgcagc ggcgcaagct
     1441 ggaaatgtgc cgtacgctta gcagcctggt gctgaatgag caaggctata cacagtggtt
     1501 cgacgaaagc cggcacggcg gcaacaacta tgccgcgcgc gtcaggcgcc tgccgcccgc
     1561 agcgggccag cccctgacgt tccggatcca ggtcggcctg gcggagcatg aaagggtagg
     1621 cgaatcggaa gataccacgt tcgtacacga gtacggccag gtgacgtttt tgccggaatc
     1681 ggaagaagcg atgaagttcg ccgaagtcag caaactggtc catgtgcggg acaaagcgcc
     1741 gtatccgcaa cgggttgcag gtgactccga agccttcggg ggcatagagt acaccatagc
     1801 tcaggccggg ttgcacgccg cactgccgtc gttctctgcc agcgacccgc gcacttgaca
     1861 gtacataacc ataagcgcag aatcatgggt aagttaggat tatccatgcc gaaacaaata
     1921 gccgtgaacg tatcaaagcg tcgccatccg gtagagcagg cggagtctat ggagccacac
     1981 ggctgcgacg aaaccgagct cagccgctcg gtcgaggccg agctttgcga tatcgatgag
     2041 gtgctggaag agtctgcggc ctgtttgggc gaggcagcgc tagtgctggc ccttgaagaa
     2101 gaacggcagg aatggctcga ctggcggact gcatacctc
//
LOCUS       DASSFD010000038         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1544991, whole genome shotgun sequence.
ACCESSION   DASSFD010000038 DASSFD010000000
VERSION     DASSFD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544991"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFH39_00890"
     CDS             complement(<1..464)
                     /locus_tag="VFH39_00890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6924370.1"
                     /translation="MRRASTIEKETVQITTIEDLTGVFESIASTQVAKVKNKVELSKQ
                     FFQLLWKRYSSIRFDPSTRIGSRSDQGKNGRKVFVIISAEAGLSGDIDQRLIEHMLQD
                     YNPKDTDIIVLGSHGAQQLDQHGVHYIRFFQVPESDSYIDVSPVIEAALPYS"
     gene            complement(560..2047)
                     /locus_tag="VFH39_00895"
     CDS             complement(560..2047)
                     /locus_tag="VFH39_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HET6924371.1"
                     /translation="MCDNPAFQRLVEKDNLTGEVVASNSFIIEVKGLEGVRLGAQVLF
                     SDGQRGLVREAYGDRVILFNLDSERMEPGTLAVVQRDELQVPVGKSLVGRVVSPMGEP
                     LDGKGTVRTSTTSGVFNAAPGIMARSMLDTQLASGVAAVDMFFPVVLGQRIAVLGDSK
                     SGKSTFLGQLSANQQGTDRIVVYVLVGKRKVDIEQLLARLRSSGAMQNTIVVLADIFD
                     SLTQSYLAPYAACAMAEHLWYGGQDVIVIYDDLSSHAEAYRQLSLLQEVDPGRDSYPG
                     DMFYAHSSLLERAGKLLKSGKTLTALPVVMTPNDDITAYLSTSIMSITDGQIIFDLNV
                     FRQGIRPAVNAGLSVSRVGGQAQTVRQKLLSSNLFKKLAQYHQAEEFSHFGSSLSKEA
                     AIDLQLGKQIYAALQQPPEERHSLAAQQLILETILLGGGTVDLDVTGLKQEAAKLAPK
                     AKDEKDFDHLEAELLKKFGKKPAAEKSEESPEEKPAEAPKEAANA"
     gene            complement(2040..2528)
                     /locus_tag="VFH39_00900"
     CDS             complement(2040..2528)
                     /locus_tag="VFH39_00900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET6924372.1"
                     /translation="MKIKLDPSVASPQDLQALTRELHDYARWLGTNIIKKQVHARRPA
                     PDQPQLSPAAKAVLHDWCLDHPLTRAGIDQLAVELERYLKSAKVLTITLAAPPTGGIT
                     KPLTGWCRDNISPSVLVRYEFNQNLLGGMVVRYGSHIYDWSFRRQILEGRGKFPEVLR
                     HV"
     gene            complement(2518..>2938)
                     /locus_tag="VFH39_00905"
     CDS             complement(2518..>2938)
                     /locus_tag="VFH39_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924373.1"
                     /translation="NFQHVLNTSAKELQQDLAATAEDIHKLVQKMGTEAVAGELQNYR
                     ARLAELQQKTEADMAGVRDAITAHQAELKAKLEADIAAEKERLLTQIDTKLADAVAAF
                     LLDTLQHEVDLGAQQQYLTKMLEEHKEELKKDLGDEN"
BASE COUNT          496 a          856 c          903 g          683 t
ORIGIN      
        1 tgggagtagg gcagagccgc ctctatgacc gggctgacat ctatgtagct gtcactttcc
       61 ggcacctgga aaaagcggat gtagtgcacg ccgtgctggt cgagctgctg ggcgccatgg
      121 ctgccgagca cgattatatc ggtgtctttg gggttgtagt cctgcagcat gtgttctatg
      181 agccgctggt cgatgtcgcc ggacaggcct gcctcggccg agatgatgac gaacaccttg
      241 cggccgtttt tgccctggtc gctgcggctg ccgatacggg tgctggggtc gaagcggatg
      301 gagctgtagc gtttccagag tagctggaag aactgtttgg acagctccac cttgtttttg
      361 accttggcga cctgggtact ggcgatgctt tcgaacacgc ccgtcagatc ttcgatggtc
      421 gtaatctgga ccgtttcttt ctcaatagtg ctggccctac gcattaccgc cccctacggg
      481 taacgagaca gacctacggt ttttttcatc gcgcgggacg tggaatgaat ccgcgctccg
      541 gctgctcttt tcccttctac tacgcattgg cggcctcctt gggggcttcg gccggctttt
      601 cttccggtga ttcttcggat ttttcggcgg caggcttctt gccgaacttc ttgagcagtt
      661 cggcttccaa atggtcgaaa tccttttcgt ctttggcttt aggagccagc ttggcggcct
      721 cctgtttcag gccggtcacg tccaagtcga ccgtgccgcc gccgagcagg atagtctcga
      781 gtatcagctg ctgggcggcg aggctgtggc gctcctcggg cggctgctgc agagccgcat
      841 atatctgttt accgagctgc aggtctatgg cagcctcctt gcttaggctt gaaccgaagt
      901 gcgaaaactc ctcggcctgg tggtactgcg ccagcttttt gaagaggttg ctgctgagca
      961 gcttctggcg cacggtttgc gcctggccgc cgacgcgcga cacggacaag ccggcattga
     1021 cggccgggcg gatgccctgg cggaaaacgt tgaggtcaaa gattatctgg ccgtcagtga
     1081 tggacatgat gctggtgctg aggtaagcgg taatgtcgtc gttcggcgtc atgaccaccg
     1141 gcaaggcagt gagcgtcttg ccgctcttga gaagtttgcc ggcccgttcg agcaggctcg
     1201 agtgtgcata gaacatatcc cccgggtagc tgtcgcgccc tggatccact tcctgcagca
     1261 gcgacaactg gcggtaggct tcggcgtggc tggacaggtc gtcataaatg acgattacat
     1321 cctgcccgcc gtaccacagg tgctccgcca tggcacaggc ggcatatggc gccaggtatg
     1381 attgggtgag cgaatcaaat atatcggcca gcacgacgat ggtgttctgc atggccccgg
     1441 acgaacgcag gcgcgccagc aactgctcga tgtccacctt gcgcttaccg accaatacgt
     1501 agactacgat gcggtcggta ccctgctggt ttgcgctcag ctgccccaga aaggtacttt
     1561 taccggactt gctgtcgcca agcacggcga tgcgttggcc cagcaccacc gggaagaaca
     1621 tatccacggc cgcgacaccg ctggccagtt gggtatccag catgctgcgg gccatgatgc
     1681 cgggcgcggc attgaacacg ccggaggtag tggaggtgcg gacggtgccc ttaccgtcca
     1741 gcggctcgcc catgggcgac actacccggc cgaccaggct cttgccgacc ggcacttgca
     1801 gctcgtctct ctgcacgacg gccaatgtac ccggttccat cctttcagaa tccagattga
     1861 acagtatgac ccgatcgccg taagcttcgc gtaccagccc gcgctgcccg tcgctgaaga
     1921 gtacctgcgc accgagccgc acgccttcca ggcccttcac ttcgataatg aaactgttgc
     1981 tggcgacgac ttcgccggtc aggttgtcct tttcgactaa gcgctgaaag gccgggttat
     2041 cacacatggc gcaggacctc cgggaattta cctctcccct ccaggatctg ccgccggaac
     2101 gaccagtcgt atatgtggct gccgtagcgc accaccatgc cgccgagcag gttctggttg
     2161 aattcgtagc gtaccagtac gctcgggctg atattatccc ggcaccagcc ggtcaatggt
     2221 ttcgttatgc cgccggtcgg cggggcggcc agcgtaatgg tcaggacttt ggcgcttttg
     2281 aggtaacgct ccaactctac ggccagctgg tcgatgccgg cccgagtaag cggatggtcc
     2341 aaacaccagt cgtgcagtac ggctttcgct gccgggctga gctggggctg atcgggggca
     2401 gggcggcggg cgtgcacctg ctttttgatg atgttcgtgc caagccagcg ggcataatcg
     2461 tgcagttcgc gcgtaagcgc ctgcaagtcc tgcggcgatg cgacggacgg gtcaagctta
     2521 attttcatca cctaaatcct tcttgagctc ttccttatgc tcttcgagca tcttcgtcag
     2581 atattgctgc tgggccccca ggtcgacctc gtgctgcagg gtatcaagca ggaaggcggc
     2641 gaccgcgtcg gccagcttgg tatcgatctg cgtgagcagg cgctcctttt ccgcggctat
     2701 gtcggcttcc agtttggctt tcagttcggc ttggtgggcg gtgattgcat cgcgtacgcc
     2761 ggccatatcg gcttcggtct tttgctgcag ttcggccagg cgggcacggt agttctgcag
     2821 ctcgccggcg acggcttcgg tgcccatctt ctgtactagt ttgtgaatgt cttcggccgt
     2881 tgccgccaga tcctgctgca gttccttggc ggaggtgttc agtacatgct ggaaattg
//
LOCUS       DASSFD010000039         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1429914, whole genome shotgun sequence.
ACCESSION   DASSFD010000039 DASSFD010000000
VERSION     DASSFD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429914"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="VFH39_00910"
     CDS             <1..1965
                     /locus_tag="VFH39_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924374.1"
                     /translation="HTQSHRQTPATGPAFSVRRRPLPRRRTNKDTKDFSLSMSHARKG
                     TQKDIPVAEAKRIVLEKISQGYKVAEATAVVGRKEETYRDWRKTDPEFRAAVDEIRKS
                     KNELQENGRPEVPPFDEFCRDWLKQPLFPHQLRMWDVIEGRKPRDLHPSMDYLPGYRN
                     RVVINVPPEHAKSTTFTVNYVVWLIHRNPDIRVVIMSQGKTLAQRFLGEIKFKLTSPL
                     YREMHLRYGPADGWKSNEAEWSQDAIYVRGKGGDKDPTVQALGLGGQIYGTRADLIIL
                     DDTITTKNAREIDKQMILLEREIESRLPSDQEGGGVLAVLGTRVSPHDLYRELMDVRD
                     ADDDRVWTYFRMPAVLDYGNGDSATWETLWPEKWNGKSLSRRKRGTAWNLVYQQLNVD
                     DDMTFKAEAVDASINGKRFPGPMTREGAGHRPGGMEGLYLVGGLDPAAAGNTAIIIAG
                     FDRETQKRYILDGWNKPNATAEQIINRFKYFTETYGLHEWVIEQNALQRFITQLPELT
                     EFTRARGCKITPHTTNANKYDSDWGIETMAPLFDSCGEVDEKNPNGRWKRTPAKALIE
                     LPSPRQNDWVNDLIQQLTIWQPEGMAQKQKTDLVMALWFTHIAIMRIIRRGRNKQTHL
                     STPYMTPAARKKQQVIDLAAMRRAKQEAIA"
BASE COUNT          493 a          633 c          655 g          381 t
ORIGIN      
        1 cacactcagt cgcacaggca aacgccggcc accgggccgg cgtttagtgt tcgccgccga
       61 ccccttccca ggcggcgaac gaataaagac accaaggact tttcattgtc gatgtcacat
      121 gccaggaagg gaacgcagaa ggatattccg gtagccgagg ctaagcgaat cgtcttagag
      181 aagattagcc aaggctataa ggttgccgaa gctacggccg ttgtcggccg caaggaggag
      241 acctaccggg attggcgcaa gacagatccc gagttccggg ctgccgttga tgagatacgg
      301 aagtccaaga acgagctgca agagaacggc cggccggaag tgccgccgtt cgatgagttc
      361 tgccgggact ggctgaagca gccccttttc ccgcatcagc tacgaatgtg ggatgtgatt
      421 gaggggcgca agccccgaga cctgcatccc tctatggact acctgcccgg ttatcggaac
      481 cgggttgtta tcaatgttcc cccggaacac gccaagagca cgacgttcac cgtcaactac
      541 gtggtgtggc tcattcacag gaacccggac atccgggttg tgattatgtc gcagggcaag
      601 accctggcgc agcggttcct cggggaaatc aaattcaagc ttaccagtcc gctataccgg
      661 gaaatgcatt tacgttatgg gccggctgac ggctggaagt ccaatgaggc cgaatggtct
      721 caggatgcga tctacgtccg gggtaagggt ggcgacaagg acccgaccgt gcaggcgctt
      781 ggcctgggcg gtcagattta tgggacgcgc gccgacctaa tcatcctgga cgacacgatc
      841 accaccaaga acgcccgcga gatcgacaag cagatgattc tgctggaacg cgagatcgag
      901 tcgcggctgc cttccgacca ggagggcggc ggcgttctgg ccgtgcttgg cacgcgggtt
      961 agcccgcatg acctctaccg cgagctgatg gacgttcggg atgcggacga cgaccgcgtg
     1021 tggacgtact tccgtatgcc ggcagtcctt gactacggca atggcgactc ggctacctgg
     1081 gagactctat ggcctgagaa gtggaacgga aagtcactgt ctcgccgcaa gcgcggcacc
     1141 gcatggaatc tcgtttacca gcagttgaac gtagacgacg atatgacctt caaggccgag
     1201 gccgtggacg cgtctatcaa tggtaagagg ttccccggac cgatgacccg agagggtgcc
     1261 ggacaccggc ccggcggaat ggagggcctg tatctcgtcg gcgggctgga cccggcggcg
     1321 gcgggtaaca ccgcaatcat catcgcgggg ttcgaccgcg agacccagaa gcggtacatc
     1381 cttgacggct ggaacaagcc gaacgccact gctgagcaga tcatcaaccg cttcaaatac
     1441 ttcacggaga cctacgggct ccacgagtgg gtcattgagc agaacgctct acagaggttc
     1501 atcacgcagc tgcccgagct tacggagttc accagggcgc gcggctgcaa gataacgccg
     1561 cacaccacga acgccaacaa gtacgactcg gactggggca tcgaaacgat ggccccgctg
     1621 ttcgactcct gcggtgaagt tgacgaaaag aaccctaacg gtaggtggaa gcgcacgccg
     1681 gctaaggcgc tcatcgagct tccctcgcct cgtcagaacg attgggtgaa cgacctcatc
     1741 caacagctga cgatctggca gccggaggga atggcgcaga agcagaaaac cgaccttgtg
     1801 atggccctgt ggttcacgca catcgccatc atgcggatca tccggcgtgg ccggaacaag
     1861 cagacccatc tttccacccc gtacatgacg ccggctgcac gcaagaagca gcaggttatc
     1921 gacctagccg ctatgcggcg cgctaagcag gaggccatcg catgagtgac gttgcgatcc
     1981 cgaccttcat gccgcagttg gttagtcact acacgcgaat gatgtcccgc aacgcgacca
     2041 gggatagaca ccacgagctg atccatcagg ttcgccgggg caacattcgt cagctgttcc
     2101 ccgaggaact gaacttcagt atcagcttcc aagggtcgcc gatctccaac ttcgttgaca
     2161 ta
//
LOCUS       DASSFD010000040         4547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_419379, whole genome shotgun sequence.
ACCESSION   DASSFD010000040 DASSFD010000000
VERSION     DASSFD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419379"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFH39_00915"
     CDS             <1..418
                     /locus_tag="VFH39_00915"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET6924375.1"
                     /translation="KAALLAGDKFKVDTLRLLKSVLLNYKVANGTRDRAMTDDEVINL
                     FSKEAKKRQESADLYVQGGSQEKADKELAEKQLIDEYLPAQLSDDELRALVDEAVAAA
                     GDNPNMGQLIGQVKAKAGAGADGGRIAVLVRERLGQ"
     gene            415..963
                     /locus_tag="VFH39_00920"
     CDS             415..963
                     /locus_tag="VFH39_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET6924376.1"
                     /translation="MILFFGPPGSGKSVQGQLLVQRNGWNWLSTGELFRNSKDPEVLA
                     RLATGELIDDAMTNEVLDTALRAIKDKAYVVLDGYPRNTEQAAWLDEHLPQQGREIKA
                     VIVFEVSEEELVRRLSGRGRDEDKPEVIKHRLDIYYRQTKPVVEFYERLGVPVCRVDG
                     HGSIEEVHERIQQAVEAQNLTA"
     gene            960..1715
                     /gene="map"
                     /locus_tag="VFH39_00925"
     CDS             960..1715
                     /gene="map"
                     /locus_tag="VFH39_00925"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6924377.1"
                     /translation="MMTRVKTKPEIAAMREAGGMLASVLQLLKRELTAGMSTKDLAVM
                     AADELKRLGGKPSFLGYQGFPDVICISVNDEVVHGIPRSDKIIHEGDIVSLDFGVTVQ
                     GMVTDAAISTICGQAKDKRHLKLVQLTEASLLEGIAAVHDGVRTGDIGAAVEAVLSKH
                     KYGIVRDLVGHGVGHELHEDPNIPNYGRAHTGPWLQAGMTIAIEPMATLGTDKVYMAD
                     DGWTILTRDGSLSAHFEHTVLITEDGAEILTTV"
     gene            complement(1712..2209)
                     /locus_tag="VFH39_00930"
     CDS             complement(1712..2209)
                     /locus_tag="VFH39_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924378.1"
                     /translation="MRETLRRVWMAAGLSGAASLAISGCTGASHETPSPDSLTPQQAY
                     REAHCRNMTARPAAQAGEFILTITGHARVPADYGVVVIRNSGSADPLTTAKIFKLSNS
                     GHDETVTGIVDLTSTTPGLSRFDITGIVSPDGYHGQLMTDRTCQYTAYFEPNPSSVTQ
                     PEQPA"
     gene            2311..3552
                     /gene="ftsA"
                     /locus_tag="VFH39_00935"
     CDS             2311..3552
                     /gene="ftsA"
                     /locus_tag="VFH39_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET6924379.1"
                     /translation="MPQSANPHFVGLDIGTSTVRCVVGMLDTSAGNKPSIIGHGSAPN
                     QGMRKGVVVHVDDVAEAIVQAITEAERISGYRITQATVNVNGAHVSGINSKGVIAISS
                     ANREITGEDRYRVEEAATVVQLPPNREIIQFFAKNYSVDGQQNIKDPVGMSGVRLEVD
                     AHIVTASTPSLRNLDFALSKAQVAPQHHTVSSLAATEAVMSRQQKEAGTVLLDIGAGT
                     TNLIVLEDGEVQHVAVLPIGGMHITNDLAIGLKTDLDVAEQVKLQHATLGKATKTTAN
                     VNIGKTTHSFPMDDVQMIVEARVEELLEFVDKELARIKRSRKLPGGVIITGGTAKLPG
                     MAEFAKEHLQLSARIGKLNEEIGGLVDTVEGTDYATVIGLMLLDMLLLPEEPAAAQGA
                     AGKATAGAMGMVQGLLKRLKT"
     gene            3970..>4547
                     /locus_tag="VFH39_00940"
     CDS             3970..>4547
                     /locus_tag="VFH39_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET6924380.1"
                     /translation="MPQVVEPAIETFAKIKVVGVGGAGGAAINRMVEAGIENVEFIAV
                     NTDAQALHHSKAAKKIHIGKDETRGLGAGADPSVGKRSAEESREEIRQALEGADMVFV
                     TIGAGGGTGSGAGPVVAEAAKELDILVVGFATKPFAFEGEKRRKNAEVAIEELRDAVD
                     TLIVIPNDRLLQTIDRNTPLMEAFKVADDVLR"
BASE COUNT          963 a         1326 c         1469 g          789 t
ORIGIN      
        1 gaaggccgcg ctgctcgcgg gtgacaaatt caaagttgac accctgcgac tccttaagag
       61 cgtgctgctg aactacaaag tcgccaatgg tacccgcgac cgggccatga cggatgacga
      121 ggttattaat ctgttcagca aagaggccaa gaagcgccag gagagcgccg acctgtacgt
      181 gcagggtggc agccaggaga aggccgacaa ggaactggcc gaaaagcaac tcatcgacga
      241 atacctgccg gcccagctga gcgacgacga actgcgcgcg ctggtcgatg aagccgttgc
      301 cgcggccggc gacaacccga acatgggaca gcttatcgga caggtaaaag ctaaggcggg
      361 cgccggcgcc gatggcggcc gcatcgcggt gctggtccgt gaaaggctcg gccagtgatc
      421 ctgttcttcg gccctcccgg ttccggcaag agcgtccagg ggcaattgct ggtgcagcgc
      481 aacggctgga actggctcag taccggcgag ctgttccgta actccaaaga tcccgaagtg
      541 ctggcccgcc tggcgacggg cgagctgatt gacgacgcaa tgaccaacga ggtgctggac
      601 accgcgctgc gtgccatcaa ggacaaggcc tacgtggtgc tggacggtta cccgcgcaac
      661 accgagcagg cagcctggct ggacgagcat ctgccccagc aaggccgcga aatcaaagcc
      721 gtcatcgtgt tcgaggtcag cgaagaagaa ctggtgcgcc gcctgagcgg ccgcggccgc
      781 gacgaggaca agccggaggt catcaaacac cggttggata tctattatcg ccagaccaag
      841 ccggtggttg aattttatga acgtttaggc gtgccggtgt gccgcgtgga cgggcacggt
      901 tcgatcgagg aagtgcacga gcggattcag caggccgtgg aagcccagaa tctgaccgca
      961 tgatgacgcg cgtaaaaacc aagccggaga tagccgccat gcgcgaggcc ggcggcatgc
     1021 tggccagcgt gctgcagctg ttgaagcgcg aactgacggc cggcatgagc accaaagacc
     1081 tggcagtcat ggccgctgac gaactgaagc ggctcggcgg caagccgagt tttttgggct
     1141 accaggggtt tccggacgtc atctgcatat cggtgaacga cgaggtggtg cacggcatac
     1201 cccgctccga taagattatt cacgagggcg acatcgtcag tctcgacttc ggcgtgacgg
     1261 tgcagggcat ggtgaccgac gccgccatca gcaccatctg cggccaagcc aaggacaagc
     1321 gccacttgaa gctggtgcag ctgaccgaag cgtcgctgct ggaaggcatt gcggccgtac
     1381 atgacggcgt caggaccgga gacatcggcg cggccgtcga agccgtactg agcaagcaca
     1441 agtacggcat cgtccgcgat ctggtcggtc acggtgtcgg gcacgagctg cacgaggacc
     1501 cgaacatccc gaactacggc cgggcgcaca ccgggccgtg gctgcaagcc ggcatgacca
     1561 tcgccatcga accgatggcc accctcggta ccgacaaagt ctacatggcc gacgacggct
     1621 ggactatcct gacccgcgac ggctcgctca gcgcccactt cgagcacacg gtgctcatca
     1681 ccgaagacgg cgccgagata ttgacgacag tctaggcggg ttgttctggc tgcgtcactg
     1741 agctggggtt gggttcgaag taagctgtgt attggcaggt gcgatcggtc atcagctgtc
     1801 cgtggtaccc gtcggggctc acgattccgg taatgtcgaa ccggcttaat cccggcgtgg
     1861 tgctggtgag atcgacaatg cctgttaccg tctcgtcgtg tccgctgttg gagagcttga
     1921 aaattttagc ggttgtcagc gggtcggcgc tgccggagtt gcggatgacg accaccccat
     1981 aatcggccgg tacccgggca tgcccggtaa tcgtcaggat gaactcaccg gcttgggcag
     2041 ccggacgggc ggtcatgttc cggcagtggg cttcccggta tgcctgctgg ggcgtcagtg
     2101 aatctggtga gggcgtttcg tggctagcac ccgtgcagcc gctgatagcc agcgaggccg
     2161 caccggacag tccggcagcc atccatactc ttcgcagtgt ttcacgcatg atcgtacccc
     2221 atttatatgc ccgtactgta cggcaagcgg ggcggcgagg caactgacag ttgccttcct
     2281 taagacacta gcgttatact actgcataat atgccccagt ctgccaaccc acacttcgtc
     2341 ggccttgata tcggtacgtc tacggtccgg tgtgtcgtcg gtatgctgga cacttcggcc
     2401 ggcaacaagc cgtcaatcat cggtcatggc agcgcgccga accagggtat gcgcaaaggc
     2461 gtcgtggtac atgtagatga cgtcgccgaa gcgattgtgc aggccattac cgaggcggag
     2521 cgcatcagcg gctaccgcat aacccaggcg acggtcaatg tgaacggagc gcatgtgagc
     2581 ggcatcaaca gcaagggcgt catcgctatc agcagtgcca accgcgagat aaccggcgag
     2641 gaccgctacc gggtcgagga agcggcgacg gtggtacagc tgccgccgaa ccgcgagatt
     2701 atccagttct ttgccaagaa ctacagcgtc gacggccagc agaacatcaa ggacccggtc
     2761 ggcatgagcg gcgtccggct ggaggttgat gcccacatcg tcaccgccag tacgccatcc
     2821 ttgcgtaatc tggacttcgc gctcagcaag gcgcaggtgg cgccgcagca ccacacggtg
     2881 tccagcctgg ctgccaccga ggcagtcatg agccgccagc agaaggaagc cgggacggtc
     2941 ctgctcgaca tcggcgccgg cacgactaat ttgatcgttc tggaagacgg tgaggtccag
     3001 cacgtcgcgg ttttgcccat cggcggcatg cacatcacca acgacctggc catcggcctc
     3061 aagaccgacc tggacgttgc cgaacaggtc aaactgcagc acgccacgct tggcaaggcc
     3121 actaagacga cggctaatgt aaatatcggc aagacgacgc acagtttccc gatggacgac
     3181 gtgcagatga tcgtcgaagc ccgggtggaa gagttgttgg agttcgtcga caaggaattg
     3241 gcgcgtatca agcggtcccg taagctgccc ggcggcgtta tcatcaccgg cggcactgcc
     3301 aagctccccg gcatggcgga attcgctaag gaacatctgc agctcagtgc ccgtatcggt
     3361 aagctgaacg aagagatcgg cggcctcgtc gacaccgtcg aaggcacgga ctacgccacc
     3421 gtcatcggtc tgatgctgct cgatatgctg ctgctgcccg aagagccggc agcggcccag
     3481 ggggcggcgg ggaaggctac cgccggggcg atggggatgg tgcaggggct tttgaagcgc
     3541 cttaagacct aacgggcttt ttgtagttga ttgattggag gggcggcaga gttgccggcc
     3601 ctcttacttt tgccttgatg caaaagtaag caaaagatca aggcccgatc gccgctctcg
     3661 gtcgtcagtt gtgccagctt acaaccggtc ggcggactcg cctccggctc aaacatcgcc
     3721 gaccgaccct ccgggcgagt tgtaagcggc acgaacagcc gagccgggaa ctgctctagg
     3781 gccgttttac agtggcggag gttgggtaca aaatttgtgt ggtggcgggt tggcgcggaa
     3841 gggtcgagta cattaggcgg ttgccaaaaa tacagcagtt tcggccagta tacatttgtt
     3901 gaacttattc ggtaaccggg gtatactggc agctataatc cgcttaagta ggagcatgag
     3961 gacagccgta tgccgcaagt cgtcgagcca gctatagaaa cgtttgccaa aatcaaagtg
     4021 gtcggagtcg gcggtgccgg cggtgcggcc atcaaccgga tggtcgaggc gggcatcgag
     4081 aatgtcgaat tcatcgccgt caacaccgac gcccaggcgt tgcatcactc caaggcggcc
     4141 aagaaaatcc acatcggcaa agatgagacc cgcggtctgg gcgccggcgc cgacccgtcg
     4201 gtcggcaaac gatcggccga agagtcgcgt gaggaaatcc gccaggccct cgaaggcgcc
     4261 gacatggtat tcgtgaccat cggggccggt ggcggtaccg gcagcggcgc cggtccggtc
     4321 gtggctgagg cagccaagga acttgatatc ctggtggtcg gcttcgccac caagcccttc
     4381 gccttcgaag gcgagaagcg gcgcaagaac gccgaggtgg ccatcgagga gctgcgcgac
     4441 gccgtcgaca cgctcatcgt catcccgaac gaccggctgc tgcagaccat cgaccgtaac
     4501 acgccgctca tggaagcctt caaggtggcc gacgacgtgc tgcgtca
//
LOCUS       DASSFD010000041         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1555552, whole genome shotgun sequence.
ACCESSION   DASSFD010000041 DASSFD010000000
VERSION     DASSFD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555552"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFH39_00945"
     CDS             <1..313
                     /locus_tag="VFH39_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924381.1"
                     /translation="SEQEEHFRRRIRVYIEWVKSGVYRERFGASVVTVAFTTFAVDRR
                     LQQMRSWTNTELQSTQESAEVSGTFLFASLPPAPTSRQVWLEPCWYMASDNTAFSLLQ
                     S"
     gene            342..602
                     /locus_tag="VFH39_00950"
     CDS             342..602
                     /locus_tag="VFH39_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924382.1"
                     /translation="MTTLDEAIEKLAGHGGYGWLIDSKSAFWTVKQVVDALATEGRSV
                     SVSTVTGWFRGLPHTQGHVGKQGLIASRNDLIIMFADQMINK"
     gene            782..>2021
                     /locus_tag="VFH39_00955"
     CDS             782..>2021
                     /locus_tag="VFH39_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET6924383.1"
                     /translation="MARVAIYLRVSSIGQEENYSLPGQEEDCRNWCTANGHTVVKVYN
                     DGAQHSWTLNRPGLDQMFLDAKMDEFDILLIGRYDRLSRDIVQQNVVVYQLGMYKKQV
                     TSASQPLPEDRAMAELMRNLHGYAAQAELNAIRLRTTGGRKRRMRDGKLLVGTSQLYG
                     YRWANPDVKHGKDAYVIDPETAPVVQLIYDLLVANGRSLRFIAAELERRGIPTPGQVL
                     LDRGQLAKGRTVSSIWRLSTLRRILTNPAYIGKHTGWRHTTEEVMRKDKLTGELRAVR
                     YTRLREEDDPDRVAYDASVCPPIISDAVFNAAQIVLRRNKEEAARRMKDPKAALLRNG
                     FAICGHCGRKIQVKFHKQNSHYRYMCAAEQDLKSVHCPGGGWSMKAADLDAIVWGFVV
                     RAFENPDIIRAAFERYKEKSA"
BASE COUNT          443 a          564 c          623 g          391 t
ORIGIN      
        1 cagcgagcag gaagagcatt tcaggcggcg gatacgggta tatatcgagt gggtcaagag
       61 cggggtgtac cgggagcggt tcggggccag cgtggtcacg gtggcgttca cgacgttcgc
      121 ggtggatcgg cgactccagc agatgcgctc ctggacgaac accgaactgc agtcgacgca
      181 agagagcgcg gaggtgagcg gtaccttcct gtttgcttcc ctgccgcctg cgcccacgtc
      241 gcggcaggtg tggcttgaac cgtgttggta catggcgagt gacaacacgg cgttttcgtt
      301 gttgcagtcg taactcatag taggatggta tactcgcaac catgacaaca cttgatgaag
      361 caattgaaaa actcgctggg cacggtggct acggctggct gatcgacagc aaatcggcgt
      421 tctggacggt gaaacaggta gtcgatgcgt tggcgactga gggcagatca gtgagcgtct
      481 cgactgtcac cgggtggttc cgggggctac cgcatactca gggacacgtt ggcaagcagg
      541 ggctgatcgc ctcacggaac gatctcatca tcatgttcgc tgaccagatg ataaacaaat
      601 gacttgtttt gcccttgtat gtcgtgatag attgacaaac tcgtaatagc tccttatact
      661 ggtgacatga cggttcccca gcttccgcag gactatgcgc tcgacagagt gcttgcctgg
      721 ttattcagcg acccgaagct gggcctccat gtacgggata tgcaagtaag gagtgagggg
      781 catggcacgg gtggcaatct acctgcgggt atcaagcatc gggcaagaag aaaactatag
      841 cctgcctggg caggaagaag actgccgcaa ttggtgcaca gccaacggcc atacggttgt
      901 gaaggtctac aatgacggtg cgcagcacag ctggacactc aaccgtccgg gcctcgacca
      961 gatgtttctc gacgccaaga tggatgaatt cgatattctc ctcatcgggc gctatgaccg
     1021 cctctcacgg gatattgtcc agcagaacgt ggtcgtctac cagctcggga tgtataagaa
     1081 gcaggtaaca agcgccagcc agccgctgcc ggaagaccgc gccatggccg agctgatgcg
     1141 gaacctccat ggctacgcgg cgcaggccga actgaacgcc atccgcctgc gcacgaccgg
     1201 cggccgcaag cgccggatgc gcgacggcaa gctgctggtc ggcaccagcc agctctacgg
     1261 ctaccgctgg gccaatcccg acgtgaagca cggcaaagac gcctacgtta tcgaccctga
     1321 gactgcgccg gtcgtccagc ttatctacga tctccttgtc gccaacggaa ggtcgctgcg
     1381 gttcatcgca gctgaacttg agcgccgagg tattcccacg ccggggcaag tgctcttgga
     1441 tcgcggccag cttgctaaag gccgcaccgt tagttcgatc tggcggctat ctaccctgcg
     1501 gcgcattctg acaaatccgg cgtatatcgg caagcacacc ggctggcggc acaccactga
     1561 agaagtcatg cgtaaggaca aactaaccgg cgagctgcgg gcggtgcgct acacgcggct
     1621 gcgggaagaa gatgatcctg accgcgtagc gtacgatgcc tcagtatgcc ctccgattat
     1681 ctccgacgcc gttttcaacg ctgcccagat agtgctgcgg cgtaacaaag aagaagccgc
     1741 gcggcgcatg aaagacccga aagcggcgct cctgcgtaac ggcttcgcca tctgcggcca
     1801 ctgtgggcgc aaaattcagg tcaagttcca caagcagaac agccactacc gctacatgtg
     1861 cgcggccgag caggatttga agtccgttca ctgccctggc ggcggctggt caatgaaagc
     1921 tgccgatctt gatgcaattg tgtggggctt cgtggtgcgg gcctttgaaa accctgatat
     1981 tatccgcgca gcctttgagc ggtacaagga gaagtcggcg g
//
LOCUS       DASSFD010000042         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_535011, whole genome shotgun sequence.
ACCESSION   DASSFD010000042 DASSFD010000000
VERSION     DASSFD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535011"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VFH39_00960"
     CDS             complement(<1..1419)
                     /locus_tag="VFH39_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924384.1"
                     /translation="MLVRPEAIDYPPPETLPAVMDSIINEQVIAFHRTVVQAKDEATG
                     VLSLAADEAAMLLPEHMRRNQYLRLAAACRQTDTIGSVESQAIAAISKTVELARSTVL
                     IGRAVEAMHLNKYRPEIHGAAIEKASRHWDDEHRRRLSQKEAMTWDTMAEVASRSVST
                     AVGCKQIIEILQPRQPLLRRQLLRAVDVLDKLDRGQTIDPKADLSPICELTEEIFSDN
                     RYRQRQAVQSADEVLRDKIGTVIAERVKRYKQQGDERRSAAYNGLIIAMGGKVIEPSS
                     TPTTAKNTPQVELQLTAQAKHRHSRRSSRPPKPAVQPTATTEESAPKVIWNPRGVAAA
                     TALLATAAGIAAPASAASAPAASETAATVHYANPEATALPTAQKDLRTIVIKQPVAPG
                     AIEQQPTMPAKVAQPPETIQMPSSHDVPKSQNASVPETTVVQPTPGETSTTTVPTTDT
                     PTTPAQGPEQTDAPTNGNGDPQT"
     gene            complement(1458..2477)
                     /locus_tag="VFH39_00965"
     CDS             complement(1458..2477)
                     /locus_tag="VFH39_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924385.1"
                     /translation="MSIETATDFPTEQFGHPYTGSPHEAAFAGFTDEAAIQDLQLQAV
                     ADAGEVGETIAAYRYFEDSSTARKQEIDDFINCFAAASADYKETAEERASIKEAVVAV
                     LGTIRNLRDELAFVATELHTLAQRRKELHEEKQKEELALYTAQEGSKVLRQRIEPFEQ
                     VDRSLEEISEALAIPLPDSEPAEPYDERYTRSISQLRQDLAMQSGRAEDSTARLAEIA
                     EEIAQIDARRSEKVEVCQALRVKRSEVLAQHQELRNRIAILEAKRIESLNRIRELIRN
                     QSFGELMITGKTSVSGQPPLVPEAYAQASASPAAIPHLSPDSRQPTHAKVLRLPSAPQ
                     AGVGN"
BASE COUNT          418 a          699 c          799 g          696 t
ORIGIN      
        1 cgtttgcgga tcgccgttgc cgttcgttgg ggcgtccgtc tgttcggggc cttgggcagg
       61 ggtggtcggt gtatcggtag tcggcaccgt ggtggtcgaa gtttcgccgg gtgtcggctg
      121 taccacggtg gtttccggca ccgaagcatt ctggctcttc ggaacatcgt gcgaggacgg
      181 catctgaatg gtttccggcg gttgagcgac tttagcgggc atcgtcggtt gctgctcgat
      241 cgcgcccgga gcgaccggct gtttgatgac tatcgtccgc aagtcctttt gagcggtcgg
      301 cagggcggtc gcttccgggt tagcgtagtg taccgtggct gctgtttcgg acgcagcggg
      361 cgctgatgca gccgatgcag gcgcggcgat gccggctgcg gtggccagca gagccgtagc
      421 tgcggctaca cctctggggt tccaaatgac cttcggcgct gactcctctg tcgttgcggt
      481 gggctgtaca gcaggcttcg ggggccgact actgcgccgg gaatgccggt gctttgcttg
      541 tgctgttaat tgcaactcga cttgcggggt attctttgct gtcgttgggg ttgatgatgg
      601 ctcgataacc tttccgccca tggcaatgat cagcccgtta tacgccgcgc tacgacgttc
      661 atcgccctgc tgtttgtagc gcttgacgcg ctcggcaata accgtaccga tcttgtcgcg
      721 caggacttcg tcggctgact ggacggcttg gcgttgccgg tagcgattgt cgctgaatat
      781 ttcctcggtc agctcgcaga ttgggctgag atcagctttt gggtcgatgg tttggccgcg
      841 gtccaattta tcgagtacgt ccacagcccg caagagctga cgacggagga gtggctgcct
      901 gggctgcaat atttcaatga tttgcttgca gccaacagcg gtgctaaccg accggctagc
      961 tacttcggcc atggtatccc atgtcatggc ttctttttgg gacagccggc ggcgatgttc
     1021 gtcgtcccag tggcgtgatg ctttttcgat agcggcccca tggatttccg gtcggtattt
     1081 gttaagatgc atggcttcta cggcgcgacc gataaggaca gtgctgcgcg ccagttccac
     1141 agtcttactt atggcggcta tcgcctgact ttcgactgat ccaatcgtat cagtctgccg
     1201 gcaagcagca gcgagccgca ggtactgatt cctgcgcata tgttccggca gcagcatagc
     1261 cgcttcatcg gcggcaagac tcagcacgcc tgttgcttcg tccttggcct gtacaacggt
     1321 gcggtggaat gctatgacct gctcattaat gatgctgtcc atgacggcag gcagcgtttc
     1381 tggcggcgga taatcgatgg cttctggtcg tacgagcatc tcggtcattt ttggtcccct
     1441 ccaacgattt ttagtttcta gttaccgact ccggcctgcg gcgcactcgg cagtcgtaaa
     1501 acttttgcgt gagtcggctg acgagagtcc ggcgataaat gagggattgc ggccggactc
     1561 gcggacgcct gagcgtaggc ctccggtaca agcggcggtt ggccagaaac actcgtcttg
     1621 ccggtgatca tcagttcacc aaagctctga ttgcggatca attcccgtat ccgattcagg
     1681 ctctcgatgc gcttggcctc cagaatggcg attcggttcc gaagttcttg gtgttgagcc
     1741 aggacctcgc tccgcttaac cctcagagcc tggcagactt ctaccttttc ggagcgccgg
     1801 gcgtctatct gggcgatttc ttcggcaatc tccgcaaggc gggcagttga gtcttccgcg
     1861 cgaccgcttt gcatagccag gtcctggcgc agctgcgata tggaacgggt gtatctctca
     1921 tcataaggtt cggctggttc tgagtccgga agcgggatgg ccagcgcctc cgatatttcc
     1981 tctagagatc tatctacttg ttcaaaaggt tcaatgcgtt gccgcaaaac cttgctgccc
     2041 tcttgggcgg tgtagagggc cagttcttct ttttgctttt cttcgtgcag ctctttgcgc
     2101 cgttgggcta aggtgtgtag ttcggtagcc acgaatgcca gctcatcgcg taaattccgg
     2161 attgttccta ataccgctac cactgcttct ttgatgctgg cccgttcttc tgcagtctct
     2221 ttgtaatctg cgctggcggc ggcaaaacaa ttgatgaaat catcgatttc ctgcttccgc
     2281 gctgtgctgg agtcttcgaa gtaccggtac gcggcgatgg tctcacctac ttctcctgca
     2341 tcggcgacgg cctgaagctg cagatcttga atagctgctt catcagtaaa gccagcaaac
     2401 gccgcttcgt gtggtgatcc ggtgtatggg tggccgaact gctcggttgg aaagtcagtg
     2461 gctgtttcga tgctcatttt ttggtacctc ctgtgctgct ggttgtgctc gtgggtgttg
     2521 gggcaacaga tgctgctggc tggcgggacg gctgttggct gattttcgga tgctcgagtc
     2581 gcttctgctt aatcttaagc gtcgcattga gg
//
LOCUS       DASSFD010000043         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_527365, whole genome shotgun sequence.
ACCESSION   DASSFD010000043 DASSFD010000000
VERSION     DASSFD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527365"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..404
                     /locus_tag="VFH39_00970"
     CDS             87..404
                     /locus_tag="VFH39_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924386.1"
                     /translation="MIARIAAAFINFFLALTEIFLGVRFLLRFFAANPANSFVHWVYN
                     SSDILLQPFRGMFGTTVVGNNHVVEFNTLFAMAIYAVAALILLWLVSFFDPVRYRTAV
                     VRK"
     gene            complement(412..1932)
                     /locus_tag="VFH39_00975"
     CDS             complement(412..1932)
                     /locus_tag="VFH39_00975"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6924387.1"
                     /translation="MNGTNEKIGSLIAQVRQERGLTQAAFAKLLNTSQSAVNRMEHGK
                     QNLSMETLGRISDVLNKQLIAVSSGGVNLRIEGGHELKGNITLKTSKNAAVGLLCASL
                     LNYGVTIFKSFPRIEEVYRIIEVLESLGVRVRWLPGNDLEIRRPEQLKLDNLNSVAAR
                     KTRSVLMLIGSIMHDCEEFKIPYAGGCKLGTRTVEPHLFALEEFGVSILATKGNYNVT
                     VNKKPAGRVTLYEPGDTVSENALMAAARTPEATFIEFASANYSVQEVCFFLQKLGVEI
                     EGIGTTKLTVRGLPFIKKNVTYAPSEDPIEAMFFTSAAVVTNSSITIQRVPIEFMSVE
                     LLKLKKMGLEYTESERYKAANGKTDLVDLSIEKHNGSLHALADKIHSLPFPGLNADNL
                     PYFVPIACVARGRTLIHDWMYENRAIYYTELTKIGANVELADPHRMYVNGPTKFTPAD
                     VVCPPALRPASLLLIGMLAANGISMLRNIYTIQRGYEDLAERLNSLGANIAVLNDM"
BASE COUNT          396 a          555 c          618 g          536 t
ORIGIN      
        1 cgctcctgtc gcttcggtag ttagattctt tacaaacaac cagaaaaaag agtgctatta
       61 ctagagtatg taataaggag ttgcttatga tcgctcgaat cgccgccgca ttcatcaact
      121 tttttctggc gctgacagag attttcctgg gcgtccggtt tctgctgcgc tttttcgccg
      181 ccaatccggc caactcgttc gtgcactggg tgtataactc cagcgacatc ctgctgcagc
      241 cgttccgcgg catgttcggc accacggtag tcggcaataa ccatgtcgtc gagttcaata
      301 cgctgttcgc catggctatc tatgccgtcg cggcgctgat actgttgtgg ctggtttcgt
      361 tcttcgaccc ggtccgctac cgtactgccg ttgtccggaa atagccgaca gctacatatc
      421 gttcaatacg gcgatgttgg cgcccaggct attgaggcgc tcggccaggt cttcgtagcc
      481 gcgctggatg gtgtagatgt tgcgcagcat acttattccg ttggccgcca gcatgcctat
      541 gagcaggagg ctggcaggcc ggagcgccgg cgggcagacc acgtcggcgg gcgtgaattt
      601 tgtaggtccg ttgacataca tacggtgcgg gtcggccagc tcgacgttgg cgccgatttt
      661 ggtaagctcc gtgtagtaga tggcgcggtt ttcgtacatc cagtcgtgaa tgagcgtgcg
      721 gccgcgggcc acgcaggcta tggggacgaa atagggcaga ttatcggcgt tcaggcccgg
      781 gaagggcagg gaatgtatct tgtcggccag agcgtgcagg ctgccattgt gcttttctat
      841 gctcaggtct accagatccg tcttgccgtt agcggccttg tagcgctcgc tctcggtata
      901 ctccaggccc attttcttga gcttcagcag ctctaccgac atgaactcta taggtacgcg
      961 ctggatggtt atagaagagt tggtaaccac ggcggcggaa gtgaagaaca tggcttctat
     1021 gggatcttcg ctcggcgcgt aggttacgtt cttctttatg aacggcaggc cccgtacggt
     1081 caacttggtg gtgcctatgc cttctatctc cactcccagc ttctgcagga agaagcagac
     1141 ttcctgtacg gagtagttgg cgctggcgaa ttctatgaat gttgcttcgg gggtgcgggc
     1201 ggcggccatt agggcgtttt cgctcacggt atcgcccggt tcgtacaggg tcacgcggcc
     1261 ggccggtttt ttgttaacgg ttacgttgta gttgcccttg gtggccaata tgctgacacc
     1321 gaattcctca agtgcgaaca gatgcggctc cacggtgcgg gtgcccagct tgcagccgcc
     1381 ggcgtacggg atcttgaact cctcgcagtc atgcatgatg gagccaatga gcataaggac
     1441 gctgcgcgtt ttgcgggcgg cgaccgaatt gaggttgtcc agtttgagct gctcggggcg
     1501 gcggatttcc aggtcgttgc cgggcagcca ccgtacccgt acaccgagac tttcgagcac
     1561 ttctataatc cggtacacct cttcgatacg ggggaatgac ttgaagatgg tgaccccgta
     1621 gttcagcagg ctggcacaca gcagaccgac cgcggcgttc ttgctggttt tgagcgtgat
     1681 gttgcctttg agttcgtggc cgccctcgat cctgaggttc acaccgccgc tgctgacggc
     1741 gataagctgc ttgttgagaa cgtcgcttat acgccccagt gtttccatgc tcaggttctg
     1801 cttgccatgc tccatccggt tgaccgccga ctggctggtg ttgagcagtt tggcaaaggc
     1861 ggcctgcgtc agtccgcgct cctggcgtac ctgggctatc agcgaaccta tcttttcgtt
     1921 ggtgccattc atagcagaga gactatatca tatatgatat tgtagttcca acggcagaac
     1981 ggtgcataaa ccccgtagaa attgctacaa tacccacatg gtttagcttt cttacccact
     2041 gacttatact gaatgtaacg aacggagaaa gctgatatga ctgatgaaga tgttgcgcgg
     2101 ctgat
//
LOCUS       DASSFD010000044         4437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1299006, whole genome shotgun sequence.
ACCESSION   DASSFD010000044 DASSFD010000000
VERSION     DASSFD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299006"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFH39_00980"
     CDS             <1..551
                     /locus_tag="VFH39_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924388.1"
                     /translation="EVNCRALSGPSAIIARMRWIGISGSWGASSSELVNDVQLSVRNV
                     MEAGNGIVTGGALGVDQLATTEALRHDPEAKRIKIIIPSSLEIYAAHYRNRASEDVIT
                     IEQAEALIGLLTIIQNKQSLTEMTYTSLNEKSYYARNTEVLNQSDELLVFQVNGSAGT
                     QDAIDKARELGMPVQVRQYEIR"
     gene            737..1648
                     /locus_tag="VFH39_00985"
     CDS             737..1648
                     /locus_tag="VFH39_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924389.1"
                     /translation="MKFQSGFTTVEALLVLFFVVFIGYIAWFVYQRDQQLQQTATTQA
                     PNAVVAQLKQQVTKKYSYASADTSQWVAQMGSGAGVTAAGYDYAVSTQSFPSIYFGLK
                     ADYNPAKAKHKFPGGVNGEIQNVLKADGYTIVANKYTVTGTGSAKPTYTTYVNDGTTC
                     LDIYDANQNVVTVSCYTQDNVVHAFARVKPFVTGYLAAHPELKPQNVVFGPVTVKDQS
                     NGGVITASKSPQYGISEAVVTVNGQKKLVLYYNTQWQYVTQANDEYGFPCSDYAANPE
                     LQTVMQGQVCLGDKGQMHFGTGTSATQ"
     gene            1836..2399
                     /locus_tag="VFH39_00990"
     CDS             1836..2399
                     /locus_tag="VFH39_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET6924390.1"
                     /translation="MNEMSHVTGGLVLRGVVATLFGVAAVFWPGITLVTLIYLFSAFV
                     LISGIVNLVFGLTKLFRGVTSWYAVVLNLVLGVLEVGVGVYLLRHINVGFTTLVLLIG
                     FLLIVRGLFEVVEGLLGTRTAGHRVVLVVVGLLALAAGIIMLFQPVSGGVAFVWILGL
                     YGIIAGPLMIAAAVELNRLSAAPARSR"
     gene            2503..3942
                     /locus_tag="VFH39_00995"
     CDS             2503..3942
                     /locus_tag="VFH39_00995"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6924391.1"
                     /translation="MSILQSLTRHRLKFGLAVAMVVLALGIGWAVWGITDAPPPSAAA
                     GIHTIKHVVIIMQENRSFDSYFGTYPGADGIPMRSGVPTVCSPDPRTGHCQRPYHDTQ
                     TVNTGGPHGSRNSQADINGGKMDGFVAQSEGSSKNCDNQPDCAVAKPSDVMGYHTAAE
                     IPNYWSYARNFVLQDHMFESASSWSVPEHLYMVSEWSAKCLVAGQPMSCTNDLRATDI
                     AEPAVNKGGRNKAAASRPAPDYAWTDLTYLLYQHHVSWGYYVFNGTEPDCEDDSAMTC
                     TPVRQNAHTAGIWNPLPYFDTVRQDGQLGNVQSLTNFYTAARKGNLPAVSWIAPAGAV
                     SEHPPASVTAGQAYVTGLINSIMQGPDWNSTAIFLSWDDWGGFYDHVVPPQVDQNGYG
                     IRVPGLVISPYAKQGYIDHQTLSHDAYIKFIEDDFLGGQRLDPANDGRPDPRPDVRES
                     NPALGNVAKDFDFNQQPRRPIVLSGGAIY"
     gene            3981..>4437
                     /locus_tag="VFH39_01000"
     CDS             3981..>4437
                     /locus_tag="VFH39_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6924392.1"
                     /translation="MTLHRAEAKPDWERVAKRRRNNWQQLAARTHGWLTPGNVLTFLG
                     AVLVVAGLIMLHSKLYLLSFLLIIVGRLCDLADGWAAHRTGTKSPLGEKLDAGMDKLL
                     GLVALIALVAMHYFPLWAALVIIAVNLAISLPSALSLFEGKTIHPSRAGK"
BASE COUNT         1007 a         1179 c         1261 g          990 t
ORIGIN      
        1 aagaagttaa ttgccgagcg ttgtctggtc cttctgctat aatcgcccgc atgcgatgga
       61 ttggtatttc tggctcttgg ggtgcctcaa gctccgagct tgtgaacgat gtacagttat
      121 cagtaagaaa tgttatggaa gctggcaatg ggattgtaac tgggggtgct ctgggcgtgg
      181 atcagctagc cactacggaa gcattaaggc atgaccctga agcaaaacgg attaaaatta
      241 tcattccttc cagccttgaa atttacgctg ctcattatcg caatagagct agtgaagacg
      301 tgattacaat tgaacaagct gaggcattga taggcctgct aactatcatt caaaacaaac
      361 agtcactgac cgagatgacc tacactagtc tcaacgagaa gagttactat gctcgtaata
      421 cagaggtgct taatcaatct gatgagcttc tcgtatttca agtaaatggt agcgctggca
      481 cacaagacgc gattgataag gcgcgtgagc taggaatgcc cgttcaggtg agacagtatg
      541 agattagata gcaggttcta gcctgcggcg atagagtaac ctgcttgcgt taactctcta
      601 gataactgta atcaacaaca taactctgga aggattgggc tctaaccgta tcaactcggc
      661 aatcaaaata cgatggccta atggtgcgca ttgtcaaagc accacggctg gcataagctt
      721 ataggatatg aagaaaatga aattccaatc tggttttact acggttgagg cgctgctggt
      781 attgttcttc gtggtgttca tcggctacat cgcctggttc gtgtatcagc gggaccagca
      841 attgcagcaa acggctacga cccaggcgcc gaatgcagtg gtggcccagc tgaagcagca
      901 ggtaaccaaa aagtacagtt atgcgtcggc cgataccagc cagtgggtag cgcagatggg
      961 ctccggggcc ggggtgacgg cagctggcta tgattacgcg gtgagcacgc agagttttcc
     1021 atcgatctat ttcggactga aagccgacta caatccggct aaagccaaac acaagtttcc
     1081 gggtggcgtg aatggcgaaa tacagaacgt cttaaaggcg gatggctaca cgatagttgc
     1141 taataagtac accgtcacag gcaccggttc ggcaaaacct acctatacca cctatgtaaa
     1201 cgacggtacg acgtgcctgg atatatatga tgcaaatcag aacgtcgtga cggtcagctg
     1261 ctatacccaa gataatgtgg tgcacgcctt cgcccgcgtc aaaccgttcg ttaccggcta
     1321 tctggccgcg caccccgaac tgaaaccaca gaatgtcgtg ttcggcccgg taactgtcaa
     1381 ggatcaatca aacggtggag tgattacggc cagcaagtcg ccacagtacg gtataagcga
     1441 ggccgtggta accgtaaacg gccagaaaaa gctagtgctg tattacaaca cacagtggca
     1501 gtatgtcacc caggccaacg acgagtacgg ctttccgtgc agcgactacg ctgccaaccc
     1561 cgagctgcag actgtcatgc agggtcaggt atgtttaggc gacaagggcc agatgcactt
     1621 cggtactggc actagcgcga cccaatagta tctttctgat ccgttaagct tgcttttctt
     1681 tagtgcgtcg cttgtgctta ctgcaaagcg gctaactcta tcgctcaata tttcaatctt
     1741 gaccacctca agagtagatg gccgaattat gtgattaata ttacagacac cccagcgcag
     1801 cgggcggata ttcaggttat aaccaaggag ggtacatgaa cgagatgtca cacgtaacgg
     1861 gtggactggt tcttcgaggc gtggtggcga cgctgtttgg ggttgcggcg gtcttttggc
     1921 cgggcataac attggtaacg ctcatttatc tgtttagcgc cttcgtactt atcagcggta
     1981 tagtcaacct tgtatttggt ctgaccaagc tgttccgcgg cgtcaccagc tggtatgcgg
     2041 tggtgttgaa cctggtatta ggtgtgctgg aggtcggcgt cggcgtctat ctgctgcgcc
     2101 acatcaacgt cggtttcaca acactggtgc tgttgatcgg tttcctgctg atcgtacgcg
     2161 gactgttcga agtagtggaa ggactgctgg ggacacgtac ggcgggtcat cgggtcgttc
     2221 tggtagtggt tggcctgctg gctctggcgg ccggtatcat catgctgttc cagccggtat
     2281 cgggcggcgt ggcattcgtc tggatcttag gcctctacgg catcattgcc ggaccgctca
     2341 tgattgcggc ggcggtggag ctgaaccgat tatcggctgc ccccgcgcgg tctcgataat
     2401 acgggcatcg taccataacc gggccgaact gatcggcccg gttttatctt ggtatatgat
     2461 gtctcattac ttcgtagtgc ataagaagca tacaatagga gcatgtctat attgcaatca
     2521 ctcacgcgcc accggttgaa gttcggtctt gccgtggcga tggtagtatt ggcgctgggc
     2581 atcggatggg ccgtgtgggg tatcacggac gccccgccgc cgagtgcggc cgccggtatt
     2641 cacaccatca agcacgtcgt cataatcatg caggagaacc gatctttcga cagctatttc
     2701 ggtacctatc caggcgccga tgggatacct atgcgtagcg gcgtaccgac cgtctgttct
     2761 ccggacccgc gaaccggtca ctgccagcgg ccttaccacg acacgcaaac ggtcaatacc
     2821 ggcggtccgc acggttcccg caactcccaa gccgacatca acggtggcaa gatggacggt
     2881 ttcgtggcgc agtcggaagg ttccagtaaa aactgcgata accagccgga ctgtgctgtc
     2941 gccaagccaa gcgacgtcat ggggtaccat acggcggccg agataccgaa ctactggagc
     3001 tacgcccgca acttcgtcct gcaggatcat atgttcgagt cggcgtcatc gtggagcgtc
     3061 ccggagcact tgtacatggt atccgaatgg tcggctaaat gcttggtggc cggccagccg
     3121 atgagctgca cgaacgatct gcgggctacc gacatcgccg aacctgccgt caacaagggt
     3181 gggaggaaca aggcagcagc atcccggccg gcaccggatt atgcgtggac agacctcacc
     3241 tatctgctgt atcagcatca tgtgtcgtgg ggctattacg tcttcaatgg caccgaaccc
     3301 gattgcgagg acgattccgc catgacctgt acgccggtgc ggcagaatgc ccataccgcc
     3361 ggcatctgga atccactgcc ttacttcgat accgtccgcc aggacggtca gctcggcaac
     3421 gtgcaatcgc tgaccaattt ctacacggca gcacgcaaag gcaatctgcc ggccgtgtcc
     3481 tggattgccc cagcaggggc ggtcagcgag cacccgccgg catcggtcac agcgggccag
     3541 gcatacgtaa cagggcttat caacagcatc atgcaaggtc ccgactggaa cagcacggca
     3601 atcttcttgt cgtgggacga ctggggcggc ttttacgacc acgtggtgcc gccgcaggtg
     3661 gatcagaacg gctacggcat ccgggtgcct ggcctggtca taagccccta cgccaagcag
     3721 ggctatatcg atcaccagac acttagccac gatgcctaca tcaaattcat agaggacgat
     3781 ttcttgggtg gccagcggct tgacccggcg aacgacggcc gccccgaccc ccgcccggat
     3841 gtccgggaaa gtaatccggc tctcggtaac gtggcaaaag atttcgattt caaccagcag
     3901 ccgcgacgcc ccatcgtgct gtccggcggc gcgatttact gaccggtcgg tcagcctggt
     3961 ggttatgcta tagtgcagct atgacactgc atcgggcgga agcgaaaccg gactgggagc
     4021 gggtagcgaa gcgccgccga aacaactggc aacagctggc ggcccgtacc cacggctggc
     4081 tgacaccggg caacgttctg acattcttgg gggcggttct ggtcgtggcc ggactgatca
     4141 tgcttcattc aaaactttac ttgttaagct ttttgctgat tatcgtcggg cgcctctgcg
     4201 acctggcaga cggttgggct gcgcaccgga ccggtaccaa aagcccgttg ggcgaaaagc
     4261 tggatgccgg catggacaaa ctgctcggtc tggtagcgct tattgctctg gtcgccatgc
     4321 actactttcc attgtgggca gcgctggtca tcattgcggt gaacctggcc atcagcctgc
     4381 cttctgccct gagccttttt gagggtaaaa cgatacaccc gtcgcgcgct ggcaagc
//
LOCUS       DASSFD010000045         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1351007, whole genome shotgun sequence.
ACCESSION   DASSFD010000045 DASSFD010000000
VERSION     DASSFD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351007"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFH39_01005"
     CDS             <1..875
                     /locus_tag="VFH39_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HET6924393.1"
                     /translation="LMMGGAQAQMQSQGGSLLAPALSPSEEADLDAALIETYAKAGIT
                     NDPLTHTAQPPTIIDLYDTLLHMGGNGPQLAQRLRKYTSGTFAGIFSQQSNIDINNPL
                     VVFNIRDLEDELRPVAMYIVLNYIWNKTKTDQKRRLLIVDEAWQLMKYEDSANFMFSL
                     AKRARKYNLGITTITQDVEDFMGSRMGRAIVANASMQILLKQSPSAVDVLADVFKLTA
                     EERKRLSQFPVGQGLFFAGQNHVHIQVVASPTEQSLITTNPAELQNMAQGQGGEFNQP
                     QGTIDLNNRLAPGV"
     gene            917..>3926
                     /locus_tag="VFH39_01010"
     CDS             917..>3926
                     /locus_tag="VFH39_01010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6924394.1"
                     /translation="MANSANQLFKDDEDERVTGRPSDDQLRHITGINHDDEAGMEDRA
                     QNGSAAEAGDSLDRQERNPDGGFFRSEQPENKQADKFAGRPELQDGEQGINPPGGLFK
                     REGEAGAQGLRNRLAGINLKSRKSIAGIGISLLLGGGGFFGIGILSGPAMLIQVSHVL
                     QKTMDKGQSDTSNRSGHLFRYFRALRKGDITETRVGRLGSSVFGNTMKQLKDIGIEFD
                     RGISGKPTRTYIDTKNEKLREQFPETEKMSPSELTDFYAKQLGLTESDRIKIDTAPGS
                     ETEGRITIDQTGMNMDASRALTKSTIRLLNDGIIGEGLKVRYVTKMFNEPNMFHQLNR
                     KTAQLENKVSDNLKNKVGEDEEAKRKASLTDTAEATPSLNEPSGDEAEKAIEDKSSGL
                     KSTVLKAMTYQAGACFAQDIAKEIPALDRAKVVLPAIAQATSLISIGEQIENGGSDVT
                     NEQLAAVTQGLFDQKGNSVLAGQAMKALEGDGTVDKSNDYYDISPDQKQAFKSHDTAN
                     MIYDWASGITGGKVISKFTCNSIVLWTEAGVALVFSAASAIGSGGTLTPAIVGFWAFK
                     AGLQVAATAVFMHYLQSWILDSTTAKALAKSAFSGPLGGSLLAYGARAASVAVGLASG
                     GIDLGAKALTLVDSQQQQQQEQQWRNESFASRLFNVYDYHSLAGQAIQNVSPSASENA
                     SSFASSVLNVGSLLSHAFASLIPKTSADGKHAYDWGFSQVGLPDFMLNDPRLADPYDN
                     AGQIVSLLNANNSESSRLIDKAKHCFGDTISKDNPDGVWDVTTGEGVDPQSDDYQSAN
                     CNPFNGSIDNDVNWARMIMFVFDTQTMKAVACYQGDETSCSDLGAGGSSTDSGTPPPT
                     TPGTYKNPLRGIKNIVPERIDGGVDYNGDAGPIYAVGDGVVKLVETAPSSGWPGQPGA
                     YIMYQLTDGKAQNKYIYMAEDCTPTVKTGQKVTADTVICNYRYKGTAMEMGWSDGTNN
                     YVTDDYRSHGGGFYASNSGQDFSNFLFSLGA"
BASE COUNT         1037 a         1050 c         1081 g          758 t
ORIGIN      
        1 gcctgatgat gggcggggcg caggcacaga tgcagtctca gggcggctcg ctgctggccc
       61 cggcactcag cccaagtgaa gaagccgacc tggacgccgc actgattgag acatatgcca
      121 aagctggcat caccaacgac ccgctgaccc ataccgccca gccgccgacc atcatcgacc
      181 tgtacgacac cttgttgcac atgggtggca acggcccgca gctggcccag cggctgcgca
      241 agtacacctc cggcaccttt gcgggtatct tcagccagca gagcaacatc gacatcaaca
      301 acccgctggt ggtgttcaac atccgtgacc tggaggacga gctgcgaccg gtggccatgt
      361 acatcgtgct gaattacatc tggaacaaga ccaagaccga ccagaagcgc cgactgctga
      421 tcgtcgacga ggcctggcag ctgatgaaat acgaagacag cgccaacttc atgttctcct
      481 tagccaagcg ggcgcgcaaa tataacctcg gcatcaccac catcacccag gacgtcgaag
      541 atttcatggg gtcgcgcatg ggccgggcca tcgtcgccaa cgccagcatg cagatactgc
      601 tcaagcagtc gccgagcgcc gtcgatgtgc tggccgacgt cttcaaactg accgccgagg
      661 agcgcaagcg cctcagtcag ttcccggtgg ggcagggact gttctttgcc ggccagaacc
      721 acgtgcatat ccaggtggtg gccagcccga ccgaacaaag ccttatcacc accaatcccg
      781 ccgaattaca gaacatggcc cagggccaag gtggtgaatt caaccagccc cagggtacca
      841 tcgatttgaa taacagattg gcaccgggag tataatagac acgtaattcc acacttggcg
      901 tgaggccaaa accaacatgg ctaacagtgc aaaccagcta ttcaaggatg atgaagacga
      961 gcgagtaact ggccgtccga gcgacgatca gcttcgccac atcaccggta taaaccacga
     1021 cgatgaggcc ggcatggaag accgtgccca aaatggatct gccgccgaag ccggggactc
     1081 gcttgaccgg caagagagaa accccgatgg cggcttcttc cgctctgagc agcccgaaaa
     1141 caagcaagcg gataaatttg ccggacggcc agaactgcag gacggcgaac agggcataaa
     1201 cccacccgga ggcttattta agcgggaggg cgaggctggg gcgcaaggac tgcggaatcg
     1261 ccttgcgggc atcaatctga agagccgcaa aagcatcgcc ggcatcggta tcagccttct
     1321 gctcggcgga ggcggttttt ttgggatagg tatcttaagc ggcccggcaa tgctcattca
     1381 ggtgtcgcac gtcttacaga aaaccatgga caagggccaa tccgatacgt ccaataggag
     1441 cggccacctg ttccgatatt tccgtgcctt acgcaaagga gatattaccg agacaagggt
     1501 aggtaggctg ggcagttcgg tattcggcaa cactatgaaa caactgaagg atatcggtat
     1561 tgagttcgat cgaggtatca gtggcaaacc gactaggacc tacattgata cgaagaacga
     1621 aaaattacgt gaacagttcc cggaaaccga aaagatgtcg cccagcgaac tgactgactt
     1681 ctatgcaaaa cagctcggct taacagagag cgaccggatc aaaatcgata ctgcaccagg
     1741 cagtgagacc gaggggcgga ttaccatcga tcaaaccggt atgaatatgg acgcgtcccg
     1801 tgccttaaca aagagcacca ttagactgct taatgatggg atcattggtg aaggcttaaa
     1861 ggtcagatac gtcactaaga tgttcaacga gccgaatatg ttccaccaac tgaacagaaa
     1921 gacggcccag ctggaaaata aggtgtccga taacctcaag aacaaagtcg gagaggatga
     1981 ggaggctaag cgcaaagctt cgctgaccga tacagccgag gcaaccccaa gccttaacga
     2041 gccaagcgga gatgaagctg aaaaggctat cgaggataag tcgtcgggtt taaaaagtac
     2101 tgttctgaag gcgatgacct atcaggccgg tgcctgtttt gcgcaagaca tcgccaaaga
     2161 aataccagcc ctcgatcggg cgaaggttgt attgcccgct attgcgcagg ctaccagctt
     2221 aatttctatt ggtgaacaga ttgaaaatgg cggctcggac gtaaccaatg aacagctcgc
     2281 agcggtgaca caaggactgt ttgaccaaaa aggtaactcg gtcctggccg ggcaagctat
     2341 gaaggccctg gaaggtgacg gcaccgttga taaatccaat gactactacg atatttcacc
     2401 tgatcaaaaa caagcattca aaagtcacga tacggcaaac atgatatatg actgggcaag
     2461 cgggattact ggcggcaagg tcataagcaa gtttacctgt aatagtatcg tgctctggac
     2521 agaggcaggt gtggctttgg ttttctctgc cgcctccgct atcggcagtg gcggcacact
     2581 tactccggct atcgtgggct tttgggcgtt taaggctggc ctgcaggttg ctgctactgc
     2641 cgtgttcatg cattacctgc agagctggat ccttgacagc actaccgcaa aagcactggc
     2701 caaaagcgca tttagcggtc ctctgggtgg ttcattactg gcctatgggg cacgcgctgc
     2761 gtcagtagca gtagggcttg cgtcaggtgg tatagattta ggagccaagg ccctcacgct
     2821 tgtcgatagc cagcaacagc agcagcaaga acagcaatgg cggaatgaaa gttttgccag
     2881 taggctattt aacgtctacg attatcattc gcttgccggc caggcaatac agaacgtcag
     2941 tcccagtgca tcagaaaacg catcaagttt cgccagcagc gttttgaacg tcggctcgct
     3001 cttaagtcac gctttcgcat cactaattcc caaaacttca gccgacggca aacatgctta
     3061 cgactggggt ttttctcagg taggcttgcc cgacttcatg ttaaatgacc cgcggctcgc
     3121 cgatccctac gacaacgcag gacagatcgt aagtctgctg aacgctaata acagcgagtc
     3181 gtctcggctc atagacaagg ctaagcattg ttttggcgac acaatcagca aggacaatcc
     3241 ggacggggta tgggatgtga ccacagggga aggggtcgac ccgcaaagcg acgactacca
     3301 gtccgccaac tgcaatccct ttaacggaag catagacaac gacgttaatt gggcacgtat
     3361 gatcatgttc gtattcgaca cccagaccat gaaagcggtg gcctgctacc aaggtgacga
     3421 gacgtcgtgt tctgatctcg gcgcgggtgg tagcagcacg gatagtggca ctccgcctcc
     3481 cactacgccg ggtacctaca agaatccgct gcggggcatc aagaacatcg taccggaacg
     3541 cattgatggt ggagttgact acaacggtga cgccgggccg atatatgcgg ttggcgacgg
     3601 ggtagtaaaa ctagtagaga cagcaccctc atctggctgg cctgggcagc ctggcgctta
     3661 cataatgtat caactgactg acggtaaggc ccagaataaa tacatctaca tggccgagga
     3721 ctgcacaccg acggtaaaaa ccggccagaa agttacagcc gatacggtga tatgcaatta
     3781 ccgctataag ggaacggcca tggaaatggg ctggagcgat ggcactaata attacgtcac
     3841 cgacgactat cggtctcatg gcggcggttt ctacgcgtcg aactctggac aggatttctc
     3901 taacttcctc ttcagtctcg gcgcac
//
LOCUS       DASSFD010000046         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1435320, whole genome shotgun sequence.
ACCESSION   DASSFD010000046 DASSFD010000000
VERSION     DASSFD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435320"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..587
                     /locus_tag="VFH39_01015"
     CDS             33..587
                     /locus_tag="VFH39_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET6924395.1"
                     /translation="MRKLIMWNVVTVDGYFEGKQAWDLEFHQAAWGADMEEFIMEQLK
                     SVDMLVFGRKTYQGMADYWTNAEREPETKHMNALPKIVCSTTLQKADWNNTTIVKDAV
                     AAIAKLKQAGEGNMFVFGSGELCASLMKAGLFDEYRLCIAPVFLGTGRRLFGEGIPYQ
                     KLRMLEERQLQSGGVIVKYATDSE"
     gene            589..1167
                     /locus_tag="VFH39_01020"
     CDS             589..1167
                     /locus_tag="VFH39_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET6924396.1"
                     /translation="MGKLIYLMTTTLDGFAADDAGKFDWARPTEEVHAFVNDQLRNVA
                     TILMGSKLYETMKVWEDIPTEGVGGFMDGPSQALNDYAKIWHGANKIVYSTSLTDVAI
                     ANTTIERKFDVGAVRKLVAESARDVDIGGPHLAAEAIKANIVDEYHHIIAPVLIGSGL
                     RWLPEGAHSQLQLVSTRTFENGFVHLHYRKKI"
     gene            complement(1241..1465)
                     /locus_tag="VFH39_01025"
     CDS             complement(1241..1465)
                     /locus_tag="VFH39_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924397.1"
                     /translation="MAKLIVILIPLVLAAFWLWMFHDMLHNDDIPSTDPPGLRWPPQA
                     KNHWVVYFIVLNIFTAGYYYFTEYGKGSER"
     gene            complement(1469..1786)
                     /locus_tag="VFH39_01030"
     CDS             complement(1469..1786)
                     /locus_tag="VFH39_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET6924398.1"
                     /translation="MTAKILDDGVAHKLPADMRQALLASPKALAAWNSITPLARNEWI
                     CWTITVKTAATRQNHVKRTVSELTEGMRRPCCWIGCIHRPDKAISPSVQWVLNKRRTG
                     ASK"
     gene            complement(1886..>2747)
                     /locus_tag="VFH39_01035"
     CDS             complement(1886..>2747)
                     /locus_tag="VFH39_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924399.1"
                     /translation="LWLLVPEAKTSSERLQMAGRPVTVDSLKEIVERADVKGAARRAN
                     DTLSGPAARVRDVIDTAFRLLVKIVGIGLTLFGLFMLALLAFGSIYLSVHGNVIYDNL
                     FPIGFQEHLLVYMAVFVAALMTLFIILFGMAIFRRKWPIRTWLTGVLVGLTVIGLVSG
                     SALAANVVPRVRDRYNAHFNTLVKNVAPFTKLNVVGPQAAVSLQTSDKYYVAVKYYDK
                     SDPSKIKVSVKNGTLTIDNTQYQWDRHCSSLCLPNHFDAQVTVYSPNPPAVSLPDMPP
                     KPVVVGPLYP"
BASE COUNT          618 a          689 c          858 g          582 t
ORIGIN      
        1 aaattagcag ctgacagtgc tacgataagg ttatgagaaa gctcattatg tggaacgtgg
       61 ttacggtgga tgggtacttc gagggcaagc aggcttggga tttggaattt caccaggcgg
      121 catggggtgc ggacatggaa gaattcatta tggagcagct aaagtcggtg gatatgctgg
      181 tttttggtcg taagacatac caagggatgg ccgattactg gacgaacgcc gagcgcgagc
      241 cggagactaa gcacatgaac gcgctgccga aaatcgtctg ttcgactacg cttcaaaagg
      301 cagactggaa caatacgacc atcgtaaaag acgcggtggc cgcaatagcg aagctaaagc
      361 aagcaggcga gggcaatatg ttcgtgtttg gcagcggcga gctgtgcgca tcgctcatga
      421 aagccggtct gttcgacgaa tatcgcttgt gtatagcgcc ggtatttttg ggcaccggca
      481 ggcggctatt tggcgaaggc ataccctacc aaaagctacg aatgctcgag gagcgtcaac
      541 tgcaatccgg cggtgtcata gtaaagtacg ctacggattc agaatagtat gggtaagctc
      601 atttacctca tgacgacgac gctcgacggc tttgcggccg atgacgccgg taagtttgac
      661 tgggcaagac cgaccgaaga ggtccatgcc ttcgtgaacg accaactgcg gaacgtggcg
      721 acgatactga tggggagcaa gctgtacgag acaatgaagg tctgggaaga tattccgact
      781 gagggagtcg gtgggtttat ggacgggcca agtcaggcgc tgaatgacta tgccaaaatc
      841 tggcatggcg cgaacaagat agtctattcc acttcgctta ccgatgtcgc catagcgaat
      901 acgacgattg agcggaagtt tgacgtcggt gctgtgcgga agttagtggc ggagtcggcc
      961 cgcgatgttg acatcggcgg tccgcatctg gcggccgaag ccataaaggc caacatcgtc
     1021 gatgagtatc accacatcat agctcctgtg ctgattggca gtggtcttcg ctggctgccg
     1081 gaaggtgcgc acagccagct gcaattagtc agcacacgga cgttcgagaa cggtttcgtg
     1141 cacctgcact accgcaaaaa gatataaccc tggacgttta atggatctaa ctggaactga
     1201 tttcggatgc ctgtaagtgt ttcgacttta gcttgctaac ttagcgctcg ctacccttac
     1261 cgtactccgt gaagtagtaa tacccggccg taaatatgtt taagactatg aagtacacca
     1321 cccagtggtt tttggcttgc ggcggccaac gcaaaccggg cgggtcggtg ctcggtatat
     1381 cgtcgttgtg cagcatgtcg tggaacatcc aaagccagaa ggctgccagt acgaggggga
     1441 tgagaataac gatcagctta gccatcggtt atttgctggc gccggttcgc cgtttgttga
     1501 gcacccactg tacggaggga ctgatggctt tgtcgggacg gtggatgcaa cctatccagc
     1561 agcacggccg gcgcatgcct tcggtgagtt cgctgacggt gcgctttacg tggttctgcc
     1621 gggtcgcggc ggttttgacg gtgatggtcc aacagatcca ttcgttgcgg gccaggggag
     1681 tgatgctgtt ccaggcggcc agagccttcg ggctggccag gagggcttgg cgcatatcgg
     1741 ccggcagctt gtgcgcgacg ccgtcgtcga gaatctttgc ggtcataccc atatagtact
     1801 cgcgctcacc gccttattcc aggcagcacc agcgggggac tacgatggcc caaaagcagc
     1861 gagagtttga cgcgggcacc gggcgctacg ggtagagcgg accgactacg acgggcttgg
     1921 gcggcatatc gggcagggat acagcaggcg gattcggtga gtagaccgtc acttgggcat
     1981 cgaagtggtt gggcaagcac aagctggagc agtgacgatc ccactggtac tgtgtgttgt
     2041 ctatggtcaa tgtgccgttt ttgacactga ctttgatttt ggagggatcg gacttgtcgt
     2101 agtattttac ggccacgtag tatttatctg atgtttgcag gctgacagca gcttgggggc
     2161 cgaccacgtt cagtttggta aatggtgcta cattcttgac cagcgtattg aagtgcgcgt
     2221 tatagcggtc gcggaccctc gggacgacat tcgccgccag ggccgaacca ctgaccagac
     2281 ctatgacggt caggcccacc aacacaccgg tcagccaggt gcggatgggc cacttgcgcc
     2341 ggaagatggc catcccgaac aggatgatga acagcgtcat gagggcagcg acgaaaaccg
     2401 ccatgtaaac cagcagatgc tcctgaaaac cgatggggaa caagttgtcg taaatgacgt
     2461 tgccgtgcac cgacaggtag atgctgccga atgccaacaa ggccagcatg aacaagccga
     2521 acagtgtcag gccgatgccc actatcttca ccagcagccg gaaggcggtg tcgatgacgt
     2581 cgcgcacccg ggcggccggc cctgacaggg tgtcgttggc gcgccgggcg gcgcctttga
     2641 catcggcgcg ctcgactatc tctttcaggc tgtcgacggt gacgggacgg ccggccatct
     2701 gcaagcgctc gctgctcgtc tttgcctccg gtaccaacag ccacagc
//
LOCUS       DASSFD010000047         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_410447, whole genome shotgun sequence.
ACCESSION   DASSFD010000047 DASSFD010000000
VERSION     DASSFD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410447"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2188)
                     /locus_tag="VFH39_01040"
     CDS             complement(38..2188)
                     /locus_tag="VFH39_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6924400.1"
                     /translation="MSPDVTPPKTSDKPNKEPSEETASTASEPTKIAITDGDTDTTDT
                     APAETAETATPEPEAAEAEADEPVAEEAAEPEVMPEDSTDAPAGEDEAASEASETPEE
                     PAEPGLTDEQAASEAEVTPSADEAPEEAAVPAPEEQPAADTEEPAEETAAEPVVGAED
                     VKPAESQSESSEEATTVAPTAEAAAEPAPAATEPAAAPTPGPTPAAGHKASGVWKGLL
                     LLVALVVLAGGGWYAYTKYFHKQAAPVAATKKDIPMITYAVKAPGSSVGYPLIADTSV
                     NTAIDSQLFEGLVAYQDRSKIVPLLATGWTNPDNTTWLFTLHPNVTFHSGRLMTPADV
                     KSSLEYAMSHQKDNQGALAIASSIKSVDVVGSNQVKIITNGPDPTLLNRLTKLYIFDS
                     KAKLGDPNAGTGPYVVKAGTSMTPTSVYLTAVPHYWGGHVYTRAVHIVTQTDPQQMES
                     GLATGKYDLAGDFTAQALATLKPGNTITTTDVGTNFLGINTLKKDSPLASLKARQAAA
                     YALNVPAILAASKVNGVQASQLVPPSIPGYDPGIQNTPFDQQKARELLAQVPNSSAKL
                     TLSFPTSDELQATEIAKELNAAGFNVVPQKIDDFNQLIGVALGGKTDMFFLGYTSDTL
                     DGLDTLSSILTGNADYDNPQIDKLVQQAGSTLDPATRIQQLQEIAQLAAKDEPVIPVY
                     VKTTTFAVREHDYHVTADIPVQEVGVYFAKVYQE"
     gene            2557..2808
                     /locus_tag="VFH39_01045"
     CDS             2557..2808
                     /locus_tag="VFH39_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924401.1"
                     /translation="MSIAINEQVNVNAFYFGGRDMKTFPRRMEYRGQAVTFADGLRLL
                     VQRGNDLIQLFDMSAQDGSTYRLRREGDSWTLVGREVHA"
     gene            2840..3217
                     /locus_tag="VFH39_01050"
     CDS             2840..3217
                     /locus_tag="VFH39_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HET6924402.1"
                     /translation="MDDSETVANSGTRVALHAGFPNAAAERSSKPPLSLDQLLIRHPS
                     STYLFRVRGDHWLERGIFDGDIALIDRALPPRLGDLLLIWADEGFTLRAYTADYRPHQ
                     PWGVVTTVIHDYRGATNEADPVS"
     gene            3214..>3428
                     /locus_tag="VFH39_01055"
     CDS             3214..>3428
                     /locus_tag="VFH39_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HET6924403.1"
                     /translation="MKRPAIALIDCNNFFVSCERLFRPDLEGKPVVVLSSNDGCAVAR
                     SNEAKALGIPMGAPAFKYRELFRQQDVV"
BASE COUNT          588 a         1041 c         1052 g          747 t
ORIGIN      
        1 gggcccgcag tttaaaccgg aaccgtgcca taggtcacta ttcttggtac acctttgcga
       61 agtagacacc gacttcctgg acggggatat ccgccgtcac atgatagtcg tgctcccgta
      121 cggcgaatgt cgtggtcttg acgtagaccg ggatgacggg ctcgtctttg gccgccaact
      181 gggcgatttc ctgtaactgt tggatgcggg tcgccgggtc gagcgttgaa ccggcctgct
      241 ggaccagctt gtcgatctgc gggttgtcgt agtcggcatt gccggtcaga atgctgctca
      301 gggtgtccag gccgtccagc gtgtcgctgg tgtagcccag gaagaacatg tcggtcttac
      361 cgccgagcgc cacaccgatg agctggttga agtcatctat cttctgcggc acgacgttga
      421 agccggccgc gttcagctcc ttggcgatct ccgtagcctg cagttcgtcg ctggtcggga
      481 acgacagggt caacttggcc gacgagttcg gcacctgagc caacagctca cgggccttct
      541 gctggtcgaa gggtgtgttc tggataccgg ggtcatagcc cgggatggac ggcggcacca
      601 gttggctggc ctggacaccg ttcaccttgg aagccgccag tatagccggt acgttgagcg
      661 cgtaggccgc tgcctgccgg gccttcagcg aggccagggg cgaatccttt ttgagcgtat
      721 tgatgccgag gaagttggtg cccacgtcgg tcgtcgtaat ggtattgcct ggtttcagcg
      781 tcgccagcgc ctgggccgtg aagtcacccg ccaggtcgta cttgccggtg gccaggccgc
      841 tttccatctg ctggggatcg gtctgcgtga cgatgtgaac cgcccgggta tacacatggc
      901 cgccccagta gtgggggacc gccgtcaaat agacggaagt cggcgtcata ctcgtgccgg
      961 ctttgacgac gtaggggccg gtgccggcgt tcggatcgcc aagcttagcc ttggaatcga
     1021 agatatatag cttggtcagg cggttcagca gcgtcgggtc cggtccgttg gtgataatct
     1081 tcacctggtt cgatccgacc acatcgaccg acttgatgct gctggcaatc gccaacgccc
     1141 cctggttatc cttctggtgg gacatggcgt attccaagct ggacttcaca tccgcgggcg
     1201 tcatcagccg gccgctgtgg aacgtcacgt tcggatggag cgtgaacagc caggtggtat
     1261 tgtcgggatt cgtccacccg gtcgccagaa gaggtacgat cttgctgcgg tcctgatagg
     1321 ccaccagacc ttcgaacaac tggctgtcga tggcggtgtt gaccgatgtg tcggcaatca
     1381 gcggataacc gaccgaagag ccgggtgctt ttaccgcgta ggtgatcatc gggatgtctt
     1441 ttttggtggc cgccaccggc gccgcctgtt tatggaaata cttggtgtag gcataccagc
     1501 caccaccggc caatacgacc agggcgacaa gtaggagcag ccccttccac acgccggaag
     1561 ccttatgacc ggctgccgga gtcggcccgg gcgtcggggc ggcggctggt tccgtagccg
     1621 ccggcgccgg ctcagctgcc gcttcggcgg tcggtgcgac ggtcgtagcc tcttcgctgc
     1681 tttccgattg ggactccgcc ggcttgacgt cttcggcacc taccaccggt tcggccgctg
     1741 tttcctcagc gggctcttcg gtgtcggccg ccggctgctc ttcgggagcg ggcacagctg
     1801 cttcctcggg tgcctcatcg gccgagggcg tcacttccgc ctcggaggcg gcttgctcat
     1861 ccgtgagacc cggctctgca ggctcctcgg gcgtctcgct ggcttcgctg gccgcctcgt
     1921 cctcgccggc aggcgcatca gttgagtctt caggcatcac ttccggctca gcggcttctt
     1981 cggcgacagg ctcatcggcc tcagcctcgg ctgcttccgg ttccggcgtt gccgtttcgg
     2041 cagtctccgc aggagcggtg tcagtcgtat cggtgtcccc gtcggttatg gctatcttgg
     2101 tcggttcgct ggcggtactg gccgtctcct cggacggctc cttgttcggc ttatcgctcg
     2161 tcttgggagg cgtgacatca ggggacataa taggtgtttc tcttttacgt gataatactt
     2221 atgcttttag tatgattcct gccgttctgt tgtcaaactt ttggagtaat ttctacagca
     2281 gatagttgtg ctgacccaag caaaagcata tagtagcgga tagccatgtg agggctgttt
     2341 gccacccggc acgcttcctc ctcgcagact atttttcact cccaggttcg catactccgg
     2401 tcactgcgcg cctcctatat acaccaggta ccttagatga cgcttgcgag gacggctcgc
     2461 gacggacagg ttcctacccc tataaatccg taaccgctga ttcgtaacga atccgccggt
     2521 tctcttcatt attcgcaacg tacaggggga aatcttatga gcatcgctat caacgaacaa
     2581 gtgaacgtca atgcttttta cttcggcggc agggacatga aaacctttcc gcgccgcatg
     2641 gaataccggg gccaggccgt gaccttcgcc gacggcctcc gtctgctggt acagcgtggc
     2701 aacgatctta tccaactgtt cgacatgagt gcccaggacg gcagtaccta ccgcttacgc
     2761 cgggaaggtg acagctggac gctggtgggc cgggaagtgc atgcatgaaa acaaatacgc
     2821 ctaacgagga gatagactta tggatgattc agaaactgtg gccaacagcg gaacacgtgt
     2881 cgctctgcat gccggttttc ccaacgccgc ggccgagcgg agcagtaaac cgccgctcag
     2941 cctagaccag ctgctcatca ggcaccccag cagcacctac ttgttccggg tacgcggcga
     3001 ccactggctg gagcgcggta tcttcgacgg cgacatcgcg ctgatcgacc gggcgcttcc
     3061 cccgcgcctc ggcgacctgc tgctgatctg ggccgacgag ggcttcacgc tgcgcgccta
     3121 cacggccgac taccggccac accagccgtg gggcgtggtc acgaccgtca tccatgacta
     3181 ccgcggcgcc accaacgaag cggatccggt atcatgaaac gccctgccat cgcccttatc
     3241 gactgcaata acttcttcgt cagctgcgag cggctgttcc ggcccgacct ggaaggcaag
     3301 ccggtcgtgg tgctcagcag caatgacggc tgcgccgtgg cccgcagcaa cgaagccaag
     3361 gcgctcggca tccccatggg tgccccggcc ttcaagtacc gcgagctgtt ccggcaacag
     3421 gacgtcgt
//
LOCUS       DASSFD010000048         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_786307, whole genome shotgun sequence.
ACCESSION   DASSFD010000048 DASSFD010000000
VERSION     DASSFD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786307"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1475)
                     /locus_tag="VFH39_01060"
     CDS             complement(129..1475)
                     /locus_tag="VFH39_01060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET6924404.1"
                     /translation="MSEVKLEDIVSLCKRRGFIYPGSDVYGGLSGTWDYGPLGVALKR
                     NIMDLWWQMFVEDRDDMLATDAAILMNPKVWKASGHVHTFTDPLVECSNCHARHRADK
                     LDNPNKCPACGKENTFGEPRQFNMMFKTSVGPVEDEMSVSYLRPETAQGIFTNFKNVV
                     DSFYPDVPFGIAQQGKAFRNEISPRDFIFRTREFEQMEIEYFVHPDKWQEAFEMWVQL
                     VHAWTDALGLPKENVHELEVPEHDRAHYSKRTIDFEFDFPIGREELLGLAYRTDFDLR
                     NIEKGSGKSMEYRPKDGSPAFVPHVIEPSFGVERALMAVLSAAYTEDEVNGEKRIYLK
                     LPAHLAPVKAAVSPLLKNKPELVEKAREVYRALKNPQPGEGANWMQGAVMWDDNGNIG
                     KRYRRQDEIGTPECIVIDFQTLEDDTVTIRHRDTGNQTRMTIADLKIKSGPIEWTK"
     gene            complement(1731..>2019)
                     /locus_tag="VFH39_01065"
     CDS             complement(1731..>2019)
                     /locus_tag="VFH39_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HET6924405.1"
                     /translation="SLESWGAWLLGGLVVLLIGYFAVGIFGALGINIGGALTGWDWLG
                     VALFSALVVFDLNRAMRYPRTLDNAIDSAAAVFLDFLNIFIRLLELTGDSN"
BASE COUNT          370 a          581 c          621 g          447 t
ORIGIN      
        1 ggcatagccg tttaccgcca gcttacgcag cgccgcaata gagtccggaa acaatttgat
       61 ctttgcttcc tccgttaccc tgccgtccgg cggatcgact atcagcgtcc cgtcgcggtc
      121 gaagagtact actttgtcca ttcgataggg ccggatttga ttttcagatc ggctatggtc
      181 atgcgggtct ggttgcccgt gtcgcggtgg cggatagtga ccgtgtcgtc ctccagagtt
      241 tggaagtcta tgacgatgca ctccggcgtg ccgatttcgt cctggcggcg gtagcgctta
      301 ccgatgttgc cgttgtcgtc ccacatgacg gcaccctgca tccagttggc gccttcgccg
      361 ggctgcgggt tcttgagcgc gcggtagact tcgcgggcct tttcgaccag ctccggcttg
      421 ttcttgagca gcggcgacac ggcggctttg accggcgcca ggtgggccgg cagcttgagg
      481 taaatgcgct tttcgccgtt cacttcgtcc tcggtgtagg cggcgctgag cacagccatc
      541 agggcgcgtt cgacgccgaa ggatggctcg atgacgtgcg gcacgaaggc cgggctgccg
      601 tctttggggc ggtactccat gctcttgcca ctgcccttct ctatattgcg caggtcaaag
      661 tcagtgcggt aggccaggcc gagcagctcc tcgcggccga tcgggaagtc gaattcgaag
      721 tcgatggtgc gtttgctgta atgggcgcgg tcgtgctcgg gtacctcgag ctcatgaacg
      781 ttttcttttg gcaggccgag tgcatcggtc caggcatgga ccagctgcac ccacatttcg
      841 aaggcttcct gccacttgtc cggatgcacg aagtactcga tttccatctg ttcgaactcg
      901 cgggtccgga agatgaagtc gcgcgggctt atctcgttac ggaacgcttt gccctgctgc
      961 gcgataccga acggcacgtc cgggtaaaag ctgtcgacga cgttcttgaa gttggtaaag
     1021 atgccctgcg ccgtctcggg ccggaggtag ctcaccgaca tctcgtcctc gacggggccg
     1081 acgctggtct tgaacatcat gttgaactgg cgcggctcgc caaaggtatt ttctttgccg
     1141 caggccggac atttgttcgg gttgtcgagc ttgtcggcgc ggtgccgggc gtggcagtta
     1201 ctgcactcca ccagcgggtc agtaaaggtg tggacgtggc cgctggcctt ccagaccttc
     1261 ggattcataa ggatggccgc gtcggtggcc agcatgtcgt cgcggtcttc gacgaacatc
     1321 tgccaccaca agtccatgat attacgcttg agggcgacgc cgagcggacc gtagtcccac
     1381 gtgccactga gtccgccgta tacatcgctg cccggataga tgaacccgcg ccgtttgcat
     1441 aaactgacga tgtcttcgag ttttacttca ctcatgttcg tacctctgta atttagctga
     1501 aatttgaata tgcgaaaaga attacagtag gccgaagccc ctgccccgaa tgctcccgat
     1561 ggcggtgcga caggttttca tggcctgcat tatacccgtt atgtctctgt ttggcgaggc
     1621 ggtgagatag ctgataagtg cctaaaaatg aacagacccg atgcggatag cgtataaata
     1681 ctacccgcac cgggtgctgc atgttcagcg gccaggtgcc acgtctggga tcagttcgag
     1741 tcgccggtga gctcgagcag ccggatgaag atgttcagga agtccaggaa caccgcggcg
     1801 gccgaatcga tggcgttgtc gagcgtccgg ggataccgca tggcgcggtt gaggtcgaag
     1861 acgaccagcg cgctgaacag cgccacgccg agccagtccc aaccagtcag agcgccgccg
     1921 atgttgatgc cgagagcgcc gaagatgccg accgcgaagt agccgatcaa caggacgacg
     1981 agtcccccca gcagccaagc gccccagctt tcaaggctc
//
LOCUS       DASSFD010000049         5046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_591334, whole genome shotgun sequence.
ACCESSION   DASSFD010000049 DASSFD010000000
VERSION     DASSFD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591334"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            563..1018
                     /locus_tag="VFH39_01070"
     CDS             563..1018
                     /locus_tag="VFH39_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6924406.1"
                     /translation="MILVELILRYSKTPYPPIPDIPVLTPRPRRTPRVHAVRRRLQPD
                     VIDQLVADYQAGIDTTELTTRYRLGKGTVLKLLREAGVIMRRQGLSPAEIDEAVELYA
                     AGWSLARIGERFGRAHTVIRKALLDRGIAMHPGTANYLWGAAAGRHECH"
     gene            complement(1559..1635)
                     /locus_tag="VFH39_01075"
     tRNA            complement(1559..1635)
                     /locus_tag="VFH39_01075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1599..1601),aa:Val,seq:cac)
     gene            complement(1655..2941)
                     /locus_tag="VFH39_01080"
     CDS             complement(1655..2941)
                     /locus_tag="VFH39_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET6924407.1"
                     /translation="MIKLGTAKGLVPKKLLPFTFRTDRELTPEAVTEARKYIRDYWQH
                     VQRFHPKDDESLLGLPKPYLVPSYSEATGFDYNELYYWDSYFMVQGMLDAEHEELVKG
                     ILEDLMALFQRFKVIPNASRTYLMGRSQPPFLTTFIFDIYQAYDPGKEWLKEAISLAK
                     QEYQTVWLGQKKPNERLVHKGLSRYYDINYLHDLAEAESGWDMTPRFGRRALNYLPVD
                     LNALLYKYETDFARAARIFDDKREAAQWDAAANARKSTMNELMWDRIRGLYYDYNYVK
                     EKRGNVSSLAAYFPMWAGMVDGRQAASMVKALRRFENKGGLATTDALPVQQQFTIGAM
                     PTQWAYPNGWAPLHYIVIKGLERYGYHQDARRIANKWLKTNLWWFNEHHIFLEKYNVA
                     NPEKPPAKGVYPSQEGFGWTNAVFERLCQDYVDRAL"
     gene            3053..3637
                     /locus_tag="VFH39_01085"
     CDS             3053..3637
                     /locus_tag="VFH39_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET6924408.1"
                     /translation="MQFTLILLVFAVIFASELPDKSMIATLVLGTRYRALYVWIGAAA
                     AFLTHVIIAVVAGQALNLLPHRVVEFVVAALFLAGALLLLFGKHGLEEDPAPDAIKDG
                     HSFWKVAGTTYGVVFLGEWGDITQIMTANYVAHYHAPLSVGLGAVLGLWAATTIAVVA
                     GSKVLTRVPGKLLQRVVAAVLLLFAGLSLYSAIR"
     gene            complement(3884..4054)
                     /locus_tag="VFH39_01090"
     CDS             complement(3884..4054)
                     /locus_tag="VFH39_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924409.1"
                     /translation="MDIPESPPLPCADKLAFDTKKQADTAANVAAYQHGTKLKSYRCR
                     HCKLWHLASDYA"
     gene            complement(4076..4276)
                     /locus_tag="VFH39_01095"
     CDS             complement(4076..4276)
                     /locus_tag="VFH39_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924410.1"
                     /translation="MVKYGDKNDPEDAPERMQVHRVERKYRGHKLSRNVGHIFVERRH
                     GGESDKPNKHVDNPSGEPDRTL"
     gene            complement(4277..4639)
                     /locus_tag="VFH39_01100"
     CDS             complement(4277..4639)
                     /locus_tag="VFH39_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924411.1"
                     /translation="MLPDEPTDEERLEELPEDNETPFSAADSELDDTERTSGDGAVTQ
                     HVDDTHPVTDTDVQREDVYENGLADAAEAGEQPQKGAVLGYKGMPNDMADDAAGIDPP
                     AERIIEQDEDEQTTMEEV"
     gene            complement(4722..>5046)
                     /locus_tag="VFH39_01105"
     CDS             complement(4722..>5046)
                     /locus_tag="VFH39_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924412.1"
                     /translation="PTHGGMATPAMHDWLNDLPTQQLRGLDVAAFDTRVPSQWVKIIG
                     FASKRMATYLEKRGANLVALPMGFYVRGKTGPLKKGERERAKAWARQILQTMVIMHDH
                     GLGVA"
BASE COUNT          982 a         1506 c         1427 g         1131 t
ORIGIN      
        1 cttcagccgc ggcagcagcc tcttgctgac gagatttctc tgccaggata gaatcgcgcg
       61 cttttctaca ctcatcatct gcacgtcgtg cggccaagtc ggcctgcatt cgttcgtatt
      121 tccccatgcc agaactcctt cattaatatc ataacatatt atcgctccac cgcgtaatac
      181 atgttttcgg taccgcttcc accaaaccac catgttcacg ggcccaccac cactcaggtc
      241 gcttcggctg ccaccgtatc gacaaacccc tctacaagcg gtccaatcgc tacggcaaca
      301 accattccga cacgtgccat gatgcgacaa gccgcgcata agctgctgcc ggtcggcttc
      361 tacgatccta atgagctggc ttggtactgc agaaagttct ctgctggtgc ggtgcctcag
      421 atgtacaaga gcgaaagcta gtaagtcctg tttggtgagc aaaagcggct cgaaccccaa
      481 gtaataggtg gcaaagctaa aaaagctcag cattgctgcc gagcgatagg tacatattta
      541 ccccgagaac aagggggctg aaatgatctt ggtggagctc atattgcgtt actcgaaaac
      601 gccatatcca cccataccgg acatcccagt tctcacgcct cggccgcggc gcaccccgcg
      661 ggtccatgcc gtgcggcgac ggctgcaacc ggacgtcatc gaccagctgg tggcggacta
      721 ccaagccggc atcgacacca ccgagctgac cacgcgctac cggctcggca agggcaccgt
      781 gctgaagttg ctgcgcgagg ccggcgtgat catgcgccgg cagggcctct cccctgctga
      841 gatcgatgaa gcggttgagc tgtacgcagc ggggtggtcg ctagcgcgga tcggtgagcg
      901 gttcggccgg gcgcacaccg tcatccggaa ggcgctactg gatcggggca tcgcgatgca
      961 cccaggaacg gctaactact tgtggggcgc ggctgcaggc cggcatgaat gccactgatg
     1021 catagctggc cagcaacacg ctcgacctgc accgtccggt cggtgcctcg accgccgtta
     1081 tacatctggg caaaggagag ctgcactgac catccggagg gcgtcgcata gaagtcttcg
     1141 gggtctacct gggaatcggg atatatatcg gcggggaccc accgcacctg actgcctggg
     1201 cactccatac ttcgtgcgcg cgcgaattca tggtcccgct ccgctttggc aaacgccatc
     1261 gccttctgcg cagctctatc aacgactggg agcggcccgg ggattcgact ggcaacacat
     1321 tggcaccccg gatcgctatt ctcacaagcg ctcagtaaca aactagccgc tgctgcagca
     1381 ccaactgcca cagtacgtcc tgtccacgtt ccttccttgg acctcataag gctgattata
     1441 tagcaaatat cgaataaaag agacgtttta gttcgagtaa gcgtctcagg gtattgaagc
     1501 gaacggaccg gcgatcggac cacgtctacc cctgaagcaa ggcgtctgaa gttgatcttg
     1561 gtgggtccta cagggctcga acctgtgacc tcagcgatgt gaacgctgcg ctctagccaa
     1621 ctgagctaag aacccttaac agatggatta tacactacag ggcgcggtcg acgtagtcct
     1681 ggcacaggcg ctcgaagaca gcgttggtcc agccgaagcc ttcctggctc gggtagacgc
     1741 ctttggcagg tggtttttcg gggttggcga cattatactt ctcgaggaag atgtggtgct
     1801 cgttgaacca ccaaaggttg gtcttgagcc acttgttggc gatgcgacgg gcatcctgat
     1861 ggtagccgta gcgctccaag cctttgatga cgatgtaatg caacggtgcc cagccgttcg
     1921 ggtaggccca ctgggtgggc atggcgccga tggtgaactg ctgctggacc ggcagggcgt
     1981 cggtagtggc caggccgcct ttgttctcga accggcgcag ggccttgacc atgctggctg
     2041 cctggcggcc gtcgaccatg ccggcccaca tcgggaaata cgctgccagg ctgctgacgt
     2101 tgccgcgctt ttcttttacg taattgtagt cgtagtacag accgcgaata cggtcccaca
     2161 tcagttcgtt catggtgctc ttgcgggcgt tggcggcggc gtcccactga gcggcctcac
     2221 gcttgtcgtc gaagatgcgg gccgcgcggg cgaaatcggt ctcgtacttg tacagcaacg
     2281 cgttcagatc gaccggcagg tagttgagcg cccggcgccc gaaacgcggg gtcatgtccc
     2341 agccactttc ggcctcggcg aggtcgtgca agtagttgat gtcgtagtac cgcgacaggc
     2401 ctttgtgtac cagccgctcg ttgggcttct tctgcccgag ccataccgtc tgatactcct
     2461 gcttggcgag actgatggct tctttgagcc actccttgcc agggtcatag gcctggtaaa
     2521 tgtcgaagat gaaagtggtc agaaacggcg gctgcgaacg ccccatcagg taggtccgcg
     2581 aagcattcgg aatgactttg aaacgctgga acaaagccat cagatcttcc aggatcccct
     2641 tcaccagctc ctcgtgctcg gcatcgagca taccctgcac catgaagtag ctgtcccagt
     2701 aatacagctc gttatagtcg aaaccggtgg cttcgctgta gctcggcacc aggtacggct
     2761 tcggcagacc gagcaacgac tcgtcatcct tcgggtggaa gcgctgcacg tgctgccaat
     2821 aatcgcggat gtacttccgc gcttcggtga cagcctcagg cgtcagctca cgatccgtac
     2881 ggaaggtgaa cggcagcagt tttttgggca caaggccctt agcggtaccg agttttatca
     2941 tcttactccc agactacctg tgaccattgc catggtcaag ccgtaaaacc ggtaactttt
     3001 acagtagcga aacggcttac ggatacgcca tgatttgcta tactgaagcc tcatgcaatt
     3061 tacactcatc ctgcttgttt tcgcggtcat tttcgcctcg gaactacccg acaaatccat
     3121 gattgccaca ctggtgctcg gtacccgcta ccgggcgctc tatgtgtgga taggtgccgc
     3181 tgcggccttc ctgacgcacg tcatcatcgc cgtcgttgcc gggcaggcat tgaacctgct
     3241 gccgcaccgg gtggtggaat tcgtggtcgc cgcgttattc ctcgcggggg ccctactact
     3301 cctgttcggt aagcatgggc tcgaggaaga cccggcgccg gatgccataa aggacggtca
     3361 cagcttctgg aaagttgccg gcaccaccta cggcgtggtc ttcttgggcg aatggggcga
     3421 cataacccag attatgacag ccaactacgt agcccattac cacgccccgc tcagcgtcgg
     3481 tctcggcgcc gtccttggtc tgtgggccgc cacgaccata gccgtggtag ctggcagcaa
     3541 agtgctcacc cgggtgccgg gtaaactttt gcagcgggtc gtagccgccg tcttgctact
     3601 gttcgccggg ctcagcctat acagcgctat tcgctgaatt gatgcaaata gcacaaccat
     3661 tttttaagga tatgggtgga ctccgcgatt tataatacgt acatggcaac taatacaaag
     3721 agaggaggta atcaattggt aagtcctaga tctaacgggc agtcaatgag tagttttgcg
     3781 cgggaggtac gcgcactcta gccacccttt caaacgtgtt tggctgcctt agtaggccga
     3841 ataccttaac ccccgcacgg attgcattcg gacgggggtt ttcttatgcg taatcgctgg
     3901 ccaggtgcca gagtttacag tggcggcagc ggtacgattt gagcttggta ccgtgctgat
     3961 acgccgccac gttggcggcc gtgtcagcct gttttttggt gtcgaacgcc agtttgtcag
     4021 cgcaaggcaa agggggcgat tcgggaatat ccatcgcccc agtatagcaa ggctcctaca
     4081 gcgtgcggtc cggctcgccg gacgggttgt cgacatgctt gttcggcttg tccgactcgc
     4141 caccgtggcg tcgctctaca aaaatatgac cgacattccg tgacagttta tgcccgcggt
     4201 acttccgctc cacccggtgc acctgcatgc gctccggtgc atcttccgga tcgttcttgt
     4261 cgccgtattt gaccatctat acctcctcca tggttgtctg ctcatcttca tcttgctcga
     4321 ttatgcgctc tgcaggcggg tcgatgccgg cagcatcgtc ggccatatcg ttgggcatgc
     4381 ctttgtagcc gagcactgcc cctttctggg gctgttcacc cgcctccgcc gcatcggcca
     4441 agccgttttc gtagacatct tcccgctgca cgtcggtatc cgttacgggg tgcgtgtcat
     4501 ccacgtgctg cgtgaccgcg ccgtcaccac tggtgcgctc tgtgtcgtcc agctcggaat
     4561 cggccgcact gaacggcgtt tcgttatctt cgggcagctc ttccaggcgt tcttcgtcgg
     4621 tcggttcatc tggcagcatg tggcatctcc ttatgggtta cctgcccatg gtagcaacgc
     4681 atgctgctgg cataaatgaa atagcttact gacggcgctt cttacgcgac gcccagaccg
     4741 tggtcgtgca tgatgaccat ggtctgcagt atctgcctcg cccaagcttt ggctcgctcg
     4801 cgctcacctt tcttcaacgg ccccgtcttg ccccggacgt aaaagcccat cggcagcgcc
     4861 actagattgg caccccgctt ttccaaataa gtagccatcc gtttactggc gaaaccgatg
     4921 atcttgaccc actgtgatgg tacgcgggtg tcgaaagccg ccacgtccag cccgcgcagc
     4981 tgctgggtgg gcagatcgtt gagccagtcg tgcatcgccg gggtcgccat accgccatgg
     5041 gtcggc
//
LOCUS       DASSFD010000050         4637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1354287, whole genome shotgun sequence.
ACCESSION   DASSFD010000050 DASSFD010000000
VERSION     DASSFD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354287"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..654
                     /locus_tag="VFH39_01110"
     CDS             46..654
                     /locus_tag="VFH39_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET6924413.1"
                     /translation="MSTAPSRLAKVLPWLLLIGGAIGLICSFVISLDKQKLAQNPSFR
                     PNCDLNPIVSCFNVMKSHQGSVFGFSNPWIGLAAFAALMTVAVALLAGGRFKRWFWQG
                     LEAGMVFGMVFAYWLLYESVYTIRALCPYCLVVDVVVTTMFWYTTLYLAEQHMLPAAG
                     GARATKAVAWSRQHHLDLLLLWFLIVIFFVVKHFWYYFGSHL"
     gene            690..2063
                     /locus_tag="VFH39_01115"
     CDS             690..2063
                     /locus_tag="VFH39_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET6924414.1"
                     /translation="MNKNEAILKKSVEVPAELLDRTITTTQALTKGVPAGKVIRKTED
                     YWHLLGPGLTTGASDDDPSGIATYSQTGAQYGFRFLWLSPLTFPLMAAVQEMCARIGL
                     VTGRGLAGNIRIHFSRRLLLVCTVLLFAANAFNIGADIGAMAKGVQLLAPHFSFTATV
                     IAISLISLLVQIFTPYVRYARYLKWLAMLLLAYVFSALLAHLNWSDIFRHTVVPQVGF
                     NKSELLLVAAILGTTISPYLFFWQTSQEVEEQILQGKTSVKSRQKETTPADIKSMRID
                     VWSGMFLSNVVMFFIIAACGGVLFKHGITNITSAAQAAQALRPFAGNATTFLFALGII
                     GTGFLAIPVLAGSSSYALAESFGWREGLYRRLGQAYAFYGVIIISMLVGLGINFIGLD
                     PIKALIYAAVGNAVAAPVILALIVLLSSNRKVMGKWTNKPSTTIIGWLVTLLMTVASI
                     AAIVSLF"
     gene            complement(2151..3374)
                     /locus_tag="VFH39_01120"
     CDS             complement(2151..3374)
                     /locus_tag="VFH39_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6924415.1"
                     /translation="MKSLTRGNFKAAMSSLRQNKWRSFLTMLGIIIGVSSVITVVSIG
                     QGIKHQVSVNIQHVGPNLITVLPARTEAGGLQSLGSLVGVGGNGTLSEKDYEAVVASH
                     DVAQAAPLGILNGTVTGERGTYTAGPVVMTSADFPSLVNQTVGYGAFISQDDGDNNVA
                     VLGSSAATKLFHASVPLGQTFTFRGQQFMVRGVLNDAPNAPLSSAVDYNNAIFISYAT
                     GLKLTNGSATVAEVLAKSASPDPQAAIKAIRSNLTRLHGDSQDFSVLVGAQAEARTDR
                     VLGLLTELISGVAAVSLLVGGIGIMNVLLVSVTERQHEIGIRKAVGATDQQIMSEFLS
                     EATVLSVVGSIIGIIVSLAVNLLLRIFTSLQPAFDWQIIVIATLVSIAVGILFGTAPA
                     VKAARKDPITALRGE"
     gene            complement(3371..4249)
                     /locus_tag="VFH39_01125"
     CDS             complement(3371..4249)
                     /locus_tag="VFH39_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6924416.1"
                     /translation="MPLITIKDVSKLYGFGDATTLALDEVSLSIEPGEFVAVMGPSGC
                     GKSTLMNLIGLLDRPTHGTYQLNGRDVSRLKPNQSAKIRRDTIGYIFQSFNLLPKLTA
                     LENVALPLLYKNMTTTRRLKQASSILERVGMQEREYYLPSQLSGGQVQCVAIARALVN
                     NPKLIIADEPTGNLDSAGSRLIMEMLSEIHKLGNTILFVTHNPELTRYATRVLYMHDG
                     MIIQDEKTAIGQVARTARRSMFTLPATTEEDDLAGVSALMKAVPDKEAKTPRKTTKRK
                     TTGRGKVTRPKKKAAK"
     gene            4456..4545
                     /locus_tag="VFH39_01130"
     tRNA            4456..4545
                     /locus_tag="VFH39_01130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4490..4492,aa:Ser,seq:gga)
BASE COUNT          891 a         1348 c         1333 g         1065 t
ORIGIN      
        1 taagcgctat ttatccgcgg tgccgaagcg gttatactag gggtaatgtc taccgctccc
       61 tcacgccttg ccaaggttct tccgtggctg ctgttaatcg gcggcgccat cgggcttatc
      121 tgttcatttg tcatttcact cgataagcag aaattggcgc agaatccgtc attccgcccg
      181 aattgcgacc ttaatcccat cgtgagctgc ttcaacgtga tgaagtcgca ccagggttcc
      241 gtgttcggat tttccaatcc ctggatcggc ttggctgcct tcgccgccct tatgaccgtt
      301 gcggtcgccc tactggccgg gggacgtttc aagcgctggt tctggcaagg cctggaagcc
      361 ggcatggtgt tcggcatggt gtttgcttac tggttgctgt acgagagcgt ctacaccatc
      421 cgggcgcttt gcccgtactg cttggtggta gacgtcgtcg tcaccaccat gttctggtac
      481 accacgctgt atttggcgga acagcatatg ctgccggcag caggcggagc ccgtgccacg
      541 aaggccgtcg cctggagccg ccagcatcac ctcgacctgt tactgctctg gttcctgatc
      601 gttatcttct tcgtcgttaa gcacttctgg tattacttcg gcagccattt gtagacattt
      661 gctatactgg ctgcagtaca actaaaaaca tgaataaaaa cgaagcgata ctgaagaaaa
      721 gcgtcgaggt cccagccgaa ctgctcgacc gcaccattac gaccacgcag gcgctgacca
      781 aaggcgtccc agccggcaag gtcatccgta agaccgaaga ttattggcat ttgctcggac
      841 ccggactgac caccggcgcg tccgacgacg acccctccgg tatcgcaacc tacagtcaga
      901 ccggcgccca gtacggcttc cgcttcctgt ggctgtcacc gctgacattc cccctcatgg
      961 ccgccgtcca ggagatgtgc gcccgcatcg gcctggtgac cggtcgtggt ctggctggca
     1021 atatccgcat ccatttttcg cgccgcctgc tgctggtatg cacggtatta ctgttcgccg
     1081 ccaacgcttt caatatcggg gcggacatcg gtgccatggc caagggcgtg cagctgctgg
     1141 ccccgcactt cagcttcacg gccaccgtca tcgccatctc actcatcagc ctgttagtcc
     1201 agatctttac accatatgtg cgctatgcca gatatctcaa atggctggcc atgctgctgc
     1261 tggcctacgt gttttcggca ctgctggcgc atttgaactg gtcggatatc ttccgtcata
     1321 ccgtagttcc tcaggtaggt ttcaataaaa gcgagctcct gcttgtggcg gcgatcttag
     1381 gcaccaccat ctcgccgtac cttttctttt ggcagacctc ccaagaggtc gaggaacaga
     1441 ttttgcaggg caagacatcg gtaaagtcgc ggcagaaaga aaccacgccg gccgacatca
     1501 aaagtatgcg catcgatgtc tggagcggca tgttccttag caacgtcgtc atgttcttta
     1561 tcatcgctgc ttgtggcgga gtactgttca agcacggcat caccaacatt acgtcggcgg
     1621 cccaggccgc ccaggcgttg cgcccgttcg ccggcaacgc caccacgttc ctgttcgcac
     1681 tcggcatcat cggcaccggc tttttggcta ttccggtatt ggccggttcg tccagttacg
     1741 ccctggccga gagcttcggc tggcgggaag ggctataccg acggctcggc caagcctatg
     1801 cgttttacgg ggtcatcatc atttccatgc tggtgggact gggtatcaac ttcatcggtt
     1861 tagaccccat caaagcgctc atctacgccg ccgtcggcaa tgccgtagct gcccccgtca
     1921 tcctggcgct tatcgtattg ctcagcagca accggaaagt gatgggtaag tggacgaaca
     1981 agccgtctac gaccattatc ggatggctag tcacgctgtt aatgacggtc gcatccatcg
     2041 ccgccatagt atcgctgttc tgagttagct aggccggaat cgtatctgac cgtctgaacg
     2101 acatatctgt gctgccggac caggcgcaga tgatgtactt gtagtatggg ctattcgccg
     2161 cgcagggcag tgatggggtc tttgcgggcc gccttgacgg ccggggcagt gccgaagagg
     2221 ataccgacgg caatcgatac cagcgtggcg atgacgataa tctgccaatc gaaggccggt
     2281 tgcaaagaag tgaagatgcg tagcaacagg ttgactgcca gcgatacgat gatgccgatg
     2341 atactgccga caacgctcaa caccgtcgcc tcgctcagaa actcgctcat gatctgctga
     2401 tcggtcgcgc cgacggcttt gcggatgccg atttcgtgct gccgctcagt cacggacacc
     2461 aagagcacgt tcatgatgcc gatgccgccg accagcaatg acaccgccgc cacgccgctg
     2521 ataagttccg tcagcagccc cagcacccgg tcggtacgag cctcggcctg tgcaccgacc
     2581 agcacgctga agtcctggct gtcgccgtgc aggcgggtca gattactacg gatggctttg
     2641 atggccgcct gcgggtccgg tgaggccgat ttcgcgagca cttccgccac cgtcgcgctg
     2701 ccattggtca gtttgaggcc ggtggcgtag cttataaaaa tggcattgtt atagtcgacg
     2761 gccgaggaca gcggggcgtt gggcgcatcg ttaagcactc cccggaccat gaactgctga
     2821 ccgcggaagg taaaggtctg accgagcggg acgctggcat ggaacagttt ggtggcagcg
     2881 ctggagccga gcacggcgac gttattgtca ccatcgtcct gcgatatgaa agcgccgtag
     2941 ccgacggtct gattgaccag gctcgggaaa tcggcggagg tcataaccac cggtccggca
     3001 gtataggtac cgcgctcacc ggtaaccgtg ccgttcagta taccgagcgg cgcggcctgg
     3061 gcgacgtcat gactcgctac caccgcttcg taatcctttt cggacaaggt gccgttaccg
     3121 ccgacgccga cgaggctgcc aagtgactgc aatccgcccg cttcggtgcg cgccggcagc
     3181 accgtgataa ggttaggccc gacgtgctgg atgttcacgc ttacctgatg tttgattcct
     3241 tgaccgatgc tgaccaccgt gatgaccgag ctgacgccga tgatgatgcc cagcatcgtc
     3301 agaaagctgc gccacttgtt ctggcgcagg ctgctcatgg cggccttgaa gtttccgcgg
     3361 gtcagcgatt tcatttggca gcctttttct tagggcgcgt gaccttgccg cgaccggtcg
     3421 tcttacgttt ggtggtctta cgaggggtct tagcttcttt gtccggaacg gccttcataa
     3481 gtgccgacac gccggcgaga tcgtcttctt cggtggtggc cggcaaggtg aacatcgagc
     3541 gtcgggcggt gcgcgccacc tggccgatgg ccgtcttttc gtcctggatg atcatgccgt
     3601 cgtgcatgta cagcaccctc gtagcgtaac gggtcagttc cgggttgtgc gtgacgaaca
     3661 ggatggtgtt gccgagtttg tggatttcgc tgagcatttc catgatcaac cgcgaaccgg
     3721 cgctgtccag gttaccggtc ggctcatcgg cgatgatgag cttcgggttg ttaaccagcg
     3781 cccgggcgat ggcgacgcac tgcacctggc cgccgctcag ctggctgggc aggtaatatt
     3841 cccgttcctg catgccgacg cgctccagta tggaactggc ctgcttgagc cggcgggtgg
     3901 tggtcatatt cttatacagc aggggcagag cgacattctc gagcgcggtc agtttgggca
     3961 gcagattgaa cgattggaag atataaccga tggtgtcccg gcgaatcttg gccgactggt
     4021 tcggcttcag ccgtgagacg tcccgaccgt tcagctgata cgtgccgtgc gttggccggt
     4081 ccagcaagcc gatgaggttc atgagcgtgc tcttgccgca gccgctcggc cccatgaccg
     4141 ccacgaattc gcccggctcg atgctcaagc tgacttcgtc gagcgccaga gtagtagcgt
     4201 cgccgaagcc gtacagtttg ctgacgtctt ttatcgtaat aagaggcatt gctatctagt
     4261 gtaaacctgt cataacttag tcacaacctg tttttgtagt ataattcgca ctttcttatc
     4321 tgtaacgatt atgcttgacg gcacggaacg gttgccataa tcgacggtaa gtacgctata
     4381 attgagcagt tcgtcgaccg gataccaaaa tcgcagcctc gcaagcacag gcgacggaat
     4441 gcgattttga aaccaggagg ggtcgcctag tggtctatgg cgccgccttg gaaaggcggt
     4501 ttactcgcaa gggtatcgag ggttcgaatc cctctccctc cgccaacata aaaggagctc
     4561 aacacggagc tccttttatg ttgaccgctt acttcattca cgaggccgcg aaaactctta
     4621 tggtacgtcc gctgtag
//
LOCUS       DASSFD010000051         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_156830, whole genome shotgun sequence.
ACCESSION   DASSFD010000051 DASSFD010000000
VERSION     DASSFD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156830"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1754
                     /locus_tag="VFH39_01135"
     CDS             312..1754
                     /locus_tag="VFH39_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924417.1"
                     /translation="MSAPHNPNGTTVWTPDGGRWSDGTPVGPKHMLDAARVEEIELDE
                     AARELTAMGNNRTPAPNATTIVQLASGWPTETATSAERKQYPLPDLGRLPKEGRPGKR
                     VRRLLESIDRTQEDPQNPGFALVPQAVRDSIPAPTLEEQARWDAEDYRRRTEALIDYT
                     GLLAKQKRGAKVLFLGEIGGSGTTTFCTHLAASLRVDTQADTLVYDANHSMGSVAKRF
                     GFYGSDTLTQQQLSELRLWYGADKQNFSDDTPNHTRLGVRVVASSRIWDGERLLFRAN
                     DCSECLKIMSSNTKFLFIDAMNDFTHEVGRRLLSEVDLIVFTASTRKEDSLWSLWPMM
                     DLVRELGFEELVKRSIVGITGYPAGTNLKAAYGAFLKHRELRKQGGREVVQEFKNDRV
                     YDVPMIGIPHDPHVESERIDPIDRLQLATREAIREGVIAVLQKLPDRRQLTRQQVLAW
                     VQQQRSAHPGQLQPTGLQRGLEPVAQANGH"
     gene            2311..3234
                     /locus_tag="VFH39_01140"
     CDS             2311..3234
                     /locus_tag="VFH39_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924418.1"
                     /translation="MGRFEVQEDAMLGLETIKHPGRIATASLAALAMTGGAFAACGAS
                     ETVAHAGHASLESVTLEKGTYCDASFVADIQDVEASFTKDLPGPFNYGRSVHLSERLQ
                     ATICVKAESIDAKYDSKVGKYVVNLVASPDPNKSDLVLRISSADEYRQITHGGSLVQK
                     WGNLGVDVVNSFGLNLEPNANIDDALLQVASLVADEQAAKTCEPVALPYLTKHNSVVT
                     NENTGPNAPQTMQQDIAQNMLIRALNMDSHNHYTLNDFEVKIHAEPGGLADQYQKQYE
                     EMLKNGPDKGLHVPDDSQLPKCTNELVKHHG"
     gene            3227..4063
                     /locus_tag="VFH39_01145"
     CDS             3227..4063
                     /locus_tag="VFH39_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924419.1"
                     /translation="MDKTDNFERFYSTPGPDGTEPIVSFDPTPVPLVSDVEATGYIPR
                     IEAPIAEHPTQTIKAVTDVEPVTVPVTRADGSVWEIDTHPTTGKMIGSPRLIQQAPAV
                     RMVYEDHGHSVSFSANVDRRRGPAAAHALHAAAQQLSGNRANAVPFMPVMPGMGVPAY
                     SHPAAIPGMMPPYAAPVTETSRPNFEKPAPTSQSTTEADAQVTRKPQSAETTGTAARL
                     VPKIVKRGIGIVMLTYAMDFIGYNAVSFVSHPTHLADITGNIRVSKNPFIGFEDMGKI
                     LG"
     gene            4067..>5353
                     /locus_tag="VFH39_01150"
     CDS             4067..>5353
                     /locus_tag="VFH39_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924420.1"
                     /translation="MANTNHNPNTHAERQLEEYLARNGVPEAQQPDTFTEQTTQLPEY
                     YGYPQAQMPYGQSYTPWPGYYQAPVLSGMPQAGAERLSPTGRDETLESLHKELREIRE
                     QSTRAADHRESSEENKKYGRTAMAIMAGVVILGGAWYVTTGHSKPSATADKNGLPSKS
                     PLPSHHQSLTPHNSEAPISPSTSATVTTSPSASSSASPSPSESSTAEPTTSPSSEPTR
                     HHYKAFVPEALPGTSIAAVKVQSMPVPLTEMLQYPSDPTHLWKITGATNNFKASLAGN
                     AELAIGSIPASALTLDAQSTKLTNMTTYQLSPTDISLHLDASSYSIAAGPTIDPKNNI
                     PFYLESLCKEAKPAKTNTYCVAVENYADLTSNNPATVSAAVNKYPDANAVITGTEAQL
                     TDQSRTSEHADTPLTDGMSMLLASEMNAQPSLKQEVE"
BASE COUNT         1354 a         1567 c         1429 g         1003 t
ORIGIN      
        1 accacatatc aggtttttgg ctctcgcaac gtcttcactg tcggcgtgta caaataaatt
       61 gctcatttgc aagcgagttg cttaattgta aattatatag tagggtaatg ctaacgcacc
      121 ggaggggtgt gaaggtttcg atccgatcct tgcactgacc tctgttattt agtgtaacga
      181 ccggaccaaa atccggcctt cttgctcgcc accatcgcct accaaggaag gaggctcgag
      241 caatttaaca gaagggaaac gcaccttaag ttattggcaa agcaagccct acacgagagg
      301 aggtgtggac catgtccgca ccgcacaacc ccaacggcac gaccgtgtgg acaccggatg
      361 gtggacgctg gtccgacggc acccccgtcg gcccgaagca catgctcgac gccgctcgcg
      421 tcgaggaaat cgaactcgac gaggcagcac gcgagctcac tgcaatgggc aacaaccgca
      481 cgcctgcccc gaacgcaacg acgattgtgc aactcgccag cgggtggccg accgaaaccg
      541 ccacatccgc cgagcggaaa caatacccgt tgcccgacct cggtcgactg ccgaaagagg
      601 ggcgaccggg taagagggtc cgccgcctgc tcgagagcat cgatcgcacc caggaagacc
      661 cgcagaaccc cggcttcgcc ctggttccgc aggccgttcg ggacagcatc cctgcgccta
      721 cgctcgaaga gcaggcacgc tgggatgccg aagactaccg acgtcggact gaggcactca
      781 tcgactacac cggtctgctc gccaagcaga agcggggcgc gaaagtgttg ttcctcggcg
      841 agatcggcgg tagtggcacg accacgttct gcacccatct tgctgcctcg ctgcgggtcg
      901 acacccaggc cgacacgctg gtctacgacg ccaaccattc catgggcagc gtcgcgaagc
      961 gcttcggttt ctacggcagc gacacgctca cccagcaaca gctttccgag ttgcggttgt
     1021 ggtacggagc cgacaagcag aacttctcgg acgacacccc gaaccacacc cggctcggcg
     1081 tccgcgtcgt cgcatccagt cgcatctggg acggcgaacg gttgctgttc cgggccaacg
     1141 actgctccga gtgcttgaag ataatgagct cgaacaccaa gttcctgttc atcgacgcca
     1201 tgaacgactt cacgcacgaa gtcggccggc gattgctgag cgaggtcgac ctgatcgtct
     1261 tcaccgccag cacacggaag gaggattcgc tgtggtcctt gtggccgatg atggacctgg
     1321 tccgtgagct cggcttcgag gagctggtca agcgcagcat cgtggggatc acgggctacc
     1381 cagccggcac aaatctcaaa gcggcgtacg gcgccttcct caagcatcgc gagcttcgca
     1441 agcaaggtgg aagggaagtc gttcaggaat tcaagaacga ccgtgtgtac gacgtgccga
     1501 tgatcggcat cccgcacgac cctcacgttg agagcgaacg gatcgatccg atcgatcgcc
     1561 tgcaactggc tacccgagag gcaatccggg aaggcgtcat cgccgttcta cagaagctgc
     1621 ccgaccgtcg gcagctgaca cggcagcagg tcctcgcctg ggtccagcag cagcggtccg
     1681 cacacccggg tcagctgcag cccaccgggc tgcaaagggg gctcgagcca gtcgcacagg
     1741 caaacggtca ctgaagtcaa cagcacgcga accagcgtgc cgccagcggc cggggccggc
     1801 agatcgggcg agggaaagca gttcccttcg cgcgtgacgc cggccccaag gccgtgcttt
     1861 gccagtccct ttgctaaggg gcgtttcaac ttacggccaa ccggggccca cggctgcaca
     1921 tacgcagcgc tatacccggt aacaccctaa gggcttaaga ttatcgctta agtttttagg
     1981 gtgtttttat gtcttttatg cataagcata ttcaccggat attggctgta agatttttta
     2041 tttttttgtt gacatgtcaa attattgtga ttatagtctg cctttaacaa gggtagttgc
     2101 atgacgagcg gagtgggtaa cgtcggttaa tcgtccgagg tcatgcaact acactctctc
     2161 gcaagaggga agcgtgattg catgaacgac agcggatgtc gcccaccaaa gcggcagcag
     2221 gtcggagtca tgcgtcacgc acgccaggaa ctccacttcc tataacaggc ccgcagtcgg
     2281 ttatataact cgtaagccct gaccattggt atgggtcggt tcgaagtaca ggaggacgct
     2341 atgctcggtt tggagactat taagcaccca ggcagaattg ccactgccag cctcgctgcg
     2401 ctggcaatga ccggaggcgc tttcgcggct tgcggcgcat cggaaaccgt agcccacgcc
     2461 ggccatgcat ccttagaatc agtcacgctc gaaaagggca cctactgcga cgccagcttc
     2521 gtagctgaca ttcaagatgt cgaagcaagc ttcacgaaag atctgccggg gccattcaac
     2581 tatggtagat cagtccattt gagtgaaaga ctgcaagcca ccatatgtgt caaggcggaa
     2641 tcaatcgacg ccaaatatga ttcgaaggta ggcaagtacg ttgtcaacct agttgcatcg
     2701 ccggatccaa acaaatcaga cctagtgttg aggatttcgt ctgcagacga ataccggcaa
     2761 atcacccatg ggggcagcct tgtgcaaaaa tggggaaact tgggcgttga tgtggttaat
     2821 tcgttcggcc ttaacctcga acccaacgcc aatatcgatg atgcgctatt acaggttgcc
     2881 agcctagtgg ctgacgaaca agcagcaaaa acctgcgagc cggttgcatt gccatacctc
     2941 actaaacata actcagtagt aaccaacgaa aataccggac cgaacgcacc ccagaccatg
     3001 caacaagaca tcgcccagaa catgttaata cgtgccctca atatggactc acacaaccat
     3061 tacacgctca atgatttcga ggtcaagatt catgccgagc caggaggctt agccgaccag
     3121 taccagaagc agtacgagga aatgctcaag aacggaccgg ataaaggtct acacgtaccc
     3181 gacgacagcc agttgcctaa atgcacgaac gaattggtta agcaccatgg ataagacaga
     3241 taacttcgag aggttctatt caacgccggg tcccgatggc accgaaccaa ttgtgagctt
     3301 cgaccctacg ccggtaccat tggtatcaga cgtggaagca accggataca taccgcgtat
     3361 tgaagcaccg attgccgagc atccaacaca gaccattaag gcagttaccg atgttgaacc
     3421 agtaaccgta ccagtaacaa gggcagatgg cagtgtgtgg gaaattgaca cccaccccac
     3481 aacaggcaaa atgatagggt caccgcgatt aatccagcaa gcgcccgctg tgaggatggt
     3541 ctacgaggat catgggcata gcgtaagctt tagtgccaat gttgatcggc ggcgtggacc
     3601 ggcagctgcc catgcgctgc acgcagcagc gcaacagctt agcggtaaca gggctaacgc
     3661 cgtcccgttc atgcctgtaa tgccaggcat gggggtgccc gcttattcgc atcctgcggc
     3721 cattccaggc atgatgccac catatgcagc acctgtaact gaaacgtccc gacctaattt
     3781 cgaaaaacca gcgccaacct cccagagtac aacagaggcg gacgctcagg tcacgcgtaa
     3841 gccacaatcc gccgaaacga ctggcacagc agcgcgcctg gtgccgaaga tagtaaagcg
     3901 tggcatagga atagttatgc tgacctacgc catggatttt ataggctaca acgctgtttc
     3961 gttcgtttcc catccgacac atttggcgga tataaccggg aacatacgcg ttagcaaaaa
     4021 tcctttcatc ggctttgaag acatgggaaa aatactgggc taatctatgg caaatactaa
     4081 tcacaatccg aacactcacg cagaacgtca actggaagaa tacctcgcta gaaatggcgt
     4141 accagaggca cagcaaccag acaccttcac cgagcagacg actcaattac ccgaatatta
     4201 cggctacccg caggcgcaaa tgccttacgg tcagagctat acaccgtggc ctggatacta
     4261 tcaggcacct gtcctttcgg gtatgccaca agcaggcgcc gaacgcctgt cgcctacggg
     4321 gcgtgacgaa acgttggaaa gcctccataa agaactgcgt gagatccggg aacagagcac
     4381 ccgtgccgct gaccaccgcg aaagcagtga agagaacaaa aagtacggtc gtacggccat
     4441 ggcaataatg gcaggggtcg tcatacttgg gggcgcctgg tacgtaacta caggacactc
     4501 caagccgagt gcgacggccg acaaaaacgg cttaccgtcg aagtctccgc tgccatcaca
     4561 tcaccaatct ttgacgccgc ataactcaga agctccgatc tcaccatcaa cgagcgccac
     4621 tgtgacaaca tcaccgagcg cttcgagtag cgcgtctccg tcgccgagtg aatcatcgac
     4681 agccgagccc accacttcgc catcgagcga gccaacccgg catcactaca aggcattcgt
     4741 accggaggca ctgccgggca ccagcattgc ggccgtcaag gtgcagtcca tgccggtgcc
     4801 gctgaccgaa atgctgcaat atccgtccga tccaacgcat ctttggaaaa taacgggagc
     4861 taccaataac ttcaaagcaa gtctcgctgg taacgccgag ttggccatcg gttccatacc
     4921 agcgtccgcg cttacgctgg atgctcaaag cactaagctc accaatatga cgacatacca
     4981 gctatcgccg accgatatat ccctccacct tgatgcttcg tcgtacagca ttgccgctgg
     5041 tccgaccatc gatcccaaga ataacattcc gttctacctg gagtctcttt gcaaggaagc
     5101 caaaccggct aagacaaata cgtactgcgt ggcggtagaa aattatgccg atcttaccag
     5161 caacaaccct gccaccgtat cggccgcagt caataagtac cccgatgcga acgccgtcat
     5221 taccgggaca gaggcgcaat tgaccgatca aagccggaca tccgagcatg cggacacgcc
     5281 actgaccgat gggatgtcta tgctattggc cagcgagatg aatgcgcagc cgtcgttaaa
     5341 gcaagaggtg gag
//
LOCUS       DASSFD010000052         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_302445, whole genome shotgun sequence.
ACCESSION   DASSFD010000052 DASSFD010000000
VERSION     DASSFD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302445"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFH39_01155"
     CDS             complement(<1..216)
                     /locus_tag="VFH39_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924421.1"
                     /translation="MSTEVNIHAAQTSILRELLFRRAAGFAELQKPTGLTSDHFNFHM
                     SRLLELGLIEKVSRGTYRLTSRGKEYAN"
     gene            complement(300..797)
                     /locus_tag="VFH39_01160"
     CDS             complement(300..797)
                     /locus_tag="VFH39_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924422.1"
                     /translation="MEDTAQQFTASKADRPRFNPVLLVLVLLGIGLVVALALNWANKQ
                     KLNQEIASAKQLSDRTLHDIKTQNGADARSFADASFRKQYPADALSTRFRLADQVLKG
                     TPTLDRRTLVQGSKNDTVFFIYKYRNGGAAYYIRTGISNSGKNHTWQLIDIESDTDES
                     HLLIK"
     gene            856..2100
                     /gene="serS"
                     /locus_tag="VFH39_01165"
     CDS             856..2100
                     /gene="serS"
                     /locus_tag="VFH39_01165"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6924423.1"
                     /translation="MLDIQFIRDNPELVQRKSEEKGYHVSVAELLELDTERRSKLTQI
                     EELRSRRNSLAAEMKNQRPTDEQIAAGKQLKEEIAAREAELQTIDDKYGAQLMAVPNV
                     TFDDVPVGGEEDSVEIKVVGEKPTGAKDHLDYATERGWLDFEAGAKVAGTKFYYLKGD
                     LALLENAITQYALNFCVDKGFTYMTVPHMVNSRIATGAGFAPRGDKEGNEYFVENEDL
                     TMIGTAEAPLTGYHADEILDESALPKLYVGYSPCYRREAGTYGKHTRGLFRVHQFNKL
                     EMYAFTLPENSQEMHERILAIEEELWQSIGVPYHVINIASGDLGAPAAKKYDIEYWSK
                     ADGAYRELTSCSNCTDFQARNLNIRVRRRDGSIETLHTLNGTAVSLARSLVVVLEYFQ
                     NEDGSLTVPEVLRPYMGGRECI"
     gene            2103..2465
                     /gene="raiA"
                     /locus_tag="VFH39_01170"
     CDS             2103..2465
                     /gene="raiA"
                     /locus_tag="VFH39_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6924424.1"
                     /translation="MLTHVVQAEGYSVSGELQKYTQKKLDSLERYIPRSCRRSAHVEV
                     RFKQKASKDKNRKFNTCELTLHLPGDTLFAKETTQHMYAALDIVAVHIKQQLDDYRAK
                     HSPRWRHRLARRLGGEWQ"
     gene            2565..>2821
                     /locus_tag="VFH39_01175"
     CDS             2565..>2821
                     /locus_tag="VFH39_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924425.1"
                     /translation="MNAVLKKLLGDPQAKTIKRLRRRVEAVNKLAPKYEKMSDADLRK
                     QTDVLRKRLKKESLDAILPDAFAVVREAATRSLGMRHFDVQL"
BASE COUNT          686 a          771 c          815 g          549 t
ORIGIN      
        1 gttggcgtat tctttgccgc gtgatgtcag acggtatgta ccgcggctga ccttttcgat
       61 aaggcccagc tctaacagcc gcgacatatg gaaattgaaa tgatcgctcg tcagtccggt
      121 cggcttctgc agctcggcaa agccggcggc gcggcggaac agcagctcac gcaggatact
      181 tgtctgcgcg gcatggatgt tgacctcggt actcataggc tcctcctgtt atacggcaga
      241 atataccgcc ccggcaaacc gaaaactagt aaagtctgct tcagtttacg cgacgaacgc
      301 tattttataa gcaggtgtga ttcgtcggta tcgctttcta tgtcgataag ctgccacgtg
      361 tggttcttgc cggagttgct gatgccggta cgtatgtaat aggctgcacc gccgttccgg
      421 tacttgtata taaaaaatac cgtatcgttt ttggagccct gcactaaggt acgccgatcg
      481 agcgtggggg tgcctttcag tacctggtcc gcgagccgga agcgggtaga gagggcgtcg
      541 gcggggtact gcttgcggaa tgaagcatcg gcgaagctgc gcgcatcggc tccgttctgc
      601 gtcttgatat cgtgcagggt acgatcgctc agctgcttag cagaagctat ctcctggttc
      661 agcttctgct tgtttgccca gttgagtgcc agggccacga ccaggccgat gcctaatagc
      721 accagtacga gcagtaccgg attgaatcgc ggacggtcgg ctttggaggc cgtgaactgc
      781 tgggctgtat cttccatgca gccacagtag cacaaaaaca gggaaaacac cgccccggct
      841 tgctatactg gtgccatgct agatatccaa ttcatccgtg ataatcccga actggtccaa
      901 cgcaaaagcg aagagaaagg ctaccatgtg tccgtcgccg aactgctcga gctcgatacc
      961 gaacgccgca gcaaactgac gcagatagag gagcttcgct cgcgacgcaa tagcttagcg
     1021 gcggaaatga agaatcagcg gccgactgac gagcagattg ccgccggcaa acagctgaag
     1081 gaagaaatcg ctgcgcgcga agccgagctg cagacaatcg atgacaaata cggtgcacag
     1141 ctgatggccg tgccgaacgt gactttcgat gacgtgccgg tgggcggcga ggaagacagt
     1201 gtcgaaataa aggtggtggg cgagaagccg acgggcgcca aagatcacct ggactatgcg
     1261 actgaacggg gctggctcga tttcgaagcc ggtgccaagg tggccggtac gaaattctac
     1321 tatctgaagg gcgacctggc attgctggaa aacgccatta cccagtacgc cctgaatttc
     1381 tgcgtcgaca aagggttcac ctacatgacg gtgccgcaca tggtaaacag ccgtatcgcc
     1441 accggagccg ggtttgcccc ccgtggtgac aaagaaggca acgaatactt cgtcgaaaat
     1501 gaagatctga cgatgatcgg tacggccgaa gcacctctga ccggctatca tgccgatgag
     1561 atcctggatg agtccgcatt gccgaaacta tacgtcggtt atagcccgtg ctatcgccgc
     1621 gaggccggca cctacggcaa acacacccgc ggactattcc gtgtgcacca gttcaataag
     1681 cttgagatgt atgcctttac gctgcccgag aacagtcagg aaatgcacga acgtatcctg
     1741 gccatcgaag aagaactgtg gcagagcatc ggcgttccgt accacgttat caatatcgcc
     1801 agcggcgact tgggagcacc agcagccaag aagtacgaca tagaatattg gtcgaaggcc
     1861 gacggcgcct accgcgaact gacgagttgc tctaactgta ccgatttcca ggcgcgtaac
     1921 ctcaacatcc gggtgcgccg ccgcgacggc agtatcgaaa ccctgcatac cttgaatggc
     1981 actgccgtat cgctggcgcg ctcgttggtg gtggtgcttg agtacttcca gaatgaagat
     2041 ggcagcctta ccgtaccgga agtgctgcgg ccgtacatgg gcggtaggga gtgcatatag
     2101 ccatgctgac ccacgtcgtc caggccgagg gctatagcgt gagtggcgaa ctgcagaaat
     2161 acacgcagaa aaagctcgac agtcttgagc ggtatatccc gcgctcctgc cgtcgtagcg
     2221 ctcatgtcga agtacggttc aagcaaaagg ccagtaaaga caaaaaccgc aaattcaaca
     2281 cctgtgaact tacactgcat ctgcctggcg acaccctgtt cgccaaagag acgacccagc
     2341 acatgtacgc cgcactggat atcgtggcag tacatatcaa acagcagcta gacgattacc
     2401 gtgccaagca cagtcctcgg tggcgacacc gcctggcacg ccgtttgggc ggtgagtggc
     2461 agtaggcggc acgagcgcgg aagtggctct cgccaacgct ggccaaacag ggtacaatgg
     2521 tacgaaactg aactgacgac aaacggagga gtgaccgaaa gagtatgaac gctgtcttaa
     2581 aaaagctgct cggagacccg caagccaaga ctattaagcg gctgcgccgc agggtagagg
     2641 cggtcaacaa actggcgccg aaatacgaaa agatgtccga tgccgacctg cgcaagcaga
     2701 ccgacgtact ccgtaaacgc ctcaagaaag aatcgctcga tgctatcctg ccagacgcct
     2761 tcgcggtggt gcgcgaagct gccacccgct ctttgggcat gcgccatttc gacgtccagc
     2821 t
//
LOCUS       DASSFD010000053         5524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1569896, whole genome shotgun sequence.
ACCESSION   DASSFD010000053 DASSFD010000000
VERSION     DASSFD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569896"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFH39_01180"
     CDS             <1..290
                     /locus_tag="VFH39_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET6924426.1"
                     /translation="MMSVEKLFFARNVTTAMTVAQARGVVLLCGQQAGMPIFEYTPMQ
                     IKQAITGYGKADKKQMQEMVRVILGLEEIPKPDDAADAIAAALTHAQNAGI"
     gene            complement(287..913)
                     /locus_tag="VFH39_01185"
     CDS             complement(287..913)
                     /locus_tag="VFH39_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924427.1"
                     /translation="MPTFSFMRNRWLLFGAGLVLGALVILVIRFATYSVHNTHYHANF
                     AVYLNGQREEFKGPQYYEEVAACTAHGPIQPAQRAHMHDQINSVIHVHDDGVTWEQFF
                     NNIGWYLGPNFIQTADGTMYTENGNNKLNIMLNGQNLTDLTSIADQTIGDRDRLLISF
                     GDISNAQLQKEYQTVPSTAKHYDETPDPASCAGQLNKVTIGDRFKHLF"
     gene            958..1536
                     /gene="ruvA"
                     /locus_tag="VFH39_01190"
     CDS             958..1536
                     /gene="ruvA"
                     /locus_tag="VFH39_01190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6924428.1"
                     /translation="MIATLSGVVSEKLTDMVVLDVHGVGYGLQVTSEDFERLHAGEPA
                     KLYVYEHVREQAFDLFGFVQPDTKKLFEQLLGVKNVGPKVALAVLDIGTAPAVRGAIA
                     AGDVKLLQSAKGVGKRAAEQIIVELRDKVGLATSEAAEGIVGRPGVNAQDEAVEALIS
                     LGYSPQDATVALKDIDPLLSAEERITLALKQK"
     gene            2122..3159
                     /gene="ruvB"
                     /locus_tag="VFH39_01195"
     CDS             2122..3159
                     /gene="ruvB"
                     /locus_tag="VFH39_01195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6924429.1"
                     /translation="MIERIVNAAAPLADDPVVEEEFEIKLRPHDFANYIGQDRLKQNL
                     QLAIAAAKKRSEALDHVLLYGPPGLGKTTMASVIANEMGAQIRITSGPAIERAGDLAS
                     LLTNLQDGDILFIDEIHRLHRTVEEVLYSAMEDYKLDIMLGKGPSARSLRLDLPKFTL
                     IGATTRTGALAAPLRDRFGHIHRLEFYTPDQIAQIITRAAGILGIKIEPAAAQNLAER
                     ARLTPRIANRLLKRVRDYADVNGDGIIDTPISGKALELLEIDELGLDPADRMLLSAII
                     DSYDGGPVGVETLAALTAEERSTIEDFIEPYLLQIGLLERTPRGRKVTAKAYHHLGKS
                     SVKTTDQKSLL"
     gene            3259..3999
                     /locus_tag="VFH39_01200"
     CDS             3259..3999
                     /locus_tag="VFH39_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6924430.1"
                     /translation="MNPDQNAPQPPQSNENPYNNPLRVMSPGERELGEIRRHPFGLFG
                     VYFGAAVIIILVGVGAALLPSFAPSLSVATRNLAYIIAVFVAIFVLIFTYIAAVIYHG
                     NRWILTSDSITQISQVSLFRKQTSQLSLANLEDVTFDQNGLIPTMFGFGTLKVETAGE
                     RSKFQFPFCPNPSKYARDIIQAHEEFIQSHPEEGVTVRPGSFPQTAYQQPYQQQYQAP
                     PQPEQQYQPPANPVPSEPQQNDEQQQGQ"
     gene            complement(4031..4324)
                     /locus_tag="VFH39_01205"
     CDS             complement(4031..4324)
                     /locus_tag="VFH39_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924431.1"
                     /translation="MKDIQSILDKLRDFTDNVKRYLPVLFVVLLLVIYGFLMMQITAL
                     SNPEPPSGSSTPEATAQVPRINPALVKQLQSLQDNSTNVKALFDQARQNPFQE"
     gene            complement(4321..4965)
                     /locus_tag="VFH39_01210"
     CDS             complement(4321..4965)
                     /locus_tag="VFH39_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924432.1"
                     /translation="MNSKRMYFVMLGVIGLLFIGLFAGTYGINNLLQSHSKQLSDVKA
                     KSESLANQQQGLVKAKKQVAQYSDLEKIAQTIVPQDKDQAEAVREIASLAAASGIPQL
                     SSITFPASTLGAHSSGAVSSGSTAPATPSASSSKSNLTQLVPVPGVQGVYQLQITITQ
                     TNQSKVTYNQFYTFLSKLEQNRRTAQVSSVTLQPDPTNPNYVAFVLIVNEFIKP"
     gene            complement(4967..>5524)
                     /locus_tag="VFH39_01215"
     CDS             complement(4967..>5524)
                     /locus_tag="VFH39_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924433.1"
                     /translation="TYGLRNTSLISWVVALVVAIAGLGVIGTYGLVAIQKSTDTYSQQ
                     VAELQSTLQAQKLAQTEKKVTDISNSFKLVVQVLSKEVLFSQLLKQIATVIPPNASLA
                     NLNISQTTGGVDIVANASDYTTATQVQINLADPNNKIFSKADLVSITCNNGTANARYP
                     CTVQIRALFATKNPFLFINNTTGEK"
BASE COUNT         1197 a         1466 c         1654 g         1207 t
ORIGIN      
        1 aaatgatgtc ggtcgaaaaa ttgttctttg cccgtaacgt caccacggct atgacagtag
       61 cccaggcccg cggcgtggtg ctcttgtgcg gacagcaagc cggaatgccg atatttgaat
      121 acacaccgat gcagatcaag caggccataa ccggctacgg caaggccgac aagaagcaga
      181 tgcaggagat ggtccgggtc attttggggc tggaagagat accgaagccg gacgatgcgg
      241 ctgatgccat cgccgcggcg ctgacgcacg cccagaacgc cggtatttag aacaggtgtt
      301 tgaagcggtc gccgatggtg accttgttga gctggccggc gcagctggcc gggtccggcg
      361 tttcatcgta gtgcttggcg gtcgatggca ccgtctggta ttccttctgt agctgagcgt
      421 tgctgatgtc gccgaagctt atgagcagcc ggtcgcggtc gccgatggtc tggtcggcaa
      481 tcgatgtcag gtcagttagg ttctgcccgt tgagcatgat gttcagcttg ttgttgccgt
      541 tctcggtgta catggtaccg tcagcggtct ggatgaagtt cggaccaagg taccagccga
      601 tgttgttgaa aaactgttcc caggtgacgc cgtcgtcatg cacgtggatg acggagttga
      661 tttgatcgtg catgtgcgcc cgctgcgccg gctggatggg cccgtgagca gtgcaggcag
      721 cgacttcttc gtagtactgc ggacctttga attcctcgcg ctggccgttc aggtagacgg
      781 cgaagttcgc atggtaatgc gtgttgtgca cgctgtaggt cgcaaagcgg attacaagga
      841 tgacgagtgc cccaagcacc agaccggcgc cgaacagtaa ccacctgttc ctcataaaac
      901 tgaacgtagg catgtatcca gtataaccct gcctgccggt gtgccataat agcgggtatg
      961 attgcgacac tttccggggt ggtgagcgaa aagctgactg acatggtggt gctggacgtg
     1021 cacggcgtag gctacgggtt gcaggtgacg agcgaggact tcgagcgact gcatgccggc
     1081 gaaccggcta agctatacgt gtatgaacat gtacgcgagc aggcgttcga tctgttcggc
     1141 tttgtgcagc cggataccaa gaagctgttc gagcagctgc tgggagtgaa gaatgtggga
     1201 ccgaaggtcg ccttggcggt cctggacatc ggcacggcac cggcggtacg cggcgccatt
     1261 gcggccggtg atgtgaagct gctgcagagt gccaagggtg tgggcaagcg ggcggcggaa
     1321 cagatcatcg tcgagctgcg cgataaggtg ggactggcga ctagcgaggc cgccgaaggc
     1381 atcgtcggtc ggccgggcgt gaatgcccag gacgaagccg tggaagccct catctccctc
     1441 ggttattccc cccaggacgc gacggtggcg ctcaaagaca tcgatccgtt gctttccgca
     1501 gaggaacgga ttacactggc gcttaagcag aaataggagt tcctatgacg ttatacggta
     1561 ggccggggta cacgctggaa gtatatcctg atcctggctc gccctcgggc ggtacttata
     1621 gacttcagga tgtttacttg cgaatggaaa cgcatccaga accaggcaac tattcagttt
     1681 tgtggtcaac cgataaagga gccatagatt cgatagacac ggccttgagc gtctttggcg
     1741 aacatgcatt cacaataggc gccgtagcag aaaagggctc ggaagaagaa ggcgatgcat
     1801 atggggaagg agaagtaagg gacgtgattg actacgttag agaagaagcg gacttacgta
     1861 aactttgcta tgtgcgcctc ggtgggccgg ctgtggccac tatttggtcc gctccagcca
     1921 aaagatttgt tgacagtact gccgcgacag cagagcttag tatgatggtg accggcgcgt
     1981 caccgatttc ggtggagcgc atggtttccc agacagctat aagcagcatc gcaagccggt
     2041 accatgaggc gatagcagag ctgagaactg ctgtcccgag cctaccgcaa gcgtaagcac
     2101 atttgcggta taatacattt gatgattgaa cgaatagtta atgcggctgc accactggcg
     2161 gatgacccag tagtggagga agagttcgaa atcaagctgc ggccgcatga ctttgccaat
     2221 tacatcggtc aggatcgctt gaaacagaac ttgcagttgg ctattgctgc cgctaagaag
     2281 cgcagtgaag cgttggacca tgtgttgctg tatgggccac cgggcttagg taagaccacc
     2341 atggcgagcg tgatcgccaa cgaaatgggc gcgcagatcc gcattaccag cgggccggcg
     2401 atcgagcggg caggggactt ggctagcttg ttgaccaacc tgcaagatgg cgacattttg
     2461 ttcatcgacg aaatccaccg gctgcaccgc acggttgaag aagtgctata cagtgccatg
     2521 gaggactaca agctcgacat tatgctcggc aaaggccctt cggcacgcag tttgcggctg
     2581 gatctaccca agttcacgct catcggtgct actactcgta ccggcgctct ggccgcaccg
     2641 ctccgcgacc gtttcgggca catccaccgg ctggaattct atacaccgga tcagattgcc
     2701 cagatcatca cccgggcggc cggcatatta ggcataaaaa tcgagccggc agccgcccaa
     2761 aatctcgccg aacgcgcccg tctgacgccg cgcattgcca accgtttact caagcgggtg
     2821 cgggactatg cggacgtgaa cggtgacggt atcatcgaca cgcccatttc cggtaaggca
     2881 cttgagctgc tggagataga cgagttaggg ctggacccgg ccgaccggat gctgttgagt
     2941 gccattatcg acagctacga cggcggtccg gtgggggtgg agacgctggc ggccctgacg
     3001 gccgaagaac gcagtaccat cgaagacttc atcgaaccgt acctgttgca gatcggcctg
     3061 ctggaacgca ccccgcgggg ccgtaaggtt accgctaagg cgtaccatca ccttggcaag
     3121 tcatcagtta aaacgaccga tcaaaaatcg ttactgtagg gggcatacga cgtgttgcga
     3181 cccaaattcg tccatcatcg gacgccgatg catgcccggt tagctaagcg cccggtagaa
     3241 gggtataatg aggccattat gaatccagac caaaacgctc cccagccacc tcagagtaac
     3301 gaaaatccct acaataaccc tttacgcgtt atgagtcccg gcgagcgcga actgggtgag
     3361 atccgtcggc atccgttcgg tctcttcggt gtgtacttcg gcgcagccgt gatcatcatc
     3421 ctggtcggtg tcggcgctgc cttgctgccg tccttcgcac cgtcgctctc ggtcgctacg
     3481 cgcaacctgg cgtacattat cgccgtattc gtggccatct tcgtcctcat cttcacctac
     3541 atcgcggcag tcatctacca tggcaaccgc tggatattga cctctgatag cattacgcag
     3601 atcagccagg tcagcttgtt ccgcaaacaa accagccagc tgagcctggc caacctggaa
     3661 gatgtgacct tcgaccagaa cgggctcata cctaccatgt tcggtttcgg cacgctcaaa
     3721 gtagagaccg ccggtgaacg ctcgaagttc cagttcccgt tctgtcctaa cccgtccaag
     3781 tacgcccgcg acatcattca ggcgcacgaa gaattcatcc agtcgcaccc cgaagagggg
     3841 gtgacagtcc gccccggtag tttcccacaa accgcttacc agcaaccgta tcagcagcaa
     3901 taccaagcgc ctcctcagcc tgaacagcag taccagccgc cggccaaccc ggtaccctca
     3961 gagccccagc aaaacgacga gcagcagcaa ggacagtaat acccccgcta tgcagcggcc
     4021 ctggctgccc ttattcctgg aaggggttct gccgagcttg gtcgaacaaa gctttgacgt
     4081 ttgtgctgtt gtcctgcagg ctctgcagct gtttcacgag cgccgggttg atgcgcggca
     4141 cctgtgccgt cgcttcgggc gtgctgctgc cgctgggagg ctcaggattg ctcagagcgg
     4201 taatctgcat catcagaaag ccgtagataa caagtagcaa tacgacgaac agcaccggaa
     4261 ggtaccgttt gacgttatcg gtgaagtcac ggagcttgtc gagaatactc tggatatctt
     4321 tcatggtttt atgaactcgt ttacgatcaa tacgaaggcg acatagttcg ggttcgtagg
     4381 gtcgggctgc agggttacgc tgctcacttg ggccgtgcga cggttctgct ccagcttgct
     4441 aaggaaggta taaaactggt tgtacgtgac cttgctctgg ttagtctggg taatggtaat
     4501 ctgtagctgg tagacgccct gtacgccggg tactggtacg agctgggtca ggttcgactt
     4561 actactggat gcagacggag tggccggtgc cgtgctgccc gagctgacag caccggagct
     4621 gtgggcaccg agcgttgaag cggggaaggt gatggagctg agctgcggga taccactagc
     4681 agccgccagg ctggcaatct cacggaccgc ttctgcctgg tccttatctt ggggcacgat
     4741 tgtctgtgcg attttctcga ggtccgagta ctgagccacc tgtttcttgg ctttcaccag
     4801 cccttgttgt tggttggcga gggattcgct cttggctttg acgtcgctca actgtttgga
     4861 gtgggactgc agcaagttat tgatgccata ggtacctgcg aacaaaccga tgaatagcag
     4921 gccgatgaca cccagcatca cgaagtacat gcgcttactg ttcaccttac ttctctcccg
     4981 tagtgttatt gatgaacagg aaggggttct tggtagcgaa cagggcacgg atttgaacgg
     5041 tacatgggta gcgcgcgtta gcggtgccgt tattgcaggt tatgcttacc agatcggcct
     5101 tgctgaagat cttgttgttc ggatctgcca aattgatttg tacctgcgta gcagtcgtat
     5161 aatcgctggc attggcaacg atatcaacgc caccggtagt ctgactgatg ttcaggttcg
     5221 ccagacttgc attcggggga atcacggtag cgatctgttt cagaagctgc gagaacagaa
     5281 cttctttgga aagcacctgc acgaccagct tgaagctatt ggatatgtcg gtcacctttt
     5341 tttcggtctg cgccagtttt tgggcctgta gcgtcgattg caactcggcc acttgttggc
     5401 tgtaagtgtc ggtcgatttc tgaatggcga cgaggccata ggtaccgatg acacccaggc
     5461 cggcaatagc cacgaccagg gcgaccaccc agctgatcag gctggtgttg cgcagaccgt
     5521 aggt
//
LOCUS       DASSFD010000054         4875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_905204, whole genome shotgun sequence.
ACCESSION   DASSFD010000054 DASSFD010000000
VERSION     DASSFD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905204"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VFH39_01220"
     CDS             complement(<1..898)
                     /locus_tag="VFH39_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6924434.1"
                     /translation="MSRSQPGTRPMYAAPSRPAASQLPPAAQQPPVPVITPVNAPKPA
                     PITQLAAASKTSIDGFKPAGKPANRLAGPGKMKLNIPGAVLNEAGIAPPLPLEPAAQP
                     FQATTTDDDRGSLPVTPVDLNLEDTPAAARPKNRLHAAKAFRKWMLRGGLAFAVMLLL
                     IGGLLFYQGLSKVHKVFKGSANRPAALQANVDPQLLKGEGDGRVNILLLGNGGNGHDG
                     PDLTDTIMVASIDPVNKTADLLSVPRDLWVKMPNGFMGASQKINAAYEDGKYTYLHRQ
                     DASNANYKAVEAGFASADKVVSQ"
     gene            1179..1877
                     /gene="lepB"
                     /locus_tag="VFH39_01225"
     CDS             1179..1877
                     /gene="lepB"
                     /locus_tag="VFH39_01225"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6924435.1"
                     /translation="MQPTNSSTPSPVPSSSGGPKASGPINMDIPPAPEPDHSKGTSAL
                     REFLSTVGVLVAALLVALGLIAFVFQSYQVDGPSMQQTLHNNDRLIVWKLPRTWARIT
                     GHQYVPKRGDILIFTESGLSAYGQTDTKQLIKRVIGLPGDRVVVKNNVITIYNKQFPN
                     GFQPDKTLPYHNQITEPATQDVDVTLAKDQLFMCGDNRTNSLDSRFFGPINTNQVIGK
                     LVLRVLPLSDAERF"
     gene            1924..2991
                     /locus_tag="VFH39_01230"
     CDS             1924..2991
                     /locus_tag="VFH39_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924436.1"
                     /translation="MYESDPWQLTDAVSAQLDIPLDKSTLYAREDGHIAGTSIGGEHD
                     VHMRRLDWAEAVSATPFGTFRLTEKFHWNGLLRHQRGGIRLELPDTASHTRQAICLAE
                     VISAPAGINAAQRLLGREPGSDAFMLKRYKGKAKYTSGEWVEGFAQQVVPIADLSNPD
                     YALHDTYPTDHVQSWINTPPEICRVIAERAAHLQAKYGDRIHAEHNRPRSVNDFVNAV
                     DLLSNFTRVLFQRTMNTVDIVRPGQLIEKFERVASDNPLSYAQLRRRYCWPKGDDWYD
                     DEGITTLMHLGLLRPAFSRSENLQRFNAVRSTFIAHIARMCITAEEVAPNAEFSPVER
                     AFTLDEWHAVAQHFGDAKISV"
     gene            complement(2988..3854)
                     /locus_tag="VFH39_01235"
     CDS             complement(2988..3854)
                     /locus_tag="VFH39_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET6924437.1"
                     /translation="MASSKQRGLGTSFSTLIPQGLDVAALAEDGERVQKLALDVLEPN
                     PDQPRQHFDPSALDELAQSIKQYGVVQPLVASPDGKKFRIIAGERRWRAARLAGLKQV
                     PVIVRTSKELEQLEIAIIENVQRVDLSPLEQAVSIERLHSQFNLSYEEIAKRLGKANS
                     TVVNIVRLLQLPDDAKEALLQKQITEGHARQILALKKLPEKQAELLELILKHSWNVRQ
                     AERYVTSHKEGVTETEQAHERVQTETPATKALSKRLGTKVAVKRMAHGGRLEITFKDD
                     EHLQKLLESLDN"
     gene            complement(3949..4740)
                     /locus_tag="VFH39_01240"
     CDS             complement(3949..4740)
                     /locus_tag="VFH39_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924438.1"
                     /translation="MIDWFVGGLVVFALLLMAYWFGPQKNRSGTIIIRAHRYAFEAGN
                     TLSYSGRQFAGLSIELPKKLPHMYLDHRVASRFSGPVRTYANDQQVDLEGDFDRYFQL
                     FTPKGNEALALSIMSPDVMQTLMQAAYSYDIEIKADRVNILAGHYAGRLQDEQIAEME
                     KAARAVLSEIDQKLPTWSADDQAQAVATELKYYDEQSVRLFGRYVGIGVLAIAVSILV
                     ATPLFYEAHRLAAAGSDAVVLYVTLGFVAFPIFPILIIYAERTDT"
BASE COUNT          971 a         1538 c         1309 g         1057 t
ORIGIN      
        1 tctggctcac caccttgtcg gccgatgcaa agccggcctc gaccgctttg tagttggcat
       61 tgctggcatc ctgccggtgc agataggtgt atttgccgtc ctcgtaggcg gcgttgatct
      121 tctgactggc gcccatgaag ccgttcggca tctttaccca gaggtcacgg gggacgctca
      181 gcaagtcggc cgtcttgttg accgggtcaa tgctcgccac catgatagtg tcggtcaggt
      241 ccgggccgtc gtggccgtta ccgccattgc cgagcagcaa gatgttcacc cggccgtcgc
      301 cctcaccctt cagtaactgc ggatcgacgt tcgcctgcag agctgcggga cggttggcgc
      361 tgcccttgaa caccttatgg accttactca agccttgata aaacagtagc ccaccaatca
      421 gcagcagcat gactgcgaag gccaggccgc cacgtagcat ccacttgcgg aaggctttgg
      481 cagcatgcag acggttcttg ggccgggcgg ccgccggcgt atcttccaga ttgaggtcga
      541 ccggtgttac cgggaggctg cccctgtcat catcggtggt cgtcgcctga aacggttgcg
      601 ctgccggttc caacgggagc ggcggcgcga tgccggcctc gttcaggacc gcaccgggga
      661 tattcagctt catcttgccg gggccggcca ggcggttcgc cggcttaccg gccggcttga
      721 aaccgtcgat gctggtcttc gatgcggcag ccaattgcgt aatgggggcc ggttttggag
      781 cgtttacagg ggtgataacg ggcaccggtg gctgctgggc tgccggtggc agttgactgg
      841 ctgccggacg cgaaggagcg gcgtacatcg gccgcgtccc cggctgggaa cggctcatag
      901 accggggctg taccgccggc tgtcgtgcga ctgatcttac gaatccatcg actgtttgtg
      961 tttggcggga ccggtccggc ctcactaccc gaaaattatc cataccagta agcacaagta
     1021 taacctgccg cctgcttatg gtaaaccttc ggagcgttgg ttaatgcaca accggggtgt
     1081 gggcaaaacc ctacggagcg acttttacag cggtcagatt gcccaaaacc gtaaatccta
     1141 caggttttac acaccgtttc tgctatagtt aatggtcgat gcaacctact aattcttcta
     1201 cgccctcacc ggtgcctagt agctcagggg gtcctaaggc cagcggtccg atcaacatgg
     1261 atatcccgcc ggcacccgaa cccgaccatt ccaaaggtac cagcgccctg cgtgaattcc
     1321 tgtcgaccgt cggcgtgctc gtcgccgccc tgttggtagc gctcggtctt atcgccttcg
     1381 ttttccagtc ctaccaggtc gacggcccga gcatgcagca gaccctgcac aacaacgacc
     1441 gcctgatagt atggaagctg ccccgtacct gggcccgcat caccggtcac cagtacgtcc
     1501 ccaagcgcgg cgatatcctt atcttcaccg aatccggcct ctccgcctat ggccagaccg
     1561 acaccaagca gcttataaag cgcgtcatcg gtctgcccgg cgaccgcgtc gtggtcaaga
     1621 acaacgtcat caccatctat aacaagcaat tcccgaacgg tttccagccc gacaagacgc
     1681 tgccctacca caatcaaatc accgaacccg ccacccagga cgtagacgtc accctggcca
     1741 aagaccagct gttcatgtgc ggcgacaacc gcaccaactc gctcgatagc cgcttcttcg
     1801 gacccatcaa caccaaccag gtcatcggca aactagttct ccgagtcctc cccctctccg
     1861 acgccgaacg gttctaggca atttttgccg taaaaagcat attatggtat cgtaacccta
     1921 atcatgtacg aatccgatcc ctggcagctc accgacgctg tctctgccca actagatata
     1981 ccgctcgata aaagtaccct gtatgctcgt gaagacggcc acatagcggg caccagtatc
     2041 ggcggtgaac acgatgtgca catgcgccgc ttggattggg cagaagcagt gtctgcaaca
     2101 ccttttggaa cctttaggct gaccgagaaa ttccactgga acggtctgtt gcgacaccaa
     2161 cggggtggta taagactcga gttaccggac accgcttccc atacacgaca agcaatctgc
     2221 ctcgctgaag tcatttccgc accagcgggc attaacgccg cgcaacgcct tcttggccgt
     2281 gagcccggca gcgatgcttt catgcttaaa cgctataagg gtaaggccaa atacacctca
     2341 ggcgagtggg tcgaaggctt tgcccagcaa gtagtgccga tagccgacct cagcaaccct
     2401 gactacgccc tccacgacac ctaccctacc gatcatgtgc aaagttggat taacactcct
     2461 ccggaaattt gccgcgtaat tgcggagcgt gcagctcact tacaagccaa gtacggtgac
     2521 cgaattcatg cggaacataa ccgtccacgc agcgtgaatg acttcgtgaa tgctgtcgac
     2581 ctcttaagca attttacccg tgtgctgttc cagcgcacca tgaacaccgt agatatagtc
     2641 cgtccgggac agttgattga aaagttcgaa agagtcgcga gcgacaaccc cctttcgtat
     2701 gctcagctgc gtcggcgcta ctgctggcca aagggagatg actggtacga cgacgagggc
     2761 atcacgacac tcatgcacct cggcttactc cgaccggcat tctcacgatc agaaaacctc
     2821 cagcggttca acgccgtccg ctcaacattc attgctcaca tagcccgcat gtgcattacg
     2881 gccgaagagg tcgcgccgaa cgcagagttc agcccagttg agcgcgcctt cacgttggac
     2941 gagtggcacg ccgttgccca gcacttcggc gacgcaaaaa tatcagtcta gttgtcgaga
     3001 ctctcgagca gtttctgcaa atgctcgtcg tctttaaagg tgatttccag ccggccgccg
     3061 tgggccatgc gcttcaccgc cacctttgtg ccgaggcgtt tgctcagggc tttggtggcc
     3121 ggcgtttcgg tctgtacccg ttcgtgcgcc tgttcggtct cggtcacgcc ttctttgtgg
     3181 ctggtcacgt agcgttccgc ctgacggacg ttccagctgt gcttcaggat aagttcgagc
     3241 agttccgcct gcttttcggg cagcttcttc agcgccagaa tctgccgggc gtgcccttcg
     3301 gtaatctgtt tttgcagcag ggcctctttg gcatcatccg gcagttgcag cagccgcacg
     3361 atgttcacca ccgtcgagtt cgccttgccg agccgcttgg caatttcttc gtaactgagg
     3421 ttgaactggc tgtgcagccg ctcgatgctc accgcctgct cgagcgggct caggtccacc
     3481 cgctgcacgt tttcgatgat cgcgatctct aactgttcaa gttctttact cgtccggacg
     3541 atgaccggca cctgtttgag gccggccagc cgggccgccc gccaacgccg ctcaccagcg
     3601 atgatccgga acttcttgcc gtccggcgat gccaccagcg gctgcaccac gccgtattgc
     3661 tttatggatt gcgccagttc atccagcgcc gacggatcga aatgctgccg cggctggtcc
     3721 gggttcggtt ccagcacgtc cagtgctaac ttctgtaccc gctcgccgtc ttcggccagc
     3781 gccgccacgt ccaacccctg aggaatcagc gtggagaaac tcgtccccaa ccctctttgt
     3841 ttgctgctcg ccataatgct tttattttag caaaccatcg gccttccgtg aaaaccggtt
     3901 ttcacagctg tccttgccaa gaagtgaacc ggctcacgac ggcttcgctt aggtgtcggt
     3961 ccgttccgca tatatgataa gtatcggaaa gatcggaaac gccacgaagc cgagcgtaac
     4021 atacagcacc accgcatcgg agccggccgc ggccagccgg tgcgcctcat agaatagcgg
     4081 tgtcgccacg agtatactga ccgcgattgc cagtacccct atgccgacgt accgtccgaa
     4141 cagccggacc gactgctcgt cgtaatattt gagctccgtg gccaccgctt gcgcctggtc
     4201 atccgccgac caggtgggta acttttggtc gatttcactg agcacggccc gggccgcctt
     4261 ttccatctcg gctatctgtt catcctgcag ccggccggcg taatgccccg ccaggatgtt
     4321 gacccggtcc gctttgatct cgatatcgta gctatacgcc gcttgcatca aggtctgcat
     4381 gacatcgggc gacataatac tcagcgccag cgcctcatta cccttcggtg tgaacaactg
     4441 gaaatagcgg tcaaagtctc cttccagatc gacctgctgg tcattggcat acgtccgcac
     4501 cggtccgctg aacctcgaag cgacccggtg atcgagatac atatgcggca acttcttcgg
     4561 taattcgata ctcagcccgg caaactgtcg gccactatag ctcaaagtgt tgccggcttc
     4621 gaacgcgtac cgatgagcgc ggattataat cgtgccgctg cggttctttt gcggaccgaa
     4681 ccaatacgcc atcagcagta gcgcgaagac tacgaggccg ccgacgaacc agtcaatcat
     4741 accggcagta tatcagttaa ccgagccgtt tgctgacttc gtccgccaga tgtttgtagg
     4801 cgcgggcgcc cttgctccac ttgtcgtgct cctggatggt ccgcccgaag ctcggcgcct
     4861 cggccaggcg cacgt
//
LOCUS       DASSFD010000055         7813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_916688, whole genome shotgun sequence.
ACCESSION   DASSFD010000055 DASSFD010000000
VERSION     DASSFD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916688"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..663
                     /gene="mscL"
                     /locus_tag="VFH39_01245"
     CDS             244..663
                     /gene="mscL"
                     /locus_tag="VFH39_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET6924439.1"
                     /translation="MFKEFRKFIMRGNVVDLAVAFVVGAAFNAVVQGIVKDLLTPLIA
                     AIGGQPDFSKLTFKVHNSTFLYGDVLNLIISFLVIAAVVFFFVVQPINKLQTRAKRNK
                     TPEEPTDKKCPECLSTIPKAATRCAYCTTKLTAAKAS"
     gene            727..1413
                     /locus_tag="VFH39_01250"
     CDS             727..1413
                     /locus_tag="VFH39_01250"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6924440.1"
                     /translation="MVDAIYNGRSARILYSADRLTAQSGLALDGQPELLFDYNERFME
                     LVRGLKPKRVLLLGGGAYTLPRAIIDEFPSVDVTVIELDEGLFAIGQEYFGVPYTKRL
                     RVVHGDAVKELPEISAVFDLIIIDVFIGPLIPSGFQTADFVHELQKHLAMQGTVAMNI
                     IAGYYGQRSMSLRRQIASFESAFSNVQLFPAGAETSLWTPQNFILTASNSTTDLQPFQ
                     RYKALELLTA"
     gene            complement(1410..1838)
                     /gene="atpC"
                     /locus_tag="VFH39_01255"
     CDS             complement(1410..1838)
                     /gene="atpC"
                     /locus_tag="VFH39_01255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET6924441.1"
                     /translation="MIHFQLVALSGTKFDDDVYEVILPTLDGEIGVLEDHMPLVSVAT
                     NGAIAVRRNAKDTDREREYFATNGGVIEVSDNTLRVLVDEADHADDINEAEAEAAMER
                     AKSMQAEAKDQVSLEHAQQLVDRQAVRLQVAGLKRRHQKH"
     gene            complement(1840..3237)
                     /gene="atpD"
                     /locus_tag="VFH39_01260"
     CDS             complement(1840..3237)
                     /gene="atpD"
                     /locus_tag="VFH39_01260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6924442.1"
                     /translation="MATATKEKIRTGKITQIVGVVVDVEFATDNLPSIYNALKVQNGD
                     QELTLEVAQHLSESSVRAISLGGTDGLERGAEVTDTGAPISVPVGEDTLGRMFNVTGE
                     PIDGKANKFSQKAPIHRAPPPLIDQSPTVEILETGIKVIDLICPMTKGGKAGLFGGAG
                     VGKTVLIQELINNIAKFHSGYSVFAGVGERTREGNDLYGEMEESGVIKNTALVFGQMN
                     EPPGARLRVALSGLTIAEGFRDKGSDVLLFVDNIFRFTQAGAEVSALLGRLPSAVGYQ
                     PNLAQEMGQLQERITSTREGSITSVQAVYVPADDMTDPAPATTFAHLDSTITLSRALT
                     EIGIYPAVDPLDSTSTILDPEIVGEDHYNVAREVQRVLQRYKDLQDIIAILGMEELSE
                     EDKQLVSRARRIQRFLSQPFFVAEQFTNNPGQYVKLSDTIRSFQEILDGKHDDKPESA
                     FYLKGSIDDVVKAAK"
     gene            complement(3502..4371)
                     /gene="atpG"
                     /locus_tag="VFH39_01265"
     CDS             complement(3502..4371)
                     /gene="atpG"
                     /locus_tag="VFH39_01265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET6924443.1"
                     /translation="MASTIVLRRRITSIKNTRQITQAQQLVAASKLRRAQEYATQSRA
                     YGELANELLAKLSGLAEVERQPLFEKRAVKNRLYILITSNSGLAGAYNANVIKQLTNG
                     VKRDRDAKVSSHVIAIGNKGAQFVRRISDVDLQAAYPGFGDHPTANDLRPVLNTIVEQ
                     YKAGEVDEVHVLYTQFKSTVTQVAQDLQLLPAQAPTDGEVVEGQVSNFEPDVETVIDQ
                     VATRLIEAQVWQALLESIASEHAMRMVAMKNATDNAKDLIDDYTLELNTARQAAITQE
                     LAEITGGAEALNG"
     gene            complement(4364..4663)
                     /locus_tag="VFH39_01270"
     CDS             complement(4364..4663)
                     /locus_tag="VFH39_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924444.1"
                     /translation="MSSTIFGPSEHWRGDFAVIFDDALGVQNAPEKPGATDQFIWASG
                     RMIDLVEGILSGALGDEELGDSEWDAAYARVLKSTEMGYQYGKLAASALVGVNHG"
     gene            complement(4656..4922)
                     /locus_tag="VFH39_01275"
     CDS             complement(4656..4922)
                     /locus_tag="VFH39_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924445.1"
                     /translation="MVNTNPGSKWPVHPRERFSDAELGLSESDVASYDALTSDALNQI
                     IGCATQELLHGSPIGVKRIAAIDTLHALASKLGFELVPVERIDV"
     gene            complement(4928..6469)
                     /gene="atpA"
                     /locus_tag="VFH39_01280"
     CDS             complement(4928..6469)
                     /gene="atpA"
                     /locus_tag="VFH39_01280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET6924446.1"
                     /translation="MAELSITELSADIRAAIAELKERPEIESVGVVTRVGDGIAWVYG
                     LSGAGFNEMLEIDGISGEKITAFAFNLAEDEIGAVILGDDTQVKAGATVRLSGQVLEV
                     PVGPELVGRVVDPLGNPLDGGEPIKTKQTSRVERPAPGVLDRKSVHEPLMTGLVAIDA
                     MVPIGRGQRELIIGDRQTGKTAIAVDTMVNQARQKTGVINVYVAIGQKLSKIAALQER
                     LKREGVMDQTIIVATGPSDPAAMLYLAPYAGAAMGEYFRDNKQHALIIYDDLTKHAQA
                     YRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDELGAGSLTALPIIETQAGDIS
                     AYIPTNVISITDGQIFLETNLFYQGIRPAISVGLSVSRVGGSAQTKAIKAAGGSLKLA
                     LAQFRELAAFSQFSTDLDAETRHTIERGQRLTELLKQPQFAPYAIWEMYSVLYAAQQG
                     AFDIVPLEKIDAAREALLRELKSKHAKLTETLNTGDKPSDEQNEQVLKVAKDIAKQYE
                     VKANK"
     gene            complement(6473..6877)
                     /locus_tag="VFH39_01285"
     CDS             complement(6473..6877)
                     /locus_tag="VFH39_01285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET6924447.1"
                     /translation="MKAPRTKLAAIVADRTLAGGNARTLSRELAAYLLSEGRADELES
                     ILRDVQADWAEAGYVEVLARSAYPLTSENRAEIQQQVSRLYPSAKQIVVTEVRDPEVI
                     GGVQIRLSDRQLDLSVEHKLNRFKQLTTNGKE"
     gene            complement(6882..7415)
                     /gene="atpF"
                     /locus_tag="VFH39_01290"
     CDS             complement(6882..7415)
                     /gene="atpF"
                     /locus_tag="VFH39_01290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET6924448.1"
                     /translation="MISSVTQFAESSSGGIGALGLDGKALIIQLVSFLLAFWVLERFA
                     FKPIIRMMDERRKTIEEGVTLGEQMKKDQAALEAEVEKTLHEARQEADGIIASAQETS
                     RQTVREAEDKAREKAEGIVAEAQDRIESDRKRVRKQLEAEVVSLISDATEAIIGEKVD
                     AKKDAQLIDRALKEQKA"
     gene            complement(7561..>7813)
                     /locus_tag="VFH39_01295"
     CDS             complement(7561..>7813)
                     /locus_tag="VFH39_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924449.1"
                     /translation="VPLAAHLAATVTQPEATQTLAHGIMIGAGFIGPTIGIGLIGANY
                     LAAVGRNPEASRFLGQALVFVGLVEVYGLLAFAATFFVK"
BASE COUNT         1478 a         2325 c         2224 g         1786 t
ORIGIN      
        1 aacccggaac tggcaacctg gatgcgcttg gaagcgtcac gccagtatcc ggatgacttg
       61 ccggctcgcg aaaagacgat tgagcttgcc ctggccgtgc tcacgatggt gcgcatcgag
      121 cagttcaaag ccgacttgca aaagacagca tcccgcccac ggaagccctg attgcaaagg
      181 acctgaactt gcgaaccatc ggcttcgttg tatgatggaa ccatagtaac ggagacctca
      241 gttatgttca aagaattccg gaaatttatc atgcgtggca atgtggtcga cctggccgtc
      301 gccttcgtgg tcggggccgc gttcaatgcc gtggtgcagg gcatcgtcaa agacctgctg
      361 acgccactca tcgctgccat cggcggtcaa cccgattttt ccaaactgac gttcaaagta
      421 cacaacagca cgtttctgta tggcgatgtc ctaaatctga tcatttcttt cctggttatc
      481 gctgcggtag ttttcttctt tgtagtacaa ccgatcaaca aactgcaaac ccgtgccaag
      541 cgcaacaaaa cgcccgaaga acccaccgac aaaaaatgcc ccgaatgcct cagtaccatc
      601 cccaaagccg ccacgcgttg cgcctactgc acgaccaagc tcacggccgc gaaagccagc
      661 taaaccgttt tgcgcaaaca ttacaatctc aataagaaag tcacgaccgc agctggaagc
      721 taccatgtgg tcgacgccat ttacaacggc cgcagcgccc gcatcttgta tagcgccgac
      781 cggctgaccg cgcaatcggg gctggccctc gatggccagc ctgaactgct gttcgactac
      841 aacgaacggt ttatggaact ggtgcgtggt ctgaagccga agcgtgtact gctgctgggt
      901 ggcggggcat acacattgcc ccgggcgatc atcgacgagt ttccgtccgt cgatgtgact
      961 gtcattgaac tggacgaagg attgttcgcc atcggccagg agtatttcgg tgtgccgtac
     1021 acgaagcggc tacgcgtcgt ccatggtgat gccgtaaaag aactgccgga aatcagcgct
     1081 gtttttgatc tgataatcat cgacgtcttc atcggaccgc tgattccgtc cggcttccag
     1141 accgctgact tcgtgcatga actacagaaa cacctggcca tgcagggaac ggtcgccatg
     1201 aacatcattg ccggctatta cggccagcgc tcgatgtcct tgcggcggca gatcgccagt
     1261 ttcgaatctg ccttcagcaa cgttcagctg tttcccgccg gcgccgagac atcgctctgg
     1321 acaccgcaga attttatcct cacggcaagc aatagcacta ccgacctgca gccgttccaa
     1381 cgctacaaag ccttggagtt actgacggct tagtgctttt ggtggcgacg cttcaggcca
     1441 gcgacttgca gccggaccgc ctgccggtcg accagctgct gagcgtgttc caggctcacc
     1501 tggtccttgg cttcggcctg catcgatttg gcccgctcca tggcggcttc ggcttcggct
     1561 tcgttgatgt cgtctgcatg gtcggcttcg tcgaccaaca cccgcaacgt attgtcactg
     1621 acttcgatga cgccgccgtt ggtggcgaag tattcgcgct cccgatcggt atccttggcg
     1681 ttgcgccgga cggcaatcgc accgttggtg gcaacgctta ccagcggcat atggtcttcg
     1741 agcacgccga tctcaccgtc cagcgtcggc aggatgactt cgtagacgtc atcgtcgaac
     1801 ttcgtgccgc tcagggcgac cagctggaaa tggatcacgc tacttggccg cctttacgac
     1861 gtcgtcgatg ctgcccttca ggtagaaggc gctttccggc ttgtcatcgt gcttgccgtc
     1921 caatatctcc tggaaggagc ggatcgtgtc gctcagcttc acgtactggc cagggttgtt
     1981 ggtgaactgt tcggccacga agaagggctg gctcaggaag cgttggatgc ggcgggcgcg
     2041 ggacaccagc tgcttgtctt cctcggaaag ttcttccata cccaagatgg cgatgatgtc
     2101 ctgcaggtcc ttgtagcgct gcagcacccg ctggacttcg cgggcgacgt tgtagtggtc
     2161 ctcaccgacg atttcggggt caaggatggt actggtactg tccagcgggt cgacggccgg
     2221 gtaaatacca atttccgtca gcgcacggct cagcgtaatg gtgctgtcca agtgggcaaa
     2281 ggtcgtcgcc ggggctgggt cggtcatgtc gtcggcaggc acgtacacgg cctgcacgga
     2341 ggtgatggag ccttcacggg tcgaggtaat tcgctcctgc agctggccca tttcctgcgc
     2401 caggttcggc tggtaaccga cggccgaagg cagacgaccc aacagggctg acacttcggc
     2461 gccggcctgg gtaaagcgga agatgttgtc gacgaagagc agcacgtcgc tacccttgtc
     2521 acggaagcct tcggcgatgg tcaggccgct cagcgccacc cgcagacggg cccctggtgg
     2581 ttcgttcatc tgcccgaaga ccagggcagt gttctttata acgcccgatt cttccatttc
     2641 gccgtacagg tcattacctt cacgggtacg ctcgccgacg ccggcgaaca cactgtaccc
     2701 gctgtggaac ttggcgatgt tgttgatcag ctcctggatc agcacggtct taccgacgcc
     2761 ggcaccaccg aacagaccgg ctttaccacc cttggtcatc ggacaaatga ggtcgatgac
     2821 cttgatgccc gtttccaata tctcgaccgt cggtgactgg tcaatcagcg ggggcggtgc
     2881 gcggtggatc ggcgccttct ggctgaactt gttcgctttg ccgtcgatcg gctcgccggt
     2941 aacgttgaac atgcggccca gcgtatcttc accgaccggc accgatatgg gcgcaccggt
     3001 gtcggtgact tcggcaccac gttccagccc gtcggtaccg ccgagcgaaa tcgctcgcac
     3061 gctggactcg ctgaggtgtt gcgcgacctc caatgtcaac tcctgatcgc cgttctgcac
     3121 cttcagtgca ttataaatcg acggaaggtt gtcggtcgcg aactccacgt ccaccaccac
     3181 acccacgatc tgggtgatct taccggtcct tattttttcc ttcgttgctg ttgccattat
     3241 agttctcctt cttccatttg tcgtcgcagc tcctgtcttt gcagtaaacg tcttgctagt
     3301 cccgagctca atggggatac gagtagctcg ccgtcaccga catcatccgg ccggctgcgc
     3361 attatgtctg actctggcct accttgatga tcaaaatctt caggcatcac atatgtttcc
     3421 tgcccgtcgc aatccacgac aggaattcca gcctgcgggt cgtctggcga ttcgatatgt
     3481 aatcgattgc cgacataata attagccatt gagtgcctcc gcgccaccgg ttatctcggc
     3541 cagttcctga gtgatggcgg cctgacgggc cgtgttcagt tccagcgtgt agtcatctat
     3601 caggtctttg gcattgtcgg tggcgttctt catagcgacc atgcgcatag cgtgctcgga
     3661 agcgatggat tccagcagcg cttgccacac ctgggcttca atgagcctgg tggcgacctg
     3721 gtcaatcact gtttccacgt ccggctcaaa gttacttacc tggccttcga cgacttcgcc
     3781 gtcggtcggc gcttgcgccg gcaacagctg caaatcttgc gctacttgcg tgacggtcga
     3841 tttgaattgg gtgtacagca cgtgcacttc gtcgacttcg ccagccttgt actgctcgac
     3901 aatggtgttg agcacgggac ggaggtcgtt ggctgtcgga tggtcgccga agccggggta
     3961 ggcggcctgc aggtcgacgt cgctgatgcg gcggacgaac tgcgcgccct tgttaccgat
     4021 ggcgatcaca tggctgctca cctttgcgtc gcggtcccgc ttgacgccgt tcgtcagctg
     4081 tttgatgacg ttggcgttat atgccccggc caggccggag ttgctggtta tgaggatgta
     4141 caaacggttc ttaaccgcac gcttctcaaa cagcggctgg cgttcaactt cggccaggcc
     4201 gctcagcttg gccaacagtt catttgccag ttcgccatat gcccggctct gcgtggcata
     4261 ctcttgcgca cgccgcagct tgctggcggc aaccaactgc tgcgcctgcg taatctgccg
     4321 cgtatttttg atgctggtga tgcgtcgccg aagaacaatc gtactagcca tggttcactc
     4381 ccaccaaagc ggaagcagct agcttgccat attgatatcc catctccgta cttttgagta
     4441 ctctggcgta ggctgcatcc cattctgaat caccaagctc ttcgtctcct aaggctccgc
     4501 ttaaaatgcc ttcgaccaaa tctatcattc ttccggatgc ccagatgaat tgatctgtcg
     4561 cacctggttt ttcgggcgca ttttgaacac caagtgcatc atcaaaaatg acagcaaagt
     4621 caccgcgcca gtgttcggac ggaccaaata tcgtactaga catctatccg ctccacggga
     4681 actaattcaa accctaactt agacgccagc gcgtgaagcg tatcaattgc cgcaatccgc
     4741 ttaaccccta tggggctgcc gtgtagtaat tcctgcgtcg cacaaccaat aatttgattt
     4801 aaggcgtcgc tggtaagtgc atcatagctt gcaacatcgg attcacttaa acccagctca
     4861 gcgtcactga accgctctcg cgggtgcact ggccatttag aacctgggtt tgtatttacc
     4921 atttctatta cttgttagcc tttacttcgt attgcttggc gatgtctttg gcgaccttca
     4981 ggacttgctc gttctgttcg tcgctcggct tgtcgccggt gttcagggtt tcggtcagct
     5041 tagcgtgctt gctcttcagc tcgcgcagca gggcttcgcg ggcggcgtcg atcttctcta
     5101 acggtacgat gtcgaaggca ccttgctggg cggcgtacag cacgctgtac atttcccata
     5161 tggcgtacgg cgcgaactgc ggctgcttga gcagctctgt cagacgctgg ccgcgctcga
     5221 tggtgtgccg ggtctcggcg tcgaggtcgg tagagaactg actgaaggca gccagttcgc
     5281 ggaactgggc cagagccagc ttcaggctac caccggcggc cttgatggcc ttggtctggg
     5341 cggaaccacc cacacgggag accgaaaggc cgaccgagat agccgggcgg atgccctggt
     5401 agaacaggtt ggtctcgagg aagatctgac cgtcggtaat cgaaatgacg ttggtcggaa
     5461 tataggcgga aatgtcaccg gcctgcgtct cgatgatcgg cagggcagtg agggaaccgg
     5521 caccgagctc atcgctcagt ttggcggcgc gctccagcaa acgggagtgc aggtagaaga
     5581 catcacccgg gtaggcttcg cgtcctggcg gacggcgcag cagcagggac atctggcggt
     5641 acgcttgggc gtgctttgtc aggtcatcgt agatgatgag cgcgtgctgc ttgttgtcgc
     5701 ggaagtactc acccatagct gcacctgcgt atggtgccag gtacagcatg gctgccgggt
     5761 cgcttggacc ggtggcaacg atgatggtct gatccataac gccctcacgc ttcagacgct
     5821 cctgcagggc agcgatctta ctcaacttct gaccgatggc cacgtagacg ttgatgacgc
     5881 cagtcttctg gcgcgcctgg ttcaccatcg tatcgacggc aatggccgtc ttaccggttt
     5941 gacggtcgcc gatgatcagc tcacgctggc cgcggccgat cggcaccatg gcatcgatgg
     6001 cgacgaggcc ggtcatcagg ggttcgtgca cgctcttgcg gtccagcacg ccgggggcgg
     6061 ggcgctccac gcggctggtc tgcttggtct tgatgggttc gccgccatcc agcgggttgc
     6121 ccaaggggtc gaccacgcgg ccgaccagtt ccggaccgac cgggacctcc agcacctgtc
     6181 cgctcaaacg gacggtggca ccggccttca cctgggtgtc atcacctaag ataacggccc
     6241 cgatctcatc ctctgccagg ttgaaggcaa aggcggtgat cttctcaccg ctgataccgt
     6301 cgatctccag catttcgttg aagccggcac cgctcaggcc atagacccag gcaatgccgt
     6361 caccgacgcg ggtaaccacg ccgacgcttt cgatttccgg gcgctccttc agttcagcga
     6421 tggctgcccg gatgtccgcc gaaagttcgg ttattgataa ttcagccatt cgttattcct
     6481 ttccgttcgt cgtcaattgt ttaaatcggt tcaatttgtg ctccacgctg aggtccagct
     6541 ggcggtcgga caacctgatc tgcacgcccc cgatcacttc cggatcacgc acttcggtca
     6601 ccacgatctg cttggcagac gggtacaacc ggctgacctg ctgctgaatc tcggccctgt
     6661 tctcgcttgt cagtggatag gcgctgcggg ccaggacttc cacgtaaccg gcctcggccc
     6721 agtcggcctg cacatcgcgc aggatcgact cgagctcgtc ggcccggcct tcgctcagca
     6781 ggtacgcggc cagttcgcgg ctcaacgtcc tggcgttccc accagcaaga gtgcggtcgg
     6841 cgacgattgc agccagtttg gtacggggag ctttcatgct tttaggcttt ctgctccttc
     6901 agcgcgcggt cgatcaactg cgcgtccttc ttggcatcga ctttctcgcc gatgatggct
     6961 tcggtagcgt cacttatcag gctgaccacc tcggcttcca gttgcttgcg gacgcgcttg
     7021 cgatcggatt cgatgcggtc ctgtgcctca gccacgatgc cttcggcctt ctcgcgagcc
     7081 ttgtcctcgg cttcgcggac cgtctggcgc gaggtttctt gcgcgctggc gatgatgccg
     7141 tcggcctcct gacgtgcctc gtgcaaagtc ttttcgacct cggcttcgag ggccgcctgg
     7201 tctttcttca tctgttcacc gagcgtcaca ccttcctcga tggtcttacg gcgttcatcc
     7261 atcatgcgga tgatcggctt gaaggcgaag cgttccaaca cccagaacgc cagcaaaaag
     7321 ctgaccagct ggattatcag cgccttgcca tcaaggccga gcgcgccgat acccccggac
     7381 gaactttccg caaattgtgt tactgaactt atcatctaca acaatattcc actgttatga
     7441 ggatttttcc gcgatagtgc gatttgttcg aaagccgagc accgcaagcg agccgtactg
     7501 acaggtacgg tgagtgcgga gcgcagggtt tcgggcaaag cgtgcatcgt ggtaaatcct
     7561 ttatttgaca aagaaggttg cggcgaaggc taagagtccg tagacctcga ccagaccgac
     7621 gaagaccaga gcctggccta agaagcggga agcttccggg ttgcggccga cggcggccag
     7681 atagttggca ccgatcagac cgataccgat ggtcgggccg ataaagccgg cgccgatcat
     7741 gatgccgtgg gccaatgtct gggtagcctc gggctgggtc acggtcgcgg caagatgtgc
     7801 agcaagtggt acg
//
LOCUS       DASSFD010000056         4617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1199847, whole genome shotgun sequence.
ACCESSION   DASSFD010000056 DASSFD010000000
VERSION     DASSFD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199847"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFH39_01300"
     CDS             complement(<1..440)
                     /locus_tag="VFH39_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6924450.1"
                     /translation="MIKAVLFDAGGVLHVKNTAVGDDFQQEFGLSHEQVMEIFRHYVP
                     LIGTGRMTEQQCLDDMAEKWHLRRATESERLFSRGFETTLEKTPGIYDLIDGLKKQGI
                     TVAVFSNVTPQFAEVQAQAGHYDPFDYRILSYEAGMWKPDPAFY"
     gene            552..2366
                     /locus_tag="VFH39_01305"
     CDS             552..2366
                     /locus_tag="VFH39_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET6924451.1"
                     /translation="MRIPDSLVEKLLKGTGKFTDEQLAALREQETHDKKPLQDIVLKN
                     GVLSEKELTKLYADEIEVAFIELNPREIKKEVLKALPERIARQYKAVVFEAEKGQPMS
                     IAMEDPDDIQALNFLHKQLGTDLKIYITTASIIQGALDQYRGNISSELTKVIAGDEIE
                     TVEDEDVSEDDVAEDSPIAQTVNLIIEYGVKAGASDIHIEPRDGYVAVRYRIDGLLRE
                     ANKLPRKVLGALVSRIKILSNLKIDEHRAPQDGRFKVQISGQMYALRVSTLPIVDGEK
                     VVMRILNESSKSATLEELGFWGDALRLLQHAIIQPHGMVLVTGPTGSGKSTTLFSVLS
                     MLNTPNVNISTVEDPVEYRIVGANQTQVNPVAGMTFGSGLRALLRQDPNIIMVGEIRD
                     GETADLAVQAALTGHLVFSTLHTNNAATCLPRLLNMGVEPFLIASTVRAVIAQRLVRR
                     LCVDCRETYTPDAELVKRIEKVFGVGSHFTYKTLHGFETAALSDGIGKLDSAGKGGHD
                     SDTLSSNDNAIGRLFKAHDGGCDSCNHTGYKGRIGIYEVLDNSENVQKMIVSNSTSEQ
                     IEAQAIKEGMLTMQLDGLVKSLRGETTIEEVMRVTADE"
     gene            2376..3665
                     /locus_tag="VFH39_01310"
     CDS             2376..3665
                     /locus_tag="VFH39_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6924452.1"
                     /translation="MTKFVYKALKDGKAIEGEMEAPNKEALIGALTKQGLHPLVIKQG
                     KTKGGGGFFSMSAGSKKVKLQDLVIFTRQLSTMISAGVPLARSLAALANDSESPYLRE
                     VLTGITKEVEGGAPLGDAFAKYPNVFSDVYVNMVRAGENGGILDEILKRLAAQVEQDA
                     SIRKKIKSAMMYPIVILSVTVLAFFGIMLFIVPKIGSIMTSLAGPNAKLPIYTRVLLH
                     FSDMCVHATIVHAVPVINKIPVISKLPNLIFILIVMGIAGFYLMRYIKTPEGKYKFHA
                     VLLRIPIIKVIILKIAIARFSRTFASLMSSGVSVLDALAVTGGAIGNKVIEAELKAAA
                     AEVKNGKQLSEPLSTSKHFPPIVSQMLAVGEETGQMDTVLVKVADFYEEEVSTMIDGL
                     AAIIEPIMIIFLGAGVGLIAASVMGPIANLSKQIGTS"
     gene            complement(3668..3961)
                     /locus_tag="VFH39_01315"
     CDS             complement(3668..3961)
                     /locus_tag="VFH39_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924453.1"
                     /translation="MTAPEQVVYDNDNAEAGRSGSRSIKKMGVIGVTLLLTSCHAGCH
                     ANAHHAGGSVGMGVEVAPGSRSHEPALTPAAAQVTPVDMRVGHQTVDLAEATR"
     gene            4148..>4617
                     /locus_tag="VFH39_01320"
     CDS             4148..>4617
                     /locus_tag="VFH39_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET6924454.1"
                     /translation="MISLKKQNKGFTIVELLIVIVVIGILAGLVITTYNGIQQKARNT
                     ERTTDLKTFQSQLEAYYAQNGRYPMIATDGTGLGGDSAQNVAFIQTNMKGMDKETLRD
                     PKGASGDYSLNTGTTLGGNKYTYNPAPTGCDYSGTNDCTSYTLTAVPEGGGSNIV"
BASE COUNT         1096 a         1295 c         1322 g          904 t
ORIGIN      
        1 tcatagaaag ctgggtccgg tttccacatc cccgcctcgt acgacaggat gcggtaatcg
       61 aaggggtcat agtgcccggc ctgggcctgt acttcggcaa actggggcgt gacattactg
      121 aaaacggcga cggttatgcc ctgcttcttc agcccatcaa tcaagtcata tataccgggt
      181 gtcttttcga gggtggtttc gaagccgcgc gagaagagcc gctcggactc ggtggcacga
      241 cggaggtgcc acttttcggc catatcatcc aagcactgct gctcggtcat gcggcccgtg
      301 ccgataagcg gcacgtagtg tcggaatatc tccatgacct gctcgtggct cagaccgaac
      361 tcctgctgaa agtcatcgcc tacggcagtg tttttgacgt gcaacacccc gccggcgtcg
      421 aacagaacag cttttatcat gcttggcatt atagcaacgg tgtagcgcag cccaatgaag
      481 cgcttacggt ttttgggtag cggtgctaaa atgtttccag gatgggtggg ctttaaaaca
      541 ccgaaacaaa catgcgtata cctgactcat tagtcgagaa actgctcaag ggcaccggca
      601 agttcaccga tgaacagctg gcggctttgc gtgagcagga aacgcacgac aagaagccgc
      661 tgcaggacat cgtgctcaaa aacggggttt tgagtgagaa ggaactgacc aagctgtacg
      721 ccgacgagat cgaggtggcg ttcatagagc tcaacccgcg cgagataaag aaagaagtgc
      781 tgaaagcgct gccggaacgg attgcccgcc agtataaggc agtggtgttc gaggccgaaa
      841 agggtcagcc tatgagcatt gccatggagg acccggacga tatccaggcg ctgaacttcc
      901 tgcacaagca actcggcacc gacctgaaga tatacatcac gaccgccagc atcatccaag
      961 gcgcgctcga ccagtaccgc ggcaacatca gcagcgagct gacaaaagtg attgccggcg
     1021 acgagatcga gacggtcgaa gacgaagacg tcagtgaaga cgacgtagcc gaagactcgc
     1081 caattgccca gacggtgaat ctgattatcg aatacggcgt gaaggccggc gccagcgaca
     1141 tacatataga accgcgcgac ggctacgtgg cggtgcgcta ccgcatagac gggctgctgc
     1201 gcgaagccaa caaactgccg cgtaaggtgc tcggtgcgct ggtgagccgc ataaagatcc
     1261 tgagcaatct gaagatcgat gagcatcgtg caccgcagga cggccggttc aaagtgcaga
     1321 tatccggcca gatgtacgcg ctgcgtgttt ccaccctgcc gatcgtggac ggtgaaaagg
     1381 tcgtcatgcg aatacttaac gagtcaagta aatcggcaac gctggaagaa cttgggttct
     1441 ggggtgacgc cctgcgcctg ctacagcacg ccatcatcca gccgcacggt atggtgctgg
     1501 tgaccggccc aaccggctcc ggcaagtcca ctaccctgtt cagtgtcctg agtatgctga
     1561 atacacctaa tgtgaacatc agtaccgtcg aagacccggt ggagtaccgg atcgtcgggg
     1621 cgaatcagac gcaagtgaac ccggtggccg gtatgacgtt cggcagcggt ttgcgcgccc
     1681 tgctgcggca agaccccaac atcatcatgg tgggcgagat ccgtgacggc gagacggccg
     1741 acctggccgt acaggcggcc ttgacggggc acctagtgtt cagcacgctg cacaccaaca
     1801 atgctgccac ctgtctgccc cgcttgctca acatgggtgt ggagccgttc ctgatcgcca
     1861 gcaccgtgcg ggccgtcatt gcccagcgcc tggtgcgtcg actgtgcgtc gactgccgcg
     1921 aaacgtacac gccggacgcc gagctcgtga agcggatcga gaaagtcttc ggtgtgggga
     1981 gtcacttcac ctacaaaaca ctgcacggct tcgaaaccgc tgctctgagc gacggcatcg
     2041 gcaagctcga ctcggccggc aagggcggcc acgacagcga caccctcagc tccaatgaca
     2101 acgccatcgg ccggctgttc aaagcccacg acggcggctg cgacagctgt aaccacacag
     2161 ggtacaaagg ccggatcggt atttatgaag tgctcgataa cagcgagaac gtccagaaga
     2221 tgatcgtcag caactcgacc agcgagcaga tcgaggctca agccatcaaa gaaggcatgc
     2281 tgaccatgca gctggacggc ctggtcaagt cattgcgggg cgaaacgacc atagaggaag
     2341 tcatgcgcgt gactgcggat gaataggatt aagagatgac gaagttcgta tacaaagcac
     2401 tcaaggacgg caaagccata gaaggcgaga tggaggcgcc caacaaagaa gcgctgattg
     2461 gcgccctgac caagcagggg ctgcacccgc tggtcatcaa acagggtaag acaaagggcg
     2521 ggggcggttt cttcagcatg agcgccggct cgaagaaggt gaagctgcag gatttggtca
     2581 tctttaccag gcagttgtcc accatgatat ccgccggcgt gccgctggcc cgttcgctgg
     2641 ccgccctggc caacgacagc gaaagcccct acttgcgtga ggtacttact ggcataacca
     2701 aagaggtgga aggtggtgcg ccgctcggcg atgcctttgc caaatatccg aatgtcttca
     2761 gtgatgtcta tgtgaacatg gtccgcgccg gcgagaacgg cggtatcctg gatgagattt
     2821 tgaagcgcct ggcagcccaa gtcgagcagg acgccagcat ccgcaagaaa atcaagagcg
     2881 ccatgatgta ccctatcgtc atcctgtcgg tcaccgtcct ggcgttcttc ggcatcatgc
     2941 tgttcatcgt gccaaagatt ggctccatca tgacgtcgtt ggccggtccg aacgccaaac
     3001 tgcccatcta cacccgtgtg ctcctgcact tcagcgacat gtgcgtacac gcgaccatcg
     3061 tgcacgccgt gccggtcatc aataagattc cggtcatcag taagctaccc aacctgattt
     3121 ttatcttgat agtgatgggc atcgccggct tttaccttat gcgttatata aagacgccgg
     3181 agggaaagta taagttccac gccgtactgc tgcgcatacc gatcatcaag gtcatcatcc
     3241 ttaagatcgc cattgccagg ttctcgcgca cgtttgcgtc gctgatgtca tctggcgtca
     3301 gtgtgctcga tgccttagcc gtcaccggcg gagctatcgg taacaaagta atcgaggctg
     3361 agctgaaagc agccgctgcc gaagtgaaga acggcaagca gctgagcgag ccgctctcca
     3421 ccagcaagca cttccccccc atcgtgtcac agatgctggc ggtcggcgaa gaaaccggcc
     3481 agatggacac cgtcctcgtg aaggtcgctg acttctacga agaagaggtc agcaccatga
     3541 tcgacggttt ggcggccatc atcgagccga tcatgatcat attcttaggc gccggcgtgg
     3601 gtctgatcgc tgccagcgtc atgggcccga tagccaacct gtcgaagcag atcggcacca
     3661 gctaggctta gcgcgtggct tcggcaaggt cgaccgtttg gtggcctacg cgcatatcca
     3721 ccggggtgac ctgtgcggcg gcaggtgtta acgccggttc gtgactcctg ctgcccgggg
     3781 ctacttcgac ccccatacca acacttccgc ccgcgtggtg agcattggcg tggcaaccgg
     3841 catgacaact ggtgagcagc aaggttacgc cgattactcc catcttcttg attgagcggc
     3901 ttccggagcg tccggcctcg gcattatcgt tgtcatatac tacctgttct ggtgctgtca
     3961 tggcggtcgt ccctccttag ttaacgtcct acaatgtagc gcagggagcc ggaacggtga
     4021 gatgaaattt attacaacaa gcttgacggc ggtgcgctta tggttataat cagtggcaga
     4081 tgctttatat aagcgttaac accttataaa aagatcaata caattaaatc aaggagggtg
     4141 ggtccttatg atctcactta aaaagcaaaa caaaggtttc accattgtgg aacttttgat
     4201 cgtgattgtc gtgatcggta tcttggccgg cctcgtcatc actacgtaca acggtattca
     4261 acagaaggcg cgcaacaccg agcgcaccac cgacctcaag acgttccaga gccagcttga
     4321 ggcttactac gcccagaacg gccgctaccc gatgatcgca accgacggca caggtttagg
     4381 tggcgacagc gctcagaacg tagcattcat tcagaccaac atgaagggca tggacaaaga
     4441 gacccttcgt gacccgaagg gcgcgtctgg cgactactca cttaacactg gcacgactct
     4501 cgggggcaac aaatacacct acaatcctgc cccgacaggc tgtgactaca gcggtaccaa
     4561 cgactgtacg agctatactc taactgctgt acccgaaggt ggtggcagca acattgt
//
LOCUS       DASSFD010000057         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1796390, whole genome shotgun sequence.
ACCESSION   DASSFD010000057 DASSFD010000000
VERSION     DASSFD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796390"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2427
                     /gene="gyrA"
                     /locus_tag="VFH39_01325"
     CDS             <1..2427
                     /gene="gyrA"
                     /locus_tag="VFH39_01325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6924455.1"
                     /translation="LRYSMSVIVSRALPDVRDGLKPVHRRILYTMNRDGLRSGAKHRK
                     SANVVGAVMGDYHPHGDAAIYDAMVRMAQPWSMRYLLVNGQGNFGSMDGDPPAAMRYT
                     EAKMHRLAEELLVDIDKETVDFRDNYDGRLQEPSVLPAKLPNLLLNGQLGIAVGMATN
                     IPPHNLSELVDATVEQIDNPDATLDDLLEHVKGPDFPTGGLVYGKESLRTAYATGKGG
                     VVTRGVAEVVEGQKGRHQIIITEIPYALNKESLIIKIADLVRDKKIAGVADIRDESAR
                     GNVRIVIDLKKDAFPKKLLNQLFKLTPLQTSFHFNMLALVDGIQPRILGLQDIIQEHI
                     KHRRTVVRRRTEFELRKARERAHILEGLKIALDHIDEVIATIRASETTEEAQENLVKK
                     FKLTEIQARANLAMQLRTLAGLERKKIEDELADLLKLIEKLESILADEKKILKIVKDE
                     LLELKKQYGDERRTKVVAGELGKMSDEDLIAEEQVVVTLTSANYIKRSPIAEYKRQGR
                     GGKGRRGMATREEDVIEHVVNASTHDFLLFFTNKGRVFRIKTYEVPAASLNAKGVALV
                     NLLQLQPEETVSSVINVSKGDQAANLIMCTVRGTVKKTPFEQYQNVRSSGLIAINLDE
                     GDELKWIRPTTGDNEVVISTSNGQAIRFHEKDARPMGRVSRGVRGIRLRPGDRVIGMD
                     IVEQGSDIFVISKYGYGKRTKIAQFTPHARGGVGIRSAVVNTKTGELIGVKTLAAGTE
                     QEVIIISRNGQTIRLGLKDIPQLGRATQGVRIMRLNDGDEVVSLALVDKAEDIDEAGE
                     DDVVLEASAE"
BASE COUNT          643 a          744 c          781 g          445 t
ORIGIN      
        1 ttgcgctact ccatgagcgt cattgtcagc cgggcgctgc cggacgtgcg cgacgggctg
       61 aagccggtgc atcggcgtat tctctacacc atgaaccgcg acggcctgcg cagcggggcc
      121 aagcaccgca agagtgccaa cgtcgtcggt gccgtgatgg gtgattacca cccgcacggc
      181 gacgccgcca tctatgacgc catggtccgc atggcccagc cgtggagtat gcgctacctg
      241 ctggtgaacg ggcagggtaa cttcggcagt atggacggcg acccgccggc cgccatgcgt
      301 tacaccgagg ccaaaatgca ccgtctagcc gaagagctcc tggtggatat agataaagag
      361 accgtggact tccgcgataa ctacgacgga cggctgcagg aaccgtccgt actcccggca
      421 aaattgccga acctgttgct aaatggacaa ctgggtatcg ccgtcggcat ggccaccaac
      481 attccgccgc acaacttgag cgaactggtg gacgccacgg tagaacagat agataacccc
      541 gatgcgacgc tggacgactt gctcgagcat gtgaaaggtc cggatttccc gacgggcggt
      601 ctggtgtacg gcaaggagtc actccgcacg gcgtacgcta ccggcaaggg cggggtggtt
      661 acgcgcggcg tagccgaggt ggtggaaggc caaaagggcc gccaccagat aattatcacc
      721 gagataccct acgcgctcaa caaggaaagc ctgattatca agatcgccga cttagtacgc
      781 gacaagaaga tagccggcgt agccgacatt cgcgacgaaa gtgcccgcgg caatgtgcgc
      841 atcgttatcg acctcaaaaa ggacgctttc ccgaagaagt tgctgaacca gctcttcaag
      901 cttacgccgc tccagaccag ctttcacttc aacatgctgg cgctggtgga tggcatacag
      961 ccgcgtatcc tggggctgca ggacattatc caggagcaca ttaaacaccg ccggacggta
     1021 gtgcgccgcc gtaccgagtt tgagttgcgc aaagcccgcg agcgtgccca cattctggaa
     1081 gggctgaaga ttgcactcga ccacatcgat gaagtcattg ccaccatccg cgccagtgaa
     1141 accaccgagg aggcgcagga aaaccttgtt aagaaattta agctcaccga gattcaggcg
     1201 cgcgccaacc tggcgatgca gctgcgtacg cttgccgggc tggaacgcaa gaaaatagaa
     1261 gacgaactgg ccgacttgct caaactcatc gagaagctcg aaagcatcct ggccgacgaa
     1321 aagaagatac ttaagattgt taaggacgag ctgctggaac tgaaaaaaca gtacggcgac
     1381 gaacgccgca ctaaagtcgt agccggcgag ctcggtaaga tgagcgacga ggacctgata
     1441 gccgaagagc aggtcgtagt caccctcacc agcgcgaact atatcaagcg cagccccata
     1501 gccgagtaca agcgccaggg ccggggcggt aagggacggc gcggcatggc tacgcgcgaa
     1561 gaagacgtca ttgagcatgt cgttaatgcc agcacgcacg acttcctgct gttctttacc
     1621 aacaagggcc gggtgttccg tataaagacc tacgaagtgc cggcagcctc cttgaatgcc
     1681 aagggcgtgg cgctggtgaa cctgctgcaa ctacagcccg aggaaacggt cagcagtgtc
     1741 atcaacgtca gtaagggtga ccaggctgcg aacctcatca tgtgcaccgt acgcggcact
     1801 gtcaaaaaga ctccgttcga acagtaccag aatgtccgca gcagcggtct catagccata
     1861 aacctggatg agggtgatga gctgaagtgg atccgcccca ccacgggcga taacgaagtc
     1921 gtcatcagca ccagtaatgg acaggctatc cgcttccacg aaaaggacgc ccgcccgatg
     1981 ggccgcgtca gccgcggtgt acgcggtatc cggctgcgac ccggtgaccg cgtgatcggc
     2041 atggacatag tagagcaggg cagcgacatc ttcgtaatca gcaagtacgg ttatggcaag
     2101 cgcaccaaga ttgcccagtt tactccgcac gcccgcggcg gcgtcggtat ccgcagtgcg
     2161 gtcgtgaaca ccaagaccgg tgagcttata ggtgtcaaga cgctggcggc cggcaccgaa
     2221 caagaggtaa tcatcatctc caggaacggt cagaccatcc ggctgggtct caaagacatc
     2281 ccgcagctcg gccgcgccac tcaaggtgtg cgtatcatgc gcctgaacga cggcgatgaa
     2341 gtcgtgtctc ttgctctggt agataaagcc gaagacatcg acgaagccgg ggaagacgac
     2401 gtcgttctgg aagcatccgc cgaataaacc gcggcccagt gggtttttta actgccccgg
     2461 atgctccggg cggtatatag ttgcccttta ataaaaggga gacggccatg gacaaggagc
     2521 agcttagaac gaggagtatc gaagaaagcc gatcggccat taaggcggcc aggatgatcg
     2581 gcagctggat aaccaggcgg tccgctatgc tta
//
LOCUS       DASSFD010000058         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_684515, whole genome shotgun sequence.
ACCESSION   DASSFD010000058 DASSFD010000000
VERSION     DASSFD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684515"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFH39_01330"
     CDS             <1..1090
                     /locus_tag="VFH39_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924456.1"
                     /translation="RVVMKLVHHLLRYVLISTFLVGSATTGITAAQAPPATADAPARP
                     HQLKCPKWYNGDHVLSCWLTAWHPTGQKASIPNKIGNWQDCGFFTPSMYPGGTMHCRV
                     SKSTSTTRSNTVTVSAGIDINIKDIIDISASIKKVTHYSITHKTTVSAWADKPVPGPY
                     NGSIQWAAVWGHRYRVQLKSTKEAYYVTDSTSGAHTLFGRVKPHYSYIDTGEVLNAAA
                     YRVVYDKWKATADASYQYYRIGGWQTCARFSRKAHSRNITCRVDTKVSTEPSPMSAVR
                     ISLKEMSRLVGYDVRHTTTVRIHHAVNVDAHKSGRVEWAPGFGSRKVIHENLYQCIRN
                     RCTLKHNQPQKTVTVEKYSGNEYRVVNN"
     gene            1139..1621
                     /locus_tag="VFH39_01335"
     CDS             1139..1621
                     /locus_tag="VFH39_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924457.1"
                     /translation="MSTANHGFTPDQRATSEFAEQRTDYGRVLRAAAALGTMALLSAC
                     SGNSSPQKDGFNPDCSKNPHAANVIFAQHTGVSQTESLDGMHIPRDATLQLSLCEPGE
                     QANVTFTEGGSQSEYKISTEPGTDLPAGALSVPDNGPLTSFTITPTSDTIYYQLLVSK
                     "
     gene            1732..2265
                     /gene="msrA"
                     /locus_tag="VFH39_01340"
     CDS             1732..2265
                     /gene="msrA"
                     /locus_tag="VFH39_01340"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6924458.1"
                     /translation="METATLGGGCYWCLDAFYRRIEGVEKVVSGYSGGHVDNPTMARV
                     YEGDTGHAEVVQVTFDPSIISYREILEIFFAMHDPTTLNRQGNDVGDEYRSVIFYHSG
                     GQKVVAEDMIRNFAPTLYKDPVVTQLEPLTMFWPADEANQDYFAKNPGAGYCMVVIDP
                     KIRKLRQKFAARLKQPG"
     gene            complement(2249..2566)
                     /locus_tag="VFH39_01345"
     CDS             complement(2249..2566)
                     /locus_tag="VFH39_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET6924459.1"
                     /translation="MQAVWNGQVIADAAKEDLIYIEGNWYFPPSSVKQEFLRKSDTPY
                     TCPWKGVCQYFDVVKGAQASKDAAFSYPEPLPSAIERVHKDFSGYVAFWRDVKVQEPS
                     PVA"
     gene            complement(2586..>2867)
                     /locus_tag="VFH39_01350"
     CDS             complement(2586..>2867)
                     /locus_tag="VFH39_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924460.1"
                     /translation="GYEYLSLRGEPEHRGMIRGFVSPERTRLYETQLRAMRDFVDPTA
                     GLCLAIEMLKSYSHSRLRANDVILLPLSSQEYRLNFHEPSREWLTGAVG"
BASE COUNT          646 a          884 c          760 g          577 t
ORIGIN      
        1 gagggttgtc atgaaactgg tgcatcatct gttacggtac gtcttgataa gcacattttt
       61 ggtcgggagc gccacgacgg gcatcaccgc cgcccaggca ccgcccgcga ccgcggacgc
      121 gcccgctcgt ccacaccagc tcaagtgccc taagtggtac aacggcgatc acgtactctc
      181 ctgctggctg actgcctggc acccgaccgg ccaaaaggcc agcattccca ataaaatcgg
      241 caactggcag gattgcggtt tctttacccc tagcatgtac ccgggcggta ccatgcattg
      301 ccgcgtcagc aaatcaactt cgacgacgcg ttccaacacg gtcacggtga gcgccggaat
      361 cgacatcaac atcaaggaca taatcgacat cagtgcttct atcaaaaagg ttacccatta
      421 ctccataacg cacaagacca ccgtctcggc gtgggcggac aaaccggtac cgggccccta
      481 caacggctcg atacaatggg ctgcggtgtg gggccaccgc taccgcgtcc agctgaagtc
      541 gacgaaagaa gcctactacg tcaccgattc gaccagtggc gctcacacgc tcttcggccg
      601 cgtcaagccg cattactcat acatcgatac cggcgaagtg ctgaacgccg ccgcctaccg
      661 ggtcgtctac gacaagtgga aggctaccgc cgacgcctcc tatcagtact accggatcgg
      721 cggctggcag acctgcgccc gcttctcgcg caaagcacac tcacggaata tcacgtgccg
      781 ggtggacacc aaggtaagca ccgagccctc tccgatgtct gcggtgcgga tcagcctgaa
      841 agaaatgagc cggctggtgg gctatgatgt acgccatacg acgacggtac gaatacacca
      901 cgccgtcaac gtagacgccc ataaatccgg ccgcgtcgag tgggcgcctg gcttcggatc
      961 gcgcaaggtt atccatgaga acctatatca gtgcatccgc aaccgctgca ccctcaaaca
     1021 caatcagccg caaaaaaccg taacggtgga gaaatacagt ggcaacgaat atcgcgtggt
     1081 gaacaattag ctcgcccaca atttgcattc atatgttaaa agttatataa ttacccttat
     1141 gtctactgct aaccacggtt ttacccctga ccagcgcgca acatctgagt ttgctgagca
     1201 acgcaccgat tacggacgcg tgttgcgggc ggcagctgcc ctaggcacta tggccttgct
     1261 ttctgcctgc agcggtaaca gcagcccgca gaaagacgga tttaacccgg attgttccaa
     1321 gaaccctcac gcggcgaatg tgatttttgc gcagcacacc ggcgtcagcc aaacggaatc
     1381 gctggacggt atgcatatcc cgcgtgatgc gacactccaa ctttccctct gtgagccggg
     1441 tgaacaggcc aatgtgacgt tcactgaggg tggctctcag agcgagtata agatcagtac
     1501 cgaacccggc acggatcttc ccgccggcgc cctgagcgtg cctgacaacg ggccacttac
     1561 cagcttcacc attacgccca ccagcgacac aatatattac cagctcctcg tatccaagta
     1621 gcattggtac gtgaatagcc ccagtcgata atatctggca tacgcgcgct tagcggcctc
     1681 tcgtgccctc gctcatagat tcctgattac tgctcagcta caatgagtcg cgtggagaca
     1741 gcgacactcg gcggcggctg ctactggtgc ctcgacgcgt tctaccggcg catcgaaggc
     1801 gttgagaagg tggtgtccgg ctacagcgga ggccacgtcg acaacccgac gatggcgcgc
     1861 gtatacgagg gcgataccgg gcacgccgaa gtcgtccagg tcacgttcga cccgagcatt
     1921 ataagctacc gcgaaatcct ggaaattttc tttgccatgc acgacccgac cactctgaac
     1981 cgccagggca acgacgtcgg cgatgagtac cgcagcgtca ttttttatca ctcaggcgga
     2041 cagaaggtcg tcgcggaaga tatgattcgg aatttcgcgc cgacgttgta caaggacccg
     2101 gtcgttacgc agctggagcc gctaacgatg ttctggccgg ccgacgaagc caatcaggat
     2161 tacttcgcca agaacccggg cgccggctat tgcatggtcg tcattgaccc gaaaatccgc
     2221 aaactgcgcc agaagttcgc agctcggctc aagcaaccgg gctaggctct tgcaccttca
     2281 cgtcgcgcca gaaggcgacg tagccgctga agtctttgtg gacgcgctcg atggcactcg
     2341 gcaacggctc gggataactg aaagcggcgt ctttgctggc ctgggcgccc ttcactacgt
     2401 cgaagtactg gcacacgccc ttccacggac aggtgtacgg cgtgtcgctt ttgcggagga
     2461 actcctgctt gacgctgctc ggcgggaagt accagttccc ttctatgtaa atcagatctt
     2521 ccttggcggc gtcggctatc acctgcccgt tccagactgc ttgcatacta ttgctcctat
     2581 tcgttttaac cgacggcacc cgtcaaccac tctctgcttg gctcatgaaa gtttaaccgg
     2641 tactcctgac tggaaagcgg cagcagaatg acatcgttcg cccgcaacct gctatggcta
     2701 tagctcttca gcatctcgat agccagacac agaccggctg ttgggtcgac aaaatctcgc
     2761 attgcgcgca gttgtgtttc gtacaggcgg gtccgctcgg gggagacaaa tccgcgtatc
     2821 attcctctgt gttccggctc gccccgcaaa ctgagatatt cataacc
//
LOCUS       DASSFD010000059         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_772282, whole genome shotgun sequence.
ACCESSION   DASSFD010000059 DASSFD010000000
VERSION     DASSFD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772282"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VFH39_01355"
     CDS             <1..1995
                     /locus_tag="VFH39_01355"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET6924461.1"
                     /translation="RIISFAVNGVEPDLCIVLDAPVPVLKERAHQRGQGERFDNLDEA
                     FLERVRAGYLWEAKQRGLPVVFATEDFATVADKIWKQVTSVIAVREPSQALAASREPV
                     SIQQVIADKQATLSADGSVKAEPAASNQADEIAYVSKNDKGVYEMTEAGREYLETAVT
                     DVAGNVYGFTDKLSPVTIAAAMARLSRRGDDMRVTILDEFAGKLGKDEKLLQRVITAY
                     GDDSVQQLAGMHVVVENASNLLTKKLEWGRLAAYLEQSTRYIYYDQKDAEGKYKYFVP
                     PELSKTLKQKYVKTLDTIFDTYSDMVHKLTRYVQDNSDVPEAERDVAWKGATRAQACD
                     ACRPVLPVATKSTVGIFGSGQAIESLIMHLMSDELPEARRTGQLMLGQVRQITPTFFE
                     RADKPDRGGAAIAYRANTSKAVAQLADKYLTQQHAEGDAEPVTLNSYWPKSELSLIPD
                     MLYEHSELSLNEIRQQVENWPYERKTDVFTTYMGERLNRRHRPGRALENAHYSFDLLC
                     DYGIFRDLQRHRMVEDLAWQQLTPRYGYDVPELIEKAGLAEQYEECFQLSLQLHSALQ
                     KAGYTLEAQYATLLGHKMRWKVSYNAREAFHLHELRTSPQGHSGYRKLVQQMHEKVAE
                     VHPLLAEAMIFVNKGEDEKLTRLAAERYTQYKLAQLDKKA"
BASE COUNT          529 a          594 c          614 g          419 t
ORIGIN      
        1 cgcatcatca gctttgcggt caacggcgtc gagccggacc tttgtatcgt cttagacgcg
       61 ccggtgccgg tactcaaaga acgagcccac cagcgggggc agggcgagcg cttcgacaac
      121 ctagatgaag cattcttaga acgggtacgg gctggttacc tctgggaagc gaagcagcgc
      181 gggctgcccg tcgtctttgc gacagaagat ttcgccaccg tcgctgacaa gatctggaaa
      241 caagtaacca gcgtcatagc ggtgcgcgag ccgagtcagg cgttagccgc cagtcgtgaa
      301 ccggtgtcta tccagcaggt gattgctgat aagcaggcga ccctatcggc ggacggtagc
      361 gtaaaggccg aaccggctgc cagcaatcaa gcggacgaaa tcgcctatgt atctaaaaac
      421 gataagggcg tatatgagat gactgaagcc gggcgcgagt atctggagac tgccgtcacg
      481 gacgtcgctg ggaacgtcta cggctttacg gacaagctca gtccggtcac cattgccgcc
      541 gccatggcca ggcttagccg gcggggtgat gatatgcgcg ttactatcct cgatgaattt
      601 gccggtaagc tgggcaagga cgaaaagttg ctgcagcggg ttataacggc ttatggagat
      661 gattccgtac agcagttggc cgggatgcat gtggtggtcg aaaatgccag caacctgctc
      721 accaagaagc tggaatgggg gcgactggca gcgtacctgg aacagtcgac gcgctatatt
      781 tattacgacc agaaggacgc tgagggcaaa tacaaatatt tcgtgccgcc cgagcttagt
      841 aagaccctta aacagaagta tgttaagacg ctcgacacga tctttgatac gtattccgac
      901 atggtgcaca agctcaccag atacgtccag gacaactccg acgtgccgga agcagaacgt
      961 gacgtcgcct ggaagggtgc tacccgggca caggcctgtg acgcctgccg acccgtgctg
     1021 cctgtggcga cgaaatctac cgtcggcatc ttcggttccg gccaagcgat cgaaagtctt
     1081 atcatgcacc tgatgagcga tgagttgccg gaggcacgcc gcaccggtca gctgatgctc
     1141 ggtcaggtgc gccagataac gcccacgttc ttcgaacggg ccgataagcc tgatcgcggc
     1201 ggcgcagcta tcgcttatcg ggcaaatact tccaaggccg tcgcccagct ggccgataaa
     1261 taccttaccc agcagcacgc cgaaggtgat gctgagccgg tcaccctgaa ttcgtactgg
     1321 ccgaagagcg aactgagcct gattcccgat atgctgtacg agcacagcga gctgtcgctc
     1381 aatgaaatcc gtcagcaggt tgaaaactgg ccttatgaac gcaagaccga tgtctttact
     1441 acctacatgg gagaacggct caatcgccgg catcgccccg gccgggcact cgaaaacgca
     1501 cattacagct ttgacctgct atgtgactac ggtattttcc gcgatctgca gcgtcaccgc
     1561 atggtcgaag acctggcgtg gcagcaattg acccctcgct acggttatga cgttccggag
     1621 cttatcgaaa aggccggcct tgccgagcag tacgaggagt gtttccagct cagcctgcag
     1681 ttgcacagcg ccctgcagaa ggcaggctac actctagagg cgcagtatgc gaccttgctc
     1741 ggccacaaga tgcgctggaa agtcagttac aatgcccgcg aggcgttcca cctgcacgag
     1801 ctgcgtacca gtccacaagg acattcgggc tatcgcaaac tagtccagca gatgcatgaa
     1861 aaagtcgccg aagtccatcc tttgttagct gaagccatga tcttcgtcaa caagggtgaa
     1921 gacgagaaac tgacccgctt ggcggccgaa cggtataccc agtacaaatt ggcgcagctg
     1981 gacaaaaaag cttgacacgt aaactttaac aagcgtataa caggtacaaa tggcaaacgt
     2041 agtgaagcgc tttatgatta ttgtcctcgg cctcaacggg ctgtgcttca ccatggttga
     2101 agccacacga caataagtcg tccgcacagt ccagaaaacc aagtaggctt caaccg
//
LOCUS       DASSFD010000060         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_156235, whole genome shotgun sequence.
ACCESSION   DASSFD010000060 DASSFD010000000
VERSION     DASSFD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156235"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFH39_01360"
     CDS             complement(<1..479)
                     /locus_tag="VFH39_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924462.1"
                     /translation="MKLDFHHPRYVRAQAGRRLSKNGYGLLWALAVLLGGAGIALSLL
                     HYRWGVSLVGVAIIPAMVALWWRRDLSVVPVAGTGLNDRLDVAVLQRLHAGPEPYTPS
                     TLWQAIQPHWQARFFTNHLLLPPQFMTDQLATLTNDLNAIFSTAAELADRTGCQAVE"
     gene            complement(479..838)
                     /locus_tag="VFH39_01365"
     CDS             complement(479..838)
                     /locus_tag="VFH39_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924463.1"
                     /translation="MLAIKFLTWWYTAGWRDAGRRVNRQLSSVSDAFSVGILLRTLFA
                     PWRRIISYPGAGIDAHIHAALDNAVSRFIGFLVRMMALIAASVMLLVSMIGGAVVLLL
                     WPLLPLLALGLIAWGLA"
     gene            complement(931..>2011)
                     /locus_tag="VFH39_01370"
     CDS             complement(931..>2011)
                     /locus_tag="VFH39_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HET6924464.1"
                     /translation="ICGSDLHMYGGRTGVEEGKVLGHEIMGIIEEVGDGVNQLKVGDR
                     VVLPFNIACGNCYNCVRGWTNACLTMNPESAGAAYGYAAMGPYQGGQAEMVRVPFADF
                     NALKLPGNENDELEHDFLMLADIFPTAWHACELADVTIGKSVVIYGAGPVGLLSILSA
                     RLRGAAEIYCIDAVDSRLAKAREMGAIPIDFRKGKPSEQIIALRKKSLLADAMRPGEE
                     KRLQGVDCGIDAIGYQAHDFKNPADEEANEVLEDLAAVVVPTGHVGLIGVYMPDDPGA
                     PNDDLKQGIMKMPIGLFWSKGIAIGMGQCPVKKYNEFLRDMIIAGKARPGSIVSHDVP
                     LTDAAEYYQRFNEREEGMTKVVLHP"
BASE COUNT          393 a          632 c          592 g          394 t
ORIGIN      
        1 atttctacgg cctggcagcc ggtccggtcg gccagctcgg cggcggtcga aaaaatggcg
       61 ttcaggtcat tcgtcagcgt ggctaactga tcggtcatga actgcggcgg tagcagcaag
      121 tgattggtga agaagcgcgc ctgccaatga ggctgaatag cctgccataa ggtgctcggc
      181 gtgtacggtt ctgggccggc gtgcagccgt tgtaacacgg cgacgtcgag ccggtcgtta
      241 agcccggtgc cggcgaccgg cacaaccgac agatcgcgcc gccaccacaa ggctaccatc
      301 gccggaatga ttgccactcc caccaatgac actccccacc gatagtgcag caggctcagg
      361 gcgatacctg cccccccaag caataccgcc aaggcccata gcagtccgta gccgtttttg
      421 ctgagtcgcc gtccggcctg cgcccgcaca tatcttggat ggtggaagtc gagtttcatc
      481 aggccaatcc ccacgcgata agtccgaggg cgagtagcgg cagcagcggc cacaacagca
      541 acacgaccgc accgccgatc atggacacga gcagcatgac gctggcggcg atgagcgcca
      601 tcatccgcac cagaaatccg atgaatcggc tgacggcgtt atcgagcgcc gcatggatat
      661 gggcgtcgat gccggccccc ggatagctga tgatgcgccg ccaaggggcg aacagggtgc
      721 gcagcaggat gccgaccgaa aaggcgtcgc tgacgcttga aagctgtcgg ttgacccggc
      781 ggccggcatc gcgccaccca gcggtgtacc accaggttaa gaattttatg gcaagcataa
      841 ctggtattgt accattcacc gcttggctgg gacgaaaaca gcggccctgc tataaagccg
      901 ctgttcatgg catcatacgg ataggtgccg ctacgggtgc agtacgacct tcgtcatgcc
      961 ctcttcgcgc tcgttgaagc gctggtagta ctcggcggca tcggtaagcg gcacgtcatg
     1021 gctgacgatg ctgcctggac gcgctttgcc ggcgatgatc atatcgcgca ggaactcatt
     1081 gtacttcttg accgggcact gtcccatacc gatggcgata cctttgctcc agaacagacc
     1141 gatgggcatt ttcataatgc cttgcttcag gtcgtcgttc ggtgcgcccg ggtcatccgg
     1201 catatagacg ccgattaaac cgacatgccc cgtcggtacc acgactgctg ccaggtcttc
     1261 cagcacttca ttcgcttcct catccgccgg attcttgaag tcatgcgcct ggtaaccgat
     1321 ggcgtcaata ccgcagtcga ccccttgcag gcgtttctct tcaccgggcc gcatggcgtc
     1381 ggccagtagc gatttcttac gcaaagcgat tatctgctcg ctcggtttac ctttacggaa
     1441 atcaatcgga atggcaccca tctcacgggc cttggccagg cggctgtcga cggcgtcgat
     1501 gcagtaaatt tcggcggcac cgcgcaaccg ggcactgagt atgctcagca ggccgaccgg
     1561 gccggcgccg tagatgacaa cgctcttccc gatggtcacg tccgccagct cgcaggcgtg
     1621 ccaggcggtc gggaatatgt cggccagcat caggaagtcg tgctccagct cgtcattctc
     1681 gttgccgggc agctttaggg cgttgaagtc ggcgaacggt acccgtacca tttcggcctg
     1741 accgccctga tacggtccca tggcagcgta gccgtaggcg gcgccggccg attccgggtt
     1801 catggtcagg caggcattcg tccagccgcg gacgcagtta taacaattgc cgcaggcgat
     1861 gttgaacggc agcactaccc ggtcgccgac cttcagctga ttcactccgt cgccgacctc
     1921 ttcgatgatg cccatgatct cgtgcccgag caccttgcct tcctctactc cggtccggcc
     1981 accgtacatg tgcaggtccg aaccgcagat g
//
LOCUS       DASSFD010000061         6954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_554222, whole genome shotgun sequence.
ACCESSION   DASSFD010000061 DASSFD010000000
VERSION     DASSFD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554222"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..417)
                     /locus_tag="VFH39_01375"
     CDS             complement(25..417)
                     /locus_tag="VFH39_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924465.1"
                     /translation="MATVDYFERKLDDKRRLTIPTELRAKFAHGAVITRGFGKYLHLY
                     PTEVWDNLVEPRLEGDILDERVADLNIQFRMGKVDVQPDDKQGRITIEQHLLDYAGVD
                     RDIVAVSVGGKYWRLMSPEAANQAPTAR"
     gene            complement(607..999)
                     /locus_tag="VFH39_01380"
     CDS             complement(607..999)
                     /locus_tag="VFH39_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924466.1"
                     /translation="MSELSPVDESVAEVNKWWRSPLGAGLALASAALLLTACEREHNP
                     YEFDPTACHRVTHLRLGERNLTDVAERATGYGEDTGHNDQLSAMTSAIADAENKSPHF
                     KGFDPNEVQIGQTILLPPSALTGHKSDC"
     gene            complement(1067..1924)
                     /locus_tag="VFH39_01385"
     CDS             complement(1067..1924)
                     /locus_tag="VFH39_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924467.1"
                     /translation="MEFIPSAQRERSKAFDEIVTATDQLLHDPHVYNELSPDLQQRYE
                     KVIAGAFLPDVRHGVAELPRDQEGGNGFAILPLLSQDEKKGIRLAPSMFTPTADRFDK
                     IHADLAARYGFTPMQSVDAISPQTWDMQNLIERNVAAMPARVHARTTSIGEHLNDNGP
                     LITARPTMIVGINTVKSGRLKKGAVALHELWHGMDVERLTRLPSNLEDWVTYSESKAG
                     RITHLCLEAAGVVAGDQVVDREYVTAEGFWACLQFERMRRQANPAENPYATNPQLVER
                     ITRAQRKAQ"
     gene            complement(1934..2242)
                     /locus_tag="VFH39_01390"
     CDS             complement(1934..2242)
                     /locus_tag="VFH39_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924468.1"
                     /translation="MNITKQIVKVEKEVVRDLRKLEQLEVQHRTGLERAGALALTALI
                     AFSMADFGHGDDRRNLNRVTKEAVVNQSVVFSPAEKNETVRMPVKFDDGLRATATTGY
                     "
     gene            complement(2291..3292)
                     /locus_tag="VFH39_01395"
     CDS             complement(2291..3292)
                     /locus_tag="VFH39_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET6924469.1"
                     /translation="MADASIHHLNVVTIGGGTGSFTLLSRFKEYFTGLTAVVNMADDG
                     GSTGLLRDELGVLPPGDVRQCLVALSDAPELRALFNYRFSEGSLKGQSFGNLFLSAAE
                     KVGQDFETAVELASHVLRIHGKVVPVTTDRSELVLHWGAETIRGEYRIGNLDFGDRTR
                     PKLSLEPTAHLSRAAQDAILAADYVVIAPGNLYGSLAPALIVDGMAEVLDSTEATVLY
                     ACNLVTKPGQTDGFKVHDYAAEIERFVGRPVLDYVLYNTDRPDQAVLDKYIHAGELLV
                     EADPRKLSVASYEAIGLPLIAHDFVSPDRKGDAIAASRTLIRHDAATLATAIQQLHA"
     gene            complement(3327..3965)
                     /locus_tag="VFH39_01400"
     CDS             complement(3327..3965)
                     /locus_tag="VFH39_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6924470.1"
                     /translation="MKITKFVHSCLLVEMPAPVNRTVLFDPGSMSAPALDVDQLEYLD
                     DIVITHSHGDHIDMDLIKRLVAKFPQVRITATQEVVDILSQEGITASTTPPEGMALFD
                     SPHEAVEPMFPTPEQIGVHYLDMLSHPGDSHSFNETKPILALPVTAPWGATVKAVNLA
                     LQLKPRYIIPIHDWHWNDDARANMYAGMAKVFADQGITFLKPETGVPMVVEL"
     gene            complement(3989..4864)
                     /locus_tag="VFH39_01405"
     CDS             complement(3989..4864)
                     /locus_tag="VFH39_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6924471.1"
                     /translation="MATMTNSKPSALSAGYVKIGHYRINRLGFGAMRVTGEGIWGPPR
                     DPMEARRVLKRAVQLDVNFIDTADAYGPAVSEQLIHDTLHPYEGLLIATKGGYTRSGP
                     GRWQLDGSPEHLRQALDDSCRRLGVDQITLYQFHHPDPRVDFETSVQTLVDLKEEGRI
                     RHIGLSNVSLLQLKTALTMTEIVSVQNYYNVTHRRESDPIVDFCEAHGIVFIPYYPVG
                     GGMNDFSQRTLLSIAEAHQATPQQIALAWLLARSPIMLPIPGTSSVRHLEENVAATGI
                     ELTDDDLRRLQTLSD"
     gene            complement(5004..6842)
                     /locus_tag="VFH39_01410"
     CDS             complement(5004..6842)
                     /locus_tag="VFH39_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET6924472.1"
                     /translation="MRQAQALHIMLSGKSVFLTGPPGAGKTYVLNQYIRRAEAAGKTL
                     AITASTGIAATHIGGTTIHSWSGLGIRDHLNQNDRQWLGSNDRLRKRYIATDVLIIDE
                     VSMLHGARLDMVNEACKILRENDAPFGGLQVILVGDLFQLPPVNRLSDLADFAHTSAA
                     WEELDPSICYLSEQHRQAGDELLDLLTAMRGGELDTVHLEALQSRLGVKPPADEPVTR
                     LYTHNIDVDGINQRHLQALSAEGYTFAMQTKGAAAKVEQLARSVLAPEELTLKSGAEV
                     MFVANDFAAGFVNGSRGQVIGFKDGMPLVKLTHGRIIKVEPHSWTLTEDDRKRAEVVQ
                     LPLRLAWAITIHKSQGMSLDAAEIDLSKSFTPGMGYVALSRVRSLDGVYLTGLNNMAL
                     QLHPDIYEFDRQLRQASATLEAVTPDAAEPTPEELASATPQVNEELLSALKAWRLDRA
                     RRDGVAPFMVAHNTVLEALAAHPPTSPGQLLGVKGMGPAKQQAYGEELMAVIASHVAV
                     SEEATAQAPETPQDPLAETRMAIDRVNRRLLELLKERQDLTDQVAAIKAAHKLAPHQP
                     EREAAMLRDLKHQAGELDLDEELVAALFQLIHDASVGRQTKNTKTK"
BASE COUNT         1263 a         2039 c         2076 g         1576 t
ORIGIN      
        1 tctattcggt gtgttaccga gttgttaacg tgcggtgggg gcctggttgg ccgcttccgg
       61 cgacatcaag cgccagtact tgccgccaac gctgaccgcg acgatatcgc gatccacacc
      121 ggcgtagtct aagaggtgct gctctatagt gatgcggccc tgcttgtcgt ccggctggac
      181 gtcgaccttc cccatacgga attggatgtt caggtcggct acccgttcgt cgaggatgtc
      241 cccttcgagc cgcggttcga ccagattatc ccagacttcc gtcgggtaca ggtgcaagta
      301 tttcccaaag cctcgggtga tgactgctcc gtgggcaaac ttggctcgaa gttcggtcgg
      361 tatggttaac cgccgcttgt cgtcaagctt cctttcgaaa tagtctaccg tggccatctg
      421 ggtcctggtg ttaatggtgc tagtggtttt tttgtttttg tcggtaggct gttttggtct
      481 acctacgatt acccactgcc atcagtctac tacccccttc tacccacaca caatagcaga
      541 actatgaaaa aagtggtgtt tcctacacca cttttccaca cgccgctgcg cttaagtgtt
      601 tatctgttag caatcggact tatgaccggt caaggcactc ggtggcagca atatggtctg
      661 ccctatctgc acctcgttcg gatcgaagcc cttgaaatgc ggcgatttat tttctgcgtc
      721 ggcgatagcg cttgtcatcg ccgatagttg gtcgttgtgt ccagtgtctt ctccgtaacc
      781 ggtggcccgt tccgccacgt ccgtgaggtt ccgctcacca aggcgcaagt gtgtcacgcg
      841 gtggcacgcc gtcgggtcga attcgtacgg gttatgctct ctttcgcagg ccgtcaacag
      901 cagagccgcg cttgccagcg ccagccccgc cccgagcggg ctccgccacc acttattgac
      961 ctctgcaact gattcatcca ccggcgataa ttcggacatg tcgtactccg tacttgttac
     1021 ggtcaaaata ctatactttc gccgtttcgt caatgacggg tgggccctac tgcgctttgc
     1081 gctgcgcacg cgtgatgcgc tcgaccagct gggggttggt cgcataggga ttctctgccg
     1141 gattggcctg gcggcgcatc cgttcgaact gaaggcaggc ccagaagcct tcggccgtca
     1201 cgtactcgcg gtcgaccacc tggtcgccag ccaccacccc ggccgcttcg aggcacaggt
     1261 gggtgatgcg gccggctttc gactcgctgt aggtcaccca gtcctccaga tttgacggga
     1321 gccgggtcaa cctctcgacg tccatgccat gccacagctc gtgcaaagcc acggcacctt
     1381 tcttcagccg gcctgacttc acagtattga tgcccactat catagtcggc cgagccgtta
     1441 tcagcggtcc gttatcgttc agatgctcac ctatggaggt tgtgcgggca tggacccttg
     1501 ccggcatcgc cgcaacattg cgttcgataa gattctgcat atcccaggtt tgcggtgaaa
     1561 tcgcgtcgac cgactgcatg ggcgtaaacc catagcgcgc cgctaagtcg gcatggattt
     1621 tatcgaaccg gtcggccgtc ggcgtaaaca tggacggagc taaccgtatg cccttttttt
     1681 catcttgcga cagtagcgga agaatggcaa aaccattacc tccctcttgg tcgcgcggta
     1741 gctcggccac gccgtgccga acatcgggca gaaaagctcc tgcaatcacc ttctcgtagc
     1801 gctgctgcaa atccggactc aactcgttgt agacatgcgg gtcgtgcagc aattgatcgg
     1861 tggcggtaac gatttcatca aatgcctttg aacgctcacg ctgggctgac ggaatgaatt
     1921 ccataataat tgactagtaa ccggtcgtag cggtggcgcg gagaccgtcg tcgaacttga
     1981 cgggcatgcg gacggtctcg ttcttctcgg caggactgaa gaccacgctc tggttgacca
     2041 cggcttcttt ggtcacccgg ttcagattgc gacgatcatc cccatgaccg aaatcggcca
     2101 tactgaaggc gatcagcgca gtgagagcca gggccccggc ccgctccaag ccggtccggt
     2161 gctgcacttc cagctgttcc agcttgcgca agtcgcggac gacttctttt tctactttta
     2221 ctatttgttt tgtgatgttc acataacaaa cattagcaaa atatttgctt tttgtcaagg
     2281 ttatgcttat ttaggcgtgg agttgttgaa tggcagtcgc cagcgtcgcg gcgtcgtgac
     2341 ggataagcgt gcgactggca gcgatcgcat cccctttccg atccggcgat acgaaatcat
     2401 gcgcgatgag cggtaagccg atggcttcgt acgacgcgac tgagagtttg cggggatcgg
     2461 cctcgaccag cagctcgccg gcgtgaatgt atttgtctaa cactgcttgg tccgggcggt
     2521 cagtgttgta gagcacgtaa tcgagaaccg gcctgcccac gaagcgctcg atttcggcgg
     2581 catagtcatg aactttgaag ccgtcagtct ggccgggctt ggtaaccaga ttgcaagcat
     2641 acaggacggt agcctcggta ctatcgagca cctcggccat gccatccacg atgagtgccg
     2701 gcgccagcga accatacaga ttgccgggag cgataaccac atagtcggcc gccaggatgg
     2761 cgtcttgggc cgcacggctc agatgagccg tcggttccaa tgatagtttg ggccgtgtcc
     2821 ggtcgccgaa atcgaggtta ccgatgcggt actctccgcg gatggtctcc gcgccccaat
     2881 gcaataccaa ttcgctgcga tccgtcgtga cggggacgac tttaccgtgg atacgcagca
     2941 cgtggctggc caactctaca gccgtttcaa aatcctggcc gactttttcg gcggcgctta
     3001 agaataaatt gccgaagctt tgacccttca ggctgccttc gctgaaacgg tagttgaaca
     3061 atgcccgcag ctcgggcgca tcactcagag ccaccagaca ctggcgcacg tcgccgggag
     3121 gtaggacacc tagctcgtcg cgcaagagac cggttgagcc gccgtcgtcg gccatgttga
     3181 ccacggccgt cagaccggtg aaatattcct tgaatctgct gagcagcgtg aaactgcccg
     3241 tgccgccacc gatggtgacg acattgagat gatggatgga agcatcggcc atgaccttaa
     3301 gcgtaacaga aatgacgggt ggcggactat aactcgacga ccatcggcac gccggtctct
     3361 ggcttgagga aggtgatgcc ttggtcagca aacactttcg ccatgccggc atacatgttg
     3421 gcgcgggcat catcgttcca gtgccagtcg tgtatcggga tgatgtagcg cggcttgagc
     3481 tgcagcgcca ggttgaccgc cttcacggtg gcgccccacg gagcggtcac cggtaaggcc
     3541 aggatcggtt tcgtctcgtt gaagctgtgg ctgtcacccg ggtggctgag catgtccagg
     3601 tagtgcacgc cgatctgttc gggcgtggga aacatcggct cgaccgcttc gtgcggcgaa
     3661 tcgaacaacg ccatgccttc gggcggtgtg gtgctggcag ttatgccttc ctggctcagt
     3721 atgtcaacga cttcctgggt ggcggtaatc cgcacctgcg ggaatttggc taccagccgt
     3781 tttatgagat ccatatctat gtggtcgcca tggctgtggg tgatgacgat atcgtcgaga
     3841 tattccagct ggtccacgtc gagggccgga gcgctcatac tgcccgggtc gaacaatacg
     3901 gtgcggttga cgggggccgg catttccacc aacagacagg aatggacgaa tttggtgatt
     3961 ttcatggggc agtctcctct ttcttggatt aatcggacag ggtttgcagg cggcgcaggt
     4021 cgtcgtcggt cagctcaata ccggtggccg cgacattttc ttccaggtga cgcaccgaac
     4081 tggtgccggg aatgggcagc atgatcggtg accgggccag cagccaggcc agcgcgatct
     4141 gctgtggtgt ggcctggtgc gcctcggcga tcgagagcag cgtccgctgg ctgaagtcat
     4201 tcatacctcc acctaccggg taatacggga taaatactat accgtgcgct tcgcaaaaat
     4261 cgacgatcgg atcgctctcc cggcgatggg tgacgttata gtaattctgc acgctgacga
     4321 tttccgtcat ggtgagcgct gtcttaagct gcaacaggct tacattactg agcccgatgt
     4381 ggcggatacg accctcttcc ttgagatcga caagggtttg cactgaggtc tcgaagtcga
     4441 cccgcggatc ggggtgatgg aactgataga gcgtaatctg atcgaccccg aggcgccggc
     4501 aactatcgtc aagtgcctgt cgcaagtgtt ccggcgagcc gtcgagctgc caccggccag
     4561 gtccgctgcg tgtgtagcct cctttggtgg ctatgagcag accttcgtag ggatgtaacg
     4621 tgtcatggat gagctgctcg gacactgccg gtccataggc atcggcggta tcgatgaagt
     4681 tgacgtccag ctgcaccgcc ctcttgagca cgcgccgggc ctccatggga tcgcgtggcg
     4741 gtccccaaat gccttcgccg gtgacgcgca tagcaccgaa gcctaggcgg tttattcgat
     4801 aatgaccgat tttgacgtaa cccgcactca gtgcagacgg tttgctattt gtcatggtag
     4861 ccatatgctc ctcccttttg tttttcgttg tcaggcccga cttatgccct tgaccgaaca
     4921 caagtaacgt cacctattta acagcggcca ccgctcagta gctgtaagaa aattgacagt
     4981 ggcgctgcgc taccggtgat acgctacttt gtttttgtat ttttggtttg cctgccgaca
     5041 ctggcatcgt ggataagctg aaagagtgcc gccacgagtt cttcgtcgag atccagctcg
     5101 ccggcctggt gcttaaggtc gcgcagcatg gccgcctcgc gctccggctg gtggggcgcc
     5161 agtttatgag cggccttgat cgccgctact tggtcggtca ggtcctggcg ttctttgagc
     5221 agttccagca gccgccgatt cacccggtcg attgccatcc gcgtttcggc cagcgggtct
     5281 tgcggcgtct cgggcgcctg cgccgtagcc tcctcgctca cggctacatg ggacgcgatg
     5341 acggccatca attcctcgcc gtaggcctgt tgtttcgccg ggcccatacc cttcacgccc
     5401 agcagttggc cgggcgacgt cggcggatgc gccgccagcg cctcgagcac ggtgttgtgc
     5461 gccaccatga agggagcgac accgtcgcgg cgagcgcggt ccaggcgcca ggccttcagc
     5521 gcgctcagca gctcctcatt gacctgcggc gtagctgacg ccagctcctc cggcgtcggc
     5581 tcggcggcgt caggggtgac ggcttcgagc gtggccgacg cctgccgcag ctgacgatcg
     5641 aattcgtaaa tgtccgggtg cagctgcaat gccatgttgt tcagtccggt caggtacacg
     5701 ccgtctaggc tgcgcacccg gctgagcgcc acgtagccca tgcccggcgt gaacgatttg
     5761 ctgagatcga tctcggcggc gtccaggctc ataccctggc tcttgtggat ggtaatggcc
     5821 caggccagcc gcagcggtaa ttgcacgact tcggcgcgct tgcggtcgtc ttcggtcagc
     5881 gtccagctgt gcggctccac ttttatgatc cgaccgtggg tcagtttgac gagcggcata
     5941 ccgtccttga agccgatcac ctgcccgcgg ctgccgttga cgaagccggc tgcgaaatca
     6001 ttggccacga acatcacctc ggcgccgctt ttgagcgtca gttcttcggg cgccaggacg
     6061 ctgcgagcca gctgctcgac cttggcggcg gcacccttgg tctgcatggc gaaggtgtag
     6121 ccttccgcgc tcagcgcctg cagatgccgc tggttgatgc catcgacatc gatattgtgc
     6181 gtatacaacc gggtgaccgg ttcgtcggcc ggcggtttga cgccgagccg ggactgtaac
     6241 gcctccaggt gcacggtatc cagttcaccg ccgcgcatgg cagtcagcag gtccagtagc
     6301 tcgtcgccgg cctggcggtg ctgctcgctc agataacaga tactcgggtc aagctcttcc
     6361 caggccgccg aggtgtgcgc gaagtcggcc agatcgctca agcggttgac tggcggcagc
     6421 tggaacagat cacccacgag gatgacctgg agaccgccga acggagcgtc gttctcgcgc
     6481 aggattttgc aggcttcgtt gaccatatcg agccgggcgc cgtgcagcat ggatacttcg
     6541 tcgatgatga gcacgtcagt ggcgatgtag cgcttgcgca gccggtcgtt actgcccagc
     6601 cactgcctgt cgttctggtt caggtggtcg cggatgccca ggcccgacca gctgtggatg
     6661 gtcgtcccgc cgatgtgcgt cgccgcgatg ccggtactgg ccgtgatggc cagcgtcttg
     6721 ccggcggctt cggcccgacg gatgtactgg ttgagcacgt acgtcttccc tgcccccggc
     6781 ggcccggtca aaaataccga cttgccggat agcatgatat gtaacgcctg cgcctgcctc
     6841 ataggccctt gattgtagca gcaattgagc cggccgtaaa aaagcggtta tttcttccgt
     6901 ttgcggtcgc ggtacgcgag ggtgcgcttg aactggtcga gccgcacgat gtac
//
LOCUS       DASSFD010000062         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1452874, whole genome shotgun sequence.
ACCESSION   DASSFD010000062 DASSFD010000000
VERSION     DASSFD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452874"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..3366
                     /locus_tag="VFH39_01415"
     CDS             43..3366
                     /locus_tag="VFH39_01415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET6924473.1"
                     /translation="MEDFEQVYAGLNTAQKQAVDAVEGPVLVVAGPGTGKTQLLSARV
                     AQILRVTDALPQNILCLTFTETGASNMRERLTRFIGSDAYDVAISTYHTFGSDIIRRY
                     PEYFTETRLEQPVDELGKRQLLSSIVDRLPYRNPLKQTRHHLGDLIATVSEVKRGLLS
                     PDDLRGIAADNLDAIRHASPDIGAALQAFTSRMPGKLNVAEPAFNAVLQALRSASQKA
                     EPVAPFLGLAQLAAEQLEQALAGTETAGSTKPLTAWKNKWLAKDDANNFVLAGRLEAE
                     RMTALAEVLEAYERLLAERGLYDFDDMILRTIKALENRPELRYSLQEQYQYILLDEFQ
                     DTNAAQLRIVELLTDNPVNEGRPNVLAVGDDDQAIYAFQGAQYSNMLDFFNMYTDVLV
                     VNLSENYRSTGPILETARHLAEQITDSLVSTLPKLTKQLVAAGKNAKAHTSLSHVEYV
                     SDVSERAGVAERIAELMKEGVKPEEIAVLAPKHRYLEALVPYLQDHDIPVSYEHRENI
                     LEAPAIRQLLTMSRLTLALHDRDLTLADSLWPEVLSYEFWGHAVADIWQLSWQAADGH
                     DPWAKLLLDSDRFRGCALLFAALASQATTDPLEIMLDRLIGTEEVQTGDPEQPTIRSP
                     FRDYYTDRSTEDVTLYQVATELAVLRARLREHESQAGDQLTLPSLIDFVDQYQAADQK
                     LINTSPYHQAADAVQLMTVFRAKGLEFEHVFILHAQDNVWGGSAHELGNKLTLPANLA
                     PIRHAGATEDERLRIFFVAASRAKQGLHLTSHRATYAGKQLSRLKYLDEIETENGEVI
                     SRVLPAPYNKVRTDGLEAPALSAMEHDWRTRHLCPDAGLKELLKERLANYRLSPTHLT
                     TFLDLEHAGPQSFLLETLLRFPRAPSTDIAFGNAMHGVLEWIQQQVNAEGSVPSQTAI
                     ERRFRDILTRQRIYGEAYALQEARGLDALRAIVTSPELRFMPGNRPEYTFRQEGVVSG
                     PAVLNGKVDLLEIHKETKTICIVDYKTGSFGTDPAKLHRYTLQLYGYKMLVNGSHSFG
                     GYRVDTAKLVFVDPAMNGGKIVVKPIRFEAAELQRVQKLSEIVWQHIRDLNIPDISSY
                     APTFAAIRQFEQDLLDGKI"
BASE COUNT          785 a         1070 c          995 g          614 t
ORIGIN      
        1 aggcgtgccc aaaccttcgc tggcgtgcca taataggcac atatggaaga cttcgagcag
       61 gtgtatgccg ggctgaatac agcccagaag caggcggtcg atgccgtcga gggaccggtg
      121 ctggtggtgg ccggaccggg caccggcaaa acgcagctgc tcagtgcccg ggtggcacag
      181 atactgcggg tgaccgacgc actgccgcag aacattctct gtttgacgtt caccgaaacc
      241 ggcgccagca acatgcgcga gcggttgacg cgcttcatcg gcagcgacgc ttacgacgta
      301 gccatcagca cgtaccacac cttcggcagc gatatcatcc gccgctatcc ggaatacttt
      361 accgagaccc ggctcgagca gccggtcgat gaactgggca aacggcaact actgagcagt
      421 atcgtcgacc gcttgccgta ccggaacccg ctcaaacaaa ccaggcacca cttgggcgac
      481 ctcatagcca ccgtcagcga agtaaagcgc ggtctgctga gcccggacga cctgcggggc
      541 attgccgccg ataacctgga cgccatccgc cacgccagtc cggacatcgg cgcggctttg
      601 caggccttta ccagccgcat gcccggcaaa ttgaacgttg ccgaaccggc atttaatgcc
      661 gtcttgcaag ccttgcgcag tgcctctcaa aaagctgaac cggtcgcccc gttcctgggg
      721 ttagcgcagc tggctgccga acagctggaa caagcgcttg ccgggaccga gacagccggc
      781 agtaccaagc ccctgaccgc ctggaagaac aaatggctcg ccaaagatga tgccaataac
      841 ttcgtgctgg ccgggcggct ggaggccgag cggatgacag cgctggccga ggtgctggaa
      901 gcctacgagc ggttgctggc cgagcgaggc ctgtacgatt tcgacgacat gatactgcgg
      961 accatcaaag cactggaaaa ccggcccgag ctgcgctaca gcctgcagga acagtaccaa
     1021 tacatactgc tcgacgaatt tcaggacacc aacgccgccc aactgcgtat cgtcgagcta
     1081 ctcaccgaca acccggtcaa cgaaggtcgg ccgaacgtat tggccgtggg tgatgacgac
     1141 caggccattt acgccttcca gggcgcgcag tacagcaaca tgctcgattt cttcaatatg
     1201 tataccgatg tgctggtggt caatctgagc gaaaattatc gctccaccgg cccaatattg
     1261 gagaccgctc gccacctcgc agagcaaatc accgacagtc tcgtcagtac actgccgaaa
     1321 cttaccaagc agctggttgc ggccggaaag aacgccaagg cacacaccag cctgagccat
     1381 gtcgaatacg tcagtgatgt cagtgaacgg gccggggtgg cggaacgtat cgccgaactg
     1441 atgaaggagg gtgtcaaacc ggaggaaatc gccgtgttag cgcccaaaca ccgctatttg
     1501 gaagcgctgg taccatatct gcaggatcac gatatcccgg tcagttatga acaccgcgag
     1561 aacatcctgg aagcacccgc catccgccaa ctgctcacca tgagccgctt gacactggct
     1621 ctacatgacc gcgatcttac cctggccgac agcctgtggc cggaagtcct gagttatgaa
     1681 ttctgggggc acgccgtcgc cgatatatgg cagttgtcgt ggcaggccgc cgacggccat
     1741 gatccgtggg ccaaactact gctggacagc gaccgctttc gcggctgcgc tttgctcttc
     1801 gccgccctgg cgtcccaagc cacgaccgac ccgctcgaaa tcatgctgga ccgactgatc
     1861 ggcaccgagg aagtgcagac tggcgacccg gaacagccga cgatccgttc accgttccgc
     1921 gactattaca ccgaccgctc gaccgaggat gtgaccctgt accaggtggc gacggagctg
     1981 gccgtattgc gggcgcggtt gcgcgagcac gagtcgcaag ccggtgacca gctgaccttg
     2041 ccgtcgctca tcgatttcgt cgaccaatac caggcagccg accagaagct catcaacacc
     2101 agcccgtatc accaggctgc cgatgccgtt cagctgatga ccgtctttcg ggccaaggga
     2161 ctggaattcg agcatgtgtt cattctgcac gcccaagaca acgtgtgggg cggcagtgct
     2221 cacgaactcg gcaacaaact gacgctgccg gccaacctgg cacctatccg ccatgccggc
     2281 gccaccgaag atgaacgctt gcgtattttt ttcgtagccg ccagccgtgc caagcaaggg
     2341 ttgcacctta ccagccaccg ggcgacctac gctggcaaac aattgtccag actgaagtac
     2401 ttagatgaga ttgagacgga gaacggggaa gtcatcagtc gggtgttacc cgcaccgtat
     2461 aacaaggtgc gaacggatgg gcttgaggca ccggcactgt cggccatgga gcatgattgg
     2521 cgaacacggc acctttgccc cgacgccggg ctcaaggaac tccttaagga gcggctggct
     2581 aattaccgac tgagcccgac gcacctaacg acgtttctcg acctagagca cgccggcccg
     2641 cagagcttcc tgctggagac cctgctacgg ttcccgcgcg cgcccagcac cgacatcgcc
     2701 ttcggcaacg ctatgcacgg cgtgctggag tggattcagc agcaggtgaa tgccgagggc
     2761 agcgtgccgt cgcaaaccgc aattgagcgc cgcttccgcg atatactcac gcgacaacgc
     2821 atttacggcg aagcatacgc attacaggaa gcccgcggtc tggatgcgtt gcgggccatc
     2881 gtgaccagcc cggaactgcg cttcatgcca ggcaaccggc ccgaatacac cttccggcaa
     2941 gaaggcgtgg tgagcggacc ggccgtgctg aacggcaaag tcgatctgct ggaaatccat
     3001 aaagagacca aaaccatctg catcgtcgat tacaaaacgg gctcgttcgg caccgacccg
     3061 gctaaattgc atcgttacac cttgcagctg tacggataca aaatgctggt gaacggttcg
     3121 catagtttcg gtggctaccg cgtcgatacc gccaagctgg tgtttgtcga tccagccatg
     3181 aacggtggaa agatcgttgt aaaaccgatc cgtttcgagg ccgcggagtt gcagcgcgtc
     3241 cagaagctaa gcgaaatcgt gtggcagcac atccgcgatc tgaacatacc ggacatttcg
     3301 tcttacgcgc cgacgttcgc tgccatccgg caattcgaac aggacctgct cgacggcaaa
     3361 atataacgcg ccacaccata aaaaaaccgc cgcatgtcct gcggcggttt ttgagtgcaa
     3421 gcaacgatta cttgcgcttg cgggtggtag tcgtcttgcg accg
//
LOCUS       DASSFD010000063         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_21397, whole genome shotgun sequence.
ACCESSION   DASSFD010000063 DASSFD010000000
VERSION     DASSFD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21397"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFH39_01420"
     CDS             <1..275
                     /locus_tag="VFH39_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6924474.1"
                     /translation="FVGSLAYTATDDDLAAFFADAGTVTSAKVIIDRDTNRSKGFGFV
                     EMSSDEEAKAAIDSLNGKDLNGRPVALSEARPKAPRESGSFNGNSY"
     gene            396..941
                     /locus_tag="VFH39_01425"
     CDS             396..941
                     /locus_tag="VFH39_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET6924475.1"
                     /translation="MARLSGKKVAILVDNYFEEAEFTEPLKALQDAGAEVEIIAAEPE
                     DGMVQGLNHVDKAERHKIDKTLDEADIDDYDALVLPGGAVNADHLRMQDKARDWVKNM
                     MDAKKPLAVICHAPWVLASSGIAKGRKLTSYFTIQDDMRDAGADWVDQEVVIDGNLIT
                     SRNPDDLPAFNDALINMMAAA"
     gene            954..1130
                     /locus_tag="VFH39_01430"
     CDS             954..1130
                     /locus_tag="VFH39_01430"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET6924476.1"
                     /translation="MNKYGPKAQEKVEKAMKEFNAGTLRDSHGNKVTKREQAIAIGLD
                     EARREGGKVPTQKK"
     gene            1230..>2481
                     /locus_tag="VFH39_01435"
     CDS             1230..>2481
                     /locus_tag="VFH39_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6924477.1"
                     /translation="MAETVQDPAKIRNIAIIAHVDHGKTTLVDGLLKQSHTFRDNQAE
                     MSQDLIMDSGDQERERGITITAKVTAVQHGDYRINIIDTPGHADFSGEVERTLNMADG
                     CLLIVDAQEGPMPQTKFVLEKALEAELKPIVIINKIDKPGSRIKEVEDELADLFLELA
                     VHEDQLHYPVYYAIGREGKAWAEAPADPTASADLSTIFDAIINKVPAPAVELDKPFQM
                     LVTALAWDNFKGKYAIGRITRGTVKAADQVALCKKDGAVVKTKVELVFMSHGVSKFEV
                     PQGVAGDIVQLTGIADAQIGETVADAEQPEALPTIEVEAPTLRIYLGPNTSPFKGKEG
                     EFNTSRQIAERLKKELETNVGLQVEEDGIGFTVAGRGELHLGVLIESMRREGYEFEVG
                     RPQVVTHQENGQTVEPVEEVIIEVP"
BASE COUNT          636 a          698 c          730 g          417 t
ORIGIN      
        1 tttttgtcgg ttcgctggct tacaccgcta ccgacgacga cctggccgca ttttttgctg
       61 acgccggtac cgtcaccagc gccaaggtca tcatcgaccg cgacaccaac cgcagcaagg
      121 gcttcggctt cgtcgagatg agcagcgatg aagaggccaa ggctgccatc gacagcctga
      181 atggcaaaga cctgaacggc cgcccggtgg ccctcagcga ggcacgcccg aaggctccgc
      241 gggaaagcgg cagcttcaac ggcaactcct actagggtcc cgctctcagc acacaagact
      301 cccggcttga ccgggagtct ttttatgtaa gaaaacgcat agcgcggcgg cccccggcgg
      361 gccatactat atccggtaat aaaaaggagg acataatggc acgattatcc ggtaagaaag
      421 tcgcgatcct ggttgataat tattttgaag aggcagagtt taccgaaccc ctaaaggccc
      481 tacaagacgc tggagctgag gtagagatta ttgctgccga gcccgaggac gggatggtcc
      541 aggggctgaa ccatgtcgac aaggccgagc gtcacaagat agacaagacg ctggatgaag
      601 ccgatatcga cgattatgat gcgctcgtct tgccgggcgg ggcagtgaat gcagaccacc
      661 tgcgcatgca ggataaagcc cgcgattggg ttaagaacat gatggatgcc aaaaaaccgc
      721 tggccgttat ctgtcatgcc ccgtgggtcc tggcctcgag cggtatcgcc aaaggtcgca
      781 aactgactag ctatttcacc atccaggacg atatgcgaga cgccggcgcc gactgggtcg
      841 accaggaggt ggtcatcgac ggcaacctca tcaccagccg caaccccgat gacctaccag
      901 cattcaacga tgcacttata aatatgatgg cggcagccta ggggaggaaa gccatgaaca
      961 agtacggacc gaaagcgcag gagaaggtcg aaaaggccat gaaagagttc aatgccggca
     1021 cgctacgcga cagtcacggt aataaagtga cgaaacgcga acaggcgatc gccatcgggc
     1081 tcgacgaagc gcgccgggaa ggtggcaaag tcccgacgca gaagaaatga ccttgccagg
     1141 aaaacataaa agcctggcag ctgcctaagc tgcgcctaaa ttaggctgta tttttcatcg
     1201 tacctctgtt ataataacgg ttaagaagta tggctgagac agtacaagat ccggctaaga
     1261 tccggaacat tgctattatc gcgcacgtcg accatggtaa aaccacgctg gtcgacgggc
     1321 ttttaaaaca gtcccacacc ttccgcgaca accaggccga aatgagccag gacctcatca
     1381 tggacagcgg tgaccaggag cgggagcgcg gcattaccat caccgccaag gtgacggccg
     1441 tacagcatgg cgattaccgc atcaacatca tcgacacgcc cggtcacgcc gacttttccg
     1501 gtgaggtcga acggacactt aacatggccg acggctgtct gctgattgtg gatgctcagg
     1561 aaggccccat gccgcagacc aagttcgtgc tcgaaaaagc gctcgaggcc gagctgaagc
     1621 cgattgtcat catcaataaa atcgacaaac cgggcagccg tatcaaagaa gtggaagacg
     1681 aactggccga cctgttcctg gagctggcgg tgcacgaaga ccagctgcat tacccggtgt
     1741 actacgccat cggccgggaa ggtaaggcgt gggccgaagc accggctgac cccacagcca
     1801 gtgccgattt gtcgacgatc ttcgacgcca tcatcaataa ggttccggcc ccagccgtcg
     1861 agctggataa gccattccag atgctggtga cggcgctggc gtgggacaac ttcaaaggca
     1921 aatacgccat cggccgcata acccgcggca cggtcaaagc cgccgaccag gtggctttat
     1981 gcaaaaagga cggcgctgtc gtgaagacga aggtcgaatt ggtgttcatg agccacgggg
     2041 tcagcaagtt cgaggtgccg cagggcgtag ccggcgacat cgtgcagctg accggcatcg
     2101 ccgacgccca gatcggcgag accgtcgccg acgccgaaca gccggaagca ctgccgacca
     2161 tcgaagtcga agcaccgaca ctgcgtattt atctcggccc gaacaccagc ccgttcaaag
     2221 gcaaagaagg cgagttcaat acttcccgcc agatcgcaga gcgcctcaag aaagagctcg
     2281 agacgaacgt gggcctgcag gtcgaggaag acggcatcgg cttcacggtg gccggacggg
     2341 gtgaactgca cctcggcgtg cttatagaaa gcatgcgccg agaaggttat gagttcgaag
     2401 tcggccgccc gcaggtcgtc acccatcagg agaacggtca gacggttgag ccggtagaag
     2461 aagttatcat cgaggtaccg g
//
LOCUS       DASSFD010000064         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_289611, whole genome shotgun sequence.
ACCESSION   DASSFD010000064 DASSFD010000000
VERSION     DASSFD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289611"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFH39_01440"
     CDS             <1..810
                     /locus_tag="VFH39_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6924478.1"
                     /translation="VDSVGGITVNVPEQLYDPTMAWENHWNPVLAKAGVQQMNGKQAL
                     LYARSRETSSDFARGERQRAIILALKEKAMTLGTLSNPLKISQLMSAFGDNMVTDLSL
                     SDAVRLYDITKDISNDHVQSLDLVTPPHTLITTGNMHGVSIDQPKAGIGNYGDIQAYV
                     RSALKDGFLAKENAQLTVLNGTTVAGMATQKGNELKSYGYNVVKVDNAPTDSYDKTVI
                     VDLTNGQDKYTRHYLENRFGVTAVTSVPDPNIKPGTSNFIVILGQDQVNRN"
     gene            894..1655
                     /locus_tag="VFH39_01445"
     CDS             894..1655
                     /locus_tag="VFH39_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924479.1"
                     /translation="MKPIVDRLRPASGVSYVLHLVLLVLLPLVAYVLVQLGDTFVKLA
                     YAVVLLSKWRMFAVRPRFWPAIIRANSVDIMVGLSIVLFMTHAGSQWLQLLWAALYAV
                     WLIVIKPATGSIMTSLQAMIGQLLGLSAVYLTWSAAPMWVLVLATGLVCYLSARHFFD
                     SFDEVYSRLLAYLWGYFGAALAWLLSHWLLFYAHGLVAQPTLILTVLGYGLAALYHLD
                     HEERLSLTLRRQFVFVMVAVILIILTFSDWGDKVI"
     gene            1770..>2512
                     /locus_tag="VFH39_01450"
     CDS             1770..>2512
                     /locus_tag="VFH39_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6924480.1"
                     /translation="MKPKDASSGSTKTPEYSVSSRTPDDSWNFSIPKVKLPRSQMSSG
                     WRGPKNAAVAVLLVAVLLVGFLGGWLGAAVHSNSDSQTASLGNSLKVVTSQSQLIDDI
                     AKTVGPSVVSVDVVSQTTDNSPNNFFDYFGYGGGSGGTTQQESAGTGIILSSDGLIMT
                     NRHVVPAGSTSVSVTLSDGTQLNNVQVLGRTSESDSLDVAFLKITDAKNHKLTPVALG
                     NSSKVQVGDAVVAIGNALGQFQNTVTSGI"
BASE COUNT          510 a          747 c          764 g          491 t
ORIGIN      
        1 gtcgatagcg tcggcggtat caccgtgaac gtccccgagc agctgtacga cccgaccatg
       61 gcgtgggaga accactggaa cccggtgctg gcgaaagccg gcgtgcaaca gatgaatggc
      121 aagcaggcgc tgctgtacgc ccgttcccgc gagacatcgt ccgacttcgc ccgtggggaa
      181 cggcagcggg ccatcatatt ggcgctcaaa gagaaggcca tgacgctcgg tacgctgagt
      241 aacccgctga agatttccca gctcatgagc gccttcggcg acaacatggt gaccgatctg
      301 agcttgtccg acgccgtccg gctgtacgac atcaccaaag acatcagcaa tgaccacgtg
      361 caatcgctgg acctggtgac accgccgcac acgctcatca cgaccggcaa tatgcacggg
      421 gtttccatcg atcagccgaa ggccggcatc ggcaactacg gcgacatcca ggcctatgtg
      481 cgctcggcgc tcaaagacgg cttcctggcg aaagaaaacg cccagctgac ggtcctgaac
      541 ggcaccaccg tcgccggtat ggccacccag aagggcaacg agctgaagtc gtacggctac
      601 aacgtggtga aggtcgacaa cgcgccgacc gacagttacg acaagacggt cattgtcgac
      661 ctgaccaacg gccaggacaa gtacacccgc cactacctcg aaaatcgctt cggcgtcaca
      721 gccgtcacct cggtacccga cccgaatatc aaaccgggaa cgtccaactt catagtcatt
      781 ctcggtcagg accaggtcaa ccggaactag cggcccgtag agcggcttcg gtgcagggcg
      841 gttttgcccc cgacatggta tgattaaaga aagcgagtta ttgaggtaag gtaatgaaac
      901 cgatcgtcga cagattgcgc ccggccagcg gtgtgtcata tgtgctgcac ttggtgctgc
      961 tggtgctgtt gccactggta gcctatgtat tggtgcagct cggcgatacg ttcgtgaagc
     1021 tggcctacgc cgtggtgctg ttgagcaagt ggcggatgtt cgccgtgcgg ccgcgtttct
     1081 ggccggctat cattcgcgcc aactcggtcg acatcatggt cggcctgagt atcgtactgt
     1141 tcatgacgca tgccggttcg cagtggctgc agctgctgtg ggcggcgctg tacgcggtat
     1201 ggttgatcgt catcaagccg gctacaggca gcatcatgac cagcctgcag gcgatgatcg
     1261 gccagctgct gggcttgagt gcggtgtatc ttacctggtc agccgcaccg atgtgggtgc
     1321 tggtgctggc caccggcctg gtgtgctacc tcagtgcccg gcacttcttc gacagcttcg
     1381 acgaagtcta tagccggctg ctggcctacc tgtggggata cttcggggcg gcgctggcct
     1441 ggctgctgag ccactggctg ttgttctacg cccatggtct cgttgcccaa ccgacgctta
     1501 tcctgacagt actcggttac ggcctggcgg cgctgtatca cctggatcac gaggagcggc
     1561 tcagtctgac gctgcggcga caatttgtgt tcgtcatggt cgccgtcatt ctcatcatcc
     1621 tgaccttctc cgactggggc gacaaagtca tctagctagc ggtacgtctc ttggcgataa
     1681 tcaatcgccg tcggttctca ggttcgttac agccgcctcc ggtgtaattg tgtttgacgt
     1741 ataatggaag cataagcgga gggagaaaag tgaaaccgaa ggatgcctcg tccggatcaa
     1801 ccaagacgcc tgagtacagc gtgtcgtcac gcacgccgga cgacagctgg aatttcagca
     1861 taccgaaagt gaaactgccg cggtcgcaga tgtcatccgg ctggcgcggt cccaagaatg
     1921 ccgccgtcgc cgtgttgctg gtggccgtgc tgttggtcgg cttcttgggc ggctggctcg
     1981 gcgctgcggt tcactcgaat agtgacagcc aaacggccag cctcggcaat tccctcaagg
     2041 tcgtgaccag tcagagccag ctgatcgacg acatcgccaa gacggtcggc cccagtgtcg
     2101 tctcggtcga tgtggtgagt cagaccaccg acaacagtcc caataatttc ttcgactact
     2161 tcggctacgg gggcggtagc ggcggtacga cgcagcagga aagcgccggc accggcatca
     2221 tcctgagcag cgacgggctt atcatgacca accggcacgt cgttccggcc ggcagtacca
     2281 gcgtgagcgt cacgctcagc gacggtacgc agctgaacaa tgtgcaggtg cttggccgta
     2341 ccagcgaaag cgacagcctc gatgtggcat tcctgaagat caccgatgcc aagaatcaca
     2401 agctgacgcc ggtggcgctg ggcaactcgt cgaaggtgca ggtcggtgac gccgtggtgg
     2461 ccatcggcaa cgccctgggc caattccaga acaccgtcac cagcggcatc at
//
LOCUS       DASSFD010000065         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_999571, whole genome shotgun sequence.
ACCESSION   DASSFD010000065 DASSFD010000000
VERSION     DASSFD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999571"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1300
                     /gene="rsmH"
                     /locus_tag="VFH39_01455"
     CDS             386..1300
                     /gene="rsmH"
                     /locus_tag="VFH39_01455"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET6924481.1"
                     /translation="MHKSPNKNQHQSNLHQPVLLDEVLDCLAPAKGERYLDLTAGYGG
                     HAAAILEYTHGAAVLVDRDENAIRELTSRLPGADIRHDDFYSASQQLAVEKQHFDMIL
                     ADLGVSSPHLNEATRGFAINQEGPLDMRMDPRQELTAETVVNTYGEADLADILWRYGE
                     EPKARQIARAIVANRPLATTSQLAAIVAKAWPGRSRVHPATRTFQALRIAVNDELGLL
                     ERSLPIWTSLLASGGRLAVISFHSLEDGIVKRFLQERAGDRYDAELRLITKRPVTASP
                     HELVSNPRARSAKLRAAAKINIQKERER"
     gene            1458..1805
                     /locus_tag="VFH39_01460"
     CDS             1458..1805
                     /locus_tag="VFH39_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924482.1"
                     /translation="MTYSSSLAMSRSRFTVRNQNSVSYRVEERRLGPISNTIVLIVLA
                     CLLGLLYLTQVTKTNAYGYQINSLQQQSASLQTKHDDLEVASARLQALPRVQSSAVAQ
                     SMTPVSPSDTVQN"
     gene            1875..>2994
                     /locus_tag="VFH39_01465"
     CDS             1875..>2994
                     /locus_tag="VFH39_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET6924483.1"
                     /translation="MLTNQAPAAHAVKRVRTWYTLLILVIAVFGVRLFYLQIIRYDHY
                     KKAALADQLKQYQIPASRGLIEAYDGSGNVVPIVLNQQLYTLYADPTYIKHPQAVADK
                     VAGIIGGKASDYLSGLTAKDTRYQILAKRITPDQQKKIVALQYPGLGTQEQDYRTYPQ
                     GALAAQVLGFVNDAGEGVYGIEQDQNKLLAGTPGELKAITDLNGVPLAASNGNIETKP
                     VAGENVVLTLDIGMQQQLENILAREYKATKSKGISAVVMDPNTGQVKAMANYPSFDPA
                     HYQNVNDAKVFQNGAVTEAIEPGSTMKLLTTAAALDQGVINPNTTFYDPAHWIVDGFN
                     ITDIEEDGGARTQSIASTLNLSLNTGATWMLMQMGGGQI"
BASE COUNT          800 a          875 c          788 g          531 t
ORIGIN      
        1 ctctatagac tgggaagagc aggcacggtt tctgcgatag cagaaattcg gatttattcc
       61 gaaagtgccg ttgagaaacc cgctatgcgg gtgtctcaag cgtacctatt cggcagtcag
      121 caagtggtaa cagggcacca ctttaaaaac cctcgtacct ttcttaggaa aacacctccc
      181 aaaactccac ctcacacata agtctctcaa acacgctaca tacggttaag aagcaacggt
      241 ttatccagct tcccaaactg gttcgtagca acaaaaacaa aaaccccaaa aaaacaaaaa
      301 gctgactgct gattaggtgc tcttgaacca agtcgaacca ataactgtga agactgaaaa
      361 caaacaccaa aacaaaagtg tgccgatgca caaaagtcca aacaaaaacc aacatcaatc
      421 aaatctccat caaccggtcc tgctagacga agtcctggat tgcctggccc ctgcgaaggg
      481 cgaacgctat ctggatctga cggcaggcta tggcggacac gccgcagcca tactggaata
      541 cacccacgga gcagccgtcc ttgtcgatcg cgacgagaac gccatccggg agcttacctc
      601 caggttgccg ggcgccgaca tccggcacga tgatttctat agcgccagcc agcagttggc
      661 cgtcgagaag cagcactttg atatgatact ggccgatctg ggcgtatcgt caccgcacct
      721 taacgaggca acccgagggt ttgcaatcaa ccaggaaggt ccgctcgata tgcggatgga
      781 tccgcgtcag gaattgacag ccgaaacggt ggtcaataca tacggagaag ccgatctggc
      841 cgacatactg tggcgttacg gcgaagaacc gaaagcgcgg cagatagcca gggcaatcgt
      901 ggccaaccga ccgcttgcta ccaccagcca gttggcggcc atcgtcgcca aagcttggcc
      961 aggacgcagc cgtgtccatc cggcgaccag aaccttccag gcgctccgta tcgccgtcaa
     1021 cgacgaactg ggcctgctgg aacgcagcct gccgatctgg acgagcctcc tggcctccgg
     1081 cggacgattg gcagtcatca gcttccatag tctggaagac ggcatcgtca aacgctttct
     1141 gcaagagaga gcgggtgacc gctacgacgc cgagctccgg ttgattacca agcgacccgt
     1201 gaccgcctca ccccatgaat tagtttccaa tccgcgtgcc agaagcgcta agctccgggc
     1261 cgcagcaaaa ataaatatac aaaaagaaag ggaacgctag cgatgcctat ccacgtcaaa
     1321 agcagctccc gggcctacaa agtccacgtc aaagtggtct aagtacccga aagcaaatcg
     1381 ggaagggtgc caaaatacgc acccttccca actaaaaatc aaaaagacaa acataaaaac
     1441 aaaagagtaa gtagtacatg acatattcaa gttcgctcgc gatgagccgc tcgcgcttta
     1501 cggtgcgcaa ccagaactcg gtgagctatc gcgtcgaaga acgccgccta ggacccatca
     1561 gcaataccat cgtgctgatc gttctggcat gtctgctcgg acttttatac cttacccagg
     1621 tgaccaagac caacgcctac ggttaccaga tcaactccct ccagcagcag tccgccagct
     1681 tacagaccaa gcacgatgac ctggaagtcg cttctgcccg tctgcaggct ttgccgcgcg
     1741 tccagagcag cgccgtcgcc cagtcgatga cgccggtttc gccgagcgac accgtccaga
     1801 actaactaga agcgctccgc ttaagaggta ccaggcgaca aaactccatt ggctgaggta
     1861 taatcataat caatatgctg acaaatcaag caccggcagc ccatgccgta aagcgtgtcc
     1921 gtacctggta cacgttgctt atccttgtca ttgcggtgtt cggcgtgcga ctgttttacc
     1981 ttcagatcat ccgttacgac cactataaaa aagcggccct ggccgatcaa ttgaagcagt
     2041 accagatacc cgccagccgg gggctgatcg aagcgtacga cggcagcggc aacgtggtgc
     2101 ccatcgtctt gaatcagcaa ttgtacacgc tgtacgccga tccgacctac atcaagcacc
     2161 cgcaggccgt ggccgacaaa gtggcaggca taatcggcgg caaagcttcg gactatctgt
     2221 ccgggctgac ggctaaagat acgcgctacc agattcttgc caaacggata acgccggatc
     2281 agcagaaaaa aatagtcgcc ttgcagtacc cggggctggg cacgcaggag caggattacc
     2341 gaacgtaccc gcagggcgcg ttggctgcgc aggtgctcgg cttcgtcaac gacgccgggg
     2401 aaggggtgta tggtatcgaa caggatcaga acaagctgct ggctggtacg ccgggtgagc
     2461 tgaaggctat caccgatctg aatggtgtgc cgctggccgc cagcaacggc aacatcgaga
     2521 ccaagccggt ggccggcgaa aacgtggtgc tgacgctcga tatcggcatg cagcagcagc
     2581 tggaaaacat actggctcgc gagtacaagg cgaccaaatc caaaggtatc agcgccgtcg
     2641 tcatggaccc gaatacgggg caggtgaagg ccatggccaa ctatccgtcg ttcgatccgg
     2701 cccattacca gaatgtcaac gacgccaaag ttttccagaa cggggcggtg accgaagcta
     2761 tcgagccggg gtcgaccatg aaactgttga ctacggccgc agcactggac cagggggtca
     2821 tcaacccgaa caccactttt tatgacccgg cccactggat agtggacggg ttcaacatca
     2881 ccgacatcga agaggacggc ggtgcccgca cccaaagtat cgccagcacg ctgaacctca
     2941 gtctgaacac cggcgcgacc tggatgctga tgcagatggg cggcggccag ataa
//
LOCUS       DASSFD010000066         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1430935, whole genome shotgun sequence.
ACCESSION   DASSFD010000066 DASSFD010000000
VERSION     DASSFD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430935"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFH39_01470"
     CDS             <1..835
                     /locus_tag="VFH39_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924484.1"
                     /translation="PVIVSGGWTWEEWQGNATAQHPVDFAYYGTEKKAGNARGLPNSG
                     REGLRQLGPMIRRLKEQGIKTGIQITNLPFERPADVIPQLAELAAEQEPAFVEVNLSC
                     PNGKRPDGGLEPALSENAEACGEVMGLAYEKIGGAVLLGAKDSPHVASPRGMIDHQKV
                     FDVVGAVKPYIDFLTGTNTIGNQDFSELTCADGKGGMSGPAIAPVARDWLMVAREALS
                     PDIPILSCGGIDSENVAKETRERQILGALLVGGAQEFYRKRPVQVALQWAERWLLSDN
                     S"
     gene            complement(819..1313)
                     /locus_tag="VFH39_01475"
     CDS             complement(819..1313)
                     /locus_tag="VFH39_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET6924485.1"
                     /translation="MATFSFDVVSEYDKAEMNNVFDQVQRELQNRYDFKGTPAAVDWL
                     DDKKGLKITGNGDWQIDAILDIVRKKLAARGQSQKVLDTTREAVESNLKTTKEVPFKE
                     GLDQDKAKKITSLVRDKLPKVKTQIQGDAVRVMSGSKDDLQQVIQLLKAADFDYPLSF
                     TNYR"
     gene            1379..1717
                     /locus_tag="VFH39_01480"
     CDS             1379..1717
                     /locus_tag="VFH39_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924486.1"
                     /translation="MTKMLERPVAETAELTPLEAGARALVTLYFQRDDTWVPADRWSG
                     EVRPNPQCYEADDSYVNVLPDEPRGTEPYGSRHPRLMNRTEPFRHEFANGEAVWLRYG
                     VELDLPRSAE"
     gene            1852..>2585
                     /locus_tag="VFH39_01485"
     CDS             1852..>2585
                     /locus_tag="VFH39_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924487.1"
                     /translation="MQKTLYHLGLITSSLFAMAVLAVAPVAADQGSGGASSDDSLSTT
                     SQTQIGSDNGSVKTEFHAHPLSNSDEQVALTTSETESGSSDNGHHSLRAEAEALLNQK
                     RQNGKEHSAAQRKTACEAHAAEINTRSQNYAAAAQRHLDVFNSIFTKVQAFQTSKQLN
                     VSNYDSLVATATAKQTAAQSAVDALKALDVSIDCSSTDPAATVATLKEAVINARTTLQ
                     DYRSAIKDVIVALKGASTANTTTDTTT"
BASE COUNT          548 a          741 c          769 g          527 t
ORIGIN      
        1 gccggttatc gtatcaggcg gctggacctg ggaggagtgg cagggcaacg ccacggctca
       61 gcacccggtt gattttgcgt attatggaac agaaaagaag gccggcaacg cgcgtggtct
      121 gccaaatagc ggtcgcgaag ggcttcgtca gctcggtcct atgattcgac gcttgaaaga
      181 gcagggcatt aagaccggta tacagataac caatctgccg tttgagcggc cggcggatgt
      241 tataccacag cttgcggaac tggcggccga gcaggagccg gctttcgtcg aggtgaatct
      301 gagctgcccg aacggcaaac gccctgacgg cggtctggaa ccggcactct cggaaaacgc
      361 cgaggcgtgt ggcgaggtaa tgggactggc ctacgaaaag atcggtggcg cggtgttgtt
      421 gggcgccaaa gactcgccgc atgtcgctag cccgcgcggt atgatcgatc atcagaaggt
      481 gttcgatgta gtaggagccg ttaagcccta cattgatttc ctaacgggga ctaataccat
      541 cggtaaccaa gacttttctg agcttacgtg cgctgacggt aagggcggta tgagcggtcc
      601 ggctatcgct cccgtcgccc gcgattggtt aatggtggcg agggaggccc tctctcctga
      661 catacccatc ctgtcgtgcg gcggaatcga tagcgaaaac gttgccaaag aaacgagaga
      721 gcggcagata cttggagcct tacttgtcgg gggtgcccag gaattctatc ggaagcgccc
      781 cgtgcaggtc gctttgcaat gggcggagcg gtggttgctt agcgataatt cgtgaagctg
      841 agcgggtagt cgaaatcggc cgccttcagc agctgaatga cctgctgcag gtcgtcctta
      901 ctgccgctca tcacccgtac cgcgtcgccc tgtatctgcg tcttcacctt cggtaatttg
      961 tcgcgtacca gcgaggtgat cttcttggcc ttgtcctggt cgagcccttc tttgaacggc
     1021 acttcctttg tcgtcttcag gttagactct accgcctcac gggtggtgtc gagcactttc
     1081 tggctctgcc cgcgggcagc caacttcttg cggacgatgt ccaggatggc atcgatctgc
     1141 cagtcgccgt taccggttat cttcagcccc tttttgtcat ccagccagtc gacggcggcc
     1201 ggtgtacctt tgaagtcgta gcggttttgc agttcgcgct gcacctggtc gaagacgttg
     1261 ttcatctcag ccttgtcgta ctcgctcacc acgtcgaaag aaaatgttgc cataccatgt
     1321 agtgtagcaa agtcacggtt gaccggccgg tgcgccgagg cgtttaatca taagtaagat
     1381 gacaaaaatg ctggagcggc cggttgccga aactgccgag ttaacgccac tagaggctgg
     1441 tgcccgtgca ttggtaaccc tgtattttca gcgtgatgat acgtgggtac cggccgacag
     1501 atggtcggga gaagtacggc cgaacccgca gtgctacgaa gccgatgaca gctacgtgaa
     1561 cgtgttgccc gacgagcctc gaggcaccga accgtatggc tcacgccatc cccgcctcat
     1621 gaaccgtacc gaaccgttcc gccatgagtt tgctaacggc gaggcggtct ggctccgtta
     1681 cggcgttgaa ctcgatttgc cccgtagtgc agaatagcct cgctgtcttc gcgaagtgtt
     1741 ccttgacgta tccgcccaca agcggtgcgc tgcagcggct ctgccgttgg gttgcgctgc
     1801 aaagcgctcg cgcttatagt gagtagcacg gtaaaaaaag gaatatctcg catgcaaaaa
     1861 acactttatc atctcgggct cattacgagc tcgttgttcg ccatggccgt gctggccgtg
     1921 gctccggtcg cggcagacca aggctccggc ggcgcgtcca gcgatgacag tttgagtacg
     1981 acctcgcaga cacagatcgg atcggataac ggcagcgtga agacggagtt ccatgctcat
     2041 cccctctcca actcggacga gcaggtggcg ctgacgacca gcgagaccga atcagggagc
     2101 agcgataatg ggcaccacag tctgcgggct gaagcggaag cgttactgaa ccaaaagcgc
     2161 cagaacggca aagagcacag tgccgctcag cgcaagacgg cttgcgaagc gcacgccgcc
     2221 gagatcaata cccgcagcca gaactatgcc gcggcagctc agcgccacct tgatgtcttc
     2281 aacagcatct tcacgaaggt tcaggccttt cagaccagca agcagttgaa cgtcagtaat
     2341 tacgacagtc tggtggcgac cgccacggca aagcagacgg ccgcccagag cgccgtcgat
     2401 gccctgaagg cgctcgatgt cagcattgac tgctcctcga ccgacccggc ggcgaccgtc
     2461 gccacactta aagaggcagt catcaacgcc cgcacgacgt tgcaggatta ccgcagcgcc
     2521 atcaaggatg tcatcgtggc gctcaagggt gcctcgacgg ccaacactac caccgatacc
     2581 accac
//
LOCUS       DASSFD010000067         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_522302, whole genome shotgun sequence.
ACCESSION   DASSFD010000067 DASSFD010000000
VERSION     DASSFD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522302"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /locus_tag="VFH39_01490"
     CDS             complement(<1..2508)
                     /locus_tag="VFH39_01490"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET6924488.1"
                     /translation="MSQTSSLGVTRLFTKPGTKAYDQLKWAKRDSIITNPGTGKNVFE
                     QKGVEFPQGWSLNAINIVAQKYFTGTPGTKERESSLKHLIDRVVDTVTRQGLQEGYFE
                     DSEAEDFREELKYILATQRAAFNSPVWFNIGAPERSQQASACFILKVEDTMASILNWY
                     TEEGMIFKGGSGSGLNVSAIRSSKEALGKSAGTASGPLSFMRGADASAGAIKSGGKTR
                     RAAKMVIMNVDHPDVEDFIWCKAIEERKARVLQEAGFDMNLDGKDSFSVQYQNANNSV
                     RVTDDFMKAVENDDTWDLKAVTNGKTIKTVKARELFRQFAEAAWECADPGMQFDTVIN
                     KWHTTPNAGRINGSNPCSEYMHLDNSACNLASLNLLKFLNDDGSFDIKSFIHTVELIF
                     TAQEILVGYSEYPTENITKNAKAYRELGIGYANLGALLMAKGLPYDSDEGRALAAAIT
                     ALLTGQAYATSAKLARRVGPFAGFHKDRDGMLNVLRMHRAAVNDIDVSLVSEELLSAA
                     AQSWDEAVELGEQFGVRNSQASVLAPTGTIGLMMDCDTTGIEPDLGLVKFKKLVGGGS
                     MTIVNQTVPRALKALGYSKQQIDDIVNYIDTEKTITGAPHLKDEHRAVFSCSMGDNPI
                     HYRGHVRMMAAVQPFISGAISKTVNMPEDVTVEDVEQIHLESWKLGLKAVAIYRDNCK
                     VAQPLSMLKKGGDANNEAPAAAPATAEPQVVEKVIVKGPARRTLPRVRNSRTYRFVIA
                     DLEGYFTVGEFEDGTPGELFINVSKQGSTLSGVMDSLAISISQGLQYGVPLKRYVKSF
                     RGSSFAPAGITDDVDIRTASSITDYIFRRLALDYLSFD"
     gene            complement(2711..3184)
                     /gene="nrdR"
                     /locus_tag="VFH39_01495"
     CDS             complement(2711..3184)
                     /gene="nrdR"
                     /locus_tag="VFH39_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET6924489.1"
                     /translation="MKCNQCHFDDTKVIESRDVADGQAVRRRRLCPSCSNRFTTYERI
                     ERPQLIVVKNDGTRELFNRSKLLNGLYHACEKTPVTSLQLERLVGDIEQELYACAEGE
                     VSSAKVGDMVMARLAALNEVAYVRFASVYRRFKDIAGFERELSNIKNQKAQPIGN"
BASE COUNT          569 a          967 c         1084 g          805 t
ORIGIN      
        1 gtcgaagctc aagtagtcca aggccagacg acggaagatg tagtccgtga tgctgctggc
       61 ggtgcggatg tcgacgtcgt cggtgatacc ggcgggcgcg aagctggagc cgcggaagct
      121 cttgacgtag cgcttcagcg ggacgccgta ctggaggccc tggctgatgc tgatggccag
      181 gctgtccatg acgccactga gagtactgcc ctgcttgctg acgttgatga acagttcacc
      241 cggggtgccg tcttcgaact cgccgacggt gaagtaacct tcgaggtcag cgatgacgaa
      301 gcggtaggtg cggctgttgc ggacgcgcgg cagggtgcgg cgggccggac ccttgacgat
      361 taccttctcg acgacctgcg gctcggcagt cgccggtgcg gcggccgggg cttcgttgtt
      421 ggcgtcgccg cccttcttga gcatgctgag cggctgcgcg accttacagt tgtcacggta
      481 gatggcgacg gccttcagac cgagcttcca cgattcgagg tggatctgct cgacgtcttc
      541 gaccgtgacg tcttccggca tgttgacggt cttagaaatg gcaccggaga taaacggctg
      601 cacggcggcc atcatgcgga cgtggccgcg gtagtggatg gggttgtcgc ccatgctgca
      661 gctgaagacg gcgcggtgct cgtccttcag gtgcggcgcg ccggtgatgg tcttctcggt
      721 gtcgatgtag ttgacgatgt cgtcgatctg ctgcttgctg tagccgagcg ccttcagggc
      781 acgcggcacg gtctggttga cgatggtcat actaccgccg ccgaccagct tcttgaactt
      841 gaccagaccc aggtccggct cgatgccggt ggtgtcgcag tccatcatca ggccgatggt
      901 accggtgggg gccagcacgg aagcctgtga gttgcggacg ccgaactgtt cgcccagttc
      961 aaccgcttcg tcccaggact gggcggcggc actcagcagc tcctcggaga cgaggctgac
     1021 atcgatgtcg ttgactgcgg cacggtgcat gcgcagcacg ttgagcatgc cgtcgcggtc
     1081 cttgtggaag ccggcgaacg gaccgacccg gcgggccagc ttggcgctcg tggcgtacgc
     1141 ctggccggtt aagagcgcgg tgatggcggc agccagggcg cgaccctcgt cgctgtcgta
     1201 cggcagcccc ttggccatca gcagtgcgcc caggttggcg tagccgatac cgagctcgcg
     1261 gtaagccttg gcgttcttgg tgatgttctc ggtcgggtat tcgctgtaac ctaccaggat
     1321 ttcctgcgcg gtgaagatca gttctaccgt gtggatgaag ctcttgatgt cgaagctgcc
     1381 gtcgtcgttc aggaacttca acaggttgag gctggccagg ttgcaggccg agttgtcgag
     1441 gtgcatgtac tcactacatg ggttggagcc gttgatgcgg ccggcgttcg gcgtggtgtg
     1501 ccacttgttg atgacggtgt cgaactgcat gccggggtcg gcgcactccc aggcggcttc
     1561 ggcgaactga cggaacagct cacgggcctt gaccgtcttg atggtcttgc cgttggtgac
     1621 ggccttgagg tcccaggtgt cgtcgttctc gacggctttc atgaagtcgt cggtgacgcg
     1681 taccgagttg ttggcgttct gatactggac gctgaagctg tctttgccat ccaggttcat
     1741 atcgaaacct gcttcctgta ggacgcgcgc cttgcgctcc tcgatcgcct tgcaccagat
     1801 gaaatcctcg acgtccgggt ggtcgacgtt catgatgacc atcttggcgg cgcggcgtgt
     1861 cttaccacca ctcttgatgg cacccgccga agcgtcggcg ccgcgcatga agctcaaagg
     1921 accgctggcc gtgccggcgc ttttgcccaa agcttccttg ctactgcgga tggcgctgac
     1981 gttcaagccg gaaccggagc cacccttgaa gatcatgcct tcttcggtgt accagttcag
     2041 gatgctggcc atggtgtctt cgaccttcag gatgaagcag gcgctggcct gctgcgagcg
     2101 ctccggcgca ccgatgttga accacaccgg gctgttaaaa gcggcacgct gcgtggccag
     2161 gatgtacttc agctcttcgc ggaagtcctc ggcctcgctg tcttcgaagt agccttcctg
     2221 cagaccctgg cgcgtcacgg tgtcgacgac gcggtcgatc aggtgcttca ggctgctctc
     2281 acgctctttg gtaccggggg tgccggtaaa gtatttctga gccacgatgt tgatagcgtt
     2341 cagactccag ccctgcggaa actcgacgcc cttctgctcg aagacgttct tgcccgtccc
     2401 cggattggtg atgatgctgt cgcgtttcgc ccacttaagc tggtcgtacg ccttggtacc
     2461 aggcttggtg aacaggcggg tgacgcctag actgctggtt tgactcatag ttctaccctc
     2521 actttttagt tgttattagt tttttaggtg tttgtttaga gtggccgcgg atcatatggc
     2581 tctcaaaaca tatgatcatt tggtgcgctc ccccaggact tcaatcgttt gagatcccgc
     2641 ggcagccacc aatcaggcct tagagcgacg ttggtacaag gtaccacgtc gccccggtga
     2701 cgtacaaatg tcagttgccg atcggctgtg ccttttggtt cttgatgttg ctgagctcgc
     2761 gttcgaaccc ggcgatatct ttgaaccggc ggtacacact cgcgaagcgc acgtaggcga
     2821 cttcgttgag cgcggccaac cgcgccatta ccatgtcgcc caccttggcg ctgctgactt
     2881 cgccctcggc gcaagcgtac agctcctgct cgatgtcgcc caccagtcgt tccagttgca
     2941 aactggtgac cggcgtcttc tcgcaggcgt ggtacaggcc gttcagcagc ttgctgcggt
     3001 tgaacagctc ccgtgtgccg tcgttcttga cgacgatcaa ctggggccgt tcgatgcgct
     3061 cataggtggt gaaccggtta ctgcagctcg ggcacaggcg gcggcggcgg acggcttggc
     3121 cgtcggcaac gtcacgtgat tcgatgacct tggtgtcatc gaagtgacat tggttacatt
     3181 tcatgtccct acctccatga gtctacggtg tcaggcggcc acgaaactgg catttgaaac
     3241 aacaagtgtt tgcaataccc gtcgcggatt cggctgatat ttgtagactt ttttgttagt
     3301 taatgtgcac tcggctggtg ggttagggcc cacatatagc gcttagtgat gttagtctaa
     3361 ctctagctgt agcggtttgc aatagaattg ccgctgtatt atttagtaca ggacttattt
     3421 cgtct
//
LOCUS       DASSFD010000068         6933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1311948, whole genome shotgun sequence.
ACCESSION   DASSFD010000068 DASSFD010000000
VERSION     DASSFD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311948"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1198
                     /gene="nusA"
                     /locus_tag="VFH39_01500"
     CDS             50..1198
                     /gene="nusA"
                     /locus_tag="VFH39_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6924490.1"
                     /translation="MDFKQLAQAIKLIAEEKNLPEEQVQEIVQQALAAAWRRDYGDRE
                     QEIRVTMNLNTGEVDAYVSKEVVEEVGDENYEISLGDAQVIRKNAKVGETVEIHEKVT
                     EFGRVAAQTAKQVILQRLREAEREIVMNEYEDKIGTVINATVSRVEGNIVRIDLGKAQ
                     GIMPRSEQIQGERYYPGQRLKVFLKDVERGFRGPQLILSRGNTQFIEWLFRAEVPEME
                     SGAVEIKGIAREAGVRTKIAVASNVPGVDPVGTFVGGHGTRVNAVMSETGEQEKIDII
                     VYSDNMVEYIINALSPTKVAKVTLDDKNMKAKVLVPEDQLSIAIGKSGQNVRLASKLT
                     GYDIDIEAERETADSGVVETTTLPKVPKLKKKSDLENSLLEAIEEHGE"
     gene            1262..1966
                     /locus_tag="VFH39_01505"
     CDS             1262..1966
                     /locus_tag="VFH39_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924491.1"
                     /translation="MTAAQIEAVSQEAPAQREEALWLPSDHNKLFVLRSEGVLPLYAG
                     QLAIPGIERHELADYTRRVDEANADSDSAVYVIDRPYKMRDSLGSPLTWPGVSERSLH
                     WVRVSEEPVINPDVTGGPFATLLKEGSEDAYDEAIPQPKVNYRHRAQLETKFHSLTGV
                     DDAAFTLRRRGVNTLQQRRLMAGDVRPPSETLDASEPLLSEMIAMSIIANGEAGSSRH
                     AHNLTNGVRAILRQLS"
     gene            1983..2483
                     /locus_tag="VFH39_01510"
     CDS             1983..2483
                     /locus_tag="VFH39_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924492.1"
                     /translation="MTTASELPTTSLELFPDACLQPCYGGSIGLVTPEAALTGQLALG
                     LLTRDTSVNLLERFDIATSRVALEGADARHTLHALARFSLLNSDNWAWLDGVDVKRAY
                     NDLPPISRLEARIGSDNAYRMLYGYLQHESFAPHHPGYDWLPAVHALGNNRRRISLRT
                     CAKKLK"
     gene            2633..5107
                     /locus_tag="VFH39_01515"
     CDS             2633..5107
                     /locus_tag="VFH39_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6924493.1"
                     /translation="MMPNSPDFQEFLNHLTNNALQSLKHADAIARSFGSAYVGTEHLL
                     LGVLAQDGSMGAKMLEGAGVTLERARVALNMTPKTLVINVGAKGLSETAKLTLKMAYD
                     VAQEYDQEFCGTEHILYSIISQKNARATILLRDMNINVDTLMNEVEQFLNRQQYEETE
                     SPETGRRTKKGKKTAVDVYGTDLTAEARSGKLDPVVGRDNQIRRVITILNRRTKNNPV
                     LIGEPGVGKTAIVEGLAQRIVSEDVPDSLLDKRIVLLDLAGMIAGTKYRGEFEERLKK
                     VMSELEKDSKTIIFIDELHLIVGAGAAEGAMDAGNILKPALARGKIQLIGATTTNEYT
                     KHIEKDAALERRFQPVQVPETTPAETLAILKGLRKHYESFHGVKLSDEVLDDAVAFAK
                     RYIQDRYMPDKAIDLIDETSAHLRIDKGKTSPEVRKLQKELKLVNTRIEDAVDSEDYE
                     KAAQYKQRASQINEELTKMQANDRSDKRLTVTSEDLAEVVSRMTGVPVKKVIRSEAKY
                     LLGLEKTIGKHVIGQQEAVEAVSKAIRRNRSGIGSEKRPIGSFIFLGPTGVGKTELAR
                     VLAREFFGSEKALVKIDMSEFGEHHNVSRLVGAPAGYVGYDDGGQLTDKIRRQPYSLV
                     LFDEIEKAHPDVFNMMLQILEDGHLTDAKGRRIDFTNTIVIMTSNIGADKLQKEANFG
                     FHARGNDELKELDELHAANQDKVLDELKKTMRPEFLNRIDKVIVFRALTKKDIYKIID
                     LQIDELKERLQKRGLSVQLTTAAKNHLLEHGYDAKNGVRPLRRLIQDTIEDHVAYELL
                     EDKFAKGDIVQVVTKDGELAYAVANE"
     gene            5152..6144
                     /locus_tag="VFH39_01520"
     CDS             5152..6144
                     /locus_tag="VFH39_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924494.1"
                     /translation="MQPKTKRKSKRLGALKVIGFWVVLGLVLTYNRQGIIDAWRLHTY
                     QPTPAVAAIARDDTMTPKARNIFYVNEPVITAKTAFSNQCPSGTERTVVLGCYHSNQN
                     GIFVLKVTDNRLNGVEQVTAAHEMLHAAYDRLSGSQKHTIDAELQAFYANGLTDQVVK
                     QQIADYQKSEPNDVTNEMHSLFATEVTKLPTDLETYYGRYFTDRQAVIKLYDGYEAEF
                     TGRQATIQADDTKLAAMRQQITTMEPALQQQAADLDTQRAQLEAKRQSGDVRGYNAAV
                     PGYNASVNEYNAKADTLRSLIAAYNRLVAERNAVAAETNQLTAEISNSVPSIQQ"
     gene            6198..>6933
                     /locus_tag="VFH39_01525"
     CDS             6198..>6933
                     /locus_tag="VFH39_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6924495.1"
                     /translation="MAKKPATRFVCTNCGAAYSSWAGRCAQCGEWNTLQEEAPIVVGR
                     ANAPATGKALRPESVDAVVTHDLPRLTSGIKEVDEVFGGGIVAGSVNLIAGQPGIGKS
                     TLLLQLAYAVAAHAPVLYISGEESAHQVGLRAQRLGTLRPELKLATTTSADDAARTIV
                     GGEYRLVIVDSVQTMTVEAVASAAGTVSQITNSTQLLTNAAKQSNTAVILVGHVTKEG
                     SIAGPKVLEHVVDVVLQLEGDRYGGFK"
BASE COUNT         1718 a         1962 c         2057 g         1196 t
ORIGIN      
        1 atccggccgg caggtcggat tttttcatag aaaaggagaa aatatggaaa tggattttaa
       61 acaactggcg caggcaataa agctgatagc cgaagaaaaa aacctgcccg aagagcaggt
      121 ccaggaaatc gtccagcagg ctttagccgc cgcctggcgc cgcgattacg gcgaccgcga
      181 gcaggaaatc cgcgtcacca tgaatctcaa caccggggag gtggacgcct acgtcagtaa
      241 ggaagtcgtc gaggaagtcg gcgatgagaa ctacgaaatc agccttggtg acgcccaggt
      301 catccgcaag aacgccaaag ttggcgaaac cgtggagatt cacgagaagg tgactgagtt
      361 cggtcgtgtg gccgcccaga ctgccaagca ggtgattctg caacggctgc gtgaagcgga
      421 gcgcgagatc gtcatgaacg aatacgagga caagatcggc accgtcatca acgccaccgt
      481 ttcccgcgtg gaaggcaaca tcgtgcgcat cgacctcggt aaggcgcagg gcatcatgcc
      541 ccgcagcgaa cagatccagg gtgagcgcta ctacccgggt cagcgcctga aggtgttcct
      601 gaaggacgtg gagcgcggct tccgcggtcc gcagctgatt ttgagccgcg gcaacacgca
      661 gttcatcgag tggctgttta gggccgaagt tccggaaatg gaaagcggcg ccgtcgaaat
      721 caagggcatc gcccgtgaag ccggggtgcg taccaaaatc gccgtcgcca gcaatgtgcc
      781 cggcgtcgat ccagtcggca ccttcgtggg cggtcatggt acccgcgtga acgcggtgat
      841 gagcgaaacc ggcgagcagg agaagatcga catcatcgtg tatagcgaca acatggtgga
      901 gtacatcatc aacgcgctga gcccgaccaa ggtcgccaaa gtgaccctgg acgataagaa
      961 catgaaggcc aaggtgctgg tgccggaaga ccaactgagc atcgccatcg gtaaaagcgg
     1021 gcagaacgtg cgcctggcca gcaagctgac cggttacgac atcgacatcg aagccgagcg
     1081 cgaaaccgcc gacagcggtg tggtcgagac caccaccttg ccgaaggtac cgaagctgaa
     1141 gaagaagagc gacctcgaaa actcgttgct ggaagctatc gaagagcacg gtgagtaatc
     1201 cgctagtatt gcaaaatact caaaaagtag tataatatac tgctgttaaa ggaatctcgc
     1261 catgacagca gcccaaatcg aagccgtgtc tcaggaagca ccggcgcagc gcgaagaagc
     1321 actgtggtta ccctctgacc acaacaaatt gtttgtcctt cgttcagaag gcgtgcttcc
     1381 actgtatgca ggccaattag cgattcctgg tatagagcgc catgagcttg ccgactacac
     1441 tcgtcgtgta gatgaagcaa atgccgatag cgattcagcg gtatacgtca tcgatcgccc
     1501 gtataaaatg cgggacagcc tgggtagccc ccttacttgg ccgggtgtga gtgaacgaag
     1561 cctgcattgg gtgcgggtat cagaagagcc cgtaatcaac ccagatgtaa ctgggggccc
     1621 gttcgcaaca ttgctgaagg aggggagtga agatgcttac gacgaagcta ttccgcagcc
     1681 aaaggtcaat taccgccacc gagctcagtt agagaccaaa ttccacagtc tgaccggtgt
     1741 agatgatgcg gccttcactt tacgccgccg tggcgtgaac acgctgcagc agcgccgact
     1801 gatggccgga gacgtccggc cgccgagcga gacgcttgac gctagtgagc ctctactttc
     1861 cgaaatgatt gccatgtcga taatcgccaa cggcgaagcc ggtagcagcc gacacgccca
     1921 caatctgacc aacggtgtac gagctatctt acgccagctt tcctaagcgg cttgaaccaa
     1981 gcatgactac agcatctgaa ttaccgacga ccagcctgga attgttcccg gacgcttgtc
     2041 tgcaaccgtg ctatggtggt tctatcggcc tcgtgacgcc cgaggcggcc ctgaccgggc
     2101 aattggccct tggcttgctc acgcgcgaca ccagtgtgaa cctattggag cgcttcgata
     2161 ttgctacgag ccgggtggcg ctggagggtg cagatgctcg acacacgctc cacgccttag
     2221 cgcgattttc ccttctaaac agtgacaatt gggcgtggct tgatggtgtc gatgtcaagc
     2281 gggcgtacaa tgatctacct cccatctcac gcctagaagc tcgtataggg tccgacaacg
     2341 catatcggat gctttatgga tatctgcagc acgaaagttt tgcgccgcac catccggggt
     2401 atgattggtt gccggcggtt cacgctttag gaaacaatcg tcgtcgcatt tcactccgga
     2461 cgtgcgcgaa aaaattgaaa taatcgccca gtaaggtgtc tgttagcact cttgacctta
     2521 gagtgctaac tggcgtatta taataagtag accgctactt aacaccggcg aagattacgt
     2581 aaggctgaca aatctcacag atttctcagc gggaagggcg gaaaataaac ctatgatgcc
     2641 aaattcacct gatttccagg aatttttgaa ccacctaaca aacaacgcat tgcaaagctt
     2701 gaagcatgcg gacgccattg ctcgcagctt cggcagtgcc tatgtaggca cggaacacct
     2761 gttgctcggc gtgctggcgc aggacggcag catgggagcc aaaatgctcg agggtgccgg
     2821 cgtgacgctg gagcgcgccc gggtcgcact taatatgacg cccaagacgt tggtcatcaa
     2881 cgtcggcgcc aaggggctca gcgagaccgc caagctgacg cttaagatgg cctacgacgt
     2941 cgcacaggaa tacgatcagg aattctgtgg caccgagcac atcctgtaca gcattatcag
     3001 tcagaagaac gcccgggcta ccattttgct gcgcgacatg aacataaatg tcgatacctt
     3061 gatgaacgaa gttgagcagt tcctgaaccg tcagcaatac gaagagacgg agagtcccga
     3121 gactggccgc cgtaccaaga agggcaagaa aacggcggtg gacgtgtacg gcaccgacct
     3181 gacggcggaa gcccgtagcg gcaagcttga ccccgtggtc ggccgtgaca atcagatccg
     3241 ccgcgtcatc accattctga accgccgcac caagaacaac ccggtgctca ttggcgagcc
     3301 gggtgtcggt aagacggcca tcgttgaagg cctggcccag cgcatcgtca gcgaagacgt
     3361 gccggacagc ctgcttgaca agcgcatcgt gctgctcgac ctggcaggta tgattgccgg
     3421 caccaagtac cgcggtgagt tcgaggagcg cctgaagaaa gtcatgagcg agctcgagaa
     3481 ggacagcaag accatcatct tcatcgacga gctgcacctc atcgtgggtg cgggtgcggc
     3541 cgaaggcgcc atggacgccg gtaatatcct caaaccagcg ctagccaggg gcaaaatcca
     3601 gctcatcggc gccaccacca ccaacgaata caccaagcat atcgaaaagg acgccgcgct
     3661 ggagcgccgg ttccagccgg tgcaggtacc cgaaaccacg cccgccgaga cattggcgat
     3721 attaaagggc ctgcgcaagc actacgaaag tttccatggc gtgaagctca gcgacgaggt
     3781 actggacgac gcggtggcct ttgccaagcg ctacatacag gaccggtaca tgccggacaa
     3841 agccatcgac ctcatcgacg aaacgagcgc tcacctgcgg atcgacaaag gcaagacgag
     3901 tccggaagtc cgcaagctgc agaaggaatt gaagctcgta aatacgcgca tcgaagacgc
     3961 cgtcgattcc gaagattacg agaaggccgc tcagtacaag cagcgggcca gtcagatcaa
     4021 tgaagagctg accaagatgc aggcgaacga ccgcagcgac aaacgcctga ccgtgaccag
     4081 cgaggacctg gcggaagtcg tctcgcgtat gacgggcgtg ccggtgaaga aagtcattcg
     4141 cagcgaagcc aagtacttgc tgggattgga gaagaccatc ggtaagcacg tcatagggca
     4201 gcaagaggcc gtcgaggctg tgagcaaagc catccggcgc aaccgcagtg gcatcggcag
     4261 cgaaaagcgg cccatcggca gtttcatctt cctgggcccg accggcgtcg gcaagaccga
     4321 gttggcccgc gtgttggccc gcgagttctt cggctccgag aaagcgctcg ttaagatcga
     4381 tatgagcgag ttcggcgaac atcacaatgt gtcgcgtctg gtcggtgcgc ccgccggcta
     4441 cgtcgggtac gacgatggcg gccagctgac cgacaagatc cgtcgccaac cctacagcct
     4501 ggtgcttttc gacgagatcg aaaaagcgca cccggacgtg ttcaacatga tgctgcagat
     4561 tctggaggac ggtcacctga ccgacgccaa ggggcgccgc atcgacttca cgaacaccat
     4621 cgtaatcatg acgtccaaca tcggtgccga caagttgcag aaggaagcca acttcggttt
     4681 ccacgcccgg ggcaacgacg agctgaagga actggacgag ctgcatgccg ccaaccagga
     4741 caaagtactc gatgagctca agaagaccat gcggccggaa ttcctgaacc gcatcgacaa
     4801 ggtgatcgtc ttccgcgccc tgaccaagaa agacatttac aagatcatcg acctgcagat
     4861 cgacgaactc aaggaacgcc tgcagaagcg gggtctgagc gtgcaactga cgacggctgc
     4921 caaaaaccac ttactggagc acgggtacga cgccaagaac ggcgtgcgcc ccttgcgccg
     4981 gctcatacag gacaccatcg aggaccatgt ggcctacgag ctgctggaag acaagtttgc
     5041 caagggcgac atcgttcagg tggtgaccaa ggacggtgaa ctggcttacg ctgtcgccaa
     5101 cgagtgacgt caaactagcg cagcgctggc cggcccgcta caatatgagg tatgcaaccg
     5161 aaaacaaaga ggaagtcgaa gcgactcggc gctcttaaag tcatcggctt ctgggtagtg
     5221 ctcgggctgg tgctcactta taaccgccaa ggcatcatcg atgcttggcg gttgcatacc
     5281 taccaaccga caccggccgt cgcggccatc gcccgggacg acaccatgac gccgaaggcg
     5341 cggaatatct tctatgtgaa cgagccggtc attacggcca agaccgcctt cagcaaccag
     5401 tgtccgagcg gtaccgagcg caccgtcgtg ctcggctgct accactcgaa tcagaacggc
     5461 atcttcgtcc ttaaggtgac agacaaccgt ttgaatggcg tggaacaggt gacggccgcc
     5521 cacgagatgc tgcatgccgc ctatgaccgg ctcagcggct cccagaagca cacgattgac
     5581 gccgaactgc aggcttttta cgccaacggg ctgacagacc aagtagtcaa acagcagatt
     5641 gctgactacc aaaagagtga gcccaacgac gtgaccaacg agatgcattc gctgttcgcc
     5701 acagaagtca ccaaactgcc gaccgacctc gagacgtact acggccggta tttcactgac
     5761 cgccaggctg tcatcaaact gtacgacggc tatgaggccg agttcaccgg ccggcaggcg
     5821 accatccagg cagatgatac gaaactggcg gcgatgcggc agcagatcac gaccatggag
     5881 cccgcactgc agcagcaagc ggccgacctg gacacgcagc gggcgcagct ggaggccaag
     5941 cggcaaagcg gcgacgtaag gggctataac gcggccgtac ccggctataa cgcatccgtg
     6001 aatgagtaca atgctaaggc cgatacgctg cgttctctca tcgccgccta taaccggttg
     6061 gtggcggagc gcaacgcggt agctgccgaa accaatcaat tgacggctga aatcagcaac
     6121 agcgtgccct caatacaaca atagatatct gccaacagcc gggtgtgcta gactcggtgc
     6181 taaggaagga gaaaggcatg gccaaaaaac cggcaacccg attcgtctgc accaattgcg
     6241 gtgccgccta cagttcatgg gccggccgct gtgcacagtg cggcgaatgg aacaccttgc
     6301 aggaagaagc accgatcgtg gtgggccgcg cgaatgcgcc ggcaacaggc aaagccctgc
     6361 gtccggagtc cgtcgatgcg gtagtcacgc atgacttgcc acgcttgacc agcggcatca
     6421 aggaggtaga tgaggttttc ggcggcggca tcgtggccgg cagtgtcaat ctcatcgccg
     6481 gccagccggg catcggcaag agtacgctgc tgctgcagct ggcctacgcc gtggcggcgc
     6541 acgcacccgt tttatacatc agcggtgagg aatcggcaca ccaggtagga ctgcgggcgc
     6601 agcggctcgg tacgctccgc ccggagctga agctggccac gaccacttcg gcagatgatg
     6661 ccgctcggac catcgtgggg ggagagtacc ggctggtcat cgtcgattcg gtgcagacca
     6721 tgacggtaga ggcggtagcc tcggcggccg gcacagtgtc gcagattacc aacagtacgc
     6781 aactgctgac gaacgccgcc aagcaatcga ataccgcggt cattttggtg ggccatgtga
     6841 ccaaagaggg cagcatcgcc ggcccgaaag tgctggaaca cgtcgttgat gtcgtgttgc
     6901 agctggaagg ggaccgctat ggtggattca agg
//
LOCUS       DASSFD010000069         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1414498, whole genome shotgun sequence.
ACCESSION   DASSFD010000069 DASSFD010000000
VERSION     DASSFD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414498"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1881
                     /locus_tag="VFH39_01530"
     CDS             67..1881
                     /locus_tag="VFH39_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6924496.1"
                     /translation="MAEQSQVAFDQLTEAIRSAAELLASVQDPEQLLRGVQGLGTIAT
                     GALELGHALRVGSSFEAFMGEVAASAEPQLTTSPAELLPADVEPNAKEALPIEIDATE
                     VDEDEPTPDEPVLIARDDQEVVEDAVTDDEPAATISAADEASIRSIEDHLAKRRYRWT
                     PREQRLLQALLANRGEPLAPADLMKSIDGTLAARRTALRNFGVKLRQHPAYAEAVRQS
                     GKTWQRRIWLAKDAAPGEPQAYEVRTPDQTASMVASGPESSPKSVQVTGVTVLHDTPR
                     PQSQPTTPVRVLSGVTKTAKPLRSKVTHSPPLTAPEATVASEATRNVALTSWGLTLSE
                     NGHRQLSVNGTPLRLRDASIDVLLEVCRHPQGVTFEAIRSRLAEMREYRTEPPTLSSY
                     LAEIAANLQATTDASGWSDSTIGPEDDQQRLLKIDGALTTDQDGSADFLGLPPARRPK
                     EITHWRRKTECASVDPEAFFSERSLRLVGSHCTKCVVADECLAEALQNERGLPREHRV
                     GIWGGLLPAQRQQIKGSDGIVTPAEAAYIVHVRSENAAGRTVDNSGPGRPSKEKAAAI
                     KEQQRLTPAYFQAELERAAIAVRQSVREQDDLDIAQSA"
     gene            complement(1901..>2335)
                     /locus_tag="VFH39_01535"
     CDS             complement(1901..>2335)
                     /locus_tag="VFH39_01535"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6924497.1"
                     /translation="QEYWPASAASYDDEYKRRVLGLVQERLKYFAELPSLTVFFFEDL
                     PVDMELVDGNKQLKKVDHAELKQLLEQAKASLAQSDFSTDDLTERLNRLLESTGQKPG
                     VLFSLIRVATTSAPASPGLADTLAVLGKDRSLQRIDSWLTAL"
BASE COUNT          500 a          679 c          707 g          449 t
ORIGIN      
        1 acgtagcgcc ggcttgtact taggtttcgt tgcgctataa tcctcaacaa ataacggagc
       61 ggtactatgg cagagcaatc gcaggtggcc tttgatcaac taacggaggc gattcgctcc
      121 gcggccgaat tgcttgcctc ggtgcaggat cccgaacagt tactgcgtgg cgtccagggg
      181 ttgggcacaa tcgccactgg tgctttggag ctcggccacg ccctgcgggt aggtagttct
      241 ttcgaagctt tcatgggtga agtagctgct tccgcggagc ctcaactgac cacatcaccg
      301 gctgagctat tacccgctga cgttgaaccg aacgcgaaag aggcactgcc aattgagata
      361 gacgctacag aggtggatga agacgagccg acacctgacg agcctgtcct tatcgcgagg
      421 gacgaccagg aagttgtcga ggatgccgtt acagacgatg agcctgctgc cacgattagt
      481 gcggccgatg aagccagtat ccgttcgatt gaagatcatc tggcgaagcg gcgctaccgc
      541 tggacgcctc gagagcagcg actgctgcaa gcgctgctgg caaatcgcgg cgagccatta
      601 gctcccgctg atctcatgaa gagtattgac ggcactctgg ctgctcgtcg cactgctctt
      661 aggaatttcg gcgtaaagct caggcagcac ccggcgtatg ccgaggctgt ccggcaatcc
      721 ggcaagacct ggcagcgacg aatctggctc gctaaggacg ctgcccctgg tgagcctcaa
      781 gcctacgagg tacgtactcc cgatcaaacg gccagcatgg tcgcctcggg cccggaaagc
      841 agccctaaaa gtgtacaggt aactggcgta acggtcctgc atgatacccc gcgcccgcaa
      901 tcgcaaccta ccacacctgt ccgggtgctt tcaggcgtaa ccaagacagc gaagccactt
      961 cgaagcaaag taacgcacag ccccccgctt accgccccgg aagcgaccgt agcatctgaa
     1021 gccacccgca acgtggcgct tactagctgg gggctcaccc tcagcgagaa tggccaccgg
     1081 caattgagcg tcaatggtac gccgcttcgc ctacgggatg ccagtatcga tgtgctgctg
     1141 gaagtctgcc gtcatccgca aggtgtgacc ttcgaagcta tcagaagtcg cctggccgag
     1201 atgcgtgagt accggactga accgccgacc ctcagcagct atctcgcaga aatcgccgct
     1261 aacctgcagg cgaccaccga tgccagtggc tggtcagaca gcaccatcgg ccccgaggat
     1321 gatcagcaac ggcttttaaa aatcgacggc gcattaacta ccgaccagga tggttcggct
     1381 gattttttag gcctaccgcc agcaaggagg ccgaaggaaa taacgcactg gcggcggaaa
     1441 acggaatgtg cgtcggttga ccccgaagca tttttttctg agcgctcatt aaggctggtc
     1501 ggcagtcatt gtacgaaatg cgtcgtggca gacgaatgct tggctgaggc gctccaaaac
     1561 gaacgcgggc tgccgcgtga acaccgggtg ggtatatggg gcggtctgct ccctgcccag
     1621 cgacagcaaa tcaaagggag cgacggcatc gttacgccgg ccgaagccgc ttacatcgtg
     1681 catgtccgca gcgagaatgc cgcaggacgg acagttgata attccggtcc cggccgtcct
     1741 tccaaagaga aggcagcggc gataaaggaa cagcagcgtc tgacacccgc ctattttcag
     1801 gctgaactgg agcgagctgc aattgccgtc aggcaatcag tacgcgagca agacgacctg
     1861 gacatagccc aatcggctta gtgggtacgg gcggcttagc ctacaatgcc gtcagccagc
     1921 tgtcgatgcg ctgcagactg cggtctttgc ctaggaccgc gagggtgtcg gccaggccgg
     1981 ggctggccgg tgcgctggtg gtggccaccc ggataaggct gaacaggacg cctggcttct
     2041 gaccggtgct ttcgagcaat cggttgagcc gctccgtgag gtcgtcggtg ctgaagtcgc
     2101 tctgcgccag gctggccttg gcctgctcca gcagctgttt cagctccgca tggtcgactt
     2161 tcttgagctg cttgttaccg tctaccagct ccatatcgac cggcagatcc tcgaagaaaa
     2221 agacggtgag gcttggcagt tcggcgaagt acttgaggcg ttcctggacc aaaccgagga
     2281 cgcggcgttt gtattcatcg tcgtagctgg cggcgctggc cggccagtac tcttg
//
LOCUS       DASSFD010000070         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_212709, whole genome shotgun sequence.
ACCESSION   DASSFD010000070 DASSFD010000000
VERSION     DASSFD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212709"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFH39_01540"
     CDS             <1..1197
                     /locus_tag="VFH39_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6924498.1"
                     /translation="VDWRSAFRRLRASTAALALLLGIAGGFLGGWVAGQNGDGSGGVL
                     NGSLSSQEKIVTSESNLINSIAKSVGPSVVSVNVTSTTNTPSFFGFVQPTQQQAAGTG
                     IIINSDGLVLTNRHVVPDGTTSVSVTLSDGTELKNVQVLGRTSESSSLDIAFLKIRDT
                     KGHQLQPAVLGDSSKVQVGDAVVAIGNALGQFQNTVTSGIISGYGRSVQAGDSSGGNS
                     ENLDDLFQTDAAINEGNSGGPLTNLNGQVIGVNVAIAGNAQNIGFAIPINDVKGLINQ
                     VVKTGKLQQPYLGVRYIPLTADIAQQYGLSVENGAFIAPAADATNPSVVPGSPADSAG
                     LQEGDIITKVDGTDIDQNHSLTGLLGRHQPGDKVTLSVVRDGHTKQIDVTLGTMPSAS
                     DQSPGQ"
     gene            complement(1201..2025)
                     /locus_tag="VFH39_01545"
     CDS             complement(1201..2025)
                     /locus_tag="VFH39_01545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HET6924499.1"
                     /translation="MGTTVGEWLLSANALLTRVGIGTAHLDAVVLLEDCIKHDRAWIL
                     AHTEHPLSEEHLARLNEQLNRRAQHEPLSYIRNKTEFYGREFYIDHRVLEPRPESETM
                     IDMLKSLHLPREITVIDVGTGSGALAITARLEVPRADVMATDLDPEALKVAEQNCEKH
                     SCDIDFLKGNLLKPFYLHYHPRHSVVLANLPYVPDSFHINPAAMREPRAAIFGGPDGL
                     DLYRELFEQVDPLPYKPLYILTEAMPPQHELLRSIASRHHYRQSAYDDFIQVFRPA"
     gene            complement(2469..>3011)
                     /locus_tag="VFH39_01550"
     CDS             complement(2469..>3011)
                     /locus_tag="VFH39_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HET6924500.1"
                     /translation="KFEAGVHRVQRVPVTESQGRIHTSTTTVAVLPEAEETDVEINPN
                     DLRIDVFRSGGHGGQSVNTTDSAVRITHLPTGIVVTNQDEKSQIKNKAKALGILRSRL
                     LQMKIEEDQKQLDDKRRALIGSGDRSEKIRTYNFPQDRITDHRIGYSRSNIPGALNGE
                     IDDLVEHLQAAEHELISNER"
BASE COUNT          596 a          855 c          898 g          662 t
ORIGIN      
        1 gttgactggc gttcggcatt caggcgcctg cgtgccagta cggcggcgtt ggcgctgttg
       61 ctcgggattg ccggcggctt cctgggcggc tgggtggcgg gccaaaatgg tgatggcagc
      121 ggcggcgtgc tgaacggctc cctgagcagc caggaaaaga tagttacctc cgagagtaac
      181 ctcataaatt ccattgctaa atcggtcggt ccgagcgtgg tcagcgtcaa cgttaccagt
      241 accacgaaca cgccaagctt tttcggcttc gtgcagccga cccagcagca agccgccggc
      301 acgggcatta ttatcaatag cgatggcttg gtgcttacca atcgccacgt tgtacctgac
      361 ggcacaacca gcgtgagtgt taccttgagc gacggcaccg agctcaagaa cgtgcaggtc
      421 ctgggccgta ccagcgagag cagcagtctg gacatcgcct ttttgaaaat ccgcgacacc
      481 aaggggcacc aactgcaacc ggccgtgctg ggagactcct cgaaagtcca ggtcggcgat
      541 gccgttgtgg ccatcggcaa cgctctcggc cagttccaga acaccgtcac cagcggcatc
      601 atcagcggct atggccgcag tgtccaggcc ggtgacagca gtggcggcaa cagcgagaac
      661 ctggacgatc tgttccagac agacgctgcc atcaacgaag gcaacagcgg cggtccgctc
      721 accaacctga acggccaagt cataggcgtc aacgtggcca ttgccggcaa tgcccagaac
      781 atcgggtttg ccatacccat taacgacgtc aagggcctga taaaccaagt tgttaagacc
      841 ggcaaacttc agcagcctta cctgggcgtg cgctatatac ctctgaccgc cgacatagcc
      901 caacagtatg gccttagcgt ggaaaacggc gccttcatag cgcccgccgc cgacgcgacg
      961 aatccgtcgg tcgttccggg cagtccggct gacagcgccg gtctgcaaga aggtgacatc
     1021 atcaccaaag tagacggcac tgacatagat cagaaccaca gcctgaccgg cttgctgggc
     1081 cgtcaccagc ccggcgacaa agtcaccctc tccgtggtgc gggacggcca cacgaagcaa
     1141 atcgacgtga cgcttggcac gatgcccagc gcatccgatc agagcccggg ccagtaactt
     1201 ttaggccggc cggaagacct ggatgaaatc gtcgtaggcg gactgccggt agtggtggcg
     1261 gcttgcgatg cttctcagca attcatgctg cggcggcatg gcctcggtca gaatgtacag
     1321 cggtttgtag ggcagaggat cgacctgctc gaacaactcc cggtacagat ccagcccgtc
     1381 cgggccgccg aagatggccg cgcggggttc gcgcatggcg gcgggattga tatggaagct
     1441 gtcgggcacg tagggcaggt tggcgagcac caccgaatgc ctgggatggt aatgcaggta
     1501 gaacggtttc agcagattgc ccttcagaaa gtctatgtcg cagctgtgtt tttcgcagtt
     1561 ctgctcggcc actttgaggg cttcagggtc caggtcggtc gccataacat cggcgcgcgg
     1621 cacttcgagc cgggcagtga tggccagggc gccgctgccg gtgccgacat ctataacggt
     1681 tatctcgcgg ggcaggtgca gacttttaag catgtcgatc atcgtttcgc tctccggccg
     1741 cggttcgagc acccgatggt ctatgtaaaa ttcgcggcca taaaactcgg tcttgttgcg
     1801 gatgtaactg agcggttcat gctgcgcccg gcggttcagc tgttcgttca gccgtgccag
     1861 atgttcttcg ctcagagggt gctcggtatg cgctagtatc caggcccggt catgttttat
     1921 gcagtcttca agcaacacca cggcgtccag atgcgccgtg ccaataccga ccctggtaag
     1981 cagggcgttc gcactcagca gccactcgcc gacggtcgtt cccatagtgc atatattatg
     2041 cactaccagc gcgaattgtg cagtaataat gattaatgac taatgaataa gtccggccag
     2101 ggagatttgg gttgttttcc gagagtctga gccttgaccc gtagtaattt agaaacttca
     2161 acgccaagcg catatgccgg cttaatttca ttgtcttttt gtctttgagg cagctttcat
     2221 cctttgcaaa gatgaggata aaccgtgatt cccggagcga accgtaggat atcttcagaa
     2281 accgtgcctg ggttttggcg gtttgacggg cgaaaccctc accatatcac ggggtaccga
     2341 taatgcggca cgccgcaacc gtggacctgg cccggacatt tcttttttgg tagctggcgc
     2401 gccaagccgg tgcacaatgg gcataatccc tggctttgtc cagtaatacc ccgggagtta
     2461 ttcttcaatc accgttcgtt acttatcagc tcgtgctcgg cggcttggag gtgctctacc
     2521 aggtcgtcaa tttcgccgtt cagggcgccg ggaatattgc tgcggctgta gccgatgcgg
     2581 tggtcggtta tacggtcctg ggggaagttg taggtgcgga tcttttccga cctatccccg
     2641 gaacctatca aggcgcggcg cttgtcgtcc agctgtttct gatcttcttc tattttcatc
     2701 tgcagcagcc tggagcgcag tatgcccagg gctttggctt tgttttttat ctggcttttc
     2761 tcgtcctgat tagtcaccac gatgccggtg ggcaagtggg tgatgcgcac tgcgctgtca
     2821 gtggtgttta cggactggcc gccgtggccg ccgctgcgga acacgtctat gcgcaggtcg
     2881 ttcggattta tttccacatc ggtctcctcg gcttccggca gtaccgcgac ggtagtcgta
     2941 gaagtgtgga tgcggccctg gctttcggtg acgggcaccc gctgcacgcg gtgtacgccg
     3001 gcctcgaact t
//
LOCUS       DASSFD010000071         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1661643, whole genome shotgun sequence.
ACCESSION   DASSFD010000071 DASSFD010000000
VERSION     DASSFD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661643"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFH39_01555"
     CDS             complement(<1..480)
                     /locus_tag="VFH39_01555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6924501.1"
                     /translation="MYQRAIPYMPPIATSTVSMTMSTRRCHQRRGHHRMAGIGRLASL
                     LRDGALCLLMWFSASRCYNGSSEMSVSVKALTISLVIPVYNEEDHLDACLRSVMAQRI
                     PFDEIVVVDNNSTDKTLWIARTFPNVRIVRETRQGVVHARSTGFDHARGDIIARIDAD
                     "
     gene            425..1417
                     /locus_tag="VFH39_01560"
     CDS             425..1417
                     /locus_tag="VFH39_01560"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET6924502.1"
                     /translation="MLTVLVAIGGMYGIARWYIHQEGDRPATYGVSFIPDYASYLGLD
                     PQKTMDALISDLHVRQFRLTSYWSDIETSPGQYDFSTLDWEFTKADAAHAKVNLTVGL
                     RQPRWPECHAPTFYDTSQPESTWYPQLKAFMTAVVNRYKGNPALAGYQIENEYFLQGF
                     GTCTNRDRQRLVDETALVRRLDPRQTIIIGRSNNALGWPIGAPQPDEFSISVYRRVWD
                     ANVSHRYLEYPFPAWFYAFTAGWQKIMTGHDMIIGELQAEAWPPNKQTIPDTSLNEQS
                     KSIDAQRLKTTIQFGRATGMKTIDLWGAEYWYYRKTVLHDPSLWDTARPAFTTH"
     gene            1441..2274
                     /locus_tag="VFH39_01565"
     CDS             1441..2274
                     /locus_tag="VFH39_01565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6924503.1"
                     /translation="MSRNVDVVAAIPNYNMADSLSVLLPQVLEQDYAAVYVMDDKSTD
                     HSMDVLQSFAPDVTVIAGRENAGSSANRNRLIPVLGNNCIVHFLDADVRLVSDENPAR
                     LREHMQSRIGMLGGLVVDTTGQQSPWNYGPRFSLHMSVSAMLQFKLYGRHPRGLRHFM
                     RDWPETSQIPVARNTFWVVESNMAMPSGVFKHHGFDPKLREHDIQDLAIRLQRLGHIS
                     RFVPDVAVEHTAIDVRDSNRQLAMRRAEMYIARKEGVFDWLLPDGYFRPRHHGDDETG
                     Y"
     gene            2258..>2838
                     /locus_tag="VFH39_01570"
     CDS             2258..>2838
                     /locus_tag="VFH39_01570"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET6924504.1"
                     /translation="MKPVIDPERFGISFSLKQCRNFGVDQLKALDWLLAQGWRRFRLT
                     SYWDEHEPEQGTYDFKALDAQIRRITEAGGVFTLCLGARQPRWPENHWPNWAWLTTKG
                     KRTEALLRYLEVVVDRYRDEPALIGYQLENEALLKSFGKRAEVDRPRLRAEFALIKRL
                     DPVRPVIMSTSTSWGIPLRQPIPDIVGFSYYHV"
BASE COUNT          602 a          864 c          807 g          565 t
ORIGIN      
        1 gtcggcgtct atccgggcga ttatgtcacc gcgggcatga tcgaaaccgg tgctgcgggc
       61 atgcaccacg ccctggcgcg tttcgcgcac gatgcggaca ttggggaacg tccgggcgat
      121 ccacagggtc ttgtcggtgg aattgttatc caccaccacg atctcatcaa aaggaattcg
      181 ctgagccatc actgagcgca aacatgcgtc cagatggtct tcttcgttat agactggtat
      241 aactaatgat atcgttaacg ctttaactga cacagacatt tccgatgaac cattgtagca
      301 ccgcgaagct gagaaccaca tgagcaagca taacgcgccg tcgcgtaaca gagaggctag
      361 ccgtccaata ccagccatcc gatggtggcc gcgccgctgg tggcaccgcc tagtgctcat
      421 cgtcatgctt accgtgctag tagcaatcgg cggcatgtac ggtatcgccc gctggtacat
      481 tcaccaggaa ggcgacagac ccgctaccta cggcgttagt ttcattccgg attatgccag
      541 ttacctcggg ctcgaccccc aaaagacaat ggatgcgctc atatccgacc tgcacgtgcg
      601 gcagttccgt ctgaccagtt actggagcga catcgagacc agccccgggc agtacgactt
      661 cagcacgctc gattgggaat tcacgaaggc cgatgccgcc catgccaaag tgaacctgac
      721 ggtcggcctg cgccaaccgc gctggccgga gtgccacgcg ccgacgttct acgacacctc
      781 gcagcccgaa agcacctggt acccgcaact caaagccttc atgacggccg tggtcaaccg
      841 atacaagggc aatccggcct tagccggcta ccagatagag aacgagtact tcctgcaggg
      901 cttcggcacc tgcaccaacc gcgatcgtca gcggttggtg gacgagaccg ccttggttcg
      961 ccgccttgat ccgcgccaga ccatcatcat cggccgcagc aacaacgcgc tcggttggcc
     1021 gatcggggca ccgcagccgg acgagttcag catatcggtg taccgccggg tgtgggacgc
     1081 caatgtcagc caccgttacc tggagtaccc gttcccggcc tggttttacg cctttacggc
     1141 cgggtggcag aagatcatga ccggccacga tatgatcatc ggcgaattgc aggccgaagc
     1201 ctggcctccc aacaagcaga cgattccgga cacgagcctg aacgagcaga gcaagtccat
     1261 cgatgcccag cggctcaaaa cgaccatcca gttcgggcga gccactggca tgaaaactat
     1321 cgacctgtgg ggcgccgaat actggtacta tcgcaaaacc gtccttcatg acccctccct
     1381 gtgggacact gctcggccgg cattcacgac gcactgatta tttgctacaa ttagcagatt
     1441 atgtcgagaa atgttgacgt cgtcgcggcc attccgaact acaatatggc tgatagcctg
     1501 agcgtcctgc tgccgcaggt gctggagcaa gattatgctg ccgtctatgt catggatgac
     1561 aaatctaccg atcattcgat ggacgtcctg caaagctttg ctccggatgt tacggtcata
     1621 gccggtcgtg aaaacgccgg ttcctcggcc aaccgcaacc ggctcatccc ggtactcggc
     1681 aataactgca tagtgcactt tctggacgca gatgtccgac tggtgtcaga cgagaacccg
     1741 gcacgtctgc gcgagcatat gcaatctcga attggcatgc tgggcggcct ggtcgtcgat
     1801 actaccggtc agcagtcgcc ctggaattat ggaccgcgct ttagcctgca catgtctgta
     1861 tcggctatgc tccagttcaa actgtacggt cgtcacccgc gagggctgcg acattttatg
     1921 cgtgactggc cggagacgtc gcagattccg gtagctcgta atacattctg ggtggtcgag
     1981 tcgaacatgg ccatgccgag cggcgtcttc aagcatcatg gtttcgaccc aaagcttcgc
     2041 gagcatgaca tccaggacct ggcgattcgc ttacaaaggc ttgggcacat aagtcgtttc
     2101 gtgcctgacg tcgccgttga gcacaccgct atcgatgtcc gtgactccaa ccgccagctg
     2161 gcgatgcgcc gcgccgagat gtacatcgcc cgcaaagaag gtgtttttga ctggcttctg
     2221 cccgacggct atttccgacc ccgccatcat ggagacgatg aaaccggtta ttgatcccga
     2281 gcggttcggt atcagctttt cgctgaagca gtgccggaac ttcggtgtcg accagctgaa
     2341 ggcgctggat tggttgctag cgcaaggctg gcgccgcttc cggctgacca gctactggga
     2401 cgagcacgag ccggagcagg ggacatatga cttcaaggct ctggacgcac agatacgacg
     2461 catcaccgaa gccggcggcg tctttacgct gtgcttgggc gcccggcagc cgcgctggcc
     2521 ggagaatcac tggcccaact gggcctggct gaccaccaaa ggcaagcgca ccgaagcctt
     2581 gctgcggtat ctggaagtgg tcgtcgaccg ctaccgggac gagccggcac tcatcggcta
     2641 ccagttggag aacgaagcct tgctgaaaag cttcggcaaa cgggcggaag ttgaccggcc
     2701 gcggttgcgc gccgagttcg cgctcataaa gcggctggac ccggttcggc cggtcatcat
     2761 gagcaccagt acctcgtggg gcatcccgct gcggcaacct atccccgaca tcgtcggctt
     2821 ctcgtactac cacgtcta
//
LOCUS       DASSFD010000072         9026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1088993, whole genome shotgun sequence.
ACCESSION   DASSFD010000072 DASSFD010000000
VERSION     DASSFD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088993"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFH39_01575"
     CDS             complement(<1..661)
                     /locus_tag="VFH39_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924505.1"
                     /translation="MRLKLFLASIITALLTASLSSVALAGGPDATNLDLTPSAATAIA
                     DNSATITFSLFSYEYLCSGSSQTNVTSHDASGCTAQGYPNATKEAVCYNVPEDISVSG
                     SGNTLSATSVIPDPNGTCKASFTLRSSAAGTKTVHVQNEYWPTQEFTDATVTFTAPPV
                     KPAPAPSAPAAHTTTTAPSTPKPPASPSLDTLKVGNEQQTENAADTSSTTTVKQGQPL
                     VL"
     gene            917..1984
                     /locus_tag="VFH39_01580"
     CDS             917..1984
                     /locus_tag="VFH39_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924506.1"
                     /translation="MLLFGGMAYAATANPVVQGTHNSSIYKTGQTVTINGAINGDVFC
                     AGQTVNVHATVHGDILCAGQTVNVDGQVFGSVRLAGQTVNLTAQVTHGASLVGQDLTV
                     QSGARIGQDVSLAGQTVTVNGSVGRDISAASSNLTLASAVGRNVTAQVEQLTLNGGAK
                     IGGSLTYTSPNVAQLQSGAVVSGKTTHNVTAVHHANRHAFLFFRLYWLVALIVFGIVL
                     AAIFGRLFRRINPAWGTDFWIALLTGFVASIVVPIGILILFATVIGVPLALLVLLLWL
                     VAALLSLPVSGYFTGSLLLRRSHPVLIMLVGTVILALLTLIPFVGWFIALIIYWLGVG
                     IILVGIRRHYPRKIPVRSEGA"
     gene            1988..2185
                     /locus_tag="VFH39_01585"
     CDS             1988..2185
                     /locus_tag="VFH39_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924507.1"
                     /translation="MADKPKKTVVQRGGAGGIWFMGAIGTLVYFLHFHSGTFWLVILA
                     IIKAILWPGFLLYYVLRFMHI"
     gene            2305..3522
                     /locus_tag="VFH39_01590"
     CDS             2305..3522
                     /locus_tag="VFH39_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6924508.1"
                     /translation="MKHPDWKLLYGLGLPSLGITFGVTVLSAFLPTVLHGLANPIVVG
                     LVIGAEGLFGLFMPIIMGALVDRVKKVHDRLRYLVPATIAMAVALALMGVFDNLAFIA
                     VMVGLFYVGYYAYLAPYWALYPDLIPKKFSGRSRSAESVWRVMGSLTALVSGGFLITL
                     WQPLPFIVGACLVVGATMWLGWYVGTLPDKAVRHQRKRLSESFRSQWDLLYHNAPIRN
                     LAIANALWNAALRSILAFVVLFFTIGLGRSHGFVSGFVFPVAAIGIGIMAPLSGKLAD
                     RYGHVRVLAIASLVYGIGDLLPGITQESWVFACVPLVAAAAATVMTLPYAALMRLLNG
                     ETHGEVSGLFGFSRGVGSFAGPLLAGLAVELGRGFFASTQGYAAFWLATGAYVLISLF
                     FLWRLDVTSPRSV"
     gene            3584..3657
                     /locus_tag="VFH39_01595"
     tRNA            3584..3657
                     /locus_tag="VFH39_01595"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3616..3618,aa:Cys,seq:gca)
     gene            3895..3982
                     /locus_tag="VFH39_01600"
     tRNA            3895..3982
                     /locus_tag="VFH39_01600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3929..3931,aa:Leu,seq:taa)
     gene            4144..4219
                     /locus_tag="VFH39_01605"
     tRNA            4144..4219
                     /locus_tag="VFH39_01605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4177..4179,aa:Ala,seq:cgc)
     gene            4320..5954
                     /locus_tag="VFH39_01610"
     CDS             4320..5954
                     /locus_tag="VFH39_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6924509.1"
                     /translation="MIRGFLSLYRPRYARVIVRLLQDNQYRAWDYLKAYWHTADFSRA
                     SYLHDFQDTAANRILRRAVAVGMIVQLLVAAWVAVLWHWEGLSGGSLFALALLVSYPV
                     VWAHVLALVAGVRNAIRLLAHPKKLGRSIVCRILEGQVRKLLRRNEVKIVAVAGSVGK
                     TSTKLAVAKLLECQFRVRFQNGNYNDRVTVPLVFFGRREPNILNIFAWLKIFVANARA
                     LSRPYPYDVVVVELGTDHPGSMIEFGYLPVDIAVVTAVTPEHMEYFKTLDAVAAEELT
                     VFDFADTVLVNGDAVPAEYLVGRKFREYSLRSDQANYFAAVTDPSLAGQKLRLRLANG
                     HSLSMVSRYVGEPGASIVLAAASVADQLDAEHSEIAKGIANLEPFAGRMQLLNGQKDS
                     VLIDDTYNASPVAVEAALDVLYAQPAKQRIAILGSMNELGDYSPAAHKEVGKYCDPKK
                     LDLVVTIGHDAKKYLAPAAKKAGCTVHTFLSPYEAGDFVLSKLRKGALVLAEGSQNGV
                     FAEESLKPLLADPADADKLVRQSKYWLKKKQQQFRR"
     gene            complement(5944..6843)
                     /locus_tag="VFH39_01615"
     CDS             complement(5944..6843)
                     /locus_tag="VFH39_01615"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET6924510.1"
                     /translation="MTKVHVLCGGTSSEREVSLRSGNAVAVALSRAGFVVETFDLADA
                     DMAAIIACDVVFPVLHGKGGEDGVLQRQLEASSAKFVGSGSSASALCFDKWAYRQKGV
                     PAGLPMAEATLVKVNTYRTHPLAAKPFVLKPVDGGSSIDTHIVRDVTQVPQTAIADSF
                     SRHPELLMEALVEGSELTVGVLGNEALPVIEIIPPATGEFDYENKYNGASQELCPPRH
                     IDTEVQARAQELALQAHSLTGCRHFSRTDIMYDRASDRLYILETNTIPGMTAQSLFPK
                     MAASAGLDMPSLCRRLVEMALSV"
     gene            6917..8389
                     /locus_tag="VFH39_01620"
     CDS             6917..8389
                     /locus_tag="VFH39_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924511.1"
                     /translation="MHSLETIPVYESDRSPTGDQLKSRYDIYADRIEIYDIVWGDESF
                     DSPLFVELANHDALRRLQTVEQLTLPDRYKTIPGSTYFSRWEHAWGSAIFAKRLAQEM
                     GYGQREVEEIAARVLLSDVCHAAHSHALDWIAQGAGQLETLHDERRESYAEAVGINEI
                     LRRHGFDPQRILREEKDGIFDAVMPELDVDRVDYTLRESYRWVAQIPEYRSLLNKDSF
                     TVKNGRLVCRNKTAAKLFGISYTLLATEHWQEPAHRLQLELFMESMKRIFVARDGRRE
                     DVGTYSPLDYLMVTDDTLQRLANDHDEYLPMMHELMRGVSESESNSRWRARAERARAA
                     LTTGLGGASSSIEWITAHYDTLPRSYEIYPAHESRLRNGRYATLIMLDKLRKRSVDPL
                     YIDEAAEVCRLSETDEAYREYEARAIGNVQQDWRAGIIGNQASTMALKACLKENRAVW
                     PGIMARSRMPNSVLRELLRQTVRTSNGAAAKAIDCRIHSA"
     gene            8435..>9026
                     /locus_tag="VFH39_01625"
     CDS             8435..>9026
                     /locus_tag="VFH39_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6924512.1"
                     /translation="MKRYNPKDIEPRWQQYWDEQQTYKADLNSDKPKFMSMSMFNYPS
                     GAGIHIGHAMNYTISDVLARFKRQQGYESYHPVGWDAFGLPAENYAIKSGVSPQASMA
                     KIIPMYHKQYKAMGWSNDWSKEIATHEPSYYKWTQWIFSSMYRHGLVYQDARMQWWCP
                     IDNTVLANEQVIDGKCWRHDGPNDPPAEKREVKQWFF"
BASE COUNT         1874 a         2472 c         2687 g         1993 t
ORIGIN      
        1 aaagcaccaa cggctggcct tgtttgacgg ttgtagtgga cgaagtgtcg gcggcgtttt
       61 ccgtctgttg ttcattgccg actttaaggg tatcgagtga cgggctggcc ggcggcttgg
      121 gggtcgatgg tgcggttgtc gtggtgtgcg cggctggcgc acttggcgca ggagctggct
      181 tgaccggcgg ggcggtgaag gtcacggtcg cgtcggtgaa ctcctgggtg ggccagtatt
      241 cattctgaac gtggacggtc ttcgtgcctg ccgctgagga tcgaagggta aagctggcct
      301 tgcaggtgcc gttcggatcg ggtatgacag acgtagccga taaagtgtta ccgctgccgg
      361 atacgctaat gtcctcggga acgttatagc acactgcttc ttttgtggcg ttggggtatc
      421 cctgggcggt gcagccactt gcatcgtgag atgtcacatt ggtctgcgaa gaaccgctgc
      481 agagatactc ataagaaaaa agcgagaaag taatggtcgc actgttatcg gctatggcgg
      541 tggcggctga tggtgtcagg tcaagattgg ttgcatccgg accgccagcc agcgctacgc
      601 tggatagtga agcggtcaga agagcggtaa tgatggaagc gaggaaaagc ttcagtctca
      661 tagtatttct tttttgtgtt atttttgtga gcgtgaagcc atttaacccc tgcatgacac
      721 atatgtcaaa caacggcaag cggaacggca atctaacaga ggtgcaagcg gctcggtagc
      781 ggcaaacttg tgacgccgct catagcgatg agcgcgcctc ggtgagacac tgcaggtgac
      841 gggtagcgac agagggagtg agggcatgca cggactgaaa cgactttggg cctcggtggt
      901 aacgaccata gcgacagtgc ttctgttcgg tggcatggca tatgcggcca ccgccaaccc
      961 ggtggtgcag ggcacgcaca atagctcgat ttacaagaca gggcagacgg tgacgataaa
     1021 cggtgccatc aacggcgacg ttttctgtgc cggccagacg gtgaacgtgc acgctacggt
     1081 gcacggtgac atattgtgtg ccggccagac ggtgaacgtc gacggtcagg tattcggcag
     1141 tgtacggctg gccgggcaga cggtgaatct gacagcacag gtgacacatg gcgcctcgct
     1201 ggtcggccag gaccttaccg tgcaaagcgg ggcccgcatc ggtcaggatg tcagcctggc
     1261 cgggcagacg gtgaccgtga acggctcggt cggccgcgat ataagcgctg ccagcagcaa
     1321 tctgacgctc gctagcgcgg tcggccgtaa cgtgacggcc caagttgaac agctgaccct
     1381 gaatggcggt gccaaaatag gcggcagctt gacctatacc agtccgaatg tcgcacagct
     1441 gcagagcggg gcggtggtga gcggcaaaac gacgcataat gtcacggccg tgcaccacgc
     1501 caatcggcac gccttcctgt tcttccgctt gtactggctg gtcgccctga tagtcttcgg
     1561 catcgtgctg gcggccatct tcggccgcct gttcaggcgc atcaacccgg catggggcac
     1621 ggacttctgg attgccctgc tcactggttt cgtggcatcc atcgtggtgc cgataggcat
     1681 actcatactc ttcgccaccg tcatcggtgt gccgcttgcg ttgctcgtat tgctgttgtg
     1741 gctggtggcg gccttgctca gtctgccggt cagtggctac ttcaccggca gcttgttgtt
     1801 gcggcgttcg cacccggtgc tgatcatgct ggtcggtact gtaatcctgg ccttgctcac
     1861 gctcataccg ttcgtcggtt ggttcatagc ccttatcatc tactggctcg gcgtcggcat
     1921 aatactcgtc ggtatacggc gacattaccc gcggaagata cctgtacgct cggagggcgc
     1981 ataagctatg gcggacaaac cgaagaaaac agtggtgcag cggggcggcg ccggcggtat
     2041 ctggttcatg ggtgccatcg gcacgctggt gtactttctg cactttcatt ccggcacttt
     2101 ctggctggtg attctggcta tcatcaaggc tatcctgtgg cccggcttcc tgctgtacta
     2161 cgtcttacgc ttcatgcata tctgatagcg tagtcaaaga gtgaaatttt cgtcacggtg
     2221 aaaacgcggc gctggctata ctgtgggcag acgtgctatg acgacagatg acactactga
     2281 gaaatgaaga ggttacggca cggtatgaaa catcccgatt ggaagttgct ctacggtctc
     2341 ggtctgccca gtttaggaat tacattcggc gtcacggtgc tgtcggcgtt cttgccaact
     2401 gtattgcatg gcctggcgaa tccaatagtc gtcgggttgg ttattggggc cgaagggctg
     2461 ttcggtctgt tcatgccgat catcatgggc gcgctggtcg accgggtcaa aaaagtccat
     2521 gaccgcttgc ggtaccttgt gccggcgact atcgccatgg ccgtggcgct ggcgctgatg
     2581 ggagtgttcg acaacttggc tttcatagcg gtaatggtcg ggctgttcta tgtcggctac
     2641 tatgcgtatc tggcgcccta ctgggccttg tatccggacc tgattcctaa aaaattcagt
     2701 ggtcgctcgc gtagtgcgga aagtgtctgg cgggtcatgg gttcactgac ggctctggtg
     2761 agcggcggct ttttgattac tctttggcaa ccgctgccgt tcatagtcgg cgcttgtttg
     2821 gtcgtggggg ctaccatgtg gcttggttgg tacgtgggca cgctgcccga taaagccgtc
     2881 cggcaccagc gtaagcgctt gtcggaatcg ttccgcagcc agtgggacct gttgtaccac
     2941 aatgcgccga tccgcaatct ggcgattgcc aatgcgttgt ggaacgcggc gctgcggtca
     3001 atcttggcat tcgtagtgct gttctttacc atcggcctcg gccgcagcca cggcttcgtg
     3061 tccggctttg tctttccggt ggcggcgatc ggcatcggca ttatggcgcc gctgagcggc
     3121 aaactggccg accggtacgg tcacgtacgg gtactggcta tcgccagctt ggtgtatggc
     3181 atcggcgact tactgccggg aatcacccag gaatcatggg tcttcgcctg cgtgccgttg
     3241 gtggccgcgg ctgccgcaac tgtgatgacg ctgccgtacg ccgcccttat gcgcttgctg
     3301 aacggcgaga cgcacgggga ggtttccggc ctcttcggct tcagccgagg cgtgggatcc
     3361 tttgccggac cgctgctggc gggactggct gttgagctgg gccgggggtt ctttgcctcc
     3421 acccaggggt atgccgcttt ctggttggcc accggtgctt atgtgctcat cagtctgttt
     3481 ttcttgtggc ggctggatgt gacgagcccc cgatctgtct gatggcccca ggtccgtgaa
     3541 acctcaggct gccatcctgt ataatggtcc agcgtaccag cacggcgcct tggcggagta
     3601 gctacgcagc ggtctgcaaa accgcgtaca cgggtgcaaa tcccgtaggt gcctccagct
     3661 cgttcgatag ctccagcttc catgaaaaag ctggagcttt ttcattttca acttccgtat
     3721 cggagcctcg cttgaccccg gccagaatcc tctctggccg gggagtttcg ccgtcattcc
     3781 gtacaggctc gattatccgc gaggccgccg ctcgaccacg ggctttttga attaccaatt
     3841 cgtgtctccg cgaaacagcc gacgtctctg ttataatgca ggaagctcac tcgggggcga
     3901 gtggcggaat tggtatacgc ggcggactta aaatccgctg gtcttaacaa ccttgtgggt
     3961 tcaagtccca cctcgcccac cacactaaaa tatcccgttt aagacgggat attttagtgt
     4021 ggtgccggca taggctggga cttgaacggg actgccggtg gcagtcccgg cgacagacca
     4081 acctaccgga atacgagcac ttccaacgcc aggttgttcg tgctattgtt acacctgtat
     4141 ttcggggacg tagctcacct ggtagagcgc agcattcgca ttgctgaggt agggcgttcg
     4201 agtcgcctcg tctccaccac gtttcgtatg cgtaaagtcg ttgcggtata ctcttatgta
     4261 tatatgcgcg gctttttctt ttacctacgg cgctttatgg tcgcgatgga gggagcggca
     4321 tgatccgtgg cttcctgagt ctataccgac cacgttatgc ccgggtcatc gtccggctac
     4381 tgcaagacaa tcagtaccgt gcctgggatt atctgaaggc ctactggcac acggccgatt
     4441 tctcgcgcgc aagttatctg cacgacttcc aggatacggc agcgaaccgg atactgcgcc
     4501 gggcggttgc tgtcggcatg atcgtgcaac tgctggtggc agcttgggtg gcggtgcttt
     4561 ggcattggga aggattgtcc ggcggcagtc tgttcgccct ggccttgctg gtgtcctatc
     4621 cggtggtctg ggcgcatgtg cttgcgctgg tagccggggt gcgtaatgcc atccgcctgc
     4681 tggctcaccc aaaaaagctc gggcggtcga ttgtctgtcg tattctggaa ggccaggtgc
     4741 gtaaactgct caggcgtaac gaagtaaaga ttgtcgcggt ggctggcagc gtcggtaaga
     4801 ccagtaccaa attggcggtg gccaaattgc tcgaatgtca gttccgggtg cggttccaaa
     4861 acggcaacta caacgaccgc gtgacggttc cgctcgtctt cttcggacgc cgtgaaccga
     4921 atattttgaa catcttcgcc tggcttaaga ttttcgtggc caacgcccgc gccctcagcc
     4981 gaccgtaccc atacgatgtg gtggtcgtgg agcttggcac ggaccacccg ggcagcatga
     5041 tcgagtttgg ctatctcccg gtcgacatcg ctgtggtcac ggccgtcacc cccgagcaca
     5101 tggagtattt caagacgctc gacgctgttg ccgccgagga gctgacggtc ttcgacttcg
     5161 ccgataccgt actggtgaac ggcgatgccg tgccggccga gtacctggta ggccgcaaat
     5221 tccgcgaata cagtttaagg agcgaccagg ccaattactt tgcagccgtc accgatccga
     5281 gcctggcggg tcagaaactg cgtctgcgcc tggccaacgg ccacagtttg agtatggtga
     5341 gccgctacgt aggtgaaccg ggtgccagca ttgtcctggc ggcggccagc gtcgccgatc
     5401 agctcgacgc cgagcactcc gaaatagcca agggcattgc caatcttgaa cccttcgcgg
     5461 gccgcatgca gctattgaac ggccaaaaag acagcgtact catagacgac acctacaatg
     5521 ctagccccgt agcggtagaa gcggccctcg atgtcctgta cgcgcagccg gccaagcagc
     5581 gcatcgccat cttaggcagc atgaatgaac tgggagatta cagcccggcg gctcacaaag
     5641 aagtcggaaa gtactgcgac ccgaagaagc tcgacctggt ggtaaccatc ggtcatgatg
     5701 ccaaaaaata cctggcgccg gccgccaaaa aagccggttg caccgtccac acctttctga
     5761 gcccgtacga agccggtgat ttcgtgctgt ccaagttgcg caagggggca ttagtgctgg
     5821 ccgaaggctc acagaacggc gtctttgccg aggagtccct gaagccgctc ctggccgacc
     5881 cggccgacgc cgacaagttg gtgcggcaaa gcaaatactg gctcaagaaa aagcaacagc
     5941 agttcagacg ctaagtgcca tctcgaccag ccggcggcag agtgatggca tatccagccc
     6001 agctgaggcg gccatttttg gaaacaaact ttgggcggtc atacctggga tggtgtttgt
     6061 ctccaagata tagagcctgt ctgacgcccg atcatacatg atgtcggtgc gcgaaaagtg
     6121 acgacaaccg gtcaaactgt gagcttgcaa cgccaactcc tgagctctgg cctgtacttc
     6181 tgtatcgata tgtcgcggag ggcagagttc ctggctggca ccgttatatt tattttcgta
     6241 atcaaattct cctgtcgccg ggggtatgat ctcgatgacg ggcaacgctt cattgcccaa
     6301 taccccaacc gtcagctcag agccctctac caacgcctcc atgagcagct ccggatgccg
     6361 gctaaatgaa tcggctatgg ccgtttgggg aacctgggtt acatctcgga cgatgtgcgt
     6421 atcgatgctg gagccgccat cgactggttt gagtacgaac ggcttggcag cgagagggtg
     6481 ggtacgataa gtgtttacct tcacgagcgt ggcttcagcc ataggtaagc cggcaggaac
     6541 gcccttttgc cggtaggccc acttatcaaa acacagtgcc gatgcgctgg aacccgagcc
     6601 aacaaacttg gcgctactgg cttccagctg tcgctgcagc acaccgtctt cgccgccctt
     6661 accgtgcaac accgggaata cgacgtcgca agcaatgatt gcggccatgt cagcatccgc
     6721 aaggtcaaac gtttccacca caaaaccggc acgcgataag gcaacggcaa cggcatttcc
     6781 cgaacgtaac gacacctcac gttccgatga tgtcccgcca cacaaaacat gaacttttgt
     6841 catgtaagca gtataccctt ttgctcatta cgctcttgct tgcattcaaa cagaggggca
     6901 aggataatcg ccacatatgc atagccttga aaccatcccc gtgtatgagt ctgatcgcag
     6961 cccaaccggt gatcagttga agagccgtta cgatatttat gctgaccgca tcgaaattta
     7021 tgacatcgtt tggggtgatg aaagctttga cagcccttta ttcgtcgagt tggctaacca
     7081 tgatgccctg cgacgtctgc aaacggtgga gcagctgact ctgcctgata gatacaagac
     7141 aataccgggc tcgacgtact tttctcgctg ggagcatgcc tggggttcag cgatcttcgc
     7201 caaacgattg gcacaagaaa tgggttacgg gcaacgtgaa gtcgaggaaa tcgccgcccg
     7261 ggtcctactg tccgatgtct gtcacgctgc tcactcacat gccttggact ggatagcgca
     7321 gggtgccggg cagcttgaaa cgctgcatga tgagcgacgc gaatcgtacg ccgaagccgt
     7381 cggtattaac gagatactgc gtcgtcacgg ctttgatcca cagcggatac tccgcgaaga
     7441 aaaagacggg atatttgatg cagttatgcc cgaactcgat gtggacaggg ttgactacac
     7501 tttacgtgaa tcgtaccgct gggtagcgca gattccggaa tatcgttcat tgcttaacaa
     7561 ggacagcttc acggttaaaa atggccggct ggtctgccgc aataagacgg ccgccaagtt
     7621 gttcggcata tcatatacgc tgctggcaac ggaacactgg caggaaccgg cccaccgtct
     7681 acagttggaa ctgttcatgg aatccatgaa gcggattttt gttgcccggg acggccgccg
     7741 ggaagatgtc ggcacatact caccgcttga ttatctgatg gtgacggacg atacgctgca
     7801 gcgcttggca aatgaccacg acgagtacct gccgatgatg catgaattga tgcgcggcgt
     7861 cagcgaatcg gaatccaata gtcgatggcg ggcacgtgct gaaagggccc gagcagcact
     7921 gacgaccggt ctcggcgggg catcatcttc tatcgaatgg ataacagcgc actacgacac
     7981 cttgccgcgc tcatatgaga tatatccggc gcatgagtcc agattgcgca acggacggta
     8041 tgccacgctc atcatgctcg acaagctccg taagcggtcc gtagacccct tatatatcga
     8101 tgaggcggct gaggtgtgcc gtttgagcga gacagacgaa gcctaccggg agtacgaagc
     8161 ccgagccatc gggaacgtac agcaagactg gcgcgccggg attatcggta accaggcgtc
     8221 gaccatggcc ttaaaagcct gcctaaaaga aaaccgtgcg gtgtggccag gcatcatggc
     8281 aaggtcgcgg atgcctaatt ctgtccttcg tgagctgtta cggcaaacag tccgcaccag
     8341 taacggagcc gcagccaaag ccatcgattg ccggatacat agcgcctgaa gccgaattcg
     8401 cctccgctgc tcacctctgt tataatatcc tgcgatgaag cgatacaatc cgaaagacat
     8461 agaaccgcgc tggcagcagt attgggacga gcagcagact tacaaagctg atttgaacag
     8521 cgacaagccc aagttcatga gcatgagcat gttcaactac ccgagcggtg ccggtattca
     8581 cataggccac gccatgaact acaccatcag cgacgtgctg gccaggttca aacgccagca
     8641 gggctacgaa agttaccatc cggtaggctg ggacgctttc ggcttacctg ccgaaaacta
     8701 cgccatcaaa tccggcgtgt caccgcaggc ctcgatggcc aagatcatcc cgatgtacca
     8761 caaacagtac aaagccatgg gctggagcaa tgactggtcg aaggaaatag ccacccacga
     8821 gccgtcctat tacaagtgga cgcagtggat cttcagctcg atgtaccgcc acgggctggt
     8881 ataccaggac gcccgcatgc aatggtggtg ccctatcgac aataccgtgc tggccaacga
     8941 gcaggtgatt gacggcaaat gctggcgcca cgacggtccg aacgatccgc cggccgaaaa
     9001 gcgcgaggtc aagcagtggt tcttca
//
LOCUS       DASSFD010000073         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_127455, whole genome shotgun sequence.
ACCESSION   DASSFD010000073 DASSFD010000000
VERSION     DASSFD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127455"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..526
                     /locus_tag="VFH39_01630"
     tRNA            437..526
                     /locus_tag="VFH39_01630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:471..473,aa:Ser,seq:cga)
     gene            614..1012
                     /locus_tag="VFH39_01635"
     CDS             614..1012
                     /locus_tag="VFH39_01635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET6924513.1"
                     /translation="MQDSIFTMIIKGEIPCHKVYEDESTFAFLDIHPIQPGQVLVVPK
                     KQVGFIWDLEAADYQALMSTVQKVGRRLREVFPERQRVGVMVEGLDVADHAHIKVFPF
                     DTEREYRASPDMAVEPDHDALARLAQRIAF"
     gene            1039..1221
                     /locus_tag="VFH39_01640"
     CDS             1039..1221
                     /locus_tag="VFH39_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924514.1"
                     /translation="MSIFVIALVLGLGAGGWLFFQLNHRTGDANSGSNVVGAAVAALI
                     VFLIVFTLLKYVLHLH"
     gene            1270..2118
                     /locus_tag="VFH39_01645"
     CDS             1270..2118
                     /locus_tag="VFH39_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6924515.1"
                     /translation="MVMDEVGLGKRLQQVRRDAGYTQQQLCVQANLSYSTLAKIERGA
                     IKAPSIFTIQSIATALGISLDELLGLETHRHSTGGRKQTSKSGVRFVFFDLNDCLIRF
                     HTSAFTALARDSGQPVDVVESVFWKYDALVNRGEVTLDELNTIWAQRLGIMVDWKRYY
                     LNAVDPMPGMEELVRWVADRYYVGILSNTSPGFIHALRERGLLPDVHYDAIVDSSEVG
                     ALKPEPRIYEAAAAKASLAPEEILLIDDSRPNLIAAENLGWHTLWFDAYNPEDCIETI
                     RTALEA"
BASE COUNT          444 a          608 c          588 g          488 t
ORIGIN      
        1 gctacgggtg cccagtaatg agacaaacct acggtttttc tatagcttct cgcggtgaca
       61 tcctaactcc ttatatggag ggtatgatcc cggttcggca tatagactca ctgaacgttc
      121 gtctatcatc gcgcggggtc gcggaacgaa tccgctgact ccggctgctc ttttcccttc
      181 gtgcgaggta cgctgacggt tagttcagaa taatccacgt gccagaaaga aaacaacagg
      241 ggtaagttta gcataaaacg ttccttgatg ccagtccctc ccaattgtgt attcatctgt
      301 taaaattatg tgaacggaga tgcgcagcag cctcggaatc ggctgggctg agtaaacgac
      361 cggaccccgg ccacgcgccg gaaaagccaa ccggccggca ggccatagtg ccgctgcagc
      421 aatgccgtca tattcaggag aggtgcagga gtggttgaac tggcttgtct cgaaaacaag
      481 tgagccggca acggttccga gggttcgaat ccctccctct ccgccagatt aaatgtccca
      541 ccggtaaggt ggggcattta attagggggc ggtgaatact gcccctattc cttcgccaag
      601 gtatacttga attatgcaag attccatctt caccatgatc atcaagggtg aaatcccctg
      661 tcacaaggtt tacgaggacg agtccacctt cgccttcctc gacatccacc ccatccagcc
      721 cggccaagtg ctggtggtgc cgaagaaaca ggtcggcttc atctgggact tagaggctgc
      781 cgactaccaa gcgctcatgt ccaccgtaca aaaggtgggc cgccgcctgc gggaggtatt
      841 tccggagcgg cagcgcgtcg gtgtgatggt cgaggggctg gacgtggccg accacgccca
      901 catcaaggtc ttccccttcg acaccgagcg tgaataccgc gcctcaccag atatggcggt
      961 cgagcccgac catgatgccc tcgcccgtct ggctcagcga atcgccttct aggtgctccg
     1021 ctctgctaca atacccttat gtctatattc gtaatcgcac tggtactggg cctgggcgcc
     1081 ggcggctggc tgttcttcca gctcaaccat cgtaccggtg atgccaactc cggtagcaac
     1141 gtggtcggcg cagcggtggc agcgctcatc gtattcctga tagtgttcac cctactgaag
     1201 tacgtgctcc acctgcacta gccatttaat accatttctc ttccctatcg gcgcctaaac
     1261 tagtattata tggttatgga cgaagtgggg ttgggcaagc gcctgcaaca ggtccggcgc
     1321 gatgccggct atacgcagca gcagctgtgc gtacaggcga acctcagtta ttcgacgctc
     1381 gcgaagatcg agcgcggtgc catcaaggca ccatcgatct tcactattca aagtatcgcc
     1441 acggcgcttg gtataagctt ggatgagctg cttggtctcg agacgcatcg tcattccacg
     1501 gggggccgca agcagacctc caagagcggc gtccgcttcg tattcttcga cctcaacgac
     1561 tgcttgatac gctttcatac ctcggccttt actgctctcg cccgcgacag cggccagccg
     1621 gtcgacgtgg tcgaatcggt cttctggaag tacgacgcgt tagttaacag gggtgaggta
     1681 acgctggatg agctcaatac catctgggcg cagcgccttg gtatcatggt ggactggaag
     1741 cggtactatt taaatgccgt cgatcctatg cccggcatgg aggaactggt tcgctgggtc
     1801 gcggaccgct actatgtcgg tatcttgtcc aacaccagcc ccggctttat tcacgcgctt
     1861 cgcgagcgcg gtttgctgcc agatgtgcat tatgatgcca ttgtcgattc atcggaggtc
     1921 ggtgcgctga agcctgagcc gcgtatttac gaggcggccg ctgctaaggc ttcgttggcg
     1981 cctgaggaga tcctgcttat cgacgatagc cggccaaatc ttattgcggc cgaaaacttg
     2041 ggctggcaca ctttgtggtt tgacgcctac aatccggaag actgtatcga aaccatccgg
     2101 accgctctcg aagcgtaagg cccttatc
//
LOCUS       DASSFD010000074         9960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1221785, whole genome shotgun sequence.
ACCESSION   DASSFD010000074 DASSFD010000000
VERSION     DASSFD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221785"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFH39_01650"
     CDS             <1..439
                     /locus_tag="VFH39_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6924516.1"
                     /translation="WAINRDPNHFFYKTETETELHIVPADAAVAFLRDNPDVMYDLLA
                     RIYKGTEGLLGRLVHLMSGTAQSRLMYELILEGRRFGQAEQDGSVVLAINEGDLAART
                     GLSRETVSREVSKLKDRNLVTIAPKNLRILDMSALEQALGGEV"
     gene            complement(441..971)
                     /locus_tag="VFH39_01655"
     CDS             complement(441..971)
                     /locus_tag="VFH39_01655"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6924517.1"
                     /translation="MFKAKRTYRHPPDRNLGDRQQRMYDFMRENPVGVLSTVGPDGDP
                     HGSVIYYGVDDALTVTFLTRLNTRKFDNLRRQPRVMLTVCDPETQAVLQVRGEAEQVA
                     DSEEINAVAARTLGASLHTSEYGLPPISKLEAGPFVGVRIIPKQLRMAVFARPDAGDY
                     SDLFESVESFELPVEP"
     gene            1112..1624
                     /locus_tag="VFH39_01660"
     CDS             1112..1624
                     /locus_tag="VFH39_01660"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6924518.1"
                     /translation="MAQPNLSGYSQLPVKPSSTVQHAVTKGRTEEVRTQLQQAGADWY
                     DLKLPETDALPTVMRPAERVLGVVYGRYHHLAFEGEQVSRGMLVATDQRLLLLNKKPL
                     FIRCDEIMYEVVSSVSYAQAGFGGTVTLHSKIGDIMVRTFNQHCAVGFISALEGRLFA
                     LRVGQEVFHG"
     gene            1617..1997
                     /locus_tag="VFH39_01665"
     CDS             1617..1997
                     /locus_tag="VFH39_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924519.1"
                     /translation="MAKLIRFGRYRLIETRHQNKILYLGRSVYAWVQPVEFGEMLVTT
                     HKPHKTDCVLSIGLYRLYDNSDVHEVPTVQHLELEVGKDEWQGYILPGGLPRAGRPRA
                     RIVPTPQLVGARPRPTNAAGKVQL"
     gene            1994..2224
                     /locus_tag="VFH39_01670"
     CDS             1994..2224
                     /locus_tag="VFH39_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6924520.1"
                     /translation="MNTRRRLYRSDTDKRLSGVCGGIGEYFDIDPTLVRLAWVVLTVL
                     SGIVPGIIAYIIMAVVMPHRPSETAEDETGQA"
     gene            2205..2600
                     /locus_tag="VFH39_01675"
     CDS             2205..2600
                     /locus_tag="VFH39_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924521.1"
                     /translation="MKPGRRKLVLGLLAFNGLTAIVGGMALVAGINTPPIEWLDGTVF
                     ADYVVPGIILTFIVGGSAAIAAWYIWWRQPAGSLLGVLAGSIMCCWVLGEMAVVQHYS
                     WLQLLYLLTGGLLIGLDYTDAKDHFLQPH"
     gene            complement(2652..3392)
                     /locus_tag="VFH39_01680"
     CDS             complement(2652..3392)
                     /locus_tag="VFH39_01680"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HET6924522.1"
                     /translation="MPGIIEAPRSIVLAADMAPVAYEPIVRDLAGVEGLGGIKLGFKI
                     GYSRISLPGAVDIVREVDPDLKVIFDHQKAGTDIIDTGKDFAEAMTEAKVDAAILFPF
                     TGPKVQHNWVRELQKKNIPAICGAEMTHEGQVAESDGTQGGYIHPRAFERIFTLAVQL
                     DVRDFVVPGNKAERVAHYRTFIGSLLGQEEFTLWAPGFVTQGGDVSETGAAAGPNFHA
                     IVGRGIYGAEQPRVAAMALGQKLLGRNR"
     gene            3485..3907
                     /locus_tag="VFH39_01685"
     CDS             3485..3907
                     /locus_tag="VFH39_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET6924523.1"
                     /translation="MAENKLCPFCNASERVLKQNELAQVVLSNPHKVPGHFLVMPMRH
                     IEQPWQLTPEELQAVFDLIFFVEQRVVSKLGDGVDIRQNYRPFKAADELKVNHVLFHV
                     LPRSDNDYLYQVSEQYEKDLFADLDELEAKEVAKLLED"
     gene            3998..4504
                     /locus_tag="VFH39_01690"
     CDS             3998..4504
                     /locus_tag="VFH39_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6924524.1"
                     /translation="MKQSAGILAYRRKAAEVEVLLLHPAGPYWAHKDFWHFPQGELDE
                     GEDSLAAAHREFLEEVGVPVPDGELYDLGVAKRPGKEYDMWALEADVDLSKFSDTLTT
                     NVFTMEWPPKSGQQQTYPENDRAEWFPLAVAHRKVFGYMQVFIERLADHLGVEVGEAP
                     ESGQQSLL"
     gene            4629..6089
                     /gene="metG"
                     /locus_tag="VFH39_01695"
     CDS             4629..6089
                     /gene="metG"
                     /locus_tag="VFH39_01695"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET6924525.1"
                     /translation="MKSYYYVTTSIPYVNAEPHIGFAMELLQADVLARYGRQQGKTVL
                     FSTGTDEHGGKIAEKAEEQGIKPQEFADRISQAFRDMTGLLNVSNDRFIRTTDKAHEQ
                     RAQLVWKSLSKDDIYKGKYTGWYCTGDEAFFTETEVKANNGVCPAHNRPYEKVEEENY
                     FFKLSKYSAAVGEAIEKDELHIVPDTRRNEILSVIKSGLEDISISRPKEKIPWGIPVP
                     GDDKQVMYVWFEALMNYITVLGYPEHEDFKNFWPADVQIIGKDIIRFHAAIWPAMLLS
                     LVLPLPQTLYVHGFVTSEGKKMSKTLGNVVAPKHLIAEFGVDAFRYFFLRHIPSHNDG
                     DFSRDRFVEAYNNELANELGNAVQRTLAMIVKYQGGLIGDIPDGEHDTAQYREALAKC
                     QFDRALDEAWEQVRGLNQYIDTEKPWEIAKSGDDDHLREVLAYQVSNLLEIADLLEPF
                     LPATAVAIRNIFNTGVVRPPEGTLFPKHEAAKPAEA"
     gene            6095..6922
                     /locus_tag="VFH39_01700"
     CDS             6095..6922
                     /locus_tag="VFH39_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6924526.1"
                     /translation="MLDTSKLFDTHCHVHETVKPVTPVYDKWHDKGAKSDPDEIIAAA
                     RDAGVGYMLCIGTTPADSELAVDFVQNRPNMWASIGIHPHEAAAALQNGTLDAFAALV
                     RRPKVVAVGECGLDYFYNHSPKEAQLEVLRFQIELALKHDLPLSFHVREAFDDFWPVF
                     ESYKGIRGVLHSYTDNLENLERAVSHGLYVGVNGIATFAAKDEQKRVIYRTIPQRLLL
                     LETDAPFLTPVPFRGTICEPKHVAVTAEFVSKLRDEPLAQMIAATTDNAGTLFNINI"
     gene            6966..7412
                     /locus_tag="VFH39_01705"
     CDS             6966..7412
                     /locus_tag="VFH39_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924527.1"
                     /translation="MVNKLFGNSESARGRVDSVLAEMSVGMGEQLRHAVHAVQSVMYS
                     QYPEQARAIDAPVNHVSHIATKAVAYGNQAPAPKVETPAATAVEAAPVNEQASTAVRD
                     FLQEDVPTTPPPYLNVIAEKQPDDPVEAARLATRAVYDEVALNQRG"
     gene            7415..7789
                     /locus_tag="VFH39_01710"
     CDS             7415..7789
                     /locus_tag="VFH39_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924528.1"
                     /translation="MGLLTASRQRAQQQKRADAHRSLLHYGARVGGDVFGPIPKGHRR
                     EFFCLDERTWVWHEEWTDPTTKQYHVVTTRYDVQPQAILKSQGSNSYQKLSPEDARNF
                     YQAVKLYGERFGRELDRIASGN"
     gene            7958..9166
                     /locus_tag="VFH39_01715"
     CDS             7958..9166
                     /locus_tag="VFH39_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET6924529.1"
                     /translation="MDSPLIYMDYAAATPVHERVLKAMQPFYTERFYNPSATYLPARE
                     VSAELQAARATVARWLGAKPAEIIFTAGATESTNLAIHGVMQQFDGDEDQRGNIVISA
                     IEHPAVLEPALRYDCRTVPVDAQGLLDLKALEAAIDEHTVLVSVMYANNEVGTIQPIK
                     DIAGIVAKKRRERQQAREDELGTWNLEAGAPGQPYPLYLHTDAAQAGNYLDLHVSRLG
                     VDMLTLNGGKLYGPKQSGVLYLAGHVRLRPLIVGGGQESHVRSGTENVAQAIGFAAAL
                     DIAQSLRQEEVSRLQVLQQDFMAGLEAALPGVVVNGSRKKRLPNNVHVTFPGQDNERL
                     LLELEQRGILAAAGSACAASDEEPSHVLRAMGVGDADARASLRFTLGRQTRAKEIKQA
                     LTALTDILRA"
     gene            9205..>9960
                     /locus_tag="VFH39_01720"
     CDS             9205..>9960
                     /locus_tag="VFH39_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924530.1"
                     /translation="MLTLIRHLRRSLALLFVAAALLLPAAAQAASSSKTTKSSTASGS
                     GTDSANNQVTQGYGTSQPLQKGMIVKLVDKDPTKVEAVTTATVAKMQGVVVAANDAAF
                     ALGNSANAGQVFVATTGHYDVLVSNQNGPIKSGDYITISALAGIGMKDNATAAAVLGR
                     ATAAFDGSSNVSGTATLKDSTGRQVKVSIGRIPVDINAGHNPLQQTGEKSVPGFLRTA
                     AQLVSGKPLNATRIYLGLFVLVATTLISAIMLFS"
BASE COUNT         2133 a         2978 c         2946 g         1903 t
ORIGIN      
        1 ctgggctatc aaccgcgacc cgaatcattt cttttacaag accgagaccg agacggagct
       61 gcacatcgta ccggccgatg ctgccgtggc tttcctccgg gacaatccgg atgtcatgta
      121 cgacctcctg gcgcggatat acaaaggtac cgagggctta ctgggccggc tggtgcacct
      181 catgtcggga acggcgcaga gccgtttgat gtacgaactt attttggaag gccgccgttt
      241 cggacaagct gaacaggacg gcagcgtggt gctggccatc aacgaaggtg acctggcggc
      301 gcgcacgggg ctgagccggg aaacggtcag tcgcgaagtc agcaaactca aagatcgtaa
      361 cctggtgact atcgcaccga aaaatctgcg catcctcgat atgtccgcgc tcgaacaggc
      421 gctcggcggc gaagtctagg ctacggttcg accggcagct cgaacgactc aaccgattcg
      481 aacagatcac tgtaatcgcc ggcgtcgggt cgcgcgaaca cggccatccg caattgtttg
      541 ggaatgatcc taacgcccac gaacgggccg gcctccagct tgcttatcgg cggcagcccg
      601 tactcgctgg tgtgcaggct ggcccccaag gtgcgcgcgg cgaccgcgtt gatctcttcg
      661 gaatcggcaa cctgttcggc ctcgcccctc acttgcagca ccgcctgggt ttccggatca
      721 cagacggtca gcatgactcg tggttggcgg cgcagattat cgaacttgcg ggtattaaga
      781 cgggtcagga acgtcacggt cagggcatcg tcgacgccat agtaaatgac gctgccgtgc
      841 ggatcgccgt cggggccgac ggtactcagg acccccaccg gattctcgcg catgaaatcg
      901 tacatacgct gctgtcggtc accgagattc cggtctggtg ggtgtcggta tgtgcgcttg
      961 gctttgaaca tgtcgttcgg atctccttcc tgctctcagt ctgcgccggg ctacgccggc
     1021 aagctagggc gcacatcacg tgaccaatgt gatgtgggtc aaggcgcttt taggccggat
     1081 ggtcttatat ggaagcagga ggaatatggt gatggcgcaa ccgaaccttt ccggctactc
     1141 gcaactaccg gtgaaaccca gtagcacggt gcagcatgcg gtgacgaaag ggcggacgga
     1201 ggaggtgcgg acgcagctgc aacaggccgg cgccgattgg tacgacctga agctgccgga
     1261 aaccgacgcc ttaccgacgg tgatgcgccc ggccgagcga gtgctgggcg tggtgtacgg
     1321 ccggtaccac catctggcgt tcgagggcga gcaagtcagc cgtggcatgc tggtagcgac
     1381 cgaccagcgg ctcttgctgt tgaacaagaa gccgctgttc atccgttgtg acgagatcat
     1441 gtacgaagtg gtaagctccg tttcttacgc ccaagccggt ttcggtggga cggtgacgct
     1501 gcacagcaag ataggtgaca tcatggtgcg gactttcaac cagcattgtg ccgtcggctt
     1561 tatatcggcc ctggaagggc gcttgttcgc gctccgggtc ggtcaggagg tcttccatgg
     1621 ctaaattgat ccgtttcggg cgttaccggc tcatagagac ccggcaccag aacaagatcc
     1681 tgtacctcgg ccgctcggtg tacgcctggg tgcagccggt ggagttcggg gaaatgctgg
     1741 ttacgaccca caaaccgcac aagaccgact gcgtgctgag catcgggctg taccggctgt
     1801 atgacaattc ggacgtgcac gaagtgccga ccgtccagca cctcgagctg gaggtcggta
     1861 aggacgaatg gcagggctac atactgccgg gcggcttgcc ccgggcgggg cgtccgaggg
     1921 cccgcatcgt gcccacaccg caacttgtcg gcgcccggcc gcgaccgacc aatgccgcag
     1981 gaaaggtgca gttatgaaca cgcgccgacg cctctatcgc tccgacaccg acaaacgact
     2041 gtccggcgtc tgcggcggca tcggtgaata tttcgatatc gacccgacgc tggtccggct
     2101 tgcctgggtg gtgttgaccg tgttgagcgg catagtgccg ggcatcatcg cctatatcat
     2161 catggcggtg gtcatgccgc accggccgtc ggagacggca gaagatgaaa ccgggcaggc
     2221 gtaagctggt tctcggcctg ctggccttca acggcctgac ggccatcgtg ggcggcatgg
     2281 cgctggtcgc gggcatcaat acgccgccca tcgagtggct ggacggcacc gtctttgccg
     2341 attacgtcgt gccgggcatc atcttgacgt tcatcgtcgg cggcagcgcc gcgatcgcgg
     2401 cctggtacat ctggtggcga cagccggcgg gctcgctgct cggcgtgctg gcgggcagca
     2461 tcatgtgctg ctgggtcttg ggcgagatgg cggtggtcca gcactactcc tggctgcagc
     2521 tgttgtatct gctgacgggc ggcctgctca tcggcctcga ctacaccgat gccaaagacc
     2581 acttcctgca accccactag ctctactgag acgaagcaat actacccact gaccaatggg
     2641 atatagtgcc cttaccgatt ccttcccagc aatttctgac cgagcgccat ggcagccacc
     2701 cgcggttgct cagctccgta tattccccga ccgacgatag catggaagtt aggcccggca
     2761 gctgcgcctg tttcagaaac atcgcctcct tgtgttacaa agcctggcgc ccataaggtg
     2821 aattcttcct ggccgagcaa actgccaata aatgtacgat agtgcgccac acgttccgcc
     2881 ttattgccag gtacgacgaa atcacggaca tccagctgga ccgctaatgt aaaaatccgt
     2941 tcaaacgccc gaggatggat ataaccccct tgcgtcccgt cactttcggc aacctggcct
     3001 tcgtgcgtca tttcggcgcc acagatcgcc ggtatgttct ttttctgaag ctctcgcacc
     3061 caattgtgct gaactttagg gccggtaaac ggaaagagga ttgcggcgtc cacctttgcc
     3121 tcggtcattg cttcggcgaa atccttaccc gtatcaatga tgtctgtccc tgccttctgg
     3181 tggtcgaaaa taactttaag atccggatct acttcacgca cgatatccac cgctccaggc
     3241 agactgatgc ggctgtagcc tatcttgaaa ccaagcttta tacctccgag accctcaaca
     3301 ccagctaaat cccggacgat cggttcatac gcaacgggag ccatatcggc cgcaagtacg
     3361 atactccgtg gtgcttcaat gattcctggc ataaaccctc atttctcccg ccctgctagt
     3421 tattgccagt cagtatatag cacccggctg gccaagtgta cggctaatcg ctacactgta
     3481 aggcatggca gagaacaaac tatgtccctt ctgcaacgcc agcgagcgtg tgctgaagca
     3541 gaatgagctg gcgcaggtgg tcttgagcaa tccgcacaag gtgcccggcc acttcctggt
     3601 catgcccatg cggcatatag agcagccctg gcagctgaca ccggaggagc tgcaggccgt
     3661 gttcgacctg attttctttg tcgagcagcg ggtggtcagc aagctgggcg acggcgttga
     3721 catccgccag aactaccggc cgttcaaggc cgccgatgag ctgaaggtga accacgtgct
     3781 gttccacgtg ttgccgcgca gcgacaacga ctacctgtac caggtgtcgg agcagtacga
     3841 gaaagacctg ttcgccgacc tcgacgaact ggaagccaaa gaagtcgcaa agctgctcga
     3901 ggactgatta cgcagctgcg ccacaccagg caatcgatcc gccaaaacgc taattttaca
     3961 gagaacagag taaacttaac atgctaagta acaacgaatg aaacaaagtg ccggcatcct
     4021 ggcctatcgt cgcaaagcag ccgaagtcga agtgttgctg ctccaccctg ccggccctta
     4081 ttgggcacat aaagactttt ggcacttccc gcaaggagaa ctggatgaag gcgaagacag
     4141 cctggcggcc gctcatcggg agttcttaga agaagtcgga gtaccggtac cggacggtga
     4201 gctgtacgac ctcggggtag ccaagcgtcc cggcaaagag tacgacatgt gggccctaga
     4261 agccgatgtc gacctaagca agttctccga caccctcacg accaacgtct tcaccatgga
     4321 atggcccccg aagtccgggc agcagcagac ttatcccgag aatgatcggg cggagtggtt
     4381 cccgttggcg gtcgcccacc gcaaagtatt cgggtatatg caggtattca tcgaacggct
     4441 ggccgaccac ctgggggtgg aggtcggcga agctcccgaa tctggccagc agtcacttct
     4501 ctagggtata attgaccggt atgacaagcg tgacgaacct tcctgacttt cgtatgatgc
     4561 cccagcgggc ataattttca cgctcccaaa ctcttctctt atcactgtaa acaggtacac
     4621 ttgaattcat gaaaagctac tactatgtca ccacctccat tccgtatgtg aacgccgaac
     4681 cgcacatcgg cttcgccatg gagctgctgc aggccgacgt gctggcgcgg tatggccgcc
     4741 agcagggcaa gacggtgctg ttttctaccg gcaccgacga gcacggcggc aagattgccg
     4801 aaaaagccga ggagcagggc atcaagccac aggaattcgc cgatcgtatc agccaagcct
     4861 tccgcgacat gaccgggctg ttgaacgtca gcaacgaccg cttcatacgc accaccgaca
     4921 aagcccacga gcagcgcgcc cagctggtct ggaagagcct gtccaaggac gacatctaca
     4981 agggaaagta caccggctgg tattgcaccg gcgacgaagc gttctttacc gagaccgaag
     5041 tgaaggccaa caacggcgtc tgcccggcgc ataatcgccc gtacgagaag gtcgaggaag
     5101 aaaactactt cttcaagctc agcaaatact cggcggccgt cggcgaggcg atcgagaaag
     5161 acgagctgca catcgtgccc gatacccggc gcaacgaaat cctgtcggtc atcaagtccg
     5221 gcctggagga catcagcatc tcgcgtccga aagagaaaat cccctggggc atcccggtcc
     5281 ccggtgacga caagcaggtg atgtacgtct ggttcgaggc gctcatgaac tacataaccg
     5341 tgcttggcta cccggagcac gaggacttca agaacttctg gccggccgac gtgcagatca
     5401 tcggcaaaga tatcatccgc tttcacgccg ccatctggcc ggccatgctg ctgagcctgg
     5461 tcctgcccct gccacagacg ttgtacgtgc acggcttcgt gacgtcggaa ggcaagaaga
     5521 tgagcaaaac gctcggcaat gtggtggccc ccaagcacct catcgccgag ttcggcgtcg
     5581 atgccttccg ctacttcttc ttgcgccaca tccccagtca caacgacggt gacttcagcc
     5641 gcgaccggtt cgtggaggcc tacaacaacg agctggccaa cgagctcggc aacgctgtgc
     5701 agcgcacgct ggcgatgatc gtcaaatacc agggcggtct catcggcgac atcccggatg
     5761 gcgagcacga caccgcccag taccgcgagg cgctagctaa gtgccagttc gaccgggctt
     5821 tggacgaggc ctgggagcag gtgcggggcc tgaaccagta catcgacacc gaaaaaccgt
     5881 gggaaatcgc caagtccggc gacgatgacc acctgcgcga ggtgctggct taccaggtaa
     5941 gcaacctgct ggagatagcc gacctgctgg aaccgttcct gccggctacc gccgtcgcta
     6001 tccgcaacat cttcaacacc ggcgtcgtcc ggccgcccga aggcacgctg ttccccaagc
     6061 acgaagccgc caagccggcc gaagcctgaa actcatgctc gatacctcaa agctgttcga
     6121 tacgcactgc cacgtccacg agacggtgaa gccggtgacg ccggtgtacg acaagtggca
     6181 tgacaagggt gccaagagcg acccggacga aatcatcgcg gcggcgcgtg atgcaggcgt
     6241 gggctacatg ctgtgcatcg gcaccacacc ggcggacagt gagctggccg tggacttcgt
     6301 ccagaaccgc cccaatatgt gggccagcat cggtatacac cctcacgagg cggctgcggc
     6361 cctccaaaac ggcactctgg acgcatttgc agcccttgtg aggcgtccga aagtggtagc
     6421 agtgggtgag tgcggtctgg actacttcta taaccactca ccgaaggagg cgcagttgga
     6481 ggtgttgcgc ttccagatcg agctggcgct caagcatgat ttacccctgt cgtttcatgt
     6541 gcgggaggcc ttcgacgact tctggccggt gttcgagagc tataagggca tccgcggcgt
     6601 actacacagc tatacggata atcttgagaa cctggagcgg gcagtcagtc acgggctgta
     6661 tgtcggcgtc aatgggattg ccacgttcgc ggccaaagat gagcaaaagc gtgtgatata
     6721 tcgcacaatt ccacaacgat tactactgtt ggagacggac gcgcctttct tgactccagt
     6781 gcccttccgt ggtaccatat gtgagcctaa gcatgtagcc gtgactgctg agtttgtgag
     6841 caagctccgg gatgaaccgc ttgcacaaat gattgcggcc acgactgaca acgccggcac
     6901 actcttcaac atcaacatct gaaccgtcaa acatttattc aacgcgtaac gatcgaggga
     6961 tccacatggt caataaatta ttcggtaaca gtgagtctgc gcgcggtcgc gtcgacagtg
     7021 tgctggccga aatgtccgtg ggtatgggcg aacagcttcg ccatgccgtc cacgccgtgc
     7081 agtcggtcat gtattcccag tatcctgaac aggcccgtgc tatcgacgca ccggtcaacc
     7141 acgtcagcca tattgctacc aaagctgttg cctacggcaa ccaggcgccc gctccgaagg
     7201 tagagacacc ggcagccacc gctgtggagg cagcgcccgt caacgagcag gcatccactg
     7261 ccgtccggga tttcctgcag gaagacgttc ccacgacacc gccgccctat ctgaatgtga
     7321 tcgccgaaaa gcaacccgat gacccggtcg aagctgccag gttagcgaca cgcgccgtct
     7381 atgacgaggt agcgctgaac caaagaggat aatcatggga cttttaaccg ccagtcgtca
     7441 acgtgcacag caacaaaagc gggccgatgc ccatcggtca ttgctgcact acggtgcccg
     7501 ggtgggcggc gatgtgttcg ggcccattcc gaaaggacac cggcgcgaat ttttctgctt
     7561 ggatgaacgc acctgggtgt ggcacgagga atggaccgac ccgacgacca agcagtatca
     7621 cgtcgttacc acccggtatg acgtacagcc gcaggccatt ttgaagtcgc agggcagtaa
     7681 ctcgtaccag aaactcagcc ccgaggacgc gcgcaacttt taccaggcgg tcaaattgta
     7741 cggcgagagg tttggccgcg agctggaccg cattgcctcc ggcaattagc aaccacaccg
     7801 gcggcacata ccgggcgctc cgctgcgata cgaagctcac gcacctgaaa gtgcgctgcg
     7861 cttcgtttct cgcgtagcat cccggcctgc actcaccggt gaggctgcca gcttgttggt
     7921 aatcacctga aacggtaatt ggtatactga aagtcttatg gattcacctc tgatttatat
     7981 ggattatgcg gcagccacgc cggtgcatga gcgggtgctt aaagccatgc agccgtttta
     8041 tacggagcgg ttttacaacc cctcggccac gtatttgccg gcgcgggagg tgtcggctga
     8101 gctccaggcc gcccgggcta ccgtcgcccg gtggctgggc gctaaaccgg cggaaattat
     8161 ttttacggcc ggggcgacag aatcgaccaa tctggcgatt cacggggtta tgcagcagtt
     8221 cgacggggac gaagaccagc gcgggaacat cgttatcagc gccatcgagc acccggccgt
     8281 cctcgaaccc gcgctgcgct acgactgccg gaccgtgccg gtcgacgcgc agggtctgct
     8341 cgacctcaaa gcactcgagg cggcgattga cgagcacacg gtgctggtga gtgtcatgta
     8401 tgctaacaac gaagtcggga ccattcaacc catcaaagac attgccggaa tcgtggcgaa
     8461 aaaacggcgc gaacggcagc aggcccggga agacgagctg ggaacatgga acttggaagc
     8521 aggcgccccg ggccagccat acccgctgta tctgcacacc gatgccgcac aggccggtaa
     8581 ttatctcgac ctccatgtca gtcggctcgg cgtggatatg ctgaccttga acgggggaaa
     8641 actgtacggg ccgaagcaga gtggtgtgct gtacctggcc gggcatgtca ggctgcggcc
     8701 gctgatagtc ggcggcggcc aggagagcca tgtgcgcagc ggcaccgaaa acgtggccca
     8761 ggccatcggg ttcgccgcag cgctcgatat cgcgcagagc ctgcgccagg aagaggtgtc
     8821 gcggctccag gtgctgcagc aggatttcat ggccgggctc gaagccgcac tgccgggcgt
     8881 ggtcgtcaac ggctcccgta agaagcggct gcccaacaac gtccatgtga cgtttcccgg
     8941 acaggataat gagcgactgt tgctggaact ggagcagcgg ggcattttgg cggcggccgg
     9001 ctcggcctgc gccgcttcag acgaggaacc ctcgcacgtg ttgcgtgcca tgggcgtagg
     9061 cgatgccgat gcccgggcga gcctgcggtt cacactgggc cgccagacca gggccaaaga
     9121 gattaagcag gccctgacag ctctgaccga catcctgcgc gcctgagctc ctgccaggcc
     9181 gcttcgtgta tacttaggct caagatgctc acgcttattc ggcacctccg gcggtctctg
     9241 gcgcttctgt tcgtggccgc ggcattattg ctgccggcgg cggcgcaggc agccagcagt
     9301 tccaagacga ccaaatcctc aacggcttcc ggttccggta cggattccgc caataatcag
     9361 gtcacccagg gttacggcac cagtcagccg ctgcagaaag gcatgatcgt caaactggtc
     9421 gataaagacc cgaccaaagt ggaggcggtg acgaccgcta ccgtggccaa gatgcaagga
     9481 gtcgtcgtgg cagccaatga cgctgccttt gcgctcggca attccgccaa tgccgggcag
     9541 gtgttcgtgg cgaccaccgg tcactacgat gtactggtca gtaaccagaa cgggcccatc
     9601 aagtccggcg attatataac catttcggca ctggccggca tcggcatgaa ggacaacgcc
     9661 accgccgcgg ccgtattggg acgcgccacc gccgcgttcg acggttcatc gaatgtttcc
     9721 ggtacggcta cgctcaaaga ttccaccggt cggcaggtaa aagtatccat cggccgtata
     9781 ccggtcgaca tcaacgccgg tcataatccg ctgcagcaga caggcgaaaa gagtgtgcca
     9841 gggttcctgc gtaccgccgc tcagctggtc agcggcaagc cgctcaatgc cacccgcatt
     9901 tatctggggt tattcgtgct ggtcgctact acccttattt cggcaattat gctgttttcc
//
LOCUS       DASSFD010000075         7898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_896255, whole genome shotgun sequence.
ACCESSION   DASSFD010000075 DASSFD010000000
VERSION     DASSFD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896255"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFH39_01725"
     CDS             complement(<1..1539)
                     /locus_tag="VFH39_01725"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6924531.1"
                     /translation="MFVTGGVLSGLGKGITAASIGNLLKARGLTVNLQKCDPYLNVDA
                     GLLNPREHGECFVTKDGAETDLDLGHYERFIDEELTSASSVMSGKILLKLIQDERAGK
                     FEGDDVQIIPHLTNAIQDWIRQAGDGFDVHIVEIGGTVGDYESLSFIEAIRELGVRVG
                     LDNCVYVHVVYLPYLNASNETKTKPAQNSVRELRGLGISPDILVGRSEQLANDGVKRK
                     LSLFSGVDEEAIALLPNAPSIYQVPLTLEDTGIADVITEKLGLKAKKPDLKDWREVVE
                     RATTAHPRTVHIGFVAKYMDNTDTYMSVFEALKSAGWHNDTSVDITWIDASEFDAPDA
                     DIRGTLKKYDGIVVPGGFGQRGLEGKIKAAQYALTDKVPYLGLCLGMQMAVIAAARNA
                     GIEDATTFELNPDSPNVVINTMADQEGLHMTGGTMRLGNYDCQLEKGSLAEKVYGQRT
                     ITERHRHRGEANNAYRDRYESWGIRASGINPENNLVEIIEAIDHPFLLASQFHPEFKS
                     RPNRA"
     gene            complement(1642..2871)
                     /locus_tag="VFH39_01730"
     CDS             complement(1642..2871)
                     /locus_tag="VFH39_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6924532.1"
                     /translation="MRLRKKKVEGDEPVRVKSGLTGLLKGVLFGVIIVLVFGLGVNIG
                     NGRIHFGKSGQNGLPNQLDYSSVNTVYQALKENYNGKLTEQQLVNGMKHGLAEATNDP
                     YTVYFTPSEAKQFQGELNNTFSGIGAELGQDSDKNIIVISPISGFPADKAGLKPQDII
                     ATINGQSTAGMSVDEAVGKIRGPAGTTVKLGIVRDKTQTLTLSIIRQDIQVPSVTTKV
                     LDGNIGYMHISTFSDDTANLAQQAADKFKQQGVRGIVLDLRGDPGGLLDAALKVSSLW
                     VPEGKLIVSERGTIGTDDHFATGGNELGGIPTVVLIDGGSASASEITTGALHDNHEAY
                     VMGQKSFGKGVVQQLINLGGGAELKVTVASWYRPDGQNINHHGITPDQAVKAGLNDTL
                     GGTDAQLQAAEQYLAAH"
     gene            complement(3032..4207)
                     /locus_tag="VFH39_01735"
     CDS             complement(3032..4207)
                     /locus_tag="VFH39_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET6924533.1"
                     /translation="MPEKKKTSRKFVAWLRSTPVLVVASLLTVGMAVATPLVRADQYD
                     AQINALQQQNNAAQGNLNNLEAQASSYQDTINKLQSQINAIQAAIADNEAKQAQLQQQ
                     IVEDQKQIDYEKTVLANDVKTTYINGQITPVEMLATSSSLSDYIDKQEAYSVVQDKIQ
                     ATVQQITELQKKLQSQKEQVDQLLATQKNENDQLSAAKAQQDQLLSMNESQQASYNSQ
                     ISANQSKIAQLRAAQAAANRRLDSSGAVITSGSCGGSYPQTASGSYGPWGCGYVHSSD
                     YTPGCSYMDSWGMCNRECVSYTAWMVYKTYGINTIGFGNANQWPGSARAAHIPTGYTP
                     KVGSVAIYTGGTYGHAMWVVGVSGDQVHVYSYNDGYDGNFYDHWVNGSGLTYIYFGG"
     gene            complement(4553..5494)
                     /locus_tag="VFH39_01740"
     CDS             complement(4553..5494)
                     /locus_tag="VFH39_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET6924534.1"
                     /translation="MQRKYTTFMRIVHTGIVNFIRNASLAIAAMAVMIVTLTIVLFSL
                     IANATFSNTIAQITSKIDVSVYLKDSVTESQAKQLVAKLEKLPNTQSVTYLSKAQVLA
                     NYQRQNAGNQQLLTAVTETANPLPATIHIKPTDLNKLNDIKAYLERPDVTALESDPPS
                     YSGNLKQAIDNITHATNVLREIGIIAVIVFAVVSALIIFNTIQMAIFNRRDEIQIMRL
                     LGASTSYIRGPFIVESVIYGMLSAVISVLIINGVFVASSSTLQATTFGLLDIGYATTY
                     FDSHFWLFLTLQLAVGIIIGAVSSFIATRRYLRFKTK"
     gene            complement(5506..6189)
                     /gene="ftsE"
                     /locus_tag="VFH39_01745"
     CDS             complement(5506..6189)
                     /gene="ftsE"
                     /locus_tag="VFH39_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET6924535.1"
                     /translation="MILLDRVTKTYNKNLGPALDRVSLHVEPKEFVIVVGQSGAGKST
                     LLKLLTREERPSGGKIVIGGIDYDKLKDKDIPLLRRKIGVVFQDFKLLPNKMVFENVA
                     FALEIVGVSHKEILHTVPKVLDIVNLTGKEKRMPQELSGGERQRVAIARAIVRQPKIL
                     IADEPTGNLDPKHAWDVIKVLEKINKYGTTVLLTTHNVEIVNKLKRRVVTIQNGRIVG
                     DRANASYKI"
     gene            complement(6331..6714)
                     /locus_tag="VFH39_01750"
     CDS             complement(6331..6714)
                     /locus_tag="VFH39_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924536.1"
                     /translation="MSKANGFVTNSDGQDKLRVVVSGQTGGFIDDLIPTVKSHSMAEV
                     VRRSLMTFLGLVESNPNYRNLIEHPVPAHANATRNVSLQFKVSDDFRHVFEQLEKSEH
                     LTARQLVEYAVRWLWLSNRFMQRGG"
     gene            complement(6764..7849)
                     /gene="prfB"
                     /locus_tag="VFH39_01755"
     CDS             complement(6764..7849)
                     /gene="prfB"
                     /locus_tag="VFH39_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET6924537.1"
                     /translation="MDELLTKAKALQSDIRDAAERLKLFAHEKELDKLQAQSQQPDFW
                     QDNLAAQAVMKQIAKLEARTKPWRELLGAADELIDIIHLKVEGSEQEVTQQLDFITKK
                     FDEYKEQLKLNGPYDDHDAILSIHAGAGGTDAQDWAQMLFRMYQRYAEKAGWQTQLID
                     ESPGEEAGLKSVTLELAGPFAYGKLKGEHGVHRLVRLSPFNAESRETSFAKVEVTPKI
                     DAPDELEIDDKDLKVDVYRSGGHGGQSVNTTDSAVRVTHLPTGIVVAIQNERSQLQNK
                     ELALTILRSKLAQLQQEQHAEKVSELKGPNEEAAWGNQIRNYVLHPYKQVKDLRTSYT
                     STDPDQVLDGNLDPFIEAYLEANIAAN"
BASE COUNT         1361 a         2212 c         2371 g         1954 t
ORIGIN      
        1 cgcgcggttc gggcggctct tgaactcggg gtggaactgg ctggccagca ggaatgggtg
       61 gtcgatggcc tcgatgattt ccaccaggtt gttttcgggg ttgatgccgc tggcgcggat
      121 accccaggat tcgtagcggt cacggtaggc gttgttggcc tcgccgcggt gacggtggcg
      181 ttcggtaatc gtccgctgac cgtatacttt ttcggccagg gagccctttt cgagctggca
      241 atcgtagttg cccagccgca tggtgccgcc ggtcatgtgc aggccttcct ggtcagccat
      301 agtattgatg acgacattcg ggctgtccgg gttgagttcg aaggtagtgg cgtcctcgat
      361 gccggcgttg cgggcagcgg cgatgactgc catctgcatg ccgaggcaca gacccaagta
      421 cgggacctta tcggtaaggg cgtactgggc ggctttgatc ttgccctcga gcccgcgctg
      481 gccgaaaccg ccggggacga cgatgccgtc gtatttcttg agcgtgcctc tgatgtccgc
      541 gtccggcgca tcgaattcgc tggcatcgat ccaggtgatg tccacgctgg tgtcgttatg
      601 ccaaccggcg ctcttcagcg cctcgaagac ggacatgtag gtgtcggtgt tgtccatgta
      661 cttggcgacg aaaccgatgt gtacggtgcg cgggtgggcg gtggtggccc gttcaacgac
      721 ttcgcgccag tccttgaggt ccggcttctt ggccttgagg cctaacttct cggtgatgac
      781 gtcggcgatg cccgtgtctt cgagcgtcaa cggcacctgg tagatgctgg gggcgttcgg
      841 cagcagcgcg atggcttctt cgtcgacgcc gctgaacagg cttagcttgc gcttgacgcc
      901 gtcgttggcc aattgttcgc tgcggccgac caggatgtcc ggtgaaatgc ccaggccccg
      961 cagttcgcgg acgctgttct gggccggctt ggtcttggtc tcgttgctgg cgttcagata
     1021 cggcaggtac accacgtgca cgtaaacgca gttgtccagc ccgacgcgca cgcccagctc
     1081 gcggatagct tcgatgaagc tcagactttc gtagtcaccc accgtgccgc cgatttcgac
     1141 gatgtgcacg tcgaaaccgt caccagcctg gcggatccaa tcctggatag cgttggtgag
     1201 gtgcgggatg atctggacgt cgtcgccctc gaacttgccg gcgcgctcgt cctggataag
     1261 tttaagcaga atcttgccgc tcatgacgct gctggcgctg gtcagttcct cgtcgatgaa
     1321 gcgctcgtag tggccaaggt ccaggtcggt ctcggcaccg tctttcgtca cgaagcactc
     1381 gccgtgctcg cgcgggttca gcaagccggc atcgacgttc aggtacgggt cacatttttg
     1441 gaggttgacg gtaagcccgc gggctttcaa gaggttgccg atggaggcgg cggtgatgcc
     1501 ctttccgagc ccggaaagca cgccacctgt tacaaacaca tacttagtct ggcctttcgc
     1561 cacgcctgtt gcctcctcca gcaattcgta tctgttctat actacccgaa acttctaccg
     1621 cttggcaagg ctaaaccagg attaatgagc ggcaaggtat tgttcggcgg cttgcagctg
     1681 cgcgtcggtg ccaccgagcg tgtcgttcag gccggctttg acggcttggt cgggggtaat
     1741 gccatggtgg ttgatattct gtccgtccgg gcggtaccag ctggctaccg tcaccttcag
     1801 ttccgcgccg ccgccgaggt tgatcagctg ctggacgacg cccttgccga agctcttctg
     1861 ccccataacg taggcttcgt ggttgtcgtg caaggcgccg gtggtgattt cggacgccga
     1921 ggccgatccg ccgtcgatca gcacgaccgt cgggatgccg ccgagttcgt tgccgccggt
     1981 ggcgaaatgg tcgtcggtac cgatggtgcc gcgttcgctg acaatcagct tgccttcagg
     2041 cacccacaag ctggaaacct tcagcgccgc atcgagcagg ccgcccgggt cgccgcgcaa
     2101 gtcgaggacg atgcctctga cgccctgctg cttgaacttg tcggccgcct gctgcgccag
     2161 gttcgccgtg tcgtcactga acgtgctgat gtgcatatag ccgatgttgc cgtccaaaac
     2221 tttggtggtg acgcttggca cttggatatc ctgcctgata atactgagcg tcagggtctg
     2281 cgttttgtcg cggacgatac cgagcttcac ggtcgtgcca gccggaccgc ggattttgcc
     2341 gaccgcctcg tccaccgaca tgccggccgt cgactgaccg ttgatggtgg cgatgatatc
     2401 ttgcggcttc aggccggctt tatcggccgg gaaaccgctg atcggcgaaa tgacgataat
     2461 gttcttgtcg ctgtcctgcc ccagctcggc accgatgccg ctgaaggtat tgttcagctc
     2521 tccctggaac tgtttagcct cgctcggtgt aaagtacacg gtgtacgggt cgttggtggc
     2581 ctcggccagg ccatgcttca tgccgtttac gagctgctgc tcggtcagtt taccgttgta
     2641 attctctttc agcgcctggt aaacggtatt cacgctcgag taatccagct ggttcgggag
     2701 cccgttctga ccgctcttac cgaagtgaat ccggccgttg ccgatgttca cacccaggcc
     2761 gaagaccagg acgatgataa ctccgaaaag gacacccttc agcaatcctg tcagcccgct
     2821 tttgacgcgc accggctcgt caccctccac ctttttcttt cgcaatcgca ttagcagtaa
     2881 ttatagcaga cgaagcgtta gaccattcca aagcgacgtg ctaaggtatg tgattatcag
     2941 cccgtacggt gttcgggtac gcccctagga atcgaggagt actgccttca atcacgagct
     3001 cgtgcgcgct ggccgtcaaa gcagtctggt cttagccgcc gaagtagatg taggtcaggc
     3061 cggacccgtt gacccagtgg tcgtagaaat tgccgtcgta gccgtcgttg taggagtaca
     3121 cgtgcacctg atcaccgctg acacccacca cccacatggc atgaccatag gtaccgccgg
     3181 tgtagatagc aaccgaaccg accttaggtg tgtagcccgt agggatatga gctgcccggg
     3241 cactaccagg ccactggttg gcattgccaa agccgatggt attgatgccg taggtcttgt
     3301 acaccatcca ggcggtgtag ctgacgcact cgcggttaca cattccccag ctatccatgt
     3361 aggaacagcc cggcgtgtag tcactactgt gcacgtagcc gcagccccag ggaccgtacg
     3421 acccggaggc ggtctgcgga taactgccgc cgcatgaacc gctggtgatg accgcgccgg
     3481 aactgtccag ccgacggtta gcggccgcct gcgctgccct tagttgggct atcttgctct
     3541 ggttcgccga aatctgcgag ttgtagctgg cctgctggct ctcgttcatg ctgagcagct
     3601 gatcctgctg agccttggcg gccgacagct ggtcgttttc gttcttctgc gtagccagca
     3661 gctggtcgac ctgctccttt tggctctgga gcttcttctg caattcggta atctgctgca
     3721 cggtcgcctg gatcttatcc tggacgaccg agtaggcttc ctgcttgtcg atgtagtcgc
     3781 ttaaagagct gctggtggcc aacatctcga ccggcgtgat ttggccgttg atgtaggtgg
     3841 tcttcacgtc gttggccagc acggtcttct cgtagtcgat ctgcttctgg tcttcgacaa
     3901 tctgctgctg cagctgggcc tgtttggctt cgttgtcggc aatcgccgcc tggatggcgt
     3961 tgatctgcga ctgcagcttg tttatcgtgt cctgataact gctggcttgc gcctccaggt
     4021 tattcaaatt accctgggcg gcattgttct gctgctgcag ggcgttgatc tgggcatcgt
     4081 actggtcggc gcgcactagc ggcgtcgcca cggccatacc gaccgtcaaa agactcgcca
     4141 ctaccagaac cggcgtgctg cgcagccagg ccacgaattt acgactggtt ttcttttttt
     4201 caggcataat ttttgtttgg tttatgctac cggttattat acgcttcccc atctgtattt
     4261 gcaagttccc tcttatgctt tacggcgccg ttttccctcg gcacagagcg ggcgctaagc
     4321 tgcgatacga tgctcacgta cgggcgtacg ctgcgcttcg tttctcgctt atcatcccgc
     4381 tctgcactcg aggcgcaacc ggctacaggc ttgttttgcc tcgtgtttat aaattgttaa
     4441 ttggattttc ggatcttggt ttgctttctc ggctgttgtt ttttgtcgaa aatttcgggc
     4501 cggtcgggcc ggctaacttg cagtcaatca gtcgttcggg tcagtgctca tgctacttgg
     4561 tcttgaacct taggtaccgt ctggtggcga tgaaggaaga aacggccccg atgattatac
     4621 ccacggccaa ttgcagagtc aaaaataacc agaagtggga atcgaagtag gtcgtggcat
     4681 aaccgatgtc gagcaggccg aaggtggtcg cctggagcgt ggagctggag gcgacgaaga
     4741 cgccgttgat gataagcacc gaaatgacgg cagacagcat gccgtaaatg acgctctcga
     4801 cgatgaacgg accgcggatg tagctggtac tggcaccgag caagcgcatg atctggattt
     4861 cgtcgcggcg gttgaaaatg gccatctgga tggtgttgaa gatgatgagc gcgctgacca
     4921 cggcgaagac gatgacggcg atgatgccga tttcccgcag tacgttggtg gcgtgagtga
     4981 tgttgtcgat agcctgcttg aggttaccgg agtaagaagg cgggtcggat tccagcgccg
     5041 tgacgtccgg acgctccagg taggccttga tgtcattgag cttattgaga tcggtgggtt
     5101 taatgtggat ggtggccggc agcgggttgg ccgtttcggt cacggccgtc agcagctgct
     5161 ggttaccggc gttctggcgc tggtagttgg ccagcacctg cgccttgctc aggtatgtca
     5221 cgctctgggt gttgggcagc ttttccagct tggccaccag ctgtttggcc tggctttccg
     5281 tgacggagtc cttcaggtag accgacacgt cgatcttgct ggttatctgc gcgatggtat
     5341 tgctgaaggt ggcgttggcg ataagcgaga acagcacgat ggtcagcgtc acgatcatca
     5401 ccgccatggc cgcgatggcc aaggacgcgt tgcggatgaa gttcacgatg ccggtgtgaa
     5461 cgatgcgcat gaatgtggtg tatttacgtt gcatatgctg tggatttata tcttgtaact
     5521 ggcgttagcg cggtcgccga cgatgcgtcc gttctgaatg gtgacgacgc ggcgcttcag
     5581 cttgttgacg atttcgacgt tgtgcgtggt cagcagcacg gtggtgccgt acttgttgat
     5641 cttttccagc accttgatga cgtcccaggc gtgcttgggg tcgaggttac cggtcggctc
     5701 gtcggcgata aggatcttgg gctggcggac aatggcccgg gcgatagcca cgcgctggcg
     5761 ctcaccgccg gaaagttcct gcggcatgcg cttttctttg ccggtaaggt tgacgatgtc
     5821 gaggaccttc ggcacggtgt gcaggatctc tttgtggctg acaccgacga tctccagcgc
     5881 gaaggcgacg ttctcgaaca ccatcttgtt cggcaacagc ttgaagtcct gaaataccac
     5941 gccgattttg cggcgcagca gcgggatgtc tttgtccttc agtttgtcgt aatcgatgcc
     6001 gccgatgacg atcttgccac cgctcggccg ctcctcgcgc gtcagcagct tgagcagcgt
     6061 gctcttgccc gctcctgact gaccaacgac gataacgaac tccttcggct cgacatgcaa
     6121 agacacgcgg tccagcgccg gccccaggtt tttgttgtac gtcttggtta cccgatccaa
     6181 cagaatcatg aggctgcgcc tcctggcagc attttagtat ttgatttcgt cacaatcatt
     6241 agcgctattg tagcatattt gcttgtgcta aaagtacttt tatgctgccg ctacgctgat
     6301 ttgtccagag ccgcttaggt tataagaggg ctaccctccc cgttgcatga agcggttgga
     6361 cagccagagc cagcggacgg cgtattcgac cagctggcgc gccgtgagat gctcgctctt
     6421 ctccagctgc tcaaaaacgt ggcggaaatc atcgctgact ttgaactgca aggagacgtt
     6481 gcgtgtggcg ttggcatgtg ccggaacggg gtgctctatc aggttgcggt agttaggatt
     6541 actctcaacg agccccaaga acgtcatcag actacgtcgt accacttcgg ccatactgtg
     6601 cgacttaaca gtcgggatca ggtcatcgat gaagccgccg gtctgccccg agacgaccac
     6661 ccgcagcttg tcctgtccgt ctgaattagt cacaaaacca tttgctttgc tcatgggcct
     6721 attgtaatca acggtgtatg taaaagtcag aggactattt aactcagtta gcggcgatgt
     6781 tcgcttcgag gtaggcctca atgaaggggt cgagattgcc atccagaact tggtccgggt
     6841 cggtcgaagt gtagctggtg cgcaggtctt tgacctgttt gtacggatgc aggacgtagt
     6901 tgcggatctg gttgccccac gcggcttctt cgttgggtcc cttcagctcg ctgaccttct
     6961 cggcgtgctg ttcttgctgc agctgggcca gtttggagcg caggatggtc agggccagct
     7021 ctttgttctg cagctgggaa cgttcgtttt ggatggccac cacaatgccg gtcggcaggt
     7081 gcgtgacacg cacggcgctg tctgtcgtgt tgacggattg accgccgtgc ccgccgctgc
     7141 ggtaaacgtc gaccttcagg tctttatcgt cgatttccag ctcgtccggc gcatcgatct
     7201 ttggcgtcac ttccactttg gcgaagctgg tctcgcggct ttcggcgttg aacgggctca
     7261 ggcgcaccag ccggtgcacc ccgtgctcgc ctttgagctt gccgtaggcg aacgggccgg
     7321 ccagctccag cgtcacactt ttaagtcctg cttcttcacc cggcgattcg tcgatgagct
     7381 gcgtttgcca gccggctttt tcggcgtagc gctggtacat gcggaacagc atctgggccc
     7441 aatcctgggc gtcggtgccc cctgccccgg catggatgct gaggatggcg tcgtgatcgt
     7501 cgtagggtcc gttgagcttg agctgctctt tgtactcgtc gaattttttc gtaataaagt
     7561 ccagctgttg cgtgacctct tgctcgctgc cttcgacctt caggtggatg atgtcgataa
     7621 gctcatcggc ggcacccagc agctcgcgcc acggcttcgt tcgcgcttcc agcttggcaa
     7681 tctgcttcat cacggcttgg gctgccagat tatcctgcca gaaatccggt tgctgacttt
     7741 gggcttgcag tttatccagt tctttttcat gcgcgaacaa tttaagccgc tcggcggcat
     7801 cacggatatc cgattgcaac gctttggctt tagtcagtaa ttcgtccatc ggtaccattg
     7861 taaactcctc tgtaactctc tccaacttcg cacttcga
//
LOCUS       DASSFD010000076         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_9377, whole genome shotgun sequence.
ACCESSION   DASSFD010000076 DASSFD010000000
VERSION     DASSFD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9377"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="VFH39_01760"
     CDS             <1..1597
                     /locus_tag="VFH39_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924538.1"
                     /translation="VSPKGGRRKLLLFGGATVLVLAAMVGLVFGWYLPNTPQNVYNTG
                     LNRTGKAVSSVVDSVTDKQTLQAYQTSVISGSIKADVDGGSYGGDFTTSFDKADLTGG
                     LNVTLKDSGSSKTLSAKVMSHIPDGSVYPDLYFQLSGLKALGIDEFMPGASAYDGKWI
                     SVSSKYLQSIGDTYLKTSDNTKQQLTTDDIAEVARTAANTTQQYLFTTAPDKAVFEQR
                     SYVGKEKVDGISSYHYKVSLNVQHAKDYCVALGTAMLNTSAYKKLASDNSAQTAEKQS
                     LKSDCDRSVGDAVKPSDTYDMWIDGHYKLIHKIRVYDKTDHASYTDVGQTYKGGNKLS
                     LFVNVHDAKQQGDGAFTLDTDVSTRQTDATLSYKSTSADNPENVTVQLHAKLSADAVK
                     VTPPAGAVPIQDVLKQFGIDPTSSAGAASDTPVGLKAADTERETDIRSLQANLEAYYA
                     ENGAYPTLDLLNDASWQQTNLKGLDPSALVPPDSAAKTLAAAATKTQYGYAAADCKAQ
                     DCQAYTLTALLSNGSTFSVKSLN"
     gene            complement(1700..2257)
                     /locus_tag="VFH39_01765"
     CDS             complement(1700..2257)
                     /locus_tag="VFH39_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924539.1"
                     /translation="MVAYPHATTLVRFYRTLTSEGVEHVKLIRTASRLLGVLALAVVL
                     SLTDFVSHYAQATGSSTQHPQPATLTAKAEPVTTKIVINQPANGPLRETEFFKPFGHP
                     RTMVPHCPHSWCGGARFDRDPAQVVIIPAPKALFVSGEADYHCGPNGYLTIRRLSNGH
                     PTIPDSYGSDQGTCTWYHIVVRYHK"
BASE COUNT          534 a          672 c          736 g          419 t
ORIGIN      
        1 cgtctcgccg aaaggtggtc gccgcaagct gctgctgttc ggcggggcga cggtactggt
       61 gctggctgcc atggtgggcc tcgttttcgg atggtactta cccaatacgc cgcagaacgt
      121 ctataacacc ggcttgaacc gcactgggaa ggccgtaagc agcgtcgtcg acagcgtgac
      181 cgacaagcag acgctccaag cctatcagac gtcggtcatc tccggttcca tcaaggcaga
      241 tgtcgacggg ggcagctacg gcggcgactt cacgaccagt ttcgataaag ctgatttgac
      301 cggcggcctg aacgtcacgc tcaaagacag cggcagcagt aagacgctga gcgccaaggt
      361 catgagtcat atccccgacg gcagcgtcta cccggacctt tacttccagc tatccgggct
      421 taaagcgctc ggtatcgacg agttcatgcc gggggcgagc gcgtacgacg gtaagtggat
      481 ttcggtcagt tcgaagtacc tgcaatccat tggcgatacc tatctgaaga ccagtgacaa
      541 taccaagcag cagctgacga ccgacgatat cgccgaggtc gcccgcaccg ccgccaacac
      601 aactcagcag tacctgttca cgacggcacc ggacaaagcc gtgttcgagc agcgtagtta
      661 tgtcggcaaa gagaaggtcg acggcatcag tagctaccac tacaaggtca gcctgaacgt
      721 acagcatgcg aaggactatt gcgtggcgct aggcacggcc atgctgaaca ccagcgccta
      781 caagaagctg gcgagcgaca acagcgcgca gaccgccgaa aagcaaagcc tgaagagcga
      841 ttgcgaccgg tccgtcggtg atgccgtgaa accatccgac acctacgata tgtggatcga
      901 cgggcactac aaactcatcc ataagatccg cgtgtacgac aagacggacc acgccagcta
      961 tacggatgtc ggccagacct acaagggcgg caataagctg tctttgttcg tgaacgtgca
     1021 tgatgccaag cagcagggcg acggcgcctt tacgctggac accgacgtct cgacacgcca
     1081 gaccgacgcc acgctgagct acaagagcac aagcgctgac aatcccgaga atgtgacggt
     1141 gcagttgcac gccaagctgt cggcggacgc cgtgaaggtc acgcctccgg ccggtgcggt
     1201 gcccatccag gatgtgctga agcagttcgg catcgatccc accagcagcg ccggtgcggc
     1261 cagcgacacg cccgtcggcc tgaaggcagc ggataccgaa cgcgagacgg atattcggtc
     1321 gctgcaagcc aacctggaag cgtactacgc cgagaacgga gcgtatccaa cattggatct
     1381 tttgaatgat gcatcgtggc aacagacgaa cttaaaaggc cttgacccat cggctctggt
     1441 cccaccggat agcgctgcca aaaccctggc ggcagctgcc accaagacgc agtacggtta
     1501 tgcggcggct gattgcaaag ctcaagattg ccaagcctat acgctcacag cgctcctcag
     1561 caatggcagt accttctcgg taaaaagcct caattaatac gtttggttca taagtaagtc
     1621 atcggaaggc ggagctgcgg gtggtgctgc cgaggggcag gtcctggtaa ggtcccgccc
     1681 ctcggctggc gcacctgggc tacttgtggt agcgcaccac gatgtggtac cacgtgcaag
     1741 tgccctgatc cgatccgtag ctgtccggaa tggtcgggtg accgttactg aggcgacgga
     1801 tagtcaggta gccgttcggg ccgcagtggt agtcggcctc gccgctgacg aacagcgcct
     1861 tgggcgccgg gatgatgacg acctgggccg ggtcacggtc gaagcgggca ccgccgcacc
     1921 agctgtgcgg gcaatggggc accatcgtgc gcggatgacc gaacggtttg aagaactccg
     1981 tctcgcgcag cgggccgttg gcaggctggt tgatgacgat tttcgtggtg acgggttcgg
     2041 ccttggcggt cagcgtggcg ggttgcgggt gctgcgtgct tgaaccggtc gcctgcgcgt
     2101 agtgcgacac gaagtcggtc agcgacagga cgacagccag agcgaggact cccaggaggc
     2161 gggaggccgt acggatgagc ttcacgtgct caacaccttc cgatgttagg gtccggtaga
     2221 accggacgag cgtggtggcg tgggggtacg cgaccatcaa tagtatatta tagcaaaaaa
     2281 taaagctaat gtcaatttac ggatggttga ataacagggg tgaatgccgt aggcgcagcg
     2341 gtctgctatc atgaacctat g
//
LOCUS       DASSFD010000077         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1775705, whole genome shotgun sequence.
ACCESSION   DASSFD010000077 DASSFD010000000
VERSION     DASSFD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775705"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..775)
                     /locus_tag="VFH39_01770"
     CDS             complement(158..775)
                     /locus_tag="VFH39_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924540.1"
                     /translation="MPKSAEPSFKHVQVWLEQQPPHPLLASAERRQSFPVEGLNYLMD
                     EEVPLRWQLFQKAREAGDDTEAAVHLLSAMYAAHTAVLYHDAGHGVPIAAGRQSITKL
                     MTDWKVNTPRDRLQATKQRWALDFDKVMGKPYLANKAGACLMPGFQTLVNSAVMLDLE
                     TGCLAEPPKSEPTVEERTAPEPHRISRRRRALVTAKYFANSFFGS"
     gene            806..1594
                     /locus_tag="VFH39_01775"
     CDS             806..1594
                     /locus_tag="VFH39_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924541.1"
                     /translation="MQADYWHRQLAGKPLFPELEWSRPENRNQAGKLLIIGGNAHGFA
                     APGEAYGEATKAGIGSTRVVLPQRVKSLVGSVLPDVDYAMSNPSGGFSQLALNDLLMW
                     ADWADGVLLAGDFGRNSETAILLEKFLAHHAGQVTLTKDAVDYFTPSPQALLARDDTT
                     LALSFAQLQKLAMNGRFTTAFTSDMDLIRCVDALHEFTTLHSVNLITKHLENLIVAVD
                     GQVSTTKLEEVKIWRLKTAAHASVWWLQTPAKPFEALTTSLVTK"
     gene            complement(1784..1868)
                     /locus_tag="VFH39_01780"
     tRNA            complement(1784..1868)
                     /locus_tag="VFH39_01780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1832..1834),aa:Leu,seq:caa)
BASE COUNT          478 a          621 c          575 g          431 t
ORIGIN      
        1 cgccggtgat gccgccatgc tcgagcttgt cgagcggccc gtcgccttca acccgaaccg
       61 tgaactgttc gacatcgcta cggagcatac ctggccgatt gtagtcgaac gcaagaacgt
      121 catttactgt ctggagggcc gcgacgggcg ttactgctta gctaccgaag aatgagttcg
      181 cgaaatattt ggcggttacg agagcccgtc ggcggcggct aatcctgtgc ggttcaggcg
      241 ctgtccgctc ttcgacagtc ggttcagatt ttggtggctc tgccagacag cccgtctcga
      301 gatcaagcat caccgcgctg ttaaccaacg tttgaaatcc aggcatcaag caggcccccg
      361 ccttattagc caggtatggt ttacccatga ccttatcgaa atccagagcc catcgttgct
      421 tcgtggcctg caaccgatcg cggggtgtgt tcactttcca atcagtcata agttttgtga
      481 ttgattgtct gccggcagca atcggcacac catgtccagc atcgtggtag agcacagcgg
      541 tatgagcagc atacattgcg ctgagcagat ggacggcggc ttcagtatca tcccccgcct
      601 cgcgggcttt ctgaaacaac tgccacctga gcggcacttc ctcgtccatg agataattca
      661 ggccctcaac cgggaaagac tggcgtctct cagcgctggc aagcaatgga tgtggcggct
      721 gttgttccaa ccacacctgc acatgtttga acgaaggctc cgcacttttt ggcatattgg
      781 cgctatcata gcataatttg taatcatgca agcagactat tggcaccggc agcttgccgg
      841 caaaccgctc tttccggaac tcgaatggag ccggcccgaa aaccgtaacc aggccggtaa
      901 actgctgata atcggcggca acgcccatgg gttcgccgca ccgggcgaag cctacggaga
      961 agccacgaag gccggtatcg ggtcaacccg ggtcgtgttg ccacagcgcg tcaaaagcct
     1021 ggtcggcagc gtcttaccgg atgtggatta cgccatgagc aacccgtccg gcggtttcag
     1081 ccagctggcg ttgaacgatc tgttgatgtg ggccgactgg gccgacgggg tgctgctggc
     1141 cggcgatttc ggccgcaaca gcgagaccgc catattgctc gagaaattcc tggcccacca
     1201 cgccgggcaa gtaaccctca ccaaggacgc cgtcgattac ttcaccccat cgccacaggc
     1261 cttgctggca cgggacgaca cgacgctggc cctgagcttc gctcaactgc agaaactggc
     1321 catgaacggg cgctttacca ccgccttcac ctccgatatg gacctcatcc gctgcgttga
     1381 tgccctgcac gaattcacga cgctccatag cgtcaacctc ataaccaaac acctcgaaaa
     1441 cctcatcgtc gccgtcgacg ggcaggtgag cacgacgaaa ttagaagaag tgaagatctg
     1501 gcgcctaaaa acagcggccc acgccagcgt ctggtggcta cagaccccag ccaaaccgtt
     1561 tgaagccctt acgacaagtt tggtaacaaa ataaaaaacc ggctatgccg gttcaaaaat
     1621 tggtgggcga ggagggaata tcacagatat atctgcgtcg cgtgctcgga atgcattcct
     1681 gcgcgcgctc cttgctatcg aaccctcgcc agcatggctg gcttccgccg ggttcgcttc
     1741 tgtcaacaac caatttttag accggctatg ccggttcaaa aattggtggg cgaggaggga
     1801 atcgaaccct caatccgtaa ggaacacgat tttgagtcgt gcgcgtatac caattccgcc
     1861 actcgcccgg ataaaccggg tgcgaaggcc aggcgacaaa ccggctgtaa aagcaggtgc
     1921 gcgtaggcta tcaggggtgt attgtactat actattggca agagacaaac tccaaggacc
     1981 ctcatattat gaatctgcaa ccgctcaatg accaaccgga cgagcgcccg caagcgggcg
     2041 ctgattcgtc tacggctgct gatttgatcc gccagaaagt ggctcggctg tacgccgaag
     2101 agccg
//
LOCUS       DASSFD010000078         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1748658, whole genome shotgun sequence.
ACCESSION   DASSFD010000078 DASSFD010000000
VERSION     DASSFD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748658"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1021
                     /locus_tag="VFH39_01785"
     CDS             230..1021
                     /locus_tag="VFH39_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924542.1"
                     /translation="MSTELSLQPPVYLELVDYAVPQESRAEVVLDAFKEVSQQPDATP
                     QTQEAAGFLLGLYAVERGGQLRAGEPVRPEDLRHFGVELSAPELHRFIGVLNSVVERT
                     ILASPHSFLGQAALRKYKNQLRSEMLAARTAAEPTEPEMPTATAIDHQRANAIQRGLL
                     SQRCLDEGIEDSQGHMVRQIREGQEAERTLFAVKKKLLDPMLEQYEDDTVPQDVLRKV
                     AEASLQEAVRAWRPVEGSKDTFETFAKLRIGPVLHRYYNEQALLG"
     gene            complement(1038..>3466)
                     /locus_tag="VFH39_01790"
     CDS             complement(1038..>3466)
                     /locus_tag="VFH39_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET6924543.1"
                     /translation="AAELVPGDIVYIEEGDSVPADLRVMDEEELASNDFALTGESNPS
                     RKFVHAISGDVPLANRHNLLFMGTTVATGHGHGVVVGTGMHTELGRIASLSQVVSTDS
                     SPLQKEMNHLAKRLVQGTVVLAVLLVGVALKTKFGLHDALLFGIGIAAAMIPNGLAAE
                     VNITLAQTANRMAKARALVKKLSAVETLGATNIILTDKTGTLTKNEMTVTRLLIGKTE
                     YGVTGTGYEANGTVIDSKGKPLGKAKLKELQLFFETAAMASNARVHAPDTQHAVWYVV
                     GDPTEGALITLAQKAGINPDGLDEQHAEIKEFQFDSARKLMSSVRRMADGRILVFAKG
                     APESILSRSSTLHDHTFTRRLTAADRKRLLAYNSELAGQALRNLAMAYHVLPAKTDLN
                     NLKMDGVEQGFTFLGMASMLDPLRDAVPKAMIAARHAHIRVSIVTGDNPTTAQAIARH
                     AELATSPDDVVIVTGDELHKLADSQVLELVVQGGAVFSRVAPEDKLRIVEIAKQGGRV
                     VAVTGDGINDAPALKRADIGVAMGKTGTDVAKDAAEIVLLDDSFSTLVSAVEQGRLTF
                     QNIHKAARCALTDNAGELITILISLVGQAVFHVPLAITAVQILAIDIIAQMFPITALG
                     WDKAQASLLHQPPRKLSDHIINRHAVMEFVGFGFLSAALAYLNFLFFFVRRGLSPVNI
                     DPNSTLYFKATILTYVTIVLCQFLNLLLVRSDEHEKFFSRYLWSNRKLLVAFGISFFC
                     IANIMYNPWIRPYFHAGPLTWGDWLTAVLTAVIYISFRLFQRHSRRHSRKAILRDHHP
                     DHIRKHLRTA"
BASE COUNT          671 a          959 c         1125 g          711 t
ORIGIN      
        1 gccaccggcc atgccggcaa caccgccgat agctccccgg caggccgccc ccccgatagc
       61 tggtgctccc tccgtcaccc cagttccggc gccgccgcag ccggacctca cggttgatca
      121 tcccatacaa cctccctcca agccggaatc tccacagtca ccaacagctt aagttagcgc
      181 ccaccgtacc gtatttgccg caagtaagca tatgtggtat aatataggta tgtctacgga
      241 actcagctta cagccaccgg tctacttgga attagtcgac tatgccgtgc cacaggagtc
      301 gcgggcagaa gtagtattgg atgccttcaa ggaagttagc cagcagccgg atgctacacc
      361 ccagacgcag gaagccgccg gtttcttgct gggtctgtac gccgttgagc ggggcggcca
      421 gctgcgtgcc ggagagccgg tccggccgga agatctgcgc cattttggcg tggaactatc
      481 agccccggaa ctacaccgtt tcatcggggt gcttaacagt gttgtcgagc gtactatctt
      541 agctagccct cattctttct taggccaggc ggcactgcgc aaatataaga atcagctgcg
      601 gtcggagatg ctggcagcga gaacggcagc tgaaccaacc gagccagaga tgccaaccgc
      661 gaccgctatc gatcatcaac gggcaaacgc catccagcgg ggtttattga gccagcgttg
      721 tcttgacgaa ggaatagaag attctcaggg gcatatggtg cgccagatac gagaaggcca
      781 ggaagctgag cgcaccttgt ttgctgtcaa gaagaagctg cttgacccta tgctggaaca
      841 gtacgaggac gatacggtcc cgcaggatgt gcttcgaaaa gtggccgaag cgtccctgca
      901 agaagcagtc agagcgtggc gaccggttga aggctccaag gatacctttg agacgttcgc
      961 gaagctgcgc atcgggccgg tactgcaccg gtattacaac gagcaggcct tacttggctg
     1021 acggttcatg acttccctca ggcggtacgc agatgtttgc ggatgtggtc ggggtgatgg
     1081 tcacgcagga tggctttgcg gctgtggcgg cggctgtggc gctggaacag acggaacgag
     1141 atatatataa cagcggtgag gacggctgtt aaccaatcgc cccaggtgag cggcccggcg
     1201 tggaaatacg gccggatcca cgggttatac atgatattgg caatgcagaa gaacgagata
     1261 ccgaaggcga cgagcagctt gcgattgctc cacaggtagc ggctgaagaa cttttcgtgc
     1321 tcatcgctgc gcaccagcag caggttcagg aactgacaga ggacgatggt gacgtaggtg
     1381 aggatggtgg ccttgaaata cagggtggag ttcgggtcga tgttgaccgg gcttaggccg
     1441 cggcggacaa agaagaacaa gaagttgagg taggctagag cggcgctcaa gaaaccgaag
     1501 cctacgaatt ccatgacggc gtgtcggttg atgatgtggt cgctgagctt gcgcggcggt
     1561 tggtgcaaca agctggcttg ggccttgtcc cagccgaggg cggttatagg gaacatctgg
     1621 gcgatgatgt cgatggccag gatctgaacg gcggtgatgg ccagcggtac gtggaagacc
     1681 gcctggccga ccaggcttat taggatggtg atgagctcgc cggcgttgtc ggtgagggcg
     1741 cagcgagcgg ctttgtggat gttctggaag gtgagccggc cctgctcgac ggcgctgacc
     1801 aaggtgctga agctgtcgtc cagcagcacg atttcggcgg cgtctttggc gacatcggtg
     1861 ccggtcttgc ccatggcgac gccgatgtcg gcccgcttga gggcaggggc gtcgttgatg
     1921 ccatcgccgg tgacggccac cacgcggcca ccctgtttgg caatctcgac gatacgcagt
     1981 ttgtcttccg gagccacgcg actgaagacg gcgccacctt ggaccaccag ttccagtacc
     2041 tggctatcgg cgagtttatg cagctcgtcg ccggtgacga ttacgacgtc atccgggctg
     2101 gtggccagtt cggcatgccg ggcgatggcc tgggcggtgg tagggttatc gccggtgacg
     2161 atagaaacgc ggatgtgggc gtgacgggcg gcaatcatcg ccttgggcac ggcgtcgcgc
     2221 agcgggtcga gcatgctggc cattcccagg aaggtgaagc cttgctctac cccgtccatt
     2281 ttcagattat tcaggtcggt tttggccggc agcacgtggt aagccatagc gagattgcgc
     2341 agggcttggc cagccagctc gctattgtat gcaagcagcc gcttgcggtc agcagcagtc
     2401 aggcggcgag taaaagtgtg gtcatggagc gtggagctgc ggctcaggat tgattccggg
     2461 gcgcccttgg caaacaccag gatgcgacca tcggccatgc ggcgtaccga gctcatcagt
     2521 ttgcgggcgg agtcgaactg gaactctttg atttccgcgt gttgttcatc caagccgtcc
     2581 gggtttatgc cggctttttg cgcgagcgtt atgagggcgc cttcggtcgg atcaccgacc
     2641 acataccaga cggcgtgctg ggtatcgggc gcatgcacgc gggcgttgct ggccatggca
     2701 gcggtctcga agaacagctg cagctccttc agtttggcct tacccagcgg cttgcccttg
     2761 ctgtctatca ccgtgccgtt ggcctcataa ccggtgccgg ttacgccgta ttcggtcttg
     2821 ccgatcagca ggcgtgtgac cgtcatttcg ttcttggtga gagtgccggt tttgtcggtc
     2881 aggatgatgt tggtcgcgcc caatgtttcg acagctgaca attttttgac cagcgctcgg
     2941 gctttggcca tccggttggc ggtctgcgca agcgtaatgt tcacttcggc ggccaaaccg
     3001 ttggggatca tggcggcggc gatgccgatg ccgaacagca gcgcgtcgtg caggccgaac
     3061 ttcgtcttta atgccacgcc gaccagcagg acagccagca ccacggtgcc ctggaccagc
     3121 cgcttggcga ggtggttcat ctctttctgc aggggcgaac tatcggtact gaccacctgg
     3181 cttaagctgg ctatgcgtcc cagctcggtg tgcatgccgg tgcccaccac gacgccgtgg
     3241 ccatgaccgg tcgccacggt ggtgcccatg aagagcaggt tgtggcggtt agccagcggc
     3301 acatcgccgc tgatggcatg gacgaacttg cggctggggt tgctctcgcc ggtgagggcg
     3361 aagtcattgg aggccagctc ttcctcatcc ataacccgca ggtcggctgg cacggaatca
     3421 ccctcttcta tatagacgat gtcgcccggc accagctcgg cggcat
//
LOCUS       DASSFD010000079         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_526517, whole genome shotgun sequence.
ACCESSION   DASSFD010000079 DASSFD010000000
VERSION     DASSFD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526517"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..280)
                     /locus_tag="VFH39_01795"
     tRNA            complement(206..280)
                     /locus_tag="VFH39_01795"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Met,seq:cat)
     gene            complement(345..806)
                     /locus_tag="VFH39_01800"
     CDS             complement(345..806)
                     /locus_tag="VFH39_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET6924544.1"
                     /translation="MQNKIEQYQELLWQFLRCLQDVKFAGQVVFVIIVLLISWSGIKA
                     IQTNYDLQKQITAQKQQNQLQSLRNNNLALENEYYNTDTYLDLAARQNFGLAAPGEKE
                     VLVPVDVARSYTVPLPAQPTTTDQPTDKQPVWQRNYQAWVNFFMHREQPTG"
     gene            1080..1155
                     /locus_tag="VFH39_01805"
     tRNA            1080..1155
                     /locus_tag="VFH39_01805"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1113..1115,aa:Ile,seq:cat)
     gene            complement(1239..1388)
                     /locus_tag="VFH39_01810"
     CDS             complement(1239..1388)
                     /locus_tag="VFH39_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924545.1"
                     /translation="MAQDDDMDMDRDNDMDLNDPDMMDDPTREDLTKSMGDEQDEEAD
                     DMKSM"
     gene            complement(1457..2290)
                     /locus_tag="VFH39_01815"
     CDS             complement(1457..2290)
                     /locus_tag="VFH39_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924546.1"
                     /translation="MLLSLLSKFLASLLVASITLVTLVVLFSQTLLSSHYIEGQLSAT
                     HSYSRLSVALSDELIQQASDQGAQVDPSLKQELQGVLSPVVMQQKLSLALEELQTYYQ
                     GKGPAPQINVSDLVARARAVGVQVPPDSNLDQPITFAAGRGVKGAGKTYAHVKWLLLA
                     AAAVLAGLLLLVCWKRRRWVALPDVFIGTGVLLGLSALVFGFGAGIAERYVSFGLASN
                     AFTAIGHDLAVAISHDLGRRLVIIAICYLVVGVALRIMVAHHHPKTRSVPAKAPQRPN
                     L"
BASE COUNT          509 a          635 c          617 g          543 t
ORIGIN      
        1 attgaatatt gagaatcttc cgatacagcc ttgagcggct gtatatctac ttttccagta
       61 aaccattctg ccggaccttg gtttgcatct aaatggggta atatttgcat agcttaatca
      121 tatcacttct gttttgagga gtaaggttcc aacacaccaa aaatggtccc atttttggaa
      181 ccatttctta tctggcaatt tgacttggtt gcgggggcag gactcgaacc tgcgacctca
      241 tggttatgag ccatgcgagc tgccgctgct ccaccccgcg tcatttggcc gcgattttcg
      301 cggctacttg ttataagtat agcaacagag gtagctaaag tcaatcagcc ggtcggctgc
      361 tcgcggtgca tgaagaagtt cacccacgcc tggtagttgc gttgccacac aggctgcttg
      421 tcggtcggct gatcggtcgt ggtcggctgt gccggcagtg gtacggtgta actgcgggcc
      481 acatctaccg gcaccagcac ttccttctcg ccgggcgctg ccaggccgaa gttctgccgg
      541 gccgccagat ccaggtaggt atcggtgtta tagtactcgt tctccagcgc caagttgtta
      601 ttgcgcagcg attgcaactg gttctgttgc ttctgggcag taatctgctt ttgcaggtcg
      661 tagttggtct ggatggcttt tataccgctc cagcttataa gcaagacgat gatcacaaac
      721 accacctggc cggcgaattt cacatcttgc aggcagcgaa ggaattgcca cagcagctcc
      781 tgatattgct caattttgtt ttgcatacgt cgctaccagt atataactat ccctaaaatt
      841 ggtatatttt ccggtcagga aatatatgcc gagccctgga atgcaagcca gtaggatatt
      901 ggcattcacg gcgcgaagct atatactctc atctgttagc aatgcagcta aaatcaaaat
      961 cgtaatccaa ggagccgcta ggcgactgaa tgcgattttg agaggaacag cggagacgta
     1021 agccgaagct tttgccgaac tttcggcaaa acgaggcagc gacgtaggct gcgacgaagg
     1081 ggggcgtagc tcagtggtta gagcagtcgg ctcataactg attggtcgtt ggttcgaacc
     1141 caaccgcccc caccaatcca tgcacaagcg aaaactcccg cctatcttgg cgggagtttt
     1201 catagacggg gattactcga gcgaacgtag cctggcggct acatggattt catgtcgtcc
     1261 gcttcttcgt cctgctcgtc gcccatggac ttggtcaggt cttcgcgggt cgggtcgtcc
     1321 atcatgtcgg ggtcgttcag gtccatgtcg ttatcgcggt ccatgtccat atcatcgtct
     1381 tgtgccataa caatgcctcc tttcgttagt aggcctcact atacggtggc tggctacgat
     1441 gcttcagcta gaattcttac aggttgggcc gctggggcgc cttggcaggg acagagcggg
     1501 ttttaggatg atggtgcgcc accataatac gaagggcgac gccaacgact aggtagcaga
     1561 tggcaattat taccagccgc cttccaaggt cgtgcgaaat agccacggcc aagtcgtgcc
     1621 cgatggccgt gaacgcgttc gatgccaggc cgaacgaaac gtaccgctcg gctatacccg
     1681 ctccgaatcc gaacaccagc gccgatagac ccagcagcac acccgtaccg ataaacacgt
     1741 ccggcaaagc cacccaacgg cggcgcttcc agcataccaa cagcaacagg ccagccagca
     1801 cggcagccgc ggccagcagc agccacttca cgtgggcata ggtcttgccg gctcccttca
     1861 caccccggcc ggcggcaaag gtgatcggct ggtccagatt gctgtcgggc ggcacctgca
     1921 cccctacggc gcgggcccgc gctaccaggt cactgacatt tatttgcggt gccggcccct
     1981 ttccctggta gtaagtctgc agctcttcca gcgccaggct caatttctgt tgcatgacca
     2041 ctggcgagag cactccctgc agttcctgtt tcaacgaagg atcgacctgg gcaccttgat
     2101 cggatgcctg ctgaatgagt tcatcgctca atgccactga taggcggcta taactgtgcg
     2161 ttgccgacag ctgaccttct atgtaatggc tgctgagcag cgtctggctg aagagcacta
     2221 ccagcgtcac caatgttatg gaggctacca gcagcgaagc cagaaattta gagagtaacg
     2281 acaaaagcat gagcagcatt catt
//
LOCUS       DASSFD010000080         9564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1811620, whole genome shotgun sequence.
ACCESSION   DASSFD010000080 DASSFD010000000
VERSION     DASSFD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811620"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..358)
                     /locus_tag="VFH39_01820"
     CDS             complement(116..358)
                     /locus_tag="VFH39_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924547.1"
                     /translation="MSYRWAPLGVRGRHDCIGADRSGLPARSQEQLRADLEARKALVV
                     AEAAGEQAVRQQTELVLPAEEVRVFDLTSVEVGANA"
     gene            626..2911
                     /gene="topA"
                     /locus_tag="VFH39_01825"
     CDS             626..2911
                     /gene="topA"
                     /locus_tag="VFH39_01825"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET6924548.1"
                     /translation="MAKNLVIVESPAKAKTIGQFLGKDYTVKSSFGHIRDLPKKGLNI
                     DIKNNFAPTYALNTDKRKVMSELKTAAKGAEVWLASDEDREGEAIAWHLTQALKLDPA
                     KTKRIVFHEITKPAIEAAIKNPRTVDLHLVDAYQARRVLDRLVGYELSPVLWKKVRTG
                     LSAGRVQSVAVRIIVDREREIRAFEPESSFKVTAQFSVHGESFPAELTNRLDSAEGAE
                     QWLQSVQDAVFTVGNVATKPGSRSPGAPFTTSTLQQEASRRLGYSVRQTMTIAQRLYE
                     AGHITYMRTDSTTLSGQAIGAAKDYITATYGANYHQVRQYKTKSQNAQEAHEAIRPTD
                     FKRLEAGEDSQQAKLYQLIWQRAIASQMSSAELERTEVTIDISGHQEQFLAKGEILRF
                     DGFMKVYGGGKDDTLLPDLKTGDDLSAESITAVETFSRPPARFSEASLVRKLEELGIG
                     RPSTYASTISTIQSRGYIEKRDLDGVERHVREITLSGGEVTPSSRSEITGADRGKLVP
                     TPIAEVTTDFLVKYFPAIVDYDFTATVEEEFDEVARGEAEWHQMIADFYKDFHSLVLE
                     SADVSREEVSQARLVGNDPKSGKPILARFGRYGPMLQRGETESEEKPDFAPLPKDTTI
                     DTVTLEQALTAFQLPRVVGTTEDGQEIKANVGRFGPYIQIGKLYVSIKDHDPHTIDEP
                     TSRELYKAKLAAEAAKNIQNFGAIKVLNGPYGPYVTDGKKNARIAKDVDPAKLTEAEA
                     EALLKAAPAKKRFTRRRTAKK"
     gene            3141..4187
                     /locus_tag="VFH39_01830"
     CDS             3141..4187
                     /locus_tag="VFH39_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET6924549.1"
                     /translation="MAETTTTAHSLNTEQLVNDTLATIDREREREIVARRFGLFERKE
                     TLEQIGELLGITRERVRQLEKAVVTRLKTAAEQGHLQHIPEFQQTVSRELEKVGGIAR
                     ISELSNTLGDSGRLEQARLAFLSGLCPELIVIDDDDNYYHSVGIRKSFDIASAKGQVE
                     KLIAVIKKLGEPKPIEDVASAAGMSDVKQAAALAGISKQLATLNGRWGLVKWPMVNPK
                     NIRDKIYVILKENGKHMHFNEIADAIKKSDFRRKDVTTQAIHNELIKDKRFVLIGRGI
                     YALKEWGYEKGTVADIITEVLQKAGEPLHRDEIVKRVLKSRFVKETTILLNLQGKPQF
                     KRVAKATYTLAEEE"
     gene            4199..4777
                     /locus_tag="VFH39_01835"
     CDS             4199..4777
                     /locus_tag="VFH39_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924550.1"
                     /translation="MAAPEVLNVTRPDFDPLEHLAPFARHQNIAFHPAKPGKILRWYP
                     HQDTWDCFDSPKGANSALHMNQQHLGFLASATRIAVPKHYDFIAQEPPYSNENPAYAV
                     FSELDLIAGCRPVDAKDDVRLVWEALTKYHSWVVTTDQPVILHDAFKLDQYHVTNKGV
                     TLLDIDPYFAETRGHTMGLVRQQLRNIGTIVS"
     gene            complement(5508..6431)
                     /locus_tag="VFH39_01840"
     CDS             complement(5508..6431)
                     /locus_tag="VFH39_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924551.1"
                     /translation="MKLFRAKATPPPPRRVTRSARPQAFSYHANRVEQDYNIGRSQPR
                     DQDVRRHEQFVRFWRQRLSVLFMGALILICVGYILHLSPTPQIVPLTSNSADYVLQPA
                     SVYQTAASRALRSSWLNANKITVDTTRLSHSLKQQFPELAAVSVTLPLMGHRPIIYVS
                     PKQPALILQEADTSYVIDTTGQVLDRATALGPTSRQQLPTVIDKSGLKLQPRADALPA
                     SSVTFISYVAQELRAKAVSIQSVTLPEKAYELDVRVAGKPYYVKFNLHDYQHAQAQVG
                     TMLAVMKRLQEQGTTPSQYIDVRLAGRAYYK"
     gene            complement(6439..7560)
                     /locus_tag="VFH39_01845"
     CDS             complement(6439..7560)
                     /locus_tag="VFH39_01845"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6924552.1"
                     /translation="MAVASSLKQLDPGLRIVYIGQAGDKLADIPAADPNVDEVYTVRA
                     GKFRRYHGEGWRQLLDLPTQFKNIRDAVYVLIGLVQSYRLMRRLKPSVVFSRGGFVSV
                     PVALGGHLAGVPYITHDSDSVPSLANRLIARWARLHAVALPEDSYPYPRNKTKMVGIP
                     LLADYQPVTAATQAAYKHQVGVAKDGRLLLVTGGGNGAQELNEAVVANAPFLLKRYPD
                     LHIIHVAGRDHEAAIGQAYDTALTAEERQRVLVHGFVTEFYRYSGAADIIIGRAGATN
                     LAEFAAQGKVCVIVPAGQLVGGHQLKNARILAREQAIVLMTSEQIEQERRLTHVISEL
                     LDDATKRRELADHIARFARPNAAEELAALILEIAGGPQV"
     gene            complement(7547..8815)
                     /gene="ftsW"
                     /locus_tag="VFH39_01850"
     CDS             complement(7547..8815)
                     /gene="ftsW"
                     /locus_tag="VFH39_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET6924553.1"
                     /translation="MSWQPGQGERRPGGQAFRRAEPVSELGRRHKPDYWLVALCVILL
                     CIGLIVVYAISPALSASAHVSSNYYVGKQLIAIGLSAVAFVVTSQVPLSWWRQAYKPL
                     LVIAALATLVAIVMPVNPNYPAHRWVRLGSFSFQSVELLKFALLIWLSGFLSFRMRSG
                     KMADFNATLKPLLIALGIIGVMIAGVQSDLGSTGVIVLMMGVMAFVAGLPMKRVLAIA
                     GIVAIGLILAISSTPYRRERLMTYLHPEADCQTSGYQECQALIAVGSGGMIGLGLGRS
                     VQAYGYLPEAANDSIFAIYAEKFGFIGVLILLAIFIAFFHRLKSIAERSPDDFSRLLV
                     VGVLTWLSIQALINIGAMIGLLPLKGITLPFISYGGTSVVFTAAAVGLVFQISRYTSY
                     VTLTNDRSGNDYSRDRRRVRGAYHPNFGGR"
     gene            complement(8812..>9564)
                     /locus_tag="VFH39_01855"
     CDS             complement(8812..>9564)
                     /locus_tag="VFH39_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924554.1"
                     /translation="ETWLPLAGMLAAGLIGLVDDVMNIRGVNTSIAGMKSGIKFALYS
                     LVALVGGWWFYAKLGVNTLYLPGIHNWHIGIAVIAVFWLVVVATANSVNISDGLDGLA
                     GGLLASAFGAYALIAGIENKYALAGFCLTVVGALLSYTWFNIYPARFFMGDVGSFALG
                     TALGIIAMQTNTVYVLPIIGFVFVMETGSVIINRLSRKLRHGKKVFLSSPIHHHFEAM
                     GWPETKVTMRFWILGQIGAVLGLALFVLGRYR"
BASE COUNT         2215 a         2801 c         2668 g         1880 t
ORIGIN      
        1 gtgctgtcgg gacatgaaat agttttgcca ttgttgcctc tgtctgtacg caagtacgct
       61 cccctctgtg gggagcgtag ccctacgccg attctcttaa tcctctaacg cctggttacg
      121 cgttcgcgcc gacctcaaca gaggtcaaat cgaacacccg cacctcttct gccggcagga
      181 ccagctcggt ctgctgcctt acggcctgct cgccagcagc ttcagcgact actagtgcct
      241 tgcgtgcctc caagtcagca cgcagctgct cttggctacg agccggcaaa ccgctcctgt
      301 ccgcaccgat gcagtcgtga cggcctcgca cgcctagggg ggcccaacga taactcatat
      361 attcttattt ccttccaggc acattttaag agagccgcta gcgcgcggca ccccagtttt
      421 tgtgcccact taatttattc cgtattccgt tttacgcttg tcagtgatgg gtgtcaagca
      481 ttttgtgtga gatttctcac acattatccg gcgcttgcat ttgaacttta aaacctgtaa
      541 gctgactcac cgtgtccatc gtcactataa atagtagagt aatggtggaa aatcataagc
      601 gtacaggtat actaatagga cattcatggc aaagaacctc gttatagtag agtccccggc
      661 aaaggctaag accatcggcc agttcctggg caaggattac acggtcaaga gcagctttgg
      721 ccatatccgt gatttgccga aaaaaggcct gaacatcgat attaaaaaca actttgcgcc
      781 tacctacgcc cttaacactg acaagcgcaa agtgatgagc gagctgaaga ccgccgccaa
      841 aggtgccgaa gtctggctgg cttccgatga ggaccgcgaa ggtgaggcca tcgcctggca
      901 tctgacccag gcgctgaagc tggaccccgc caagacgaag cgcatcgtct tccacgagat
      961 taccaagccg gccatcgaag ctgctataaa gaacccccgc accgtggacc tgcacctggt
     1021 agacgcctac caggcccgcc gtgtcctcga ccgcctggtc ggctacgaac tgagcccggt
     1081 cctctggaag aaggtccgga ccggcctgag cgccggacgg gtgcaatcgg tggccgtgcg
     1141 aatcatcgtc gaccgtgagc gggaaatccg ggccttcgag ccggagagca gctttaaggt
     1201 tacggcgcag ttcagcgtcc acggcgagtc gtttccggcc gaactgacca accgcttgga
     1261 cagcgcggag ggcgccgagc agtggctaca gagcgtccag gacgcggtct ttaccgtcgg
     1321 caacgtcgcc accaaacccg gcagccgcag tcccggcgcg ccgttcacga ccagcacact
     1381 ccagcaggaa gcctcccgcc gcctcggcta ctccgtgcgc cagaccatga cgatcgccca
     1441 gcggctatac gaagccggcc atattaccta catgcgtacc gacagcacaa cgctatccgg
     1501 acaggctatc ggcgcagcca aggactacat caccgctacc tacggtgcca attatcatca
     1561 ggtccgtcag tacaagacca agagtcagaa tgcccaggaa gcccacgaag ccatccggcc
     1621 gaccgatttc aagcgcttgg aagccggcga ggacagccag caggccaagc tgtaccagct
     1681 gatctggcaa cgggccatcg catcccagat gagcagcgcc gagctggaac gcaccgaagt
     1741 cactatcgat atctcaggac accaagagca gttcctggcc aagggcgaga tattgcgctt
     1801 tgacggcttt atgaaggtct acggtggcgg caaggacgac acgctgctgc cggacctgaa
     1861 gacgggcgat gacctctcgg ccgagagcat taccgccgtc gagacgttca gccgtccgcc
     1921 ggcccgtttc agcgaggcca gtctggtccg caaactcgaa gagctcggca tcggccggcc
     1981 gagtacctat gccagcacca tcagcaccat tcagagccgc ggctacatag agaagcgcga
     2041 cctcgacggt gtcgagcggc acgtccgcga aataactctg tccggaggtg aagttacgcc
     2101 gtccagccgc agcgagataa cgggcgccga ccgcggcaag ctggttccta ctcctatcgc
     2161 cgaagtgacg accgatttcc tcgtcaaata cttcccggca atcgtcgatt atgacttcac
     2221 ggccaccgtc gaggaggagt tcgacgaagt ggcccgcggc gaagccgaat ggcaccagat
     2281 gatcgccgac ttttataagg atttccactc cttggtactg gaaagcgccg atgtttcccg
     2341 cgaggaagtc agccaggccc gtctggtcgg caacgacccg aagagcggca agcccatcct
     2401 ggcccgcttc ggccgttacg gcccgatgct gcagcggggc gagaccgaga gcgaggagaa
     2461 gcccgatttc gcaccgttac cgaaagatac gactatcgac accgtcactc tcgagcaggc
     2521 cttgacagca ttccaactgc cgcgagtcgt cggcacgacc gaagatggcc aagagatcaa
     2581 agccaatgtc ggccgctttg gcccctacat tcagatcggc aagctgtacg tgtccatcaa
     2641 ggatcacgac ccgcatacca tcgatgagcc gacgtcccgc gagttgtaca aggccaagct
     2701 ggcagccgaa gccgccaaga acattcagaa cttcggtgct atcaaggtat tgaacggccc
     2761 gtacggcccg tacgtgaccg acggcaagaa gaacgcccgc atcgccaaag acgtggaccc
     2821 cgccaagctc accgaagcgg aagccgaagc cctcctgaag gccgccccgg ccaaaaagcg
     2881 ctttacaagg cggcgcaccg ccaaaaaata gcgtaagcgg tctgcaggcc gcacgcttac
     2941 agattataag gccatctttt gcaatcctaa cagagtatac tctggtaaga attttacaac
     3001 atattgtgtg cgaatacgtc tagttcgtgc tacaatagta ttagaaactt ttggtttgac
     3061 ttttaaaaaa ttaagtttta taattttgtt atattcttct gatgtaacac cttataaaga
     3121 acagggaagt agtatctagt atggccgaaa cgaccaccac tgcgcatagt ctcaacaccg
     3181 aacagcttgt taatgacacg ctggctacta ttgaccgtga acgcgagcgc gaaatcgtcg
     3241 cccgccgctt cggcctgttt gaacgcaaag agactctgga gcagattggt gagctgctgg
     3301 gtatcacccg cgaacgtgtc cgacagctcg agaaagccgt ggtcacccgt ctcaagacgg
     3361 cagcagagca ggggcacctg cagcacatcc cggaatttca gcagaccgtc agccgtgaac
     3421 tcgaaaaggt cgggggcatc gcccgcatca gcgagctcag caacacctta ggcgacagcg
     3481 gccggttgga gcaggcccgt ttggctttcc tgagcggcct gtgccccgaa ctgatcgtca
     3541 tcgatgatga cgacaactat taccacagtg tcggtatccg caagtctttc gacatcgcga
     3601 gcgccaaggg gcaggtcgaa aagctcatcg ccgttattaa gaagcttggt gaaccgaagc
     3661 ccatcgaaga tgtcgccagc gccgccggta tgagtgacgt caagcaggcg gctgccctgg
     3721 ctggcatcag taagcagctg gcaactctca acggccgctg gggcctggta aagtggccga
     3781 tggtcaaccc caagaacatc cgcgacaaga tctacgttat ccttaaggaa aacggcaagc
     3841 acatgcactt caacgagatc gccgatgcca tcaagaagtc ggacttccgc cgcaaagacg
     3901 tcacgaccca ggctattcac aacgaactga tcaaagacaa gcgcttcgtg ctcatcggtc
     3961 gcggtattta cgcgctcaag gagtggggct acgagaaggg cactgtcgcc gacatcatca
     4021 ccgaggtcct gcagaaggca ggggagcccc tgcaccgcga cgaaattgtc aagcgtgtgc
     4081 tgaagagccg tttcgtgaaa gaaacgacca ttctgttgaa cctgcagggc aaaccgcagt
     4141 tcaagcgcgt cgccaaagct acttacacac tcgccgaaga agagtagaca tacgccccat
     4201 ggccgcaccc gaagtgctta atgtaacacg gccggatttt gacccgctgg agcaccttgc
     4261 tccgtttgcg cgccatcaga acatcgcttt tcatcctgcg aagcccggca aaatcctccg
     4321 gtggtaccct caccaggaca cgtgggattg tttcgacagc cccaaaggag caaacagcgc
     4381 gttgcacatg aaccagcaac acctgggttt cttagcctca gcaacacgta ttgcggtgcc
     4441 caaacattat gatttcattg cccaagagcc gccttattct aatgagaacc ctgcctatgc
     4501 tgtgttctcg gaacttgatc tgatagcggg ctgcagaccc gttgatgcaa aagacgacgt
     4561 tcggctggta tgggaagccc taaccaagta ccactcctgg gttgtaacta ccgaccaacc
     4621 agtcatactg cacgatgcct tcaagctcga ccagtatcat gtgacgaata aaggtgtaac
     4681 gctcctcgat attgaccctt attttgcaga gaccagagga cacactatgg ggctggtccg
     4741 ccagcagctg agaaacattg gaacaatagt aagttgaacc ttttacgaac gtttattttt
     4801 gttcgtacgt atatgataca aatagcgaac aagcgcagtg tcatggttag tacgggcaac
     4861 acggctgatt tgggctaagc caaaagcggt atcgaactta gcggggcaga aataataatt
     4921 tttgaaagac taagtgtgtg gagtaggatt ttgagcgcgg aatctggggc agcagtgcat
     4981 gaattcttac atggacgaga tgtcgcacaa tatatctttt acgactctat cataggtgcg
     5041 ttgggaggta tcagtgaccg tcccctaaca ggggagtttt gacttaatac ggcaagtaac
     5101 cccgtttaat tgatccttag ccctcgcacg gatatgatgt tctgtgtaag gcatagttgc
     5161 ttcaactaga gattcacggt gccctcaaag cccatagcgt ttgtgatatg tctcaccaca
     5221 caatttgact tttccacagc attgtcgtga aaatttgctt tttcacgact ttggttataa
     5281 atatattttt ctcccccgct ttgctccctg ccacctaaaa tttgacatat gacccttccc
     5341 gttaatttat atagtttttt atagtctcag cgatagaaca tgaattatga acatattgcg
     5401 aacaagagca gggtgttgca gggggaatgg cttggaggcg ccaacgcttc cccgccacaa
     5461 tcacgcagac cggcgccatg agtgctacga actaaaccta gcgatttcta cttgtagtag
     5521 gccctgcccg ccaggcgcac gtcaatgtac tgagaagggg tagtcccctg ctcttgcagt
     5581 cgcttcataa cggccagcat cgtaccgacc tgagcttggg cgtgctggta gtcatgcagg
     5641 ttgaacttga cgtaataagg cttgccggcg acccgcacat ccagctcgta agctttttcg
     5701 ggcagggtta cggactggat actcaccgct ttggcacgta gttcctgcgc cacatagctt
     5761 atgaacgtaa cggaactagc gggtagcgcg tcagcacggg gctgcaactt cagaccgctt
     5821 ttatctatca ctgtcggtaa ctgctgcctc gaggttgggc cgagcgctgt ggcgcgatca
     5881 agcacttggc ctgtcgtgtc gatgacatag gaagtatcgg cttcctgcag aataagtgcc
     5941 ggctgcttgg gcgaaacgta gatgattggc cggtgaccca taagcggcaa cgtgacggac
     6001 accgcagcga gctcggggaa ctgctgtttc agactgtggc tcaggcgcgt cgtatcgacc
     6061 gtgatcttgt tggcattaag ccaggagcta cgcagcgccc ggctcgctgc cgtctggtag
     6121 acactcgctg gctgcagcac gtaatcggcg gagttcgagg tcagcggcac gatctgcggc
     6181 gtcggcgaca agtgcagaat gtagccgaca catatcagaa tcagggcacc cataaacagc
     6241 accgataggc gttgccgcca gaagcgcacg aactgctcgt gacgccgtac gtcctggtcg
     6301 cggggctgcg accggccgat gttatagtcc tgctcaacgc ggttggcatg atacgagaag
     6361 gcctgcggac gggcgctgcg ggtaacccgg cgggggggcg gtggcgttgc tttggcacgg
     6421 aacagcttca tgacttctct atacctgtgg gccaccggct atttcgagga taagcgccgc
     6481 caattcttcc gcggcgttgg gacgggcgaa acgggcgata tggtctgcca gctcgcggcg
     6541 cttggtagca tcatcaagca actcactgat gacatgtgtc aaccgacgtt cttgctcgat
     6601 ctgctcggaa gtcatcagga cgatcgcctg ctcgcgggcc aggatccggg cgttcttcaa
     6661 ttgatggccg cccacgagct ggccggccgg cacgatgaca cagaccttgc cttgggcggc
     6721 gaactccgcc agattagtgg ctccggcacg tccgatgata atgtcggcgg ctccgctgta
     6781 gcggtagaac tccgtcacga aaccgtgcac cagtacgcgc tgacgctctt cggcggtcag
     6841 cgcggtgtcg tacgcttggc cgatggctgc ttcgtggtcc cggccggcga catggatgat
     6901 atgcaggtcc gggtagcgct tgagcaggaa cggcgcattg gccacgaccg cctcgttcag
     6961 ctcttgggca ccgttgccac caccggtcac tagcaacagg cggccgtctt tggccacgcc
     7021 gacctgatgc ttataagctg cctgggtggc cgccgtaacc ggctggtagt cggccagcag
     7081 cggtatgccg accatcttgg tcttattgcg cgggtacgga tagctgtctt ccggcagggc
     7141 gacggcgtgc agccttgccc agcgggcgat caaccggtta gccaagctgg gtacgctgtc
     7201 cgaatcatgc gtgatatacg ggacgcccgc caaatgccca cctagcgcca ccggtacact
     7261 tacgaagcca ccccggctga agacgaccga cggtttaagg cggcgcatca agcggtagct
     7321 ttgcactagt ccgataagca cgtagactgc atcgcggatg tttttgaact gcgtcggcag
     7381 gtccagcagc tggcgccacc cttctccgtg gtagcggcgg aacttccctg cccgcaccgt
     7441 gtacacttcg tcaacattcg gatcggccgc cggaatatcg gccagcttat caccggcctg
     7501 gccgatatac acgatgcgta aacccggatc aagctgtttg agactgctag cgaccgccaa
     7561 aattggggtg atatgccccc ctgacccgcc gcctgtcacg actatagtca tttccgctcc
     7621 tgtcgttagt taacgtcacg tacgaagtat agcgcgagat ctggaagacc aggccgacgg
     7681 ccgctgccgt gaacaccacg ctggtaccgc cgtagcttat gaaaggcaac gtgatgcctt
     7741 tgagcggcag caagccgatc atggccccta tgttgatgag cgcctggata ctgagccagg
     7801 tcagcactcc gacgaccagc agccggctga agtcatccgg cgagcgctcg gcgatcgact
     7861 tgagccggtg gaagaacgcg atgaatatcg ccagcaggat caggacgccg ataaaaccga
     7921 atttctcggc ataaatggcg aagatcgagt cgttggccgc ttccggcaga tagccgtagg
     7981 cttggacgct ccgacctaaa ccgagcccga tcatcccccc tgaaccgacg gcgatgagcg
     8041 cctgacactc ttggtacccc gacgtctgac aatcggcttc cgggtgcaga tacgtcatga
     8101 gccgttcccg tcggtacgga gtggaggaaa tagccagtat caatccgatg gcgacgatgc
     8161 cggcgatggc caacacccgc ttcatgggca ggccggctac gaaagccatt acgcccatca
     8221 ttagaacgat taccccggta gaaccgaggt cgctttgcac accggctatc atcaccccga
     8281 tgatgccgag cgcgataagc agcggtttga gcgtggcgtt gaagtcggcc atcttgccgc
     8341 tgcgcatgcg gaaacttaag aaacccgaca gccagatgag cagcgcgaat ttcagcagct
     8401 cgaccgactg gaacgaaaag ctccccaggc gcacccagcg atgggcagga tagttcgggt
     8461 tgaccggcat cacgatagct accagggtcg cgagggcggc tatgaccagc aacggtttgt
     8521 aggcctggcg ccaccagctc agcggcacct gggacgtcac gacgaacgcc acggcgctga
     8581 gcccgatggc aatcagctgc ttgccgacgt aatagttact gctcacatgg gccgaggcac
     8641 tcaaggcagg gctgatagcg tagaccacga tgagaccgat gcagagcaag atgacacaga
     8701 gcgcgaccag ccaataatcc ggcttatggc gccgtccgag ctcgctgacc ggctcggctc
     8761 tgcggaaggc ttgaccgccg ggccgccgtt cgccctgccc cggctgccag ctcatcggta
     8821 cctgcctaag acgaataagg ccagaccgag tacggcgcca atctgaccga gaatccaaaa
     8881 acgcatcgtc accttggtct cgggccaccc catggcttcg aaatggtggt gtatcggtga
     8941 cgataggaac actttcttgc catggcgcag cttccgcgac agccggttga tgataaccga
     9001 gccggtctcc atgacgaaca cgaaaccgat gatcggcagc acgtagacgg tattggtctg
     9061 catggcgatg ataccgagcg ccgtgccgag cgcgaacgaa ccgacatctc ccatgaagaa
     9121 gcgggccgga taaatgttga accacgtata gctgagcaac gcgccgacca ccgtcaggca
     9181 aaagcccgcc aaggcgtact tattctcgat gccggcaata agggcgtagg cgccgaaggc
     9241 cgaagccagc aggccacctg ccaaaccgtc gagaccgtcg gagatattga cggaattagc
     9301 cgtcgccact acgacgagcc agaagactgc gatgacggcg atgccgatat gccagttatg
     9361 gatgcccggc aggtacagcg tattgacacc cagtttggcg tagaaccacc aaccgccgac
     9421 cagcgcgaca aggctgtaca gggcgaactt gatacccgac ttcatcccgg caatggaagt
     9481 attgacgccc ctgatgttca tgacgtcatc taccaggccg atcagaccgg ccgctagcat
     9541 accggccagc ggcagccagg tttc
//
LOCUS       DASSFD010000081         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1643214, whole genome shotgun sequence.
ACCESSION   DASSFD010000081 DASSFD010000000
VERSION     DASSFD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643214"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1007)
                     /locus_tag="VFH39_01860"
     CDS             complement(99..1007)
                     /locus_tag="VFH39_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HET6924555.1"
                     /translation="MNKQLETKLKDLPKEPGVYFHKDASGEIIYVGKAAVLRNRVRQY
                     FQNSRARDPKTEALVSEIVDTDWMVVESELEALFLEAEMIRRYMPRYNILLRDDKSMS
                     YIRIDYDSDYPTVTTTRRPLDDGARYFGPYFSTYGVRQALKLLRKVFPFASKRPVGEK
                     RASLYYHLGLDPGLEEGRTSLEDYRANLRKLISVIQGKRTAILKEIERDMKRAAKAHE
                     FEQAARLRNQLVALQALGKQVVFSDKEFMDISKDHALSELVTLLNLLSFPRRIEGYDI
                     SHMQGTNVVASMVVFTNGVSDKGEYR"
     gene            complement(1438..2334)
                     /locus_tag="VFH39_01865"
     CDS             complement(1438..2334)
                     /locus_tag="VFH39_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET6924556.1"
                     /translation="MQKILLYYKFTPLTDPEAVRLWQKALCESLNLKGRILISRHGIN
                     GTVGGELADLKRYVRAFKAYPGFKQTQFKWSDGDRDHFPKLKVKVRPEIVAFGAGEEL
                     QVDETGVVDGGTHLKPTEVHELVNRYGDEVIFFDGRNAYEAAVGRFKNAIVPNTATSK
                     DFIAELDSGRYDHLKDKKVVTYCTGGIRCEILTPLMKHRGFKDVYQMDGGIVKYGEQY
                     GDDGLWEGSLYVFDERMNLKFSDKAEDIGECIHCASKTSNYENCALPSCNELVLICEN
                     CLVHPDRRYHRTTCRQRGTVAN"
     gene            complement(2355..>3395)
                     /locus_tag="VFH39_01870"
     CDS             complement(2355..>3395)
                     /locus_tag="VFH39_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924557.1"
                     /translation="EEAAHYERRAETRGLLIGGLVGYFIGRRRGRIKTEKRLLPVQEK
                     LEKQVKTLHETIADKEFQIRQLAARRHVQAKRQATQERPKPAERPPVPQPEVVPVAKT
                     ADTPSTVVEAKSRPMPETPRYVEQVPAGPMVQVAAEVAATTAAMTAAATEQIHRKQAA
                     ERLPATPPPNEAVARDAAADRVKHMSRRQLLAASEQVVISGERLSEVYESKRISEAGL
                     RNVMMEHARGGDAEKLLKQEILNKEMSYERDPQLRKRLTSGVSAASHGLAGAGAAAGA
                     ATANLAHKAVPKVAATAERTKDIGKKVVQQAVNTGKKLDDPGKPLQQVIVRTWATVTI
                     LLAIIAIILLFR"
BASE COUNT          579 a         1033 c          989 g          794 t
ORIGIN      
        1 ggcttgcccc acgctttcac gttcttttcg ctcaggcggc gcgtactggt ctcgtgcatg
       61 ttatagaagt cgttattatg ctcgatgcgc gttttgaact agcggtattc gcccttgtcg
      121 gacacgccgt tggtaaacac caccatgctg gcgacgacgt tcgtcccctg catgtggctg
      181 atatcgtagc cctcgatccg ccgcggaaag cttagcaagt taagtaatgt cacgagctca
      241 ctcagcgcat gatccttgct gatgtccatg aattccttgt cgctgaagac gacctgtttg
      301 ccgagcgcct gtaacgccac cagctggttg cgcaaccggg ccgcctgctc gaactcgtga
      361 gctttggccg cccgcttcat atcgcgctcg atctctttca gtatcgccgt ccgtttgccc
      421 tggatgaccg atatcagctt gcgcagattg gcacggtaat cctccaggct ggttctgcct
      481 tcttccagac cggggtcgag ccccaggtgg tagtacagac tcgcccgttt ttcaccgacc
      541 ggacgtttgc tggcgaacgg aaacaccttg cgcagcagtt tgagcgcctg ccggacaccg
      601 taggtactga agtaagggcc gaagtaacga gcgccgtcgt ccagcggccg gcgggtcgtc
      661 gtgaccgtcg ggtaatcact gtcgtaatcg atgcggatgt agctcatcga cttatcgtcg
      721 cgcagcagga tgttgtagcg cggcatgtag cggcggatca tctccgcttc caaaaacagt
      781 gcttccagtt cactctccac caccatccag tcggtgtcga cgatctcgct caccaacgcc
      841 tcggtcttgg ggtcgcgcgc ccgcgagttc tggaaatact gccgcacgcg gttgcgcagc
      901 accgccgcct tgcctacata gatgatttcg cccgaggcat ccttgtgaaa gtacaccccc
      961 ggctctttcg gcaggtcttt cagtttcgtc tccagttgct tattcaccgc tattattata
     1021 cggcaatcac gaccccggca taagcattca cccgggcact accggtgcgg cttgacaaca
     1081 agtaaccata agcgtattac gggactatga ccgagcgtcc cgctccggca gaaacaaaca
     1141 cttccgcccc ttcgtccgag ctggttctgc gcctcactgc cgatatcact atttctttgc
     1201 caagcgaatg cgtcggccgc ctgcggcccg acactcccca ggaggctgcc cgccgggccg
     1261 ccgagctgga gtgggcgcag gcagcattcc gccgacagat gagtcagccc tgtagccatc
     1321 cgcagaaacc ggctgttgcg gccgctgaga ctgccgtgtc agccgagtcc gagcttcaaa
     1381 atgtcgtgcc gctcaagccg caacaggtcc gacatatcgg acaggcagcg tttctggcta
     1441 attggccaca gtgccgcgct gtcggcatgt ggttcggtga tagcgacggt ccggatgtac
     1501 tagacagttt tcacagataa gcaccagctc gttacagctg ggcagtgcgc agttttcgta
     1561 gttgctggtt ttggatgcgc agtggatgca ttcgccgata tcttcggctt tgtcgctgaa
     1621 cttcaggttc atacgctcat caaagacgta gaggctgcct tcccagaggc catcgtcgcc
     1681 atactgctcg ccgtacttca ctatgccgcc gtccatttgg tagacatctt tgaacccccg
     1741 gtgcttcata agcggtgtca ggatctcgca gcggatgccg ccggtacagt atgtgacgac
     1801 ctttttgtcc ttcagatggt cgtagcggcc actgtcgagc tctgcaatga agtctttgct
     1861 ggtagcagta ttgggtacta tggcgttttt aaaacggcct acagcggcct cgtaggcgtt
     1921 ccgaccgtca aaaaagatga cttcgtcgcc gtatctgttg acgagctcat gcacctctgt
     1981 cggtttgagg tgcgtaccgc cgtccaccac ccctgtttcg tcaacttgca attcctcgcc
     2041 agcgccgaaa gcaacgattt ccggccggac tttcaccttc agcttcggga aatggtcgcg
     2101 atcgccgtcg ctccacttga actgcgtttg tttgaagccc ggatacgcct tgaaggcccg
     2161 tacgtaacgc ttaaggtccg ccagctcccc acccaccgtg ccattgatac cgtggcggct
     2221 gatgagtatg cggcccttca agttgagcga ttcgcacaac gccttttgcc acagccgcac
     2281 tgcttccggg tcggttagcg gagtgaattt gtagtacagg agtatcttct gcatcgggta
     2341 tcaattatac cgggttagcg gaagagtaag ataatggcga tgatggccag caggatggtg
     2401 acggtcgccc acgtgcggac gatgacctgc tgcagcggtt tgccgggatc gtccagcttc
     2461 ttgccggtat tgaccgcttg ctggacaact tttttgccga tgtccttggt gcgttcggcc
     2521 gtggcggcga ctttggggac ggccttgtgc gccaggttgg cggtagcggc gccggctgcc
     2581 gcaccggcac ccgccagccc atgactggcg gcgctgacgc cgctggtaag gcgcttccta
     2641 agttggggat cacgctcgta cgacatctct ttgttgagga tttcctgctt caggagcttt
     2701 tcggcgtcgc cgccccgggc atgctccatc atgacgttgc gcaggcccgc ttcgctgatc
     2761 cgcttcgatt cgtacacctc gctcagccgt tcgccgctga tgacgacctg ctcgctggcg
     2821 gccagcaact gccgacgact catgtgcttg acgcggtcgg cggcggcatc gcgcgcgacg
     2881 gcttcattcg gcggcggtgt ggccggcaaa cgttccgctg cttgcttgcg atggatctgc
     2941 tcggtagcag cggccgtcat cgcggcggtg gtagcggcca cctcggcagc gacctggacc
     3001 atcgggccgg ccggcacctg ctcgacataa cggggagttt cgggcatcgg ccgggactta
     3061 gcttctacga ccgtagaggg tgtatcggcg gttttcgcta cagggactac ttccggctgg
     3121 ggcaccggtg gtcgctcggc cggcttcggt cgttcctgtg ttgcctgccg cttagcctgg
     3181 acatggcgtc gggccgccag ctggcggatt tggaactctt tgtcggcgat ggtttcgtgc
     3241 agggtcttca cctgcttctc gagtttttcc tgcaccggca gcaggcgctt ctcggtcttg
     3301 atacggccgc ggcggcggcc gataaagtag ccgacgaggc caccgatcag caagccgcgg
     3361 gtctcggccc ggcgttcata atgagcggct tcttc
//
LOCUS       DASSFD010000082         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1664239, whole genome shotgun sequence.
ACCESSION   DASSFD010000082 DASSFD010000000
VERSION     DASSFD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664239"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFH39_01875"
     CDS             complement(<1..643)
                     /locus_tag="VFH39_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924558.1"
                     /translation="MRASPVMKRAIRLGRQYWLLSVTILVSILGLILQLVGWKYAAHW
                     LLGLMDLLAAIVWLGHAWKELQSGSYRVNYVAIIAVVTAVVVHAYWVGIALVAAQSLL
                     VILLARLTRRAHHELDLLDEVEPQMAQVLQGRKLSEVKASSVVAGSKFTVGPQDTVPA
                     DGVILEGSATVDETFLTGEPRPTAVGVDAHVLAGSRVTEGNLTVRATRDYKNSQ"
     gene            complement(680..1318)
                     /locus_tag="VFH39_01880"
     CDS             complement(680..1318)
                     /locus_tag="VFH39_01880"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HET6924559.1"
                     /translation="MTTVTRRLDTPTLPWTIAVIVSALAFYVWGSSLEWRFAGLSPYQ
                     LFPLFGLLAFSLMWGHYVVGMLCRLFLDRGKLDDYFRLTGYAVLVLILLHPGILVYQR
                     FRDGFGLPPHSYETYVAPGLAWVTLLGTVSLLIFLAFELHRWFKDASWWKYVAAAGDL
                     AMLAVFYHGLELGTHTHVQWFRVVWYFYGVTLVGCLLYKYWFILRKRDKVGA"
     gene            1373..1762
                     /locus_tag="VFH39_01885"
     CDS             1373..1762
                     /locus_tag="VFH39_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924560.1"
                     /translation="MTKPLQLTLVLALLVLAVVGISLLGRPEAGSDACGAANGPLHTI
                     VITNGHPSSGTVQGNLCDRLTFVNDDHLTREIAFGPHEDHVSYDGIAERFLNYGQSFT
                     ITLNETGTYHWHDHLHDDVEGYFTVSR"
     gene            complement(1819..1895)
                     /locus_tag="VFH39_01890"
     tRNA            complement(1819..1895)
                     /locus_tag="VFH39_01890"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1859..1861),aa:His,seq:gtg)
     gene            2156..2488
                     /locus_tag="VFH39_01895"
     CDS             2156..2488
                     /locus_tag="VFH39_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924561.1"
                     /translation="MELSPTGGENADSESLIRQDGSLNEQLLAEQYGLGPEEAMQEVA
                     FGAYSGTLAQALADDRCPVGGMVRAAYTEHGLEGVTEKLHTLGQLDPRFNVEVSPATL
                     QREQLKKN"
     gene            2608..>3134
                     /locus_tag="VFH39_01900"
     CDS             2608..>3134
                     /locus_tag="VFH39_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924562.1"
                     /translation="MKPELPAAVLAAREARAETAPLYDEPVAAHQVAVLMERQRNEAQ
                     LHFTEETPSDSDHNAKADIKPETDNDQSTGRPLKVSVTPQRHSKKSTEAGSELLAKPA
                     HAEHTAVDPQPEAESRIATPLEREPSALPEIPETEERRNHQSETVLLMPETVIDLWPD
                     ILISEEAGLEGEELTS"
BASE COUNT          766 a          914 c          852 g          602 t
ORIGIN      
        1 actgactgtt cttgtaatcg cgtgtcgctc ggacggtcaa attgccttcg gtaacccgcg
       61 aaccggccag gacgtgtgca tcgaccccta cggccgtcgg gcgcggttca cccgtcaaga
      121 atgtttcatc tacggtagca gagccctcca ggatgacgcc atcggccggt acggtgtcct
      181 ggggaccgac ggtgaacttg ctgcccgcca ccacgctgct ggcttttact tcgctcaact
      241 tgcggccttg caacacctgg gccatctgcg gctcgacctc atcgagcagg tccagctcgt
      301 ggtgggcccg gcgcgtcagt cgggccagca ggattaccag caacgactgc gctgccacca
      361 atgcgatacc gacccaatag gcgtgcacca ctacggccgt caccactgcg atgatggcca
      421 catagttgac gcgatagcta ccgctctgca gctctttcca ggcgtgcccc agccacacga
      481 tggcggccag caggtccata aggcccagca gccaatgggc ggcgtatttc cagccaacca
      541 gttgcaatat cagtccgagt atagaaacaa gaatcgttac gctcagcaac caatactgtc
      601 gtcctaggcg gatggcacgt ttcatcacgg gtgaggccct catttaaact tgtattcagt
      661 atagcaagca gagcggggtt caggcaccaa ctttgtcacg ttttcgcaga atgaaccagt
      721 acttgtataa caggcagccc accagtgtga cgccataaaa ataccagacg accctgaacc
      781 actgcacgtg ggtgtgggta ccgagctcca gcccgtgata aaacaccgcc agcatcgcca
      841 ggtcgccggc ggctgccacg tatttccacc acgatgcatc tttgaaccag cggtgcagtt
      901 cgaaggcgag gaatatcagc aagcttaccg tgccgagcaa cgtcacccag gccaggccgg
      961 gcgccacata cgtttcgtag ctgtgtggag gcaggccaaa accgtcgcgg aagcgctggt
     1021 agacgaggat tcccgggtgc agcagaatca gcaccaaaac cgcataaccc gtcagccgaa
     1081 agtaatcatc cagcttaccg cggtccagaa ataaacggca gagcatgccg acgacgtagt
     1141 gcccccacat gaggctgaag gccaacaacc cgaacagcgg gaaaagttga taagggctta
     1201 agccggcgaa tcgccactcg aggctacttc cccagacgta gaatgccagc gctgatacga
     1261 tgaccgcgat ggtccagggc agtgtaggcg tatcgagacg acgcgtgaca gtggtcatag
     1321 ctcaatcata cgctccctga gcgcgcatgg gatgtgatac agtcagaaca gtatgacgaa
     1381 gcccctgcaa ctgacccttg ttttagcgct cttggtactt gccgtcgtgg ggataagcct
     1441 gctcggtcgc ccagaagccg gcagtgatgc gtgcggtgct gccaacggtc cgcttcatac
     1501 tatcgttatc acgaacgggc acccctcgtc cggtactgtc cagggcaacc tatgtgaccg
     1561 gctgactttc gtcaatgacg atcacctgac acgcgaaatc gccttcgggc cgcacgaaga
     1621 ccatgtgtct tacgacggca tcgctgagcg gttcctgaat tacggccaat cgtttaccat
     1681 cacgctcaac gaaaccggca cctaccactg gcacgaccac ctgcacgacg atgtcgaagg
     1741 ctacttcacg gtcagccggt agacgattcc gccccgggcc aaataaatag accaagcttg
     1801 tgcttggcct acttaatctg gggcgagtga tgggaattga acccacgacc ttctggacca
     1861 caatcagacg ctctaaccaa ctgagctaca cccgcctcgt cctcccgtac gcctatgcct
     1921 tgttttgccg cgggcggcaa aagcttcggc ctatggctac ggtcgtcctc tcagaatcgc
     1981 attcagtcgc tatcgctcct tggattgcgt ttctggattc cgctgcacaa tagtatgcag
     2041 aaaacagagg tgattatatc tgttatcgct aaaaatttca ataacacatt acggtatgcg
     2101 ttacctggct gattattatc gacttttaag ctttaagcgc ttatgataat gacatatgga
     2161 attgtcaccc accggtggtg aaaacgccga cagtgaatca ctgatacgtc aggatggttc
     2221 actgaacgaa cagctgttgg ccgagcagta cggtcttggc ccggaggagg cgatgcagga
     2281 agtggccttt ggggcctaca gcggcacact ggcccaggcc ttggctgacg atcgttgccc
     2341 ggtcgggggt atggtcagag cggcctacac cgagcatgga ctcgaaggcg tgacggagaa
     2401 attgcacacg ctaggtcagc tcgacccgcg cttcaatgta gaagtttcgc cggccacttt
     2461 gcagcgcgaa cagctaaaaa aaaattagat ccgcctgccg aaacagctca gattcagcat
     2521 gagcgccgag cggccaccga taccgtagct gccgaaacta tcaccccgct gcaaaaagca
     2581 ctgctagatc aaaacagcgt ggtgaatgtg aagcctgaac tgccggcagc ggtcttggct
     2641 gcccgtgagg cacgtgcgga aacagcgccg ctatatgatg agccggtggc ggcccaccag
     2701 gtagcggtac ttatggaacg tcagcgcaac gaagcacagt tgcacttcac ggaagagaca
     2761 ccgagtgact ctgatcataa cgccaaagca gatataaagc cggaaaccga caatgaccaa
     2821 agtacgggca ggccgcttaa ggtatcagtt acaccgcaaa ggcattctaa aaagtctacg
     2881 gaagcaggct ccgaacttct tgccaagcca gctcacgcgg aacacaccgc tgtggacccg
     2941 cagccggagg ccgaaagccg tatcgccacg ccgcttgaac gcgaaccatc ggccctcccg
     3001 gaaataccgg aaacggaaga acgaaggaat caccagtcag aaaccgttct cttgatgccg
     3061 gagacggtga tagatctgtg gccggatatt ttaataagcg aggaagccgg cttagagggc
     3121 gaagagttaa cttc
//
LOCUS       DASSFD010000083         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_257191, whole genome shotgun sequence.
ACCESSION   DASSFD010000083 DASSFD010000000
VERSION     DASSFD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257191"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFH39_01905"
     CDS             <1..1084
                     /locus_tag="VFH39_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HET6924563.1"
                     /translation="TFTLSDRSQKTYPALLGRRLLHGKFLVDVTDGDPLHEAESDKAA
                     ALERDLRTIQRKKGGGLKIAILSKGPGNYSTRRLKEEIQARGHEVRVINYAKCYVTLE
                     SNKPVIRYEGEELRDIDVIIPRIASNLTAYGSSIVRQFEMQNVFTTTTSIAIVRSRDK
                     LRSTQLLAKAGVGIPKTVFGRETADLDDVLEQVGGAPVIIKVARGTHGNGVVLAETRK
                     AAKAVMQAFYVEGVSFIVQEYIAESAGADIRAFVVNGKVVASMKRQSLDDDFRSNLHQ
                     GGEGTTIRLTEEERKTAQRAAKAMGLPICGVDMMRSERGPLVLEVNSSPGFGIEKVTG
                     RNVAAKIIEYVEQHAKAGRRKDKVGA"
     gene            1127..1627
                     /locus_tag="VFH39_01910"
     CDS             1127..1627
                     /locus_tag="VFH39_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924564.1"
                     /translation="MSPEVIIVGLMAVPVVLLFALQVNAALVFLSLCLGDVLVQFAGH
                     DASSIISGAAVNAHATTSSIQLALLLAPAVLTVLFMIKTVHGKYKKIFNILPAAGVGL
                     LTALLAVPLLPPGLSHNVTAASLWQNILQFQSGIMALSTLVCLLFLWMQRPKKPGEDK
                     HGKHPH"
     gene            1914..1990
                     /locus_tag="VFH39_01915"
     tRNA            1914..1990
                     /locus_tag="VFH39_01915"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1948..1950,aa:Lys,seq:ttt)
     gene            2117..2596
                     /locus_tag="VFH39_01920"
     CDS             2117..2596
                     /locus_tag="VFH39_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924565.1"
                     /translation="MAERRLLELIYTFFVGVLLALFVGVGINTFYAQPVAPRYPIELE
                     VPRKELSAQQLLLQRQFDKRNEAYLATLKTYNRNVSMLALAAAVILLGLSLLAEKRLK
                     LVANGVMLGGLLTLFYSIGRGFASESSNYTFWLVAISLVIVLLLGFRKLAGRQGSTP"
BASE COUNT          597 a          715 c          752 g          557 t
ORIGIN      
        1 gacgtttacg ctttcggacc gttcgcagaa gacctacccg gccttgctgg gccggcgttt
       61 gctgcatggc aaattcctgg tcgatgtgac cgacggtgac ccgttacatg aagcggaaag
      121 cgataaagcg gcggcactgg aacgtgacct acgtaccata caacgaaaga aggggggtgg
      181 tttgaaaatt gcaatcttat cgaaggggcc gggaaattat tcgacccgtc ggctgaagga
      241 agaaatacag gcacgtggtc atgaggtgcg cgtcatcaac tacgccaagt gctacgtgac
      301 gctcgagagc aataagccgg ttatccggta cgagggcgag gagctgcgcg atatcgacgt
      361 tatcattccg cgtatcgcct cgaacctgac ggcctatggc tcgtccatcg tccggcagtt
      421 cgagatgcag aacgtcttta cgacgaccac ctcgattgct atcgtccgtt cgcgcgataa
      481 gctgcgcagt acgcagctgc tggccaaggc cggtgtaggt atccccaaga cggtcttcgg
      541 ccgcgaaacc gccgacctcg acgacgtatt ggagcaggtg gggggcgcac cggtgatcat
      601 caaggtcgcc cgcggtaccc acggcaacgg cgtcgtgttg gccgagaccc gcaaggccgc
      661 caaggccgtg atgcaggcct tctatgtgga aggcgtgagc ttcatcgtgc aggaatatat
      721 cgccgagagc gccggagctg acatccgggc gttcgtggtc aacggcaagg tggtggccag
      781 catgaagcgc cagagtctgg atgacgattt ccgctccaac ctacaccagg gcggagaagg
      841 tacgaccatc cggcttaccg aagaggagcg caagacagcc cagcgggcgg caaaggctat
      901 gggtctgcca atctgcggcg tcgacatgat gcgctctgag cgcggtccgc tggtgctgga
      961 agttaatagc agcccaggct tcggtattga gaaagtcacc ggccgcaatg tggcagccaa
     1021 aatcatcgag tatgtcgagc agcacgccaa agccggccgg cgcaaagaca aagtcggcgc
     1081 ctgaaaatcc gtctgtttca cataacgctc atgctatgat gagcatatgt ctccggaagt
     1141 catcatcgtc ggcctgatgg cagtgcccgt cgtgctgctg ttcgcgctgc aggtaaacgc
     1201 cgcgctggtg ttcctgagcc tgtgcctggg tgatgtgctg gtgcagttcg ccggccatga
     1261 cgccagcagt atcatttccg gggcggcagt caatgcgcac gctaccacca gctccattca
     1321 gctggcactg ctgttggcgc cggccgtatt gacggtgctg ttcatgatca agaccgtgca
     1381 cggcaaatac aaaaagatat tcaacatcct gccggcagcc ggcgtcgggc tactgacggc
     1441 cttgctggct gtccccctgt tgccacccgg cttgagccat aacgtcacgg cagccagcct
     1501 atggcagaac attctgcagt tccaatcagg catcatggcg ctcagcacct tggtctgcct
     1561 gttattctta tggatgcagc ggcccaagaa gccaggcgaa gataaacatg gcaagcatcc
     1621 tcactgacat ctgttaacag attgactttt ccgccaaaaa acccataatg taagaagtta
     1681 ttttccctcc aaacaaggaa aatgcagccc accaaaaccg caatccaaga agcgatagcg
     1741 actgaatgcg gttttgagag gagcaacgaa gccgcaagtc gaagcttttg ccgttcacgg
     1801 caaaacgaga tagcggcgta ggttgcgacg agggccccat atgcttcgca tatgaccccc
     1861 aaatcctcgg aaagaaattc tatgtaaaca caaaattatc tttcctgcgc tttgggccca
     1921 tagctcagcc ggttagagca gcggcctttt aagccgcgtg tcgtgggttc gaatcccact
     1981 gggccctcca cgctaagacc cgacccatgc ggtcgggttt ttgcgtggct ggacctaccc
     2041 cccacttagg ggcagtgacc gcaatcacag ccgcagcgct atcgccgctt tagactgcgt
     2101 gtaaatggag gcgaatatgg ctgaacgcag actccttgag cttatctata cattcttcgt
     2161 tggagtgttg cttgcactgt tcgtcggcgt cgggataaat actttttatg ctcagccggt
     2221 cgcaccgcgt taccctattg aacttgaagt gccgcgtaaa gaacttagtg ctcagcagct
     2281 gctactgcaa aggcagttcg acaaacggaa cgaggcatac ctcgccacgc ttaaaactta
     2341 taaccgaaat gtttctatgc tggctttagc cgcggcggtt atattgctgg gactaagctt
     2401 attggcagag aagcggctga agctagtggc gaatggagtg atgctcggcg gcctgctgac
     2461 gctgttctat agcatcggca gaggcttcgc ttcggagagt agcaattaca ccttctggct
     2521 tgtggctatc agcttggtga tcgtactctt gctgggattc cgcaagctgg ccggaaggca
     2581 aggctccact ccatagtgtg atatgtaaag attttgacaa c
//
LOCUS       DASSFD010000084         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_570927, whole genome shotgun sequence.
ACCESSION   DASSFD010000084 DASSFD010000000
VERSION     DASSFD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570927"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFH39_01925"
     CDS             complement(<1..266)
                     /locus_tag="VFH39_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET6924566.1"
                     /translation="MKTITFVTGNPGKLAEWQRLFPADIRLESADVDLDEIQSLDLEA
                     IAIDKAKRAYAQLQRPVLVEDVAAGLDSLGGLPGPFVKYFEKSIG"
     gene            complement(263..1252)
                     /locus_tag="VFH39_01930"
     CDS             complement(263..1252)
                     /locus_tag="VFH39_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6924567.1"
                     /translation="MAKKSENFLQKVVRLIDEVQQDNRWLSFPYAVIKKYGDDEAGYQ
                     GALMTYYGFLSLFPLLIVATSVIDLITKHNAALREKLFHAISNYFPAFGSDLQASVHG
                     AHKTGLALIIGLLITLYGAKGVADAVQHALNHVWEVPKQERPAFPKGPLKSLALIIGG
                     GIGFAATAVLSSYATAALGHSFYFRLLPIVLSFFMLFAVFTFVYRVGLSAKRSWNELL
                     PGAAVAALGMQILLTFGTYLVTHQLKHLSGAYGRFGLVLAILFWIYLQAQVFLYAAEI
                     NSVHALRLHPRSITQDPLTEADRRAFKLYAKRETVKAPPEEVHVEFKDPEASR"
     gene            complement(1320..2156)
                     /locus_tag="VFH39_01935"
     CDS             complement(1320..2156)
                     /locus_tag="VFH39_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924568.1"
                     /translation="MPTNPEVYPEHIVAPERLELARTILGKELRKLFLGLADDSWRAM
                     DDSVRGWAEQPVKYHLEDGGPRDFWDSFAAFMESIKLKPDFTRWLTAENVRWSKQHLD
                     PRELILTSPLDQLRRIPDIDLRTGMHFSEIADLLNRNPEARATQKKLIDEHSTDPAQD
                     TYPIIVQIDPELGLKIMDGNRRTLKAVIYGKDGIDAWVAEHDGDDLRDFWVPVNDLYQ
                     LAVQFKTAQKDGDTAGQEAVARILRQVFAHSQVAKQTYEARVRDEAGYTSELYDMALR
                     QR"
     gene            complement(2179..2255)
                     /locus_tag="VFH39_01940"
     tRNA            complement(2179..2255)
                     /locus_tag="VFH39_01940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2219..2221),aa:Arg,seq:tct)
     gene            complement(2380..2454)
                     /locus_tag="VFH39_01945"
     tRNA            complement(2380..2454)
                     /locus_tag="VFH39_01945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2419..2421),aa:Gly,seq:tcc)
     gene            complement(2563..>3264)
                     /locus_tag="VFH39_01950"
     CDS             complement(2563..>3264)
                     /locus_tag="VFH39_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924569.1"
                     /translation="MDPWLTSGACNLAKAGTFQKVFSHHSHFFAGGDIEIGKLAQVMG
                     FKLKHIRFTFYTAAPDTVRGWFNQRIIWFAGGVRHHVINIASYGWYHFFMLFYNSLLI
                     YLLLPLRWVELINFPFTLLALIVLSWFYTLLLITGKHKWRPVYLLLPFYSFVQSMIIL
                     PLGFMRYFKIAWQQRSFGFLRYDLMNVGLFTRTLNRSLNLSSAAFIIYAAIAFTLTRW
                     HYWTQHGVILKDLVR"
BASE COUNT          692 a          876 c          964 g          732 t
ORIGIN      
        1 cctatcgatt tttcgaaata ctttacgaag gggcccggta atccgcccag gctgtccaac
       61 ccggcggcga catcttctac aaggaccggt cgctgcagtt gggcgtaggc ccgtttggcc
      121 ttgtcgatgg cgatggcttc gaggtccaga ctttggatct catccagatc gacatcggcc
      181 gattcgagtc ggatgtcggc ggggaacaac cgctgccact cggccagctt accggggttg
      241 cctgttacga aggtgatggt tttcatcggg acgcttccgg atctttgaac tccacgtgga
      301 cttcctcggg cggcgctttg acggtctcac gcttggcgta cagtttgaac gcccggcggt
      361 cggcttcggt cagcggatcc tgggtgatgc tgcgcgggtg cagccgcagg gcgtggacgc
      421 tgttgatttc ggcggcatac aggaacacct gggcctgtag ataaatccag aacagtatgg
      481 ccagcactag cccgaagcgc ccgtaggcgc cgcttaagtg tttaagctgg tgggtgacca
      541 ggtaggtgcc gaaggtgagg agtatttgca tgcccagtgc ggccaccgcg gctccgggca
      601 gcaactcatt ccaactgcgt ttcgctgaca ggcctacccg gtagacgaac gtaaataccg
      661 cgaacagcat gaagaagctg aggacgatcg gcagcagccg gaagtagaaa gagtgtccca
      721 atgccgccgt ggcatagctg gacagcaccg ccgtggccgc gaacccgatg ccgccgccga
      781 taatgagcgc cagacttttg agcggtccct tcggaaaggc cggccgctcc tgcttcggca
      841 cttcccagac gtggttaaga gcgtgctgga cggcgtcggc cacacctttg gcgccgtaca
      901 gggttatcaa taggcctatg atcagtgcca acccggtctt gtgcgcgcca tggacgctgg
      961 cttgcagatc gctgccgaac gccgggaagt agttgctgat ggcgtggaac agcttttcgc
     1021 gcagggcagc gttgtgcttg gtgatgaggt cgatgaccga tgtggcgact atgagcagcg
     1081 ggaacagcga caaaaagccg tagtacgtca tgagcgcgcc ctggtagccg gcttcgtcat
     1141 cgccgtactt ctttatgacc gcgtagggga aactcagcca gcggttgtcc tgctggactt
     1201 cgtcgatcag ccggaccact ttctgcaaaa aattctcgga ttttttagcc atagcactca
     1261 gtttccggcg ttccgccggt tttggcaagt aaaatttcca gctgacgcgc cgaagcagct
     1321 catcgctggc gcagggccat atcgtacagt tcgctggtgt agccggcttc gtcccggacg
     1381 cgcgcttcgt aggtctgttt ggcgacctgg ctgtgcgcga atacctgccg cagtatgcgg
     1441 gcgaccgctt cctgtcccgc cgtgtcgcca tccttttggg ctgtcttgaa ctgcacggcc
     1501 aactggtaga ggtcattgac cgggacccag aagtcgcgca agtcgtcgcc gtcatgttcg
     1561 gcaacccagg catcgattcc gtctttgccg taaatgaccg ccttgagagt ccggcggttg
     1621 ccgtccataa tcttgaggcc gagttccggg tctatctgca cgatgatcgg ataggtatcc
     1681 tgcgccgggt cggtgctgtg ttcgtcaatg agctttttct gcgtcgctct ggcttccgga
     1741 ttccgattca gcaggtccgc gatttccgag aagtgcatcc cggtgcgcag gtcgatgtcc
     1801 gggatccgcc gcagctggtc caggggagat gtcaggataa gttcgcgcgg gtcaaggtgt
     1861 tgcttggacc agcggacgtt ctcggcggtg agccagcggg tgaaatccgg cttgagcttg
     1921 atgctttcca tgaaggccgc gaagctgtcc cagaaatcgc gcggtccacc gtcctccagg
     1981 tgatatttga ccggctgctc ggcccagccg cgcaccgaat cgtccatggc gcgccagctg
     2041 tcgtccgcta ggcccaggaa cagcttgcgc agctctttgc cgagtatggt gcgcgccagc
     2101 tcaaggcgtt ccggcgccac gatatgctca ggataaactt cagggtttgt gggcataggc
     2161 aactcctgtg aaagatgctg gtacccccgg taggattcga acctacgacc tcaggcttag
     2221 aagtcctttg ctctatccag ctgagctacg ggggctcgtc ctctcgtacg cctttgcctc
     2281 gttttgccgg ggcggcaaaa gctgcggcct attgctacgg tcgtcctccc aaaagcccga
     2341 acggtcgact ccgctctctg gttcggtctt ttgtgtacgt ggtgcgggtg gggggaatcg
     2401 aacccccgtc accagtttgg aaaactggga taatagccgt tatacgacac ccgccgaatt
     2461 gttcggtctt gttcaggaat aacaggggta attatagcat aacgcctacc atcaagcgtg
     2521 gcatgctact tttcatgccc tcaagctaga gtaaggcata cgttatctaa cgagatcttt
     2581 taagattaca ccgtgttgtg tccagtaatg ccaacgggtc aatgtgaaag cgatagctgc
     2641 atagattata aatgccgcac tgcttaggtt aagcgatcta tttaaagtac gggtgaacaa
     2701 accgacattc atcaggtcat atcgaaggaa gccgaaactg cgttgctgcc atgcgatttt
     2761 gaagtatcgc ataaacccaa ggggcaggat gatcattgat tgcacgaacg aataaaaagg
     2821 caacagcaga tagacgggtc gccacttatg cttgcccgta ataagcagga gggtatagaa
     2881 ccaggacagt actatgaggg caagtaaggt gaaaggaaaa ttaattaatt ccacccagcg
     2941 tagcggcagc aacaaataaa tcagcaaaga gttataaaac agcataaaga agtggtacca
     3001 gccgtagctg gcgatattga ttacgtgatg cctcactccg cccgcaaacc aaatgattcg
     3061 ttggttaaac caaccgcgga cagtatcggg ggcggcagtg tagaaggtga aacgtatgtg
     3121 cttcagctta aagcccatga cttgcgctag cttaccgatt tctatatctc cgcctgcaaa
     3181 gaaatgtgaa tgatggctga aaaccttttg gaacgtgccg gcttttgcaa ggttgcaggc
     3241 gccggaggtt aaccacgggt ccat
//
LOCUS       DASSFD010000085         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1444262, whole genome shotgun sequence.
ACCESSION   DASSFD010000085 DASSFD010000000
VERSION     DASSFD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444262"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1233
                     /locus_tag="VFH39_01955"
     CDS             85..1233
                     /locus_tag="VFH39_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET6924570.1"
                     /translation="MPATKPIYLDHAATTPLDSRVLAAMQPYLTTQFYNPSATYLAGR
                     AVRRAVESARAGVAHWLGARPSEITFTAGGTEANNLAVHGIMAHYPGANLVTTTIEHD
                     AVLAPAAVWPHKLAEVGPDGVVTVEAVERLIDDQTVLVSVMYANNEIGTIQPLHRIAR
                     MLEAKRKERRASGNELPLLLHSDACQAALYLDLHAVRLGVDLLTINASKMYGPKQIGA
                     LYVKAGIVLEPVLYGGGQERGLRSGTENVAGIIGLAAALELAQTDRRQESDRLSNLQR
                     LFVAELEQAVPRVVINGSRKHRLPNNIHLTIPAADNERLMMALDEAGIMVAVGSACSA
                     SSGEPSHVLRAIGMTDEDARASLRLTMGHSTDEAAVRRTVAALAALVA"
     gene            1283..2131
                     /locus_tag="VFH39_01960"
     CDS             1283..2131
                     /locus_tag="VFH39_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924571.1"
                     /translation="MTKIKIKRLFKLPRVLAGFGLAFLVFASLQHPLSAQTVTQGYSS
                     DQPLQRGLIVQIKKSDATKVEPVSQDTIDQMHGVIVDANDAPVTLSTDSQKVFVATVG
                     HFAVLVSNQNGPIASGDYITVSAIDGIGMKAGTTEPVVVGRALDGFDGKTGAISSTQV
                     KDSSGNTRNVNIGRIQVDIGVARNPLLKAKAPDLPEFLQRASEAIAGKPVNAVRVYVG
                     LIIFIISTVIAGSLLYGGVRSAIISIGRNPLSRKSIIRGMLQVILTGIIIFISGILAV
                     YLLLRI"
     gene            2211..>2436
                     /locus_tag="VFH39_01965"
     CDS             2211..>2436
                     /locus_tag="VFH39_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924572.1"
                     /translation="MVIWALLHGSVPKAKKAAASSQDAYTQQAIQDVEHIFNDEFREE
                     LRNRGRLHFEKIIGENAMFLQQDLRLTTSQL"
BASE COUNT          540 a          659 c          731 g          506 t
ORIGIN      
        1 cgatgcgatc acacagtatg ggcagcgcgt ccagcgcgag ctctatgcag cggtcgctta
       61 agcaccgtac aatacactct gactatgccc gcaaccaagc cgatttatct tgaccacgcc
      121 gccactacgc cgcttgattc gcgggtgctg gcggcaatgc agccatacct gacgacccag
      181 ttttacaatc cgtcggcaac ctacttggcc ggccgggcgg ttcgccgggc ggtagagtcg
      241 gcccgggctg gtgtcgctca ttggctggga gcccgcccca gtgaaattac ctttacggcc
      301 ggcggtaccg aggctaataa cctagccgtt cacggtatca tggcacacta tccgggcgcc
      361 aatttggtga cgacgacgat cgaacatgat gccgtgctgg caccggcggc ggtatggccg
      421 cacaaacttg cagaagtcgg gccggacggg gtggtaacgg tcgaggcagt ggagcgtttg
      481 atcgacgacc agacggtgtt agtgagtgtg atgtatgcca ataacgagat cggcacgata
      541 cagccgttgc atcgtatcgc ccggatgctt gaagcaaaac gtaaagagcg gcgggctagc
      601 ggtaatgagc tgccattgct gctgcattcc gacgcctgcc aggcggcgtt ataccttgac
      661 ctgcatgcgg tccggctcgg ggtcgacctg ctgacgatta atgccagcaa gatgtacggg
      721 ccgaaacaga tcggagccct ctatgtgaag gcaggaatag tgctcgaacc ggtgctgtac
      781 ggcggtggcc aggaacgggg cctgcgcagc ggtacggaga atgtggcggg gattatcggc
      841 ttggcggcgg cgttggagtt ggcccagacg gaccgacgac aggaaagcga ccggctgagc
      901 aatctgcaac ggctcttcgt agccgaactg gaacaggctg tcccgcgggt ggttatcaac
      961 ggatcacgca agcaccggct accgaacaat atccatctga cgataccggc tgccgacaat
     1021 gaacggctga tgatggcact ggacgaggcc ggcatcatgg tggcggtcgg ttcggcttgc
     1081 agcgccagca gcggagagcc gtcacatgtg ctgcgggcga tcggtatgac cgatgaggat
     1141 gcccgggcaa gcctgcgcct taccatggga cacagcactg atgaagcggc ggtgcgccgt
     1201 actgtcgccg cgcttgcagc cttggttgct taatttgcaa caagtataac tgacagctat
     1261 actgattacg gttattcacg acatgacgaa gataaaaatc aaacgcttgt ttaagctacc
     1321 gcgcgtcctg gccggttttg gactggcttt tttggtattt gccagcctgc aacacccctt
     1381 gagtgcccag acagtgaccc aaggttacag cagtgaccag ccgttgcaac gcggcctgat
     1441 cgttcagatc aagaaaagcg atgcgaccaa ggtcgagccg gtcagtcagg atacgatcga
     1501 ccagatgcat ggtgtgattg tcgacgccaa tgatgcaccc gtcaccctgt cgacagattc
     1561 ccaaaaagtg tttgtcgcca cggtcggcca ctttgcggta ctggtgagca accagaacgg
     1621 accgattgct tccggtgatt acattaccgt gtcggcgatc gacggcattg gcatgaaagc
     1681 cggtactacc gaaccggttg tggtcggccg cgcacttgac ggctttgatg gcaagaccgg
     1741 tgctatcagc agtacacagg ttaaagacag cagcggtaac acccgcaacg tcaacatcgg
     1801 ccgcattcag gtcgatatcg gtgtggcccg caatccgctg cttaaagcta aggcgccgga
     1861 tctgccggag tttttgcagc gcgccagcga ggcgatcgct ggaaagccgg tcaatgcggt
     1921 ccgggtgtat gttggcctga ttatctttat catcagtacc gtcatcgccg gtagcctgtt
     1981 atatggcggt gtccgcagtg cgatcatttc gatcggtcgt aacccgctca gccgcaaatc
     2041 aatcatccgc ggcatgctcc aggtgatatt aaccggtatt attattttca taagcggtat
     2101 tcttgcggta tatttactac taagaatcta gtacatacaa aaaaggtggg acaaacgtga
     2161 cgctgtgggg gttaataatc atcttggtca gtatcggagc aggtaccggt atggttattt
     2221 gggccctgct gcacggcagt gtgcctaagg ctaagaaggc tgccgctagt agccaggatg
     2281 cctataccca gcaggccatt caagacgtcg agcatatctt taacgatgag ttccgcgagg
     2341 aactgcgcaa ccgcggccga ctgcacttcg agaagattat cggcgagaac gccatgttcc
     2401 tgcagcagga cctgcggctg actacctccc agctca
//
LOCUS       DASSFD010000086         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_935081, whole genome shotgun sequence.
ACCESSION   DASSFD010000086 DASSFD010000000
VERSION     DASSFD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935081"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1226)
                     /locus_tag="VFH39_01970"
     CDS             complement(30..1226)
                     /locus_tag="VFH39_01970"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HET6924573.1"
                     /translation="MTKRHRRSMGENGFAAIIISIVLVVVLSLITVGFASLMRKEQRS
                     ALDRQLSDAAYYAAESGINDAAAAVRAGFTATKDTCDPYTPATIAALPGNNNKNAAGK
                     YLVSSTVDNKSNTSYTCLLINPNPATLEYSSVNTGEATTAVLTGSSAAGTTTNVNTLV
                     FSWQDANGGQTFGPPTSGGLCPPVGANAFPPVSCWKNGGSAMTGILRISLTPVPHPAA
                     FSRSTLETTNYTAYLYPNGGGATSTPTTLSGSVVPSGVPSKPYAAGVTGSQNGTIITG
                     ACNTGSTPRYCAAAVTGLNTDTYLLHLSSIYAATQVTVTPYSCVGSNCTRIYLKGAQT
                     LIDSTGKSQDVLKRIQVRMPSRNSFDYPEFSIATGGPVCKQLTTWPQNQTTGTPGDTV
                     NLCDPL"
     gene            complement(1242..2003)
                     /locus_tag="VFH39_01975"
     CDS             complement(1242..2003)
                     /locus_tag="VFH39_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6924574.1"
                     /translation="MATKHTLKTEQGFTIVELMIALSVLAVLLVMSTVLLIQISKMYN
                     KGVTMSAIQNTDRNIVNDVASALEFGSAAPQFGASYANIGGTPVLVRSLCLDTKRYSY
                     IVGYQVADSPDTTLAPPQNYHALWEDTMQNAGQCFPLDITATSPTTSLDPNGVPTKAG
                     SGQELVPVRSRLTAFSVVQQSDGNGGYIYDVTANLAYGDNDLLCDSGAVGDCSSTATS
                     THLTSPSGSILCKGSAGQEYCATSHLSTSVTRRLK"
     gene            complement(2011..>2482)
                     /locus_tag="VFH39_01980"
     CDS             complement(2011..>2482)
                     /locus_tag="VFH39_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924575.1"
                     /translation="EVLIALAILGSAFGIAYATANKGLTAARNSEEHSQALQYINQQI
                     ELLRSAVHNTDAATAGVYSMTGKAFCLKEASPVQVVPFTSGWIPANEPADTDVQNAST
                     NYRPECSKDGLYYYSITYTPGTNEDIFQVLVRWQGIGMLGPQQESSSYKMHAVN"
BASE COUNT          526 a          660 c          710 g          586 t
ORIGIN      
        1 gcttccacaa taaggctaag tagcgccggc tataaggggt cgcagaggtt gaccgtatca
       61 ccgggagtac cggtagtttg gttttgcggc caggtggtca gttgcttaca taccggtccg
      121 ccggtggcta tgctgaactc cgggtaatcg aacgagttgc gcgacggcat acgcacctgt
      181 atacgcttca acacgtcctg cgactttccg gtggagtcta taagagtctg ggcgcccttt
      241 agatatatac gcgtacaatt tgagcccaca cagctgtagg gcgtaaccgt aacctgcgtg
      301 gcggcgtaga tagagcttag gtgcaataga taggtgtccg tatttaaccc cgtgaccgcc
      361 gctgcgcagt agcggggtgt actgcccgta ttacaggcgc cggtgatgat agtgccgttc
      421 tggctgccgg taacgcctgc cgcatacggc ttactcggga cgccgctcgg cactacgctg
      481 ccggacagcg tcgtcggcgt actggtggca ccgccgccgt tggggtacag gtaggccgtg
      541 tagttagtgg tttcaagggt ggagcgggag aaggctgccg gatgaggaac cggcgtcagg
      601 ctgatgcgca gtataccagt catagcactg ccgccgtttt tccagcaaga tacaggcgga
      661 aaggcgttag cgcccaccgg cggacaaaga ccgccgctag tcggcggtcc aaacgtctgc
      721 cccccattgg cgtcctgcca gctaaagacc agcgtattga cattcgttgt agtgcccgca
      781 gcgctggagc cggtgagaac ggctgtggtc gcttcaccgg tatttacgga actgtattca
      841 agggtggccg ggttaggatt gataagcagg caggtgtatg acgtattgga tttattgtct
      901 accgtggagc tgaccaggta tttgccggct gcatttttgt tgttattgcc cggcaaggca
      961 gcaatggtgg ccggcgtata agggtcgcaa gtgtcttttg tagccgtgaa gccggcccgc
     1021 acggccgcgg cggcatcatt tatccccgat tcggctgcgt agtaagccgc atcgctcagc
     1081 tgccggtcaa gtgccgagcg ctgctccttg cgcataagcg atgcgaaacc tacggttata
     1141 aggctaagca ccaccaccaa gacgatggag ataatgatag cagcaaaccc attttccccc
     1201 atactccttc ggtgacgttt tgtcataagc attattgtaa cctacttcaa tcttcttgta
     1261 accgatgtac ttaaatggct ggtggcgcag tattcctggc ctgcactgcc cttacataga
     1321 atgctgccgg aagggctggt cagatgagta cttgtagcgg tggagctgca atcgccgact
     1381 gcaccgctgt cacatagtag atcattatcg ccatatgcca ggttagccgt cacatcatat
     1441 atgtaaccgc cgttaccatc cgactgttga accacgctaa atgccgtaag ccggctgcgc
     1501 accggcacca gctcctgtcc gctgcccgcc ttcgtaggga cgccgttagg gtcaagcgag
     1561 gtagtgggcg atgttgccgt tatatccagg gggaagcact gccccgcatt ctgcatggtg
     1621 tcttcccaga gcgcatgata gttctgagga ggagccaaag tggtatcggg gctgtcagct
     1681 acctggtagc ccactatata gctgtagcgc ttggtatcaa ggcacaggct acgcaccagc
     1741 accggcgtac cgccaatgtt cgcatagcta gcaccgaatt gaggggctgc gctaccgaat
     1801 tccagagccg aggcaacatc gtttacgata ttccggtcag tgttctgaat ggcggacata
     1861 gtaacaccct tgttgtacat cttgctgatt tgtataagca gcacggtact catgaccaac
     1921 agcaccgcca gcaccgacaa cgcaatcatc aactctacta tggtgaaccc ttgttccgtt
     1981 ttgagtgtat gcttagtggc catgttcatc ctaatttacg gcgtgcatct tatagcttga
     2041 actttcctgc tgcgggccca gcataccgat gccctgccag cggaccaaca cttgaaaaat
     2101 gtcctcgtta gtacccggcg tatacgtaat gctgtagtag taaaggccgt ccttgctgca
     2161 ttcagggcga taattcgtgg aggcattctg tacatccgta tcggcaggct cattcgccgg
     2221 tatccaaccg ctcgtaaatg gcactacctg gacgggcgaa gcttctttga gacaaaaagc
     2281 cttaccggtc atgctgtata ctccggcagt tgccgcgtcg gtattgtgca ctgccgaacg
     2341 gagcagttct atttgctggt taatgtactg taaggcttgg gagtgctctt cggaattgcg
     2401 ggcggccgtc agacccttgt tggcggttgc ataggcgatg ccgaaggcgc tgcctagtat
     2461 agccaaagca atgagcactt ca
//
LOCUS       DASSFD010000087         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1055991, whole genome shotgun sequence.
ACCESSION   DASSFD010000087 DASSFD010000000
VERSION     DASSFD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055991"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFH39_01985"
     CDS             complement(<1..934)
                     /locus_tag="VFH39_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924576.1"
                     /translation="MNGIAARPLTRSGAALRTVAVGAGIVVAGVGAALTYHLYENAVH
                     QSITLVWYDWFDTNVHRWLVLPLTLVLTLMFFGLQHYLDPKSETHEEQGLGEMPAPSV
                     WHYVRVLLIGYFSLLAGAVLGPEAILVPACTLLGAYVGSRAFADQRITRLLAAAGFMA
                     LFTAFFDSFLVGVLSMALVMKQARVRFEPVLLLTAVVASASSYVTLQLVEGHALARLP
                     AYSWVLSFKDVLLCLVLVIAGYGMTAGIAGAHQVFGRIGRIARGTNWWWHALVAAAGI
                     AALYLIGGNLVEFTGNASIVPMFKQAAHLGFAGLI"
     gene            complement(931..1707)
                     /locus_tag="VFH39_01990"
     CDS             complement(931..1707)
                     /locus_tag="VFH39_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924577.1"
                     /translation="MAYRNREQYAAVIGKRRWLQSEGAYSVPDEAGSRVAILVPFLDE
                     FVQDERDSRANFERQTAVLMDRYTSQGRDPYHVMGATPEDFEQTLADRSVPTVLVAAF
                     GNFSAVVVPLSKDRGKDARYGYLDWLHLAGMATHLKLGKFVMLQCGSFDRQFNPPLGS
                     GVVSSYANIWGAAGRLRYTTDAEGIDAPIEPVTTEDELSYDEVKRAFPLQRNREVSGS
                     VPDPAYVAVRGVYNRFLNRNMPDIPRPEPIPHPDLRRYRS"
     gene            complement(1784..2185)
                     /locus_tag="VFH39_01995"
     CDS             complement(1784..2185)
                     /locus_tag="VFH39_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924578.1"
                     /translation="MRLKPFTPELYRRRVFSQLWMLALQFVLGMIMNLMGEQHRGTSH
                     VVYVVVLVTHILNAIGLAEGGVYIALKAPGKRNWVAAIVVLTTLTSGILTVRTRNDMW
                     SFVMACGFLASAWLYVMTYIRADRMVRAAQQ"
     gene            2233..>2754
                     /locus_tag="VFH39_02000"
     CDS             2233..>2754
                     /locus_tag="VFH39_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6924579.1"
                     /translation="MTIVRPYPRAKKDKEMSETLQHLILVRHGESEGDVRRAAFKRGE
                     PYQSTKTPQDEEITAHGIAECHSTGLWIKKHIIQAYGLRGFDGCYVSSAIRTEQSAVA
                     LELPIAVWQEDHDLDERNRGLVAGLRAEQHKDSFPKSFAQMRRDPLHWTPPNGEAIMP
                     GVTGRVRHFMDDIG"
BASE COUNT          632 a          876 c          744 g          502 t
ORIGIN      
        1 ctatcagccc tgcaaacccc aggtgagcag cctgcttgaa catcggtacg attgacgcat
       61 tacccgtgaa ttccaccaaa ttaccaccta tcaggtacag agcggctatg ccggcagctg
      121 ccaccagcgc atgccaccac cagtttgtcc cacgcgcaat acggccgatg cggccgaaga
      181 cctggtgggc gcccgctatt ccagccgtca tgccatagcc ggcgattacc agcaccaggc
      241 agagcaaaac atctttgaag ctcagcaccc aggaatatgc tggcagccgg gccaaagcgt
      301 ggccctccac caactgcaac gtcacatacg acgacgccga ggcgaccacc gccgtgagca
      361 gcagcaccgg ctcgaaacgc accctggctt gcttcatgac cagcgccata gaaagcactc
      421 ccaccaggaa cgaatcgaag aatgccgtga acagcgccat gaagccggcc gccgccagca
      481 agcgggtaat ccgctgatcc gcaaacgccc ggctacccac ataagcaccc agtaaggtgc
      541 aggccggcac caggatagcc tcgggcccga gcacagcgcc agcgagcagt gaaaagtaac
      601 cgatcaacag cacccgcaca taatgccaga cgctcggcgc cggcatttct cccagcccct
      661 gctcttcgtg ggtttccgat tttggatcga gataatgctg cagtccgaag aacattaacg
      721 tcagcaccag ggtaagcggc aatacgagcc accggtgcac attcgtgtcg aaccagtcat
      781 accacaccag cgtgattgac tggtgtacag cgttttcgta caggtggtag gtcagcgccg
      841 cccccacgcc agcaaccacg atacccgctc cgaccgcgac agttctgagg gcagcgccgc
      901 tccgggtcag aggccgtgct gctataccgt tcatgaccgg taccgccgta aatctgggtg
      961 cggtatgggt tccggccgcg gaatatcggg catattgcgg ttcaaaaaac ggttgtacac
     1021 gccgcgcacc gccacatagg ccgggtccgg caccgatccc gatacttcgc ggttccgctg
     1081 caaggggaaa gcccgcttga cctcgtcgta gctgagctcg tcttcggtgg tcactggctc
     1141 tatcggcgcg tcgatccctt ccgcatcggt cgtgtacctc aggcgcccgg cggcgcccca
     1201 gatattcgca tagctgctaa cgacgccgct accgagcggc ggattgaatt gccggtcgaa
     1261 gctgccgcat tgcaacatca cgaacttgcc gagcttcaag tgcgtcgcca taccggccaa
     1321 atgcagccag tccagatacc cgtaacgcgc atctttgccc ctatctttgg atagcggtac
     1381 gactaccgcc gagaagttgc cgaaagccgc caccaggacc gtcggaaccg accgatcggc
     1441 cagtgtctgt tcgaaatctt caggcgtcgc gcccatgaca tgatatgggt cgcgcccctg
     1501 ggaggtgtag cggtccataa gcaccgccgt ctgtcgttcg aaattagccc ggctatcccg
     1561 ttcatcttgc acgaactcgt ccaggaacgg caccaaaatg gcgacccgcg aaccggcttc
     1621 gtccggcaca ctgtacgctc cttcggactg caaccatcga cgcttgccga tgacggcggc
     1681 atactgctcc ctgttcctat atgccatgag ctcattgtaa gcacttatgg cctataccgg
     1741 ctgtgagcga agtccgccca cgttcttgga ggaagccagc gttttattgc tgggctgcgc
     1801 gcaccatacg atcggcacgg atgtaggtca tcacatacag ccatgccgag gccaaaaagc
     1861 cgcaggccat tacgaatgac cacatgtcat ttcgggtacg caccgtcaaa atgcctgagg
     1921 tcagggtagt taacaccact atcgcggcca cccagttgcg tttaccaggc gctttaagcg
     1981 caatatacac ccctccttcc gccaaaccaa tcgcatttag gatgtgggtg acgagcacca
     2041 ctacatacac tacgtgggac gtgcccctat gctgttcgcc catgaggttc atgatcatgc
     2101 cgagcacgaa ttgcagcgcc agcatccaca gctgcgaaaa tacccggcgg cgatacagtt
     2161 cgggagtgaa gggtttgagc ctcatgacta tcagtctacc gtaccatgtc tttcacatga
     2221 tacacttttc atatgactat cgtccgccct tacccgcggg cgaagaaaga taaagagatg
     2281 agcgaaaccc tacagcacct tatattggtc cggcacggcg agagcgaggg tgatgtgcgt
     2341 cgcgccgcct tcaaacgcgg tgagccttac cagtcgacaa agacgcctca ggacgaagag
     2401 ataaccgccc acggcatagc agaatgccac agcaccggac tctggatcaa gaagcatatc
     2461 atccaggcgt acgggttgag gggcttcgac ggctgctacg tctcatcggc catccggacc
     2521 gagcaatcag ccgtggcact ggaacttccc atagccgtct ggcaggaaga ccatgatctg
     2581 gatgagcgga acaggggcct agtagcgggt ctacgggccg agcagcacaa agattcattt
     2641 cccaagagct tcgcgcagat gcgccgcgac ccgctgcatt ggacgccccc gaacggcgaa
     2701 gccatcatgc ccggcgtcac aggtcgtgtg cggcatttca tggatgacat agga
//
LOCUS       DASSFD010000088         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_128999, whole genome shotgun sequence.
ACCESSION   DASSFD010000088 DASSFD010000000
VERSION     DASSFD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128999"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFH39_02005"
     CDS             <1..269
                     /locus_tag="VFH39_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924580.1"
                     /translation="AISGQAFNTASNQREKDAPLIKQLPYIERYFRIDYGPSKAHPGD
                     DTAVAFYITTYGQPGVQQALDWIKAQGYNPDTLEIVYTDQSSGQ"
     gene            complement(273..1334)
                     /gene="recF"
                     /locus_tag="VFH39_02010"
     CDS             complement(273..1334)
                     /gene="recF"
                     /locus_tag="VFH39_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET6924581.1"
                     /translation="MISEVRLQHFRSYTDARYSFSPQVNIIVGPNASGKTNLLEAVLV
                     LARGSSYRAKDGDLIAFGEPWARLDALADEEARTVKLVAEPLPMKSYDIGGKPFKRLS
                     LQHSLPVVLFEPNHLQLLHGSPDQRRNYLDDLLEQLTPGYSGYRRNYRRVLSHRNALL
                     KRSRLPSNEELFPWNLRLSELGAVMSRARAALVARANEQLDGLYKDMSGVTTTVAMKY
                     HHHFPLETYESRLLHRLESELSTDLMRGFTAAGPHREDFELYFNDTPADETASRGETR
                     TAILALKVIELQELATARGQTPLLLLDDVFSELDASRRAALTAHLQPYQTFITTTDAD
                     VTARVFKKHATTITLGAAL"
     gene            complement(1347..2438)
                     /gene="dnaN"
                     /locus_tag="VFH39_02015"
     CDS             complement(1347..2438)
                     /gene="dnaN"
                     /locus_tag="VFH39_02015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET6924582.1"
                     /translation="MKLQVTQENLNKALGTVARVANARGTLPILANVLLKTSNNRLSL
                     AATNLDIAITQYIGSKVSEEGAITVPARLMQDFVASLPSGVIDLELTDTKLHITTDQY
                     QSTINGIMADDFPVMPAIAKGETWSVDAAELKKGLQQVVFAASNDESRPVLTGCLIHS
                     VDGKLLLAATDSYRLAEKRIGATKADVRMLVPATALQDLLRIIGDHEGEVHVTHDDQQ
                     VLFTVGEVELVARLLEGNYPDYAKLIPESFAVEARLKRADFINVTKVSSLFARESAGS
                     VTLKVDSKSQSLSIRAVASQLGENTATAAGTITGDGEITLNSRYLLDALQAMSGDEVV
                     FGFNGKLEATMLRENGDSDYTHIIMPLKS"
     gene            complement(2712..>3233)
                     /locus_tag="VFH39_02020"
     CDS             complement(2712..>3233)
                     /locus_tag="VFH39_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6924583.1"
                     /translation="MQIPDFETRCAIVQTKAQNHDTELPPDVVEYLANNIQTNIRELE
                     GALNQLLAFCEMRALEPNLSIATSLLGINRGRPKHLSAKQIIERTARYFQVPLEDLLG
                     PKRDKDIVVPRQIAMYMLRSELHLSFPKIARELGRKDHTTAIHSIEKIEKEARSDVDI
                     RSAISEIKDKLYA"
BASE COUNT          637 a          932 c          958 g          706 t
ORIGIN      
        1 gggcgatatc cggtcaggca tttaacaccg ccagcaacca gcgggaaaag gacgcaccgc
       61 tcatcaagca actgccctat attgagcgct atttccgaat tgactatggg ccgtccaagg
      121 ctcaccctgg cgatgacacg gcggtcgcct tctacataac aacgtacggg caaccgggag
      181 tacagcaggc gctcgattgg ataaaagccc aaggctataa ccccgataca ctggaaattg
      241 tctacaccga ccagtcttcg gggcaatagg aattaaaggg cggcaccgag cgtaatggtt
      301 gtcgcatgct tcttgaatac cctggcggta acatccgcgt cagtggtggt aatgaacgtc
      361 tggtagggct gcaggtgagc cgtcagggcg gcacggcggg aggcatcgag ttcactgaaa
      421 acatcgtcga gcaggagcag gggagtttgc ccgcgggctg tcgccagctc ctgtagttcg
      481 atgaccttga gggcgaggat ggcggtgcgg gtctcgccgc gcgaggccgt ctcgtcggct
      541 ggcgtgtcgt taaagtacag ctcaaaatcc tcgcggtgcg gacccgctgc cgtgaaacca
      601 cgcatcaggt cggtcgacag ttccgactcc agccggtgca ataggcgcga ctcgtacgtc
      661 tcgaggggga aatggtggtg atatttcatg gccaccgtcg tggtcacgcc cgacatatcc
      721 ttgtacagcc cgtccagctg ctcattggcc cgtgccacca aggcggcgcg agcgcgggac
      781 ataaccgctc ccagctcgct caggcgcaaa ttccacggga ataactcttc gttactgggt
      841 agcctactgc gcttgagcag ggcattgcgg tggctcagca cccggcggta gttgcgccgg
      901 taaccgctat agccgggcgt cagctgctcc aggaggtcgt ccaggtagtt gcggcgctgg
      961 tcaggagaac cgtgtagcag ctgcaagtga ttcggctcaa acagcactac cggcagactg
     1021 tgttgcaaac tgaggcgctt gaacggcttg ccgccaatgt catagctctt catgggcagc
     1081 ggttcagcca ccagctttac ggtccgtgcc tcctcgtcag ccagagcatc gagccgggcc
     1141 cagggttcac cgaaggcaat cagatcgccg tccttcgccc ggtatgaact gccgcgcgcc
     1201 agcaccaaca cagcctccaa caggttggtc ttgccgctgg cattcggtcc gacaatgatg
     1261 ttcacctgcg gactgaaact gtaccgcgcg tcggtatacg accggaagtg ctgcagccgg
     1321 acctcagaaa tcattcctct attgtattac gacttcaggg gcatgatgat gtgggtgtag
     1381 tcgctgtcgc cgttttcgcg cagcatcgtg gcttccagct tgccgttgaa gccgaagacc
     1441 acttcgtcgc cgctcatcgc ctgcaaggcg tcgagcaggt agcgtgaatt aagcgtaatt
     1501 tcaccgtcgc ccgttatcgt tccggcagcc gtcgcggtat tctcgccgag ctgggaggcg
     1561 accgcccgga tactgagcga ctggctcttg ctgtccacct tgagcgtgac gctgccggcg
     1621 ctctcgcggg cgaacaggct cgataccttg gtgacattta tgaagtcggc tctcttcagt
     1681 cgcgcttcga ccgcaaagct ttccggaatc agtttggcgt agtcaggata attaccctcg
     1741 agcaggcgag ccaccagctc gacctcgccg acggtgaaca atacctgctg atcgtcgtgc
     1801 gtcacatgca cctcaccctc gtggtctccg atgatacgca gcaaatcctg cagagcagtg
     1861 gccggtacca acatccgtac gtccgccttg gtagcaccga tacgcttctc ggccaaccgg
     1921 tagctgtccg ttgccgccag cagcagtttg ccgtccacgc tgtggatcag gcagccggtc
     1981 agcaccgggc gcgactcgtc gttgctggcc gcgaacacca cttgctgcag acctttcttg
     2041 agctctgcgg cgtcaacgct ccaagtctcc cccttggcga tggccggcat gacagggaag
     2101 tcgtcggcca taataccgtt gatagtggat tggtactggt cggtggtgat gtgcagtttc
     2161 gtatcggtca gttccagatc gatgacgccg ctcggtaaac tggccacgaa gtcctgcatc
     2221 agccgggcag ggacggtgat ggcaccttct tcgctgactt tgctgccgat gtactgcgta
     2281 atggcgatgt ctaggttggt ggcggccagg ctcagccggt tgttcgaggt tttcagtagt
     2341 acgttcgcca ggataggcaa ggtaccgcgc gcattcgcca cccgggccac cgtaccgagt
     2401 gctttgttca ggttttcctg agtgacctgc agcttcatgc tataactcct aatttattaa
     2461 tatactttgt agttgtattt ataggccctg tggaagcagg ggataaccgg gaaaataaca
     2521 gcggtaaaag cgcatcggca acagtggata acgaagcgga aataatacca ctagcctggg
     2581 tacaaaataa gtacttttcc cctgcccgaa cattttgcga actagctgcc cccagcttac
     2641 gcacagtacg gcggcaggtt gtaccggctt cgtgcacacc gcttccacag gaccagtcca
     2701 taaaacggca cttaggcata gagcttgtcc ttgatctcgc tgatagcact gcggatgtcg
     2761 acgtcggagc gggcctcctt ctcgattttc tcaatcgagt ggatggcggt cgtgtggtcc
     2821 ttgcgaccca gttcgcgggc gatcttcgga aagctcaggt gtagctcgct cctaagcatg
     2881 tacatggcaa tctggcgcgg tacgacgatg tctttgtcgc gcttcggtcc gagtaaatct
     2941 tcgagcggca cctggaagta gcgtgccgtc cgctcgataa tctgcttggc actcaaatgc
     3001 ttcggccgtc cgcgattaat gccgagcagg ctggtggcga tgctgaggtt cggttccagc
     3061 gcccgcatct cacagaacgc cagcagctgg ttcagggcgc cttccagctc gcggatgttg
     3121 gtctggatgt tgttggccag gtactcgacc acatccgggg gcagttcagt atcgtggttc
     3181 tgagccttgg tctggacgat ggcgcagcgg gtctcgaagt ccggaatctg cat
//
LOCUS       DASSFD010000089         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1692303, whole genome shotgun sequence.
ACCESSION   DASSFD010000089 DASSFD010000000
VERSION     DASSFD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692303"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..2411
                     /locus_tag="VFH39_02025"
     CDS             453..2411
                     /locus_tag="VFH39_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET6924584.1"
                     /translation="MKQQKPSIHYYSKPGGRSPASVVLGLAVALLVGASGAYLIHSSS
                     ANGGGGPLIYVATNPAGVANACWDVQLSWDNSNWADYGTRCTDSSGQYVMDSQYTYYR
                     ITATTVPAGYGFKQWCLPSGTCTSNQTIYFASSAYANQHIYMSLSAPPAPPSGPRLSV
                     SGSTGSSIGLVWSAGSSGSYGSISGYQIRRDNSYLTTAAAGSTGYTDTGLGCGQTHNY
                     SVTLVTSGGATASSNTVTGATSACPSPAPSGGGTSTGTASKPVSPVSAASGGAAQQPK
                     VVIAPAQPPSAPNGFAADVVSGKVIQLSWNAGLAGAGVQNYALDRSTDNVTWQPLATP
                     LGTSYTDTTADFDTHYYFRVREVDTDGATSDYATTEVTTGRFTSTAGSIQSDDRLVTA
                     AIPDGAFGQPVNCSITSVSDGPPSIPTRSLLAGPYSVLCVAADGSTVTGFAEPLRVTM
                     KLGSLAKGHDSFAVQLFGDDGTGSDAHAAFDARHNTLSFKLDSSKSFAAYGTKRASLW
                     PAVFKGLMLLLAFAGTVGVIVYLRRTRPGRPLPLAGHLSLPAAATDPSATAMFEQAVN
                     KPNCTHLNMARQVQPQSTGCAECIAEHTHWKSLRICLSCGHVGCGEDSSGQHARKHYE
                     QSGHPLIYEYGNPAGDAIGWCYIDQTYI"
     gene            complement(2920..2996)
                     /locus_tag="VFH39_02030"
     tRNA            complement(2920..2996)
                     /locus_tag="VFH39_02030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2960..2962),aa:Val,seq:gac)
BASE COUNT          702 a          920 c          974 g          681 t
ORIGIN      
        1 aagccccttg agtgtcctga accatgacaa catattgtca taacgctatt aaaaatgcta
       61 gtggaaaagc tgcggagtat tggggaaaac aaaacccggg cttagtcccg ggctttctaa
      121 catgcgactg tctaattgtt attggccgca gcggcggagg ttctccacct acggatacgt
      181 ttgtacgatg ctgtggaggt aatctcctca taaaacagct taaaatcgtc caaagcgctt
      241 gccttggtgc tactcactcg tagtccctgt ttaactgaat gattttagca gtaaaggtta
      301 tatcagtcaa taatgctagt aaaaaagcta gtgcttttcg ggcgtacatg ccgggttcta
      361 agccgtagcc gtatatccgg tgctatggta gagtatacgt aagctggtcg cgccttgggc
      421 tgcctgatca aacgagggcg cgtataacgt ccatgaaaca acaaaaacct tcgatacact
      481 actacagcaa gccgggaggc cgttcgcccg cctctgttgt gcttgggttg gcggtggcac
      541 tgttggtagg cgccagcgga gcctacctaa tccattcgtc ttcggcgaac gggggcgggg
      601 gccccttgat ttacgtggca accaacccgg ccggcgtcgc caacgcctgc tgggacgtgc
      661 agctgtcctg ggacaattcc aactgggctg attacggcac ccgctgtacc gacagcagcg
      721 gacaatacgt gatggacagc cagtatacct actaccggat aacggcaact actgtgccgg
      781 ccggctacgg cttcaagcag tggtgtctgc cgagcggtac ctgtaccagc aaccagacta
      841 tctacttcgc gagcagcgcc tacgccaatc agcacatcta tatgtctctt tctgcgccgc
      901 ccgcacctcc ctcggggcca cgcctgagcg tcagcggcag taccggctcc tctatcggtc
      961 tggtgtggtc ggccggatcg tcgggcagtt acggcagtat cagcggctac cagatacggc
     1021 gcgataacag ttatctgacg accgctgcgg ccggcagtac cggctatacc gatacgggcc
     1081 tggggtgcgg acagacccac aactattcag tgacgcttgt tacctctggc ggtgcaacgg
     1141 cttcctctaa caccgtgacg ggagcgacat cggcgtgccc gtctccggct ccttcggggg
     1201 gcggtacctc gaccggcacc gcttcaaagc cggttagccc ggtgtcggca gcatcgggcg
     1261 gggcggcaca gcagcccaag gtggtgatcg caccggcgca gccgcccagt gctccgaatg
     1321 gatttgcggc cgacgttgtc tcgggcaagg ttatccagct tagctggaac gccgggctgg
     1381 ccggcgccgg tgtacagaac tatgccctgg accgttccac cgacaacgtc acctggcagc
     1441 cgctggctac gcccttggga accagctaca cggataccac ggccgatttc gatacgcact
     1501 actatttccg ggtgcgcgag gtggacaccg acggggcaac ttcggactac gccaccacgg
     1561 aggtaaccac cggccgattt accagtacgg ccggcagcat ccagagtgac gataggctgg
     1621 taaccgcagc tataccggac ggcgccttcg gccagccggt gaactgcagc atcaccagtg
     1681 tcagcgacgg cccgccgagc atacctaccc ggtcgctgct ggcgggaccg tactccgtgc
     1741 tgtgcgtggc agcggacggt tctaccgtga ccggctttgc cgaaccgttg cgggtgacaa
     1801 tgaagcttgg cagcctggct aaaggtcatg attcgttcgc agtccagctg ttcggcgacg
     1861 acggcaccgg cagtgacgcg catgcggcat tcgatgccag gcataatacg ctgagtttca
     1921 agctggattc cagcaagtcg ttcgctgctt atgggaccaa gcgtgccagc ctctggccgg
     1981 ccgtgttcaa ggggttgatg ttgctgctgg cgttcgccgg caccgtgggc gttattgtat
     2041 acttgcggcg gacccggccc ggacggccac tgccgctcgc cggccatttg tccttgccgg
     2101 cggcagcgac ggatccgagc gctaccgcta tgtttgagca agccgtaaat aaaccgaact
     2161 gcacccacct gaacatggca cgacaggtac agccgcagtc gaccggctgc gccgagtgca
     2221 tcgccgaaca cacccactgg aaatcgctgc gtatctgcct cagttgcggg cacgtcggtt
     2281 gcggcgagga cagtagcggt caacatgccc gtaagcacta tgaacagagc ggccacccgc
     2341 tcatctatga gtacggtaat ccggcaggag atgctatcgg ctggtgctac attgatcaaa
     2401 catatattta gaattcagac tgcccggctt ggcgcacctc aggcgcagca gccaccaacc
     2461 cgtaagccgt gatgcgcggt attggagcga ggcgaggttg atctgagggg gtgcgggtcg
     2521 gacggttcaa ccggagctag gggttgccgg gggccgagac gcggtttacc ggtctgcacc
     2581 ggtatggctg ccgaggtgcc ggtaggcgaa gccggcagat ggcccggtgt atgaactgga
     2641 ttgtgcgggt gccgccagtt ggccgccggg atagcctggg ccaagaactc caagatgtcc
     2701 ccagtgatac ctggatctag acggctgcgg cgagggcagc gggagcgtgc agtaccgtta
     2761 gcgagcacct ggtggcgccg ttgcttacct catattgtac ccggctggtt atatccgtta
     2821 ctgcgcgtct ctccggggta gtgacaggtg gagtcatgtg cctattgcag agcggcaggg
     2881 ccgtgcgaaa ataaaaaacc tcgtcatagc aagggttatt ggtgggcgat agaggattcg
     2941 aacctctcac ctcttccacg tcaaggaagc gctctagcca aatgagctaa tcgccctcag
     3001 caaacgagaa ctggacaatt atagcattac aggcgggctt cgctaaaata aactcattta
     3061 tgatatggga agcgagttgt tcaataatat aaatatcatc catgatgtta agctgaagat
     3121 cttatccaca tggggaagtg taggaaatac agcataaata tactagccat atagtactaa
     3181 ccttgttata gtactttgca caggtgggtt ttgcgagcgg ttttccgctc acgaagcgaa
     3241 agaaggttga atgacattgc gagatcattc aaccttc
//
LOCUS       DASSFD010000090         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_266536, whole genome shotgun sequence.
ACCESSION   DASSFD010000090 DASSFD010000000
VERSION     DASSFD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266536"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFH39_02035"
     CDS             complement(<1..921)
                     /locus_tag="VFH39_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6924585.1"
                     /translation="MVGFIIFIIIVVVGAAIAGPIIFMNMRKRGREQKNYERGLKMVP
                     LLIHLPPPSDDTEVGGRDVRDVTDETISKAQIIYNIIDSTFQKGFKAKFYGQRHFSFE
                     IVGSGGFVYFYAIVPVALVDVVKQAVVSAYPAARLEEVTDHNIFNKVGKITGTAGGEL
                     KLKQSYAYPIATYQDLKRDAMQSVLNSFSTLEKEDGAGIQILLRPADSGWRKTASGLA
                     SKKRKGEDKGNGAGAFGKQLLSAAWKPPEGKEEGGKPPKQLSDLEQSTLNAIDEKTRH
                     PGYEVQIRIIASSNISQRAQAIVSNVVASFS"
     gene            complement(933..1337)
                     /locus_tag="VFH39_02040"
     CDS             complement(933..1337)
                     /locus_tag="VFH39_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924586.1"
                     /translation="MNPNVDTPPSMPMPEQFPTPPDGAAGAELAPVSAERAPQPVAVP
                     ERPPMPMVPVASTSVPMPQPANATAGEMPAVTGTPSVAADNDLIEKEGVLKAKQIVNA
                     TRQDPYQQNRQLAAIKADYMQKRYNKTVKLSE"
     gene            complement(1407..1763)
                     /locus_tag="VFH39_02045"
     CDS             complement(1407..1763)
                     /locus_tag="VFH39_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924587.1"
                     /translation="MKRHSRSLWQLAGLAVLDAAFFGFINPTNAYAVVVMIGFLLLVA
                     TLYVVVDLLLALAERIVTFRPTTRYRLRLSITAFLALLIAMQSIGQLTVKDMAAIIPL
                     VLVAAFYASYQRKQAR"
BASE COUNT          520 a          695 c          632 g          547 t
ORIGIN      
        1 tgaaaaactg gccacaacgt tgcttacgat ggcttgagca cgttgtgaaa tgttcgacga
       61 cgcgatgatt cgtatctgta cttcataccc cggatgccgg gttttctcgt cgatagcgtt
      121 gagtgtagac tgttctaaat ctgacaactg cttgggtggt tttccgccct cttccttgcc
      181 ttctgggggc ttccaagctg cagacaaaag ttgctttccg aacgcgcccg caccgttgcc
      241 tttgtcctcg cctttgcgct ttttgctggc caacccgctg gccgttttgc gccacccgga
      301 gtcggccgga cgcagcagaa tctggatgcc cgcgccgtcc tctttttcga gcgtgctgaa
      361 cgagttcagg accgactgca tggcgtcgcg tttcaggtcc tggtaggtcg cgatcgggta
      421 ggcgtaggac tgcttcagtt tcaattcgcc gcccgccgtg ccggtgatct tgccgacctt
      481 gttgaagata ttgtggtccg tcacttcctc caaccgggcg gccggataag cgctcaccac
      541 ggcctgtttc accacgtcca ccagcgccac cgggacgatg gcgtaaaagt acacgaagcc
      601 gccggagccc actatctcga aactgaagtg ccgttggccg tagaatttgg ccttgaagcc
      661 cttctggaac gtactgtcga tgatgttata gatgatctgc gccttgctga tggtctcgtc
      721 ggtcacatcg cggacgtcgc ggccccctac ttcggtgtca tcgctcggag gcggcaaatg
      781 gatgagcagc ggcaccatct tgagcccgcg ctcgtaattc ttctgctcgc gtccgcgctt
      841 acgcatattc atgaagatga tgggtccggc aatagccgcc ccgaccacca cgatgatgat
      901 gaagatgatg aagcctacca tttcctaacg gcctactcgc tcagctttac ggttttgtta
      961 tagcgcttct gcatgtaatc ggccttgatg gctgccagct ggcggttctg ctggtacgga
     1021 tcctggcggg tcgcgttcac gatctgcttg gccttcaaga ccccttcttt ctcaatcagg
     1081 tcgttgtccg cggccacgga cggtgtaccg gtcacggccg gcatctcgcc agccgtggca
     1141 ttggccggtt ggggcatcgg tacagaggta gacgcaaccg gtaccatcgg catgggaggt
     1201 cgctccggca cggctaccgg ctgcggggca cgctcggcac tcaccggggc cagctcggcg
     1261 ccggcagcac cgtccggagg cgtcgggaac tgctccggca taggcatact tggtggggta
     1321 tctacgttcg gattcataat ttagtcttta ttataccgct gaccgctgtt cgacaccaga
     1381 cttcgcggct ttccacccgg cggctgtcaa cgagcctgct tacgttggta cgaggcataa
     1441 aaggccgcca ccaacaccag aggtatgatg gccgccatat ctttcaccgt cagctgcccg
     1501 atggactgca tggcaataag caaggccaaa aaggccgtga tcgacaaccg cagccggtac
     1561 cgcgtcgtcg gccggaaggt aacgatgcgc tctgccagcg ccaacagcag atcgacgacc
     1621 acatacagcg tcgccaccag cagcaagaag ccgatcatca ctaccaccgc ataggcattg
     1681 gttgggttga tgaacccgaa gaaggccgca tcgagcacag ccaggccggc caattgccac
     1741 agactgcgcg agtgccgttt cataccccca tcataccaga tagccacaag cggttagcgg
     1801 gcccgtcggc ctatacaaaa agcaccgccc gtaaaggcag tgctcttgcg ctgttgaata
     1861 aggtgcctag gcgacaaatc cgctgctata aaagactatt gagcgcttgc cgccgtcggc
     1921 gtgactgacc gctcctcaca gcttgaggct gcttggagcg ttagacggct taagtatttc
     1981 gagagcgaaa ttttgtctta agccgttagg agctttttgt aagtttttgt tttagtaact
     2041 tacaggttta atcctaccgc agaaaccttg acatgtcaac gataatattg ccaaatcgat
     2101 gcagcccgta caacgattct gcgccgtttt gggtcgctgc gctggcccca catatgtggc
     2161 ttataaattc cgcactcacg ctaatcagcc gaccccgaaa agtttatgca caggaatggt
     2221 ccaaaattgt aaaaattaac cataaaagtt attgacagcc cacccgcgaa tccctatatt
     2281 taagcgtagc actgaataaa ttaagtgctc aaaaacaaaa aagccaatag aaacgacgac
     2341 ttctcgcaag tcgtcgttcc atcagaaacc aacaccttaa atctcaaaac caaa
//
LOCUS       DASSFD010000091         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1800889, whole genome shotgun sequence.
ACCESSION   DASSFD010000091 DASSFD010000000
VERSION     DASSFD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800889"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="gatB"
                     /locus_tag="VFH39_02050"
     CDS             complement(<1..1121)
                     /gene="gatB"
                     /locus_tag="VFH39_02050"
                     /inference="COORDINATES: protein motif:HMM:NF004014.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET6924588.1"
                     /translation="MIDPAVRDRYTVTIGIECHVQFKTRSKLFSGADNDARVAAPNTL
                     VNHIDFGLPGALPVLNREALRLACRAAFALGTTPQKFSKFDRKHYFYPDLPMGYQITQ
                     YDEPIILGGSVAIEVDGQPKTIGITRAHMEADAGKSTHPAGADYSLVDLNRAGTPLLE
                     IVSEPDMHTAAEAKAYAKELYLRMKYAGVSDANLYYGNMRFDVNVSVSKTAELGTRTE
                     TKNLNSFRSVEKAVEYEVNRQIEELEAGKAIVQETRGWDDAKQKTFSQRSKEDAQDYR
                     YMPDPDLPPIVLDDAFIAEVQAELPALPDDYRKILGELGIDAKTVSDIIAVPDTAMTV
                     KRVYEAADASHAKRVAFWLLQPQSDDTEETEAAEAKNVNA"
     gene            complement(1118..1558)
                     /locus_tag="VFH39_02055"
     CDS             complement(1118..1558)
                     /locus_tag="VFH39_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924589.1"
                     /translation="MNVLGFEFPTMTIVIVVAAIVLLLLIVFTARFTHRRPGKLNTAY
                     FTQRWYETQGLCASQNTWPLAVINADKLLDEALKKRKYKGQTMGERLVSAQHDISQND
                     SVWFAHKLRNKLVHEDFTKLKKKEVLQALMGFRNALKDLGALDK"
     gene            complement(1555..2982)
                     /gene="gatA"
                     /locus_tag="VFH39_02060"
     CDS             complement(1555..2982)
                     /gene="gatA"
                     /locus_tag="VFH39_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET6924590.1"
                     /translation="MVEWEPIATLAAKVNNGEVKAADLVERSLKAIADSADYDAIIAT
                     TEERARARAAAIDAKVAAGENAGRLAGVPFIAKDNFLAFGAETTAASNILKGFVAPYQ
                     ATAIQRLEGEGAILVAKANLDAFAHGGSTENSDFMVTKNPHDKTRVPGGSSGGSAAAV
                     VLGLAPFALGTDTGGSIRQPASFSGCVGYKPTYGLVSRSGVVAMASSTDVIGPLTMSV
                     ADAALVLDVMADRDPLDSTTIERDPAGYTTGETPDLRGKKIGVIKEYLAEGLKTGVME
                     TVDAAALTLKAAGAEIVEVSLPTLPVALAVYYILCPAEVSSNLARYDGQRYGYSDPSA
                     TTLEESYLLSRGKGFGREAKRRIMVGTYVLSSGYYDAYYRKAQVVRTKLIKEFEAAFQ
                     QVDYLLGPSAPSTAFKLGENVDDPLMMYLSDVMTVAANLTGIPAISLPCGESDGLPVG
                     LQLMAPQRRDRDLLALAKATEEVLA"
     gene            complement(2982..3275)
                     /gene="gatC"
                     /locus_tag="VFH39_02065"
     CDS             complement(2982..3275)
                     /gene="gatC"
                     /locus_tag="VFH39_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET6924591.1"
                     /translation="MANLSRDDVLKLASLARISLDDDEVEALSHDLTTILQYVEQLQT
                     IDVDGLEPTNQVSGLTNVMRDDAIIDYGYAPKDLLKNVPQVQDDLLKVKRMIG"
BASE COUNT          600 a         1000 c         1099 g          750 t
ORIGIN      
        1 gcattaacat ttttcgcctc tgcggcctct gtttcttcgg tgtcgtcaga ttggggctgc
       61 agcagccaga aggccacccg tttggcgtgt gacgcgtcag cagcctcgta gacgcgtttg
      121 acggtcatag cagtatccgg cacagctatg atgtcactga ctgtcttggc atcgatgccg
      181 agctcaccga gaatcttacg gtagtcgtcg ggcagtgccg gcaattcagc ctggacttca
      241 gcaatgaagg cgtcgtccag cacaatgggc ggcaggtcgg gatcgggcat gtagcggtaa
      301 tcctgggcgt cctctttgct gcgctggctg aacgtcttct gtttggcatc gtcccaaccg
      361 cgggtttcct ggacgatggc tttaccggct tcgagttctt cgatttggcg gtttacttcg
      421 tactcgacgg ccttttcgac gctgcggaag ctgttgaggt tcttggtctc ggtgcgggtg
      481 ccgagctcgg ccgtcttact cactgaaacg ttgacatcga agcgcatgtt gccgtagtac
      541 aggttggcgt cactgacgcc ggcgtacttc atgcgcaggt ataactcttt ggcgtaggcc
      601 ttggcttcgg cggcggtgtg catgtcgggc tcactgacga tttcgagcag cggcgtgccg
      661 gcgcggttca ggtcgaccag gctgtaatca gccccggccg ggtgggtgct tttgccggcg
      721 tcggcctcca tgtgggcgcg agtgatgccg atggtcttcg gctggccatc gacctctatg
      781 gcgaccgaac caccgaggat gatcggttcg tcgtactggg tgatctgata ccccatgggc
      841 aggtcggggt agaagtagtg cttacggtcg aatttgctga atttctgcgg cgtggtgccg
      901 agggcgaagg cggcccggca ggccaggcgc agcgcctcac ggttcagcac cggcagcgcg
      961 ccgggcagac cgaagtcgat gtggttgacc agggtgttcg gggcggccac gcgggcatcg
     1021 ttatcggcgc cgctgaacag tttgctgcgc gtcttgaact gcacgtgaca ctcgataccg
     1081 atggtcacgg tgtagcggtc gcggacagcc gggtcgatca tttgtcgagc gctcctaagt
     1141 ctttgagagc gttgcggaag cccataaggg cctgcagcac ctcttttttc ttcagcttgg
     1201 tgaagtcttc gtggaccagc ttgttgcgca gcttgtgcgc gaaccagacg ctgtcgttct
     1261 gggaaatgtc gtgctgggcc gacaccaggc gctcgcccat ggtctgaccc ttgtacttgc
     1321 gcttcttgag cgcttcgtcg agcagtttgt cggcgttgat gacggccagc ggccaggtgt
     1381 tctggctggc acagaggcct tgggtctcat accagcgctg ggtgaagtag gcggtgttca
     1441 gcttgccggg gcggcggtgc gtgaaccggg cggtgaagac gatgagcaac agcaagacga
     1501 tcgccgccac cacgattacg atagtcatcg tggggaactc gaatcccaag acgttcatgc
     1561 caacacctcc tcagttgctt tcgctaacgc taataaatcg cggtcccgcc gctgcggtgc
     1621 catcagctgc agcccgaccg gcagaccgtc gctctcgccg caaggcagac tgatggccgg
     1681 tatgccggta aggttggccg ccacggtcat gacatcgctc aagtacatca tcagcgggtc
     1741 atccacattc tcgccgagtt tgaaggcggt gctgggggcc gacggtccca acaggtagtc
     1801 gacctgctgg aaggcggctt cgaactcttt gatgagcttg gtgcgcacca cctgcgcctt
     1861 acggtagtag gcgtcgtagt agccgctgct cagcacgtag gtgccgacca tgatgcggcg
     1921 cttggcctca cggccaaagc ccttgccgcg cgacaacagg tagctttctt cgagggttgt
     1981 ggcggacggg tcgctgtagc cgtaacgttg gccgtcgtag cgggccaggt tgctgctgac
     2041 ttcggccgga cagaggatgt aatacaccgc cagcgcgacc ggcagggttg gcaggctgac
     2101 ttcgacgatt tcggcacccg ccgctttgag cgtcagggcg gccgcgtcga cggtttccat
     2161 gacgccggtc ttcaggcctt cggccaggta ttctttgata acgccgatct tcttgccccg
     2221 cagatcaggc gtttcaccgg tcgtatagcc ggccggatcg cgctcgatgg tcgtgctgtc
     2281 caaggggtcg cggtcggcca tgacatcgag caccagtgcg gcatccgcca cgctcatagt
     2341 gagcggaccg atgacatcgg tgctgctggc catggcgacg acgccgctgc ggctgaccag
     2401 gccgtaggtc ggcttgtagc cgacacagcc gctgaagctg gccggctggc gaatggaacc
     2461 gccagtgtcg gtgccgagcg caaacggcgc cagaccgagc accacggccg cggccgaacc
     2521 accggaactg ccgccgggca cccgggtttt gtcgtgaggg ttcttggtga ccatgaagtc
     2581 gctattttcg gtgctgccgc cgtgggcaaa agcgtccagg ttggccttgg cgaccaggat
     2641 ggcgccttcg ccttccaggc gttggatggc cgtcgcctgg tacggcgcca cgaagccttt
     2701 gaggatattg ctggcggccg tcgtctccgc cccaaaggct aagaaattgt ccttggcgat
     2761 gaacggcaca ccggccagac ggccggcgtt ctcgccagcg gccaccttgg cgtcgatggc
     2821 tgcggcgcgg gcacgggcac gctcctcggt tgtggcgatg atggcgtcgt agtcggcgct
     2881 gtcggcgatg gctttgagcg accgctccac caggtcggca gccttgactt caccgttatt
     2941 gaccttggct gccaatgtcg cgatcggttc ccactcaacc atcagccgat catcctcttc
     3001 accttgagca ggtcgtcctg tacctgcggc acgtttttca agaggtcttt gggagcatag
     3061 ccatagtcga tgatggcgtc gtcacgcatc acattggtca gtccggatac ctggttggta
     3121 ggctccaggc cgtcgacatc gatagtctgc agctgttcca catactgcag aatggtggtc
     3181 aggtcgtggc tcaacgcctc aacctcatca tcgtctagag aaatgcgggc gagcgaagcc
     3241 aattttaaaa catcgtcacg cgataagttt gccatttggt acaagtatac aagaagaggc
     3301 gacagtttat aagtctttac aaagcgcagc ggcaagacta catttagccg taagcgaatc
     3361 aaaataatga cggcacttcc ccaatccgaa gcctggcctc tcccgcctca acccgcggag
     3421 atagacgaac ggcggcttca cttggcacc
//
LOCUS       DASSFD010000092         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_337726, whole genome shotgun sequence.
ACCESSION   DASSFD010000092 DASSFD010000000
VERSION     DASSFD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337726"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..491
                     /locus_tag="VFH39_02070"
     CDS             186..491
                     /locus_tag="VFH39_02070"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6924592.1"
                     /translation="MTYTSFDHFFQTLANKQRVHILQLLADKGPLSVSAIAEMLGLEQ
                     SSASHSLKQLLLCHFVAVKQIGKERIYAINEDTVKPLFDHIQQHVSKYCVQGCDHWE"
     gene            505..1218
                     /locus_tag="VFH39_02075"
     CDS             505..1218
                     /locus_tag="VFH39_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6924593.1"
                     /translation="MLTNIKLIKKEEIATGTMAFHFEKPEGFEFRAGQFLDITLLDPP
                     ETDAEGDTRGFSIASAPYEAELMVATRLRDTAFKRVLRDLPLGTELKLDAPYGDFTLH
                     KTETTPAVFLIGGIGVTPVRSMVAQAIHDGSAQQLTLIHANRTPADAPFAADFQQLVS
                     VSTNFNFVPVATDEAPDDWTGERGHVDEAMLRRHLSDLNAPIYYLAGPQGMVKAMRQL
                     LVDIGVNEDNIRTEEFTGY"
     gene            complement(1349..1690)
                     /locus_tag="VFH39_02080"
     CDS             complement(1349..1690)
                     /locus_tag="VFH39_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924594.1"
                     /translation="MVRRLLGAPLTEAELRKREAEKHMYKAELGALAVREAISSGDDQ
                     LRLYGEAMIAKAADAIATHPDQALAQTMARGMAGPLSIRLTAGNPYVHPAIVDRATAI
                     PRDSSEQPAND"
     gene            1919..2275
                     /locus_tag="VFH39_02085"
     CDS             1919..2275
                     /locus_tag="VFH39_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6924595.1"
                     /translation="MSDSSDNNLIDQSEVQMRKGMLVYCVLLLLKDGKVYTSEIIRSL
                     RQAELIVVEGTLYPLLNRLAKDKLLAYEWQESEQGPPRKYYWLTDEGKALLAELKATY
                     HRLHTSITTLEKGAKS"
     gene            2272..>2650
                     /locus_tag="VFH39_02090"
     CDS             2272..>2650
                     /locus_tag="VFH39_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6924596.1"
                     /translation="MNEVTKVHLGRQAFTVSVAAHKALRAYLDAIAKQVDDPDVTDEV
                     ELRMAELLSERGIQGDKVVLPADVEYLKAQLGEPQDFKADDDSARSSSQPASGRRLFR
                     DRENGMFAGVAAGLAAYFGVDVWL"
BASE COUNT          646 a          778 c          679 g          547 t
ORIGIN      
        1 ggtcggtcca atacagcata gggtggtcag ccaaaatatg cagaccgctg cggatcagta
       61 aaatcacaca aaacaaattg ataaaatgag taataacaat gacgagaggc atgctatcac
      121 tatccatgag cgtaattgta cactgttaat ccgggactat ctaggtcaaa tgttataatc
      181 ttcatatgac atatacatca ttcgaccact tcttccagac gcttgccaac aagcaacgcg
      241 tgcacatcct ccagctgctg gccgacaaag gtccgctcag cgtttctgct atcgccgaaa
      301 tgctcgggct cgagcaatcc tccgcttcgc acagtctcaa acagctactg ctctgccatt
      361 tcgtggccgt caagcagatc ggcaaagaac gtatctacgc catcaatgaa gatacggtca
      421 aaccgctgtt cgaccacatt caacagcacg tgagcaagta ctgcgtacag ggctgcgatc
      481 attgggaata aacaggaggc taccatgctt acaaatataa aactgataaa aaaggaagag
      541 atcgccaccg gcaccatggc gtttcatttc gaaaagccgg aaggttttga attccgtgcc
      601 ggccagttcc tggacatcac ccttctggac ccgccggaga ccgatgccga gggtgatacc
      661 cgcggtttct caatcgcctc cgcaccctac gaagccgagc tgatggtagc cactcgcctg
      721 cgtgatacgg ccttcaaacg ggtactccgc gacctgccgc tgggtaccga gcttaagctc
      781 gatgccccgt acggtgactt cacactccac aagaccgaga cgacaccggc tgtcttcctt
      841 atcggcggta tcggcgttac gccggtacgc agcatggtcg cccaagcgat acacgacggc
      901 tcggcacagc agttaacgtt gatccatgcc aaccggacac cggcagatgc accctttgca
      961 gccgacttcc agcaacttgt atccgtcagc accaacttca acttcgtgcc ggtggcgacc
     1021 gacgaagcgc cggatgactg gacaggcgaa cgtggccatg tcgacgaagc catgctgcgc
     1081 cggcatctct ccgacctgaa cgcccccatt tattacttgg ctggcccgca gggcatggtc
     1141 aaagccatgc gccagctttt ggtggacata ggggtgaacg aagacaatat ccgaaccgag
     1201 gagttcacgg gctactaaag gctctgcggg agcccgcata gtcgttagta gtaccgacaa
     1261 tccgtagcca ggaatctcct ggctacgttc ttagcgacaa catacctact gcacggtgga
     1321 cggcaaaacc attttggcca gcgtagcgtt aatcattagc cggttgctcg ctgctgtcac
     1381 gaggaattgc cgtcgcccta tcgactatcg cggggtgcac atagggattt cccgctgtca
     1441 gccgtatcga caatggaccg gccatgcccc gtgccatcgt ctgtgccagt gcttgatccg
     1501 ggtgcgtggc gatagcatca gccgctttgg ctatcatggc ctcgccgtac aagcgcaatt
     1561 ggtcatcgcc actactgatg gcttcgcgaa ccgctaacgc acccagctcc gctttataca
     1621 tgtgtttttc ggcttcacgc ttacgcaatt ctgcttcggt caacggggca cccagcaatc
     1681 gccgcaccat ggaccgcgga ccccataaca ctactgcatc aatagtgcgg acggccctgc
     1741 tttgccgcac cgcatcatac cgcttgctta tctccatata taccccctat ttttacacac
     1801 aaagcaccga gtatagccca gtctgcagcc agaggttgtt acctagatca cattttctcg
     1861 tttagataat gatgaagtat attgacataa cctactacct tgtatagcat agtagtatat
     1921 gagcgatagt agcgacaaca atctgatcga tcaatccgaa gtccagatgc gtaaaggcat
     1981 gctggtctac tgtgtgttac tgctccttaa ggacggcaaa gtctacacca gcgaaatcat
     2041 ccgttccctg cgccaggccg agctgattgt agtggaaggc acactctacc ccctgctcaa
     2101 ccgtctggcc aaagataaac tgctggccta cgaatggcaa gagagcgagc aaggaccacc
     2161 gcgcaaatac tactggctga ccgacgaagg caaagcactg ctggccgagc tgaaggccac
     2221 ctaccaccgc ttgcacacat caataaccac gctcgagaaa ggggccaagt catgaacgaa
     2281 gtaaccaagg tgcatctggg ccgccaggcc ttcaccgtat cggtcgcagc ccacaaggcg
     2341 ttgcgcgcct acctggatgc catcgccaaa caagtcgacg atccggatgt aacggatgaa
     2401 gtggaattgc gcatggcgga gctgttaagt gaacgcggca tacagggcga caaggttgtc
     2461 ttgccggccg atgtcgagta cctgaaggcg cagctcggcg agccgcaaga tttcaaggcc
     2521 gatgatgact ccgcccggtc aagcagccaa ccggcgagcg gccgacggct gttccgtgac
     2581 cgcgaaaacg gtatgttcgc cggtgtagcc gccggcctgg ccgcctactt cggggtggac
     2641 gtgtggctgg
//
LOCUS       DASSFD010000093         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1022773, whole genome shotgun sequence.
ACCESSION   DASSFD010000093 DASSFD010000000
VERSION     DASSFD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022773"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..533)
                     /locus_tag="VFH39_02095"
     CDS             complement(63..533)
                     /locus_tag="VFH39_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6924597.1"
                     /translation="MLKQLKTRKSEGFTIIEVLIVLAIAGLILLVVLLAVPALQRNAR
                     NTTIKNDASALAGGFSEYVSNNDGKAPASPLAQNGANVTIGATGTSQATAKVSGSTTV
                     NVAKATPTTFAAGQLWWNTGKTCDGTANSRAIAVYYYIEVSGTPSNTPKCVDAA"
     gene            complement(627..1859)
                     /locus_tag="VFH39_02100"
     CDS             complement(627..1859)
                     /locus_tag="VFH39_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6924598.1"
                     /translation="MLTYQYTARNTSTGELVKADVQADSEGAAVRLIRDQGLTPVNVK
                     LQEAGFGGFSFGNKVKAKDRVLFSRQLSTLINAGLPLLQSLRSVNQQTTSKPLKVVLN
                     QIITDVEGGATLATAMGKHPRVFNQVYISLIAAGETSGTLDKALERLAIQQEKDADLA
                     SKVRGALMYPVIVLLVMVGVVGFMLVKVLPQVKSIYAGLPGDNSLPLLTSVLLGVSDF
                     IIHFWWVIIIVLGVGIFFTTRWARTGPGKEVIDKLKMRMPPLGSLFMKMYMARFARTG
                     STLVASGVPLLQMLQITSEAVSNVHIKGSLERASEKVKGGKSLADALENDPNFLPLVP
                     NMLKIGEQSGAMEEMLSKAADYYEKEVDNEIAAVSSIIEPIMMVLLGVVAFLIVGAVL
                     LPIYGLAGNSSFTSGAGA"
     gene            complement(1864..2931)
                     /locus_tag="VFH39_02105"
     CDS             complement(1864..2931)
                     /locus_tag="VFH39_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET6924599.1"
                     /translation="MAAAPRIEILLEEVVKKKASDLHLQVGLPPMLRVDGSLVPVSGA
                     EVLTEETVETLIFAILDEDQKQILLKDKEFDFSFAFGELGRFRVNAFHERGNLAAALR
                     LIPNEIMSIEQLGLPQVVSKFADYPRGLVLVTGPTGSGKSTSLAAMISKINSERAAHI
                     ITIEDPIEYTHKSKKSVIVQREVHYDTYSFSAALRSALREDPDVVLIGEMRDLETIAS
                     AITIAETGHLVFATLHTNSAAQSIDRMIDVFPPHQQPQIRAQLSNILMAICSQRLVPA
                     IGGGRIAAAEILVATPAVRNIIREGKTHQLDAVIQTGAEFGMQSMDKQLVSLIHAGTI
                     SYDEARNFAVDLDELDRLMRD"
     gene            complement(3016..>3987)
                     /locus_tag="VFH39_02110"
     CDS             complement(3016..>3987)
                     /locus_tag="VFH39_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET6924600.1"
                     /translation="LRISISPVVWGEQVVIRLLDKSGTSLKLEDMGYTGRALRTIKKG
                     LERTNGMILTSGPTGSGKSTSLYALLSEVKSDAVNIVTLEDPVEYKMEGVNQIQVNAD
                     VGLTFSSGLRSILRQDPDIVMVGEIRDKETAELAVQASLTGHLVFSTLHTNSAAGILP
                     RLLDMGIEPFLIASTVHTVIGQRLVRRIGDHKETYQSDSAETAAIKEALGSLLPKDKA
                     DVAKVAEDLGYETLPLADDNAYTLTRGIDSPGTPGGYQGRMGVYEVFDISERIQELIL
                     KRATSSEIEAVAIEEGMVTMRQDGYLKALAGKTTVSEVNRVASANSA"
BASE COUNT          740 a         1155 c         1168 g          924 t
ORIGIN      
        1 attaccacaa aagaaacgac cgccctctta tggagcggtc gtttcgattt cgtgcctggt
       61 ctttaggcag cgtcgacaca cttgggtgtg ttggaaggcg taccggaaac ttctatgtag
      121 tagtagaccg cgattgcacg cgagttggcg gtgccatcac aagtcttacc ggtgttccac
      181 cacagctggc cagcagcgaa cgtagtcggc gtagctttag ccacattaac ggtcgtgcta
      241 ccgctcactt ttgcggtggc ctgcgacgta ccggtagcac cgatagtcac gttagcaccg
      301 ttctgagcaa gtggcgaagc cggagccttg ccatcattgt tcgagacgta ctccgagaag
      361 ccgccggcga gagccgaagc gtcgttcttg atggtcgtgt tacgagcgtt acgctgcaag
      421 gcaggaacgg cgagcaggac gaccaacagg ataagaccgg caatcgccag cacgatcagg
      481 acttcgatga tggtgaagcc ttcactttta cgtgttttta gttgtttaag catgtgcaaa
      541 tgcccccttt gcattatttt atacgcttat gcttgtgata atacggttgc gctttaaaaa
      601 ttgcaagctt tatcccttgt cacctcctag gcaccggcgc cgctggtaaa gctgctattg
      661 ccggcgaggc cgtaaatcgg cagcaggacg gcgccgacta tcaggaaagc gacgaccccg
      721 agcagcacca tcatgatcgg ttcgatgatc gagctgacgg cggcgatttc gttatcgact
      781 tctttctcgt agtaatcggc tgccttgctg agcatttcct ccatagcacc ggactgctcg
      841 ccgatcttca gcatgttcgg caccagtggc aggaagttcg ggtcgttttc gagcgcgtcg
      901 gccagtgact taccgccttt gaccttttca ctggcacgct cgagggaccc cttgatatgc
      961 acgttgctga ctgcctcact ggtgatctgc agcatctgca gcagcggcac accgctggcg
     1021 accaacgtgc tgccggtccg cgcaaaccgc gccatgtaca tcttcatgaa caggctgccg
     1081 agcggcggca tccgcatctt aagtttatcg atgacttcct tgcccggtcc ggtgcgggcc
     1141 cagcgggtgg tgaagaatat accaacgcct aacacgatga tgatcaccca ccagaaatgg
     1201 ataatgaagt ctgacactcc cagcagcacg ctcgtcagca atggcagcga gttgtcgccc
     1261 ggcagcccgg cataaatgct tttgacctgg ggcagtacct tgaccagcat aaagcccacc
     1321 acgcctacca tcaccaatag cacgatgacc gggtacatca gcgcgccccg cactttgcta
     1381 gccaggtcgg cgtctttctc ttgctggatg gccaggcgtt ccagggcttt gtcgagcgta
     1441 ccggaggttt caccggcggc aatcaggctt atgtacacct ggttgaagac ccgcgggtgc
     1501 ttgcccatgg cggtcgccag ggtggctccg ccctcaacgt cggtgatgat ctggttcagc
     1561 actactttga gcggcttgct ggtagtctgc tggttgacgc tgcgtaagct ttgcagcagc
     1621 ggcaggccgg cattgataag cgtgctcagc tgccggctga acaatacgcg gtctttggcc
     1681 ttcaccttgt tgccgaagct gaagccgccg aacccggctt cctgcagctt gacattgacc
     1741 ggcgtcagac cctgatcgcg gataagcctg acggctgcgc cttcgctatc ggcctgtaca
     1801 tcggccttaa ccagttcacc ggtcgaggtg ttgcgggcgg tgtactggta ggtcagcatg
     1861 gtgtcagtcc ctcattaatc ggtcgagctc gtccaggtcg accgcgaagt tacgggcttc
     1921 atcgtagctg atggtaccgg catggatgag gctcaccaac tgtttgtcca tgctttgcat
     1981 gccgaactcg gcaccggtct ggataacggc gtcgagctga tgggtcttgc cttcacggat
     2041 gatgttgcgc accgccggcg tcgcgaccag aatctcggcg gcggcgatac gaccgccacc
     2101 gatggcgggg accaggcgct ggctgcagat agccatgagg atattgctga gctgagcgcg
     2161 gatctgcggc tgctggtgcg gcgggaacac gtcgatcata cggtcgatac tttgggcggc
     2221 actgttggtg tgcagcgtgg cgaataccaa gtggcccgtt tcggcgatgg tgatggcact
     2281 ggcgatggtt tcgaggtcac gcatctcacc gatcagcacc acgtccgggt cttcgcggag
     2341 ggccgaacgg agagcggcgc tgaaactgta agtgtcgtag tggacttccc gctgcacgat
     2401 gaccgacttc ttggacttgt gggtgtattc gatcgggtct tcgatggtga tgatgtgtgc
     2461 ggcgcgctcg ctgttgatct tgctgatcat cgccgccagg ctggtcgatt tgccggagcc
     2521 ggtcggaccc gttaccagta ccaggccgcg cgggtaatcc gcgaacttgc tgacgacttg
     2581 cggcagcccg agctgctcga tactcatgat ctcgttcggg ataagccgga gggcggccgc
     2641 caggttgccg cgttcgtgga aggcattcac acggaaccgt cccaactcgc cgaacgcgaa
     2701 gctgaagtcg aattccttgt ctttgagcag gatctgtttc tggtcttcgt caaggatggc
     2761 gaagatgagc gtctcgaccg tttcttcggt cagaacttcg gcgccggata ccggcaccag
     2821 cgagccgtcc acacgcagca tcggcggcag gccgacctgc aggtgaaggt ccgaagcttt
     2881 cttcttgacg acttcctcga gcaatatctc gatgcgtgga gcggccgcca ttacgcaacc
     2941 ctcctggtgg ttttaccctt ggtttttgtg tcgtgtgttg ttttcactgg tttttgccct
     3001 tcccctttat ctgcctcagg cgctattggc tgacgccacc cggttcactt cgctgacggt
     3061 cgttttgcca gccaatgcct tcaggtaacc gtcctgtcgc atcgtcacca tgccttcttc
     3121 tatagctacc gcttcgattt cggagctggt agcacgctta aggatcaatt cctggatacg
     3181 ctcactgatg tcgaacactt cgtaaacgcc catccggcct tgataacctc caggggtacc
     3241 cgggctatca attcctctgg ttaaagtata agcattatca tcagctaatg gcaaggtttc
     3301 gtagccgagg tcctcggcca ccttagcgac gtcggctttg tccttgggca gcaacgagcc
     3361 caacgcttcc tttatggcgg ccgtttcggc gctgtccgat tggtacgttt ccttgtggtc
     3421 gccgatgcgc cgcaccagtc gctgaccgat cacggtgtgc accgtgctgg cgatcaggaa
     3481 cggttcgata cccatgtcca gaagtcgcgg cagaatgccg gcagcgctgt tggtgtgcag
     3541 cgtgctgaat accaggtgcc cggtgagtga ggcctgcacc gccagctcgg cagtctcttt
     3601 atcacgaatc tctcccacca tgacgatgtc ggggtcctgt cggaggatgg agcgcagacc
     3661 gctcgagaac gtcagaccga cgtcggcgtt gacctggatc tgattgacgc cttccatctt
     3721 gtactcgacc gggtcctcga gcgtcacgat gttgacggcg tcgctcttga cctcgctcag
     3781 cagcgcgtag aggctggtcg acttaccgga accggtcggc ccggaggtca gaatcatgcc
     3841 gttggtgcgc tccaaaccct tctttatggt gcgcagcgcc cggccggtgt agcccatgtc
     3901 ttccagcttc aggctggtgc cggatttgtc gagcagacgg atgaccacct gttcgcccca
     3961 caccaccggg ctgatggaaa tacgcag
//
LOCUS       DASSFD010000094         2803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_479025, whole genome shotgun sequence.
ACCESSION   DASSFD010000094 DASSFD010000000
VERSION     DASSFD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479025"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFH39_02115"
     CDS             <1..196
                     /locus_tag="VFH39_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924601.1"
                     /translation="IPLFYTATILLLYSANNLAAGPAIAVQRVAYNLVGIAIGMLVIL
                     YPFPMLVKQIHKIDPVLKLK"
     gene            273..473
                     /locus_tag="VFH39_02120"
     CDS             273..473
                     /locus_tag="VFH39_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924602.1"
                     /translation="MPEENQIHNNEDYGDCDIGEEPFLELVPEKQDIHRNNNGHQECD
                     ERERHQATAHRDLFTMPVASGL"
     gene            complement(760..1293)
                     /locus_tag="VFH39_02125"
     CDS             complement(760..1293)
                     /locus_tag="VFH39_02125"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6924603.1"
                     /translation="MAKAARAIIIEGDRMLVMFRNKQGSQYFTLVGGKVADGESTEQG
                     LIREVKEETGLDITSAQLVFTEEHPEPYNQQYMYLCAVAPHAEVALDEYSEEYLLNQL
                     STNIHEPLWVTTASFERLPFRTPSLQAAIVYCLRNGFPAQPVSLTDNTFYIEQMQRRQ
                     HGMASLPKRLLGKLKKS"
     gene            complement(1381..1740)
                     /locus_tag="VFH39_02130"
     CDS             complement(1381..1740)
                     /locus_tag="VFH39_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET6924604.1"
                     /translation="MAHSKSGKHATADAEAEFMRIRRQFREVAAAALLVLTLGAVFYH
                     FVEHFSWVNSFYFCTITLTTIGYGDVVPHTTAGKLFTIFYVLIGIGILAFFGNLLVRN
                     AVARREYRRTRKPSSRW"
     gene            complement(1780..2016)
                     /locus_tag="VFH39_02135"
     CDS             complement(1780..2016)
                     /locus_tag="VFH39_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924605.1"
                     /translation="MRILFALLGIVTGLLLVFGGYRLARLIIPLEGFLTGLSLNDAVL
                     VGLTAHAYNTTGYEFRSWSYHRPFPSFMQYPPPA"
     gene            complement(2036..>2803)
                     /locus_tag="VFH39_02140"
     CDS             complement(2036..>2803)
                     /locus_tag="VFH39_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924606.1"
                     /translation="LSGASGQLRTALGSVSARVPALQPASQKVVAVTTDLHARGDRLR
                     TVVRTIDFLAVVLPWLIIAAVLGALAFSWSRRRTIIAIAVVFAGLILLELIGLKLARQ
                     TVLDQVQRPENLTAVAYVYDTVVTGLKSRLYWLLGAMAAIFVLCLLAGPARWAVRLRH
                     LVRLDSLHNERFAEAWASARRWVHRYEPYLWLVALLGALVALAAAGAVTGRSVLNAGL
                     IFLSLGALVHIIATPTQLQRREQSHGMKKARLSTMNS"
BASE COUNT          650 a          791 c          770 g          592 t
ORIGIN      
        1 aattcccctc ttttatacgg cgaccatcct gctcctttat agcgccaaca atcttgctgc
       61 cggcccagcg attgccgtgc aacgcgtcgc ctacaacctg gtgggaatcg ccatcggcat
      121 gctggtgata ctgtatccgt tcccgatgct cgtgaagcaa attcataaga tcgaccctgt
      181 actgaagctg aagtaatcgg ctaaaaacgg ccccagaaag cgtattgtat ccgccagtcg
      241 ccagggctag tcgcttgcca gggcgcttac ttatgcctga agaaaatcag atacacaaca
      301 acgaagacta cggcgattgc gatattggcg aggagccgtt ccttgaactg gtgcctgaaa
      361 aacaggatat ccaccgtaac aataatggcc accaggagtg cgacgaacgc gagcgccatc
      421 aggcgactgc tcatcgtgat ctatttacca tgcctgttgc gtcgggactt taggggatga
      481 aaaatgacgt acaagttgaa gtcgtaaccc caaccgtacc atttacgggt gatgaatggt
      541 gccgcaggat tccaggtgcg ggatttgaac cgggctttcg tgggccttcg ccaatcgtag
      601 ggaacgcccg caaacgttcc tgatttttta cttttttgtg ccatacccac attatagtgc
      661 agccacagtt atttgtggaa aaaacgggct ccattgactg ctgccacctc tacgcccaaa
      721 acgcccagta acatcgctgt atagctggtg cgggcgagcc tagctctttt ttagtttacc
      781 cagcagccgt ttgggtaatg atgccatgcc atgctgccgg cgctgcattt gctctatata
      841 aaaagtattg tccgtaaggc ttacaggctg ggccggaaag ccgttgcgca ggcaataaac
      901 gatcgctgct tgcagactcg gcgtccggaa cggtaagcgc tcgaatgatg ctgtggtgac
      961 ccacagcggc tcatggatat tggtgctcaa ctggttcagc aggtattctt cggaatactc
     1021 atccagcgcg acttccgcgt ggggagccac ggcgcagaga tacatgtact gttggttgta
     1081 cggctccgga tgctcttcag taaataccaa ttgtgcactg gtaatgtcca gaccggtctc
     1141 ttccttgacc tcgcgtatca ggccttgctc ggtcgactcg ccgtcggcca ctttgccgcc
     1201 gacaagcgta aagtactgac tgccttgctt gttgcggaac atgaccagca tcctatcgcc
     1261 ttctatgatg attgcgcgtg ccgcttttgc catagcagat agtatagagc aaaagcttag
     1321 ggaagtagta gacggcatgt ggccatcgcc tactacttta ctagttataa aaaggtgtag
     1381 ctaccagcgg ctggatggtt tgcgggtgcg gcggtactcg cgccgggcta cggcattgcg
     1441 caccagcagg ttgccgaaga aggctaatat gccgatgcca atgagcacgt aaaagatggt
     1501 aaagagctta ccggcggtgg tatgcgggac aacgtccccg taaccgatcg ttgtcagggt
     1561 gatggtacag aaataaaacg agttgaccca cgaaaaatgc tcgacgaaat gataaaagac
     1621 agcgccgagc gtcagcacaa ggagagccgc cgcggcgact tcgcgaaact gccgccggat
     1681 ccgcatgaac tcggcttcgg catcggccgt agcatgcttc cctgatttcg agtgagccat
     1741 gagctcagta tacttcattt atgtcaagta tcggcttctt tacgccggcg gcggatactg
     1801 catgaaggac ggaaaaggac ggtggtatga ccacgaccgg aattcgtagc cagttgtgtt
     1861 atacgcatgg gcggttaggc caacgagaac cgcgtcgttc agcgacaaac cggtcaggaa
     1921 tccctccagc gggatgataa ggcgggccag gcggtatccg ccaaatacga gcagtaagcc
     1981 tgtcacgatg cctaacaggg cgaacagtat tctcatatcc ctcctggcca tggccttacg
     2041 aattcatagt agataaacgc gccttcttca tgccgtgact ctgctcacgc ctctgtaact
     2101 gcgtcggcgt agcgatgatg tggaccagcg ctcccaggct caggaatatc agtcccgcgt
     2161 tcagcacgct acgtccggtc actgccccgg cggcggctag cgctaccaat gcacccagca
     2221 aggcgaccaa ccataagtac ggttcgtagc gatgcaccca gcggcgggcg ctcgcccaag
     2281 cttcggcgaa gcgttcgtta tgcaggctat cgagccgtac caggtggcgt aggcgcaccg
     2341 cccagcgcgc cggaccggcc agcaggcaca ggacaaagat cgccgccatc gcccccagca
     2401 accagtacag cctgctcttc aggcctgtta ctaccgtgtc gtacacatat gctaccgctg
     2461 tcaggttctc cggacgctgc acttggtcca agaccgtctg cctagccagc ttcaagccta
     2521 tcaactccaa aagtatcagg ccggcaaaga cgacggcgat ggcaatgatg gtacggcgac
     2581 ggctccagct gaatgccaag gcacccagaa ccgcggcgat tatcaaccat ggcaacacca
     2641 ctgccaagaa atcgattgtc cgaacaacgg tgcgcaggcg gtcgccacgg gcatgcaaat
     2701 cggtggtgac cgcgacgacc ttctggctgg ccggttgcaa cgccggtacg cgagcactca
     2761 cgctgccgag ggcggtccgt agctgcccgg atgctccgct caa
//
LOCUS       DASSFD010000095         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_938400, whole genome shotgun sequence.
ACCESSION   DASSFD010000095 DASSFD010000000
VERSION     DASSFD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938400"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VFH39_02145"
     CDS             complement(<1..1153)
                     /locus_tag="VFH39_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6924607.1"
                     /translation="MPNRKDLTKQTIRFYFRHAWRYPKHVIGLAISIPCTVLANNIIP
                     PIILANVLNRLSRHDFQSHRVWASFGPELSMYAAIVILAGVTGWRIVDALDWRLEGNV
                     ERDIAREVFDHLQQQSATFHANHFGGSMVSQTNKLMGSYIRFADTTVFNVLPLLFSLL
                     FICIILGHIAVWYALIILAFAVLYIVSSFYVTRPVRRLGAEQAARESAQTGVLADAIT
                     NVMAIKSFSGSAYENKRFAKATDHTRAGLLSMLRYNQRQQAYFSTIVNVISASALLMA
                     VVGVVVFGANIATVFLLLTYTASVANQLFQFSNSALRTYNRALGDASDMIGILGITPE
                     IADPKDPETSRISRGLIDFDHVTFQHDGADDALFDDLSVRIKPGEKVGLV"
     gene            1414..>2298
                     /locus_tag="VFH39_02150"
     CDS             1414..>2298
                     /locus_tag="VFH39_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924608.1"
                     /translation="MPEFDPTSSRETSVDDPEHKSRRRRRKSREAATRTGRAATESLI
                     WQREEPKPKKAAKDIAEALKGRTRKEEVEEKEADVPASVATEADTPSKVTEQKAEAVE
                     DEADDAEAEAKLAELTPDEVHHAAEEYVQVRGMEVTAEQVGTEPDSAESAALEADAAL
                     LAAMRHKLEADPQADPEQALDEAYAETVNTLPPEEAPEEAELPMPDAETETASAAETS
                     ESTPDHNGPEASAEQPPVVPPQPPEHAPHGTPEEPEPSRTVVFPPIPPAGAPPTGEYV
                     PTPPEERFGPLRNVISVEE"
BASE COUNT          530 a          646 c          693 g          429 t
ORIGIN      
        1 ccaccaggcc gaccttttcg cccggtttga tgcggacgct caagtcgtcg aacaacgcgt
       61 cgtcggcgcc atcgtgctgg aacgtgacgt ggtcgaaatc aatcaggccg cggctgatgc
      121 gggaggtttc ggggtcttta gggtccgcaa tctcgggtgt aatacccaga atgccgatca
      181 tgtcactggc gtcgccgagc gcccggttgt acgtccgcaa tgcggagttg ctgaactgga
      241 agagctggtt ggcgacgctc gcggtgtacg tgaggagcag gaacacagtg gcgatgttgg
      301 cgccaaaaac caccacgccg actacggcca tgagtaaagc cgaggcggat atgacattga
      361 cgatggtgct gaagtacgcc tgctgccgct gattgtagcg caacatgctg agcaacccag
      421 cccgcgtgtg gtcggtggct ttggcgaagc gcttattttc gtatgccgaa ccagaaaagc
      481 ttttaatcgc catgacattc gttatggcat cggccagtac gcctgtctga gcactctcgc
      541 gggcagcctg ctcggcaccc aggcgccgta caggacgggt gacgtaaaat gagctcacga
      601 tgtaaagaac ggcaaaggcc aaaattatga gcgcatacca gaccgcgata tgcccgagta
      661 tgatgcagat gaacaagagg ctgaacagga gtggcagcac gttaaatacg gtagtgtctg
      721 caaaacggat gtaggagccc atcaatttgt ttgtttggga aaccatcgag ccgccgaaat
      781 gattagcatg gaaggtagcg ctttgttgtt gcaggtggtc aaaaacttcg cgcgcaatat
      841 cgcgctccac attgccctcc agccgccagt ccagcgcgtc gacaatccgc cagccagtga
      901 cgcccgccaa tatgacaatg gcggcataca tgcttaactc cggcccgaag ctggcccaga
      961 cgcggtgcga ctggaagtcg tgtcggctga gtcggttcaa tacgttggcc aggatgatag
     1021 gcggtatgat gttattcgct aacacagtac aggggatgct gatggctagc cctatgacgt
     1081 gcttcgggta tctccaggca tggcggaagt aaaagcgaat cgtttgtttg gtaagatctt
     1141 tgcggtttgg cataggcaca gccctccttg gtgctgtcct gaattttaga ttgttggtgg
     1201 ttaggttttc gactggctca cgctgccagt ttggtggggg atcgagactt agaagccggt
     1261 cgacggccgg gtcgcgcagt ggtcgatgcg aatagtcaga atcatgaggc cgcctccgtt
     1321 ttttgctgat atggatgtgc ccaaatcagt tgcgaataga taaaaaatat actatactat
     1381 tacttcgtaa attgcaagtg taaacattag cgtatgccag agttcgatcc cacatcctcc
     1441 cgcgagacat cggttgacga tccggagcac aagtcccggc gccgccggcg taagtcacgc
     1501 gaagcggcta cccggaccgg tcgcgccgct accgaaagcc tgatctggca gcgcgaagag
     1561 ccgaagccca agaaggctgc caaggatatc gccgaggcgc tcaaagggcg cacgcgcaaa
     1621 gaggaggttg aggagaaaga agcggacgtg ccggcatctg tcgcaactga ggccgataca
     1681 ccgtccaaag tgaccgaaca gaaggcagaa gcagtcgaag atgaagccga cgatgccgag
     1741 gccgaggcca agcttgccga actgacaccc gacgaagtcc accatgctgc cgaggagtat
     1801 gtccaggttc gcggcatgga agtcaccgcc gaacaagtcg gcaccgaacc ggactccgct
     1861 gagtctgcgg cgcttgaagc cgacgctgcc ctgctggctg ccatgcgcca caagctggag
     1921 gccgacccgc aggccgatcc ggagcaggca ctcgacgaag cgtatgccga aacggtcaat
     1981 acgttgcccc cggaagaagc ccctgaggaa gctgaattac cgatgcccga cgccgaaacg
     2041 gaaactgcat ccgcggccga gacgtctgaa tcgacacccg atcataatgg cccggaagca
     2101 tccgccgagc agccaccggt cgtgccgccg caacccccgg aacatgcccc tcatggtacg
     2161 cccgaggaac ccgagccgtc ccgcaccgtc gtgtttccgc cgatcccgcc cgccggcgcc
     2221 ccgccgaccg gcgagtatgt tcccactccg ccggaagagc gtttcggacc gttgcgcaac
     2281 gtcatatcgg tagaagaa
//
LOCUS       DASSFD010000096         8037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_932547, whole genome shotgun sequence.
ACCESSION   DASSFD010000096 DASSFD010000000
VERSION     DASSFD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932547"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="murE"
                     /locus_tag="VFH39_02155"
     CDS             <1..1059
                     /gene="murE"
                     /locus_tag="VFH39_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HET6924609.1"
                     /translation="DNDIESQVQHYTNVPVPELMRRLKKLKAANIDYLVLEITSQALA
                     QNRAWGVPFSVAVMTNVTHEHLDYHKTFERYRDAKRKLFKLANKNKKGMQVGVVNAED
                     PSAELFASDVAHPILYGVEKGDLRATEVKLTPEGSTYVARAGEDEYRITCNIPGSFNV
                     YNSLAVVAVGGALGLNRQEIEQGIAALTGVEGRMTNIDEGQDFSVIVDFAHTPDSFEK
                     LFKDLRPVVKGKLIALFGSAGRRDEAKRAVQGELAGRYCDEVIVTEEDDRDIDGQEIM
                     DQIAEGAEKAGKVRDRDLFLVHDRTEAINFAMQRAHRGDTVILLGKGHEKTIERADGA
                     HPWDEIGTAHAALRSSRS"
     gene            1099..1401
                     /locus_tag="VFH39_02160"
     CDS             1099..1401
                     /locus_tag="VFH39_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924610.1"
                     /translation="MITENNNMPAVYFTAAELIEHANAMSREIDKEKSALLALYQGGL
                     VTANTDVVVTDGGEEGITVAQLYGRLEGRETELNAFVAYLISKADEKLGKDNPDLA"
     gene            1473..1691
                     /locus_tag="VFH39_02165"
     CDS             1473..1691
                     /locus_tag="VFH39_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924611.1"
                     /translation="MNNETPSVEHIEVHEAQQDAQRITVQQAEFLAKKPVELMQLPDG
                     SLATREEVNAAQQAGQEVSGAYNNGRRA"
     gene            complement(1737..2606)
                     /locus_tag="VFH39_02170"
     CDS             complement(1737..2606)
                     /locus_tag="VFH39_02170"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HET6924612.1"
                     /translation="MSAQINKTVLMSGVDYFDDAAAINPFMDSTAPIDLVKAKAEHDA
                     IRQALESAGVTVIKVDPPAGCQDGVYTANWALVRGDQAVMSSLPNARKGEEAYAEQVL
                     QAQGKTTYRVPDQLRFSGQGDALPVGNYLLAGSTYRTDPEVHQFLADVLGYDVISLQT
                     VPQQDEAGQPVTNAYSGWPDSFFYDIDLAISVLTPNLIAWCPTAFTPESQEKILAIPD
                     LERIEVSFEEAKTAFACNLVSTGETVVMSADAPHFKAAIESKGLKVITPEIKELVKGG
                     GYIRCTTLTLDNE"
     gene            complement(2804..3127)
                     /locus_tag="VFH39_02175"
     CDS             complement(2804..3127)
                     /locus_tag="VFH39_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924613.1"
                     /translation="MQDAPAPNGLRVVQAPTGERVIQVSLKAVGLPAESDARLVATVN
                     NYTRVADTVLTSTRRLTIYPNYDGLTAKRAERELQTLFESITALIQHRLDQATKKTEL
                     NVGIG"
     gene            3227..3862
                     /locus_tag="VFH39_02180"
     CDS             3227..3862
                     /locus_tag="VFH39_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924614.1"
                     /translation="MSQFEDFKKHFNAEASQRLSREAYQLRRDLLKASGTILKQVLLL
                     DLDIRNSAEEGDRIEHWAMRDEQAAPAIDEDQIETTISFLLEGHPEPHGTPKRLRLHV
                     PVPYDFDIDDQETIESLHDPAAVYAERLDVRGSQLVTLGRYAVTPEVAYSYQAPVDRS
                     PGAADQVGTEDIDTMWAHLERRVETTIPVLKLYEDIVNWNLISQRQSTFGG"
     gene            3971..4237
                     /locus_tag="VFH39_02185"
     CDS             3971..4237
                     /locus_tag="VFH39_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6924615.1"
                     /translation="MSTPLQPLGDYVVAQAEEAETKTASGLYLPGNAQEKPKVAKIVA
                     VGPAVQQVKTGDRIVYKSYSTTEVKVGRDEYILVKEEDILATVK"
     gene            4248..5915
                     /gene="groL"
                     /locus_tag="VFH39_02190"
     CDS             4248..5915
                     /gene="groL"
                     /locus_tag="VFH39_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6924616.1"
                     /translation="MAKKVFYDDDARRRVLAGAEILYHAVKTTMGPKGRNVVISKSYG
                     APTVTHDGVSVAKGVEIADVDDETLGYKVGAELIKQAANKMNDVAGDGTTTVTVLTYH
                     ILNEANKLIAAGHNPMLLRKGLEVAAHEVVAQLSGMAEDIEGKKNRVAEVATISAGDA
                     EIGNEIAGVIEQVGKDGVVTVEQGQGLSLESEVVEGFTFDRGYVSPYMVTDTTRMEAV
                     YDKPAIVVTDKKISSIQEFLPLLEKLAQSGKKDLVLIAEDVEGEALGTLILNRLKGVF
                     NTVAIKAPAFGDRRKDILNDIAILTGAEVISEDRGMTFEEADLSVVGTARKVIVTKDD
                     TTIIEGAGSPAEVTARIKQIGAQADAATSEYDKENLEKRQAALSGKVAVIKVGGATET
                     EINEKKDRVDDAVHAVKAALAEGIVPGGGVTLINLADTIKQDEKLVVGDDGVALLNGK
                     FLLKRALEQPFRILLANSGLNPDEWLPQVRNAKPGFGVNVNDPSKLVDLKANGVVDPA
                     RVTKEAVQNAVSIAGTSMTMGALVVEVPEPKSAAPADGGMGAMGGMM"
     gene            complement(6057..7085)
                     /locus_tag="VFH39_02195"
     CDS             complement(6057..7085)
                     /locus_tag="VFH39_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924617.1"
                     /translation="MRASTLSSINKANRSFMTLIRQLPSGGISMLAAVFLIGAVGSYY
                     LFTTHAATPTASVQTESGTVTSPALTVGDTSASGSHAVKFAKAATSGTALHYAGNSNF
                     GANGQYLPGAYGFNLADVGSVSDLTSLPAGVKGLAWVGQCNGADATFTNTVKPYIGHP
                     KLFGFYLMDEPDATGQWAPKCPIANLKAESDWIHANVPGAKTFIISLNMASSSNPSYA
                     GTYNSTNSDIDLFGLDPYPCRTELSGCDYTYITKAVPAAVAAGINKSAIVPVYQTFGL
                     GKWVDDGGSTYAVPTATQAQKIIDTWASVVPNPVFDYAYSWGSQNSDTALESMPAVQQ
                     VFLAHNTQ"
     gene            7189..7743
                     /locus_tag="VFH39_02200"
     CDS             7189..7743
                     /locus_tag="VFH39_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924618.1"
                     /translation="MALGNSQKDSDKVPPELAPYYSSKRRPSWLRWVIGGIILVVVIL
                     LLIWLFVWAWHHSHQNTAQTKQPTTSQSQQESNGAGNSGSSGSGAQNNGNRSDSSTGN
                     SSPNAALPGDDSGGAATSGGANATNPSSSNNNSGTGSGSGTRTATPTTTPNTGPAGEA
                     AVFVASTLFGTVLYQLRLRRKTDG"
     gene            7750..>8037
                     /locus_tag="VFH39_02205"
     CDS             7750..>8037
                     /locus_tag="VFH39_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924619.1"
                     /translation="MFESDFVPSVQEPTPEERAYALNLLNESLKRNLRSTVEELPPDA
                     QDILWRDDVVMHERAVFERDDTSIIPERILKPGEPLQLGDILNMLSVYFWWN"
BASE COUNT         1840 a         2020 c         2595 g         1582 t
ORIGIN      
        1 gacaacgaca tcgagagcca ggtgcagcac tacaccaacg tgccggtgcc ggagctgatg
       61 cgacggctca aaaagctcaa ggcggccaac atcgattact tggtgctgga gattaccagc
      121 caggcgctgg cccagaaccg ggcgtggggc gtgccgttca gcgtggcggt catgaccaat
      181 gtgacgcatg aacacctgga ttatcacaag acgttcgagc gctaccgcga cgccaagcgc
      241 aagctgttca agctggccaa caagaacaaa aaaggcatgc aggtgggcgt ggtgaatgcc
      301 gaagacccga gtgccgaact gttcgcgagc gacgtggcgc acccgatttt gtacggcgta
      361 gagaaggggg atttgcgggc aacggaagtg aagctgacgc cggagggcag cacatatgtg
      421 gcgcgggccg gcgaggacga gtaccgcata acctgcaaca tcccgggcag ctttaatgtg
      481 tacaactcgc tggccgtggt ggccgtgggt ggcgcgctgg ggctgaaccg gcaggaaatt
      541 gagcagggca ttgctgcttt aacaggggtt gaggggcgga tgacgaacat cgacgagggc
      601 caggacttca gcgtcatcgt ggacttcgcg catacgccgg acagcttcga gaagttgttc
      661 aaagacctta ggccggtggt aaaaggtaag ctgatagccc tgttcggctc ggctggccgg
      721 cgtgacgagg ccaaacgggc ggtgcagggc gaactggccg gccggtactg cgacgaggtg
      781 atcgtgacag aagaggacga ccgcgacatc gacgggcagg agattatgga tcagatcgcc
      841 gaaggagccg agaaggccgg caaggtgcgc gaccgcgacc tgtttttggt acacgaccgc
      901 acggaagcca taaacttcgc catgcaacgg gctcacagag gcgacacggt gattctgcta
      961 ggcaaagggc acgaaaagac catagagcgg gccgacggcg cccacccctg ggacgaaatc
     1021 ggtacagccc acgctgcgct gcgaagcagt cgcagctaat ctttgtaata ttagacaact
     1081 tgtgttatta tatttttaat gattacagag aacaataata tgccggcggt gtattttacg
     1141 gccgctgaac ttatcgagca tgctaatgcg atgagccgag aaatagataa agaaaaaagt
     1201 gctctgcttg ctttatatca aggcggtttg gtaactgcca acaccgatgt ggtggtcact
     1261 gatggaggtg aagagggtat aaccgttgcg caactttatg gcaggctgga agggcgagaa
     1321 acagagctga atgcctttgt ggcgtacttg atttccaagg cggacgaaaa acttggtaag
     1381 gacaaccctg acttagcata ataagtttga caaaaatata aataagtgct aacatgttta
     1441 tgttaagtca aaataaaaag taaggaaaaa ctgtgaacaa cgaaacaccg agtgtcgaac
     1501 acattgaagt ccatgaagct cagcaggatg cgcaacggat taccgtgcaa caggctgaat
     1561 tcctggctaa gaagccagta gaacttatgc aactccctga cggctccctc gctacccgcg
     1621 aagaagtgaa tgcggcccag caggccggcc aggaagttag cggtgcctac aataacggtc
     1681 ggcgcgccta aaatttaagc acctgctaaa tagggctccc cagcggagcc ctatttttat
     1741 tcgttgtcga gggttagggt ggtgcaacgg atgtagccgc cgcctttcac aagctctttg
     1801 atttcggggg tgataacttt gagtcctttg gactcgatag cagccttgaa gtgcggtgcg
     1861 tcggcgctca tgaccacggt ttcgccggtg gagacgaggt tgcaagcgaa cgcggtcttg
     1921 gcttcttcga atgagacttc gatacgctcg agatcgggaa tagccagaat cttttcttgg
     1981 ctttcggggg tgaaagcggt cgggcaccag gcgatgaggt tgggtgtgag cacggagatg
     2041 gcaaggtcta tgtcgtagaa gaagctgtcc ggccagccgg agtaggcgtt ggtgaccggt
     2101 tgaccggcct cgtcctgctg cgggacggtc tgcaggctga tgacgtcgta gccgaggacg
     2161 tcggccagga actggtgaac ctccgggtcg gtgcggtagg tgctgccggc tagcagatag
     2221 ttgccgaccg gcagggcgtc accctgaccg ctgaaacgga gctggtcggg cacgcggtag
     2281 gtggtcttgc cttgggcctg caggacttgc tcggcgtagg cctcttcgcc cttgcgggca
     2341 ttgggcagcg agctcatgac agcctgatcg ccgcgtacca gcgcccagtt ggcggtatag
     2401 acgccgtcct gacagccggc cggcgggtcg accttgatga cggtaacacc ggcggattcc
     2461 agcgcctgac ggatggcgtc gtgctcggcc ttggccttga ccaggtcgat gggcgccgtg
     2521 ctgtccatga agggatttat agcggcagca tcgtcgaaat aatcgacgcc ggacatgagg
     2581 accgtcttgt tgatctgcgc agacatgagg ataagtatag caaagcggag gcaacgggaa
     2641 ggtgagccgg gttcgatgag tgttaattgt gtttgttgat tgcctttagg gcaagagacg
     2701 taagcctcaa acacattcat cgaacccgga acccggagag cgcgcggcgt ggcgcacatg
     2761 ggtggtggcg tcagccacgc gctctccagg agggtccgtg ttgtcagccg atgccgacgt
     2821 tcagctccgt cttcttggtc gcctggtcga ggcggtgttg gatgagagcg gtgatgctct
     2881 cgaagagggt ctgcagttcg cgctccgcgc gcttggccgt gaggccgtcg tagttggggt
     2941 agatggtcag ccgacgagtg ctcgtcagca cggtgtccgc gacgcgggtg tagttgttca
     3001 ccgttgccac gagcctggca tcgctctcgg cgggtaagcc gactgctttg agcgacacct
     3061 ggatgacacg ttccccggtc ggtgcctgga cgacacgcag gccgtttgga gctggtgcat
     3121 cttgcattgg agcctccatc acattggagc caacaggaca gtgaaaattc taacacaggt
     3181 atattctatt gacaacagct aataattttg ttgttatata tacctaatga gtcagtttga
     3241 ggatttcaag aagcacttta acgctgaagc gagccaacgc ctctcccgag aggcatacca
     3301 gcttcgccgt gacctactga aggcttccgg gacgattctg aaacaagtat tgctgctcga
     3361 tttagatatt cgcaattcgg cagaggaggg cgataggatt gagcattggg cgatgcgtga
     3421 cgagcaggcg gcaccagcaa ttgacgagga ccagatagaa acgaccatat ctttcctttt
     3481 agaggggcat cctgaaccgc acggtacgcc caagcgcctg cgcttgcatg tgcccgtccc
     3541 atacgatttc gatattgacg atcaagagac gatcgaatcc ctacatgatc ctgctgccgt
     3601 ctacgccgag cgtctggatg tgcggggtag ccagctcgta accttgggcc gctacgccgt
     3661 taccccagaa gtggcttaca gctatcaagc gccagttgat agatccccgg gtgcggctga
     3721 ccaggtggga acagaagata tcgatacgat gtgggctcat ttggaacgtc gagtagaaac
     3781 aacgataccc gttctaaagc tgtacgaaga tatagtgaac tggaatctca tatcgcagcg
     3841 gcaaagtacc ttcggcggct aagactgcgt gcgcttgacg ctgtaagaat gctcagctac
     3901 tattaaagct ggtattagca ctcgtttggt ttgagtgcta acaaaatcag ctaacaggag
     3961 gaacgtattg atgagtacgc cgttgcagcc actcggggat tacgtagtgg cccaggccga
     4021 ggaggccgag actaagaccg ccagcgggct atacctgcct ggtaacgcgc aggagaagcc
     4081 gaaggtggcg aagatcgtcg ccgtcgggcc ggccgtgcag caggtgaaga ccggcgaccg
     4141 gattgtgtac aaaagttaca gcacgaccga agtaaaagtc ggccgcgacg agtacatcct
     4201 ggtcaaagaa gaagacattt tagcgacggt gaaataggag gagcacgatg gcgaagaaag
     4261 tattttatga cgatgacgcc cgacggcgcg tgctggccgg ggctgagatt ttataccacg
     4321 cggtgaagac caccatgggc ccgaagggcc gtaacgtggt gattagcaag agctatggcg
     4381 cgccgacggt gacacatgac ggtgtcagtg tggcgaaggg cgtggagatc gccgatgtcg
     4441 acgacgaaac gctgggctac aaggtcggtg ccgagctgat caagcaggct gcgaacaaaa
     4501 tgaatgacgt ggccggtgac ggtactacta ccgtgacagt gttgacgtat cacattttga
     4561 acgaggcgaa caaacttatt gccgccgggc acaacccgat gttgctgcgt aagggcttgg
     4621 aggttgcggc tcacgaagtc gtcgcccagt tgagcggtat ggccgaagat attgaaggca
     4681 agaaaaaccg cgtggccgag gtggcgacca tttccgccgg tgacgccgag atcggtaatg
     4741 agatcgccgg cgtgattgag caggtcggga aagacggtgt ggtcacggtg gagcagggcc
     4801 agggcttgtc gctcgagagc gaagtggtgg aaggcttcac cttcgaccgc ggctacgtga
     4861 gcccgtacat ggtgaccgac acgacccgca tggaagcggt gtacgacaag ccggcgattg
     4921 tagtgaccga caagaagatt tcctccattc aggagttcct gccgctgctc gaaaagctgg
     4981 cgcagagcgg taagaaggac ctggtgctga tcgccgaaga cgttgagggc gaagccctgg
     5041 ggacgcttat tttgaaccgg ctgaagggtg tgttcaacac cgtggccata aaggcgccgg
     5101 ccttcggtga ccgccgcaag gatattttga acgacatcgc cattttgacg ggtgccgaag
     5161 ttatcagcga agaccgcggc atgacgttcg aggaggctga cctgagcgta gtcggtacgg
     5221 cgcgcaaggt catcgtgacc aaggacgaca cgaccatcat cgaaggtgcc ggttcgccgg
     5281 ccgaggtaac ggcacggatc aagcagatcg gtgcccaggc cgacgccgcc actagcgagt
     5341 acgacaaaga aaacctcgag aagcggcagg ccgcgctcag cggcaaagtg gcggtgatca
     5401 aagtcggtgg tgccaccgag accgagatca atgaaaagaa agaccgcgtc gatgatgccg
     5461 tgcacgccgt gaaggctgct ttggccgaag gtatagtgcc gggcggtggc gtgacgctca
     5521 tcaatttggc ggacacgatc aagcaggacg agaaactggt cgtcggcgat gacggtgtcg
     5581 cattgctcaa cggtaaattc ctgctgaaac gagcgctgga gcaaccgttc cgcatcttgc
     5641 ttgccaactc tggcctgaat ccggacgagt ggctgccaca agtacgtaat gcgaagccgg
     5701 gctttggtgt aaatgtgaat gacccgagca agctggtaga cctgaaggcc aacggggtcg
     5761 tcgacccagc ccgggtgacc aaagaagcgg tccagaacgc tgtttccatc gccggaacta
     5821 gcatgaccat gggtgcgctg gtagtcgagg tgccggaacc gaagagcgct gcaccggcgg
     5881 acggtggtat gggcgccatg ggtggcatga tgtaaggtaa tgagggaacc gggataggtt
     5941 ccctcatcgc gccggcaaaa taggcatttt gtcgggctgc ttcttcctga aagaagccaa
     6001 ataaaccgcg gaatgaatcc gcggtttatt tgtagttaat tgattcaaat cttgagttac
     6061 tgggtgttgt gggcgaggaa gacttgctgg acggccggca tggattcgag ggcagtgtcg
     6121 ctgttttgcg agccccagct gtaggcgtaa tcgaagacag ggttcggcac gaccgaggcc
     6181 caggtgtcga tgatcttctg ggcctgggtg gcggtcggta cggcgtaggt gctgccgccg
     6241 tcatccaccc atttgccgag accgaaggtt tggtataccg gcacgatggc cgatttgttg
     6301 atgccggccg cgacggccgc cgggacggcc ttggtgatgt aggtgtagtc gcagccgctg
     6361 agttcggtgc ggcaagggta ggggtcgaga ccgaacagat cgatgtctga gttggtggag
     6421 ttataggtgc cggcatatga agggttgctc gacgatgcca tgttcagcga aatgatgaat
     6481 gtcttggcgc cgggtacgtt ggcgtggatc cagtcggact cggccttgag gttggcaatg
     6541 gggcatttgg gcgcccactg gccggtcgcg tcgggctcgt ccataagata gaagccaaag
     6601 agtttcgggt gaccgatgta cggtttgaca gtgttagtga aggtcgcatc ggcgccgttg
     6661 cactgaccga cccaagcgag gcctttgacg ccggccggca aggaggtgag gtcggaaacg
     6721 ctgccgacat ccgccagatt gaagccgtac gcccccggca gatactggcc gttggctccg
     6781 aagttactat tgccggcgta gtgcagggca gtgccgcttg tggcggcttt ggcgaacttt
     6841 accgcatggc tacccgatgc cgatgtatcg ccgaccgtca gggctgggct ggtgacggtg
     6901 ccgctttcgg tctgtaccga cgcggtcggt gtggcggcat gtgtagtgaa cagatagtat
     6961 gaaccgacag ccccgataag gaagacggcc gccagcatgc taatgccgcc cgacggcagc
     7021 tgccggatga gcgtcatgaa ggaacgattg gccttattga tagatgacag ggtgctggcc
     7081 ctcatggccg gaagtatacg ccaagccgct tatggcagca atgacacggc aacgaccgct
     7141 gtgacgctcg tgctttttat actggacaga ggggggtaaa atgacactat ggctttggga
     7201 aatagtcaaa aggatagtga taaagtaccc ccggaactgg cgccgtacta cagttcgaag
     7261 cgtcgcccgt cctggctgcg ctgggtgatc ggcggcatca ttctcgtcgt tgtcatctta
     7321 ttactgatat ggttgttcgt ttgggcctgg caccattcgc accagaacac ggcccagacc
     7381 aagcagccga cgaccagtca gtcacagcag gagtcaaacg gagcaggtaa ttccggctcg
     7441 tcgggttcag gcgcccagaa caacggcaac cgttcagata gcagcaccgg caattcctca
     7501 ccgaatgcgg cgctgccagg ggatgacagc ggcggggctg cgacgtccgg cggcgctaac
     7561 gcaacgaatc catcgagctc aaataacaac agcgggacag ggagcggttc cggtacgcgt
     7621 accgcaacgc cgaccaccac gccgaacacc ggtccggctg gcgaggcggc ggtatttgtg
     7681 gcttccacac tgtttggcac ggtgctctat cagctgcggc tgcgccgcaa aacagacggt
     7741 tgataccgta tgttcgaaag cgatttcgtg ccctctgtgc aagagcctac ccccgaggag
     7801 cgggcctatg cgctcaacct actgaacgaa agcctcaaaa gaaatttgag gagcactgta
     7861 gaagagttgc ccccggacgc ccaggacata ttgtggaggg acgacgtcgt aatgcatgag
     7921 cgcgcggttt ttgagcgtga cgacaccagt attattcccg agcgcatact gaagccaggt
     7981 gagcccctcc agctggggga catactgaat atgctcagcg tgtacttctg gtggaac
//
LOCUS       DASSFD010000097         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_119438, whole genome shotgun sequence.
ACCESSION   DASSFD010000097 DASSFD010000000
VERSION     DASSFD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119438"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFH39_02210"
     CDS             complement(<1..852)
                     /locus_tag="VFH39_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET6924620.1"
                     /translation="MAKSEEFLRRSAEAPAALLDRTIVAGQQVKAALPAGKVIKKTED
                     YWHTLGPGLTTGAADDDPSGIATYSQTGARYGYQLLWLAAWVYPLAAAIQEMCARIGM
                     VTGRGLAANIRVHFSRRTLHICTLLLFVANAFNIGADLGAMAKGVQLLNPHLRFWWLV
                     VAFALLSLLLQIFTPYVRYARYLKWLAMVLLAYVFSALLAHLNWSNVAAHAVIPHMHW
                     GKEQLLIVCGILGTTISPYLFFWQTSQEVEERILQGKTTLQARQAATTPADIKDMRID
                     VWSGMLLS"
     gene            complement(924..2090)
                     /locus_tag="VFH39_02215"
     CDS             complement(924..2090)
                     /locus_tag="VFH39_02215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HET6924621.1"
                     /translation="MTVDILIVLFLISALVRGREIGFVQQLFSTVGFFGGLLLGTFIE
                     PHTVSLVHSPLSRSLVTLLTTLGTAFLLLIIGEYIGIVLKHKVQEKAVNKADNLFGSL
                     LSAASVLLAVWLGAAILSSVPYPSLKNAIRASRIITALDSNLPPAPNIIADLGHLIDP
                     NGFPNVFIGSEPTPPDNINLPPSSALAAAVAKDKASIVKIEGQGCGGIVEGSGFVVGN
                     NLVATNAHVVAGIAHPYVKDANGTHTAVAIWFDPDLDFAVLRVANLAGDPLLINSSRV
                     PQSTPAAVLGYPGGGGFTAEPASVLDEFTATGRNIYDRGSTERDVYAVAANIIPGNSG
                     GPLVTADGAVVGVVFAQSTAYNHVGYALTTAAVAQEINQAAAQDHAVSTGGCAE"
BASE COUNT          444 a          637 c          720 g          442 t
ORIGIN      
        1 gctcagcagc atgcccgacc agacatcgat gcgcatatcc ttgatatcgg ctggcgtggt
       61 ggcggcctgc cgtgcctgca gcgtggtctt gccctgcaag atgcgctctt ccacctcttg
      121 ggaggtctgc cagaagaaaa ggtacggcga tatggtggtg ccgaggattc cgcacacgat
      181 gagcagttgt tccttacccc agtgcatgtg cgggatgacg gcgtgggccg ccacgttcga
      241 ccagttcagg tgcgccagca gtgccgaaaa tacgtaggcc agaagtacca tcgccagcca
      301 tttgaggtag cgggcatagc gcacgtaggg agtaaaaatc tgcaacagca ggctgagcag
      361 cgcgaaggcg acaaccagcc accagaagcg gaggtgcgga ttaagcagtt ggactccctt
      421 ggccatggcc ccgagatcgg cgccgatgtt gaaggcgttt gccacgaaca gcagcagcgt
      481 acagatgtgc agagtacgcc tggaaaaatg taccctgatg tttgccgcca aaccgcggcc
      541 cgtgaccata ccgatacggg cgcacatttc ctggatcgca gccgccagcg ggtacaccca
      601 ggccgccagc cagagcagct ggtaaccgta gcgggcgccg gtctgagaat aggtagcgat
      661 accggaggga tcgtcgtcgg cagcgccggt ggtcaggccc gggccgagcg tgtgccagta
      721 atcctcggtt tttttaatca ctttgccggc cggcagcgcg gccttgacct gctggcccgc
      781 tacgatagta cggtccagca gtgctgccgg cgcttcggcg cttcggcgta ggaattcttc
      841 tgattttgcc atattggttt taacgcgtta ctgccttgat tgtatcattg tttggctgat
      901 agcctgctac tgtatcgccg gcactattcg gcgcagccgc cggtactgac ggcgtggtcc
      961 tgggcggccg cctggtttat ctcctgggcc acggccgccg tggtcagtgc gtagccgaca
     1021 tgattgtagg cggtggactg ggcaaataca acgccgacca cggcgccgtc ggccgtcacc
     1081 agcggaccgc cggaattgcc cggtatgata ttggcggcga ctgcgtacac atcgcgttcg
     1141 gtactgcccc ggtcataaat gttgcggccg gtggcagtga actcgtcgag tacggaagcc
     1201 ggctcggcgg tgaagccgcc gccgccggga tagccaagca ctgccgccgg agtgctttgg
     1261 ggcacgcggg agctattgat caggagcggg tcgccggcca gattggcgac ccgcagcacg
     1321 gcaaagtcca aatccgggtc aaaccagatg gcgacggcgg tgtgcgtgcc gttggcatct
     1381 ttgacgtacg ggtgggcgat accggcgacc acgtgagcat tggtagcgac caggttgttg
     1441 cccactacga agccggagcc ttcgacgata ccgccgcacc cctgcccctc tatcttgact
     1501 atcgatgctt tgtctttggc gaccgcagcg gcgagggcgc tcgaaggcgg caggttgatg
     1561 ttgtccggcg gcgtcggctc ggaaccgatg aaaacgttcg ggaagccgtt tggatctatc
     1621 aggtgtccga ggtcggcgat gatattcggc gccggcggca ggttactgtc cagcgccgtt
     1681 ataatgcgcg aggcgcgtat ggcattcttc agcgacggat acggcacact gctgaggata
     1741 gcggcaccga gccacacggc cagcagcacg gaggcggcgc tgagcaggga cccaaacagg
     1801 ttgtcggcct tgttcacggc tttttcctgc actttgtgct tcagaacgat gccaatatat
     1861 tccccgataa tgagtaacaa gaaggctgta ccgagcgtgg tcagcagcgt gaccagcgag
     1921 cgcgacagcg gactgtgtac cagcgatacg gtgtgcggct ctataaaggt gccgagcagc
     1981 aggccgccga aaaagccgac ggtggaaaac agctgctgca cgaaccctat ctcgcggccg
     2041 cgcaccagcg cgcttataag aaacaggacg atgaggatat ctactgtcat gttgtaagca
     2101 tcattatacg tgtttatgcg ttaatgctag cgccggctgc tgaaagttca gtgaaacttt
     2161 agcagcgtct caggatcact gtctttgggg tagtacacga gcggcggcac tcccttgctc
     2221 cattcgtcca ggaccggctg cag
//
LOCUS       DASSFD010000098         6086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1291631, whole genome shotgun sequence.
ACCESSION   DASSFD010000098 DASSFD010000000
VERSION     DASSFD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291631"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFH39_02220"
     CDS             complement(<1..299)
                     /locus_tag="VFH39_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924622.1"
                     /translation="MNVAIVGYGTEGRASCDYYLRQGAAVTVCDQNRDISVPAGVQTQ
                     LGDDYLHNLDRFDVIVRTAGMHPNVILGPNPGVADKITTQINEFLRVCPTKHVIGV"
     gene            complement(315..683)
                     /locus_tag="VFH39_02225"
     CDS             complement(315..683)
                     /locus_tag="VFH39_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6924623.1"
                     /translation="MPKIAIVEDDLAISQMYRFKFEAEGFEVETAENGKLGLELAKSM
                     KPDIILLDLMMPEMNGDEMLAALRKTAWGKGTKVVILTNKGEQEIPPEIKTLGVSAVI
                     LKADMTPRQVAEVVQKQLAA"
     gene            complement(729..1097)
                     /locus_tag="VFH39_02230"
     CDS             complement(729..1097)
                     /locus_tag="VFH39_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6924624.1"
                     /translation="MTKIALVEDDEAVSQLYQMKLHKEGYEVVLARDGAEGVQVVQST
                     QPDLVLLDLMLPQLSGDRVLAAIRQQPWGQNIKVIIMTNLTEDEAPKELHELGVSRFI
                     VKAQYTPTQLMEVIKQVLAE"
     gene            complement(1116..1565)
                     /locus_tag="VFH39_02235"
     CDS             complement(1116..1565)
                     /locus_tag="VFH39_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6924625.1"
                     /translation="MLPSSSLCNTTGVGKKSTTLTLKGCDISRILIVEDELPIQAMYR
                     YKLEAEGFEVHVASNGASGLEQTKKVSPDLILLDIRMPEMNGDEMLKLVREQEWGAEV
                     RVIVLTNLSKDEAPSILRLLGVDRYVVKAHYTPAQVVKIVREVLDGQ"
     gene            1716..3116
                     /locus_tag="VFH39_02240"
     CDS             1716..3116
                     /locus_tag="VFH39_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924626.1"
                     /translation="MTEQLTTPIQGFTGENQRRAQLPMDLWPAGVIVDRRKSIENGAS
                     AEPLVEASTAVDQAADEASQNRWQRIVRGAETGVAATVLLPSNEAIRAGAFAAGYSLM
                     PHSPAVGALTIGASTFALEAAGGLAAADLFKTERSARIMNWLRRRMKHTEDDEALSGA
                     SKMIATFMGGTVVGMALENYEDPDRTVAQNRRYSLKTSAWLGGVCAAVFGAGGTEALN
                     YTIDHPTVTGAVAGGAIATVEGGRRVASRLRVPKPVEDIQTEKWLDHDKHYEYRLVED
                     EEALHEVAELEQQVWDEKGFGNLTEEGYDKYNEHSRVFAAYDGDRCIGMTRMFKAEDG
                     IVPPFLDMEFYEDAEHEKLTASAVAGLTEELGTAAVLQEARGRRVNTRLWRQAYRDAV
                     LRGVKQWGIIMEPERVEKDLNGRYGFTFRQLGPAVDYQGGACAPFVMDLQEVSKSMQK
                     KKPLSHFWFVRKSLKA"
     gene            3144..5321
                     /locus_tag="VFH39_02245"
     CDS             3144..5321
                     /locus_tag="VFH39_02245"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6924627.1"
                     /translation="MLTIILSLTGLLLLVLSAVVLYSNYRSATNRWLAAFTLSGLLWL
                     LANLGANLSTTQHTNLMFSRAALIGAALLPFAYLLFCVHFTKAEVRMPARIGLFLPIA
                     LLLLSVPTSYNIVRVTGQNFAIQPGIAYAVLLLVLVVYFSYGMHLLLADYRRAKLARR
                     QQLYYILFGTVLTVVPGFILSGLLPLLDIAAGTTAAPVVSAFFSIFTALAIIKHQLLD
                     VRLVIARSLAYAGSLVVIASVYGVVVFGTAQFIFGLHFSIASQIYISAATGLAVLTFG
                     GVKRNFDRWSNRLFYRDAYDTQALFDNLNQTLVASLDLTRLMTDISTLLAKTFKADYC
                     LMGLKESIGTPQRIVGSEKRDVSFKDIMALRKHTVAYHRQVIVTDDLEDKKFAELKRV
                     LARNNIAVLIRLAPNVQRSEEGLGYVALGNKKSGNPYSQQDIRVLETVANELIIAVQN
                     ALHFEEIQNFNTTLQEKITDATRQLRRTNEKLKALDETKDDFISMASHQLRTPLTSVK
                     GYVSLVLDGDAGPLKPMQRELLTQAFASSQRMVYLISDLLNVSRLRTGKFVIDPAPVN
                     LADMIEEELAQLKEAAETRSVELSYSKPAHFPTLMLDETKTRQVIMNFVDNAIYYTPA
                     GGHIKVELADKPHGIELKVVDDGIGVPKHEQHHLFTKFYRAGNARKARPDGTGLGLFM
                     AKKVVVSQGGSIVFDSQEGKGSTFGFTFAKAGHVPENTPAITP"
     gene            5470..>6086
                     /locus_tag="VFH39_02250"
     CDS             5470..>6086
                     /locus_tag="VFH39_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924628.1"
                     /translation="MKVLKQLLGRFSRTKVKKVAATAAVFVLVSGATAVGVSAVSGKS
                     TDAAVSACGANSSNSIIRNGASSRKAFIADVKKSSELQAFYKNFGLSPNDYNRFVSDA
                     KPGVAYDNNTIVVDGNVVANSVGSTGRLQGCQGSNPRVFHIAGKNFYGNMNSKAFASG
                     THAIPVDVLFDGNGKIQFAVMTSVCGNATYQHPVPPTMECKELHA"
BASE COUNT         1303 a         1678 c         1721 g         1384 t
ORIGIN      
        1 acccctatga catgcttcgt cgggcaaacc ctcaggaact cgttgatctg ggtagtgatt
       61 ttatcggcca cgcccgggtt tggccccaga atgacgttcg ggtgcatgcc ggccgtccgg
      121 acgataacgt cgaaccggtc caggttgtgc aagtagtcat cgccgagctg tgtctggacg
      181 ccagccggga cgctgatgtc acggttctgg tcacagacgg tcacggccgc gccctggcgc
      241 aggtaatagt cgcatgaggc ccgaccttcg gtaccgtatc ctacgatggc tacgttcata
      301 cgtccgctga gcctctacgc cgccagctgt ttctgtacca cttcggccac ctggcgcggc
      361 gtcatgtcgg ccttcaatat gacggcgctg acgccgagcg ttttgatctc cggcggaatc
      421 tcttgctcgc ccttgttggt cagaatcacc accttggtgc ccttgcccca ggccgtcttg
      481 cgcagggcgg ccagcatctc gtcgccgttc atttctggca tcatcaggtc cagcaggatt
      541 atgtccggct tcatgctctt ggccagctcc aggccaagct taccgttctc ggcggtttct
      601 acttcgaagc cctcggcttc gaacttaaag cggtacatct ggcttatcgc cagatcatcc
      661 tcgactatgg ctatcttagg catgacccca tcatacggga cagcccgcgg ccttgacaac
      721 acgccggtct attcggccag cacctgcttg atgacttcca tcagctgcgt cggggtgtac
      781 tgggctttca cgatgaaccg gctgaccccc agctcgtgca gctccttggg ggcttcgtct
      841 tcggtcaggt tggtcatgat aatcaccttt atgttctgcc cccagggctg ctggcggatg
      901 gcggccagca cccggtcacc gctcagctgc gggagcatca gatccagcag caccagatcg
      961 ggttgggtag actgtaccac ctgcacccct tcggcaccgt cgcgcgccag caccacctca
     1021 tagccttctt tgtgcagttt catctggtac agctgcgaaa ctgcttcgtc gtcttcgacc
     1081 agggcgattt tcgtcataat tcttatacta ccaccctact ggccgtccag cacttcccga
     1141 acgattttca ccacctgcgc gggggtgtaa tgcgccttta cgacgtagcg gtccactccg
     1201 agcagccgca gtatgctggg ggcttcgtcc ttgctcaggt tggtcagcac tatcacccgc
     1261 acctcggccc cccactcctg ttcgcgcacc agcttcagca tctcgtcgcc gttcatttcg
     1321 ggcatgcgta tatccagcag tatcagatcg ggcgagacct tcttggtttg ctccagcccg
     1381 ctggcgccgt tgctggccac gtgcacctcg aacccttcgg cctccagctt gtagcggtac
     1441 atggcctgga tcggtagctc gtcttcgaca atcagaatgc gggaaatatc acaacctttt
     1501 agcgttaacg ttgttgactt cttacctacc cctgtagtat tacataagct agagcttggt
     1561 aacaagcaag tatattagga gcttaatcag acgtcacttg acccctgtag ggccgtcagg
     1621 tgacgtcttt ttatttacca ctagtatatt gcaaatttat caaacttatg ctaatataag
     1681 caaattacca ttttaaccat aggcattttc attttatgac cgagcaatta actactccca
     1741 tccagggatt caccggcgaa aaccaacgcc gtgcgcagtt gcctatggac ttatggcccg
     1801 caggcgtcat cgttgaccgc cgtaaatcta ttgaaaacgg agcaagtgct gagccgttgg
     1861 tcgaagcctc aactgccgtt gaccaggcgg ccgacgaagc atcccagaac agatggcaac
     1921 gtattgtacg gggggcggaa acaggtgtgg ctgctaccgt actgctaccc tccaatgagg
     1981 ccatacgtgc cggtgccttt gctgcaggct atagtttgat gccgcatagc ccagcggtcg
     2041 gtgcactgac tataggggcc tctacttttg cgctggaagc cgccggtgga ctggctgcag
     2101 ccgacttatt taagacagaa cgtagcgcac gaatcatgaa ctggcttcgt cggcgtatga
     2161 agcacacaga agacgatgag gctctttctg gagcgtcaaa gatgattgct acctttatgg
     2221 gtggcacggt tgtgggcatg gccctcgaga attacgagga cccggatcgt acagttgccc
     2281 agaaccgtcg ctatagcctt aagacttccg catggttagg cggggtgtgc gctgctgtct
     2341 tcggcgctgg tggcacagaa gctcttaatt atacgattga tcatccaacc gttaccggtg
     2401 cggtggcggg gggtgccata gctactgttg aaggtggtcg ccgggtggct tccaggctac
     2461 gggtgccaaa accggtcgaa gatatccaga ctgaaaagtg gctcgatcac gacaaacact
     2521 acgagtatcg tttagttgag gatgaagaag ctttgcatga agtcgctgag cttgaacagc
     2581 aagtgtggga cgaaaaaggc ttcggtaatc tgaccgaaga aggttatgac aaatataacg
     2641 agcacagccg ggtgttcgct gcgtacgatg gcgatcgctg tataggcatg acccgcatgt
     2701 tcaaagccga agacggcatc gtgccgccat tccttgatat ggaattttac gaggacgctg
     2761 aacacgagaa gcttactgcc tcggcggtgg ctggacttac ggaagagttg ggcacggcag
     2821 cagtgttgca agaagctcgc ggacgtcgag ttaacacgcg gctgtggcga caggcatatc
     2881 gtgacgccgt cctacggggc gtcaagcagt ggggtatcat catggagccg gaacgcgtag
     2941 agaaggacct gaacggcagg tatggcttta cgttccgtca gcttgggccc gctgtggact
     3001 accagggcgg tgcatgtgcc ccgttcgtca tggatctgca agaagtctct aaatctatgc
     3061 agaagaagaa gccgctgagc catttctggt tcgtccgcaa atcgcttaaa gcgtaaccgt
     3121 tgtgccgtat aatggtaggc gaaatgctta ccattatact gtcgctgacc gggctgctgc
     3181 tgttggtgct gtcagctgta gtgttgtatt cgaactaccg gagtgcaacg aaccggtggc
     3241 tggcggcgtt tacgctaagt ggtctgttgt ggctgctagc gaacctaggc gctaatctct
     3301 ctacaacaca gcatacgaac cttatgttct cgcgggcagc cctcattggg gcggctttgc
     3361 taccgttcgc ttatttgctg ttctgtgtgc acttcacgaa ggctgaggtt agaatgcctg
     3421 cgagaatcgg tctcttcttg ccgattgcct tgctgctgct gagtgtacct acctcgtaca
     3481 acattgtgag agtgaccggg cagaactttg ccattcagcc aggtatcgcc tatgcagtac
     3541 tgctgctggt gttggttgtt tacttcagct acggtatgca cttgctactg gcggattacc
     3601 ggcgggcaaa gctggcgcgt cgtcagcaac tgtactacat actgttcggt acggtattaa
     3661 cggttgtacc aggctttatc ctgagcgggt tgctgccttt attggacatt gctgccggca
     3721 ccactgcggc accggtcgtc tcggctttct tctcgatatt taccgcctta gcgattatca
     3781 aacaccaact tctcgatgtg cgactggtca tcgcccgttc actggcatac gccggttctc
     3841 tggttgtaat tgcgtcggtg tatggagtag tggtcttcgg caccgcacaa tttattttcg
     3901 ggcttcactt ctctattgct agccagatat acatttcggc ggctaccggg ctggccgtgc
     3961 tgacgttcgg tggggttaag cggaacttcg acagatggag caaccggctg ttttaccggg
     4021 acgcctacga tacgcaggcc ctgttcgata accttaacca gacattggtg gcatcgctgg
     4081 acctgacacg gttgatgacg gatatatcga cgctgctggc caagactttc aaggcagact
     4141 actgtctgat ggggctgaag gaaagcatcg gcacgccgca acgcatcgta ggcagcgaga
     4201 agcgcgatgt gtcgttcaag gacatcatgg ccctgcgcaa acacaccgtg gcctaccacc
     4261 gccaagtgat cgtgactgac gatttggaag ataagaaatt cgccgagctg aagcgcgtcc
     4321 tggcgcgcaa taacatcgcg gtgctgatac gcctggcgcc gaacgtccag cgcagcgaag
     4381 aagggctggg ctacgtggcg ctcggcaata aaaagagcgg taacccgtac agccagcagg
     4441 acatccgggt gttggagacg gtggcgaacg aactgattat cgccgtgcag aacgcgttgc
     4501 acttcgagga gatccagaac ttcaacacca ccctccagga gaaaattacc gatgcgacca
     4561 ggcagttacg gcgcaccaac gagaagctga aggcgctgga cgagaccaag gacgacttta
     4621 taagtatggc cagccaccag ctgcgcacgc cgctgaccag cgtgaagggc tacgtgagcc
     4681 tggtgttgga cggcgatgct gggccgctaa agccgatgca gcgcgagctg ctgacgcagg
     4741 ccttcgccag ttcgcagcgc atggtgtacc tcatatctga cctattgaat gtatcgcggt
     4801 tgcgtaccgg caagttcgtg atcgatccgg caccggtgaa tctggcggac atgatcgagg
     4861 aggagctggc gcagctgaag gaagcggccg agacgcggtc ggtggaactt agctacagca
     4921 agccggcgca cttcccgacg ctgatgttgg acgaaaccaa gacccgacaa gtgattatga
     4981 acttcgtcga taacgccatt tattacacac cagccggggg ccacataaaa gtagagctgg
     5041 ccgacaaacc gcacggaatt gagctgaagg tggtggatga cggcatcggt gtgccaaagc
     5101 acgagcaaca ccacctgttt accaagttct atcgggccgg taacgcccgc aaagcccggc
     5161 cggatggtac ggggctagga ctgttcatgg ccaagaaagt cgtggtgtcg caaggcggct
     5221 ccatagtatt tgacagtcag gaaggcaagg ggagtacgtt cggcttcacc ttcgccaagg
     5281 ccgggcatgt gcccgaaaac acaccagcaa taactcctta aacaaacgct acggtaagat
     5341 tttggacaat acgcaccctt ggggcggtgc tacactgccg ccgtaaggtg cttatggttt
     5401 tatgtcataa tattgacatt agcgccttca tgtgatatta tgcattgcat acatcaggga
     5461 ggtgttgtaa tgaaggtatt aaagcaactg ttggggcggt tcagccgcac caaggtcaag
     5521 aaagtcgctg ccaccgcggc agtgttcgta ctggtttccg gggctaccgc cgtcggtgtc
     5581 tcggctgtca gtggtaagtc caccgacgct gctgtcagcg cctgtggtgc caactctagt
     5641 aactcaatca tccgcaacgg cgccagcagc cgcaaagcgt tcatcgcgga tgttaagaag
     5701 agctctgaac tccaggcctt ctataagaac ttcggcctga gcccgaatga ctacaaccgc
     5761 ttcgtgagcg acgccaagcc gggcgtggcc tacgacaaca acaccatcgt ggtcgatggt
     5821 aacgtcgtgg ccaacagtgt cggcagcacc ggccggctgc agggctgcca gggtagcaac
     5881 ccgcgggtgt tccacattgc cggtaagaac ttttacggca acatgaacag caaggctttc
     5941 gccagcggta cgcacgccat tccggtcgac gtcctgttcg acggtaacgg caagatccag
     6001 ttcgccgtca tgacaagcgt ctgcggcaac gccacctatc agcacccggt tcctcctact
     6061 atggagtgta aagagctgca cgccaa
//
LOCUS       DASSFD010000099         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1653189, whole genome shotgun sequence.
ACCESSION   DASSFD010000099 DASSFD010000000
VERSION     DASSFD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653189"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /gene="dnaE"
                     /locus_tag="VFH39_02255"
     CDS             <1..1693
                     /gene="dnaE"
                     /locus_tag="VFH39_02255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6924629.1"
                     /translation="PSVFEDIVAMVALYRPGPMQFIDDFIARKRGEKPITYFHDAMEN
                     ALKNTYGVLVYQEQVMQISKEVCGFTGGEADTLRKAIGKKKIDMMRKMKARMIEGAQE
                     VSGIDAATMERFWKQLEDFAAYCFNKSHAACYGLIAYQTAYLKAHYPAAYMAALMTSD
                     FDDTDRLAIEITECQHMGIQVLLPDVNESFVEFAVVKSPKGVTSPLAPVRFGMAAIKN
                     VGTGAVEEILRARAEAPFESLEDFLSRVSCRIVNRKALESLIKTGAFDRFGDRMVLLH
                     NIDLMLAYAQRLQKQQSSGQTDIFGNSVEEHLKPVLTLETAGVSHDQHEQLLWERELL
                     GLYLSKHPLDLFENILSEQTMPLKELKGEHDGKPVTVGGAIIDIREIVTKNGQKMAFV
                     KLEDRSGEAEAILFPSSYQQTLGVWVRDKVVLIRGRVNGKDREGRLSDEAKIMVDDAR
                     EITVAQAEAYQPTGKKKKVPKASPKAKQAAAAAQPSVTAPAKVYIRLSSSNDEQTLVT
                     LKQTLDSYRGDTDVVLVLGEADKKQAIKLPDGINKDSEGLEVLRELVGTDNLVVR"
     gene            complement(1695..2753)
                     /locus_tag="VFH39_02260"
     CDS             complement(1695..2753)
                     /locus_tag="VFH39_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6924630.1"
                     /translation="MTLVARAKPPAHHKKRSGSHHRRTKSYHKAYWPYLPIALIVLGG
                     IIFNALVPAHKAVLGYATDMSISSLLSDTNAQRSDNGLPGLGLNSQLDQAAQAKANDM
                     AARDYWSHNTPDGQTPWSFIIAAGYNYQTAGENLAYGFDSASETVTGWMNSPEHRANI
                     LNTTYKEVGFGVANSANYQGTGPETIVVAMYAEPVGYVAPAPAPAVAQSTPATRPVPA
                     PTPITSAPASQAAPATQNQPVTGQASNADAGAPVKLPKTPVVTTTPLKPVTETKSQEV
                     SSVALLTAGKAPWSLTLLSVVLAVSFLLFFMRHTLAWHKVLRRGERFVIKHPALDIAF
                     AFIIVAGLLLSRTVGSIR"
     gene            complement(2794..>3144)
                     /locus_tag="VFH39_02265"
     CDS             complement(2794..>3144)
                     /locus_tag="VFH39_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET6924631.1"
                     /translation="PHLARKIHQQISKTALGAESSQSWQRRDDIAPLLDELRSAGFSI
                     AALEQSTDSIELPVYHAPDKLALLVGREVEGLESEVLAACDLALEIPMFGAKESFNVV
                     QASAMALYHCRFMP"
BASE COUNT          652 a          897 c         1031 g          564 t
ORIGIN      
        1 gccgtcggtg ttcgaggaca tcgtggccat ggtggcgctg taccggccgg gaccgatgca
       61 gttcatcgat gacttcatcg cccgcaagcg cggcgaaaag cccatcacct acttccacga
      121 cgccatggag aatgcgctga agaacaccta cggcgtgctg gtgtaccagg aacaggtgat
      181 gcagatttct aaggaagtct gcggctttac cggcggtgag gccgacacgc tgcgtaaggc
      241 catcggcaag aagaagattg acatgatgcg caagatgaaa gcgcgcatga tcgagggcgc
      301 ccaggaagtg tccggcatcg atgccgctac catggagcgc ttctggaagc agctcgaaga
      361 cttcgcggcc tactgcttca acaagtcgca cgccgcctgt tacggtctga tcgcctacca
      421 gacggcctac ctgaaagcgc actacccggc ggcctacatg gcggcgctga tgaccagcga
      481 cttcgacgac accgaccgcc tggccataga gatcaccgag tgccagcaca tgggcatcca
      541 ggtgctgctg ccggacgtga acgaatcgtt cgtggagttc gccgtggtca aaagcccgaa
      601 gggcgtgacg tcgccgctgg cgccggtgcg cttcggcatg gcggccataa agaacgtcgg
      661 caccggcgca gtcgaagaga tattgcgcgc ccgggccgag gcgccgttcg agagtctgga
      721 agatttcctg agccgggtca gctgccgcat cgtcaaccgc aaggcgctgg aaagcctcat
      781 aaaaaccggc gcgttcgacc gcttcggcga ccgcatggtg ctgctgcaca acatcgacct
      841 gatgctggct tacgcccagc ggctgcagaa gcagcagtcc agcggccaga ccgacatctt
      901 cggcaacagt gtcgaggagc acctgaagcc cgtgctcacg cttgaaaccg ccggcgtatc
      961 tcacgaccag cacgagcaac tgctgtggga acgcgaactg ctgggcttgt acctgagcaa
     1021 gcatccgctg gacctgttcg aaaacattct gagcgagcag accatgccgc tgaaggagct
     1081 gaaaggggag cacgacggaa agccggtgac ggtgggcggc gcaatcatcg atatccgcga
     1141 aatcgtcacc aagaatggcc agaagatggc cttcgtgaag ctggaagacc gcagcggcga
     1201 ggccgaagcg attctgttcc cgagcagtta ccagcagaca ctcggcgtct gggtacgcga
     1261 caaagtggtg ctgatacgcg gccgggtcaa cggcaaggat cgcgaaggcc gcttgagcga
     1321 cgaagccaag atcatggtcg acgacgcccg cgagataacg gtcgcccaag ccgaggctta
     1381 ccagccgacc ggcaagaaga aaaaggtgcc caaggccagc ccgaaagcca agcaggccgc
     1441 cgcggcagcg cagccgtcgg tcacggcacc ggccaaggtg tacatccgtc tcagcagcag
     1501 taacgacgaa cagaccctgg tgacgctgaa gcagacgctg gacagctacc gcggcgatac
     1561 cgatgtggta ttggtactcg gcgaggccga caaaaagcag gccataaagc tgccggacgg
     1621 catcaacaaa gacagcgaag gcctggaagt cctgcgcgaa ctggtcggta ccgacaatct
     1681 cgtcgtccgc tagcttagcg gatggagccg acggtacggc tcagcagcag accggcgacg
     1741 atgatgaagg cgaaggcgat atccagcgcc gggtgcttta tgacgaagcg ctcgccgcgg
     1801 cgcagcactt tgtgccaggc aagggtgtgg cgcatgaaga acagcaggaa gctgacggcg
     1861 agcaccacgg acagcaaggt gaggctccac ggcgctttgc cggcggtcag caaggctacc
     1921 gacgaaactt cttgcgactt ggtttcggtg accggtttca gcggcgtggt cgtaaccaca
     1981 ggcgtcttgg ggagcttcac cggcgcgccg gcatcggcat tgctggcttg acccgtcacg
     2041 ggctggttct gggtggcagg tgctgcctgg gacgctggtg cgctagttat cggtgtcggt
     2101 gcgggcaccg gtcgggtggc cggtgtactt tgggcgacgg ccggggcggg agcgggcgcg
     2161 acgtagccga ccggttcggc atacatggcg accacgatgg tttccgggcc ggtgccctgg
     2221 taattggcgg aattggcgac accgaagccg acctctttgt aagtagtgtt caagatgttg
     2281 gcgcggtgtt cggggctgtt catccagccg gtaaccgtct cgctggcgct gtcgaaaccg
     2341 taggcgaggt tctcgccggc cgtttgatag ttgtagccgg cggcaatgat gaagctccag
     2401 ggcgtctggc cgtcgggcgt gttgtgcgac cagtagtcgc gggcggccat gtcgttggct
     2461 ttagcctggg cggcctgatc cagctgcgaa ttaagtccga gaccaggcag gccgttgtcg
     2521 ctgcgctggg cattggtgtc gctcagcagg ctggaaatac tcatatcggt ggcgtaaccc
     2581 aacaccgctt tgtgcgccgg caccagtgcg ttgaagatga taccgccgag cacgatgagg
     2641 gcaatcggca ggtatggcca atacgccttg tggtaagact tggtgcgacg gtggtggctg
     2701 ccggagcgct tcttgtgatg cgccggcggt ttggcgcggg ctacgagtgt catatgtttg
     2761 ttttatctga ttataggtgg cattataaca tgcttatggc atgaaccggc aatggtatag
     2821 cgccatggcc gaagcctgca ccacgttgaa ggattctttg gcgccgaaca tcggtatctc
     2881 gagcgccaga tcgcaggccg ccagcacttc cgactccaaa ccttccactt cgcgtcccac
     2941 taagagagcc aatttatctg gagcatgata caccggcagt tcgatgctat ctgtcgactg
     3001 ctccagcgcc gcaatggaga acccggctga gcggagctca tctagcagtg gtgcgatgtc
     3061 atcgcgacgc tgccaagact ggctgctttc cgctcccaag gccgtcttgc tgatctgctg
     3121 atgaattttg cgagccaggt gcgg
//
LOCUS       DASSFD010000100         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1418769, whole genome shotgun sequence.
ACCESSION   DASSFD010000100 DASSFD010000000
VERSION     DASSFD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418769"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFH39_02270"
     CDS             <1..391
                     /locus_tag="VFH39_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6924632.1"
                     /translation="ESHDLMPYEALLQGSNAGNISAVMLGNMIIPGLTGGEPASISPA
                     AVKLVRDMGYNGLVTTDALSAIGYDGMSLSQNVARAVEAGVDAPLFSYSSEQAILRAI
                     HNISRNVSSSQINASYAKVAALKQNAP"
     gene            434..>2542
                     /gene="polA"
                     /locus_tag="VFH39_02275"
     CDS             434..>2542
                     /gene="polA"
                     /locus_tag="VFH39_02275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET6924633.1"
                     /translation="MSEAKSPKKFAIIDGKSVFYRGYYAMPNLATKDGTPTGGVFGFA
                     TMALEVIRKLKPDYVAVAWDKPKTNIRKRLELYPEYKAGRKPAPADFYDQIPVLHELL
                     DAFGWPLYELDDYEADDIMGTLAVQASAKDIDTMLITSDLDMLQLVNPHVKVFALKKG
                     LSNIELYSPESFTAKYGIDVHQFLDLKALKGDSSDNIPGVPGIGEKGAIDLLKQYQTL
                     DGVYDNLGLLRDSLRQKLEAGKDLAYLSKELASIWTDAPLKLDLQEVDGTKVKAEKLL
                     ELLNKLEFRSLARQLPEVMQVAIENHHSTNGTSSPKPGKNVLIDSDDKLTEVKLSADK
                     PVYVHSRCAGKHGRNPQVLSLSSDGKTAYSIDLTKVSAEPVANLLAGVDSLVGYDVKY
                     TLKVLIDLGMTKLPAVGHDVLVGAFLINSLRREQTLTELAENELSYDGSSFEDLDAEE
                     VMSRAPEIIAVIKSLYDRQAKELADIPKLPELARDVEWPVIPVLARMEYEGIELDTKY
                     LAKFAEEIDDLVSDYEQQIYGYADQEFNIGSPSQLAEVLFDKLKLPTQGIKKGKTGYS
                     TAASELDKLRGQHEIIDLITQYREVAKLKNTYVDTLPKQVDEHSRIHTTFNLTIAQTG
                     RLSSVDPNLQNIPTRTDLGRRVRTAFVAGKGKKLVSADYSQFELRLAAYLADDKELID
                     MFNRGADVHTATAAQVYGREL"
BASE COUNT          598 a          753 c          745 g          446 t
ORIGIN      
        1 ggagtcccac gacctgatgc cgtacgaggc acttttgcag ggctcaaacg ccggcaatat
       61 ttcggcggtt atgctcggca atatgattat tcctggcttg accgggggtg aaccggccag
      121 tatcagcccg gcggcggtca aactggtgcg cgatatgggg tataacggct tagttactac
      181 cgatgcgctg tcggctatcg gttacgatgg catgtcgctt tcgcagaacg tcgcccgtgc
      241 cgttgaggcc ggcgtggatg ctccgctgtt ttcgtactca agtgagcagg caatcttacg
      301 ggctatccac aacatcagtc gtaacgtctc cagtagccag atcaatgctt cgtacgccaa
      361 agtggccgct ctgaagcaga acgctccgta agcgtacgtt ccggcaagcc gtaatctgtt
      421 ctatacttaa accatgagtg aggccaaatc gcctaagaag ttcgccatca tcgacggcaa
      481 gtcggtcttt taccgcggtt actacgccat gcccaacttg gcgaccaagg acggtacgcc
      541 gaccggcggt gtgttcggtt tcgccaccat ggcgctggag gtcatccgca agctgaagcc
      601 ggactacgtg gcggtcgcct gggacaaacc gaagaccaac atccgcaagc ggctggagct
      661 gtacccggag tacaaggccg gacgcaaacc ggccccggct gacttctacg accagattcc
      721 ggtgctacac gagctgctgg acgccttcgg ctggccgcta tatgaactgg acgactacga
      781 agccgacgac atcatgggca cgctggcagt gcaggcctct gccaaagaca tagacaccat
      841 gcttataacc agcgatctcg acatgctgca gctggtgaac ccgcacgtta aggtgtttgc
      901 gctcaagaag ggcctgagca acatagaatt gtactcacct gaaagcttca ccgccaagta
      961 cggcatagat gtgcaccaat ttttggacct gaaggcgctc aagggcgaca gttcggacaa
     1021 cataccaggg gtgccgggca tcggcgagaa gggggcgatc gatttactca agcagtatca
     1081 gacgctcgat ggcgtgtacg acaatctcgg actgttgcgg gacagcctgc gccagaaact
     1141 cgaagccggc aaagacctgg catacttgag caaagagctg gccagcatct ggaccgacgc
     1201 gccgctcaag ctggacctgc aggaagtcga tggcacgaag gtgaaggcag agaagctgct
     1261 ggaactgctg aacaaactgg aattccgcag cctggcccgg cagctgccgg aggtgatgca
     1321 ggtggcgatc gagaatcacc acagcaccaa cggtacgagc tcacccaaac caggtaaaaa
     1381 tgtgcttatc gacagcgatg acaaactcac ggaggtcaag ctgtcagccg acaagccggt
     1441 atatgtgcac tctcgttgcg ccggtaagca cggtcgcaac ccgcaggtac tgagcttgag
     1501 cagcgatggc aaaacggcgt atagcatcga cctgacgaag gtgagtgccg aacctgtggc
     1561 gaatttgctg gccggtgtcg actccctcgt cggctacgac gtgaagtaca ccctcaaggt
     1621 actcatcgac cttggtatga ccaaattacc ggcggtcggg cacgatgtgc tggtcggcgc
     1681 gttccttatc aactcattgc ggcgcgagca gacgctgacc gaactggccg aaaacgaact
     1741 gagctacgat ggctcctcgt tcgaagacct tgatgccgaa gaagtcatga gccgcgcacc
     1801 ggaaatcatc gccgtcataa agtcgctgta cgaccggcag gccaaagagc tggccgacat
     1861 tccgaagctg ccggagctgg cccgcgacgt cgaatggccg gtcatcccgg tgctggcgcg
     1921 catggagtac gaaggcatcg aactggacac gaagtacctt gctaagttcg ccgaggaaat
     1981 cgacgacctg gtgtccgact acgagcagca gatctacggc tacgccgacc aggaattcaa
     2041 catcggcagc cccagccagt tggccgaggt gttgttcgat aagctcaagc tgccgaccca
     2101 gggcatcaaa aagggcaaga ccggctacag caccgctgcc agcgagctcg acaagctgcg
     2161 cggccagcac gagatcatcg acctcataac ccagtaccgc gaggtcgcca agctcaagaa
     2221 cacctatgtc gatacgctgc ccaagcaggt cgacgagcac agccgcatcc acaccacctt
     2281 caacctgacc atcgcgcaga ccggccggct cagcagcgtc gacccgaacc tgcagaacat
     2341 cccgacccgc accgacctcg gccggcgggt gcggacggcg ttcgtggcgg gcaagggcaa
     2401 gaagctggta agtgccgatt acagccagtt cgagctgcgc ctggcggcct acctggctga
     2461 cgataaagag cttatagaca tgttcaaccg cggcgccgac gtgcacaccg ccactgcggc
     2521 ccaggtgtac ggccgcgagc tc
//
LOCUS       DASSFD010000101         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_278585, whole genome shotgun sequence.
ACCESSION   DASSFD010000101 DASSFD010000000
VERSION     DASSFD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278585"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFH39_02280"
     CDS             <1..431
                     /locus_tag="VFH39_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET6924634.1"
                     /translation="RSAIISIVVAVVVLAGGGVWLASRHKSNTTNTNTASMNMNTQTT
                     TDSNNLNPTATNQVTIENMAFTPANISISSGSTVTWTNKDSVTHTVTETDGQNGPSLT
                     VEPGKSVVFTFDKPGIYRYHCTIHPSMTGTVTVTAAGSGS"
     gene            complement(850..1770)
                     /gene="holA"
                     /locus_tag="VFH39_02285"
     CDS             complement(850..1770)
                     /gene="holA"
                     /locus_tag="VFH39_02285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET6924635.1"
                     /translation="MIITLTGENAFGLRGELDGLVAGFVAEQGELGVERLDGETVTFA
                     RISEALTSLPFLMSKKLVLISRGSANKQFAEQTQDVLNNLPETTDVILVEPKLDKRSG
                     YYKFLKKSTDFREFNPLDVNGLSRWLVSEAKARGGSLSQRDAQYLVERVGLNQQLLAN
                     ELEKLLLYEPQVSRESIELLTEPTPQSTVFQLLEAAFAGNGKRAMQLYREQRAQKVEP
                     PQIVAMLAWQLHVLALIKTAGERSGDAVASEAHLSPYVVRKSQAIANKLSYAELKELV
                     RELADIDVRSKREALNVDDALQNYLLKLAL"
     gene            complement(1790..>2572)
                     /locus_tag="VFH39_02290"
     CDS             complement(1790..>2572)
                     /locus_tag="VFH39_02290"
                     /inference="COORDINATES: protein motif:HMM:NF037672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HET6924636.1"
                     /translation="VGQWFRDYMGGTRHRYPRHEMEFHGFNLTTTDGPKDLLHSNVKV
                     AGIRPNIHVQGGLTSNFIQWRSSAYVEAAGRGEKPDLVKRIYLNPTMESSVKIFTEIV
                     DAADKAGLQVKGKILDRSTSAVSSFTFKKDGSIGSLRGDGIVIGTSAKDANSLLGIVE
                     EIYNKHRAAFAGRKVPRVPKQIGQGVAIGEDISDTESLTSHRAAVIGNAALLAKEKLG
                     LQQWEKVSPVQEAKAIQTFRKLWSQVAAANGIDPQNIAFNRR"
BASE COUNT          554 a          827 c          630 g          561 t
ORIGIN      
        1 gccgatccgc gattatcagc atagtggtgg cagtggtcgt cctggccggc ggcggcgtct
       61 ggctggcgtc acgccacaaa tccaacacta ccaacaccaa caccgccagc atgaacatga
      121 acacccagac cacgaccgac agcaacaacc tgaacccgac ggccaccaac caggtgacca
      181 tcgagaacat ggccttcacg ccggctaaca tcagcatttc ttccggcagc accgtaacct
      241 ggaccaacaa agacagcgtt acgcacaccg ttaccgagac cgacggccaa aacggcccgt
      301 cccttaccgt ggagcctggc aaaagcgtcg tcttcacctt cgacaagccc ggcatctacc
      361 gctaccactg cacgatccac ccgagcatga ccggcaccgt cacggttacc gccgccggca
      421 gcggcagcta gccgcctgga atctagaatc tagaatttag aacagattag agaaggtaga
      481 agctggaagc tagatactcg aacccagagg gccatctagc acttcccgca gcccggccct
      541 aggagcgaac ccggctcagt ccgttcgtga cctttacaac ttccgggagg gcggtcggca
      601 gtgtctgagc cggaggcgag tttgccgacc ggttgtaaag agtcacaacg gctgaccgag
      661 tgagcggatc gggccttgat tttttgccta cttttgcatc aaggcaaaag tagagggtga
      721 gccgctaaca gcggctcagg agacagagag gttcggtttc agcctagctg gccccgtcaa
      781 aacaacgtct gtagcccacg tagcggcccc aggacaggcc gcgccagagg agcagtatcc
      841 gatacgaccc tacagcgcca gcttcaacag ataattctgc agcgcatcat ccacattaag
      901 cgcctcgcgc ttgctccgca cgtctatgtc ggccaactcc cgcaccagct ctttcagctc
      961 cgcatagctg agcttgttgg ctatggcctg gcttttgcgt accacgtacg ggctcaggtg
     1021 agcctcgctg gcaacggcat cgccgctgcg ctccccggcc gtctttataa gcgccagcac
     1081 gtgcagctgc caggccagca tggccacgat ctgtggcggc tccaccttct gcgcccgctg
     1141 ctcgcgatac agttgcatgg ctcgcttgcc gttgccggcg aaggccgcct ccagcagctg
     1201 gaacacggtg ctctgcggcg tcggttcggt caaaagctca atactctcac ggctcacctg
     1261 cggctcgtac agcagtagct tctccagctc gttggccaat agctgctggt tcaggcccac
     1321 ccgctccacc aagtactgcg catcacgttg gctgagactg ccgccgcgcg ccttcgcctc
     1381 gctcaccagc caacggctca gaccgttcac atccagcggg ttgaactccc ggaaatcggt
     1441 cgatttcttc aaaaatttgt aatagccgga gcgcttgtcg agcttcggtt cgaccaaaat
     1501 aacatcggtc gtttccggca aattgttcaa cacatcctgg gtttgctcag cgaactgctt
     1561 gttggcgctg ccccggctta taagcacaag ttttttgctc ataaggaacg gcaggctcgt
     1621 caaagcctca ctgatccgcg cgaacgtcac agtctcgccg tccagccgct ccacccccag
     1681 ctcgccctgc tccgccacga acccggccac caaaccatcg agctccccgc gaagcccgaa
     1741 cgcgttctct cccgtcagcg ttattatcac atttctattc tacagggaac tagcgcctat
     1801 tgaacgcgat attctgcggg tctatgccat tggcagcagc aacctgcgac cagagcttgc
     1861 ggaaagtttg tattgcttta gcctcctgca ctggcgatac cttctcccat tgctgtagac
     1921 caagtttttc cttcgccagt agcgcggcat tgccgattac tgcggcacgg tggcttgtca
     1981 aagattcagt gtctgaaata tcttcaccaa tagctactcc ttgtcctatc tgcttaggaa
     2041 cccgcggcac ctttcggccc gcaaatgcag cccgatgctt gttatagatt tcctctacga
     2101 tacccagtag actattggcg tctttggcgc tcgtgccgat aacgatgcca tcacccctca
     2161 ggctgcctat gctcccgtcc tttttgaagg taaaactact cactgcgcta gtggaacgat
     2221 caagtatctt gccctttacc tgtaaccctg ccttgtcggc agcatcgacg atttcagtaa
     2281 agatcttgac acttgattcc atcgttgggt tcaaatagat tcgctttacc aaatcgggct
     2341 tctcgcccct tcctgccgct tctacgtagg cacttgagcg ccactggatg aaattacttg
     2401 ttaatcctcc ctgcacatgg atattaggac gtatccccgc aaccttaaca ttgctgtgca
     2461 ataaatcttt tgggccatcc gtcgtagtga gattaaagcc atggaattcc atctcatgcc
     2521 gtgggtacct atgccgcgta ccacccatgt aatcacggaa ccattggccg ac
//
LOCUS       DASSFD010000102         5409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_788706, whole genome shotgun sequence.
ACCESSION   DASSFD010000102 DASSFD010000000
VERSION     DASSFD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788706"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VFH39_02295"
     CDS             <1..1216
                     /locus_tag="VFH39_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET6924637.1"
                     /translation="GFEKLPKQNVDFGGGLERIAAAKLNDPDVFKISLMKPIVTKLEE
                     ISGKKYDSHTESMRVIADHLRAAAFLAVDGVVPSNKTQGYVMRRLVRRAIRFAFELGV
                     EQNFLEQIVPVIADLYEADYPEVKADRVKVIEVLVKEEKAFRQTLRKGLQQLEKMKYE
                     PGAFEKMGDRGDLPYPRPFTGEDIFLLYDTYGFPAELSVESATMQGLIVDPSWRTEFD
                     EKMAQQRAKSQTAAKGVFKGGLGGQTMQHKKYHTATHLMYQALRDVLGDHVVQHGSNI
                     TEERLRFDFSHPDKMTPEQIQQVESIVNEQISKDLKVSYAEYPTKVAREEMGALGAFG
                     DRYGDTVKVYKMIADGAEKPFSFEICGGPHVDHTGQLAEPDAGTDKPKRFKIVKEESS
                     SAGIRRIKAILA"
     gene            complement(1307..2260)
                     /locus_tag="VFH39_02300"
     CDS             complement(1307..2260)
                     /locus_tag="VFH39_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924638.1"
                     /translation="MNRSGLPNVRYQPTYHFEWEVPADPETFFHAQIAAEKILSVERN
                     CIEQRMVYAGIPCKYAAIPEDFKEAIPDTPIQSLDINFSLTVPFEKGGTRFNFHDTRT
                     PARVLQLKELAPRIMTRMTRPGDYAVLDNYPERAHPSQPIIAREVVNRFAAAQKYGRP
                     EVRPVRLEEEQDLLLYLGKAARNYRQPVIVGEVDYSDTQALHTEKKTRFFLTRAGLRS
                     PGVAYSTWLTGELHDEALGLAEGVRVRTLAVTDGSNPANPNNHLLYQVDAVHAHEPDE
                     LTQLLARTDRRRIARLFKECLRDYLQRPAETDLTWQAALRP"
     gene            2427..2801
                     /locus_tag="VFH39_02305"
     CDS             2427..2801
                     /locus_tag="VFH39_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924639.1"
                     /translation="MEFGFDTSRPPISEAGRTLIEMQRAQAAAAFGIPEEYRPHFIAT
                     GERAPDLAEAQQEPEADTQALAEQAAEVADIVTTVVTGGVGARIHARHETAEALEMPA
                     HHFSEQDLVGAFMTHRIAARSL"
     gene            2869..4224
                     /locus_tag="VFH39_02310"
     CDS             2869..4224
                     /locus_tag="VFH39_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HET6924640.1"
                     /translation="MLDKFKDISRSVSSKAGGALGKAGKAAADKAKNVKTNKGAGDQY
                     IVALDIGTENVKALIGKLNSDDTSIEIIGSGRAHQQLSDMQAGAIADIASVVENCDKA
                     LNQAEQEAGVSVRTAVIGIAGELVKGTTTTVRVARKVPEKALDIAEMEKIIRLVQERA
                     ETQAKKQLAVELGGKEVEVRLVNSALVSIDIDGYPVTNPIGFQGRDIVIQLYTAYAPM
                     IHIGALERTAQELDLDLLAVAAEPFAVARSVIGDNPNQNLSVILMDVGGGTTDIAVVN
                     EGGVQGTKMFGIGGRAYTRSVERDLGVDFEEAERFKLGLQTNLVPRAKAPAVEGALEK
                     TLDVWLSGVELALGEFTKLDHLPSQIFLCGGGSSLSMLKEELSDSVWYKSLPFTRKPS
                     VQYINPTQVAGIADTTGNVKDHTYITAMGLLRVGLDTLQFAGNSDSGSIRDKLDRMLR
                     V"
     gene            4317..>5409
                     /locus_tag="VFH39_02315"
     CDS             4317..>5409
                     /locus_tag="VFH39_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924641.1"
                     /translation="MPASNNKDTIYVDIDDEITTLIEKVKNSDQRIVALVLPKRASVL
                     QSIVNMKLLKRSADQAKKHIVLITSEAGLLPLAGAVGFYVAKNLQTKPEIPGVPGEAA
                     DIVDDTAEDEPIEFNAESASDKPVGELAAAAGAPLAAGKADDAIETLQLDDDTSEETD
                     AAAASAAAGAATAAATHKQKKNRKLAVPNFNRFRVLLVLGALLLILIIVGLYLAAVVL
                     PKGTITISTDTNDVNSHLSLTADTSAQSLDPQNMIVPAQVVQDQKNNSQQVPTTGKKN
                     LGDKATGNVSMSTSDCSGKAPTIPAGTGVSSNGMTYITQQDASLTPKLDGNFNCYYTA
                     SGVPITAQSAGSSYNATLKNAAVADSDYPG"
BASE COUNT         1295 a         1527 c         1600 g          987 t
ORIGIN      
        1 gggcttcgaa aagctgccca agcagaatgt ggacttcggt ggcgggctgg agcgaatcgc
       61 cgctgccaag ctgaacgacc cggacgtgtt caagataagc ctcatgaagc ccatcgtgac
      121 caagctggaa gagatatcgg gcaagaagta cgacagccac accgagagca tgcgcgtgat
      181 tgccgaccac ctgcgggcgg cagccttcct ggcggtcgac ggcgtggtgc cgagcaacaa
      241 gacccagggc tacgtgatgc gccggctggt gcgccgggct atccgcttcg ccttcgagct
      301 gggcgtggaa cagaacttcc tggagcagat cgtgccggtc atcgccgacc tgtacgaagc
      361 cgactacccg gaggtcaaag ccgaccgcgt caaagtcata gaagtcctcg taaaagaaga
      421 aaaagctttc cgccagacac tgcgcaaagg cctgcagcag ctcgaaaaaa tgaaatatga
      481 gccaggtgcc tttgagaaaa tgggcgatcg cggcgatctt ccctatccac ggccctttac
      541 tggggaagat atctttcttc tctatgacac gtatggtttt ccggctgaac tcagcgttga
      601 atcagcaact atgcaggggc taatcgtcga cccatcatgg cgaactgagt tcgacgaaaa
      661 aatggcccag caacgggcaa aatcgcagac ggcggccaag ggcgtgttca agggcgggct
      721 gggcggtcag accatgcagc acaagaagta ccataccgca acgcatctta tgtaccaggc
      781 gctgcgcgac gtgctcggcg accacgttgt tcaacatggt agcaacatca ccgaggagcg
      841 gttgcggttc gacttcagcc acccggacaa aatgacacca gagcagattc aacaggtcga
      901 gagcatcgtg aatgagcaga taagcaagga ccttaaggtc agctacgccg agtatccgac
      961 caaagtcgcc cgcgaagaaa tgggcgcact gggcgcattc ggcgaccgct acggcgatac
     1021 ggtcaaagta tataaaatga ttgccgacgg cgccgagaag ccgttcagct tcgaaatctg
     1081 cggcggcccg cacgtggacc acaccggcca gctggccgaa cccgacgccg gaaccgacaa
     1141 gcccaagcgc ttcaaaatcg tcaaagaaga atcctcctca gctggaatca ggcgaattaa
     1201 ggcaattctg gcttgatcgc gacggttctc tcacgcgtca acagcctaag gttaccggtc
     1261 tctcttagcc agccgcacta gggcaatcct gggatagctg tagttattag gggcgcagcg
     1321 cggcctgcca ggtcaaatcg gtttcggcgg gccgttgcag gtaatcgcgc aggcattctt
     1381 tgaatagccg ggcgatccgg cgacgatcag tccgtgccag tagttgcgta agctcatccg
     1441 gttcgtgcgc atgtaccgcg tcgacctgat acaacagatg attattcggg tttgccggat
     1501 tcgacccgtc cgtcactgct aacgtgcgga cgcggacacc ctcagccaac ccaagcgctt
     1561 catcgtgcag ttccccggtc aaccaggtac tgtaggcgac ccctggtgat ctcaggccgg
     1621 ctcgtgtcag aaaaaaacgg gttttctttt cggtgtgcag cgcttgcgta tcggaataat
     1681 ccacttcgcc gacaatgaca ggctgacggt aattcctggc agccttaccg aggtaaagga
     1741 gtagatcttg ttcttcctcg agccggacag gtctgacttc cgggcggccg tacttttggg
     1801 ctgctgcgaa acggttcaca acttcccggg ctataattgg ttgagaagga tgagcccgct
     1861 ccggataatt atcgagcacc gcgtagtcac ccggccgtgt catgcgggtc atgattctcg
     1921 gcgctaactc cttgagctgc agaacacggg cgggcgtacg ggtatcgtgg aaattgaaac
     1981 gcgtgccgcc tttctcgaaa ggaaccgtaa gcgagaaatt gatatccagc gattgtatcg
     2041 gcgtgtcagg gatcgcttct ttgaagtctt ccggtatcgc ggcatattta cagggtatgc
     2101 cggcatatac catgcgttgc tcaatgcagt tacgctcgac gctaagtatc ttttcggccg
     2161 cgatttgcgc atggaagaag gtctccgggt ccgccggcac ttcccactcg aaatggtatg
     2221 tcggctgata gcggacattg ggcaacccgg aacgattcat ctgagaaact atacaataca
     2281 gcgcaacgga atgccagcaa tgattctatg tattccgcac atgtgacaaa tatcacaaaa
     2341 tcggttgatt tatggaggaa aggggcgtat atttcggctt aagttatgta agcggagccg
     2401 cgaccgttcg atcgcgggca aaaagtatgg aatttggatt cgacacgtca cggccgccta
     2461 taagcgaggc tggtcggaca ttgattgaga tgcagcgagc gcaggccgcc gctgcgttcg
     2521 gcatacccga ggaataccgc ccgcatttca tcgccacagg agaacgagcg cctgacctgg
     2581 ctgaggctca gcaagaacca gaagccgaca cccaggcgct ggctgaacaa gcggccgaag
     2641 tcgcggacat cgtaactaca gtagtgaccg gtggcgtcgg cgcccggatt catgcccgtc
     2701 atgaaaccgc agaagcctta gagatgcccg cccatcactt cagcgagcag gatctggtcg
     2761 gggcgttcat gacccaccgc atcgctgccc gcagtctata aatcacggca gaatcggcag
     2821 cgaaacgcct cgcggggcgg ccggtacgtg ctaaaataga aaaaccatat gcttgataaa
     2881 ttcaaagata tcagccgctc tgttagcagc aaagccggcg gagccttagg gaaggccggc
     2941 aaagcggccg ccgacaaggc taagaatgtc aaaaccaata aaggggccgg cgaccagtac
     3001 atcgtggcgc tggacatcgg cacggagaac gtcaaggcgc tcatcggtaa gctgaacagc
     3061 gacgacacca gtatagagat catcggcagc ggccgagccc accagcagct cagcgacatg
     3121 caggccggcg ccatcgccga catcgccagc gtggtggaga actgcgataa agcgttgaac
     3181 caggccgagc aggaggctgg tgtcagcgtc cgcaccgcgg tcatcggcat tgccggtgaa
     3241 ctggtgaagg gcacgacgac cacggtgcgc gtggcccgca aagtgcccga aaaagcgctc
     3301 gatatcgccg aaatggagaa gataatccgc ttggtgcagg agcgggccga aacccaggcc
     3361 aagaagcaat tggccgtcga gcttggcggc aaagaggtcg aggtgcggct ggtgaacagc
     3421 gcgctggtga gcatcgacat cgacgggtac cctgtgacca atccgatcgg cttccagggt
     3481 cgcgacattg ttattcaact gtacaccgcc tacgcgccga tgatccacat cggcgccttg
     3541 gagcgcaccg cccaggaact ggaccttgac ttgctagccg tagcagccga gccgttcgcc
     3601 gtcgcccgtt ccgtcatcgg tgataatccg aaccagaacc tcagcgtcat cctcatggac
     3661 gtcggtggcg gtacgaccga catagccgtc gtcaatgaag gcggcgtgca aggcaccaag
     3721 atgttcggta tcggcggccg cgcttatacc cgcagcgtcg agcgcgatct gggcgtggac
     3781 ttcgaagaag ccgagcgctt caagctcggc ctgcagacca atctggtgcc ccgggccaaa
     3841 gccccggccg tcgagggtgc gcttgagaag acgttggacg tctggctgag cggtgtcgaa
     3901 ctggcgctgg gcgagttcac caagctggac catctgccga gccagatctt cttgtgcggg
     3961 ggcggctcca gcttgtccat gctcaaggaa gaactgagcg acagtgtctg gtacaagagc
     4021 ctgccgttca cccgtaagcc gagcgtgcag tatataaatc cgacgcaggt cgccggtatt
     4081 gccgacacca ccgggaatgt gaaagaccat acctacatca ccgccatggg cctgctgcgg
     4141 gtcgggctgg acacgctgca gttcgccggc aacagcgaca gcggctccat ccgcgacaaa
     4201 cttgaccgta tgctgagagt gtagccgtaa gaggaatact tgattgaccg ccgaagtggt
     4261 acaatggtgc ttagcatgag cgccttgcgt aacaacagag gtgccaggaa tgaataatgc
     4321 cagccagcaa taacaaagac accatctacg tcgacatcga cgacgaaatc acgacgctga
     4381 tcgagaaggt caagaacagt gaccagcgga tcgtcgccct ggtcctgccg aagcgcgcca
     4441 gcgtgctgca gagtatcgtc aatatgaagc tgctaaagcg cagtgccgac caggccaaga
     4501 agcacatcgt gctcatcacc tcggaagccg ggctgttgcc gctggccgga gccgtcggtt
     4561 tctatgtagc caagaacctg cagacgaagc cagaaattcc gggtgtaccc ggtgaggccg
     4621 ccgacatagt tgacgacaca gcagaagacg agccgattga attcaacgcc gaaagcgcca
     4681 gcgacaagcc ggttggtgag ttggcagctg cagcaggtgc gccactggct gccggcaagg
     4741 ccgatgacgc catagaaacg ctgcagctgg acgatgatac atccgaggag accgatgcgg
     4801 cggcggccag tgccgcggcc ggtgccgcca ccgcggcggc gactcacaag cagaagaaga
     4861 accgcaagct ggcggtgccg aacttcaacc gcttccgggt gctgttggtg ctgggtgcat
     4921 tattactcat tctaatcatc gtcggattgt acctggcggc ggtcgtgctg ccgaagggta
     4981 ccatcaccat cagtaccgac accaacgacg tcaacagcca cctgagcctg actgccgata
     5041 cgtccgcgca gtcactcgac ccgcagaaca tgatcgtgcc ggcgcaggtc gtccaggatc
     5101 agaagaacaa ttcgcagcag gtaccgacga ccggcaagaa gaaccttggc gataaggcta
     5161 ccggtaatgt gtctatgtcg acttctgact gttctggcaa ggcgcctact atcccggccg
     5221 gtacgggcgt ttcttctaat ggaatgacct acataaccca gcaggacgca agtcttacac
     5281 cgaaactcga cggcaacttt aactgctact atacggcaag tggcgtaccg attacggccc
     5341 aaagtgctgg cagtagctat aatgcgacat taaaaaatgc tgccgtagca gattcagatt
     5401 atccagggg
//
LOCUS       DASSFD010000103        10807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_191555, whole genome shotgun sequence.
ACCESSION   DASSFD010000103 DASSFD010000000
VERSION     DASSFD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191555"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..622)
                     /locus_tag="VFH39_02320"
     CDS             complement(74..622)
                     /locus_tag="VFH39_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6924642.1"
                     /translation="MLARRQYRKKRFKKFLPDFIADSVEAIDFDRLAERGVKACLIDL
                     DGTVVARAQYEVTGPVQRALKNASQRIYIATNRPKSRDLKDLRIDLNADGVVHPKGKL
                     GKPFKRYYHAALHDLGLQEGQVVMIGDRYIQDIFGANNAGLYTVLIRNKVGPAHDWMD
                     SLLSRAERLLTRLIKPAYDELR"
     gene            complement(677..753)
                     /locus_tag="VFH39_02325"
     tRNA            complement(677..753)
                     /locus_tag="VFH39_02325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Pro,seq:tgg)
     gene            844..1002
                     /locus_tag="VFH39_02330"
     CDS             844..1002
                     /locus_tag="VFH39_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924643.1"
                     /translation="MKYASIFATILFIWIAVIIMAATTTDAKQIYGLYLASLISTLAL
                     FVIGFARK"
     gene            989..2614
                     /locus_tag="VFH39_02335"
     CDS             989..2614
                     /locus_tag="VFH39_02335"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET6924644.1"
                     /translation="MPESKHRMARRTKPVTDTSLRALKRQRTVRLARLLSRAFYLRRR
                     GRVSEMYNLICDEMVSLGGVYVKFLQGVLLNGKVMRQWVNPDRLKIFENLETEPLDII
                     GILHHELPSERLKQITQIQPQPFAAGSFGQVYLGVHANGKHIIIKVLRPQVRELLRHD
                     LRLLGIFAKRFTAGEYKNISVKMDEAIRDFRNVTLRETDYVSEAEFAHELFVAYKGNP
                     KMVIPETYMDLCTKHVIVQEYLGGVSAVDLLKMRENGIDVVKYVADTLGSDLDQQLIT
                     LGVESLRGAFTLPRVQGDPHPGNIRFLPDNKVGMIDFGISAKSPENKAAFFGVISEWS
                     RMYEEKSNVGRLFEQFMRFFMSDLYRALRKLSSLRSTSQNSGSNSDGSQFNFAKELGR
                     MMQEILHNTLGTYDLQELLEDGRMLYIFNNMVNKGNRFGLVVKLEASEILRASQTYMT
                     LTEALGRRRVVMPQVLANVVQEVEAQYPQLRTETEDNISMTQALEIINLWLERVAMRD
                     PALFDNLMRRIKLHTTKTASTIKVEPPTEPSNA"
     gene            2607..2795
                     /locus_tag="VFH39_02340"
     CDS             2607..2795
                     /locus_tag="VFH39_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924645.1"
                     /translation="MLKEFLLFIKYPYTAGVIATIWLATAILMAINRDLPAIKMVIIN
                     MFASVFLALIGFGGKTES"
     gene            complement(2792..3589)
                     /locus_tag="VFH39_02345"
     CDS             complement(2792..3589)
                     /locus_tag="VFH39_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET6924646.1"
                     /translation="MRMLYIGDVMGPVGIAVVEAVLPGLRKEEGLDTVIAQAENVTDG
                     KGLGIADYKRLQAAGVDGFTGGNWTTALPETATLLNDPNVPVTGPANMPDCPGPGYKY
                     IVTPKGRLLIISLLGKIVGRDADKPTDNPLIKIDEILESQKDEPRVATVVNFHGDYSS
                     EKVVFGHYLDGRVSVVVGDHWHVPTADAMVLPAGTAYRTDVGMCGSLDSSLGVRYASV
                     IPRWREGQQTRNVLETEGRKQFNALLVEIDEATGKAMTAEAIRQIFD"
     gene            complement(3622..4242)
                     /locus_tag="VFH39_02350"
     CDS             complement(3622..4242)
                     /locus_tag="VFH39_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924647.1"
                     /translation="MAGNLENLQELFPGIDPAELGALQQIEDNFFADEVRSRLNSLKF
                     VDGICNLYSEPASDGYSEVLMANIVLRVFGKDRSILWAVRSDDTTDDEMHPAKSITNA
                     YIDLAGEPGRRAAVFEPVLLTQRGDGSWRHTTDSPPFPDYDRGPRRLTVRHGHRYNVV
                     NIRNMLEYHFSSAAEYEFLGKIAHLTEGSKEPIDGAPTYELSTNPD"
     gene            complement(4296..4904)
                     /locus_tag="VFH39_02355"
     CDS             complement(4296..4904)
                     /locus_tag="VFH39_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924648.1"
                     /translation="MSHVDALDRLIGEYNSRPEEAARLERSIAFGALQDMAERLFGSP
                     GPAALSTTTFIDGAEREYLLIRHRARKGRPLALRLLMNEGDLAMEDMTKYVVTGTELV
                     ALPTRGLIAKRVCRMLEREDGEFDIGNEGFLLNPPDDTGKVVVRHGLGYRPGFPTDSP
                     STEQAALTARGQLAREMTSLLSGMVHEEALGVDYLFMPQLHA"
     gene            complement(4909..6417)
                     /gene="rny"
                     /locus_tag="VFH39_02360"
     CDS             complement(4909..6417)
                     /gene="rny"
                     /locus_tag="VFH39_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET6924649.1"
                     /translation="MLEALLAIVGLAVGFGANAVVTRQRMGSAQAQADKELAKAKKEA
                     EKAKAEAREEALRIAEEGRKEEQSRRRELKDLENRLIQREESLDKKLDDLDKRTEKLR
                     KAEDEVEALKNEIRDIRGKQQEKLEKIAKLSKAEAAEKLMQMTERDIRGDLTGLVSKL
                     QNEAREDAEEQAGLIIATAMERMASEVTAERTITSVKLEDEEMKGRIIGKEGRNIQSL
                     QRATGVDIMVDDTPGYIVLSSFDPVRREVARQALEMLMKDGRIHPGRIEEVVEKAQKN
                     VEKDVIRAGEDAAREVGVIGIPKEILHLLGELKYRTSYGQNVLKHSTEMAHLAGMIAE
                     ELGANIKTAKYAALVHDLGKALTHKMEGKHHHISGEVLRKYGAPEEVALAAEQHHDDV
                     EATSVEALIVRVCDAISASRPGARNISAENFAERMKELENVANSFNGIEKSYAISAGR
                     EVRVFVKPQAIDDLSAIKLARDIATKIESSMQYPGTIKVNVIRETRAIEFAK"
     gene            complement(6602..6994)
                     /gene="tsaB"
                     /locus_tag="VFH39_02365"
     CDS             complement(6602..6994)
                     /gene="tsaB"
                     /locus_tag="VFH39_02365"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6924650.1"
                     /translation="MLIITIRTDKPEAEIGLYENTALLVADRWLAHRQLAETLHGRLR
                     QLLETQGKQWRDVQGIAVFQGPGSFTGLRIGISVANALAYSLQVPVAGAEDPDWTDAA
                     IDSLVRGEAGKLVVPEYGALPHITPQKK"
     gene            complement(6996..7388)
                     /gene="tsaE"
                     /locus_tag="VFH39_02370"
     CDS             complement(6996..7388)
                     /gene="tsaE"
                     /locus_tag="VFH39_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6924651.1"
                     /translation="MRCKGGEVIELISDLGGGKTTFVRGLAAGLGSPDSVASPSFTLS
                     REYKAADKTLYHFDFYRLSDPGLVANELAEVVGNPQAVVAVEWADIVEDVLPAAKLTI
                     RLTNTGESERKLEFSAPETLKYLLPENT"
     gene            complement(7369..8793)
                     /locus_tag="VFH39_02375"
     CDS             complement(7369..8793)
                     /locus_tag="VFH39_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6924652.1"
                     /translation="MNRLVRRITEWLSLVFIMLSSPILVSATGQPPALKAPDTLALAE
                     LKITGDEYVRLQNNGSSDIDDLSSYWLYDFNNVNPLAGGVSSSQQQLPAVGLKQGQSL
                     LLSGSPRNTCGATVAGKLNVSLTDGGGFLQLVRLSQDASGVKQTGGDMVSWSNGANGI
                     IQNVPSSSKDPQGTNMYYRYQMPDGYGWQLAAMDASDSCSLHVAASGTSTPVSMGSLS
                     GVTAEPPATIISLADQADSATDTPTLPLSDIGLMSPVINELLPNPAGTGNDSTDEYIE
                     LYNPNPKSFDLSGFTLETGLTTKHKYIFPADTLLAAHSFTAFYAADTHLTLSNSGSQA
                     ALLDPFGTVINQSDPFGTAKDGVAWALAKGEWYFTSDPTPNAANVIKQASTTKTKSSK
                     AKSAAAVKGAATSSAGVGGTASGNDTQVAQLTPIHPWTLAIVIALALAYGLYEYRFDL
                     ANRFYQFRKHRAHRARTRYAVQGR"
     gene            8888..9403
                     /locus_tag="VFH39_02380"
     CDS             8888..9403
                     /locus_tag="VFH39_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924653.1"
                     /translation="MLQLSKTLLNQPVMSLRTGSPVATAVAPVINPDNLKVEGFYCTD
                     QFSGKQLILLYQDIRDVIPQGFVVDDHDSLSDPEELIRLRDVLNLRFDVIGKPVQTIS
                     KEKVGKVSDYAVETTTMYIQKLYVSQSLLKSFTGGSLSIDRTQVHEITEKRIIINDLL
                     QGVPAAMPAGV"
     gene            complement(9408..10019)
                     /locus_tag="VFH39_02385"
     CDS             complement(9408..10019)
                     /locus_tag="VFH39_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET6924654.1"
                     /translation="MIVTAIKQQVRQKGRYSVFVEGKYSFSLSDSALLESKLVIGQEL
                     TGEEVKRFKQLSGDDKLQSMALRYAAMRPRSTWEMEQYLKRKKAAPLLHQQILSKLSN
                     LNLLDDRSFAESWVASRRLLKPVSYRRLQAELRAKHVPDEVITEVLAGDETDERTTLR
                     DLVARKRKQSKYQDQTKLMQYLARQGFSYDDIKSVLSEETEEA"
     gene            complement(10016..10363)
                     /locus_tag="VFH39_02390"
     CDS             complement(10016..10363)
                     /locus_tag="VFH39_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924655.1"
                     /translation="MSPNLTVIDTAPVRFTFNAEVDLRATPYVIPPSRQAFLAGMALE
                     AYVTRGRETGLLIDMDEVITSLELDEEPRRRAQAFFWKGLRDGIRHGTRQKTVWLNVP
                     ATEGVIMDKSTAA"
     gene            complement(10388..>10807)
                     /locus_tag="VFH39_02395"
     CDS             complement(10388..>10807)
                     /locus_tag="VFH39_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924656.1"
                     /translation="VHRPALALPLALLSHFVLDALPHYGYGYIPHHERDQQRNFLRKQ
                     TADTYFALLLFWVVPYVLRHQQAPIVTMLCMLAGFLPDVIWPYQYVMAQRRGGYKQPD
                     WYARFHKAIQWCERPWGLWVELGWLAASLTAVVVIAN"
BASE COUNT         2157 a         3270 c         2973 g         2407 t
ORIGIN      
        1 taccggttgg cgaccttctg gctgccgata cccttcagcc tgctggccta ccgctacatt
       61 acccgccgca agcttatcta agttcgtcgt aggccggttt gatcagccgg gtcagcagcc
      121 gctccgcgcg cgacagcaga ctgtccatcc agtcgtgggc cggcccaacc ttattgcgga
      181 tgaggaccgt gtacagtccg gcgttgttgg cgccgaagat gtcttgaatg tagcggtcgc
      241 cgatcatcac cacctgtccc tcctgcaggc caaggtcatg caatgccgca tggtagtaac
      301 gtttgaacgg cttgccgagc ttgcccttgg gatgcaccac gccatccgca ttcaaatcga
      361 tccgtagatc cttcaggtca cggctctttg gccggttggt ggcaatataa atccgctgcg
      421 aagcgttctt aagtgccctt tggaccggac cggttacttc gtattgggcc cgggcgacca
      481 ccgtaccgtc caggtctatc aggcacgcct tcaccccccg ctctgccagg cggtcgaaat
      541 cgatcgcctc gacagagtca gcaatgaagt ccggcaggaa cttcttaaat cgttttttac
      601 ggtattggcg tcgggctagc atgccctgca gtataacaaa agaggcctgc gctgcaggcc
      661 tcaataattc acgatgtggt cggggataga ggactcgaac ctccgacctc cgcgtcccaa
      721 acgcggcgcg ctagccaact gcgccaatcc ccgtaaggct taggtgatta taccgaataa
      781 caggggaaaa ggccagtcgc cagcccttcg acgcttaccg ggtgtttatg atataataac
      841 tctatgaaat acgcatcgat ctttgcgacc atactgttca tatggatcgc ggtcatcatc
      901 atggctgcca ccacgaccga tgccaagcag atttacgggc tgtacctcgc cagcctcatc
      961 agcaccctgg ccctattcgt aatcggcttt gccagaaagt aagcaccgga tggctcgccg
     1021 caccaaaccg gtcacggaca catctttgcg tgccctcaag cgccagcgca cggtgcgact
     1081 ggcacgcctt ttaagccggg ccttctacct gcgtcgccgc ggccgggtca gcgagatgta
     1141 caacctcatc tgcgacgaaa tggtctcatt gggcggtgtc tacgtgaagt tcctgcaagg
     1201 agtgctactg aacggcaagg tgatgcgcca gtgggtcaac cccgaccggc tgaagatttt
     1261 tgaaaacctg gagaccgaac cgctcgacat catcggcatt ctgcaccacg agttgccttc
     1321 agaaaggctg aagcagataa ctcagatcca gccgcagccg tttgcggccg gtagcttcgg
     1381 ccaggtgtac ctgggcgtcc acgctaatgg caaacacatc atcatcaagg tattgcgtcc
     1441 gcaggtgcga gagctgctgc ggcacgacct gcggctgctg ggcatcttcg ccaagcgctt
     1501 cacggccggc gagtacaaga acatcagcgt gaagatggat gaagccatcc gggacttccg
     1561 caacgtgaca ttgcgcgaga ccgactacgt gagcgaggcg gaattcgccc atgagctctt
     1621 cgtggcctac aagggcaacc ctaaaatggt catccccgaa acctacatgg acctgtgcac
     1681 caagcacgtc atcgtgcagg aatacctcgg aggcgtctcc gccgtcgacc tgctgaagat
     1741 gcgcgaaaac ggtatcgacg tggtcaagta cgtcgccgac acgcttggct cggatctcga
     1801 tcagcaactc atcacgttgg gagtggagag tctgcgcggc gccttcacac tgccccgtgt
     1861 ccagggcgac ccgcatccgg gcaatatccg gttcctgccc gataacaaag tgggcatgat
     1921 tgatttcggc atctccgcca agagccccga gaacaaagcc gcattcttcg gagtcatcag
     1981 cgagtggagc cggatgtacg aagaaaagtc taacgtcggc cggttgttcg aacagttcat
     2041 gcgtttcttc atgagcgatc tctaccgcgc cttgcgcaag ctcagcagcc tgcgcagcac
     2101 cagccagaat tcgggcagca acagcgacgg cagccagttc aatttcgcta aggagctcgg
     2161 tcgcatgatg caggaaatcc tgcacaacac gctcggtaca tacgatctgc aggagttatt
     2221 ggaagacggt cggatgttat acatcttcaa taacatggtg aacaaaggta accgcttcgg
     2281 cctggtggtc aagctggagg cttccgaaat tctacgcgcc tcccagacgt acatgaccct
     2341 tacggaagcg ctcggacggc gccgtgtcgt gatgccccag gtgctggcta acgttgtcca
     2401 ggaggttgag gcccagtatc cgcagctccg cacggaaacc gaagacaaca tttccatgac
     2461 ccaggcgctg gagatcatca acttatggtt ggaacgggtg gccatgcgcg atcccgccct
     2521 gtttgataat ctgatgcgac gtattaaact acatactact aagacggcat ccaccataaa
     2581 ggtcgagccg ccgacggagc caagcaatgc ttaaagaatt cctgcttttc atcaaatacc
     2641 cctacaccgc cggagttatc gccaccatct ggctggcaac ggccatcctc atggctatca
     2701 accgcgattt gccggccatc aaaatggtca tcatcaacat gttcgccagc gtgtttctgg
     2761 cgctcatcgg tttcggcggc aagaccgaaa gctaatcaaa tatctgccgg atagcttccg
     2821 cagtcatggc cttaccggta gcttcgtcaa tctcaacgag cagggcgttg aactgtttgc
     2881 gaccttcggt ctcgagcacg ttgcgggtct gctgtccttc gcgccagcgc ggaatgacac
     2941 tggcatatcg cacacctaga ctgctgtcga gcgagccgca catgccaacg tcggtccggt
     3001 aggctgtgcc cgccggcagc accatggcat ccgccgtagg cacgtgccaa tggtcgccaa
     3061 cgaccactga cacccgtccg tccaggtagt gcccgaagac gaccttctcg ctgctgtagt
     3121 caccatggaa attcactacc gtagctacgc gcggttcgtc cttttgcgat tccaggatct
     3181 cgtcgatctt gatgagcgga ttatccgtcg gcttatcggc gtcgcgcccg actatcttgc
     3241 ctagcagact gatgatgagc agccgaccct tcggcgtaac gatatattta tagccaggac
     3301 cgggacaatc cggcatgtta gccggcccgg tcaccggcac gttcggatca ttgagcaagg
     3361 ttgcggtctc gggcagcgcc gtcgtccagt taccgcccgt aaagccgtca actcctgccg
     3421 cctgcagacg tttataatcg gcaatcccca ggcctttgcc atccgtcacg ttctcggcct
     3481 gcgcaattac cgtatcgagg ccctcctctt tcctcaagcc aggcaaaacc gcctcaacga
     3541 cagcaatacc caccggtccc atgacatcac cgatatagag cattctcatg cacctattgt
     3601 ataagatgtt tcgcttgagc ctcaatccgg gttggtagaa agttcataag taggcgcacc
     3661 atctatcggt tccttggagc cttcagtcag atgcgcgatt ttacccagga actcatattc
     3721 ggcagcagaa ctgaaatggt actccaacat attcctaatg ttcacaacgt tgtaccgatg
     3781 cccgtggcgc acagttaaac ggcgtggtcc gcggtcgtaa tccgggaagg gcgggctgtc
     3841 ggtcgtatgt cgccacgaac catcgccccg ctgcgtcaac agaacgggct cgaaaacagc
     3901 cgcccgccta cccggttccc ccgccaggtc gatgtaggca ttggtgatgc ttttggcagg
     3961 gtgcatctcg tcatcggtag tgtcgtcgga tcggaccgcc cacaagatgc ttcggtcttt
     4021 gccgaagacg cgcagcacga tgttggccat caaaacctcg gagtagccat cgctggctgg
     4081 ctcgctatat aaattacata tcccatccac aaacttaagc gagttcagcc ggcttcgtac
     4141 ttcgtcggca aagaagttgt cctcgatttg ttgcagcgca ccaagttctg ccgggtcgat
     4201 tcccggaaac agttcttgta aattctccag attgccagcc atgttatgcc ttcgcttgaa
     4261 cctgagtgta gacccgtttc gctggaagtc aagcgctagg catgaagctg tggcatgaac
     4321 aaatagtcca cgcccaaggc ttcctcgtgc accatacccg aaagcaaact cgtcatctcg
     4381 cgcgcaagct ggcctcttgc cgtcaatgcg gcctgctcgg tggagggtga gtccgtcgga
     4441 aatcccggac gatagcccag accgtgacgg acgacaacct tgcccgtatc gtcaggaggg
     4501 ttcagcaaaa acccctcatt accgatgtcg aattcgccgt cttcacgttc gagcatccgg
     4561 catactcgct tagcaatcag tccccgcgtc ggcagcgcca ccaactctgt ccctgttacg
     4621 acgtacttgg tcatgtcttc catcgccaag tccccctcgt tcatcaacaa tctgagcgcc
     4681 agcggccgtc ccttccgggc acgatgccgt atcagcaagt attcgcgctc ggcgccatct
     4741 ataaaagtgg tcgtcgacaa cgctgctggc cccggtgacc cgaacagacg ttcagccata
     4801 tcctgcaagg ctccaaaagc tatgctgcgt tccaggcgcg ccgcttcctc cggcctgctg
     4861 ttatattcgc ctatcaggcg atcgagtgca tcgacgtggc tcatctgatt acttggcgaa
     4921 ttcgatggcg cgggtttcgc ggatgacgtt caccttgatg gtgcccggat actgcatgct
     4981 ggattcaatc ttggtcgcga tgtcgcgggc cagcttgatg gcgctcaggt catcgatggc
     5041 ttgcggcttc acgaacaccc gtacctcacg gccggcgctg atggcgtagc tcttctcgat
     5101 gccgttgaaa ctgttggcga cgttctccag ctctttcatc cgctcagcga agttctcggc
     5161 ggaaatgttg cgggctcccg gccgtgaagc gctgatggcg tcacaaacgc gcacgatcaa
     5221 cgcttcgaca ctggtcgcct cgacgtcgtc gtggtgctgc tcggcagcca gggcgacttc
     5281 ttcgggcgca ccgtatttac gcaggacctc accggaaatg tggtgatgct tgccttccat
     5341 cttgtgcgtc agagccttgc ccaggtcgtg gaccagtgca gcgtacttgg cggtcttgat
     5401 gttagcgccg agttcttcgg cgatcatgcc ggccaggtgc gccatctcgg tgctgtgctt
     5461 gagcacgttc tggccgtagc tggtgcggta cttcagctca ccgagcaagt gcaggatttc
     5521 cttcggaatg ccgatgacgc cgacttcacg ggcggcgtct tcaccggcac ggatcacgtc
     5581 cttttcgaca ttcttttggg ccttctcgac gacttcctcg atacgtccag ggtggatacg
     5641 gccgtccttc atgagcatct caagcgcctg gcgggcgact tcgcggcgta ccgggtcaaa
     5701 gctgcttagc acgatgtagc ccggcgtgtc gtcgaccatg atgtcgacac cggtggcgcg
     5761 ctgcagcgac tggatgttgc ggccttcctt accgatgata cggcccttca tttcctcgtc
     5821 ttccagtttg acactggtga tggtgcgctc cgccgtcact tcgctggcca tgcgctccat
     5881 ggcggtggca atgatcagcc cggcctgttc ctcggcgtct tcacgggcct cgttctgcag
     5941 cttggacacc aggccggtca gatcgccgcg gatgtcgcgc tcggtcatct gcatcagctt
     6001 ctcggccgct tccgccttgc tcagtttggc gatcttttcg agcttttcct gctgtttgcc
     6061 gcggatgtcg cggatttcgt tcttcagcgc ctcgacttca tcctcggctt tgcgcagctt
     6121 ttcggtacgt ttgtccaggt catccaactt cttgtccaac gactcttcgc gctgaatcag
     6181 gcggttctcc agatccttca gctcacggcg acgggactgt tcctctttgc ggccttcttc
     6241 ggcgatacgc agggcttctt cgcgcgcctc ggctttggcc ttttcggcct cttttttagc
     6301 tttggcgagt tctttgtcgg cctgggcctg ggcggaaccc atgcgctggc gagtcaccac
     6361 cgcattggca ccaaagccca ccgcaagccc aactatagcg agcaatgctt cgagcataat
     6421 ttgtcctttc tgtctgcaga tatcgggctg ctcggcatcg ctatcacttc tctagccggc
     6481 ggtcgtcatc tgataatcga attaattaaa actggatcgg tcaagagccg gttgtctctc
     6541 aaactctcgt tcctgtcctc aatataccgc gcgacataat ccgccgtcaa tcaagtgagg
     6601 attacttttt ctgcggcgtt atatgcggca gggcaccgta ttccggtacc accaatttgc
     6661 cggcctcgcc acgcaccagg ctatcgatgg ccgcatcggt ccagtcaggg tcttcggcgc
     6721 cggcgaccgg gacctgcagt gagtaggcca gcgcgttggc cacgctgatg ccgatacgaa
     6781 gccccgtgaa actgccgggc ccttggaaca ccgcgatccc ttgcacatcc cgccactgtt
     6841 tgccttgggt ctcgagcagc tgccgtagcc ggccgtggag tgtctcggcc agctggcggt
     6901 gggccagcca gcggtcagca accagcaggg ccgtattttc atacagacct atctcggcct
     6961 ccggcttatc ggtacggatg gtgatgatta acatgctaag tattctccgg taagaggtat
     7021 ttgagtgttt cgggcgcgct gaattcgagt ttccgctcag actcgccggt gttcgtcaaa
     7081 cggatggtca gcttggcggc aggcaagaca tcctccacga tatcggccca ctccacagcc
     7141 acgaccgcct gcggattgcc aacgacttcg gccagctcgt ttgccaccag ccccgggtcg
     7201 ctcaagcggt agaagtcgaa gtggtacagc gtcttgtcgg cggctttata ttcgcgcgac
     7261 agggtaaagc tgggacttgc cacgctgtcc gggctgccga gccctgctgc cagaccgcgt
     7321 acgaacgtcg tcttgccgcc gcccaggtcg ctgataagtt cgatgacctc accgcccttg
     7381 caccgcatac cgagttcgcg cccgatgtgc tcggtgtttg cggaactgat agaaacggtt
     7441 tgccaggtca aaacggtact catacaaccc atatgctagc gcgagagcga tgacaatcgc
     7501 aagcgtccac ggatgaatcg gcgtcagctg ggccacctgc gtgtcgttcc cggacgccgt
     7561 gccgccaacg ccggccgagg aggtcgccgc ccctttgaca gcagccgccg acttggcctt
     7621 ggatgatttc gtcttggtag tggaagcctg cttaatgaca ttggccgcat tgggtgtcgg
     7681 gtcgctcgtg aagtaccatt cacctttggc gagcgcccaa gcaacgccat ctttggcggt
     7741 gccgaacggg tcagactgat tgatgaccgt gccgaacggg tcgagcagtg ccgcttggga
     7801 accgctgttg ctaagcgtca gatgcgtatc ggcggcataa aaagcggtaa agctgtgcgc
     7861 cgccagcaag gtgtcggctg ggaagatgta tttgtgcttg gtcgtcaggc ccgtctcgag
     7921 cgtaaagccg ctcaggtcga agcttttcgg gttcggattg tacagctcga tgtactcatc
     7981 cgtcgaatca ttaccggtgc cggccgggtt gggcagcagc tcgtttatga ccgggctcat
     8041 cagcccgata tcgctcaggg gaagcgttgg tgtgtcagtt gcactgtccg cctggtccgc
     8101 cagacttatg atggtcgccg gcggttccgc cgtaacaccg ctcagactgc ccatcgacac
     8161 cggcgtgctc gtgccactgg cggctacgtg cagactgcag ctatcggacg catccatggc
     8221 tgccagctgc cagccgtaac catccggcat ctggtagcgg taatacatgt ttgtgccttg
     8281 cggatccttg ctgctggaag gaacgttctg aatgatgcca ttggcgccgt tgctccacga
     8341 caccatgtcg ccgccggtct gtttgacgcc gcttgcgtcc tggctcagac gtaccaactg
     8401 caggaagccg ccgccatcgg tcaggctgac gttaagcttg ccggccaccg tcgccccgca
     8461 ggtgttgcgc ggcgaaccgc tcagcagcaa tgattggccc tgcttcaggc ctacggccgg
     8521 tagttgctgc tggctactgc tgaccccgcc ggccagcgga ttgacattat tgaagtcata
     8581 cagccagtag ctgctcaggt cgtcgatgtc gctgctgccg ttgttttgca gtcgcacgta
     8641 ttcgtcaccg gtgattttca gttcggcaag agcgagcgtg tcaggcgcct tgagggccgg
     8701 gggctggccg gtcgcagaca cgagaatcgg tgagctcagc atgatgaaaa ctaagcttaa
     8761 ccactctgtt atccgccgta ccagccggtt catgcccgcg gattatggcc aagcgcagcg
     8821 gcatttgcaa gcagcagcgt tataaattgt atttaaggag cgtttcgcga ggcgctatac
     8881 taaagacatg cttcagctca gcaagacatt gctcaaccag cccgtcatga gcttacgtac
     8941 cggcagcccg gtggcgaccg ccgttgcgcc ggtcatcaac cccgacaatt tgaaggtgga
     9001 aggtttttac tgcaccgatc agttcagcgg caagcagctg atactgctct accaagacat
     9061 ccgggatgtc ataccgcaag gcttcgtcgt agacgaccac gacagcctga gcgatcccga
     9121 ggagctcatc cgcctgcgcg acgtgctgaa cctccgtttt gatgtcatcg gcaaaccggt
     9181 acagaccatc agcaaagaaa aggtcggcaa agtgagcgat tacgccgtag agacgaccac
     9241 catgtacatt cagaagttat acgtcagcca gtcgctgctc aagagcttca ccggcggcag
     9301 tttaagtatc gaccgtactc aggtccatga gataaccgaa aaacgcatca tcatcaacga
     9361 cttgttgcag ggtgtacccg cggccatgcc ggccggcgtc tagcagacta cgcttcctct
     9421 gtttcttcag aaagaacgct tttgatatcg tcgtagctga aaccttgacg cgccaggtac
     9481 tgcataagtt tggtctgatc ctggtacttg gattgtttac gcttgcgggc caccagatca
     9541 cgcagggtag tgcgttcatc ggtctcatcg cctgccagta cctctgttat aacctcgtcc
     9601 ggcacgtgct tggcacgcag ctcggcttgc agacgccggt aggagaccgg cttgagcagg
     9661 cgacggctgg ccacccagct ttcggcaaat gaacggtcgt cgagaagatt caaattactt
     9721 aacttgctaa gtatttgctg atggagcagg ggagcggctt ttttgcgctt caaatactgc
     9781 tccatttccc acgtggaacg aggacgcatc gccgcatagc ggagggccat ggattgcagt
     9841 ttgtcatcgc cggatagctg tttgaaccgc ttcacctcct cccctgtcag ctcctgcccg
     9901 atgaccagtt tactctcgag caaagcgctg tcgctgagcg aaaacgagta cttcccttcc
     9961 acgaaaacgg agtatctccc cttctgccgc acctgctgtt ttatcgccgt gactatcatg
    10021 ccgccgtgct cttatccatg attactcctt cggtcgccgg cacgttcagc cagacggttt
    10081 tctgccgtgt accgtgtctt atgccgtcgc gcagcccttt ccagaaaaag gcctgcgccc
    10141 gtcgccgcgg ctcttcgtct aactctaagc tggtaataac ctcatccata tctataagta
    10201 atcctgtttc ccgcccccgt gtcacatagg cttccagtgc catgccggcc agaaaagcct
    10261 gcctgctggg cggtattaca taaggggtag cacgcaggtc gacctccgcg ttgaaggtaa
    10321 atcgtaccgg cgcagtatcg ataactgtta aattagggct catgccccta gcgtgccatc
    10381 ttatgcacta attggcaatg actacgacgg ctgtcaacga tgctgctagc cagcccagtt
    10441 cgacccacaa gccccacgga cgctcgcacc actggatagc cttgtggaaa cgggcgtacc
    10501 agtcgggctg cttgtagccg ccgcggcgct gcgccatgac gtattggtag ggccagataa
    10561 cgtccggcaa aaacccggcc agcatgcaca gcatggtaac gatgggcgcc tgctgatggc
    10621 gcagcacata cggcaccacc cagaacagca ggagtgcgaa gtacgtatcc gcagtctgct
    10681 tacgcaagaa gttgcgctgc tggtcgcgtt cgtgatgcgg gatgtagccg tagccgtaat
    10741 gcggcagcgc atcaagcacg aagtgcgaca gcagcgccag cggcaaggcc aacgccggcc
    10801 ggtgcac
//
LOCUS       DASSFD010000104         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_775692, whole genome shotgun sequence.
ACCESSION   DASSFD010000104 DASSFD010000000
VERSION     DASSFD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775692"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFH39_02400"
     CDS             complement(<1..207)
                     /locus_tag="VFH39_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924657.1"
                     /translation="MAAAKKSAPKASVKKVHDIAKPGTTPAGATSRPIIVNNRPLLQD
                     PMVVAAAPGAGAAPELAPAEPAPAA"
     gene            complement(212..991)
                     /gene="tpiA"
                     /locus_tag="VFH39_02405"
     CDS             complement(212..991)
                     /gene="tpiA"
                     /locus_tag="VFH39_02405"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET6924658.1"
                     /translation="MKKTLVVGNWKMHLNVHEASLLVHRLHQAIPVHRSVEVVLAPSM
                     LTLQPLSLEIDSRKFKLAAQDAYFKDEGPYTGEVSFNMLRDLVRYAIIGHSARRIYFG
                     ETLETVRDKVEAAVRNGITPILCIGETKPERLNGETRRVIHDQLTTALANITSEDIED
                     IVIAYEPIWAISTFDGEIAKPDDMQKCLDFIRYEVKELYGEAAAQAVRVLYGGSVDAH
                     DVRAYLELDGCDGVLVGAASLNYKQFPAIVDTAFKVDEAGR"
     gene            complement(1138..>2103)
                     /gene="pgk"
                     /locus_tag="VFH39_02410"
     CDS             complement(1138..>2103)
                     /gene="pgk"
                     /locus_tag="VFH39_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6924659.1"
                     /translation="LKAGQVLLLENLRFHPEEEQNDDNFAQLLAGLADVFVQDGFGVV
                     HRAHASTAAITRHLPSVAGLLLEKEVDTITNVMEKPERPLVAIIGGAKIADKIDLLHR
                     FIAIADFVAVGGAMANTFLAAEGIDVAHSLYDKADLPVARDIMHKARAEARKRRFIFA
                     IPRDGVVASKMDKTASTRIVDWSAHVIADIENYPHRVPARASHVLWQEMILDIGPFSG
                     SFIAGGIQLANTVVWNGTMGVTETKGLQGPVGPYAHGTELVVDALLGQFGHKPFTVVG
                     GGDTVGYLEERKLVGHFGHVSTGGGASLELMAGRKLPGVEALQDK"
BASE COUNT          363 a          650 c          611 g          479 t
ORIGIN      
        1 cgctgcaggg gcgggttctg ccggtgcaag ttccggtgcg gcgccggcgc caggtgctgc
       61 cgccaccacc atcggatcct gcagcagtgg gcggttattc acaatgatcg gccgcgacgt
      121 ggcgccagcc ggcgtagtgc cgggtttggc gatatcgtgc acctttttga cgcttgcctt
      181 cggcgcggat ttcttggcgg ccgccatatg cttaccggcc cgcctcgtct actttgaagg
      241 ccgtgtccac gatggccggg aactgcttgt agttcaagct cgccgccccg accagcacgc
      301 catcgcagcc gtccagctcc agataggcgc gtacgtcgtg ggcatcgaca ctgccgccgt
      361 acagcacacg caccgcttgg gccgcagctt ctccgtacag ctcttttact tcatagcgaa
      421 tgaaatccag gcatttttgc atgtcgtcgg gcttggcaat ctcgccgtcg aaggtactga
      481 tggcccatat cggctcgtac gcgatgacga tgtcttcgat gtcttcgctg gttatatttg
      541 ccagcgccgt ggtcagctgg tcatggatga cgcgccgcgt ttcgccgttc aggcgctccg
      601 gtttggtctc gccgatacac aggattggcg tgatgccgtt acgcaccgcc gcctccacct
      661 tgtcgcggac cgtttcaagc gtctcaccga agtaaattcg ccgggccgaa tggccgataa
      721 tggcatacct gaccaggtcg cgcagcatat tgaacgagac ctcgccggtg tacgggcctt
      781 catccttgaa ataggcgtcc tgggcagcca gcttaaactt acggctgtcg atttccagac
      841 tcagcggctg cagggtcagc atgctgggcg ccagcaccac ttcgacgctg cggtgtaccg
      901 gaatggcctg gtgcagacgg tgcacgagca ggctggcctc atgcacgttg aggtgcattt
      961 tccagttgcc caccacgaga gtctttttca tgaaatactt atggttatcc ttaggctcat
     1021 tttaccacag cagcctatcc gcctaaattt ccaattatcg attttgcaat tttcaatcag
     1081 ggtatctatt ttcatggttt caaaagttca gctcattaac tgattggaaa ttgaacatca
     1141 cttatcctgc agcgcttcca ccccgggcag tttgcggccg gccataagct ccaggctggc
     1201 gccgccgccg gtactgacgt ggccgaagtg gccgaccagc ttgcgttctt ccaggtagcc
     1261 cacggtatcg ccgccgccca caacggtgaa cggcttgtgg ccgaactgtc cgagcagggc
     1321 gtctaccacc agttccgtac catgggcata agggccgacg ggtccctgca accctttggt
     1381 ttcggtgacg cccatggtgc cgttccacac gacggtgttg gccagttgga tgccgccggc
     1441 tatgaagctg ccgctgaagg gcccgatgtc caggatcatc tcctgccaca atacgtggct
     1501 ggcccgcgcc ggtacgcggt gcgggtagtt ttcgatgtcc gcgatgacgt gcgcgctcca
     1561 gtccactatc ctggtcgagg ccgtcttatc catcttgctt gccactacgc cgtcccgggg
     1621 gatggcaaag atgaagcgcc gctttctggc ctccgcccgc gccttatgca taatatcgcg
     1681 ggctaccggc agatcggctt tgtcgtacag actgtgagcc acgtctatgc cttcggcggc
     1741 caggaacgtg ttggccatgg cgccgccgac tgccacgaag tcggcaatgg caatgaaccg
     1801 gtgcagcagg tcgatcttat cggcaatctt ggcgccgccg attatcgcta ccagcggccg
     1861 ttccggcttt tccatgacgt tcgtaatggt gtcgacttct ttttcgagca gcagtcctgc
     1921 cacgctgggc agatggcggg taatggcagc ggtgctggca tgcgcccggt gcaccacccc
     1981 gaagccatcc tgcacgaaca cgtccgcgag gccggccagc agttgggcga agttatcgtc
     2041 gttttgctct tcctcggggt ggaagcgcag gttttccagc agtaacacct gccccgcctt
     2101 cag
//
LOCUS       DASSFD010000105         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1692152, whole genome shotgun sequence.
ACCESSION   DASSFD010000105 DASSFD010000000
VERSION     DASSFD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692152"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFH39_02415"
     CDS             <1..543
                     /locus_tag="VFH39_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924660.1"
                     /translation="GLRSKQGAPLSFTMYASDTAEHRLVAGMLKRQWRQVGVDCQTVF
                     QSDDDLSSTVATHSYDALLYGISIGVDPDVYVYWDSSQTDPRSTRLNFSMYKSGTADT
                     ALEAGRTRQDPVLRAVKYKSFLQAWQQDAPAVGLYQPRFLYLTNEKVYGLDAVSINTD
                     TDRFNNVVNWEVRTAKVTTH"
     gene            602..689
                     /locus_tag="VFH39_02420"
     tRNA            602..689
                     /locus_tag="VFH39_02420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:636..638,aa:Leu,seq:cag)
     gene            987..2624
                     /locus_tag="VFH39_02425"
     CDS             987..2624
                     /locus_tag="VFH39_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6924661.1"
                     /translation="MSKVAHYLQEHLVGEVVTSVDARRYFSTDGSVFSVPPAMIVYPR
                     SENDVRKTMRFAWQLAERGRVVPVTARGAGTDQSGAAIGSGIMLVFPAHMNRIIEFDS
                     KSGVLIVEPGINYGKLQQTLETHSRFLPPFPASFEYSTVGGAIANNASGEKSVKYGAT
                     DKYVQSLRVVLANGEVIETRRISKRELSKKLGLATFEGEIYRALDVLIEENQELIERM
                     RLPVTKNVAGYNLLDVKRKDGSFDLTPLLVGAQGTLGIITEATLKTEPYNPETTLLVA
                     HFDDVTAAQNAVQELRNFSEQPSAIEMVDGNLLEAVEAVNPNQLKDVINQPFPAVVLL
                     VEFDTPNDRQQKKYVKRATKVMERYATQHQIEQDPEKQAQLWKIRHASATVISQVQGR
                     AKAVPIIEDGVVPLAQFADYLRGTQEIFKRNGLQLAVWGHAGDANLHLQPYLDLSQVG
                     DRQKAFKVADEYYKLVISLGGSTSGEHNDGRIRAPYLSLLYGNEVYGLLQKVKQIFDP
                     YNSLNPGVKMNVSLEDIRPLMRHEYGLEHLYQHMPRS"
     gene            2939..3023
                     /locus_tag="VFH39_02430"
     tRNA            2939..3023
                     /locus_tag="VFH39_02430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2973..2975,aa:Leu,seq:gag)
     gene            3221..3297
                     /locus_tag="VFH39_02435"
     tRNA            3221..3297
                     /locus_tag="VFH39_02435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3255..3257,aa:Val,seq:tac)
     gene            3419..>3694
                     /locus_tag="VFH39_02440"
     CDS             3419..>3694
                     /locus_tag="VFH39_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924662.1"
                     /translation="MTRVARAEGPGPSDNFIPRPEITRRVLSAVAAADGEVISEQESV
                     PSADAALSTGNELISARSEDNSDSPEVEIEGSSVEEAETGDFEWDDEE"
BASE COUNT          869 a         1008 c         1054 g          763 t
ORIGIN      
        1 ggcctgcgta gcaagcaagg agcgccgctc agtttcacca tgtacgccag cgacactgcc
       61 gagcaccggc tggtggccgg catgctgaaa cgccagtggc gccaggtcgg cgtcgattgt
      121 cagaccgtct tccagtccga cgatgatctg tcctccacgg tggccaccca cagctatgac
      181 gcgctgctgt acggcatttc gataggcgtc gacccggacg tgtacgtcta ctgggacagc
      241 tcgcagacgg atccccgctc gacgcggctc aatttttcga tgtataagtc aggtactgcg
      301 gatacggcat tagaggccgg ccgcacgcgt caggacccgg tgctgcgagc cgttaagtac
      361 aagtcgttcc tgcaggcctg gcagcaggat gccccggctg tcgggcttta ccaaccgcgc
      421 tttttgtacc tgactaacga aaaggtatat ggccttgacg ccgtcagtat caataccgac
      481 accgatcgct tcaacaacgt cgtgaattgg gaagtccgca cggccaaggt gaccacccac
      541 taactcttgc ggattcgcta aaaaacagat acgatgtatg tcgtatcaaa ttcagtttcg
      601 tgcggatgtg gcggaattgg tagacgcgct agtttcaggt actagtgagc ttaacactcg
      661 tggaggttca agtcctctca tccgcaccaa gcaaatgccc cagctagtct ggggtttttg
      721 cttggtcgcg attaggagtc ccatggcaga tgttgcgtag caaaagatgt gagaggatgg
      781 tcgcgcgcag ctgagctctc acagggatat agtccgagaa agcctgagcg ggaaaggacg
      841 acgtccgctc gtcactccag gcacaaaccc taagaagcta ccacttcaag agtaaatcta
      901 cctcacccct gtccctcagc gtatccgtaa gcgttatgct attattacga atagaaaaat
      961 gaaacataca acgagtgagg tcagccgtga gtaaggttgc acattattta caggaacatc
     1021 tggtcggtga agtcgtgacc agcgtcgacg cccgtcgcta tttctcgacc gacggtagcg
     1081 ttttttcggt gccgccggct atgattgtct atccgcgcag cgaaaacgac gtccgtaaga
     1141 cgatgcgctt cgcctggcag ttggcagagc gcggtcgggt cgtcccggtc accgctcgtg
     1201 gcgccggtac ggatcagtcc ggggcggcta tcggcagcgg catcatgctg gtgtttcccg
     1261 cgcacatgaa ccgcatcatc gagttcgaca gcaagtccgg cgtactcatc gtcgaaccgg
     1321 gtatcaacta cggcaagctg cagcagactc tcgagacgca tagccgcttc ctgccgccgt
     1381 tcccggctag ttttgaatat tcgaccgtag ggggcgccat agccaacaac gccagcggcg
     1441 agaagtcggt caagtacggc gctaccgata agtatgtgca aagcttgcgc gtggtcttgg
     1501 cgaacggcga agtcatcgaa acgcgccgga tcagtaaacg cgaactcagc aaaaagctcg
     1561 gcctggcgac gttcgaaggc gaaatctacc gcgccctgga cgtgctgatc gaggaaaacc
     1621 aggaactgat tgagcggatg cgcctgccgg tgaccaagaa tgtggccggt tacaacttgc
     1681 tggacgtgaa gcgcaaggat ggctcgttcg acctgacacc gctgttggtt ggcgctcagg
     1741 gcacgctagg tattatcacc gaggccactc tcaaaacgga gccatataac cccgaaacaa
     1801 cgctgctagt cgcgcatttc gacgatgtga ccgccgcgca gaacgccgtc caggagctcc
     1861 gcaacttctc tgagcaacct tcagccatcg aaatggtcga tggtaacctg ctagaagctg
     1921 tcgaagccgt caaccccaac cagctgaagg atgtcatcaa ccagccgttc ccggctgtcg
     1981 tgctgctcgt ggagttcgac actcccaacg accggcagca gaagaagtac gtcaagcggg
     2041 cgactaaggt catggaacgc tacgctaccc aacatcagat tgagcaagac cccgaaaagc
     2101 aagcgcaact gtggaagatc cggcatgcct cagctaccgt gatatcgcag gtgcaggggc
     2161 gtgccaaagc cgtaccgatt atcgaggacg gagtggtacc gctggcccag ttcgccgact
     2221 acctgcgcgg tacgcaggag atattcaaac gcaacggcct gcagcttgct gtctggggcc
     2281 acgccggcga cgccaacctg cacctgcagc cctaccttga tctcagccag gtcggcgacc
     2341 gtcagaaagc cttcaaggtg gccgatgagt attacaaact ggttatcagt ctcgggggca
     2401 gcacgtccgg cgagcacaat gacggccgca tacgcgcgcc gtacttgtcg ctgctatacg
     2461 gcaacgaagt ctatgggctg ctgcagaagg ttaagcagat attcgacccg tacaacagcc
     2521 tgaaccctgg cgtgaagatg aacgtcagcc tggaggacat ccggccgctc atgcggcacg
     2581 agtacggttt ggagcatctg taccagcaca tgccccgttc gtagcgacgg cctgccttgc
     2641 tggcacattt cgcccgagta gctccagtcc gcgctcaccg tacgccaagt acggttcgct
     2701 tacgggcttc gctcttcggc caaactgcac ccagccagga aggcctttcg gcagcgcaac
     2761 cccttgagtt ttagggcaaa aacagataag ataacttggt tcatttacaa gtgatgttta
     2821 caaaagaccg aaccagggag cgtagtcgac cgttcgggct tttgagagga gaagcatagc
     2881 cgtatgtcga agcttttgcc gtcaaggcaa aacgagacag cggcgtaagc ttcgacgagc
     2941 ggacgtggcg gaattggtag acgcgctact ttgaggtggt agtcccgcaa ggggtggagg
     3001 ttcaagtcct ctcgtccgca ccagtaacga gacacccgta aacgggtttc tcatcgcgcc
     3061 gggaaatgaa atgaattcat ttcgcgggct gctcttcccc cagctaagct ttggctaaaa
     3121 gagtgggggg cgtaaatccg aatttatttc gaatttacgg ctgccgctta gggaaaagag
     3181 cagcgcagcg tcgagaaaaa ccgcaggttt gtcttgaagt gggtgattag ctcagttggc
     3241 tagagcacct cgtttacacc gagggggtcg ggggttcgag cccctcatca cccaccatat
     3301 aaaacggcta tgctgcatcg gcatggcctt tttatgttat gaacagattg ggtcgtcata
     3361 aacatgacac atattacgct tgcactttag atattttgta tgcataataa tgcaaaccat
     3421 gaccagagta gcacgggcgg aaggccctgg cccttccgat aactttattc cccggccaga
     3481 gataacacga agagttttgt cggctgtagc tgccgcagac ggtgaagtaa tctcggagca
     3541 agaatctgtg ccgagtgctg atgcggctct ttctaccggt aatgaactta tatcagcacg
     3601 ctcagaagat aatagtgatt ctcctgaagt cgagattgag ggtagcagtg ttgaggaggc
     3661 ggaaactggt gattttgagt gggatgacga ggaa
//
LOCUS       DASSFD010000106         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_187004, whole genome shotgun sequence.
ACCESSION   DASSFD010000106 DASSFD010000000
VERSION     DASSFD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187004"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..526
                     /locus_tag="VFH39_02445"
     CDS             155..526
                     /locus_tag="VFH39_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924663.1"
                     /translation="MKTNLLFAATLASMAITGTGAGVAGAVSSSNTPPRNRIARYTIR
                     EERLNVEAEVLGTTPSDLEDKLKTETMEQVITSAGLDKSTFRHKVHAKLQSDLLAKGY
                     SWDQINRLLNRPGRGRAPSTA"
     gene            complement(636..711)
                     /locus_tag="VFH39_02450"
     tRNA            complement(636..711)
                     /locus_tag="VFH39_02450"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(676..678),aa:Pro,seq:cgg)
     gene            complement(893..1015)
                     /locus_tag="VFH39_02455"
     CDS             complement(893..1015)
                     /locus_tag="VFH39_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924664.1"
                     /translation="MGNYLEDAEKRLDEAAEDYKETLKKKKKTNNSGNPPRIAV"
     gene            1231..1857
                     /locus_tag="VFH39_02460"
     CDS             1231..1857
                     /locus_tag="VFH39_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924665.1"
                     /translation="MPLVFTVAAVLTLLLANEWWWRRSSVHGELGRKFVHITVGTFVA
                     FWPYYLSWNQIRFMSVAFLLVVGLSKYFNIFRAIHSVTRPTLGELYFGLAVGLTTFVT
                     DRPALYAAALLQMSLADGLAAVIGVRFGRGNGYRVLGALKSLAGSGTFLLTSYVILLS
                     LSVFSTHLAIPYCLGIAVAAAVLENVGLRGLDNLLVPLFVAGMLRLLV"
     gene            complement(1854..>2276)
                     /locus_tag="VFH39_02465"
     CDS             complement(1854..>2276)
                     /locus_tag="VFH39_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET6924666.1"
                     /translation="GINDVYAEALPGEKVHSIEQVSERPVVFVGDGVNDAPVLTAADV
                     GIALGARGSTVASESADIVIMLDDLSRVAVATRIARRTFAIARQSILIGIFISLALMV
                     MFATGKFPPLLGAILQEVVDVVVIFNALRAHLSGRQAA"
BASE COUNT          444 a          650 c          647 g          535 t
ORIGIN      
        1 ctttccccgt acgcgggatc aggtacggaa cggcgcgccg gttggctttg ccggattctg
       61 ccgtcgagcc cgggacagtt ctgactaccc cttcagttcg gcttcagttg cccgtcatat
      121 tgtgagagtg tcagatgcaa aaggagggta aattatgaag accaatttac ttttcgctgc
      181 caccttggcc agcatggcta taactggtac gggtgccgga gttgccggcg ctgtctccag
      241 ttcaaatacg ccgccccgta accgcatcgc ccgctacacg attcgcgagg agcgtttgaa
      301 tgtcgaagcc gaggtgctcg ggacgacgcc atcggatctg gaggacaagc tcaaaacgga
      361 aaccatggag caggtcatca ccagtgccgg cctcgacaaa agcaccttcc gtcataaagt
      421 acatgcaaag ctgcagagcg atttgctggc gaagggatac agctgggacc agattaaccg
      481 gctgctgaac cgccccggtc gggggcgggc acccagcact gcataatggt tggcgatcct
      541 agcctcatcc cttccaatct tccaaattca aaaacaagct tagtgcggcg gcgacggggc
      601 agacgggcgg cgcgtcgcgg gatacggaat aatcttggtc ggggtgatcc gacttgaacg
      661 gacgacctca gcgtcccgaa cgctgcgcgc taccaactgc gccacacccc gaaagcgcca
      721 ggggcacttt gggtagacgc gaaggaaccg ccgctagtgc aacctaatct ggcatgggcc
      781 tgtaatgtac gaaatgccct gggacatttt gtagccgcga atgaaacgtc gtgcataaaa
      841 gcacggttga tgccgtacgc gagccacaga ttacaaaatg taacaggggt gattatacgg
      901 caatgcgcgg tggattgcca gagttattcg tcttcttctt tttctttagc gtttctttgt
      961 agtcttccgc cgcctcatcg agccgctttt cggcgtcttc caggtaattt cccattcgtc
     1021 attcctccga ttccacttct tctttctttt cttcggcctt ttcctcttgc tttttagcga
     1081 attcctcggc ctgctctgcg gcccggtcgc ctatgccatc cagtgctccc atattgcctc
     1141 ctctcttctt atcttttcat ggtagactca gccaacaaaa tacactgtcg taatttttac
     1201 gagcagcagt gacgtacaat ggtagtaatc atgccactcg tgtttaccgt cgccgccgtg
     1261 ctcacgctgc tgctcgccaa tgaatggtgg tggcgccgaa gttccgtgca cggtgagctc
     1321 ggacgtaagt tcgtgcatat aaccgtcggt actttcgtgg ccttctggcc gtattacctg
     1381 agctggaacc aaatacgctt tatgagcgtg gccttcttgc tggttgtggg cctgtccaaa
     1441 tacttcaata tcttcagggc tatccacagt gtgacccggc ccacgttggg cgagctgtac
     1501 tttggtttgg cggtcgggct cactacgttc gtgaccgata ggccggcact atatgctgcc
     1561 gcgctgctgc agatgagtct ggcggacggc ctggcggcgg taatcggtgt ccgcttcggc
     1621 cgcggcaacg gttaccgggt gctcggcgca ttgaaaagtc tggccggatc gggaacattc
     1681 ctgttgacga gctatgtgat acttttgagc ctgtcggtct tcagtacgca tcttgccata
     1741 ccatactgcc tgggaatcgc ggtcgcggcg gcagttctcg agaatgtcgg gctgcgcggt
     1801 ctggacaacc tgctggtgcc gctgtttgta gccggtatgc tgcgactact ggtctaagcg
     1861 gcttgcctgc cgctgaggtg agcccgcaag gcattgaata tgactacgac gtccaccact
     1921 tcctgcagga tggcgccgag caagggcggg aacttgccgg tggcgaacat caccatgagt
     1981 gccaggctta tgaagatgcc tatgagaata ctctggcggg caatggcgaa agtgcgcctg
     2041 gctatgcgtg tggcgaccgc tacgcggctt agatcgtcca gcatgataac tatatcggcc
     2101 gattcgctgg cgaccgtgga gccgcgggcg cccagggcga taccgacatc agcggcggtc
     2161 agtaccggcg cgtcgttcac gccgtcaccc acgaacacca ctggccgctc gctgacctgc
     2221 tctatggaat gcaccttttc gcccggcagc gcctcggcat atacatcgtt gatgcc
//
LOCUS       DASSFD010000107         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_409885, whole genome shotgun sequence.
ACCESSION   DASSFD010000107 DASSFD010000000
VERSION     DASSFD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409885"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..706
                     /gene="secE"
                     /locus_tag="VFH39_02470"
     CDS             272..706
                     /gene="secE"
                     /locus_tag="VFH39_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET6924667.1"
                     /translation="MDPSQPSRKKRRLRAPAETVREKAEKAGAEAQAQAAQDNPHRLR
                     KFFSFIFSPLRWLSHKPPLKQIGHGLRWFFTRRFMRFLGRLLGLRYLRDSWRELKLVA
                     WPSRRQSRQLTFAVIIFSVIFGGLIAIVDYGLDKLFKHIILK"
     gene            721..1269
                     /gene="nusG"
                     /locus_tag="VFH39_02475"
     CDS             721..1269
                     /gene="nusG"
                     /locus_tag="VFH39_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET6924668.1"
                     /translation="MSTSKRYDTTKQWYAIHTYSGYEEKVAESIRQRADSLDMKDKIF
                     EVLVPKEKMIEIKNGKRRVVEKRIFQGYVLVQMKMSEDAWFIVRNTPSVTGFVGSGAD
                     PTPVGQDEIEKIQKRMGLEQPKHKIDYAIGEVVSITDGPFKGFDGSINEIDAQKGKLK
                     VLVNMFGRETPVELDSLQVKRV"
     gene            1298..1609
                     /locus_tag="VFH39_02480"
     CDS             1298..1609
                     /locus_tag="VFH39_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924669.1"
                     /translation="MPDRLSLIAKAERATKLAARNGAARVGAEILFFYSIWGSLVEAA
                     DVPGANNAFKFAAIATGSAAIAGYNEIRETMHETQAIKAREQAVQAFRRDLRPEQSES
                     E"
     gene            1758..1967
                     /locus_tag="VFH39_02485"
     CDS             1758..1967
                     /locus_tag="VFH39_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924670.1"
                     /translation="MLEGMGIMGLGEATSNPAAVSVALAVGAVAAGGGILLIGRGAEQ
                     IEQFGSAEPSPNDEMDVSEGQGDVE"
     gene            1964..2251
                     /locus_tag="VFH39_02490"
     CDS             1964..2251
                     /locus_tag="VFH39_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924671.1"
                     /translation="MSRQATAYEHYDYGSPTYYRETAWALRLGAKLMAAGGIFVGVAF
                     GVDVADGHVTLKNESDIVQIGAMDIVAAAAFEMGNTLQLRKARAAENNLEQ"
     gene            2387..>2710
                     /locus_tag="VFH39_02495"
     CDS             2387..>2710
                     /locus_tag="VFH39_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET6924672.1"
                     /translation="MAKAVKANLKMKIKGGQASAAPPVGSTLGQYGVNMMDFIGPFND
                     ATKDMMGKTVTAHITIYDDRTMDFRVVAEPTDDRIRAALGIQKGSGRPNSEKISKKLS
                     QADLRK"
BASE COUNT          709 a          704 c          755 g          542 t
ORIGIN      
        1 cagcaaataa agccacactg acaaccagca gaccgatcat cagcggcggc agcagccatg
       61 cccgctgttt ccagagtaac cgtgtgtatg ccagccactc tacaaaagac agcgccacca
      121 ggcagataag cataacggtc attacaagaa actatagcac gctacccagc cgcatgactt
      181 ccattgaccc tcgtacgctc cctgtgctat attgacaaca acgacctaca tagcaatgta
      241 ggttttttaa ttttcaggag actaaacgtc agtggacccc agtcagccat cacgtaagaa
      301 acgccggtta cgcgcgccgg ccgaaaccgt gcgcgaaaaa gccgaaaagg ccggtgccga
      361 agcgcaagcc caggcggcgc aggacaaccc tcaccgttta cgtaagtttt tcagcttcat
      421 tttctcaccg ctgcgatggc tgagccacaa accgccgctc aaacagatcg gccacggcct
      481 gcgctggttc tttacgcgcc gtttcatgcg cttcctgggc cgcctgcttg ggctgcgcta
      541 tctgcgtgac agctggcgtg agctgaagct ggtcgcctgg ccgagccggc gccagtctag
      601 acagctgacg ttcgctgtca tcatcttttc ggtcattttc ggcggtctta tcgccatcgt
      661 cgactacggt ctcgacaaat tgtttaaaca catcattctc aagtaaggag ttataggagt
      721 atgtctacca gcaaacgtta cgataccacc aagcagtggt acgccatcca cacttacagc
      781 ggttacgagg aaaaggttgc cgagagcatc cgccagcggg ccgacagttt ggacatgaag
      841 gataagatct tcgaagtcct cgttccgaag gaaaaaatga tcgaaatcaa gaacggcaaa
      901 cgccgcgtcg tcgagaagcg cattttccag ggttacgtac tggtacagat gaaaatgagc
      961 gaagacgcct ggttcattgt gcgtaacacg ccgagcgtca ctggcttcgt cggtagcggc
     1021 gccgatccga caccggtcgg ccaggacgag attgagaaga tccagaaacg gatgggcttg
     1081 gagcagccga agcacaagat cgactacgcc atcggtgagg tcgtcagtat taccgacggg
     1141 ccgttcaagg gcttcgacgg ctccatcaac gaaatcgacg cccagaaggg caagctgaag
     1201 gtactggtca acatgttcgg ccgcgaaaca ccggtcgaac tcgacagcct gcaagtaaag
     1261 cgcgtctaac actaggcaga aaaaaatgac ctcacgcgtg cccgaccgac tatcgctaat
     1321 agccaaagca gaacgtgcga cgaagttggc agcccgcaac ggagccgcca gggtgggagc
     1381 cgagatactg ttcttttatt ccatatgggg ttctctggtc gaagcggcag acgtaccggg
     1441 cgccaataat gcgttcaaat ttgcggccat cgctaccggt tctgcagcca ttgccggcta
     1501 caacgaaata cgtgagacga tgcacgagac gcaggcaata aaagcacgcg agcaggctgt
     1561 acaagcgttt cgccgtgact tgaggcctga acagagtgaa agtgagtaga tgacggcgct
     1621 cacgcttggt gttatcatcg tataattaag cactggcaaa agaaggaaac aaggttatgg
     1681 caagacggcg aagctacgac acggataact caactcgaga tatgttgggc ggcgtaacgg
     1741 gtatagctgt aggctgcatg ttagaaggca tgggcataat ggggttggga gaggccacca
     1801 gcaaccctgc cgcagtatcg gtagcccttg cggtcggcgc cgtcgcggct ggaggcggga
     1861 tattgctaat tggccgaggt gctgagcaga ttgagcagtt cggcagtgca gagccttcgc
     1921 cgaacgatga aatggatgtt tcggaaggtc aaggagatgt cgaatgagcc gccaagcaac
     1981 tgcttatgaa cattatgact acgggagtcc gacctattac cgtgagactg cgtgggcgct
     2041 acgccttggc gcaaaactaa tggctgccgg cggaatattt gtcggtgttg cgtttggtgt
     2101 tgatgttgcg gatggtcacg tgactcttaa aaatgagtca gatatcgtgc aaataggagc
     2161 tatggatatc gtggcggccg ctgcctttga aatgggaaat acgcttcagc ttcgcaaggc
     2221 gcgagcagcc gaaaataatc tggagcaata agaagggctg gtttcgcttc aatcgcgtga
     2281 caactctgac ctaaactgat ataatcacat ctgttacgca ctacttaaag gtaggagaac
     2341 cagtaaccag cttcataagg tgaacgttcg aaaggagtta taacttatgg ccaaagcggt
     2401 caaagctaat ttgaaaatga agatcaaggg tggccaagcc agtgccgcgc caccggtcgg
     2461 tagcaccctc ggtcagtatg gtgtgaacat gatggacttc atcggtccgt tcaatgacgc
     2521 caccaaggac atgatgggta agaccgtcac tgcccacatc actatttatg acgaccgtac
     2581 gatggacttc cgcgtcgtcg ctgagccgac cgatgaccgt atccgcgcag ctctcggcat
     2641 ccagaagggc tccggccgtc cgaacagcga aaagatcagc aagaagctga gccaagccga
     2701 tctgcgcaaa
//
LOCUS       DASSFD010000108         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1788963, whole genome shotgun sequence.
ACCESSION   DASSFD010000108 DASSFD010000000
VERSION     DASSFD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788963"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFH39_02500"
     CDS             <1..940
                     /locus_tag="VFH39_02500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6924673.1"
                     /translation="LGLGCMGMSAFYGTGDEQESIEVIHYALDHGINFLDTADMYGPW
                     TNEKLVGKAIKDRRDQVVLATKFGNEVDEEGNRTGKINGRPDYIRKCIEGSLQRLGVD
                     HIDLYYQHRVDPDVPIEDTIGAMADLVKEGKVRYLGMSEAAPETIRRAAKVHKITALQ
                     TEYSLWTRDPEDEILQTVRELAIGFVAYSPLGRGFLTGQIKSPEDLEEGDFRRQNPRF
                     QGENFQKNLELVDKVKEIANEKGCTPGQLALAWVMAQGEDIVPIPGTKHVKYLQENLG
                     AVDVALTDEDLKRLDEVAPKGATAGDRYPDMSTVNR"
     gene            1026..2129
                     /locus_tag="VFH39_02505"
     CDS             1026..2129
                     /locus_tag="VFH39_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924674.1"
                     /translation="MAEVPHILSARQFDEAQIAEIFERTNAMYAAFENDRRALASRHV
                     GYVLSTSFYEASTRTRKSHEAAAAQLGMGVITTENAKEFSSAVKGETIRDTTLTEAQL
                     CDVILMRHYEEGAADIAAEVSPVPIINGGDGPGEHPTQALLDLYTIYKEKGRTDDLHV
                     VIGGDLIKGRTARSLAMLLSHYKNNHITFVSLPELRIDERVKSHLSEAGISFEETTEM
                     YDALPQADVVYWTRLQLERHARKTPADEAAVLRALQKIGARAAGAVLDSVKHNAAQAL
                     VEIGAISGEILDADNTQEEMSAVSLARNYVIDQAALQAMKGNAIIMHPLPRNNEITET
                     VDSDPRAKYFVQVRHGKFLRMALLDQLLEKTAA"
     gene            complement(2166..2804)
                     /locus_tag="VFH39_02510"
     CDS             complement(2166..2804)
                     /locus_tag="VFH39_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6924675.1"
                     /translation="MSLLATPEELQKAANHLSEADAVLAPVIAQHGLARLEPHTDYYQ
                     ELVDSIISQQLSVHAARAIEQRFRDLFGGHFPAPAEILSTDAEALRGIGFSYAKARYV
                     LDLAQHIVDGKVRFDHLDALSNEEIIAELTAVKGIGEWTVHMFLMFCMGRLDVLPVGD
                     LGIRNGIRKLYGFNDAPTPQQIRDIAEQYSWHPYESAASWYVWQSLDNAPAL"
     gene            3029..>3291
                     /locus_tag="VFH39_02515"
     CDS             3029..>3291
                     /locus_tag="VFH39_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924676.1"
                     /translation="MQNDKKRRGIANEALTPDEEKELESRVDQMMQLERERADKSVPA
                     EATDMPKQPEATADTSPVDVPSAPPLATAESVEASPAEPTDTAP"
BASE COUNT          807 a          866 c          946 g          672 t
ORIGIN      
        1 actcggtttg ggctgcatgg gcatgagtgc tttttacgga accggcgacg agcaggaatc
       61 gatcgaggtt atacactacg cgctggacca tggcatcaac ttcttggaca cggccgacat
      121 gtacggcccg tggaccaacg agaaactggt cgggaaggcc ataaaggacc ggcgcgacca
      181 agtggtactg gcgaccaaat tcggcaacga agtagacgag gaaggcaacc gtaccggcaa
      241 aataaacggc cgccccgatt atattcgcaa gtgcattgaa ggcagcttgc agcggctcgg
      301 tgtcgatcat attgacctgt attaccagca ccgcgttgac ccggatgtgc ccatcgagga
      361 caccatcggc gccatggccg atctggtgaa ggaaggcaag gtccgctatc taggcatgag
      421 cgaggccgcg ccggaaacta tccgccgggc ggccaaggtg cacaagatta cggcgctgca
      481 gacggaatac agcttgtgga cgcgcgaccc ggaggacgaa atactgcaga ccgtgcgcga
      541 acttgctatc ggctttgtgg cttacagccc gttgggccga ggttttctga ccggacagat
      601 caaaagtccg gaggatttgg aagaaggtga cttccgtcgc cagaaccctc gcttccaggg
      661 cgagaacttc cagaagaatc tggagctggt agacaaggta aaagaaattg cgaacgaaaa
      721 gggctgcacg cccggacagt tggcactcgc ttgggtaatg gctcagggcg aagatatcgt
      781 gccgatccct ggcactaaac atgttaagta tttgcaggag aacctcggcg ccgtcgacgt
      841 cgcattgacg gacgaggacc taaagcgtct agacgaagtg gctcccaaag gtgccaccgc
      901 cggcgaccgc tacccggaca tgtccaccgt caaccgctag gcgttgtaaa gttaggcaac
      961 tttattgccc ggaaagcata agctgttgta ttgttatggc aacttaagtg gagggtcccc
     1021 gcgccatggc agaagtaccg cacatccttt cggcacggca attcgacgaa gcgcaaattg
     1081 cagaaatttt tgagcgcacc aatgcgatgt atgcagcttt cgaaaatgac cgtcgggcct
     1141 tggcatcaag gcatgtcggc tatgttctgt cgacgagctt ttacgaggcg tcgacccgca
     1201 cgcgaaaaag tcatgaagcc gccgcggctc aattagggat gggggttata accaccgaaa
     1261 atgccaaaga attttcgtcg gcagtcaaag gcgagaccat tcgcgatacg acgctgactg
     1321 aagcgcaatt gtgcgatgta atcctcatgc gtcactacga ggaaggtgct gctgacattg
     1381 cggctgaggt ttcgcccgta ccgattatca atggtggtga tgggcctggc gaacacccga
     1441 cacaggcgtt acttgatcta tacacgatat ataaggaaaa aggacgtacc gatgacctgc
     1501 atgtcgttat cggtggcgat cttatcaaag ggcgaacagc aagatcgctc gccatgcttc
     1561 taagtcacta caagaacaat cacattacat tcgtatctct tcctgagcta cgcatcgacg
     1621 aacgcgtaaa atcacatctg tcggaagcgg gcatatcgtt tgaagaaaca accgagatgt
     1681 atgatgctct gccacaggca gatgtggtgt attggacccg tttacagctt gaacggcatg
     1741 ctcgtaaaac gccggcggac gaagcggccg tcctaagggc gttgcaaaaa attggcgcaa
     1801 gggcagcggg tgctgtactg gactcagtaa agcataatgc cgcacaagct ttggttgaaa
     1861 taggtgcaat atccggggaa attctggatg ccgacaacac tcaagaagag atgtctgccg
     1921 tcagtcttgc aaggaattac gttatcgatc aggctgcact acaggcaatg aaggggaatg
     1981 cgattatcat gcatccactt cctcgcaaca atgaaattac cgaaacggta gatagcgacc
     2041 ccagggccaa gtactttgtg caggttcggc acggaaagtt cctgcgtatg gcactactcg
     2101 atcaattgct cgagaaaacc gcagcctagg ctttgagaat tccttatttt gaggggcgct
     2161 cctgtttaca gcgccggggc gttgtctagg gattgccaaa cgtaccagct ggcggctgat
     2221 tcgtaggggt gccagctgta ttgctcagcg atgtcgcgga tctgctgcgg ggtcggcgca
     2281 tcgttgaagc cgtagagttt gcggatgccg ttgcggatgc cgaggtcgcc gaccggcagc
     2341 acgtccagcc gacccatgca gaacatcagg aacatgtgca ccgtccattc gccgatgcct
     2401 ttgacggccg tcaattcggc gattatttcc tcgttgctca atgcgtccag gtggtcgaac
     2461 cgcactttac cgtcgacgat gtgctgcgct aagtccagca cataacgggc tttggcgtaa
     2521 ctgaaaccga taccgcgcaa agcctcggcg tcggtgctca gaatctctgc gggagcaggg
     2581 aaatggccgc cgaacaggtc tcggaaacgc tgctcgattg cccgggcggc gtggacgctc
     2641 agctgctgac taatgatact gtcgaccagc tcctggtaat agtcggtatg cggctccagc
     2701 cgtgctaatc catgctgggc aatcaccggc gccagtacgg cgtcggcctc ggataagtga
     2761 ttggcagcct tttgcagttc ctcaggcgtg gcgagcaagc tcatagcctg attgtaacag
     2821 cgtggggtgt catccgaaga agatatcgcg aataatgctt cgcttgacgg ctttcttcac
     2881 tgggtggccg tctttgtcgg tctggtcgtc tgccatagta ctacctcctg gctgcgtatg
     2941 ataccgttcg ccggatagcc gcgactgtaa gatttccggc tgagtggcga ttacagcccc
     3001 cgatttgata caatacccct gaaagcatat gcaaaatgac aaaaaacggc gcggcatagc
     3061 caacgaagcg ttaaccccgg atgaagaaaa agaacttgag tcccgggttg accagatgat
     3121 gcagctcgaa cgggagcgtg ccgataaatc agtaccggcc gaagctacag acatgccgaa
     3181 gcaaccggag gcaaccgcag atacaagccc cgtagatgtc ccctccgcgc caccgctggc
     3241 caccgctgaa tcggtagaag catctccggc cgaaccaacc gacacggctc c
//
LOCUS       DASSFD010000109         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1076537, whole genome shotgun sequence.
ACCESSION   DASSFD010000109 DASSFD010000000
VERSION     DASSFD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076537"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFH39_02520"
     CDS             complement(<1..903)
                     /locus_tag="VFH39_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HET6924677.1"
                     /translation="MKKQHLISTVTTFFVATVVAMSLVSHKALAATGQIYLSPASLSV
                     QNGNNVTLNLRINPGTTINGVQATVNFDTSALQYVSVSTASSAFSVQLQQSQSGGAIT
                     VARGDLSGGISSDSLVESITFKALASSGTSSVTLSGANATDQATSTYTNPSVVNATVN
                     FTAGSCPDGQTGTPPNCTTPTPPPSGGGTGGGSGGGSGSTKSGGSGNTGSNGGSKKPV
                     TKPSSGTEATPTVVGAPATQFTTASVSATTQVPTQVYLIYGTNANTLDQKTALSSFAT
                     SHNISLADAHLTPGETYYYIVVSKD"
     gene            complement(1019..1576)
                     /gene="orn"
                     /locus_tag="VFH39_02525"
     CDS             complement(1019..1576)
                     /gene="orn"
                     /locus_tag="VFH39_02525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET6924678.1"
                     /translation="MTIDKHAIPTKLLWVDLEMTGLDAQNDVILEVAAEITDFNFRTL
                     ATYEACVKQPRDVVVDRMQKNKWWADFPANRDSFVEKLDDGKDPAEVERELCALIGEH
                     FGDEPAVLAGNSIHNDRNFIKQWWPQLDLKLHYRMLDVSSLKLLMQGKYGVHFEKKEI
                     HRAFDDIQASIAELQYYLEWFQDRR"
     gene            complement(1601..2227)
                     /locus_tag="VFH39_02530"
     CDS             complement(1601..2227)
                     /locus_tag="VFH39_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6924679.1"
                     /translation="MQVTVFGASGKVGHLVVEELLARGHTVVAFIHRRQLFVPNNRLI
                     IKTGDIYDAAAVAEALRGSDAVISCLGSWGPKGRDVLSAAMRNIIPAMREQKISRIVT
                     LTGSGATAPQEKTGVGHGLIMKVFRPFPAGKVFNDGEEHMRLLAASGLDWTTVRSPVM
                     TNFGGASYRLDVGPGSQLRPIARAAVANALVDQLDDTRWLHRAPVIYQ"
BASE COUNT          459 a          689 c          634 g          551 t
ORIGIN      
        1 gtccttcgat accacgatgt agtaataggt ctcccccggc gtcagatgcg cgtctgccaa
       61 gctgatgttg tgggaggttg caaacgagct cagcgctgtc ttctgatcaa gggtattggc
      121 gttggttccg tagatcagat agacctgcgt cgggacctgg gtggtagcgg aaacactggc
      181 cgtggtgaac tgggttgccg gcgcaccgac gaccgtcgga gttgcctccg taccggacga
      241 aggcttcgtc accggtttct tgctaccccc gttagagccg gtgttaccgg aaccaccgga
      301 cttggttgat ccggaaccgc cacccgagcc gccacccgtt ccaccaccgc tcggtggagg
      361 cgtcggtgta gtgcagttcg gcggcgtacc ggtctgaccg tccggacagc tgccggctgt
      421 gaaattgacc gtcgcgttga cgacggatgg gttagtgtac gtggacgttg cctgatccgt
      481 ggcgttcgcg ccggaaagtg tgacgctgct ggttccgctg ctggccaatg ccttgaacgt
      541 tatgctctcg accagcgaat cgctactgat tccgccagat aagtcgcccc gcgccacggt
      601 gatggcgccc ccgctctgtg attgctgcaa ctggaccgag aaggccgaac tagcagtgct
      661 gacgctcaca tattgcaggg ccgaggtatc gaaattcacc gttgcctgca cgccgttgat
      721 agttgtcccc ggattgatcc ggaggttcag cgtcacatta ttaccattct gtacacttag
      781 actggctggc gacagataaa tctgtcctgt cgcggccaac gccttatgac tgaccaggct
      841 catagccaca accgtagcca caaagaaggt ggttacggta gatataagat gttgtttttt
      901 cattttttgg taccgttact attccttaat gtaaacgtta ccgctttgct taaaatttta
      961 caatactttt tgtacgtgat ctatgacaca tatggtacga ccgataaatc agtaccagct
     1021 accgcctgtc ctggaaccat tccaggtagt actgcagctc ggcgatgctc gcctggatat
     1081 catcgaaggc gcggtgaatt tctttctttt cgaagtggac gccgtacttc ccttgcatca
     1141 gtagcttcag ggacgaaaca tcaagcatcc ggtagtgcag cttgagatcc agctgcggcc
     1201 accactgttt aatgaagtta cggtcgttgt ggatgctgtt cccggccaat accgccggct
     1261 cgtctccgaa gtgttcaccg ataagcgcac atagctcccg ttcgacctcg gccggatctt
     1321 taccatcatc caacttctct acgaagctgt cgcggttggc cggaaagtcc gcccaccact
     1381 tgttcttctg catccggtcc actaccacgt cgcgcggttg cttgacgcaa gcttcgtagg
     1441 tagccagggt gcggaagtta aagtcggtga tttcggctgc cacctccaag atgacatcgt
     1501 tctgggcatc cagcccggtc atctccaaat ccacccacag cagcttcgtt ggtatggcat
     1561 gtttgtctat ggtcattgct gtcattatac cgtacggtac ttattgataa atcacggggg
     1621 ctcgatgcag ccagcgagta tcgtcaagct gatcgacgag ggcgttggca actgcggcgc
     1681 gggcaatcgg gcgcagctga gaccccggcc cgacgtccaa cctatacgaa gcgccgccga
     1741 aattggtcat gaccggcgaa cgtaccgtcg tccaatccag cccgctggcg gccagcagac
     1801 gcatatgttc ctcgccgtcg ttaaagacct tgccggccgg gaacggcctg aacactttca
     1861 tgataagtcc gtgtcctacc cccgtttttt cttggggcgc agtcgcgccg ctaccggtca
     1921 gtgtgacgat acggctgatt ttttgttcgc gcatcgccgg aatgatgttc cgcatggcgg
     1981 cgctgagtac atccctgccc ttgggtcccc agctgcccag acagctgatg accgcgtcgc
     2041 tgcctcgcag cgcttccgcg actgccgccg cgtcatatat atcacccgtc tttataatga
     2101 gccggttgtt cggcacgaac agctgccgcc ggtggatgaa cgccaccacg gtatggcctc
     2161 gtgccaagag ctcctcgacc actaaatgcc cgaccttacc gctcgccccg aaaaccgtca
     2221 cttgcatata tataagtata aagtaagccc cgaccgctag tccggtcggg gcctcacgcc
     2281 taagtctctc aaaacttatc aggcttggac ggctgcttca gccgctcgcg cgg
//
LOCUS       DASSFD010000110         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1543895, whole genome shotgun sequence.
ACCESSION   DASSFD010000110 DASSFD010000000
VERSION     DASSFD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543895"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFH39_02535"
     CDS             complement(<1..481)
                     /locus_tag="VFH39_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET6924680.1"
                     /translation="MNDYSGKPTAQILVPTVIENEGRYERAYDIYSRLLKDRIVFLGS
                     DVNEATANLVVAQLLFLQAEDDKKDIYFYINSPGGSVYDALAIYDTIQYVTNDVQTVG
                     IGVQASAAAFLLSSGTKGKRFILPHATVMIHQPSSGTRGKVTDQEIDLRESLRIKKLL
                     "
     gene            complement(532..717)
                     /locus_tag="VFH39_02540"
     CDS             complement(532..717)
                     /locus_tag="VFH39_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924681.1"
                     /translation="MLEWLYKAPEQAALDSCPDDQEAHEDLIARRPDLALKHGLIPMQ
                     RAVVLGLMADPENKQGM"
     gene            complement(710..1999)
                     /gene="tig"
                     /locus_tag="VFH39_02545"
     CDS             complement(710..1999)
                     /gene="tig"
                     /locus_tag="VFH39_02545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET6924682.1"
                     /translation="MQVALEKLNPTKFKLTITADSELLLQVKGHVLGDLAKQVKLPGF
                     RAGKAPAELVEKNVDQSVLQQEFLEHAVNELYTRAVQEKQLRPVAQPQVSIAKFVPFS
                     TLEITAEVEAVGDITLGDYKKVKLARPVVTVTDKDVNEVLKNLQLRAATREEVKRAGA
                     EGDEVWIDFKGVDAKTKEPVAGADGKDYPLLLGSDTFIPGFEDNLTGVKAGGNKEFTL
                     TFPKDYGVKTLQNRKVTFNVTVNKVQKVAEPKLDDKFAAGVGPFKTLQELKTDVKAQL
                     EQQRRSDAERTYESQLLEKVAEKSHAAIPEVLVEEEVERLEQEERQNVAYRGQTWQEH
                     LDAEGVTAEEHRTKNRPAAELRVKAGMVLGEIARRENIEVTSEELELRLQLLKGQYQD
                     AAMQAQLDKPENVRDIASRLLTEKTLDILKSYAAQNA"
BASE COUNT          350 a          623 c          556 g          513 t
ORIGIN      
        1 cgagcagttt cttgatacgc aaactctcgc gcaggtcgat ctcctggtcg gtcaccttgc
       61 cgcgcgtgcc gctgctgggc tgatggatca tcacggtggc gtgcggcagt atgaagcgct
      121 tgcctttggt gccgctggag agcaagaagg ccgcggcgct ggcctgtacg ccgataccga
      181 ccgtctgcac gtcattggtt acgtactgga tggtatcgta gatagccagc gcatcataaa
      241 cgctgccgcc cggactgttg atatagaagt aaatgtcctt cttgtcgtct tccgcctgca
      301 ggaacaaaag ctgggcgacg acaaggttgg cggtcgcttc gttgacgtcg ctgcccagaa
      361 aaacgatgcg gtcctttagc agccgcgaat atatgtcgta ggcccgctcg taacggccct
      421 cgttctctat gacggtcggc acaagaatct gagccgtagg tttcccggaa taatcattca
      481 taacagatag tatacgcccg cttttagcac tcgtcaaccc agagtgccaa ctcacatccc
      541 ctgcttgttt tcggggtccg ccatcaaccc caaaacgact gcccgctgca tgggtatcag
      601 gccgtgcttg agcgccaggt ccggacggcg cgcgatcagg tcttcgtgag cttcctggtc
      661 atcggggcag ctgtccagcg ccgcctgttc cggagcctta taaagccatt caagcattct
      721 gcgcggcata gctctttaat atgtccaggg ttttttcggt cagcagccgc gaagctatat
      781 cgcgcacgtt ttcgggcttg tcgagctggg cctgcatggc cgcgtcctgg tactggccct
      841 tcagcagctg caggcgtaac tccagctctt cggaagtgac ttcgatgttc tcgcgccggg
      901 cgatttcgcc cagcaccata ccggctttga cgcgcagctc agccgcgggg cggttcttgg
      961 tacgatgttc ctcggccgtg acaccctcgg cgtccaggtg ttcctgccaa gtttggccac
     1021 ggtaggcgac gttctgtcgt tcctcctgct ccagacgctc gacttcctcc tccaccagca
     1081 cttccggtat ggccgcgtgg ctcttttcgg cgactttctc gagtaattgg ctctcgtacg
     1141 tccgttcggc gtcagaacgg cgctgttgtt cgagctgggc cttcacatct gttttcagct
     1201 cctgcagtgt cttgaacggc ccgacgccgg cggcaaactt gtcgtccagc ttcggctccg
     1261 ctactttctg caccttgtta accgtaacgt taaacgtgac tttgcggttc tgcagtgttt
     1321 ttacaccgta gtctttcggg aacgtaaggg tgaactcctt attcccgccg gccttcacac
     1381 ccgtcaagtt gtcttcaaag ccggggatga aggtgtcgct gcccagcaat aacgggtaat
     1441 ctttgccgtc ggcgccggct accggctctt tggtcttggc gtccacgcct ttaaaatcga
     1501 tccacacttc gtcgccctcg gcgcccgccc gcttgacttc ttcgcgggtg gcggcccgca
     1561 gttgcaggtt ctttagcact tcgttcacat ccttatcggt caccgtcacg acgggccggg
     1621 ccagcttcac cttcttgtag tcaccgagcg tgatatcacc cacggcttct acttcggcgg
     1681 tgatttccag cgtcgagaac ggtacgaact tggctatgga cacctgcggc tgagccaccg
     1741 gtcgcaactg cttttcctga accgccctgg tgtacagttc gttgacggca tgttccagga
     1801 attcctgctg cagcacgctc tggtccacgt tcttttctac cagttccgca ggtgccttgc
     1861 cggcccggaa gcccggcagt ttgacctgct tggcaaggtc gccaagcaca tgccccttca
     1921 cctgcagcag cagttcgctg tcggcggtaa tggtcaattt gaacttagtc ggattcagtt
     1981 tctccaaagc cacttgcata agaagctagt ttaacagatt agcagtggtt ttgccagtcg
     2041 ct
//
LOCUS       DASSFD010000111         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1414882, whole genome shotgun sequence.
ACCESSION   DASSFD010000111 DASSFD010000000
VERSION     DASSFD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414882"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFH39_02550"
     CDS             <1..677
                     /locus_tag="VFH39_02550"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HET6924683.1"
                     /translation="DPRDGFSAVIFQRLAESAIAAIQQRGRLPVLVGGTSLYVDSIIH
                     RYTFLAPPPPELREKLNAMSLPEVLELARSKGLDVSAVDIRNKRRVIRLIETDGAQPG
                     KEDLRPNTLILGIRTPRETLAARIAQRVDNMVAAGLELEVRQLAKKYGWDVEPMKGVG
                     YKEWREYFEGSASLNEVKQRIIKNTLDLAKRQRTWFKRNYSIQWISTPVDIISTVEEV
                     TTYLNK"
     gene            715..1302
                     /locus_tag="VFH39_02555"
     CDS             715..1302
                     /locus_tag="VFH39_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET6924684.1"
                     /translation="MVEQLFGSKTRVKLLQLFYSNPNRSFYVREITRKIDEQINSVRR
                     ELANLLNIGIITSDNTDNKLYYEVNQKYEFYEPLQQIFGGGKRRTKKTVAADVVDETD
                     ELKSLGHVELLVYTGQFTRDESTGVDVLVVGDVNRNAVAKYIAELEAKEGKELRYTVM
                     TPSDFQYRQTIKDRFVTQLSSAKKQILVDQSNLLD"
     gene            1322..1945
                     /locus_tag="VFH39_02560"
     CDS             1322..1945
                     /locus_tag="VFH39_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6924685.1"
                     /translation="MDLQFYGANCISITYKGTRLVVDDNLTDLGGKSILKPDDVALYT
                     MAHGEPKACKIAIDQPGEYEVSDISIMGIVARGHMDEEGQRTATMYKIVVGDFSVLVT
                     GHVHPDLPESKWEQVGIVDVMFVPVGGSGYTLDPLGALKLIKEIEPKLIIPTHYNVPG
                     LKYPVPQLDLANALKELAMEPKETVPKLKLKPADLTDLTQLIVLEKS"
     gene            complement(2128..2328)
                     /gene="rpmB"
                     /locus_tag="VFH39_02565"
     CDS             complement(2128..2328)
                     /gene="rpmB"
                     /locus_tag="VFH39_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET6924686.1"
                     /translation="MQKCDLTGKGKQYGNNVSFSQRHTKKVWKPNLQTKTVVVDGKKV
                     RMQLSTQAIRTLKKKGLLEPAK"
     gene            2408..3028
                     /locus_tag="VFH39_02570"
     CDS             2408..3028
                     /locus_tag="VFH39_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6924687.1"
                     /translation="MFGHLSGLEIAFVLITVIVSIGLHEAMHGFTAFKLGDRTAADLG
                     RITLNPLAHIDLFTTVLLPLLLISTGLPPFFAAKPVPFNPARVKWDEYGAALVGVSGP
                     LTNLLLAVLGAGLFHLVPYGLADDFFGIFVSINVALFVFNMIPFPPLDGSRLLYAFAP
                     EGLQNIMLRIEQLGLIAILILMILFFRVLGPVIISIDQNIINLLLG"
     gene            3281..3357
                     /locus_tag="VFH39_02575"
     tRNA            3281..3357
                     /locus_tag="VFH39_02575"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3315..3317,aa:Pro,seq:ggg)
     gene            3385..3894
                     /locus_tag="VFH39_02580"
     CDS             3385..3894
                     /locus_tag="VFH39_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924688.1"
                     /translation="MEEETQKKSRGARKRVKRVALSVVGLLVLLVAAGVAYTWYTGRQ
                     GPAVTAVVPAVQNTPPEPIRPSVPSAATQESAAVEFLSTPVPPGSDASINIKTVATSV
                     CSISVMYDTQPGTDPGLKPKTADDFGNVSWDWKVPANAPIGTWPVKVTCVRGAKSAVV
                     IGDLQVARQ"
     gene            3900..>4239
                     /locus_tag="VFH39_02585"
     CDS             3900..>4239
                     /locus_tag="VFH39_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924689.1"
                     /translation="MEEAVRHSPPSRAARKVSRLALAVVLGALLAVAGIYAYRHYAGL
                     PGPVPKSIRSALAFPVYYPEQRKLPAGYRLDSGSFRLAEEGVLLFSVSSSHGKQLIFS
                     EETSPGQEVIN"
BASE COUNT          983 a         1071 c         1212 g          973 t
ORIGIN      
        1 cagatccgcg ggacggcttc agcgcggtta tattccagcg attggccgaa agcgccattg
       61 cggctattca gcagcggggg aggttacccg tactggtcgg cggcaccagt ttatatgtag
      121 acagtattat tcaccgctac actttcctgg caccgccccc gcccgagttg cgggaaaagt
      181 taaacgccat gagtctgcct gaggtgctcg agctggcaag gtccaagggg ctggatgtgt
      241 ccgccgtaga tatccgcaac aagcgtcggg taataaggct gatagagact gacggcgctc
      301 agccaggcaa ggaggactta cgaccaaata cgctcatact tggcatccgg acgccgcgcg
      361 agacccttgc ggcacggatt gcgcagcggg tcgataacat ggtagcggcc ggactggaac
      421 tggaggtgcg gcagctggcc aaaaaatacg gttgggatgt agagccgatg aagggcgtgg
      481 gctacaagga gtggcgcgag tactttgaag gttcggccag cttgaacgaa gtcaagcagc
      541 ggatcattaa gaacacgctt gacctggcca agcgccagcg cacttggttc aagcgcaact
      601 atagtattca gtggatttct acccctgtag acatcataag tactgtagaa gaagttacaa
      661 catacctgaa caaataacat tcaattgctc acggctgttt gctacaatag tcgtatggtc
      721 gaacaattgt ttggttccaa aacccgcgtc aagttgctgc agctgtttta tagcaatccg
      781 aaccggtcat tttatgtgcg cgaaataacg cgcaagatag atgagcagat caactcggtg
      841 cgccgcgagc ttgccaattt actgaacatc ggcatcatta cttcggacaa caccgacaac
      901 aaactgtact acgaagtgaa tcagaaatac gagttctacg aaccgctgca acagatattc
      961 ggcggcggta agcgccggac caaaaagacg gttgcggccg atgtggtgga tgagaccgat
     1021 gaactcaaga gcctgggcca cgtagagctc ctggtatata ccgggcagtt cacgcgtgat
     1081 gaatcgacgg gggtagacgt gctagtggtc ggcgacgtga atcgcaacgc cgtggccaaa
     1141 tacatagccg agctggaggc taaagagggc aaggagttgc gctatactgt tatgacgccc
     1201 agtgactttc agtaccggca aactattaaa gaccggttcg tcacccagtt atcatccgcc
     1261 aagaaacaga ttctggttga ccagagcaac ctgttagact aataaaaagg agtgagaacc
     1321 tatggatctt cagttctatg gagctaattg catcagcatc acgtacaaag ggacccggct
     1381 ggtggtggac gataacctga ccgacctggg aggtaagagt atcctaaagc ccgatgatgt
     1441 ggcgctgtac accatggcgc atggcgagcc caaagcctgc aaaattgcca tagaccagcc
     1501 gggcgagtat gaggtaagcg atatatccat catgggtatt gtcgcgcgcg gccatatgga
     1561 tgaggaaggg caacgcacgg ccaccatgta taagatcgta gtcggcgatt tcagcgtgct
     1621 tgttaccggg catgtccacc cggatttgcc ggaaagcaaa tgggagcagg tgggtattgt
     1681 ggatgtcatg ttcgtgccgg ttggcggcag cggctacacg ctggacccgt tgggcgccct
     1741 gaaacttata aaagagattg agccgaagct gattataccc acgcattaca atgtgccggg
     1801 actgaaatat ccggtgccgc agctggattt ggccaatgca ctcaaggaac tggccatgga
     1861 gccgaaggag actgtgccaa agctgaaact caagcctgcc gatctgacgg acctgacaca
     1921 gctcatcgta ctcgaaaaat cttgattgag ccatcaccca gaaaagctgc gtctatgtcg
     1981 agcggctagt gtattttacc gtccatgcct gccgctccta atgtacgtac tctggctgct
     2041 gtttggaggc ctgatgtttt gggcctgctc aaatgttaga agagcctcca ctttctggag
     2101 gctctttctt ccggtgacgt tcagctgtta tttggcaggc tcaagcagac ccttcttttt
     2161 cagggtgcgg atggcttgag tggaaagctg catacggacc tttttgccgt ctacaaccac
     2221 ggtcttggtc tgtaagttgg gcttccagac tttcttggtg tggcgctggg aaaagctgac
     2281 gttgttaccg tactgcttgc ccttgccggt cagatcacac ttttgcataa cccgtagatt
     2341 gtaccctata acacctttcg gtgcaacccc tgtattgtta tgatagttat ggctatactt
     2401 aagggatatg tttggtcatt tatccggact agagattgcg ttcgtactta taacggtcat
     2461 cgtttcaatc ggattgcacg aagccatgca cggctttacg gcctttaagc tgggcgatcg
     2521 gacggctgca gacctgggga gaattacgct gaacccgctg gcgcatatag acttatttac
     2581 aactgtacta ctacctttac tccttatatc caccggttta ccgccctttt ttgctgccaa
     2641 gccggtgcca ttcaacccgg cgcgcgtaaa gtgggacgag tacggtgcgg cgctggtcgg
     2701 cgtgtccggc ccgctcacga atctccttct ggcggtactg ggagcgggac tgttccattt
     2761 agtaccgtac gggctggccg atgacttctt cggtatcttc gtaagcatca acgtcgcgct
     2821 gttcgtgttc aacatgattc cattcccgcc gctggacggt tcacgcctgc tgtacgcttt
     2881 tgcgccggaa gggctgcaga atataatgct gcgcattgag cagctggggc ttatagccat
     2941 actcatcctg atgatactgt tcttcagggt attgggcccg gtaatcatta gcatagacca
     3001 gaatataatc aatcttttgc tgggctagga gcacgggtat aatagaggta gtcgggtagg
     3061 gaattcgccc agaacgaatg ctccccgatg ccgagcgaat gattatctga atactaatca
     3121 ttagggagct caatatccgc cggaaccgtg gttctggcat gcgagcaccc acctgggatg
     3181 ccatccaggt gatcacctcg gtacccgact ttaagtaaaa ggctcaggtt ttctgggctt
     3241 tttactttaa cggcagtgtt ataattcaat agcctaacaa cggggtgtgg cgcagttggc
     3301 tagcgcgtac ggctgggggc cgtgaggtcg caggttcaag tcctgtcacc ccgaccataa
     3361 tcttatgctg ataagatgga tgtaatggaa gaagagacac aaaaaaagag taggggggca
     3421 cgaaagcggg tgaagcgggt tgccttaagc gtcgtggggc tgctggtact gcttgtagca
     3481 gctggcgttg cctatacctg gtatacgggc cgtcaggggc cggccgtgac ggcagtagtg
     3541 cccgcggtac agaacacccc gccggagcct atcaggccat cggtgcctag tgctgctacg
     3601 caggagagcg cggcggttga gtttctgagt acacccgtcc cgccgggaag cgacgcctcc
     3661 attaacatca agactgtggc cacgtccgtc tgcagcatat ccgttatgta cgacacgcag
     3721 ccgggtactg acccgggcct gaagcctaag acggccgacg acttcggtaa tgtcagttgg
     3781 gactggaaag tgccggcgaa cgcgcctatc ggtacctggc cggtgaaagt tacctgcgtg
     3841 cgcggcgcca agagcgcggt cgtcatcggc gatctgcaag tagcgaggca ataatacgag
     3901 tggaagaagc agttagacac tcgccgccct cccgggctgc acgcaaggtt tctaggctgg
     3961 cgttggcagt ggtgctgggt gcgctcctgg ctgttgccgg gatctatgcc tatcgccact
     4021 atgccggact gcccggtccg gtacccaagt ctatacgttc tgcgctagca tttccggttt
     4081 attaccccga gcagcgaaaa ttacctgcgg gctatcggct ggattcggga tcgttccgtc
     4141 tggcagaaga aggagtccta ctgttttccg tgtcgtccag ccacggcaaa cagctgattt
     4201 tttccgagga aacctcaccc ggccaggaag ttatcaata
//
LOCUS       DASSFD010000112         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1482711, whole genome shotgun sequence.
ACCESSION   DASSFD010000112 DASSFD010000000
VERSION     DASSFD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482711"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFH39_02590"
     CDS             <1..209
                     /locus_tag="VFH39_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924690.1"
                     /translation="GCGGIIAMLGEQNVLDMDPKDVFGAIFGREAEDKYGSLHFKCRY
                     GHSNRRPRNQLLEKCQVCKVSVRC"
     gene            608..1201
                     /locus_tag="VFH39_02595"
     CDS             608..1201
                     /locus_tag="VFH39_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HET6924691.1"
                     /translation="MKRIGIYAGTFDPVHAGHVGFALQAMDKANLDTVYFLPERRPRH
                     KQGIEHFGHRVAMLKRAARPHRNFEVLELEDVSFTMERTLPKLQRRFRGQQLVFLFGS
                     DTAMQLPAWPNVENMLMTSELVVAARAGSALEHIRQDITGWPVKPLRLHLFESFAPDI
                     SSGKVREALRRRTYVRGLLASVARYANRNWLYISLNQ"
     gene            complement(1525..2262)
                     /locus_tag="VFH39_02600"
     CDS             complement(1525..2262)
                     /locus_tag="VFH39_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6924692.1"
                     /translation="MSKQFWAVIVVILVVLGGIFAFTGSKSGNSNSGGSKASPTNHIE
                     GQGKSGVKLVEYGDYECPVCGIYYPVVKQVAQQYDQQIYFQFRNFPLTSIHPNAFAGA
                     RAAEAASLQGKFWEMHDKLYVNQDPNGHSGWVASSDPLNQYFVDFAGQIGLDINKFKT
                     DYASDQVNNAVNADLSKANSLGLNGTPTFFLDGKQLDNSVFTDSNGPSLAKFQQVLNA
                     EIAKKGGTPPGGSGNTPGQSPAATNNQ"
BASE COUNT          514 a          617 c          659 g          515 t
ORIGIN      
        1 tcggttgcgg cggcataatc gctatgctcg gcgaacaaaa tgtcttggac atggacccca
       61 aagacgtctt cggcgcaatc ttcggcaggg aagcggaaga caagtacggt tcgctgcact
      121 tcaaatgcag atacggtcac agcaaccgcc ggccgcggaa tcagctgctg gaaaaatgtc
      181 aggtttgcaa ggtcagtgtc cgctgctaga cgccgtggca gcgtataatt agtgtatgct
      241 ggaagaccaa aatatagcgg ccgaagagtt cgatgcctat gccggactag tgaaggaaaa
      301 atcaccgtat ttattctgtg atgcctgcgg gttttatcct atagaaccag tgcatggcca
      361 cttcgtctgc cccagctgca agatgcccac gaaatgctgc gaaggcgccc cggcagaatg
      421 accgccggcc tacccattgt gaaaggactg tcctctcaca aagagttgag gcgttatatt
      481 ctatcggtaa aatttatact ttgtgagagg acagtccttt cacaatgaag cgacctggga
      541 tttacgccgg tactttcgac cccagaacat gccagcggca tgtttctgta gacgcgaggg
      601 aaccgtcgtg aaaagaatag gcatttacgc cggtactttc gacccggtgc atgccgggca
      661 tgtcggcttc gctttgcagg cgatggacaa ggccaatctg gatacagtgt attttttgcc
      721 cgagcgccga ccacggcaca agcaggggat cgagcatttc ggccaccgcg tagcaatgct
      781 gaagcgggcc gcccggccgc accggaactt cgaggtactg gaactggaag acgtgagctt
      841 caccatggaa cgcacgctac cgaaattgca gcggcgtttc cgcggccagc agctcgtgtt
      901 tctgttcgga tccgacaccg ccatgcagtt gcctgcctgg ccgaacgttg aaaatatgct
      961 tatgactagc gagctggtgg tggcggcccg cgccggctcg gccttagaac atatccgtca
     1021 ggacatcacc ggctggccgg tgaagccgct gcggctgcac ttgttcgaaa gttttgcacc
     1081 cgatatcagc agcggcaagg tgcgcgaggc actacgccgc cgtacatatg tccgcggcct
     1141 gctggcaagt gtcgcccgct acgcgaaccg caactggctg tacatatcgc tcaatcagta
     1201 agaaaagatt gttgacaagc atgagcgctt tgatacaatg gcggagtatt acataggcgg
     1261 aatgcgagag ccgccgcgtg agacaaaaac attaaaaaat aaaaaataga gccgggcgat
     1321 gcttatggca gggcattcgt gacggtggag aggtatgtta tgccggtaaa aataaaaata
     1381 gtcaacagca agcccgaaaa ggtagcagtg cgggtccgcc gcccgtcctg gcagcagttt
     1441 atacactatt aggccttagc ggcctgcaga agcctcacac caggagctcc agaatacggg
     1501 gctcctctct tatttatgcc tagtctattg gttgttggta gcggcggggc tttgaccggg
     1561 cgtgttgccg ctgccgcccg gcggcgtgcc gccctttttg gcaatctcgg cgttcagtac
     1621 ctgctggaac ttagcgaggc tggggccgtt actgtcggtg aaaaccgagt tatccagctg
     1681 ttttccatcc aggaagaatg tcggcgtgcc gttcaggccg agcgaattgg ccttggacag
     1741 gtcggcgtta acggcgttgt ttacctgatc gctggcgtag tctgtcttaa acttattaat
     1801 atccagccct atctgcccgg cgaaatccac gaagtattgg ttcagcgggt cgctgctggc
     1861 tacccagcca ctgtggccgt tcgggtcctg gttgacgtac agtttgtcat gcatctccca
     1921 gaacttgccc tgcagactgg cggcctcggc cgcccgggcg ccggcgaagg cgttggggtg
     1981 gatggaagtc agcgggaagt tacggaactg gaagtagatc tgctggtcgt attgctgggc
     2041 cacctgcttc acgactgggt agtaaatgcc gcacaccgga cactcgtagt cgccgtattc
     2101 caccagtttc acgccgctct taccctgtcc ctcgatgtgg ttcgtaggcg acgctttgct
     2161 gccgccggaa ttcgaattgc cggacttact gccggtaaag gcaaagatac cgcccagcac
     2221 caccagtatg acgactatga cggcccaaaa ctgttttgac accttgtttt ctccttattc
     2281 agtcttaagt atacaccaca gtttt
//
LOCUS       DASSFD010000113         5610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1033895, whole genome shotgun sequence.
ACCESSION   DASSFD010000113 DASSFD010000000
VERSION     DASSFD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033895"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VFH39_02605"
     CDS             <1..1425
                     /locus_tag="VFH39_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6924693.1"
                     /translation="FKLRARFILVLVLLLLAMFAAITFVIVRENTHTLRNGLVNQSKS
                     FAALATQPIGSNFVLYSQSGTIRIHQKVDSFTALDPDINQVEIVDTMGNILFADQPDN
                     QIPVPASAASAISPTYLHDQNGNLSAIVQPYLEDFGIHRYAVVYSISYANVDKSVREI
                     VTLILSLSIAILLVSLVVWYGLINRLFLRPVARISKDTLRISQGDLEHQVRVGRNDEI
                     GDLAAAVNKMAASLLDDIAKLKEVDRLKSEFLMITSHNLRTPLTVIDGYLSEIQGADS
                     LEELQQLVQPIAASVKRLQGFAEDVLTISTVEAGHDMVDRQPMALQPVLEQVASDFEP
                     LAREKKLQFVAHVATDAWAAIDRIHFHNALWNLLDNAYKFTPEGGRITLDVRSAGTQV
                     EVVVSDSGIGIAAEELPKLFTKFHRGTDTLTYNYEGTGIGLYITRLLMRQLGGDAKVE
                     SEAGRGSTFTLSLPTVEPAAKSAA"
     gene            complement(1437..4286)
                     /locus_tag="VFH39_02610"
     CDS             complement(1437..4286)
                     /locus_tag="VFH39_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6924694.1"
                     /translation="MAKRTATSDQLPKDRLLPRLAVHLFGLPKLTILLWLVLVVFGAL
                     SYTVLLRREGFPSINIPIAIVQGTYIVNDPSQVDQRVAKPITDLALRQSGVNTVRTTS
                     SANFYSIVVQYQQSANARQATAQLEQAVHRQLKLPPGATAAFNVPYFGVTGGDTQKID
                     DLISLYKLQGSATTAQLAGKAQQAVDYLNAHKPPLVKTFFVRDPFKTATDPRTGRQVT
                     VQQSFDRFGQRQDGTNRFYNSVAIGVTGVNNVDVIKLDAQLKPLLAQLTAQPTFHGYA
                     ATISASSAPSINQEISELQRVLLEGLIAVLVVGSIVIAVRASIITVLSMITVILTTLG
                     LLYLIGYTLNVITLFALILGLSLVVDDTIIMTEAIEASRRHETTAKAAIALATRKIAR
                     AMVAATLTAVLSFAPLLFVSGILGGFIRQIPVTLIAALLISLLVALIFIPLFARFLLL
                     TKGQLARTRRKASRAAGLEAGIARLICRPMLWARGSTPKLFTVGIIAVIISLLFVGAA
                     GAIFKKVTFNIFPPTKDTNALSVTLDFPPGTTIPKAEDIAAQADKLVAKTLGPNFQSA
                     SYYGTGSDTQAMIEINIIPYQQRSVTSQQLVKQLQSRFDRDFTAANAQVGQVDVGPPT
                     SGFTVQIHTEDRAKGYRLAADVADYLRHTTLTRLSGTTAGFKNVTVSSPNQVVRKDGV
                     LTIDVTANFTADDTSTLVTLAQNAVKQHYSARKLASFGLPQNTISFDIGQESDNQNSF
                     KSLALAFPLLLLVIYILLAVEFRSLLQPLLIFMAIPFSLFGVTLGLYLTDNAFSFFTM
                     MGFFALIGLSLKNTILLTDYANQSRRRGLGAIDSAVEALGERFRPLIATSLTAVVSLI
                     PLAVTSPFWQGLAIVLIGGLLASTTLVILVFPYYYLGGEYLRTHFSRRGAIAWLVATL
                     VLVIIAAKLEARLVWLAVAISLLGALVWHRLRRHR"
     gene            complement(4304..4708)
                     /locus_tag="VFH39_02615"
     CDS             complement(4304..4708)
                     /locus_tag="VFH39_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6924695.1"
                     /translation="MSNETATGKRILCIEDEHFIGELYTRALSKAGYEVVLEPDGERG
                     LALALTNEFDIILLDLMVPSLTGVEILRKLRDPNLPTRLKAKIIVATNLEQRDDVRAS
                     IEQQSDGYLVKAEITPHELVAFLDRLQTGNAA"
     gene            complement(4740..>5610)
                     /locus_tag="VFH39_02620"
     CDS             complement(4740..>5610)
                     /locus_tag="VFH39_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6924696.1"
                     /translation="LFDLAAYYTKDSPTGVETMLVLFDHTKAYSQDDQAISFIALTVH
                     ELRTPLTLLRGYIEAFEEELDGKLDPEMKDFMFKMSATAQQLIAFVNNILNVARLDDD
                     QLELQLREQPWGDIVRAAANGLALRAKVRGITLEYSIAQGLPTVGVDRMSIIEVINNL
                     VDNAIKYSGESKRIRIATRMNAEGLVETTVQDFGVGIPDNVIPNLFTKFYRDHHNRAQ
                     IGGTGLGLYLSKAIVDAHGGSIWVTSKVGEGSTFGFTLLPFSQLSSEQRTADNSIVRD
                     THGWIKNHSLYRR"
BASE COUNT         1073 a         1606 c         1828 g         1103 t
ORIGIN      
        1 tttaaactgc gggcccgctt catcttggtg ttggtgctgc tgctgctcgc catgtttgcg
       61 gcgatcacct tcgtaatcgt ccgcgagaat acccatacct tgcgcaacgg cctggtcaac
      121 cagtcaaaat cgttcgccgc cctggctacc cagccgatcg gcagcaactt cgtgctgtac
      181 agccagtccg ggaccatccg gatccaccag aaggtggaca gtttcactgc tctcgacccc
      241 gacataaacc aggtcgaaat cgtcgacacg atgggcaata tcctgtttgc cgaccagccg
      301 gacaaccaga taccggtgcc ggcctccgca gccagtgcca tctcgccgac ctacctgcac
      361 gatcagaacg gtaatctgtc ggctatcgtc caaccgtacc tcgaagactt cggtatccac
      421 cgctacgcgg tcgtctactc catttcctat gccaacgtgg acaagagcgt ccgcgagatc
      481 gtgacgctca tcctcagcct cagcatcgcc atcctactgg tgtccttggt agtgtggtac
      541 gggcttatca accggctgtt tctgcgcccg gtggcgcgga tcagcaaaga tacgctgcgg
      601 atcagccagg gcgatctgga acaccaggtg cgagtcggcc gcaatgacga gatcggtgat
      661 ttggcggcgg ccgtcaacaa gatggccgct tccctgctcg acgacatcgc caaactgaag
      721 gaggtggacc gcctgaagag cgagttcctg atgatcactt cgcacaacct gcgcacaccc
      781 ctgacggtca tagatggcta cctgagcgaa atccagggcg ccgactcgct ggaggagctg
      841 caacagctgg tccagccgat cgccgccagt gtcaaacgcc tgcagggttt cgccgaagac
      901 gtcctgacga tttcgaccgt cgaggccggt cacgatatgg tcgaccgcca gccgatggcc
      961 ttgcagccgg tccttgagca ggtggcaagc gatttcgagc cgctggcccg cgaaaagaag
     1021 ctgcagttcg tggcgcacgt cgccacggac gcctgggccg ccatcgaccg catccacttc
     1081 cataacgcgc tgtggaatct gctcgataac gcctataagt tcacgcccga aggcggccgg
     1141 atcaccctgg atgtccgctc ggcgggcacg caggtggagg tcgttgtcag cgacagcgga
     1201 atcggcatcg ccgccgagga gcttcccaag ctgttcacca agttccatcg gggcaccgac
     1261 acgttgacgt ataactacga aggcaccggc atcggcttat acattacccg gctgctgatg
     1321 cggcagttgg gaggcgacgc caaggtcgag agcgaggcgg gccgcggcag caccttcacg
     1381 ctcagcctgc cgacggtcga accggccgct aaatctgctg cctgataacc tggccgctag
     1441 cggtgtcggc ggagccggtg ccagaccaaa gccccgagca ggctgatggc caccgccagc
     1501 cagaccaacc gtgcctccag cttggccgcg atgatgacca gaacgagggt ggcgaccagc
     1561 caagctatgg caccgcggcg gctgaagtgc gtgcgcaagt attcgccgcc gaggtagtag
     1621 tacgggaata ccaggattac aagggtggta ctggccagca gaccgccgat gagcacgatg
     1681 gccagcccct gccagaacgg actggtcacg gccagcggga taagcgacac caccgccgtc
     1741 aggctggtgg cgataagcgg ccggaaccgc tcgccgagcg cttcgacagc cgaatcgatg
     1801 gctcccaggc ccctgcgacg cgactggttg gcataatcgg tcaacaaaat ggtgttcttg
     1861 aggctcagac cgatcaacgc gaagaagccc atcatggtga agaagctgaa agcattgtcg
     1921 gtcaggtaca ggccgagcgt caccccgaac aggctgaacg ggattgccat gaagatgagc
     1981 agcggctgca atagcgagcg gaattcgacc gccagcagga tgtatatgac cagcagcaac
     2041 agcgggaatg ccagtgccag cgatttgaac gaattctggt tgtcggactc ctggccgata
     2101 tcgaacgaga tagtgttctg cggcagaccg aagctggcca acttgcgggc cgagtaatgc
     2161 tgtttgaccg cgttttgagc cagtgtgacg agcgtgctgg tgtcgtcagc ggtgaagttg
     2221 gccgtgacgt cgatggtcag gacgccatcc ttgcggacca cttggttggg gctgctgacg
     2281 gtgacgttct tgaagccggc ggtggtaccg ctcagccgtg tcagggtggt gtggcgcagg
     2341 tagtcggcga cgtcggcggc caggcgatag cccttcgccc ggtcttcggt atggatctgc
     2401 accgtgaaac cggaggtcgg cggtccgaca tccacctgcc ccacctgggc gttggccgcc
     2461 gtgaaatcgc ggtcgaagcg gctctgcagc tgtttgacga gttgctggga agtcacgctc
     2521 cgctgctgat acgggatgat gtttatctcg atcatggcct gggtgtcgct gcccgtgccg
     2581 tagtaggaag cgctctggaa gttggggccg agcgttttgg cgaccagctt gtcggcctgg
     2641 gcggcgatgt cctcggcctt gggaatggtc gtgcccggcg ggaagtcgag cgtcaccgac
     2701 agcgcgttgg tgtctttggt gggaggaaag atattgaacg tcaccttttt gaaaatggcg
     2761 ccggcggcgc cgacgaacaa caggctgatg atgacggcga tgatgccgac ggtgaacaat
     2821 ttgggcgtcg agccgcgggc ccacaacatc ggtcgacaga tgagacgcgc gataccggct
     2881 tccaggccgg cggcgcggct ggccttgcgc cgggtacggg cgagctggcc tttggtgagc
     2941 agcaggaagc gcgcgaacaa cggaatgaag atgagcgcca ccagcagact gatgagcagt
     3001 gccgcgatga gcgtgaccgg aatctgccgg atgaacccgc ccaggatgcc gctgacgaac
     3061 aacagcggtg cgaagctcag gaccgcggtc agggtggcgg cgaccatcgc ccgggctatc
     3121 ttgcgcgtcg ccagcgcgat ggcggccttg gcggtcgttt cgtggcgccg gctggcctcg
     3181 atggcttcgg tcatgatgat ggtgtcatcg accaccaggc tgaggcccag gatgagcgcg
     3241 aacagcgtga tgacattgag cgtatagccg atgaggtaca gcaggccgag cgtggtcagg
     3301 atgacggtga tcatggaaag caccgtgatg atactggcgc ggacggcgat gacgatcgag
     3361 ccgacgacca gaacggcaat cagcccttcc agcagtacgc gctgcagttc gctgatttcc
     3421 tggttgatac tgggcgcact gctggcgctg atagtggcgg cgtagccgtg aaacgtcggc
     3481 tgcgcggtca gctgagccag cagcggtttc agctgcgcat cgagcttgat gacatcgacg
     3541 ttattaacac cggtgacacc gatggccacg gagttgtaga accggttggt gccgtcttgc
     3601 cgctggccga aacggtcgaa ggactgctgg acggtcacct gccggccggt ccggggatcg
     3661 gtggccgtct tgaaggggtc gcggacgaag aatgtcttta ccaacggcgg tttgtgggcg
     3721 ttcagatagt cgactgcttg ctgggcctta cctgccagct gggcggtggt ggcactgccc
     3781 tgcagtttgt agagcgatat caggtcatcg atcttctggg tatcgccgcc cgtgacgccg
     3841 aagtacggca cattgaaggc cgcggtggcg ccgggcggta gtttgagctg ccggtggacc
     3901 gcctgctcaa gctgggcggt cgcctggcgg gcattggcgg attgctggta ctggacgacg
     3961 atggaataaa agttggcgga actggtggtg cgcacggtgt tcaccccgct ctgccgcagc
     4021 gccagatccg tgatgggctt ggcgaccctc tggtccacct gcgacgggtc gttgacgata
     4081 taggtaccct ggacgatggc gatcgggatg ttgatgctcg ggaaaccttc acgcctgagt
     4141 aggacggtgt agctgagcgc gccgaacacc accagcacca gccagaggag aatcgtcagc
     4201 ttcggcaggc cgaacaaatg gacggccagg cgtggcagca gccggtcttt cggcagttgg
     4261 tcagaagtgg ctgtccgctt cgccataccg tatcccccag gtgttaggca gcgtttccgg
     4321 tttgcaggcg atccaggaaa gcgaccagtt catggggcgt tatctcggcc ttcacgaggt
     4381 agccgtcgga ctgctgctcg atgctggcgc ggacgtcgtc acgctgttcc agattggtgg
     4441 cgacgattat cttggccttg agtcgggtcg gcagattggg atctcgcagt tttcgtaaga
     4501 tttcgacgcc ggtcagcgac ggtaccatca aatcgaggag gatgatatcg aattcattgg
     4561 tcagcgccag cgccagacca cgctcgccgt ccggttccag gactacttcg tagcccgctt
     4621 tggaaagagc ccgggtgtag agttcgccga tgaaatgctc atcttcgatg cacaggattc
     4681 tcttgccggt tgccgtttcg ttagacatag gcatattgta gcagaggacg ggtgattcgt
     4741 cagcgacggt acagcgagtg gtttttgatc cagccgtgag tgtcacggac gatactgttg
     4801 tcggcggtgc gttgctcgct gctgagctga ctgaacggga gcaaggtgaa gccgaacgta
     4861 ctcccctcac ctaccttact tgtcacccag atactgccgc cgtgggcgtc gacgattgct
     4921 ttactgaggt acaagcccag tcccgtaccg ccgatctgcg cgcgattgtg gtggtcgcgg
     4981 taaaatttgg tgaacaggtt gggaatgaca ttatccggga taccgacacc gaagtcctgg
     5041 acggtcgtct cgaccagccc ttcggcattc atgcgggtag cgatgcggat gcgtttgctc
     5101 tcgccgctgt acttgatggc gttgtcgacc aggttgttga tgacttcgat gatgctcatg
     5161 cggtcgacgc ccaccgtcgg cagcccctga gcgatgctgt actccagcgt tatgccgcgg
     5221 accttggcgc gcagcgccaa gccgttggcg gctgcccgga cgatgtcgcc ccacggctgt
     5281 tcacggagct gcagctccag ctggtcatcg tccaggcggg cgacgttgag gatgttgttg
     5341 acgaaggcga tcagctgctg ggcagtggcg ctcatcttga acatgaaatc tttcatttcg
     5401 gggtcgagct tgccgtccaa ctcttcctcg aaggcctcga tgtagccgcg cagcaacgtc
     5461 agcggtgtcc tgagttcgtg gaccgtcagg gcgatgaagc tgatcgcctg atcgtcctga
     5521 ctgtaggcct tggtatggtc gaacagcacc agcatggttt cgacgccggt cgggctgtcc
     5581 ttggtgtagt aggcggccag gtcgaacagt
//
LOCUS       DASSFD010000114         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1474241, whole genome shotgun sequence.
ACCESSION   DASSFD010000114 DASSFD010000000
VERSION     DASSFD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474241"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1843)
                     /locus_tag="VFH39_02625"
     CDS             complement(227..1843)
                     /locus_tag="VFH39_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924697.1"
                     /translation="MGIPTWHMERQWQNNRMDPNSRIFDAVPLARTLLVLPPVALRSV
                     TSKDGTSVYASSDTLFWGAVFGRDSLEVAEDLIRIKPGLVHNILLTLGRLQGVTHNAA
                     NEEDPGKIIHESRTVRINGEYIKGEPLRIFRTLAPKWGGNDEALAYYGSVDSTPHFLR
                     TLGMYCLAYGDNILSETVVRRDGETGTMREAAEAAAGWLNDRLAASRSGLIEFERMNP
                     LGIANQAWKDSDEFYVHENGEFANHAAPIASIEVQALAYDGLLAAARLLPDRARDYAA
                     KAHRLRNRTLQLLWQPGRQYFALGIDFDRSGAQRIIKTRTANPGALLDSGLLEGLPAP
                     IQKKYVAGIVQTIMSADFLTDAGIRSRARDQAHLVDHADYHGSLVTWPKETYDIAKGL
                     RRWGFQKLARQLEDRLLNLVLRFHEYPEFVYVDAHGRVFGGRPVRRKHGEINLASNIT
                     PIAGRNIPERIQAWTVSAIMAIVTERLSNKLRPPFSRQQRVPLPWITHLEGEILATIP
                     QRRRSLSPLKLALGYPNYKYAVAPRDKTAL"
     gene            complement(2334..>2614)
                     /locus_tag="VFH39_02630"
     CDS             complement(2334..>2614)
                     /locus_tag="VFH39_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6924698.1"
                     /translation="VKTLPGQLVLEAGQGTAVNIYEKEDHEPWDSTVLGIETEDVDTA
                     VKQLLDAGIETAKLPGTDENGIMRDPDIGNAAWFQDPAGNWICISNIV"
BASE COUNT          511 a          765 c          740 g          598 t
ORIGIN      
        1 tcgcaatcca ccgtggaatc ggccggcccc agcaggacgg gttcgtgccc cgcctcttga
       61 aggcctttga tcaggcaata gatgacttgt tcagcgccgc cgtaccgccc cggcggtatt
      121 ggtatgtagg gaccggccac aatagcgact ttcatctgtt cccttggcta cccctcttga
      181 caggtactac tataggccgc cgccgccgtt acggctgtaa ggattcttat agagcggtct
      241 tgtcccgggg cgccacagca tacttgtaat tcgggtaccc aagagcgagc ttaagggggc
      301 tgagtgagcg ccgccgctgc gggatagtcg ccagaatttc cccctccaga tgcgttatcc
      361 agggaagggg aacgcgttgc tgcctcgaaa agggcggccg tagcttattc gacaggcgtt
      421 cggtcacgat agccattatg gcggaaacgg tccaagcctg gatgcgctcc ggaatgttcc
      481 tgccggcaat aggcgttatg ttgctggcga ggttgatttc gccatgcttg cgccggaccg
      541 gccgccctcc gaatacgcgt ccatgggcat ctacatagac gaactcggga tactcatgga
      601 acctgagaac gagattgagc agccggtcct ccagttgacg tgccagcttt tgaaatcccc
      661 agcgcctgag gcctttggcg atgtcgtatg tttccttcgg ccaggtaaca agggagccgt
      721 gataatcggc gtggtccacg aggtgggcct gatcgcgcgc ccggctgcgt atgccggcat
      781 cggtcagaaa gtccgcactc ataatggttt ggacaatacc ggccacatat tttttttgaa
      841 ttggcgccgg taaaccctcc aacaacccac tgtccagcag cgcgcccggg ttggcagtcc
      901 tggtcttgat aatacgctgg gcgcccgacc ggtcgaagtc gatccccagc gcgaaatact
      961 gccggccggg ctgccatagc aattgcaggg tgcggttacg caaccgatgg gccttagcgg
     1021 catagtcgcg ggcacgatcc ggcaaaagcc gggccgccgc caacagcccg tcgtacgcca
     1081 atgcctgcac ttctatggaa gctattggcg ctgcatggtt ggcgaactca ccgttttcgt
     1141 gcacgtaaaa ttcatcggaa tctttccagg cctggttggc tatgccgagc ggattcatcc
     1201 gctcgaattc gatgaggccg gaccttgagg cggcaagccg gtcgttcagc cagccggccg
     1261 ccgcctcggc cgcttcgcgc atcgttccgg tttcgccgtc ccgccgtacc actgtctcgc
     1321 tcaggatatt gtcgccgtat gccagacagt acatgccgag ggtacgcagg aaatgcggag
     1381 tggaatccac ggagccgtaa tacgccagcg cttcatcgtt gccgccccac ttgggtgcta
     1441 gggtgcggaa tatgcgcaaa ggttcaccct tgatatattc gccgttgatc cggacggtgc
     1501 gtgattcatg gatgattttg ccgggatctt cctcgttcgc cgcattgtgt gttacgccct
     1561 gcaggcggcc gagtgtcagc aatatgttat ggaccagccc gggctttatg cggatcaggt
     1621 cttcggcgac ttccaaagag tccctgccga aaaccgcccc ccagaacaga gtgtccgagc
     1681 tggcatacac gcttgtaccg tctttggagg tgacgctcct cagggctacc ggaggcagaa
     1741 ccaataaggt gcgggcaaga ggtacggcat cgaagatacg gctgttcggg tccatgcgat
     1801 tattctgcca ttgccgctcc atatgccatg taggaattcc cacatacagt atagcgctgc
     1861 ccggcttttt tgcctggtat gcttagcgcc gccgaatgcg ctggcttagc agcaacgaca
     1921 tggcgcccac catcagcagt gccgcggcgc actccatgaa gaacaggccg agcccgatag
     1981 ggttgttcca gtcaaagatc atagctgctc cttctccgaa gagcttagtt gctgtaacac
     2041 tttatgatga tgccttgtaa tcccgcatgc tgcaattata tttctaacac tagatccttg
     2101 gttcccgggg gtaagacgga cggcgatgct aagcccctct atccgttccg tttcggcaac
     2161 ggtacggcat tatcgataaa gccgcggcca ttttacccac cggtcgcagc tgtaatgccc
     2221 ccgatgcggg tgtctcatgg aatggggatg ccagcgccgg caccgccggg gccctgcatt
     2281 ggcagtgtgc cggcaacgaa gttagagcta tccgactttt gggcggcaca gccctacaca
     2341 atattactta tacatatcca gtttccggca ggatcttgga accaggctgc atttccgatg
     2401 tccggatcgc gcatgattcc gttttcgtcc gtgcccggta gtttcgcggt ctcaatgccg
     2461 gcatccagca attgtttcac ggccgtgtcg acatcttcgg tttctatacc cagaacagtt
     2521 gaatcccacg gttcgtggtc ttccttctcg taaatattaa ccgctgttcc ttgtccagcc
     2581 tcaagtacca gttggcccgg caatgttttt acga
//
LOCUS       DASSFD010000115         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_27277, whole genome shotgun sequence.
ACCESSION   DASSFD010000115 DASSFD010000000
VERSION     DASSFD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27277"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFH39_02635"
     CDS             <1..284
                     /locus_tag="VFH39_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924699.1"
                     /translation="ASKSKLVQGDILKLTISQDGSFLYKQIGPVPRKQVVGTLKLENG
                     HYLVEVGQKQYRVLLASVTYFKAKPGDQVSVNVPEDDSNADWAALEAAL"
     gene            358..945
                     /locus_tag="VFH39_02640"
     CDS             358..945
                     /locus_tag="VFH39_02640"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003300.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET6924700.1"
                     /translation="MAKLYFRYGTMNSGKSTALLQAAYNYEERGMRVLIIKSAVDTKG
                     GAAIVSRLGTTRNADILLRHDEPIRAQLDELAARHRLHCVLVDEAQFLAPEQVEELFW
                     FAVEADVPVMAYGLRTDFSTKAFSGSPRLLELAHELQELKTICRCGKKAVLNGRKIDG
                     KFVTEGGQVAIDDAPNIEYEALCAADYQRLVRRQA"
     gene            993..>2102
                     /gene="dnaX"
                     /locus_tag="VFH39_02645"
     CDS             993..>2102
                     /gene="dnaX"
                     /locus_tag="VFH39_02645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET6924701.1"
                     /translation="MGQALYRKYRSKSLDELQGQEHITKTLATAIKSGRIAHAYLFTG
                     PRGVGKTSTARILAYAINGLDYSEGQAHLDIVEIDAASNNSVEDIRDLREKVYVAPSV
                     GKYKVYIIDEVHMLSKAAFNAILKTLEEPPAHAVFILATTEAHKLPETIVSRTQRFVF
                     RPITTEKAVAHLKTIAKAEKIDITDDALQLLAAMGDGSFRDSISLLDQASGLGKTIDV
                     ADVQQLLGVPTAENITQLTDALVQLPLHLPTVLPALYALYDQGFQAASIASELSNQLR
                     KRIIDASFVPSQQHLELLAKLLDVPSRADPERYLEIILLGLGEATAQPVQTDVPKPKP
                     VAATPTKMPPFKPSSQAEKKHDRAEPAQEAAPKQPA"
BASE COUNT          516 a          614 c          567 g          405 t
ORIGIN      
        1 atgccagcaa gtctaagctc gtccagggcg atatcttaaa gctgactata tcgcaagacg
       61 gatcttttct gtacaagcag atcggcccgg taccgcgcaa gcaggtcgtg ggcacgctca
      121 agctcgagaa cggccattat ctggtcgaag tcggccagaa gcaatatcgc gtgctgctgg
      181 ccagcgtcac gtacttcaaa gccaagcctg gcgatcaggt cagcgtgaac gtccccgaag
      241 acgactcgaa cgccgactgg gctgcgctcg aagcagctct gtagcgtatc aattactgac
      301 taaatcgatg ccggcttcaa agccggtatt tttgaatgcg ctatacttat catcgatatg
      361 gcaaaactct atttccgtta cggcaccatg aacagcggca aatccacggc cctcctgcag
      421 gcagcttaca attacgaaga gcggggtatg cgagtattga ttataaaatc cgccgtcgac
      481 accaagggcg gcgcagccat tgtgtcccgg ctcggtacaa cccgaaatgc agacatactg
      541 ctgcgtcacg atgaaccgat ccgcgcgcag ctggatgagc tggcagcacg gcatcgcctg
      601 cactgcgtac tggtcgatga agctcaattt ttagctcctg aacaggtcga ggaactattc
      661 tggttcgcgg tggaggccga tgtgccggtg atggcttacg gcctacgcac cgacttttcg
      721 accaaagctt tcagcggcag tccacgtctg cttgaactgg cgcatgagtt gcaagagctc
      781 aaaaccatct gtcgctgcgg caaaaaggca gtgcttaacg gccgtaagat tgacggaaaa
      841 ttcgttaccg aaggcggtca ggtggccatc gatgacgccc cgaatattga gtacgaagca
      901 ctctgtgctg ccgattacca gcggttagtg cgacggcagg cataatttac agcagactcg
      961 cttcgatagc ccgcgtataa tgtaaggcag ctatggggca ggcattatat cgtaaatacc
     1021 gttcaaagtc gctcgacgag ctgcaaggcc aggaacatat aaccaagacg ctggcgaccg
     1081 ctattaaaag cggccggatt gcgcatgctt atctgtttac gggtccccgc ggtgtcggca
     1141 agacctcgac cgcccgtatc ctggcttacg ccatcaatgg tttggactac agcgaaggtc
     1201 aggcccacct ggatatcgtc gaaatcgacg ccgccagcaa caacagcgtc gaagacatcc
     1261 gcgatttgcg cgaaaaggtg tacgtcgccc cgagcgttgg taaatataag gtatacatca
     1321 tcgacgaagt gcatatgctc agcaaggcgg ccttcaacgc cattctgaag acgctggaag
     1381 agccgcccgc ccacgccgta ttcatcttgg cgaccaccga agcgcacaaa ctacccgaaa
     1441 ccatcgtcag ccgcacgcaa cggttcgtat tccgccctat caccaccgaa aaagccgtag
     1501 cgcacctcaa aactatcgcc aaggccgaaa agatcgacat tactgatgac gcactgcagc
     1561 tgttggctgc tatgggagat ggttcgttcc gcgacagcat cagcttactg gaccaggcca
     1621 gcggactcgg caagacgatt gacgtcgccg atgtccagca gctgctcggc gtaccgacgg
     1681 cggagaatat tacgcagctg acggacgcgc tggtgcaact gccgctccac ttgccgacgg
     1741 tacttccggc gctgtatgcg ctgtatgacc agggcttcca ggctgcatcc attgccagcg
     1801 aactctcgaa ccaactacgc aaacgaatca ttgacgcttc gttcgtaccg tcacagcaac
     1861 acctcgagct gctggcgaag ctgctagacg tgccaagccg ggcggacccg gagcgctacc
     1921 ttgagatcat cttgttggga cttggtgaag ccacagcgca gccggtgcaa acggacgtac
     1981 ctaagccaaa accggtcgcg gccacgccta ccaaaatgcc acccttcaag ccatcctcgc
     2041 aagcggagaa gaaacatgac cgtgcggagc cggcgcagga agccgctcct aaacaaccgg
     2101 cc
//
LOCUS       DASSFD010000116         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_170461, whole genome shotgun sequence.
ACCESSION   DASSFD010000116 DASSFD010000000
VERSION     DASSFD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170461"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFH39_02650"
     CDS             <1..360
                     /locus_tag="VFH39_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924702.1"
                     /translation="TDYIFRRLALDYLSFDDRLELGLANLEDLIADQTTLLVETPKAT
                     EPVAPAPVAAQPSVSQPAEEPVSPPVDEAPGMPSEEAKALVGDSTAPLCYNCGNQTQR
                     SGSCYVCTSCGSTTGCS"
     gene            515..1069
                     /locus_tag="VFH39_02655"
     CDS             515..1069
                     /locus_tag="VFH39_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924703.1"
                     /translation="MVSGRNTHIDQALEQRLQKHALSAVAKLTHVDLHEAVAVQRTSP
                     LLNEEIPLEEELINLEEAVKTYRLAMPNFQRYPKMRTHLHQEKRLILKHCLGLMMRLL
                     MKRPMHGAASSSDAGRAISRLVQIIVEDIRRNGIRLDTETKRIFEPFLDLKLRLMLSE
                     RPWSRFIGYQAKYSPVRVLIDHYS"
     gene            1116..1859
                     /locus_tag="VFH39_02660"
     CDS             1116..1859
                     /locus_tag="VFH39_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6924704.1"
                     /translation="MHLLIFGVINLAAAALSGAAGGGGGLVSLPLLVQLGLSPTAAIA
                     TSKFGGFGISAGSSLRFMRERLTERRPLIIFSIVGAVAAFGGSMLLVGFHTNQRLLQD
                     VMGVTILVVGIPLLYVRNMGLERKERSLPVRTAGFVLLFLGLVLQAALGGGVGSLQLI
                     VLMGCFGMTALVANATRRVMQLVVATISLAIFIAAGFVDFRYGAVGLVTSSIGGFLGA
                     HLAIKKGNKFVINLFAVTSAILALQLLWR"
     gene            1933..2199
                     /locus_tag="VFH39_02665"
     CDS             1933..2199
                     /locus_tag="VFH39_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924705.1"
                     /translation="MKLVVIYRPNSEHGRSVEDYLREFRSRYGDIKIEALDADTREGA
                     SMASLYDIMRCPAILALRDDGSTLNIWIDDSLPLMDELAGYAYS"
BASE COUNT          448 a          683 c          686 g          487 t
ORIGIN      
        1 acggactaca tcttccgtcg tctggccttg gactacttga gcttcgacga ccgtctggag
       61 ctcggtctgg ctaacctgga agacctgatc gctgaccaga ccaccttgct agttgagacg
      121 ccgaaggcta ctgagccggt cgctccggcc ccggtcgccg cgcagccgtc cgtcagccaa
      181 ccggctgagg agccggtctc cccgccggtc gacgaagccc cgggcatgcc gtccgaggaa
      241 gccaaggcgc tcgtcggcga cagcaccgca cccctgtgct acaactgcgg caaccagacc
      301 cagcgcagcg gcagctgcta cgtctgcacc tcgtgcggat caaccaccgg ctgtagctag
      361 aacctgagcc atcaggtcca caaaagaact cccccctagg gagttctttt tatttaccat
      421 ccgaggtata agcggagcgg taaaatgcga caaagcaagc ggagcgtaag cggtattgac
      481 catgcatggt atgttctgta aaatatctta cagaatggtg tcagggagaa acacgcatat
      541 cgatcaggcg ctggagcagc gccttcagaa gcacgcctta agcgcggtcg cgaagctgac
      601 gcatgtggac ttgcacgaag cggtcgccgt ccaacgcacc agcccgctgc tgaacgaaga
      661 aatccccctc gaagaagaac ttatcaacct ggaggaggcg gtcaagactt accggttggc
      721 catgccgaac ttccagcgct accccaagat gcgcacgcac ttacatcaag agaagcgact
      781 aatactcaag cactgcttgg gcctgatgat gcgcctgctg atgaagcggc cgatgcacgg
      841 cgcggcctca tcgagcgacg ccggccgggc catcagccgg ctggtgcaga tcatcgtcga
      901 agacatccgg cgcaacggca tccgcctgga caccgagacc aagcgtatct tcgagccgtt
      961 cctggacctc aagctgcggc tgatgctctc ggagcggcca tggagccggt tcatcggcta
     1021 ccaggccaag tactcgccgg tgcgggtgct catcgaccat tactcgtaga agcccacgcg
     1081 gactattaac ggcgctgcgc tataatcagt agtcaatgca tctgcttatc ttcggcgtca
     1141 tcaatctggc agcggcggct ttgagcggcg ccgccggcgg gggcggcggg ctggtgagcc
     1201 tgccattatt ggttcagttg gggctaagcc cgacggcggc cattgccact tcgaagttcg
     1261 gtggtttcgg catttcggca gggagcagcc tgcgcttcat gcgcgagagg ttgactgagc
     1321 gccggccgct gatcatcttc tctattgtcg gggcggttgc cgccttcggg ggcagcatgc
     1381 tcctggtcgg tttccatacc aatcagcgct tgctgcagga tgtcatgggc gtcaccatcc
     1441 tggtagtcgg tattccgctg ttgtatgtcc gcaacatggg cctggaacgc aaggagcgat
     1501 ctctaccggt gcgcacagcg ggtttcgttc tgctgttctt gggactggtg ctccaagctg
     1561 ctcttggcgg cggcgttggt tcgttgcagc tgatagtgct catgggctgt ttcggtatga
     1621 cagccctggt cgcgaacgcc acgcgccgtg tcatgcagct ggtggtggcg actatcagtc
     1681 tggcaatctt catcgctgcc ggtttcgtcg acttccgata tggtgcggtt ggcctcgtca
     1741 cctcgtctat cggcggcttc ctgggcgcgc atctggcgat caaaaagggc aataagttcg
     1801 tcatcaacct gtttgccgtt actagtgcta ttttggccct ccagctcctc tggcgctgaa
     1861 agtcgcgccc tatcttttgc agtttgtgat ccaagtcata cgtcgccgtt ccacttatgc
     1921 taagctgttg gtatgaagct cgtcgtcatc tataggccca actccgagca tggtcgctcc
     1981 gtggaggact acctgcgcga gttccggtcg cgctacggcg acatcaagat tgaggcgctc
     2041 gatgccgata cgcgcgaagg cgcctcgatg gccagcctgt acgacatcat gcgttgcccg
     2101 gccatcttgg ccctgcgcga tgacggctcg acattgaaca tctggatcga cgacagtttg
     2161 ccgctgatgg acgagctggc gggctacgcc tacagctagt gttgtaaaaa ccggcgtgag
     2221 cgcgtatatt taatctgtaa atgcgttctc cgcgaccgga gatgagcggc tatcaaccgc
     2281 gaagcagcgg gaagaaatcg aaag
//
LOCUS       DASSFD010000117         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1574594, whole genome shotgun sequence.
ACCESSION   DASSFD010000117 DASSFD010000000
VERSION     DASSFD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574594"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..803)
                     /locus_tag="VFH39_02670"
     CDS             complement(366..803)
                     /locus_tag="VFH39_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6924706.1"
                     /translation="MTSQAFSESVYLSFVTILMMGKLQLIKLGAEFDLTLVQTLTLLI
                     LAKTNEMPMSHLGKLYDCDASNVTGIVDGLEQKKLVSRRAHAKDRRVKMICLEPAGRK
                     LQAKLIKRLSETSGYLFDPLTETEQVRFAKLMEKISPDLTELT"
     gene            903..2369
                     /locus_tag="VFH39_02675"
     CDS             903..2369
                     /locus_tag="VFH39_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6924707.1"
                     /translation="MTDKARNHWLILILLALAQFMVVLDVSIVNVALPSIQRAFGLRE
                     SGLQWIVTAYTLTFGGFLLLGGRAADLFGRRKMFITGVILFGLVSLLDGLAQSGGMLI
                     ALRAAQGLAAALMSPAALSIVLVTYHEGHERNLALSVWGAVASGGAAAGVLLGGIITQ
                     YLGWRWNFFINVPVALLVALAAWRLVPLHESEETHNELDLPGAISITAGLMLLVYALV
                     EAPSHGWTSGSTIGYLAGAAALIAFFIRNERRAAHPLVPFDIFRLRNVVGANLTMMPI
                     VAGMFSTFFFTSLYVQTVLGFSPVKTGICFLATPICIALTAANVPRLVKRIGFKPILM
                     VAPLFTGASLFWLAHVRVLGNYWQDVLPGFILLGIGMGGTFIAITIAATSGVKGRESG
                     LASGLLNTSQQVGGALGLAILTGVATSATARYLHNLSGASTKLAPLAAEVHGFHAAFY
                     TASAFMLGAAIMATLILKQPKQFTAKDQSEAETAVSMH"
     gene            2426..>3594
                     /locus_tag="VFH39_02680"
     CDS             2426..>3594
                     /locus_tag="VFH39_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924708.1"
                     /translation="MSFLRRRGAEVVPPIEVESAPESTEPVDPHAFIGEHEDELYKSA
                     VNWGEIARNVRDFRSEYHDEIKAGLIDVWEANRQDPLPPDENLERETDRFGSTLSVYS
                     FVAARYTSTIVYDPARSHAKAFKDVIGMTRDLFMHIPDHTYNYVLRSKRFPTHRFMMR
                     QQALASAMFEADLHSNSILEGLVHAYEERPNDLRGLSELYADGAAENDRRYQELQSLI
                     AADWYRPDPSQSILRGFCQYLREQPEAYLPDFSEQVVQSERSTRLQAIRQMAAFALIA
                     SGAETVTDTGIIETLHRTYSHWDQVADLQQLFSDFVNGKVGQTEQAFQAIAGPRNRKD
                     MRMYPTAEEVEELRQNLIVPFLGQTDPVSRQHRRKASRVQQKAQKRNPFPEYSNL"
BASE COUNT          772 a         1042 c          976 g          804 t
ORIGIN      
        1 cgccggccta ttgcgaagcg tcgcccacta cagtaatcgc cactggctgt acgtgtccgt
       61 agcctgaaat ccgcaaaacg tcgttaatcg ttgacttttt gccaacgtta cgttgacaag
      121 gcttaagcgg gttcgctaaa attaggtcat tctaacagcg ggtgcgagag ccgtctgcga
      181 gataacaaaa aaccaaatta acaaaagagg tgttatgccg ggtttgaatt taggcaaacc
      241 ctggtttgac gcttccggag caaaaaatgt ccgtcaaaat aaaagtcctc aactccaaag
      301 agactcgcgt tacggttagc gtgcgccgcc cgcaatggca acgctacctg gagtactaag
      361 tcaggtcagg taagctcggt aagatccggg cttatctttt ccatgagttt ggcaaaacga
      421 acctgctcgg tttcggtcag cgggtcgaaa aggtagccgc tggtttccga cagacgcttg
      481 atcagcttgg cttgcagctt cctgccggct ggctcaaggc agatcatttt tacccggcgg
      541 tcttttgcgt gggcacggcg cgaaaccaat ttcttctgtt ccagaccgtc tactatcccg
      601 gtcacattac tggcgtcaca atcatacaac ttacccaagt ggctcatggg catttcgtta
      661 gtcttagcca atataagtag cgtcagcgtt tgcactaggg tcaggtcgaa ttcagcgccc
      721 agttttatga gctgcagctt gcccatcatc agaatggtca caaaggaaag atacaccgat
      781 tcagaaaatg cctgagaagt cataaaaatt atttgactat gtcaaccttt gagtattaca
      841 attaattata acattatcta ttgtaaacaa ataacgctga gggctcaagg agaaatctta
      901 ctatgaccga taaagcacgc aaccactggc tcatccttat tcttttagca cttgcccagt
      961 ttatggtggt gctggacgtc tcaatcgtga acgtcgccct gccctccata cagcgcgcct
     1021 ttggcctcag ggagtcgggc ctgcagtgga tcgttactgc ctatacgctg actttcggcg
     1081 gcttcttact gctcggtggt agggcagctg acctgttcgg gcggcgcaag atgttcataa
     1141 ccggtgttat cctcttcggg ttggtatcgc tgctggacgg cctggcccaa tccggcggca
     1201 tgctgatcgc tttgcgcgcg gcccaaggac tggcagcggc cttaatgagc ccggcggcgt
     1261 tatctatcgt gctggttact taccacgaag ggcacgaacg gaacctggcg ctgagcgtct
     1321 ggggtgccgt tgcctcgggt ggggcggccg cgggtgtgct gctcggcggc atcatcaccc
     1381 agtacctggg gtggcgctgg aatttcttta tcaatgtgcc ggtagcgctg ctggtcgcct
     1441 tggctgcttg gagattggta cccttacacg agagcgagga aacacataac gagctcgacc
     1501 tgccaggcgc catctctatc acggccggct tgatgctgct ggtatacgcc ctggtagaag
     1561 ccccgtcaca cggttggacc agcggcagta ccatcggcta tttggccggc gctgcagcgc
     1621 ttatcgcctt ctttatacgg aatgaacgcc gggcggccca cccgctggtg ccgttcgaca
     1681 ttttccgcct gcgcaacgtg gtcggcgcca acctgaccat gatgcccatc gtggccggca
     1741 tgttcagtac cttcttcttt acgtctctgt acgttcagac ggtgctcggt ttctcgccgg
     1801 tcaagaccgg catctgcttc ttggcgacgc ccatctgtat cgccctgacc gcagccaacg
     1861 tgccgcgact ggtcaaacgt atcgggttca agccgattct gatggtcgca ccgctcttta
     1921 ccggtgcatc gcttttctgg ttagcacacg tccgggtgct gggtaactac tggcaggatg
     1981 tgctgcccgg gttcatcttg ctcggcatcg gcatgggcgg cacgttcata gctataacca
     2041 tcgctgccac cagcggggtg aagggccgcg aaagcggctt ggcctccggc ctactcaaca
     2101 cctcgcagca ggtcggtggg gctcttggcc tggcaattct gaccggcgtc gctacctcgg
     2161 ctaccgcccg ctacttgcac aatctatctg gtgcgtcgac taagctggcg ccgctcgccg
     2221 ccgaagtgca cggctttcat gccgccttct ataccgccag tgccttcatg ctcggtgccg
     2281 ccatcatggc caccctcatc ctgaaacaac ccaagcagtt caccgcaaag gaccagtccg
     2341 aagccgaaac tgccgtcagt atgcactaag tcccccttcg cgctcggctt gccgccgcgc
     2401 gttcgcccat tgtatagtgg ttcaaatgtc cttcctaaga cgacggggcg ccgaggtggt
     2461 gccacctatt gaagttgagt cggcgccgga atccaccgag cccgtcgacc cgcatgcctt
     2521 cattggtgag cacgaagacg agctgtacaa gtctgccgtc aactggggag aaatcgccag
     2581 gaacgtgcgc gactttagaa gtgagtacca tgacgaaatc aaagccggcc tgattgatgt
     2641 gtgggaagcc aacaggcagg atccgctacc gccggacgag aacctggagc gcgaaacaga
     2701 ccgcttcggc tcgacacttt ccgtctattc gttcgttgcc gcgcgttaca cgtcgacaat
     2761 cgtttacgac cccgcccgtt cacacgcgaa agccttcaag gatgttatcg gtatgactcg
     2821 ggatctgttc atgcatatcc ccgaccatac ctataactat gtccttcgca gcaagcggtt
     2881 ccccacccat cgcttcatga tgcggcagca ggcactggcg tccgccatgt tcgaagcgga
     2941 tctgcacagc aacagtattc ttgagggcct tgtccacgct tatgaggaac ggccaaatga
     3001 cctaaggggc ctgtcggagc tctatgcgga cggtgccgcc gaaaacgacc ggcgctacca
     3061 agagcttcag tcactcatag ccgccgactg gtaccgcccc gatccgtcac aaagtatact
     3121 gaggggcttt tgtcagtatt tgcgcgagca accagaggct tacctgcccg acttctccga
     3181 acaagtagtg caaagtgagc gcagcacgag gctgcaggcc ataaggcaga tggccgcttt
     3241 cgcgctcatt gcgagcggtg ctgaaaccgt tacggacacg gggatcatag agacgctgca
     3301 ccgcacctat agccactggg accaagtagc cgatttacaa cagctgttca gcgacttcgt
     3361 gaacggcaag gtaggccaga ccgagcaggc ctttcaggca atcgctggtc cccggaatcg
     3421 taaagatatg cgtatgtacc cgaccgccga agaagtggag gaactgcgac agaatctgat
     3481 cgtacctttc ttgggccaaa ccgacccggt aagccgtcaa caccgtcgca aagcaagccg
     3541 cgttcagcag aaagcgcaga agcgcaatcc tttcccggag tactcgaacc tgga
//
LOCUS       DASSFD010000118         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_975253, whole genome shotgun sequence.
ACCESSION   DASSFD010000118 DASSFD010000000
VERSION     DASSFD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975253"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFH39_02685"
     CDS             <1..424
                     /locus_tag="VFH39_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924709.1"
                     /translation="FKANNFVLTGRGKVTVDAYLQTEDNIFVIGDNANTPYSGMAQTA
                     IHDADFVATNLRRRAAGQEFRSYKVKKPITVIPAGPRWAAVLWGSHRMYGWLGWVLRE
                     LGDLVGFHDYEPWPQASTQWLTELETEESCPVCLAASE"
     gene            complement(479..1312)
                     /locus_tag="VFH39_02690"
     CDS             complement(479..1312)
                     /locus_tag="VFH39_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6924710.1"
                     /translation="MADQPYVTLNNGVKMPQLGLGVWQASNDEAEAAVRWALEAGYRL
                     IDTAAAYGNEAGVGRAIGSSGIPREELFITTKLWNADHGYDEALRAFDKSMNELGLDY
                     LDLYLIHWPVPAKSQYKEAWQAFEKLHEDKRIRAIGVSNFLPEHLDDLITNTRLTPAV
                     NQIELHPYLQQHETRDFCAQHEIQIESWSPIGRGGDLLQDPVLKPIAETHGKSTAQVI
                     IRWHLQHGFVVIPKSVHQERIIENFDVFDFELSAEEMQVMNDMHSGTRLGPDPATANF
                     T"
     gene            1559..1945
                     /locus_tag="VFH39_02695"
     CDS             1559..1945
                     /locus_tag="VFH39_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6924711.1"
                     /translation="MASKTLTISSKGKRQVIDITEQIAQQVSEVDDGVVQVFVTHTTA
                     AVTTADLDPGTDADLLDFLQSMTPDVEWRHPHDPVHAPDHLWASLVGPSVGVPVQGGE
                     LALGTWQRIILVELDGPRDRQIIVTW"
     gene            complement(1935..2765)
                     /locus_tag="VFH39_02700"
     CDS             complement(1935..2765)
                     /locus_tag="VFH39_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924712.1"
                     /translation="MQAMAEAQTSSNNVEPATVGFPGDFVVMGVNDNRTEIHQIVGLA
                     GLNGTERQRRMQVASDQHGRYLRSENIGSGEADSVMHSQSIYKNTGRAAMWQHIVTVG
                     DNLPKVAKIMRGSGHPDLQFMGALLDPPDGTPQRRRVFGLIKQGRERHGRQNIILLHA
                     FASRPAPLGDGIGHAHLRAVELPYKIEGYEGEGQAMTQFDAAAPEPLVPYTRRPTTVR
                     MGHELTESMQAVYERYGTEYVVALGGVSIKLGLDADKPINLQRFTDYAVLNPAVSITK
                     "
     gene            complement(2817..3014)
                     /locus_tag="VFH39_02705"
     CDS             complement(2817..3014)
                     /locus_tag="VFH39_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924713.1"
                     /translation="MERRGISIGTVIYLIIGLIVAANQGYLGQLTTVSGILSAILAIL
                     LWPLLLFGVNLHVALSMRATR"
     gene            3269..3445
                     /locus_tag="VFH39_02710"
     CDS             3269..3445
                     /locus_tag="VFH39_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924714.1"
                     /translation="MGTVAFPVAMLPHIEARNGAQLAEPGDEFDQVASLLADTFARAG
                     FIEGATIKESTLHP"
     gene            3491..>4457
                     /locus_tag="VFH39_02715"
     CDS             3491..>4457
                     /locus_tag="VFH39_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924715.1"
                     /translation="MADQLAIAEYTKAVQEQFNSGQAREHGYRPALEHLMRSFDNVGA
                     TNDPRRLEFGNPDMIFYRANNHDLILGYAEAKDITADLDKTAKTEQLHRYAGYNKLFL
                     TNYLDFIFYRNGEEYERISIGRLRGQTIDFNLAEYARLAHELHAFLESAPEPIKNGKS
                     LARIMGQKARRLRDNVSRYMTEQSDGNQELEKIFDMMKELLVHDLSQEKFADMYAQTL
                     VYGLFTARYNDSTPEDFSRREASELVPASNPFLREFFAHIGGIHFDKRLAHIVDELCA
                     VFAVSNVQHLVEKHLRLFEVENEKDPIIHFYEDFLKEYDPSERKRM"
BASE COUNT          968 a         1284 c         1193 g         1012 t
ORIGIN      
        1 cttcaaggcc aataatttcg tactcacggg gcgcggcaaa gtaacggtcg acgcttactt
       61 acagaccgag gacaacatct tcgtcatcgg cgacaacgcc aacacgccgt acagcggcat
      121 ggcgcaaaca gccatccacg acgccgattt cgtcgccacc aacttgcgac gccgggccgc
      181 tggccaggag ttcaggagtt ataaggtcaa aaagcccatc accgtcatac ccgccggtcc
      241 gcgttgggcc gcggtgctgt ggggcagtca ccgcatgtac ggctggctcg gttgggtact
      301 gcgcgaactc ggggatctgg tcggtttcca cgactatgag ccgtggccgc aggccagcac
      361 ccagtggcta accgagctcg agaccgaaga gagctgcccg gtctgtttgg ccgccagcga
      421 gtagctggtt ctaccgcacc agatgccagc ggtgcgctaa acccaccagt cgcgtacttc
      481 aggtaaagtt ggctgtcgcc gggtcggggc ctaagcgcgt gccgctgtgc atgtcgttca
      541 tcacctgcat ttcctcggca ctcagctcga agtcaaagac atcgaagttc tcgattatgc
      601 gctcctggtg caccgactta ggaatcacta cgaagccgtg ctgcagatgc cagcggataa
      661 ttacctgggc cgtactcttg ccgtgggtct cggcgattgg cttaagcacc ggatcttgta
      721 acaggtcgcc gccgcgaccg atggggctcc acgactctat ctgtatttcg tgctgggcac
      781 agaaatcgcg cgtctcgtgc tgttgcaggt acgggtgcag ctcaatctgg ttcaccgccg
      841 gcgtcaggcg ggtattggtt ataagatcat ccagatgttc cggcaagaag ttactgacac
      901 cgatggcccg gatgcgcttg tcctcgtgca gcttttcgaa cgcctgccag gcctctttgt
      961 actggctttt ggccgggacc ggccagtgga tgagatacag gtccaggtag tccaacccta
     1021 actcgttcat gctcttgtcg aacgcccgta gggcttcgtc gtagccgtgg tcggcgttcc
     1081 acaatttagt cgtgatgaac agttcttcgc gcggaatccc gctgctgcca atggcccggc
     1141 ccacaccggc ctcgttcccg taggccgccg ctgtatcgat caaccggtag ccggcttcga
     1201 gcgcccaacg gacggcagcc tcggcctcgt cgttactggc ttgccagaca cctaagccca
     1261 gctgcggcat cttcacgcca ttgttcaacg tcacgtacgg ttggtccgcc atgtttccct
     1321 ccatcggttg gttagcagtt attagtcgcg ggtgcggtcg cgcagatcct gccattgggc
     1381 ctgcgcttcg gcatccatcg tacggaactg atcatcggca atcttgcgcc agtttttctt
     1441 cgacatactt tatgtctccg tttattttgc tgacttgtcc attctatgcc gtataacgcg
     1501 gccgcgaagt aatatctttt actgtcagct ttttgggctc acgctaggct ggaacgcaat
     1561 ggccagtaag acacttacga tttcatcaaa aggcaagcgg caggttatcg acatcaccga
     1621 gcagatagcc cagcaggtga gtgaggtcga tgatggcgtc gtgcaggtgt tcgtgaccca
     1681 cacgaccgcc gccgtcacca cggcggatct tgatcccgga acggacgccg atctgctcga
     1741 cttcttgcag tctatgacgc cggacgtaga gtggcgccac ccgcacgacc cggtccatgc
     1801 gccggatcat ctgtgggcca gcctcgtcgg gccgtcggtg ggggtgccgg tgcagggcgg
     1861 tgagctggcg cttggcacct ggcaacgcat cattctggta gaactggacg gtccgcgcga
     1921 ccgccagatc atcgtcactt ggtgatgcta acagccgggt tcaacaccgc gtaatcggta
     1981 aagcgttgca ggttgatggg tttgtcggcg tccagaccca gctttatcga cacgccgccc
     2041 agggccacaa cgtattcggt tccataacgt tcatagaccg cttgcataga ctccgtcagt
     2101 tcatggccca tccgcacggt cgtcggccgt cgggtgtacg gcaccagtgg ctcaggcgcc
     2161 gccgcgtcga actgcgtcat agcctggccc tcgccttcgt aaccctctat cttgtacggt
     2221 aactcgactg cccgcaagtg ggcgtgacct ataccatcgc ccagcggtgc cggcctggag
     2281 gcgaaggcgt gtaacaatat gatgttctgc cggccatgcc gctcacgacc ctgttttata
     2341 agaccgaata cccggcggcg ctgtggcgtg ccgtccggcg gatccagcag tgcgcccatg
     2401 aattgtaggt cagggtgacc gctgccgcgc ataatcttgg ccaccttcgg cagattgtcg
     2461 cccacggtca ctatgtgctg ccacatggca gccctgccgg tgttcttgta gatactctgc
     2521 gagtgcatca cgctgtcggc ttcgcctgag cctatgtttt ccgatcgcag gtagcggcca
     2581 tgctggtcgc tggccacctg catccggcgc tgacgttcgg taccgttcag cccggccagg
     2641 ccgacaatct ggtgtatttc ggttctgtta tcgttcactc ccatcactac aaagtcgcca
     2701 ggaaacccga cagtcgctgg ctccacatta ttagaagagg tttgcgcctc cgccatggct
     2761 tgcacctccg attactacct atatcttccc atagaaaggc gcttatggca actgcattat
     2821 ctggtagcgc gcatgctgag cgctacatgc aggttcacac cgaacagcag cagcggccac
     2881 agcaatatcg ccaatatggc cgacagtata ccgctcaccg tcgtcagctg ccccaagtag
     2941 ccttgattag cagcaactat cagtccgatt atcagatata tcaccgttcc gatcgatatc
     3001 ccacgtcgtt ccatgctatc tccttttgca ttaatgctct gcctaactat gcccaacccc
     3061 aagcgtaacg gcagtagcaa tttttacagc cgaaacggca agcgtaactt cttgtttgca
     3121 gctgcgcaaa tacatgctaa gctacggcga tatgatgaga aagcgatcta ctatgccgcg
     3181 agcagagctg ttgtccgtgc cgttcaggca gcgagtacgc gaacgcgccc attatctcag
     3241 ccgagtcctc ctagacgtcg gctgcgccat gggtaccgtc gcctttcccg tggctatgct
     3301 gcctcacatc gaagcaagaa atggggccca attagctgaa ccgggtgacg aattcgatca
     3361 ggtagcaagt ttgctggccg atactttcgc cagagctgga ttcattgaag gcgccaccat
     3421 taaagaatcc acgctccacc cttaaccgca ccgttttaaa acagatacaa aagtgctatg
     3481 ctgaagccaa atggcagacc agctagcaat tgctgaatat actaaggcag ttcaggagca
     3541 atttaactct gggcaagccc gtgagcacgg ctacaggcca gcgcttgagc atctgatgcg
     3601 ctcgtttgat aatgtcggcg cgactaatga ccctcggcgg ttggagttcg gaaatcccga
     3661 catgattttc taccgcgcca ataatcatga cctaattttg ggttatgcag aagcgaaaga
     3721 tattacggcc gacctagaca aaactgctaa gactgagcag ctacatcgtt acgcgggcta
     3781 caataaatta tttcttacca actatttaga cttcatcttt taccgcaatg gcgaagagta
     3841 tgaacgcatc agtattggca ggcttcgtgg ccaaactatt gactttaacc tagcagaata
     3901 cgcacgtctg gcgcacgagt tgcatgcctt cctagaatca gcacccgaac caattaaaaa
     3961 cggtaaaagc ctggcgcgaa taatgggtca gaaggcacgg cgtttacgag ataatgtcag
     4021 ccgctatatg actgagcaaa gtgacggcaa ccaagagctc gaaaagatat tcgatatgat
     4081 gaaagagctt ctggtccatg atctaagcca agaaaaattc gcagatatgt atgcccagac
     4141 gctggtgtat gggctgttca cggcccgcta taacgactca actccggagg attttagtcg
     4201 gcgcgaggct agcgaattgg tgccagccag caatccattt ttacgggagt tctttgcaca
     4261 cataggcggg atacatttcg ataaacgcct ggctcatatc gtagatgagc tctgtgcagt
     4321 ctttgccgtt agcaatgtgc aacacctagt cgagaaacac ttgcggctat tcgaagtgga
     4381 gaatgaaaaa gacccaatca ttcacttcta tgaagatttt ttgaaggaat acgacccttc
     4441 cgagcgcaaa cgcatgg
//
LOCUS       DASSFD010000119         7067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_514688, whole genome shotgun sequence.
ACCESSION   DASSFD010000119 DASSFD010000000
VERSION     DASSFD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514688"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFH39_02720"
     CDS             complement(<1..193)
                     /locus_tag="VFH39_02720"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HET6924716.1"
                     /translation="MKYLIIRHAQTDSTRLNRHLDGPEGAPLNEAGRMAARALRDELT
                     AAGLDVPHVKVAISELVRTR"
     gene            complement(438..731)
                     /locus_tag="VFH39_02725"
     CDS             complement(438..731)
                     /locus_tag="VFH39_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924717.1"
                     /translation="MAERSHGSSSMPENYDPFTDLSLVWKEPKGTAERSIDLLALRMF
                     EAADVEEGVCDTDRGLELKLPDEPTPRFAPEDLTTLGVEWVTIVDNLPKPDQK"
     gene            complement(1103..1179)
                     /locus_tag="VFH39_02730"
     tRNA            complement(1103..1179)
                     /locus_tag="VFH39_02730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1143..1145),aa:Val,seq:gac)
     gene            1836..2240
                     /locus_tag="VFH39_02735"
     CDS             1836..2240
                     /locus_tag="VFH39_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924718.1"
                     /translation="MVCIYCGSGTQVINSRLQKRVNQVWRRRKCVACGAVFSTHEAAA
                     YDGAWRVKDVRGKLVPFSRNKLLVSLYKSLEHRQQPLADAEGLLDTIMGKLIKTAQNG
                     LLDRKDIIKAASGVLKRFDTPASVHYAAFHRG"
     gene            complement(2237..2773)
                     /locus_tag="VFH39_02740"
     CDS             complement(2237..2773)
                     /locus_tag="VFH39_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924719.1"
                     /translation="MSNSEALHFPERDPAYLALIDQYEATARETMNRYAEYADTYPHC
                     SSLRLIPRDNQWSPFGEEELSIERRTSINISKATEIYPSLIVHDVVWDDYEHSAGTST
                     TYTVFDGYLLRTIEEFDKAYSSEFDEPYDTPHTEVLEADEVKKLMRRLEQARPRFMEA
                     PSLSDRLGQLVSRLLGSN"
     gene            complement(2815..4449)
                     /locus_tag="VFH39_02745"
     CDS             complement(2815..4449)
                     /locus_tag="VFH39_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6924720.1"
                     /translation="MTDIEIPLERERHGWYRFFEILPGLLTWTLLFTPLILSFINVTA
                     AAFFILAYVLIYFTRSIAVDVRAIQGHRMLNQYKKINWNELIADVQAGQAPPADHPRP
                     RWHLNTLMRLQAQPSPVDPNQLYHAIIIATVNETKDVLEPTLHAILHSNYDLKRVILV
                     FAYEARAGQEAETRVHELLNEYRGHFHHAMAVKHPFGIPDEVIGKGGNVTYAARELQR
                     YLEDQHIDPVNVVVTTLDSDNRVDKDYLSALSYLYCVAPDPIRASYQPLPMYTNNIWD
                     APAPMRVIATGNNFFYIVLTQRPHLERNFSAHAQSMRALIDMDFWSVRTVVEDGHHFW
                     RSYFFYDGDYRVYPLTTPIYQDAVLAATYWKTIRAQFIQLRRWTYGASDVAYVASKGF
                     RRGSKVPKFDVIAKWLRLLEGHVSWGGGAIIMLTAGFIPAFINPQSYAANELPLILSR
                     INTVGLLGLLASLYVSMKTLPPRPPRYKRHRSLFMLLQWVYLPVTTICFGSLAALNSQ
                     TRLIFKRYLSKFDVTEKAVVKTTGERVSSEADPSRR"
     gene            complement(4498..4863)
                     /locus_tag="VFH39_02750"
     CDS             complement(4498..4863)
                     /locus_tag="VFH39_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924721.1"
                     /translation="MTSTAKYFHDRLILLLLSTNVFLALANSIFVLVQLSSNHGNGYI
                     VQCRDCSNIGNINRFTNGGVGELLAFIVFALAVAVIHIVLSLRTYRIHRQLAVIILAL
                     GTLLLIMTAIVGNALLVLR"
     gene            complement(4916..4990)
                     /locus_tag="VFH39_02755"
     tRNA            complement(4916..4990)
                     /locus_tag="VFH39_02755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4955..4957),aa:Gly,seq:ccc)
     gene            complement(5143..5218)
                     /locus_tag="VFH39_02760"
     tRNA            complement(5143..5218)
                     /locus_tag="VFH39_02760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5183..5185),aa:Thr,seq:cgt)
     gene            complement(5264..5779)
                     /locus_tag="VFH39_02765"
     CDS             complement(5264..5779)
                     /locus_tag="VFH39_02765"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HET6924722.1"
                     /translation="MAVFHAKTKLDRAFEISLLLKGLDGLLETLSGIAFLFIKPAWVV
                     DLAHAIVGYHPTGFIGEHILTSAQHFGHGTAIFASLYLLSHGLVKLVLVVEILREHLW
                     AYIGLIVVTGGFIIYQLYHIFFKHPSISFIALTIFDVIVVYLTVREYGHQKERLGKKH
                     AAEKGESASAD"
     gene            complement(5826..6164)
                     /locus_tag="VFH39_02770"
     CDS             complement(5826..6164)
                     /locus_tag="VFH39_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924723.1"
                     /translation="MATENIPETIPDVPDVAEQSAMMLATGPYGMAFAGLALWLYGYG
                     AGVKDNIDMAIARLDRFYGRQSPALRASEAPAFEAIERPDQFAKPEYFAFEVAARAAK
                     QRHAGSGEPA"
     gene            complement(6233..>7067)
                     /locus_tag="VFH39_02775"
     CDS             complement(6233..>7067)
                     /locus_tag="VFH39_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6924724.1"
                     /translation="LTLWLMRGLAPEPAVQGSPKIDSPGAVLCTLGLGGTVFALIEQP
                     SYGWGSPAIWLPLICGLLLLVSFVWYERRTPQPMLPLSLFKRRNFSVGNIATAAIYGG
                     LSIATFLIVIYLQQVGGFSALHAGIALLPVTVVMFLLSPRFGALAGRTGPRLFMALGP
                     MVAAGGFLLMLRVRPELVYLTQLLPGVLLFALGLSMTVAPLTAAILGDVDTGRAGVAS
                     AVNNAVSRIAGLITVAALGLITGPHLSPVGFRRALIATAILMTTGGIISLIGIRNPHR
                     A"
BASE COUNT         1601 a         1899 c         2021 g         1546 t
ORIGIN      
        1 gtcgagtccg cacgagctca gaaatcgcta cttttacatg cggtacatct aggcccgcgg
       61 cagtcagttc atcacgcagc gcacgcgcag ccattctgcc cgcctcgtta agcggtgccc
      121 cttccggccc gtccaaatgc cggttcagcc gcgtactatc tgtttgagca tgtcggatta
      181 tcagatattt catgacctaa tcttaccaat aaaaaaacgg ttgggtgatc tcgcatgtca
      241 tccaaccgtt ttacttttcg atgtgccctt gctaacacct agtgtagcac ggactgaaac
      301 acatcgcaaa acctcaccta ccaataagct taacaggacc agccatttaa tgctagtact
      361 gaaatgatgc atttcttact attccacctg tggatagccg attaaattct atacctttta
      421 tggtatttat ttgggaatta tttctggtcg ggctttggta agttatcgac tatggttacc
      481 cactccacgc ctaaagtcgt aaggtcctcg ggagcaaagc gaggcgtagg ttcgtcaggt
      541 agcttcagct ccaagcctcg atcagtgtcg cagacacctt cttctacatc ggccgcttcg
      601 aacatgcgaa gcgccaacag gtcgatactt cgctccgcgg tgcctttagg ctctttccat
      661 acgaggctaa gatcggtaaa tggatcgtaa ttctccggca tggatgatga gccatgcgaa
      721 cgctctgcca taactaactc cttagttatg catattgtac catgtttgcc tcgtttattc
      781 cactcgatag cggaggcgat atcgctgcct gcgcagccgt atcgcgccat ttcaaaaaac
      841 aaaatccgac cccaagggtc ggcttctgtt tttggtgggc gatataggat agcggctgcg
      901 ccttctccgc tgcgccgtgc tcttattgtg ggatggggcg atagacactg gcgtttcgtc
      961 tgcacgccat gctcgaagca agcttctgcg cgtggcgctt gccgtaggat ccgcccctaa
     1021 ctcgcttcgc tcgcacatag gttcgaatcc catcctgcgc cacaaaaaca aaaatacccc
     1081 tatacacagg ggtatttttg tttggtgggc gatataggat tcgaacctat cacctccaca
     1141 acgtcaatgt ggcgctctag ccaaatgagc taatcgccca agtccatgag gcaaacctac
     1201 ggtttttctc atcgcgccgg gccgcggaat gaatccgacg actcgggctg ctcttttccc
     1261 taaatcgcaa acgctgatta tgggatcaga gttaggctcc attgaatata gcgtattacc
     1321 cctggtaacg caagccccta ataactgcct tacctctgtt acaaaacgct tgtgtttacg
     1381 ggcgtaatat tgacaagtat gcctacaact ggtagcatag acaggttaaa cgggactttg
     1441 tgtgagtacc aacaaatcaa gcgtgcttga tgagttccag ttgttttacg aggagctagg
     1501 cgtctcgaaa ccgactggtc gaatccgcag attacgtgcc gcctgagcct ccgggcgacg
     1561 gtcgcacaat atacccctcc agctacccag ccggcggggt atttttaatt actccggcct
     1621 atggaagata gcaacctacc actgttaagg agcggccggg aatggtgcag tgtgagatgt
     1681 aatgtgagca gcgaagcgca gcgtaatttc ggtacgtgag cattgcagcg cagcatttcg
     1741 ctcgcaatgt gccgtttcgg gcagctccgc gcttatcccc aatggggaat agttttgcac
     1801 tagcattttg aatagcgtta tgacaatatg tagtcatggt ttgtatatac tgtggctctg
     1861 gcactcaagt catcaattcc cgcctccaga agcgggtaaa ccaggtttgg cgacgtcgca
     1921 aatgcgtcgc gtgcggggcg gtcttcagca cgcacgaagc agcagcctac gatggcgcct
     1981 ggcgcgtaaa agatgttcgg ggcaagttgg tgccattcag ccgaaacaaa ttactggtaa
     2041 gcctgtataa aagcctggag caccggcagc aaccgctggc cgacgccgag ggcctgctgg
     2101 acacaatcat gggtaagctg ataaaaacgg cgcagaacgg cctgctggat cggaaagata
     2161 tcataaaagc cgcatcgggc gtactgaaac gtttcgatac gccggccagc gtgcactacg
     2221 cggccttcca ccggggctag ttgctgccga ggagacggga tactaactgg cccaaacggt
     2281 cactgagcga aggggcttcc ataaagcgcg gtcgggcctg ctccagacgg cgcatgagtt
     2341 ttttgacctc gtcagcctcc agaacttcag tatgaggcgt gtcgtatggc tcatcgaatt
     2401 cagacgaata agccttatcg aattcctcga tagtccgcag caggtagccg tcgaataccg
     2461 tataggttgt ggaggtaccg gcgctatgtt cgtaatcatc ccacacgaca tcatggacaa
     2521 taaggctcgg atatatctca gtggccttac tgatatttat actcgttcgt cgttcaatag
     2581 acagttcttc ctcaccgaag ggcgaccact ggttgtccct ggggattagt ctgagggatg
     2641 agcaatgcgg atacgtatcg gcgtactcgg cataacggtt catcgtctcc ctggctgttg
     2701 cttcatactg gtctatgagt gcaaggtatg ccggatctcg ctccgggaaa tgcagcgctt
     2761 cgctatttga catagctgtt attttaccac tttactggta tttttcaata attgctatcg
     2821 gcggctgggg tcggcttcgg aggagacgcg ctcaccggtg gttttgacga ctgctttctc
     2881 ggtgacgtcg aacttgctga ggtagcgttt gaagataagc cgggtctgcg agttcagcgc
     2941 ggccaacgaa ccgaaacaga tggtggtcac cggcaggtaa acccactgca gcagcatgaa
     3001 cagcgaacgg tggcgcttgt agcgcggcgg gcgcggcggc agcgtcttca tggacacgta
     3061 cagcgaggcc agcaatccga gcaggccaac ggtgttgatg cggctaagga tgagcggcag
     3121 ctcgttggca gcgtagctct gcgggttgat gaacgccgga atgaagccgg cggtgagcat
     3181 gatgatggcg cctccacccc aagaaacgtg gccctcgagc agacgcagcc acttggctat
     3241 gacgtcgaac ttcggcacct tgcttccccg tcggaagcct ttgctggcga catacgcgac
     3301 gtcggaggcg ccgtatgtcc agcggcgcag ctggatgaac tgggcgcgga tggtcttcca
     3361 gtaggtggcg gccagcacgg cgtcctggta gatgggcgta gtgagcgggt acacccggta
     3421 gtcaccatcg taaaagaagt agctgcgcca gaagtggtgg ccgtcctcta ccaccgtccg
     3481 gacactccag aaatccatgt ctataagggc gcgcatgctc tgggcgtggg cgctgaagtt
     3541 acgctccagg tgggggcgct gggtgagcac gatgtaaaag aagttgttac cggtggcgat
     3601 gacgcgcatc ggagccggcg catcccagat gttgttggta tacatcggca gtggctggta
     3661 gctggcccgg ataggatccg gcgcgacgca gtacagatag ctgagggcgc tcaggtagtc
     3721 cttatccacc cggttgtcgc tgtccagcgt ggtaacgacg acattgacgg ggtcgatgtg
     3781 ctggtcctcc agatagcgct gcagttcgcg ggcggcgtac gtgacgttgc cgcctttgcc
     3841 gatcacctca tccgggatgc cgaaggggtg cttcactgcc atggcgtggt ggaaatgacc
     3901 cctgtactca ttgagcagct catggacgcg ggtctcggcc tcttgccccg cccgggcctc
     3961 gtaagcgaac accaggatga cacgcttgag gtcgtagttc gagtgcaaga tggcgtgcag
     4021 cgtcggctcg agcacatctt tggtttcgtt caccgtggcg atgatgatgg cgtggtacaa
     4081 ctggttggga tcgaccggcg acggctgggc ctgcaggcgc atgagcgtgt tcaggtgcca
     4141 acgcggccgc ggatgatccg ccggaggcgc ctgtcccgcc tgcacgtcgg caatcagttc
     4201 attccaattg atcttcttgt actggttgag catgcggtgc ccctggattg ccctgacatc
     4261 gaccgcgatg gaacgcgtaa agtaaatcag cacgtacgcc aagatgaaga acgccgccgc
     4321 cgtgacgttg atgaagctta aaatgagcgg cgtgaacaac agcgtccagg tcagcagccc
     4381 cggcagaatc tcgaagaagc ggtaccagcc atggcgctcg cgttcgagcg gtatctctat
     4441 atcggtcatt tactctagtt tataggtttt agctaagact taacagaggt ttttgggcta
     4501 gcgcagcacc agcagcgcat tgccgacgat ggccgtcatg atgagcagca gcgtgcccag
     4561 tgccaggatg atgaccgcca gttggcggtg gatgcggtag gtgcgcaggc tgagcacgat
     4621 gtggatgacc gccaccgcca gcgcgaagac gatgaacgcc agcaactcgc ccaccccacc
     4681 gttagtaaag cggttgatat taccgatatt gctacagtcc cggcactgca ctatgtaacc
     4741 gttgccgtgg ttggaactca gctgaaccag cacgaagatg ctgttggcca acgccaaaaa
     4801 cacgttggta ctcagcagca gcaagatgag ccggtcatga aaatacttag cggtcgaagt
     4861 cataaaatct agctactatt gtagcattct tggggttcag tttacggata aaacgtggtg
     4921 cgggtacagg gagtcgaacc ctggtctcaa ccttgggaag gttacataat agccgttata
     4981 cgacacccgc gtataccact tcgccgcgtc tggtaatcaa caatgcggtg cgtaatccgg
     5041 atttagtccg gattcgcagc tgccgctagt ggaaaaagag cagccggagt tctttggaac
     5101 gccgcgcgtc gagaaaaaac cgaaggtttt gtcttgagtt tgtggagcca cctgggagga
     5161 tcgaactccc gaccttcgcg ttacgaatgc gatgctctac cagctgagct aaggtggccg
     5221 attgccgttc ctaggaacgc gtccgtaaaa ttatagcaga gatttaatcg gcggaggccg
     5281 attcaccctt ctcggccgca tgctttttgc cgaggcgttc cttctggtgg ccgtactcac
     5341 ggacggtgag gtaaaccact ataacgtcga agatggtcag ggcgatgaag ctgatgctcg
     5401 ggtgcttgaa gaatatgtgg tacagctggt agatgatgaa gccgccggtg accacgatga
     5461 gcccgatgta cgcccacaag tgctcgcgca atatctcaac caccagcacc agcttgacca
     5521 aaccgtggga taacagatac agggaggcga aaatggccgt gccatgccca aaatgctgcg
     5581 cggatgtaag gatgtgttcg ccgatgaagc cggtcggatg atacccgact atggcatggg
     5641 ccaagtcgac cacccaagcg ggtttgatga acaaaaaagc gatgccgctg agcgtctcca
     5701 aaagaccgtc gaggcctttg agtaacaggc ttatttcgaa cgcgcggtcg agtttggtct
     5761 tggcatggaa tacggccatc gtgatttaag tgtagcaggc ttgattaaga gaaccttaaa
     5821 gtggtctatg ctggctcgcc agaaccggcg tggcgctgct tggcagcacg ggcagcgacc
     5881 tcgaatgcga aatattcggg cttggcaaac tggtctggcc gctcgatggc ctcaaaagcc
     5941 ggtgcctcgc tagcccgtag tgctggggac tgtctgccgt agaaacgatc gagccgcgct
     6001 atcgccatgt ctatattatc tttcacgccg gcgccgtagc cgtagagcca tagagccagg
     6061 ccagcaaaag ccataccata gggaccagtc gccagcatca tggcgctttg ctcggccacg
     6121 tcgggaacat caggtatcgt ttcggggata ttttcggttg ccatgactat attgaatcat
     6181 cttgtgatat cttcagttgg caattataca ataatatgtt ggatattcaa tattacgcac
     6241 ggtgcgggtt gcggataccg atgagcgaaa tgataccgcc cgtagtcatc agtattgccg
     6301 tggcaatgag tgcgcggcgg aagccgactg ggctcaggtg cggaccggtt atgagcccta
     6361 gcgcggccac cgtgatgagc ccggcgatgc gcgacacagc gttattcacc gccgatgcca
     6421 cccctgcccg tccggtgtcg acatcgccta agatggccgc cgtcaacggt gcgaccgtca
     6481 tgctcaggcc gagcgcgaac aacaacacgc cgggcaggag ttgcgtcaga tatacaagct
     6541 caggtcgcac ccgcagcatc agcaagaagc cgccggccgc caccatgggg ccgagtgcca
     6601 tgaacagccg cggtccggta cgaccggcca gcgcgccgaa gcgcggcgac agcaggaaca
     6661 tcacgaccgt tacgggcagc aatgcgatgc cggcatgcag cgcgctgaag cccccgactt
     6721 gctgcaggta gatgacgatc aggaacgtcg caatcgacag accgccataa attgctgcag
     6781 tcgctatgtt gccgaccgag aaatttcggc gcttgaacaa agaaagtggc agcataggtt
     6841 gcggagtccg tctctcgtac cacacaaagc ttaccagtag taacaacccg catatcagtg
     6901 gcagccagat ggccggcgag ccccagccgt aagacggttg ttcgataagc gcgaaaaccg
     6961 tgccgcccaa acccagcgta cacaaaaccg caccaggcga atcaatcttc ggactgccct
     7021 gcacggctgg ttcgggcgcc aggccgcgca tcagccacag cgtgagt
//
LOCUS       DASSFD010000120         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1482759, whole genome shotgun sequence.
ACCESSION   DASSFD010000120 DASSFD010000000
VERSION     DASSFD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482759"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFH39_02780"
     CDS             complement(<1..360)
                     /locus_tag="VFH39_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924725.1"
                     /translation="MAQDTISLTLEPRKVTGKQVRQLRREGTVPAVIHDHGKDSVVVQ
                     GDYMAVLKVWQQAGKHHPVNLQAGDKTFTALIKTADFDPKKHQLRHVVFNAVRANEKV
                     EAEVPIHPRYSEGNEISP"
     gene            521..1132
                     /locus_tag="VFH39_02785"
     CDS             521..1132
                     /locus_tag="VFH39_02785"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6924726.1"
                     /translation="MFDVSGIIAAGGLALIALIIFAEVGLMVGFFLPGDTLLLSAGIF
                     AAQGKLPIEWTIPLVALAAILGDNTSYFIGHKLGPRLFRKKDGLIFRQEYVEKAEKFY
                     EIYGNKTALLAHWLPVIRTFAPLLAGVGKMRYRTFAIYDAIGDIGWAAAVTLLGYFIG
                     TRIPNIDHYIMLAVAAAVVISFGPTVYHLIKAVRERRRTPESE"
     gene            complement(1302..>4036)
                     /gene="uvrA"
                     /locus_tag="VFH39_02790"
     CDS             complement(1302..>4036)
                     /gene="uvrA"
                     /locus_tag="VFH39_02790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6924727.1"
                     /translation="LVVITGLSGSGKSSLAFDTIYAEGQRRYVESLSSYARQFLGLME
                     KPDVDQIDGLSPAISIDQKSTSRNPRSTVATVTEIYDYLRLLFARIGTPHCPVCGNPV
                     ERQTTNAIVDQVAALPEGARLMILAPVVVDKKGAFEHIPEQYQRAGFARVRVDAVVYA
                     LDEFPQLDKNYKHTIEIVADRLVNNEESRSRLVQSVEQALDVAEGKLNVLNADTDELT
                     SYSLMYACPDHPDVVIPELEPRTFSFNSPHGACPVCTGLGSRLEVDPELVIPNGRLTI
                     AEGAIRPYNRINVDNWYMKKLEAVAGKYGFSLHVPTSEISAENMQRIMYGTGGEKFRV
                     QIGMGRSFETTYEGVVPNLERRHRETDSDFIRRDIERFMQEKPCHMCKGLRLKPEALA
                     ITVGGQSIMDVCTLSIDDAIDWFNHLKLNATEQKIANLILKEICARLQFLQDVGLNYL
                     TLLRSATTLSGGEAQRIRLATQIGSGLQGVLYVLDEPSIGLHQRDNERLIRTLKHLRD
                     LGNTVIVVEHDEDTIRTADYLLDIGPGAGIHGGKVVANGTPEEVAAVKESITAQYLAG
                     HRAIAVPKKRRPGNGKSLVIKGARENNLQNVDVEIPLGRLVVVSGVSGSGKSSLINDI
                     LAKELLARLHRANTVPGRHDDIEGIKQLDKAIIIDQSPIGRTPRSNPATYTGLFTPIR
                     ELFASVPEAKLRGYSAGRFSFNVKGGRCENCAGDGIIKIEMHFLPDVYVPCEVCHGQR
                     YNREALEIHYKGKTIADVLSMTCEQALDFFGSIPAVARKLQTLVDVGLGYIRLGQPAT
                     TLSGGEAQRVKLATELSRRPTGRTLYILDEPTTGLHMEDVNKLLGVLHALVDAGNSMV
                     VIEHNLDVIKNADHLIDMGPEGGDAGGQVIATGTPEQVAKAEKSYTGQFLKKLL"
BASE COUNT          770 a         1186 c         1156 g          924 t
ORIGIN      
        1 cggactgatc tcgttaccct cgctgtagcg tgggtggatg ggcacttcgg cctctacctt
       61 ttcgttggcg cgtacggcat taaacactac gtggcgcagt tgatgtttct tggggtcgaa
      121 gtcggcggtt ttaataagcg ccgtaaaagt tttgtcaccg gcctgcaggt tgacagggtg
      181 atgcttgccc gcctgttgcc aaaccttcag tacggccatg tagtcaccct gcaccaccac
      241 cgaatccttg ccgtggtcgt ggatgaccgc cggcacagta ccttcacggc gcagctgccg
      301 tacctgcttt ccggtcactt tacgcggttc gagagtcaaa ctgatagtat cttgggccat
      361 actgaatata actccttttc agccacaaat tataacagat gctgtttctc ctgtcatctc
      421 gaccggagga acaacgcggt agggggatct cccgctgcgg ccgagatgac acgccaggaa
      481 cactggacgc tgatagccgg gcactgtacg ctataagggc atgtttgatg taagcggcat
      541 tatcgcagcc ggcggattgg cactgatagc cttgattatt tttgccgagg tcggcttgat
      601 ggtcggcttt ttcctgccgg gggacacgct gctcttaagc gccggcatct tcgccgccca
      661 gggcaaactg ccgatagagt ggacgatacc gctcgtagcc ttggccgcca tcctgggcga
      721 taacacctct tatttcatcg gccacaagct gggaccgcgc ctgttccgca aaaaagacgg
      781 actcatcttc cgccaggagt atgtggaaaa ggccgaaaag ttttatgaaa tatacggcaa
      841 taaaactgcc ctgctggcgc actggctgcc cgttatccgt acctttgcgc cgctgctggc
      901 cggcgtaggc aaaatgcgct accgtacctt tgccatttac gatgccatcg gcgatatcgg
      961 ctgggccgcc gccgtcacgc tactcggcta cttcattggc acgcgcatcc ccaatattga
     1021 ccactacatc atgctggccg tcgccgccgc cgtcgttatc tccttcggcc caaccgtcta
     1081 ccatctcata aaagccgtcc gcgaacgtcg ccgcacaccc gaatccgaat aagcaatgct
     1141 tgcaggcatt ctttaatcgt ctctgtctgc tgacgatatg ggcggacccg gcaagcttaa
     1201 aatacggcgg cattgcacgg gccggagccc tagcagcaat ggacccgggg gttgagccgg
     1261 actttactca agaattatct tggaagatat gaacacccgg gctagagcag tttcttaagg
     1321 aactggccgg tataggattt ttctgccttg gcgacttgtt ccggggtacc agtcgctatg
     1381 acttggccgc cggcgtcgcc gccttccgga cccatgtcta tgaggtggtc ggcgttcttt
     1441 ataacgtcca ggttgtgctc tatgacgacc atgctgttgc cggcatctac gagcgcatgc
     1501 agcactccga gcagtttatt cacgtcttcc atgtgcaggc cggtggttgg ctcgtccagt
     1561 atgtacagcg tacggccagt agggcgccga gacagctcgg tcgcaagctt aacacgttga
     1621 gcttccccgc cggacaaggt cgtagccggc tggccgagac ggatgtaccc aagacccaca
     1681 tccaccagcg tttgcagctt gcgggcaacg gccgggatgc tgccaaagaa gtccagcgcc
     1741 tgctcgcagg tcatgctgag cacgtcggca atggtcttgc ccttgtaatg gatttccagc
     1801 gcttcgcgat tgtagcgctg gccgtggcag acttcgcacg gtacgtatac atccggcaaa
     1861 aagtgcattt ctatttttat gatgccgtct ccggcgcagt tttcgcatct tccgccctta
     1921 acattgaaac tgaagcgccc ggcgctatag ccgcgcagct tggcttccgg cacgctggca
     1981 aacagctcgc ggatgggcgt aaacaacccg gtataggtgg ccggattgct tctgggcgtg
     2041 cggccgatgg ggctttggtc gatgatgata gccttgtcga gctgcttgat gccctcgatg
     2101 tcatcatgtc ggcccggtac ggtgttggcc cggtgcaaac gagccagcaa ttctttggcc
     2161 aaaatgtcat taatgaggct cgacttgccg gaaccggaca cgccgctcac taccaccagc
     2221 cggccgagcg ggatttccac gtccacattc tgcagattgt tttcccgggc gccttttata
     2281 accaaagatt taccgttgcc cggccggcgc ttcttgggca cggcgattgc tctgtgtccg
     2341 gctaagtact gggcggtaat gctctctttc acggcggcga cctcttccgg cgtgccgttg
     2401 gccacaactt tgccgccgtg aatgccggcg cccgggccga tgtccaatag atagtcggcc
     2461 gtccggatag tatcctcgtc gtgctccacc acgatgacgg tattgccgag atcgcgcagg
     2521 tgcttcagcg tacgaatgag ccgttcgttg tcgcgctggt gcaggcctat cgacggctcg
     2581 tcgagtacgt acagtacgcc ctgcagcccg gaaccaatct gcgtagccag gcggatacgc
     2641 tgcgcttccc cgcccgatag cgtagtggca ctgcgcagca gcgtcaggta gttgaggccc
     2701 acgtcctgca ggaactgcag acgggcgcag atttctttga gaatcaggtt ggcaatcttc
     2761 tgctctgtgg cattgagttt cagatgattg aaccaatcga tggcatcgtc gatgctaagc
     2821 gtgcagacat ccataatgga ctgtccgccg acggtgattg ccagggcttc gggcttcagc
     2881 cgcaggcctt tgcacatgtg gcagggcttt tcctgcatga agcgctcgat gtcgcggcgg
     2941 atgaaatcgc tgtcggtttc gcggtgtcgc cgctccaaat tcggcaccac gccctcatag
     3001 gtcgtttcaa agctgcggcc catgccgatc tgcacccgga acttttcgcc gccggtgccg
     3061 tacattatgc gctgcatgtt ttcggcactg atttcactcg tcggaacgtg caggctgaag
     3121 ccgtacttcc cggccaccgc ctccagcttt ttcatgtacc agttatcgac gttgatacgg
     3181 ttgtagggac ggatggcgcc ctcggcaatc gtcagccggc cgttggggat gaccagttcc
     3241 ggatcgactt ccagccggga gcccaagccg gtacacaccg ggcaggcccc gtgcgggctg
     3301 ttgaagctaa aggtgcgcgg ttcgagctcg ggaattacca catccggatg atccggacag
     3361 gcgtacatga gagagtacga agtaagctcg tcggtatccg cattcaggac gttcaacttg
     3421 ccttcggcca catccagggc ctgctccacc gattgtacga gccggctgcg gctttcctcg
     3481 ttgtttacca gccggtcggc cacgatttct atggtgtgtt tgtagttctt gtccagctgc
     3541 gggaactcat ccaacgcata taccacggcg tccacccgca cccgcgcaaa accggcgcgc
     3601 tggtattgtt cggggatgtg ttcaaaggcg ccctttttgt ccacgactac gggggccagt
     3661 atcatcagcc gggcgccttc cggcaaggcc gccacttgat cgacgatggc attggtggtc
     3721 tggcgctcca cgggattgcc gcagaccgga cagtgcgggg taccgatacg cgcgaacagc
     3781 aggcgcaggt aatcataaat ttcggtgaca gtcgccaccg tagagcgcgg gttgcggctg
     3841 gtggactttt ggtcaatgga aattgccggc gaaaggccgt ctatctggtc gacatccggc
     3901 ttttccatga gccccaggaa ctgccgggca tagctgctca agctctccac atagcggcgc
     3961 tgtccttcgg cataaatggt atcaaacgcc aggctggact tacctgaacc ggacaggccg
     4021 gttataacca ccagct
//
LOCUS       DASSFD010000121         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_244769, whole genome shotgun sequence.
ACCESSION   DASSFD010000121 DASSFD010000000
VERSION     DASSFD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244769"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /gene="rpsK"
                     /locus_tag="VFH39_02795"
     CDS             complement(<1..221)
                     /gene="rpsK"
                     /locus_tag="VFH39_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6924728.1"
                     /translation="MAESTTAAASSSKRKKAKRTVAYGQVHVLATFNNTIISFTDGNG
                     GVLTSSSAGACGFRGSKKGTAYAAQVAAER"
     gene            complement(239..625)
                     /gene="rpsM"
                     /locus_tag="VFH39_02800"
     CDS             complement(239..625)
                     /gene="rpsM"
                     /locus_tag="VFH39_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6924729.1"
                     /translation="MARISGVTIPNDKQIWVALTYVYGIGPTTSRSILEKAKQDPTVR
                     VKDLTDGEVSRIQEIINNELTVEGELQRIVSGNIKRLKDIKSYRGSRHNANLPSRGQR
                     TKTNARTRRGRKVTVGGTAKKVASKT"
     gene            complement(629..745)
                     /gene="rpmJ"
                     /locus_tag="VFH39_02805"
     CDS             complement(629..745)
                     /gene="rpmJ"
                     /locus_tag="VFH39_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET6924730.1"
                     /translation="MKVRASVKKISPDDKIVRRKGRLYVINKFKPKHKQRQG"
     gene            complement(877..1098)
                     /gene="infA"
                     /locus_tag="VFH39_02810"
     CDS             complement(877..1098)
                     /gene="infA"
                     /locus_tag="VFH39_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6924731.1"
                     /translation="MTANKEVIELTGTIVETLPGTQFRVELENGHQIIAHVAGKMRKH
                     FIRIVPGDSVTVELTPYDLSKGRITYRKS"
     gene            complement(1158..>2420)
                     /gene="secY"
                     /locus_tag="VFH39_02815"
     CDS             complement(1158..>2420)
                     /gene="secY"
                     /locus_tag="VFH39_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET6924732.1"
                     /translation="MIAGLGPYINASIIMQLLTKAIPQLEALHKEGEFGQKKINQYTR
                     ILTLPLAIIQSIGSIFLIRQFASQVGGVGDITAHSSILQWVLMVAALTGGSMLLMWLG
                     ELVTEQSVGNGISLIITVGIVSRLPANISSVASSVYNKKDHWKILGHNLPINERAFWY
                     GIILLVAVIFITWAVVMLNEAARNLTVNYAKRVQGNRAYGGITTVLPVKLITAGVIPI
                     IFAVAFLSVPTYLGQLLANNHSARLAHLGSNLYTWFQAPSAATFAQGGFIPYIYPVTY
                     FLLVFIFTFFYTSIAFNSKEIAENLQKQGGFLEGVRSGNQTEAYLSKVVNRLTLFGAI
                     SLGLLALLPIVAQVFISQNISVGGTSILILVSVALQTLRAVESRALMVTYDQYSQPDF
                     FYDSPSEEGGAVTGRRLRFVPRIGRNRK"
BASE COUNT          512 a          652 c          694 g          562 t
ORIGIN      
        1 cgctcggcgg cgacctgcgc ggcgtaggcg gtacccttct tggaaccgcg gaagccgcag
       61 gcgccggcgc tgctgctggt cagcacgcca ccattgccat cggtgaacga aatgatagtg
      121 ttattgaacg tcgccaagac gtgcacctgc ccgtaggcaa cggttcgctt agctttcttg
      181 cgcttgctgg acgatgcagc agcggtggtt gattctgcca tatgcttaca tccttccttt
      241 aggtcttact cgctactttc ttggcggtgc caccgacggt gaccttgcgg ccacggcggg
      301 tgcgggcgtt ggtcttggta cgctgaccgc ggctcggcag gttggcgttg tgacgcgatc
      361 cgcggtagga cttgatgtcc ttcagtcgct tgatgttgcc gctgacgata cgctgcagct
      421 caccttcgac ggtgagttca ttgttgatga tctcctggat acgactgact tcgccgtccg
      481 tcaggtcttt gacacgcacg gtcgggtcct gcttggcctt ctcgaggata ctgcggctgg
      541 tcgtgggacc gataccgtag acgtacgtca gggcgaccca gatctgcttg tcattcggaa
      601 tggtaactcc ggaaattcga gccatagact acccctgcct ctgcttgtgc ttcggcttaa
      661 atttgttgat aacgtaaaga cgccccttgc gacgtacgat cttgtcgtcg gggctgatct
      721 ttttgacact tgcacgcact ttcatgcgga tcgcgctcct ttcagagtga gacacactgc
      781 tattagttaa tgatttaagg tcgagtacct tcacaaagct tcgcatctgc gagtgcacca
      841 tggtgcccag gcgatgtgac gccttgtgaa agtaccctac gacttgcgat acgtgattct
      901 gcccttacta agatcgtaag gtgtcaactc taccgtaacg ctgtcacctg gcacgattcg
      961 gatgaaatgc ttccgcatct tgccggcgac gtgagctatg atttgatgac cgttctcgag
     1021 ctctacacga aactgagtcc ccggtagggt ctctacaatc gttcccgtca gctcgataac
     1081 ttccttattg gcggtcataa atataacaag gggtaattat agcagccaaa acaacacttt
     1141 gtaaagagtt ttcgccgtta cttacggttt cgcccgatac gcggtacgaa acgcaggcgt
     1201 cgtccggtga ccgcaccgcc ctcttccgat gggctgtcat agaagaaatc gggttgactg
     1261 tactggtcgt aggtgaccat gagggcccgt gattcgacgg cccgcagggt ctgcaacgca
     1321 accgatacaa gaatcaaaat gctagtaccg ccgacgctga tgttctgact gatgaacact
     1381 tgggcgacga tcggcaacag cgccagcagc cccaagctga tagcgccgaa gagcgttaac
     1441 cggttgacga ccttgctcag gtaagcttct gtctggttac cggagcggac gccttccagg
     1501 aagccgcctt gcttctgcag attttcggcg atctctttgc tgttgaaggc gatgctggtg
     1561 tagaagaacg tgaagatgaa caccagcaag aagtacgtta ccgggtagat gtacgggata
     1621 aagccgccct gagcgaaggt ggccgcgctg ggcgcctgga accaggtgta caggttggag
     1681 ccgaggtgcg ccaaccgggc actgtggtta ttggccagca gctgccccag ataggtcggg
     1741 acgctcagga acgcgaccgc gaaaatgatg gggatgacac cggcggtgat gagtttgacg
     1801 ggcagcacgg tggtaatgcc gccgtaggcc cggttaccct gtacccgctt ggcatagtta
     1861 acggtcagat tacgtgctgc ctcgttgagc atgacgaccg cccaggtaat gaatatgacc
     1921 gctactaaca aaatgatgcc gtaccagaag gcgcgctcgt tgatgggtag gttatgcccc
     1981 aaaattttcc agtgatcttt cttgttataa acggagctgg ccaccgacga aatgttggcc
     2041 ggcaaccggc tgacgatgcc gacggtgatg ataagcgaga taccgttacc cacactttgt
     2101 tctgttacca actcacccag ccacatcagc agcatcgaac cgccggtcaa ggcggccacc
     2161 atcaggaccc actgcagaat gctggagtga gcagtgatat ctccgacgcc cccgacttgc
     2221 gaagcgaact gtcgaatcag gaatatggag ccgatggact ggatgatagc caagggcagc
     2281 gtcaggatac gggtgtactg gttgattttc ttctggccga actcgccttc cttgtgcaag
     2341 gcctccagct gcggtatggc cttggtcagc agctgcataa taatcgaggc gttaatgtac
     2401 gggccaaggc cggcaatcat
//
LOCUS       DASSFD010000122         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1802137, whole genome shotgun sequence.
ACCESSION   DASSFD010000122 DASSFD010000000
VERSION     DASSFD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802137"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFH39_02820"
     CDS             <1..254
                     /locus_tag="VFH39_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET6924733.1"
                     /translation="GWKEMNTKEALAQGVLGAFGDKYGETVKVYTAGDPEGEWFSKEI
                     CGGPHVEHTGLLGEGGKKFKISKEESSSAGVRRIKAVLQ"
     gene            424..1758
                     /locus_tag="VFH39_02825"
     CDS             424..1758
                     /locus_tag="VFH39_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HET6924734.1"
                     /translation="MLEKFKTVAGSAAKKAAEGATKAAANVRTKAGKPAGDQYLVALD
                     IGTEFVKALIGRVQADGSIDIIGVGRRHQALTDMQAGAIADIAGVVDNCDKALSEAEA
                     QVGVSVRTAIIGIAGELVKGTTTTIRVARKNGEKPLDLAEMEKIINLVQSRAEAKAKQ
                     QLAWELGGKEVEVRLVNSALVSIEIDGYPVTSPIGFQGKQVVVQLYTAFAPMIHIGAL
                     ERTAQELDLDLLAVAAEPFAVARAMIGDKPDQNLSAILMDVGGGTTDIAVVNDGGVQG
                     TKMFGIGGRAYTRAIERDLGVEFDQAEELKLSLSTNKVPAQKRPAIEAALDKTADVWV
                     AGIEVALGEFTKLDHLPNHLFLCGGGSSLDLLMDRLENSQWYRGLPFTKRPTVHHIEP
                     EQVAGIVDKTGAVNDHTYITAMGLLRVGLDTMRFARSGGNSIRDKLDKMLSV"
     gene            1828..>3187
                     /locus_tag="VFH39_02830"
     CDS             1828..>3187
                     /locus_tag="VFH39_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924735.1"
                     /translation="MADKSKDVIYIDVDDEITGVIDKVRSSDGRVLALVLPKRASVFQ
                     SIVNMKLLKRAADEDKKRLVLITSEAGLLPLAGAVKIHVARTLQSKPEIPAAPDVSDT
                     EESVDEDEPLAGPQEVTAETAGNRPIGELAGPAAAVPPLTTDDAMETVELDNDTEEEA
                     ASLPVSTAPLPMAAKKDKKLKVPNFNRFRLLLALGALGLIIIALLFVAVTVLPKATIA
                     IKTNATNVNTNLSLTLDTTALALDETANVVPAKQVQEQKSFTQTASATGQANKGDRAS
                     GTVSMTAQACAPNIGTPDDVPAGTGVSYNGLTYITQSDASFSFSNASGSCVNYVSDGV
                     DIIAQGAGAKYNADNVSFAVAGRSDVKATGSASGGTDNIVKVISQADIDGAKGKISAQ
                     DGGAKHDLQSQLAQDGYYAVMATFNAGTPSVTTSANAGDTADSVTVTEAITYTLIGAH
                     KSD"
BASE COUNT          747 a          928 c          965 g          547 t
ORIGIN      
        1 tgggctggaa ggagatgaac accaaagagg ccttggcgca aggcgtactc ggggcgttcg
       61 gcgacaagta cggtgaaacg gtcaaggtat atactgccgg cgacccggag ggcgagtggt
      121 tcagcaagga aatctgcggc ggcccgcatg tggagcacac cggccttttg ggcgaaggcg
      181 gcaaaaagtt caaaatctct aaagaagaat cgtcttcggc gggcgtccgc cgcatcaaag
      241 ccgttttaca gtaattgata ccggctttca ctgttccggt tatgggctta tccggttccg
      301 tgagtcattg gaaactgaaa atcggatatt gaaaatttaa gctgcgcctc agctttaccc
      361 ggttactata gtgcaggtac cggcttccgg gtgcctcctg ccaatgatat acttataacg
      421 attatgcttg aaaaatttaa aaccgttgcc gggtctgctg ctaagaaggc cgctgagggt
      481 gcgaccaaag cggcggcgaa cgtcaggacc aaagccggta agccggccgg cgaccagtat
      541 ctggtggcgc tcgacattgg tacggagttc gtaaaagcgc tcattggccg cgtacaggca
      601 gacggcagta tagatatcat aggagtcggg cgtcgccatc aggcgcttac tgacatgcag
      661 gccggcgcca tcgccgacat cgccggcgtg gtcgacaact gcgacaaggc gctttcggag
      721 gccgaggccc aggtcggcgt cagcgtacgc accgccatca tcggcatcgc cggcgaactg
      781 gtaaagggca cgaccacgac cattcgcgtt gcccgcaaga acggcgagaa accactggat
      841 cttgccgaga tggagaagat tatcaacctg gtacagagcc gggccgaagc taaggccaag
      901 cagcagcttg cctgggagct cggcggcaaa gaagtcgaag tacggctggt gaacagtgcg
      961 ctcgtgagca tcgaaataga cggttacccg gttaccagcc ccatcggttt ccagggcaag
     1021 caagtggtag tgcagcttta cacggccttc gcgcctatga tccacattgg ggcgttagag
     1081 cgcaccgcgc aagagctgga tctcgacctg ctggccgtgg cggccgagcc gttcgccgtg
     1141 gcccgcgcca tgatcggcga caagccggac cagaacctca gcgccatttt gatggatgtc
     1201 ggcggcggca ccactgatat cgcggtcgtc aatgatggcg gcgtgcaggg caccaaaatg
     1261 tttggcatcg gcggccgcgc ttacacccgg gccatcgaac gcgaccttgg cgtagaattc
     1321 gaccaggccg aagagctcaa actaagctta agtacaaaca aagtgccggc gcagaaacgg
     1381 ccggccatag aagcggccct ggacaagacc gcggatgtat gggttgccgg catagaggtt
     1441 gccctgggcg agttcacgaa gctcgaccac ctgccgaacc acctcttcct gtgcggcggc
     1501 ggctccagcc tggacctgct gatggaccgt ctggaaaact cccagtggta tcgcggcctg
     1561 ccgtttacca aacgccccac ggtgcaccac atagagcctg aacaagtcgc cggtattgtt
     1621 gacaaaaccg gtgccgtcaa cgaccataca tacataaccg caatggggct gctccgggta
     1681 gggctggaca ctatgcggtt tgcccgctcc ggcgggaact ccatccgcga taaactggac
     1741 aagatgttaa gcgtatgacg gcaagaaacc agaagcatga gacaagcaac agggctgccc
     1801 tgcatcctga ctccgtagga gtttatcgtg gccgataaaa gcaaggacgt catttacata
     1861 gacgtcgacg acgagatcac cggcgttatc gacaaggtcc gcagttccga cggacgcgtg
     1921 ctggcactcg tgctgcccaa acgcgccagc gtgttccaga gcatcgtcaa catgaaattg
     1981 cttaaacgcg cggcggacga agacaagaaa cgcctggtgc ttataacgag cgaggcgggg
     2041 ctgttgccgc tggccggcgc cgtcaagata catgtggcga ggactttgca gagtaagccg
     2101 gagataccgg ctgcccccga tgtgtcggat accgaagaga gcgtggatga ggacgagccg
     2161 ctggccggcc cgcaggaagt caccgccgaa accgccggca accgtcccat cggtgagctg
     2221 gccggaccgg cagcagccgt gccgccgctg actactgacg atgccatgga gacagtggaa
     2281 ctggacaatg ataccgagga agaagctgcc agcctgccgg tcagcactgc gccgctgcct
     2341 atggccgcca aaaaagacaa gaagcttaag gtccctaact tcaaccggtt ccggctgttg
     2401 ctggcgctgg gcgcgcttgg gcttatcatc atcgcactgc tgttcgtggc cgtcacggtg
     2461 ctgcccaaag ccaccatcgc catcaaaacc aacgccacga acgtgaacac caacttgagc
     2521 ctgacgctgg atacaacggc actcgcgctg gacgagacgg cgaacgtggt gccggccaag
     2581 caggtgcagg agcaaaagag ctttacgcag accgcctcgg cgaccggtca ggccaataag
     2641 ggcgaccggg ctagcggtac ggtatcaatg acggcgcaag cctgtgcccc caacatcggt
     2701 actcctgatg atgtaccggc aggcacgggc gtcagctaca acgggctgac ctacattacc
     2761 caaagcgatg ccagtttcag cttcagtaat gcctcgggca gctgcgtgaa ctatgtatcc
     2821 gacggcgtgg atattattgc ccagggcgca ggcgcgaagt ataacgccga caacgtcagc
     2881 tttgcggttg ccggccgcag cgatgtaaaa gccaccggtt ccgccagcgg cggcaccgac
     2941 aacattgtca aggttatcag ccaggccgat atagacggtg ccaagggtaa gatcagcgcc
     3001 caggacggcg gcgccaaaca cgacctccag agtcaacttg cccaggacgg ctactacgcc
     3061 gtcatggcca ctttcaatgc cggtacgccc agcgtgacca ccagtgcgaa cgccggcgat
     3121 accgccgaca gcgtcacggt caccgaagcc attacctata ccctgatcgg tgcccacaaa
     3181 agcgatc
//
LOCUS       DASSFD010000123         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_797281, whole genome shotgun sequence.
ACCESSION   DASSFD010000123 DASSFD010000000
VERSION     DASSFD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797281"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1900
                     /locus_tag="VFH39_02835"
     CDS             236..1900
                     /locus_tag="VFH39_02835"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET6924736.1"
                     /translation="MKPPQHLLIMGRIVGWFTILYGLIILSGVLLRTGLFHPIHVINA
                     FFSSVQPLIGLSYVYLGTLLLRRKFNAWLLATCLSVFIIAGSVLQLSHVVTDDTLTAP
                     VGLWLHTILPLAMLALLVISRRIFTVRSDVRSFRQAVLTSVILLLITVVYGTGGFLVL
                     DRHDLGHEVNFGSALDQTIDQFGLTTARPVAHSRRARVFLDSLPVISVGALVYVAVSF
                     FQPLRVRLRHQAGQRQHVERLLWQYPSDIDDFFKLWPHDKRYYFSKSGESGLAYHVSR
                     GVALVVGDPFGQPKEFGRLLTSFLELCFVNDWLPAFIHVARSNKERFTKRGLSLQKIG
                     EEAIVRVDTFDSVAKGKYFRQIGNRFSRLGYTTSYLTPPHDAPTMHRLQEISAEWLRR
                     PGRAERGFMLGYFNDDYLQQCALFVAKDPDGVIQGFVNLVPTFEADTANYDLLRCSEA
                     APGNCNDFLLMNLLGQLQSDGISRFNLGLCPLAGLDTAKPDDNVINSALRFFYSNGDR
                     LYSFSGLRRFKSKYEPTWEPRYIAYPGGIRNFTRIITAFNRVSKIK"
     gene            complement(1946..2893)
                     /gene="rplA"
                     /locus_tag="VFH39_02840"
     CDS             complement(1946..2893)
                     /gene="rplA"
                     /locus_tag="VFH39_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET6924737.1"
                     /translation="MAKAPKNEEEIKTTADVPAEVEEQIVNAEAVEVAEEKVETSSAE
                     GKLAKSGKRSAKAIAEAEEKVAKEERKAHTGEAEAESKPKAPVKPARSRLERRGKKFR
                     DAAKQVEADKVYGLEEAVTLAAKTSTVKFDATVELHINLNVDPRHADQNIRDNLVLPA
                     GTGKTVRVAVFADDKVEGADLSGVETITKALDKGELNFDVLVATPANMPKLGKYARTL
                     GPRGLMPNPKSGTVTSDLTKAVQDAKAGRVEYRVDSTGIVHLGVGKVSFTPAQLTDNV
                     RAVLASVRANKPSSVKSNYIRAVHLTTTMGPSITVSTAE"
     gene            complement(3056..3202)
                     /locus_tag="VFH39_02845"
     CDS             complement(3056..3202)
                     /locus_tag="VFH39_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924738.1"
                     /translation="MHTIKLTTGSHSIVSAGVFLITPMKQTKVVNQPLIRKNRYLMHQ
                     LKSL"
     gene            complement(3217..3321)
                     /locus_tag="VFH39_02850"
     CDS             complement(3217..3321)
                     /locus_tag="VFH39_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924739.1"
                     /translation="MAEEKAMDMNTDDVEAVMKMVAGTARSMGVEVEG"
BASE COUNT          614 a          952 c          906 g          854 t
ORIGIN      
        1 gtcacatcga gactggcgtg cgcaacatgc ttcggattgt acggcacaca aacgatatgg
       61 tcatcggcga tggcctgccg gatctgctgg ttactataaa cgctcatctt ccccctcttt
      121 tagcccaccg ccttgtttga atatatttta cgattatgtt cttgtgttca caagcggtaa
      181 ggcacaattt tacaaccccc gctcgccact gctccatagg gtacaattag atgaaatgaa
      241 gcctccacag cacctcctta taatgggccg gatcgtcggc tggttcacca tactgtacgg
      301 cctgatcatt ttgtccggcg tattgctgcg gacagggctg ttccatccga tacatgtcat
      361 caacgccttt ttctcgagtg tccagccgct catcggcctc agttacgtct atttgggaac
      421 gctgcttttg cggcggaagt tcaacgcctg gctcctggcc acctgcctgt cggtgttcat
      481 catcgccggc agcgtgctgc agctgtccca tgtcgtcacc gacgacacgc tgacggcacc
      541 ggtcggtttg tggctgcaca ccatcttgcc cctggccatg ctcgccctgt tggtcataag
      601 ccggcgtata ttcacggtca gaagtgacgt gcgcagtttc cgtcaggcag tattgaccag
      661 tgtcatcctg ctgctcatta cggtggtcta tggcacgggt ggctttttgg tgctcgaccg
      721 gcatgacctg ggtcatgaag tcaactttgg ctccgccctg gaccagacta tcgatcagtt
      781 tggacttacc actgcgcggc cggtagccca tagccggcgg gcccgcgtgt ttttggattc
      841 gctgcctgtt ataagtgtcg gcgcgctagt gtacgtggcc gtgtcgttct tccagccgct
      901 gcgcgtgcgg ctgcgacatc aggctggtca gcggcagcac gtcgaacgac tgctctggca
      961 ataccccagt gatatcgatg atttcttcaa gctgtggccg cacgacaagc gctactactt
     1021 cagtaaaagt ggggagtccg gcctggccta ccatgtcagc cgcggggtcg ccttggtggt
     1081 cggcgatccc ttcggccagc ccaaggaatt cggtcggctg ctgacgtcat tccttgagct
     1141 ttgtttcgtc aatgactggc taccggcctt cattcatgtc gcgagaagca ataaggaacg
     1201 attcaccaag cgaggcttaa gtctgcagaa aatcggcgaa gaagcgattg tgcgtgtaga
     1261 tacgttcgat tcggtagcca aaggtaaata cttccgccag ataggaaacc gtttcagccg
     1321 gttaggctat acgaccagct atctgacgcc gccgcatgac gccccaacaa tgcaccgctt
     1381 acaagaaata tccgccgaat ggctgagacg tccgggacgt gccgaacgcg gctttatgct
     1441 cggatatttc aacgacgatt atctgcaaca atgcgcattg ttcgtcgcca aagaccccga
     1501 cggcgtaatc caggggttcg tcaacctcgt accgaccttt gaagccgaca ctgccaatta
     1561 cgatctgttg cgttgtagcg aagctgcgcc aggcaactgc aacgattttc tgctcatgaa
     1621 cctgcttggt cagcttcaat cagatggcat tagccggttc aacctcggcc tgtgtccgct
     1681 ggcaggtctc gatacagcaa agccagatga caatgttata aacagtgccc tgcggttctt
     1741 ttattcgaac ggcgaccggc tgtattcgtt cagcgggctg cggcgcttta aatccaagta
     1801 tgaaccgact tgggagccgc ggtatatagc ttacccgggc ggcatccgta acttcacccg
     1861 tatcatcacc gccttcaacc gtgtttcgaa aataaaataa gcgccccttt ttggagaggc
     1921 gcttacatat ccggctatga acggcttact cggcggtcga gaccgtgatg ctcggaccca
     1981 tagtggtcgt caggtgaacg gcacggatgt agttgctctt gacgctagaa ggcttgttgg
     2041 ccctgacgga ggccaggact gcccggacgt tgtccgtcag ctgggctggt gtaaagctga
     2101 ccttgccgac gccgaggtgc acgataccgg tgctgtccac acggtattcg acacgaccgg
     2161 ctttggcatc ctgcacagcc ttggtcaagt cactcgtaac ggtaccgctc ttggggttgg
     2221 gcatgagccc gcgcggaccc aaggtacgtg cgtacttgcc gagcttcggc atgttggcag
     2281 gggtggcaac gaggacgtcg aagttcagct cccccttatc caatgctttg gtgatggttt
     2341 cgacgccgct caggtcagcg ccctcgacct tgtcgtcggc gaagacggct acgcggacgg
     2401 tcttaccggt accggcgggc agtaccaggt tgtcgcgaat gttctgatcg gcgtgacgcg
     2461 ggtcgacgtt caggttgatg tgcagttcta cggtggcgtc gaacttgacg gtgctggtct
     2521 tagccgccag ggtaaccgct tcttccaggc cgtacacttt gtcggcttca acctgcttgg
     2581 cggcatcgcg gaacttcttg ccgcggcgct ccagacggct gcgggccggt tttaccggag
     2641 ccttaggctt gctttcggcc tcagcctcac cggtgtgcgc tttgcgctcc tccttggcaa
     2701 ctttctcttc tgcttcggca atcgctttgg cgctgcgctt gccggacttg gccagcttgc
     2761 cttcggcact gctggtttcc accttttctt ccgcgacttc taccgcctcg gcgttaacga
     2821 tttgctcctc gacctcagcc ggtacatcgg cagtcgtctt gatctcttct tcgttcttcg
     2881 gggcttttgc catgataatt actcctttcg tggtgctaac ggctaataag cctcccacaa
     2941 ttactattca aatgctcttt tgtttacccc tgagtgccgg tggcgtcgcg ataattacct
     3001 agcggtacat tatcggttcc aaattgtctg tcggcgacta tttctcttac gctgttcaga
     3061 gcgacttcag ctgatgcatc aggtagcggt tcttgcgaat gagcggctgg ttcaccactt
     3121 ttgtctgttt catcggcgtt ataaggaaca ctccagccga aacgatgctg tgggagccgg
     3181 tcgtaagctt tatggtatgc atcagctggc atatgcttag ccttcgactt ccacgcccat
     3241 ggagcgggcg gtgccggcga ccatcttcat gaccgcttcg acatcgtcgg tattcatgtc
     3301 catggctttt tcttcggcaa ttttgc
//
LOCUS       DASSFD010000124         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1150558, whole genome shotgun sequence.
ACCESSION   DASSFD010000124 DASSFD010000000
VERSION     DASSFD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150558"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFH39_02855"
     CDS             complement(<1..378)
                     /locus_tag="VFH39_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET6924740.1"
                     /translation="MDNYKRLNYYRLPVTEVLEEVRSRETGLKPAEARDRLEHMGANA
                     LHRASHESAFTTFLRQFKNMLVAILLLSAGISLYLRDGRTAAILFAIALINTLVGFFQ
                     EHNAETLFASLQQLMVPKAKVLRA"
     gene            475..1683
                     /locus_tag="VFH39_02860"
     CDS             475..1683
                     /locus_tag="VFH39_02860"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6924741.1"
                     /translation="MTELNTSHYDSSELVPPHGADIWETQAAYDGNHEIDLAPDSLDE
                     VLAGGADSFGLYLTEIGRVPLLEGRPEEIEVAKRIEAGLHAARLIELAQVEDLDAAIG
                     NLREELISKTVPRFKPLQPLDDMLEDLGKSARKIRELPSGEQGDMLADLREVEQDGIA
                     ARNQFWAANLRLVVKYAKRKQNKGLTLQELVQAGNINGLLRAVQKFDYTHGEKFSTYA
                     TNWILQSLDRAIANTARSIRVSMPQRERINKMKRIQRDEMQILGRELTDAELAERLEV
                     TVAKIIELKQLDKELTSLNKTSEVTDSDTELGDLVDIDEKYVNVQAATNEVAMRTDVM
                     KMVGLLPQEQAALAVLRYGLNGRRPHTLKEAADELGISVDKVKHLDTKTNFNLRELAD
                     SLGLRDYLAD"
     gene            1744..2850
                     /gene="sigB"
                     /locus_tag="VFH39_02865"
     CDS             1744..2850
                     /gene="sigB"
                     /locus_tag="VFH39_02865"
                     /inference="COORDINATES: protein motif:HMM:NF005920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HET6924742.1"
                     /translation="MTSAAFESFEPIGDPLHPEAPVEDGVGQECYVELVDPSPVAQEY
                     DFEESPENGELDYSTDPDEVVATRDLVRQYLNDIGKVALLTAVEEVELSQRVEAGLYA
                     QHLIDNNQGFMDASKRELRALAVDGERAKDHLLQANLRLVVSLAKRYTGHGMPFLDLI
                     QEGNLGLIRAVEKFDYTKGFKFSTYATWWIRQNITRAMADQARTIRLPVHLVEQVNKA
                     RRIQREMSQELGRDVTIPELAAVLEITEARAQELLDYDRDPFSLDQSVGDSDEASLGD
                     FIEDQSSTGEAEQSAESALLAAHLRSILATFSDREAEIIRLRYGMDGREKKTLDDIGK
                     VFGVTRERIRQIEVRVMKKLREPDVKAALRGYLD"
     gene            complement(3153..3338)
                     /locus_tag="VFH39_02870"
     CDS             complement(3153..3338)
                     /locus_tag="VFH39_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924743.1"
                     /translation="MVGGTTGDESAGVAGVVSGVVDAEEITTVLGLETSVDVGECGEL
                     YGVVTLGKSGLELYVGT"
     gene            3340..>3921
                     /locus_tag="VFH39_02875"
     CDS             3340..>3921
                     /locus_tag="VFH39_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6924744.1"
                     /translation="MRNESWKIAQDMIVGFDAGTPKSVIRQVVADTHIGGIFLTGTTD
                     AVADGFTPRFYHRLSKVAGAPMLVASDEEGGIIHRLAYNFNFPNAAEMGRMSPRQVRH
                     IGMLVGEQMRADGVYMDLAPVLDINRGADQVIDYYGRSFGSDTTEIAAHAQAFMNGLH
                     AAGVYATLKHLVGLTTAVKNNRGVILDNSDAHKV"
BASE COUNT          965 a         1015 c         1150 g          791 t
ORIGIN      
        1 ggcgcgcagt actttggcct tcggcaccat cagctgctgc aggctggcga acagcgtctc
       61 ggcgttatgc tcctgaaaga agccgaccaa cgtgttgatg agcgcgatgg caaagaggat
      121 agcggccgtg cggccatcac gcaggtacaa cgatatgccg gcgctcaaca gcaaaatagc
      181 gaccagcatg ttcttgaact gccgcaaaaa cgtggtgaat gcgctctcgt gagaggcgcg
      241 gtgcagggca ttagccccca tgtgctccag acggtcacgg gcttcggctg gtttgaggcc
      301 ggtctcgcgg ctacggactt cttcgagcac ttcggtcacg ggcagccggt agtagttaag
      361 ccgcttatag ttatccattg ttgttcatta tacgggaaag aacggattac gctcttgctt
      421 gcaaaaacca gtaataggta tagaatattc acactaaagg acggacgtag atagatgaca
      481 gagttaaaca cgtctcacta tgattcaagt gagctggtac ctccgcacgg agctgatatt
      541 tgggagacgc aagccgctta tgacgggaat catgaaattg acctggcccc cgactctctg
      601 gatgaggtgt tggcaggggg tgccgacagc tttggactct atctgacgga aatagggcga
      661 gtgccattac tcgagggcag gccggaagaa atcgaggttg ccaagcggat agaagcaggc
      721 ctgcacgcag cacggcttat agagctggcg caagttgagg acttggatgc cgccatcgga
      781 aacctgcgcg aagaacttat ttccaaaacg gttcccaggt tcaagccgtt gcaacctctt
      841 gatgacatgc tcgaagatct gggcaaatca gcacgtaaaa tccgggagct accaagtggt
      901 gagcagggtg acatgctcgc agatctgagg gaagttgagc aggacggcat tgccgctcgc
      961 aatcaattct gggccgcgaa tctccggctg gtcgtgaagt acgctaaacg aaagcagaac
     1021 aagggtctga cactgcagga gctggtgcaa gcgggcaata tcaacggcct gctacgcgcc
     1081 gttcagaaat ttgactacac gcatggcgaa aaattcagta cctatgccac taactggata
     1141 ctacaatcgc tagatcgggc gatagccaac acggcacgga gtatccgggt gtccatgcca
     1201 caacgagaac gcatcaataa aatgaaaaga atccaaaggg atgagatgca aatcttgggc
     1261 agggaactaa cagatgccga gctggctgag cgcctggagg tcacggtcgc aaagatcatt
     1321 gagcttaagc agctggacaa ggagctgacg agcctgaata aaacatcaga agtgaccgac
     1381 agcgacacag agctcgggga cttggtggat attgacgaga agtacgtgaa tgtccaggca
     1441 gccacaaacg aagtcgctat gcggactgat gtaatgaaaa tggtcgggct gttaccccag
     1501 gagcaggctg cattggctgt gctccgctac ggcctgaacg gccgccggcc ccatacactg
     1561 aaagaggcag ctgacgagct tggtatttcg gtcgacaaag taaagcactt ggacacaaaa
     1621 accaatttca atctgcgtga actggccgat agcttggggc tacgcgatta cttggctgac
     1681 taataatctt aagttgcaaa tgtacatgga cgacgacaca ataggaccgg tctggaagaa
     1741 ataatgacta gcgcagcttt tgaatcattt gaacccattg gagacccgtt gcaccctgag
     1801 gccccggtag aggatggtgt gggtcaggaa tgctatgtgg agctggttga cccttcgccc
     1861 gtagcgcaag aatatgactt tgaagaatcg cctgagaatg gcgagctcga ctatagcacc
     1921 gatcccgacg aagtcgtggc cacacgcgac ttggtgcggc aatacctgaa cgatatcggt
     1981 aaagtcgcgc tgctgacagc ggtcgaggaa gtagagttgt ctcagcgtgt cgaggccggc
     2041 ctgtacgcgc aacaccttat agacaacaac caaggcttta tggacgccag caaacgggaa
     2101 ctacgggcgc tggcggttga cggcgaacgg gccaaggacc atttgctgca ggccaacttg
     2161 cgcctggttg tatcgctggc caaacgctac acgggtcacg gcatgccatt cctggatctt
     2221 attcaggaag gcaatctcgg gcttatccgc gcggtcgaga agttcgacta caccaaaggc
     2281 ttcaagttca gcacctacgc cacctggtgg atacgccaga acatcacacg cgcgatggcc
     2341 gaccaagctc gaaccatccg tttgccggtt caccttgtag agcaagtgaa taaagcacgg
     2401 cgtatacagc gcgaaatgag ccaggagctg gggcgggacg taaccatacc ggagcttgcg
     2461 gccgtactgg aaataaccga agccagagcc caggaactgc ttgattatga ccgggatcca
     2521 ttcagcctgg atcagagtgt gggcgacagc gacgaagcat cgttgggtga tttcatcgaa
     2581 gaccagagtt ccaccggcga agccgagcaa agtgcggagt ctgctcttct ggctgctcat
     2641 ttgcggagca tacttgctac cttcagcgac cgtgaagcag agattattcg cctgcgttac
     2701 ggcatggacg ggcgcgaaaa gaaaactctc gatgacatcg gtaaggtatt cggagttacg
     2761 cgtgagcgta tccgccaaat tgaggtcaga gtaatgaaaa agctgcgcga gccggacgta
     2821 aaagcggcct tgcgcggtta tctcgactaa ttgccggcca gtaacactat gtccgtgtga
     2881 cgctcatgct tgcacttttt tgctcacgag tgcagaatat cgtgattaac ggagagagcg
     2941 ggtacattta tgtctcaaca tggtccggaa gacgaagggt ggagtgaatc tcggcattat
     3001 ggtgagccag agcccagtca tgatcgtcgt ctgtggctag cccttgctgc ggccggtgtg
     3061 gtcgttctgg gtgctggcac ggcaggtgct attgcgtgga gcgagggcac cggccacact
     3121 gcggcagcgc cacctccgag tcactcgccc gatcatgtgc cgacatacag ctcaagcccg
     3181 gacttcccga gcgttaccac accgtatagc tcgccgcatt cgccgacgtc cacggaggtt
     3241 tccagcccga gcacggtcgt gatttcttcg gcgtcgacca cgcccgaaac gacaccggcg
     3301 acaccagcgg attcgtcgcc ggtcgtaccg ccaaccattg tgcgtaatga atcatggaaa
     3361 atcgcgcagg acatgatcgt gggctttgat gccggtacac cgaagtcggt tatccgtcag
     3421 gtggtggccg acacgcacat cggcggcata tttttgaccg gtaccaccga tgctgtcgcc
     3481 gacggtttta cgccccgttt ttatcaccgc ttgagcaaag tggcgggcgc accgatgctg
     3541 gttgccagtg atgaagaggg cggcatcatt caccgcctgg cgtataactt caactttcca
     3601 aacgccgctg aaatgggcag aatgtcgcca cggcaggtgc gtcacatcgg catgctggta
     3661 ggtgagcaaa tgcgcgccga cggcgtgtac atggacctgg cgccggtgct ggacatcaat
     3721 cgtggcgccg atcaggtcat tgattactac gggcgctcct tcggtagtga tactactgag
     3781 attgctgcac acgcccaagc ctttatgaac ggcctgcatg ctgccggcgt atatgccacg
     3841 ctgaagcatt tggtcggtct gacgaccgcc gtcaaaaaca acaggggcgt gatactcgat
     3901 aactccgatg cccacaaagt c
//
LOCUS       DASSFD010000125         5772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1454841, whole genome shotgun sequence.
ACCESSION   DASSFD010000125 DASSFD010000000
VERSION     DASSFD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454841"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFH39_02880"
     CDS             <1..298
                     /locus_tag="VFH39_02880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6924745.1"
                     /translation="ADEIISEDTYIFEPDSYAVAAHLERSMMQIAISQLILDSKLAQY
                     ASRFRAMSASHQRADESKQDLHLAFNRARRNIKDERLKESINGMKKTKRAEATV"
     gene            298..1602
                     /locus_tag="VFH39_02885"
     CDS             298..1602
                     /locus_tag="VFH39_02885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6924746.1"
                     /translation="MAGSIVSVKDLVVRIQFDEDPPDVNELVQVQNEHGTELLVDHLE
                     AGGIAFCLNVRTDRNLMKGMLADRTHRGIEIPTGPLTIGRILNALGDPLDGQPAVAGE
                     NLKKKDILKLPQRSTDFKIQKPEILETGIKVIDFFTPFVKGRKIGIIGGAGVGKTVLT
                     MELINNIARSGSGLSFFSGIGERIREGHELWDTLRENDLLKNTVMFFAQMNENPVQRS
                     LIGISATAAAEYFRDEEHKDILFFADNMFRYVQARNELATILDQIPNEGGYEPTIFSD
                     VKVLQDRLSSNENGSITSVQTIYVPADDISDPAVQFIQHEMDSIIVLSRKVAEQGIRP
                     AVDLTLTTSSLLTPEVVGDRHYVLSVQVQALLQKYESLKGIIAIIGENELSPADRADY
                     SKAKKLIQNFTQNMNVMTKHNGIPGDFFSRDETLKSIEEIIV"
     gene            1602..1928
                     /locus_tag="VFH39_02890"
     CDS             1602..1928
                     /locus_tag="VFH39_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924747.1"
                     /translation="MATRQDASTGEIVSDKESLASVASADSTTTMNVKVYSPFKVYYD
                     GEAASVSAESATGPFDILPRHHNFITLLVPCQLVIRDGKDEQTFDIGGGVMHVKANDV
                     VVFLDV"
     gene            complement(2153..3133)
                     /locus_tag="VFH39_02895"
     CDS             complement(2153..3133)
                     /locus_tag="VFH39_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924748.1"
                     /translation="MENSENKESASLEGESLEGSAPSAASSDQAPGTPAAGATPPSGE
                     KPKKKRGGIFEKFSVYLLLFMVIIIIAIMASVASYLNSRSAKPTTIQSQNLTQSALSQ
                     LATSDVTVGDAKQVLTVQSNAVFAGQVLVRGDLQVAGKLQLGGGLNISSLQVSGAAVM
                     GSGQVNQNLNVQGDTALAGQLTVQKSLNVNGNGTFNGNVSATQLTAASLQLSGDLNLT
                     HHIAAGGPNPGRSNGSALGGGGTSSVSGSDTAGSITINTGSGPGAGCFITVNFAQHFN
                     TTPHVVITPVGAAAGDLHYYVNRSTSSFSVCTTNPAPSGQTFGFDYIIFD"
     gene            3256..4002
                     /locus_tag="VFH39_02900"
     CDS             3256..4002
                     /locus_tag="VFH39_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924749.1"
                     /translation="MRLLKYKKYANIFLLGLLIACGSGHAAYSLQSSSSSYGVDEAFF
                     GNGGQFNACSSTYCSNQTAGELGVGNSKSDSYQVQAGSDITDRAPYLEFIVNNSTVDL
                     GTLTTTTTATATATFSVKTYLAGGYTVVNASPGPQNGGYTLHTLSTPTGSSIGNEQFG
                     MNLVANTNPASFGAGPQQAPDSTFGFGQVASGYDTPNLYKYVNGDTIAYSNSSSGETD
                     YTISYIYNISNLTPGGFYTLYHDLVATSTY"
     gene            4284..5501
                     /locus_tag="VFH39_02905"
     CDS             4284..5501
                     /locus_tag="VFH39_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924750.1"
                     /translation="MSNATPGAAATYTVTFKPASTTAIDGVILEFCDNDPIIADTCNA
                     PTGLNVGTPTVAFTSHVAGNPVVTTALPGTWGTPAAATAHTLKFATSSGDSGALDPAT
                     LYSFDVTTMTNPTATTGQTFYARLITYTANGAGSDYANYTATNPGDTTALDYGGFALS
                     TSQNVNITTRVQETLTFCTSGTFNTANDSTQLLDPMDGTNTAINANGQTGGSDCTAAT
                     TPDLTIGHGSPTAVIDNSQVDASLAFMQASTNATNGMTIRMKATNTCTNGGLSSTGGA
                     NCSINGVGGTTPATGVPGTTFFGLFVSPSTTTSGVATSTGTLVPDPNYNGGACTIALV
                     NTTCKFGMDDSATSGVISTYGDVIASSAVADGGTGPISQLNSKLLFGATAGLTVPAGI
                     YTGSESLIATGTF"
     gene            complement(5581..>5772)
                     /locus_tag="VFH39_02910"
     CDS             complement(5581..>5772)
                     /locus_tag="VFH39_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924751.1"
                     /translation="AVSGTLAATSKAMARESARTRPKFSNALFIFIFAPVFYIVLDYT
                     TIYYHANVWLANKWLYILH"
BASE COUNT         1283 a         1745 c         1592 g         1152 t
ORIGIN      
        1 agccgacgaa atcatctccg aggacaccta catcttcgag ccggacagtt acgccgtcgc
       61 cgcgcacctg gagcgttcga tgatgcagat tgccatcagc cagctgattt tggacagcaa
      121 actggcccag tacgcttccc gtttccgcgc catgagtgcc agccaccagc gggccgatga
      181 gagcaagcag gatctgcacc tggctttcaa ccgggcccgc cgcaacatca aggacgaacg
      241 cctcaaggag agtatcaacg gcatgaagaa gaccaagcgc gcggaggcga ccgtataatg
      301 gccggctcga tcgtcagtgt caaagacctg gtggtgcgta tccagttcga cgaggatccg
      361 cccgatgtca acgaactggt tcaggtacag aacgagcacg gcaccgagct gctggtcgac
      421 cacctggaag ccggcggcat cgccttctgc ctgaacgtcc ggaccgatcg taacctcatg
      481 aagggtatgc tggccgaccg tacgcaccgc ggcatcgaaa tcccgaccgg tcccctgacc
      541 atcggccgca tcttgaacgc gctcggcgac ccgctcgacg ggcaaccggc cgtcgccggt
      601 gagaatctca agaagaagga tattctcaag ctgccgcagc gcagcaccga cttcaagatt
      661 cagaagccgg aaatcctgga aaccggcatc aaggtcatcg acttcttcac cccgttcgtc
      721 aaaggccgca agatcggcat catcggtggt gccggtgtcg gtaagacggt gctcaccatg
      781 gagctcatca acaacatcgc ccgtagcggc tccggcttgt cgttcttctc cggtatcggt
      841 gagcgtatcc gtgaggggca cgaactatgg gataccctgc gcgagaacga cctgctcaag
      901 aacacggtaa tgttcttcgc gcagatgaat gagaacccgg tgcagcgttc gctgatcggc
      961 atctcggcga cggccgctgc cgagtatttc cgcgacgaag agcacaaaga catcctgttc
     1021 ttcgccgaca acatgttccg ctatgtccag gcgcggaacg agctggcgac catcctcgac
     1081 cagataccga atgaaggcgg ctacgagccg accattttca gtgacgtaaa agtgctgcag
     1141 gaccgtctga gctctaacga gaacggttcc atcaccagcg tgcagaccat ctacgtgccg
     1201 gccgacgaca tttccgaccc ggccgtgcag ttcattcagc acgaaatgga cagcatcatc
     1261 gtcctgtccc gtaaggtggc ggagcagggt atccgcccgg ccgtcgacct cacgctgacc
     1321 acgtccagct tgctgacgcc cgaagtcgtc ggcgaccgcc actacgtgct gagcgtgcag
     1381 gtgcaggcat tgctgcagaa gtatgagagc ctcaagggca tcatcgccat catcggcgag
     1441 aacgaacttt ccccggccga ccgggccgat tatagcaagg ccaagaagct cattcagaac
     1501 ttcactcaga acatgaacgt catgaccaag cacaacggta tcccgggtga cttcttcagt
     1561 cgcgacgaaa cactgaaaag tatcgaggaa atcatcgtct gatggctacc cggcaggatg
     1621 ccagcactgg cgaaatagtc agcgacaaag agtcgctggc cagtgtggcc agtgccgact
     1681 cgacgacgac catgaacgtc aaggtctact cgccgttcaa ggtctactat gacggcgagg
     1741 ctgccagcgt cagcgccgaa agcgccaccg ggccgttcga tattctgccg cggcaccata
     1801 acttcatcac cttgctcgtg ccgtgccagc tggttatccg cgacggcaag gacgagcaga
     1861 cattcgacat aggcgggggt gtgatgcatg tcaaggccaa tgacgtcgtc gtgttcctgg
     1921 acgtctaagg cggccgctat gcccggcaca tttcaggcgc tgtgctgcag tccgcagtca
     1981 ccgtaccttg tagtacggct cgcttacggg cttcgctcat catcctgaac tgcactcagg
     2041 caaagcggcc gcacacagtg acggtgccag aggtgaagta cagggtagta tccgatactg
     2101 ctgcaggggt aaagcccgtc ctagggcctg tacatgcgtc aggcggccat tatcagtcga
     2161 aaatgatgta gtcgaacccg aaggtctgcc cggatggggc agggttggtg gtgcatacgc
     2221 tgaagctgct ggtactgcgg ttgacataat aatgcaggtc gccggcggcg gcgccgacgg
     2281 gcgtgattac cacatgcggt gtggtgttga agtgctgcgc gaagttgacg gttatgaagc
     2341 agccggcgcc cggcccgcta ccggtattga tggtaatgct gccggccgtg tcggagccgc
     2401 ttacggacga cgtaccgccg ccgcccaggg cactgccgtt actacgtccc gggttggggc
     2461 cgccggctgc gatgtggtgc gtcaggttca ggtcaccact taactgcagg ctggcggctg
     2521 tcagctgcgt cgcactgacg ttgccgttga acgtaccgtt accgttcaca ttcagactct
     2581 tctggacggt cagttgaccg gccaaagccg tgtcgccctg cacgttcagg ttctgattga
     2641 cctggccgct gcccatcacg gcggcaccgg acacttgcaa cgaactgata ttcaggccgc
     2701 cgccgagctg taatttgcca gccacttgca ggtcgccgcg caccagcacc tggccggcga
     2761 agacggcatt gctctgcacg gtcaacacct gtttggcgtc gccgacggtc acatcgctgg
     2821 tggccagctg gctgagggcg ctctgggtaa ggttctgaga ttggatggtg gtcggttttg
     2881 ctgaccggct attgaggtac gacgcgaccg aggccatgat ggcgatgata atgatgacca
     2941 tgaacagcag caggtagacg ctgaacttct cgaatattcc gccccgcttc tttttgggct
     3001 tttccccgct aggtggcgtg gcgcccgcgg ccggcgttcc cggtgcctgg tctgaactag
     3061 ctgctgacgg agcgcttcct tcgagcgact cgccttccag gctggccgat tctttgtttt
     3121 cggaattttc catgttggtc cacgcttatg cttacccctt atcatacacg gtccacctac
     3181 aaaccggaaa cgccttgacg ggacgccagg gtagattgca tttcacaacg attttcggca
     3241 caatattgct tacgcatgag gctcctcaaa tacaaaaagt acgccaatat ttttctgctc
     3301 ggcttactta tagcttgcgg cagcggccac gccgcctatt cgctgcagtc gtcttccagt
     3361 tcctatggtg tcgacgaggc gttctttggt aacggtgggc agttcaacgc ctgctccagc
     3421 acctactgct ccaaccagac cgccggcgaa ttgggtgtcg gcaacagtaa aagtgacagc
     3481 taccaggtcc aggccggtag tgacataacc gaccgtgcgc catacctgga atttatcgtc
     3541 aataactcga cggtcgatct agggacgctt acgaccacca ccacggcgac ggcgaccgcc
     3601 accttctcgg tcaaaaccta tctggccggt ggctataccg tggtcaacgc ctcaccgggt
     3661 ccgcagaacg gcggctatac gctacatacg ctcagcaccc ctacgggcag cagtatcggc
     3721 aatgagcagt tcggcatgaa cttggtggcc aacaccaacc ccgcgtcttt cggagcgggt
     3781 ccccagcagg caccggacag tacctttgga ttcggccagg tggccagcgg ctacgatacg
     3841 ccgaacctct acaaatatgt gaacggcgat accatcgctt acagcaatag cagctccggc
     3901 gaaacggact acaccatcag ctatatctat aacatcagta acctcacccc gggcggcttc
     3961 tatacccttt atcacgacct ggtggctacc agtacctatt aaatcgttcc aatatttaca
     4021 gcagataagg cggtttgtcc ttggcaggct cgacacgtga gtgataggat tgccttgtaa
     4081 ctataaaaac aaaagcaaaa acaaaaacaa tcaggagcta aaaaaacgtt atgagcagga
     4141 atataaaacg tttggtcgag cgggccgcgg cggtattttc cgtcctggct ttaggatctg
     4201 gcatgacaat tgcgtcgctg ctctctccag gaggagcggg cgctctaccg acgggaggac
     4261 aggttcagac ccgttctatc tatatgagca acgccacacc tggggcagcg gcgacctata
     4321 cggtaacctt caaaccggca agcacaacgg ctatcgacgg tgtgattctt gaattctgcg
     4381 acaatgaccc gatcattgcc gacacttgta acgcaccgac gggcctcaat gtaggtacgc
     4441 cgacggtggc atttaccagt catgtggcag gcaacccggt agtcacgact gcactacccg
     4501 gcacgtgggg tacgcccgca gcggccacgg cccatactct gaaattcgca acttcttcgg
     4561 gcgacagcgg tgcattagac cccgccacgc tctacagctt cgacgttact actatgacca
     4621 accccaccgc gaccaccggc cagaccttct atgcccgcct cattacgtac acagccaatg
     4681 gtgccggtag cgactacgcc aactacaccg cgaccaaccc tggcgatacg acagcactcg
     4741 actacggtgg cttcgcactt tccacttctc agaacgtcaa cataacgacc agggtccagg
     4801 aaacactcac gttctgcact tccggtacgt tcaataccgc gaacgattcc acacagctcc
     4861 ttgaccccat ggatggaacg aacacggcca tcaatgccaa cggccagacc ggtgggtcgg
     4921 actgtacggc agccaccaca cctgacctga ctatcggaca cggttcgccc accgccgtca
     4981 tcgacaactc tcaggtagac gcgtcgctag cctttatgca ggcttccacg aacgccacga
     5041 acggtatgac catccgcatg aaagctacca acacatgtac gaacggcggc ctgagctcga
     5101 ctggcggcgc caactgttcc atcaacggtg taggcggtac cacgcctgca accggtgtgc
     5161 cgggtaccac tttcttcggc ctgttcgtat cgcccagcac gacgacctcc ggcgtggcca
     5221 ccagtaccgg cacactagtt ccggacccca actacaatgg cggggcgtgt actatcgcac
     5281 tagtgaacac gacctgcaag ttcggcatgg atgatagcgc aaccagcggc gtcatcagta
     5341 cttacggaga tgtgattgcc agcagcgcag tggctgacgg cggcactggc ccgataagcc
     5401 agttgaactc caagttgctc ttcggggcga cggccggtct gaccgtccca gccggtatct
     5461 acaccggtag cgagtcactg atcgccaccg gtaccttcta gaccgactcc tgtaaacgct
     5521 cctaagcccc gcttctcctg gcggggcttt tgctgtgtcg gcaattgccg tgcgatacgg
     5581 ctaatgtaat atgtacaacc atttgttggc aagccaaacg ttagcgtgat aatatatcgt
     5641 tgtatagtct aaaacgatat aaaaaacagg agcaaatata aaaatgaaaa gtgcgttgga
     5701 gaatttggga cgcgttcgtg cgctttccct ggccattgct ttgcttgtgg ccgccagcgt
     5761 accgctgaca gc
//
LOCUS       DASSFD010000126         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_113810, whole genome shotgun sequence.
ACCESSION   DASSFD010000126 DASSFD010000000
VERSION     DASSFD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113810"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFH39_02915"
     CDS             <1..834
                     /locus_tag="VFH39_02915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6924752.1"
                     /translation="RLRDFEQFSREVFAQVIDPVPVIIACHNEQRSLPVTLTALARQE
                     QAVLPIVVDNDSTDRSAEIADRFGARVLFEPRRGKMLALQRGILSVLDNPALSDRPLL
                     FTDADTVMPAGWARHMAGQVSRRKDSMITGLRIFNRAEKPAQLAINGMRTAYVMGKNV
                     KRSMTRQTPVAVGQNMALSPLDRGPLGDAILKLPNYWPCEDAAIRDEVLDNGGTVRLS
                     YDRRSLTFTAGDRYGTVKAAGRRLLKGASCTVEQYDTLDTGAFVSRYDGKSRKRSNVG
                     W"
     gene            969..2153
                     /gene="tuf"
                     /locus_tag="VFH39_02920"
     CDS             969..2153
                     /gene="tuf"
                     /locus_tag="VFH39_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET6924753.1"
                     /translation="MADQFDRSKPHVNVGTMGHVDHGKTTLTAAITHVLAKRLPSKVN
                     VPRNYEDIDNAPEEKARGITIATSHQEYESEKRHYAHVDMPGHADYVKNMITGAAQID
                     GAILVVGANDGPLPQTREHVLLARQVGVPSILVFLNKMDLADAELVELVEEEIRDLLE
                     KNGFDRNAPIIRGSATKALEGDAASEDAIMELVKAMDDYIPEPKRELDKPFLMPIEDV
                     FSIKGRGTVATGRVEQGIVKINDEVEIVGIKATTKSVVTGVEMFKKNLDQGQAGDNVG
                     ILLRGIERDDIERGQVLAKPGTITPHTEFESEVYILTKEEGGRHTPFFKGYKPQFYFR
                     TTDVTGEVELPADKEMVMPGDTVTFNVKLLAPIAMEQGLRFAIREGGRTVGAGVVTKI
                     NK"
     gene            2246..2749
                     /locus_tag="VFH39_02925"
     CDS             2246..2749
                     /locus_tag="VFH39_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924754.1"
                     /translation="MNQLQLSAYNEARTIAREHGIADRLPMLYEVARTALGEHYPLPV
                     GTVCLLSDFAGDDISPFLGPESYIRPPIIKAVRELSASHIERNPLSHAAAYFVASAGF
                     RYLLSHERLGSVYDTPGMHQITDKLNSCAHRASLLKVGLPTPADVRHHLNRRAHGLRI
                     IRSDDDQ"
     gene            2770..3174
                     /locus_tag="VFH39_02930"
     CDS             2770..3174
                     /locus_tag="VFH39_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6924755.1"
                     /translation="MATSSGSEFVDYYELLGVEPSADLPDIRRAFIGKAKEHHPDAGG
                     SADVMRQLNVAYKTLMSETSKAAYDLLHSFHIGSTEPSDYKYHGGREVNDVDDMTDDE
                     IDSFLDDLWSEYRNGPSKPKPSIKQRFTSLFD"
     gene            complement(3177..3902)
                     /locus_tag="VFH39_02935"
     CDS             complement(3177..3902)
                     /locus_tag="VFH39_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924756.1"
                     /translation="MASDVEKFGLASSKWERLMAEQQFSVPSASGRLAIVLAYDMARD
                     DDPGILDPYPTFEKEARDLADRYSREGRKVELALQADRPAFRAIMHDRTISDITVLGN
                     GNLAAVHMPAPERPLDWFNLSKMTNHIKQGQIRQLFCGFTPRDLNVPFGLFAVAQPAD
                     VIAPVNHFLRPDHAQRDYRHLRPVSHADRLSYEDIKRSFPRKNKENLTQRAIVRQALA
                     RQWLKRHMTEGPAVHQLELSAEY"
BASE COUNT          913 a         1165 c         1151 g          890 t
ORIGIN      
        1 cgactacgtg atttcgaaca attcagtcgc gaggtattcg cgcaggttat cgatccggtg
       61 ccggtgatca tcgcctgcca taatgagcag cgcagccttc ccgtcaccct gaccgcgcta
      121 gctcgacagg agcaagcagt gttgcccatt gtggtcgaca acgacagtac cgaccggtca
      181 gctgaaatcg ctgaccgttt cggcgcacgg gtgctgttcg agccgcgccg aggcaaaatg
      241 ctggcgttgc aacgaggtat tctcagcgtc ttggataacc cggcgctcag cgatcggccg
      301 ctgcttttta ccgatgcgga tacggttatg ccggctggct gggcacgaca tatggccggg
      361 caggtgtcgc gtcgcaaaga cagcatgatc actgggctga ggatattcaa ccgcgccgaa
      421 aagccggcgc aactggcaat caatggcatg aggaccgcct atgtcatggg caagaacgtt
      481 aaacgatcga tgacgcgtca gactccggtc gcggtcggac agaacatggc gctgtcccca
      541 ctggacagag gtcctctggg ggatgccatc ctgaagctac ccaactattg gccatgcgaa
      601 gacgccgcca tacgcgacga ggtgctggat aacggcggca cggtacgtct gtcgtacgat
      661 cgccgttcgc ttacttttac ggctggtgat cgttacggaa ccgtcaaagc ggccggccgc
      721 cggttgctca agggtgccag ctgcactgtc gagcagtacg atacccttga tactggcgcg
      781 tttgtttcgc gttacgatgg caagtcgcgt aagcgctcaa atgttggctg gtagcccact
      841 tataggcgtc tttacaaact tcttagcttt ctctatacta gagcggtcac tttgtgtgag
      901 ttctagtatg ctcatgcaaa aagaacttaa taacggcaag taataacaat taaaggagca
      961 atccacctat ggcagatcaa tttgaccgca gcaagcccca cgtcaacgtc ggtactatgg
     1021 gccacgtcga ccacggtaag acaaccttaa ccgccgccat cacgcacgtt ttggctaagc
     1081 gccttccgag caaggtgaac gtcccgcgca actacgagga catcgacaac gctcccgaag
     1141 aaaaggcccg cggtattacc atcgccacct cccaccagga gtacgagtcc gagaagcgcc
     1201 actacgctca cgtcgacatg ccgggtcacg ccgactacgt caagaacatg attaccggtg
     1261 ctgcccagat cgacggcgcc atcctggttg tcggtgccaa cgacggtccg ctgccgcaga
     1321 cccgtgagca cgtgctgctg gcccgccagg tgggcgtgcc ttccattttg gtcttcctga
     1381 acaagatgga cctggccgac gccgagctgg ttgagctcgt cgaagaggaa atccgcgacc
     1441 tgctcgagaa gaacggtttc gaccgcaacg ctccgatcat ccgcggttct gctaccaagg
     1501 ctctcgaagg cgacgccgct tccgaggacg ccatcatgga actggtcaag gccatggatg
     1561 actacattcc ggagccgaag cgtgagctcg acaagccgtt cctgatgccg atcgaagacg
     1621 tcttctccat caagggtcgt ggtaccgtcg ctaccggccg tgtggaacag ggtatcgtca
     1681 agatcaacga cgaagtcgaa atcgtcggta tcaaggcgac caccaagagc gtcgtgaccg
     1741 gtgttgagat gttcaagaag aacctcgacc agggtcaggc tggcgacaac gtcggtatcc
     1801 tgctgcgcgg tattgagcgc gacgacatcg agcgtggtca ggtgctggcc aagccgggca
     1861 ccatcacccc gcacaccgag ttcgaatctg aggtctacat cctgaccaag gaagaaggtg
     1921 gccgtcacac cccgttcttc aagggttaca agccgcagtt ctacttccgt accaccgacg
     1981 ttaccggtga ggttgagctt ccggctgaca aggaaatggt catgcccggt gacaccgtca
     2041 ccttcaacgt caagctgctg gcgccgatcg ccatggagca gggtctgcgc ttcgccatcc
     2101 gcgaaggcgg ccgcaccgtt ggcgctggtg ttgtcaccaa gatcaacaag taattactgt
     2161 aagcgtaatg acttgccggg gagcgtctcg aaagagacgc tcttcttgat taagtagtta
     2221 tttaaccttg ttgtcacgca attccatgaa tcaactacag ctatcggcct ataacgaagc
     2281 tcgcaccata gcccgggaac acggtattgc cgacaggctc ccgatgcttt acgaggtggc
     2341 tcgaaccgca ctcggtgagc attacccgct gccagtcggt accgtctgcc tgctatccga
     2401 cttcgccggc gacgatatct caccgtttct aggccctgag tcgtatattc ggccgcccat
     2461 tattaaggcc gtgcgggaac tatcagcgag tcacattgag cgtaacccac taagccatgc
     2521 tgctgcctat ttcgtagcca gcgccggctt tcgttaccta ttgtcacatg aacgactggg
     2581 ttcggtttac gacacgcccg gcatgcatca gattacggat aaattgaaca gctgtgcgca
     2641 ccgggcatca ctactgaagg taggcctacc gacaccggcc gacgtccgcc atcatttgaa
     2701 ccgtcgtgct catggcttac gcattattcg tagtgacgat gaccagtagg atgcaggtat
     2761 aatagagcag tggcaacctc tagcggttcg gaattcgtgg actactatga gctgctcggt
     2821 gtggagccga gcgctgatct gccggacatt cgccgggctt ttattggcaa ggccaaggag
     2881 caccatccgg atgccggggg ctcagccgat gtcatgcggc agctgaacgt cgcctataag
     2941 acgctgatga gtgagacttc caaggccgct tacgacttat tgcatagctt tcacattggc
     3001 agcacggagc cgagcgatta taaatatcac ggcggccgag aggtgaatga cgtcgatgat
     3061 atgaccgatg atgagattga tagctttttg gatgacctct ggtcggagta tcgtaacggt
     3121 ccttctaagc cgaagcccag cataaagcag cggtttacgt cgctgtttga ttagcgctag
     3181 tattcagcag acagttcaag ctggtgtacg gccgggccct cggtcatatg ccgtttcaac
     3241 cactgtcgtg ccagtgcttg ccgcacgata gcccgttgtg tcagattctc cttgttcttt
     3301 cgaggaaagc ttcttttgat gtcttcgtag cttaatcgat cggcatggct gaccggccgg
     3361 agatgccggt agtcacgctg ggcgtgatca gggcggagaa aatggttcac cggtgcgata
     3421 acgtcagccg gctgggccac ggcaaacagc ccgaacggta cgttcagatc tcgcggggtg
     3481 aacccgcaga aaagctgtct gatctgccct tgtttgatgt ggttggtcat ttttgacaga
     3541 ttgaaccaat cgagcggccg ctccggcgcc ggcatgtgca cggccgccag gttaccgttg
     3601 ccgagcaccg tgatatcaga gatggtgcga tcgtgcatga tcgcccggaa ggccggccga
     3661 tcggcttgca gtgccagctc gacctttcta ccctcgcggc tgtaacggtc tgccaagtcg
     3721 cgagcttctt tttcgaaggt agggtagggg tccagaatgc ctgggtcatc gtcgcgggcc
     3781 atgtcatacg ccagaacgat agccagccgg ccgctggccg acggcacgct gaactgctgc
     3841 tcggccatga gccgctccca cttcgaactg gccagtccga atttctctac atcgcttgcc
     3901 ataatccgta taacagtata atattgagtt cgaaatatca attgcgatga gaactcgtcc
     3961 ggtatcactc gagatataaa ttttcgtact caggataagc caatacattg ccttatcctt
     4021 ccgtgcggca cttatcactc atcaccccgg aagaggttac atcgcctaaa gtacaggagt
     4081 acttcattat ggctgacgcc aaagcagacc agaaacagc
//
LOCUS       DASSFD010000127         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_528371, whole genome shotgun sequence.
ACCESSION   DASSFD010000127 DASSFD010000000
VERSION     DASSFD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528371"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..238
                     /locus_tag="VFH39_02940"
     CDS             47..238
                     /locus_tag="VFH39_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924757.1"
                     /translation="MTDNTPQYDALTRDAAHVQKSWVFHCTAPGMYRATLFVDQRGEI
                     GLRFTDFTESCHGRAEVRD"
     gene            344..1108
                     /locus_tag="VFH39_02945"
     CDS             344..1108
                     /locus_tag="VFH39_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET6924758.1"
                     /translation="MFPVARTAYRRMVGDTMFLAAELATPPALKGAVCYNRYGGYFVP
                     ESARYRIDAGTVLKGDVWEPDTLEFIRSRCADGGVVHAGTFFGDMLPGICSALAPGAR
                     LWAFEPNPESYRCAKVTAAINGLDNIELTRAGLGEAPGRLFLRTRKDDGSSLGGTCHI
                     EERPSGEGETIDIVRIDDVVNGPASVIHLDVEGHERAALAGALQTLQRCRPILLLEEF
                     TDALTGSEWFAENVLSLGYRRTGRVHDNAVFEATTT"
     gene            complement(1095..>3668)
                     /locus_tag="VFH39_02950"
     CDS             complement(1095..>3668)
                     /locus_tag="VFH39_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924759.1"
                     /translation="VSTLVREAAVLMEELEQLLAIPCKGLPVGVLVEGSVQLPQSRAA
                     APVVLTLVSQPGQMAARDLPRRRGMEARAPFQTAVRILPAPVAEVEVLRPPALPGLAV
                     LAQSDRAEAEVVVASQEEASVVPAELEAMEKSSLSRRISQMDRRNLIKSVGAALGAFA
                     LPFPIGARMGRGSIASGAQNNLALGVNLQQPNYYSSEQPFLNILYNNWIDAGSVSRGT
                     TSWLTSSFSKYSGTVDSNGWPTQIQDGTANYMLALNALNASNAGAGPTYRSGQYYVYY
                     NGTGAVSYGNDATIVSHNSGPAPYDWADVINVSTPSSNGILIKVTSTGAAPNNIRNMA
                     LVHNSETSSFLAGNLWSSAFLSRLSNFAMVRTMDWRNTNNATWIVNWSDEPGTTIGGY
                     GGMVPWPIIVNLANTANVHLWVNVPVGASSAYCNSLMSYLAGALNPNINLIVEFSNEV
                     WNGTAQQSVALANGKALWANWATYGTVATYLSYEGYMLQQICNAAVSAFGSANVGAGK
                     RVQVVAGAQYQNGGVLDDVMNCPVYTANGGQALYGNFPIAGCTFAWYFNIGSKHIPAS
                     WLGYSSSTFVANAYSQFTSGGLIPQDTAGGYAYYKSTVDYVVNQMATKYGGLPCFGYE
                     GGEGETPNQDFDQLYNNASAGTIARSTTYSTSTSPYRVDGSNNLYLCTTGGTTGSSAP
                     TFNTALGGTTADGTVVWTRVQSATVSWVANWTPTVGDVVKDSNGNFQVALTVAAPTGA
                     SAPTWGTTPKSITSPGSQTTDGGVTWTCIRDDSWTALEIAYSRSSQINSAYTSAMNYW
                     KGKSAGCHYFCQYMDCNAPSKYGLWGASESFEDTVSPLSSAPQKWQAIVAWATANPKW
                     W"
BASE COUNT          746 a         1074 c         1077 g          771 t
ORIGIN      
        1 ccgagttcaa gaggctttcc ggataactca tccgaagaat cccgcaatga ccgataacac
       61 cccccaatac gatgccctca cccgcgatgc cgcccacgtg caaaagtcgt gggtatttca
      121 ctgcaccgca cccggtatgt atcgggcgac gctatttgtg gatcagcggg gagagatcgg
      181 cttgcggttt acggacttca cggaaagttg ccacggacgg gctgaagtga gagattgata
      241 gagcgccggt cggcgcatgg gtggaataag taataacggg cgagaaacgc tcggaagggg
      301 tcatgtcatg aagtctgcgg tgaaaaaact catcccgagg cagatgtttc ctgtcgcaag
      361 gacggcatac cggcgcatgg tgggagacac catgtttctg gcggctgagc tggcaacgcc
      421 gcctgcgctc aaaggcgccg tctgctacaa ccgatatggc ggatatttcg tgccggagtc
      481 ggcgcgctac cggatcgatg ccgggacggt gctgaagggt gacgtgtggg agcctgacac
      541 tttagagttc atccggagcc gttgtgcgga tggtggcgtc gtccatgccg ggacgttttt
      601 cggggatatg ctgcccggca tatgcagcgc tcttgctcct ggtgcccggc tgtgggcgtt
      661 cgagcccaac ccggaaagct accgatgcgc aaaagtgact gccgcgatca acgggctgga
      721 taatatcgaa ctcacgcgcg cagggctcgg agaagctccg gggcggctat tcctgcgcac
      781 cagaaaggat gatggctcct ccctcggagg aacctgccac attgaggagc gcccgtcagg
      841 cgagggggaa acgatagaca tcgtgcggat tgatgatgtc gtgaatggcc cggcgagcgt
      901 aatccacctc gatgtcgagg gccacgaacg ggcggcgctt gccggggctc tccagaccct
      961 tcagaggtgt aggcccatcc tcttgctgga ggagttcacg gacgctctca ctggtagcga
     1021 atggtttgcc gagaacgtct tgagccttgg atatcgaagg accggacgcg tacacgacaa
     1081 cgcggtgttc gaggctacca ccacttagga ttggccgtcg cccaagcgac gatagcctgc
     1141 cacttctgag gagctgaact cagcggggaa acggtatcct caaagctctc agaggcgccc
     1201 caaagcccgt atttactcgg tgcgttgcaa tccatgtatt gacaaaaata gtggcatccg
     1261 gcgctcttgc ccttccagta attcatggcg gaagtgtagg cgctattgat ctgcgagctg
     1321 cgggagtacg cgatctcaag cgccgtccag gaatcatccc gaatacacgt ccacgtcacc
     1381 ccgccatcgg tcgtctgcga cccgggcgac gttatagact tcggtgtcgt tccccacgtg
     1441 ggggcgcttg cgccagtggg ggccgccacg gtaagggcca cttggaaatt tccgttgcta
     1501 tctttgacga cgtccccgac cgttggggtc cagttcgcca cccatgagac agttgcgctc
     1561 tgcacccgcg tccatacaac tgtgccatcg gcggtcgtgc ctccgagagc tgtgttgaag
     1621 gtaggagcgc ttgaacccgt cgtgccgccc gtagtgcaaa gatagagatt attgctgccg
     1681 tccacccgat agggagacgt gctcgttgag taagtcgtag aacgcgcaat ggtgccggcg
     1741 ctcgcgttat tgtagagctg atcgaaatcc tgattgggcg tctctccttc gccgccctca
     1801 tatccgaagc acgggaggcc gccgtactta gtcgccatct ggtttacgac gtaatcgacc
     1861 gtcgatttgt aataggcata tccgccggcc gtgtcctgag ggatgagacc tccagaggtg
     1921 aactgagaat aggcgttcgc gacgaacgtg gatgatgagt atccgagcca actcgccggg
     1981 atgtgttttg atcctatgtt gaagtaccag gcaaaagtac acccagcgat cgggaaattc
     2041 ccataaagcg cctggccgcc atttgcggtg tacacgggac agttcatcac gtcatctagg
     2101 acgccgccgt tctggtactg cgcgcctgcg actacttgaa ctctcttccc tgctccgacg
     2161 ttcgccgatc cgaaagcgct tacagcggcg ttacagattt gctgtagcat gtacccttcg
     2221 tagcttaagt acgtcgctac cgttccatag gtcgcccagt ttgcccaaag agcctttccg
     2281 ttagccaacg cgactgattg ctgcgcagtt ccgttccata cctcgttcga aaactcaacg
     2341 atgaggttga tgtttggatt gagggctcca gccagatagc tcatcaggga attgcagtac
     2401 gcgcttgaag ctccaactgg cacgtttacc cataggtgca cattcgcagt attggcaaga
     2461 ttgacgatga tcggccatgg aaccattccg ccgtaaccgc ctattgtggt ccctggctca
     2521 tctgaccaat tgacgatcca agtggcattg ttcgtatttc gccagtccat ggtccgtacc
     2581 atggcgaaat tggagagccg cgacaggaac gccgatgacc agagattccc ggcgagaaag
     2641 ctactcgtct ccgaattgtg aacgagcgcc atattgcgga tgttgttcgg agccgcaccg
     2701 gtcgatgtga ccttgatcag aatgccgtta gatgaagggg tcgagacatt gatgacatca
     2761 gcccagtcat acggggcggg gcccgagttg tggcttacga tcgtcgcgtc gttcccatag
     2821 ctgacggcgc ctgttccgtt gtaatagacg tagtactgtc cgctcctgta tgtcgggcct
     2881 gctccggcgt ttgaggcgtt cagcgcattg agcgcaagca tgtaatttgc ggtgccgtcc
     2941 tggatctggg tcggccagcc attggaatcg acggtcccgg aatacttgct gaagctgctc
     3001 gtgagccagc tcgtggtgcc gcgcgatacg gaccccgcat ctatccaatt gttgtaaagg
     3061 atattgagga atggctgttc tgaggagtag tagttcggct gctgaaggtt cacccccagg
     3121 gcgagattgt tctgggcacc agaagcgatg ctaccgcggc ccatccgcgc accgattggg
     3181 aacggcaatg cgaacgcccc aagcgcggcg cccaccgact tgatgagatt gcggcggtcc
     3241 atctggctaa tacgtcgtga taacgacgac ttttccattg cctccagctc cgccggcacc
     3301 acagacgcct cctcctgaga ggcaactacc acctccgcct ccgcccgatc cgattgcgcc
     3361 agcaccgcca gtcccggcag tgccggaggt cgtagaacct ccacctccgc caccggtgcc
     3421 ggcaaaatac gcaccgccgt ttggaacgga gcccgcgcct ccattccccg ccgtcgtggt
     3481 agatcccgag ccgccatttg ccctggttgt gaaaccagtg ttaagaccac cggtgccgcc
     3541 gctctggact gcggcagctg cactgacccc tccaccagca cccccaccgg taggcccttg
     3601 cacggaatcg ccagcagttg ctccagctcc tccattagga ccgccgcctc ccgaaccaac
     3661 gtgctcac
//
LOCUS       DASSFD010000128         7403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1800566, whole genome shotgun sequence.
ACCESSION   DASSFD010000128 DASSFD010000000
VERSION     DASSFD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800566"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..888
                     /locus_tag="VFH39_02955"
     CDS             31..888
                     /locus_tag="VFH39_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6924760.1"
                     /translation="MTKTLSLQQLEQKANDIREDIIRMLEHAGSGHSAGPLGLADIFT
                     ALYFDVLKHDPKKPDWDERDILILSNGHCVPVRYATMAEAGYFDKKELLTLRKFGSRL
                     QGHPERVRLPGLESTSGPLGSGLSQACGIALALRMNQQPHRFVYVVMGDGEQDEGNVW
                     EAAMLAGKYRLDNLIAITDRNNIQIDGFTENVMPLEDFKGKWESFGWHVIEIDGNDIE
                     AVIDACAMGRAIHEKQVMIVAHTIPGKGVDFMENDFHWHGMPPNHEQAIKALHELRTL
                     GGKIRSEHE"
     gene            1010..1216
                     /locus_tag="VFH39_02960"
     CDS             1010..1216
                     /locus_tag="VFH39_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924761.1"
                     /translation="MRVRGFPFGYSATTGDDTATCMNAADPIYAYHPVTASFQTASVW
                     FDLIVYGVVLTAAYLLIDQTKVRT"
     gene            1213..2187
                     /locus_tag="VFH39_02965"
     CDS             1213..2187
                     /locus_tag="VFH39_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET6924762.1"
                     /translation="MSDMLLVDITSKDLASEPIRKGFGRGLKAAGELDVNVVAACADL
                     TDSTQMSIFKEAFPDRFVEIGVAEQNLVTVGSGMAAMGKIPFVSSYAAFMPGRCWEQI
                     RTTICLNDQPVKLIGSHAGVSVGPDGATHQMLEDLVLMRALPNMVVLAPCDSLEAEKM
                     TLAMAKDKRPNYMRLAREATPIITTDKTPFEIGKAYVYSPGADVTIIATGTMTYQALV
                     AAEQLYKDGIEAEVVHVPTIKPLDTATILKSVRKTGCVVTAEEGQIIGGLGGAIAELL
                     AEEYPAPLKRIGMQDRFGESGEPTELLEHFGLDAKHIRLAAHHVVDRK"
     gene            2184..3074
                     /locus_tag="VFH39_02970"
     CDS             2184..3074
                     /locus_tag="VFH39_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET6924763.1"
                     /translation="MNWQTLAILQTIPAAAQVLMWRHIARGKRKFKAATINALTLLEL
                     WVCATVLAVCLGGIHLAQIRPYVGLMLLSGLAYAASTIFMYKSLEHLAAGIFSILQTS
                     VTLFTIVLSFTFLHTTFRGSQWVGAAVLMLTIWTTLWLASKENKHLEKAAKWQTGVGY
                     CLASALTLAIGLTMEKYMLSHVGTATYLVFGNGLQLVGAVVIAVAIDRGQGLTQLKKN
                     KNVMSWLLGSGIMRGLAGVLFILAQVGSNNVALVSVIANFKIIIIAIFGALILREHKY
                     FFHKLTTASLAVVGLALTLR"
     gene            3164..4024
                     /locus_tag="VFH39_02975"
     CDS             3164..4024
                     /locus_tag="VFH39_02975"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6924764.1"
                     /translation="MGRARESHFAFGAFNLDDQATLLAVCQAAAKVQAPVLVEVSQGE
                     VDTLGLDNVRDLVDNYKREFGLEIYVNLDHSPSVENAKKGIEAGFEFIHIDISQANHD
                     ATDEEIIAATREVVEYAKFTGALVESEPHYFGGSSNLHNETINYEEIKKTFSTPDGAR
                     HFVEATGIDTFAAAVGNLHGKYPVPKVLDLELLKQIREAIGCNISLHGGSDTPGHYFK
                     SAVEIGVTKININSDMRYAYRTSLEQALKDNPDEYSVAKLIAKHVVPEVQKVVESKLA
                     DFNSHGKAVA"
     gene            4090..5091
                     /locus_tag="VFH39_02980"
     CDS             4090..5091
                     /locus_tag="VFH39_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6924765.1"
                     /translation="MSVQPDVISIGDIVTDVFIKLKDDYEHTYSNEHGKWLAMPFGTK
                     IPFDNAETIKAVGNASNAAVAFARLGLNSAFVTNVGEDHEGRDMISTLHKQKVDSRFV
                     RVNPDKKSNVHYVIRFGAERTILIKHEEYDYHWPHLRPSETPRWVYFSSISEHALPYH
                     DQVADWLDANPDVLLAFQPGTFQMEAGVERLQRIYKRSSVLILNREEAALVGGGNHED
                     IHDLFNKLHALGPHVVVITDGPAGAYASDGQNRWSMPLYPDPAEPIDRTGAGDAFAST
                     FVAALAKGNTIEGALQWAPINSMSVVQKVGAQAGLLSEHELLHWLSQAPEWYKPSRM"
     gene            5138..6367
                     /locus_tag="VFH39_02985"
     CDS             5138..6367
                     /locus_tag="VFH39_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924766.1"
                     /translation="MTRILSEMVGASERLFRSELAKLESLAGHPNADIRLTADIVQAT
                     KVKLKELGLDQHDTHGPELYAALQERLKQDDARLLASLRSSAGDPDDVLSGVTHALQS
                     LPLAGSCLALKSAQAKRLLRKTPPKKAMKQLGYRSLDSMLKHEPAAAIFAVAWNVESA
                     AWRKALLESYGRLQASDFETRAITIIHPKSAKWLRVGAELVKRQKHSVMCFPELGAVV
                     LLPLPVADQPPAFTTITLIMALTSMNDIRTLSSYLRLNQFRPDFGAIVRDVARHDPYL
                     TSAMLDQPVAWRVIQRFYARFKHVFDPEAFELALQSHDLSWHSIERSLERLEPSLGFW
                     RGTEHLGLLHDRQPVSLNIIDVALGYCNGLPFEQRIVHHLRQALHQEILLQYLHKDNV
                     EQLVRLQLQPELVSELA"
     gene            6399..6968
                     /locus_tag="VFH39_02990"
     CDS             6399..6968
                     /locus_tag="VFH39_02990"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924767.1"
                     /translation="MYQICVSGAAKGDSVEEGRELATKLGAAIARAGHSLLTGATIGL
                     PNYAAEGYKAAGGTMSVGISPASSKIEHVMKYRLPVEAYDTILFSGLHYVGRDTLLIT
                     SSDAVVSIGGRLGTLHEFTIAMETDTPIGFLQGAGGVSEEIQTLMSLAHPLRQGAFVA
                     FEDDSDRLIHELTKFLDGERKKYHNLYQE"
     gene            6990..>7403
                     /locus_tag="VFH39_02995"
     CDS             6990..>7403
                     /locus_tag="VFH39_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924768.1"
                     /translation="MAATKTTKKKQAATTKSKGGNWSAAYAKPLLIALGVVIIVGTVW
                     FVLVARHNANAALLNSQKSSQGTKSPMHSNNEAIKAAAALYRAYLPLKAQTVADVGNN
                     PNVEPANVQVVRASEVYFTKSLYNDILKDYMTQYQQ"
BASE COUNT         1717 a         2126 c         2165 g         1395 t
ORIGIN      
        1 taagcagaaa cgctaatgca agaccaaaac atgacaaaaa cactgagcct gcagcaactg
       61 gaacagaaag cgaatgacat ccgcgaggac atcatccgca tgctggagca cgccggcagc
      121 ggccacagcg ccgggccgct cgggctggcc gacatcttca cggcactcta cttcgacgtg
      181 ctcaagcacg acccgaagaa gcctgattgg gatgagcggg acatcttaat cctgagcaac
      241 ggacactgcg taccggtccg gtacgccacg atggccgaag ccggttattt tgacaaaaaa
      301 gagctgctga cgctgcgaaa gttcggcagt cggctgcaag ggcacccgga acgagtgcgc
      361 ctgccggggc tggagagtac cagcgggccg cttggctcgg gcttgagcca ggcctgcggt
      421 atagcactgg ccttacgcat gaaccagcag ccgcaccgct tcgtgtacgt ggtgatgggc
      481 gacggcgagc aggatgaggg taacgtgtgg gaagccgcca tgctggccgg caagtaccgc
      541 ctggataacc tcatcgccat caccgaccgc aacaacatcc agatcgacgg ttttaccgag
      601 aacgtcatgc cgcttgaaga tttcaagggc aagtgggaga gcttcgggtg gcacgtcatc
      661 gaaatcgacg gcaatgacat tgaggcagtg attgacgcct gcgccatggg ccgcgccatc
      721 cacgaaaagc aggtgatgat cgtggcgcac accatacccg gcaaaggcgt ggacttcatg
      781 gagaacgact tccactggca tggcatgcca cccaatcacg aacaagccat aaaagccctg
      841 cacgaacttc gcaccctcgg aggcaaaatc cggagcgaac atgagtaaat ggattgcgtt
      901 caccatggga atagttatcc tgataatcat cttcggagcc gcgcttattc ctattaggcg
      961 aggcgccaag atccattgcg tcggaagtac agctacgtac gatacgcgca tgcgggtacg
     1021 aggttttcca ttcggctaca gcgcgacgac cggcgacgac acggcaacat gtatgaacgc
     1081 ggctgacccc atttacgcct atcaccctgt gaccgcatcg tttcagacag catcggtatg
     1141 gttcgacctg attgtatacg gagtagtatt gacggctgcc tatttattaa tcgatcagac
     1201 gaaggtccga acatgagtga catgcttttg gttgatataa cttcaaaaga tttggcgagc
     1261 gaacccatcc gtaaaggttt cgggcgtggg ctgaaggcgg ccggtgaact ggacgtgaat
     1321 gtggtagcgg cttgcgccga ccttaccgac agtacgcaga tgagcatatt caaagaagcg
     1381 tttcctgacc ggttcgtcga aatcggtgtg gccgagcaga acctggtgac ggtgggctcc
     1441 ggcatggcgg caatgggcaa gattcccttt gtgagcagtt atgcggcctt catgcccggc
     1501 cgctgctggg aacagatccg taccactatc tgcctgaacg accagccggt caaattgatt
     1561 ggttcgcacg ccggcgtgtc ggtcggtccg gacggtgcca cgcaccagat gctcgaggac
     1621 ctggtgctga tgcgggcgtt accgaacatg gtggtactgg caccgtgcga cagcttggaa
     1681 gccgagaaga tgacgctggc catggcgaag gacaaacgtc cgaattacat gcggttggcg
     1741 cgcgaagcaa cgcccatcat cacgaccgac aaaacgccgt tcgaaatcgg caaagcgtat
     1801 gtctacagcc ctggcgccga tgtaactatc atcgctaccg gcaccatgac gtaccaggca
     1861 ttggtggctg ctgagcagct atataaagat ggtatcgagg cggaagtggt gcacgtgccg
     1921 accatcaagc cgctcgacac cgcgacgata ctaaaaagcg tcaggaagac cggctgtgtg
     1981 gtaacggccg aagagggcca gataatcggc ggcctaggcg gtgccattgc cgagctgctg
     2041 gccgaggagt atccggcacc gctcaagcgc atcggcatgc aagaccggtt cggcgaatca
     2101 ggcgaaccga cagagttgct ggagcatttc gggctcgatg ccaagcacat ccgcttggct
     2161 gcgcaccacg tcgtcgatcg caaatgaact ggcagacact cgcgatattg cagaccattc
     2221 cggcggccgc ccaggtattg atgtggcgac acatcgcccg cggcaagcgg aagttcaaag
     2281 ccgccaccat aaatgcgctg acattattgg agctgtgggt gtgcgccacc gtgctggccg
     2341 tatgcctggg tggtatccac ctggcacaga tccgacccta cgtagggctt atgttgctgt
     2401 ctgggcttgc ctatgcggcc agtaccattt tcatgtacaa gtcgctcgaa catctggcgg
     2461 ccggcatatt cagtattctg cagacctcgg tgacgctctt caccatagtt ttgtccttca
     2521 ctttcttgca caccaccttt cgcggcagcc aatgggtggg cgctgccgtg ctgatgctga
     2581 ccatatggac caccttgtgg ctggctagca aagaaaacaa gcatctcgag aaagcggcca
     2641 agtggcagac gggcgtcggc tattgtctgg cgtcagcgct caccctggcc atcggcctga
     2701 ccatggaaaa gtacatgctc agccatgtcg gcaccgccac ctatctggtg tttggcaacg
     2761 gcctgcagct agtcggcgcc gtagtcatag ctgtggccat cgatcgtggg caaggcctga
     2821 ctcagcttaa gaaaaacaaa aacgtcatgt cgtggctgct cggttcgggt atcatgcgcg
     2881 ggctggcagg cgtgctgttc attttggcgc aggttggcag caacaacgtg gcgctggttt
     2941 cggtgatcgc taacttcaag ataatcataa tcgccatctt cggtgcactt atcctgaggg
     3001 agcataaata cttcttccat aaattgacga ccgcatcatt ggcggtggtg ggactggcat
     3061 tgacgctgcg ctgattgcca gctaagcata aacactttac aatcgaagta tgcctttaac
     3121 tcttacacaa atccgcgaca actgtaaaaa agcccgtgac agcatggggc gcgctaggga
     3181 gagccacttc gccttcgggg ccttcaacct ggacgaccag gcaacgctgc tggcggtctg
     3241 ccaggcggcc gccaaagtcc aagcgcccgt cttggtagag gtgagccaag gagaagtcga
     3301 cacgctcggt ctcgacaacg tccgcgacct ggtcgataac tacaagcgag agttcggcct
     3361 cgagatatat gtgaacctcg atcacagccc aagcgtcgag aacgccaaga aaggcatcga
     3421 ggccggcttc gagttcatcc atatcgatat atcccaggcc aaccacgacg ccaccgacga
     3481 agagatcatc gccgctaccc gcgaggtggt ggagtacgcc aagttcacgg gcgcgctggt
     3541 ggagagcgag ccgcactact tcggcggtag ctccaacctg cacaacgaga ccatcaacta
     3601 cgaggagatt aagaaaacct tcagtacgcc cgacggtgcc cggcacttcg tggaagccac
     3661 cggcatcgac actttcgccg ccgctgtcgg caacctgcat ggcaaatacc cggtgccgaa
     3721 agtgttggat ctggagctgc tgaagcagat tcgcgaagcc atcggctgca acatatcttt
     3781 acatggcggc agcgacaccc ccgggcatta cttcaagagc gcggtcgaga tcggcgtcac
     3841 gaagatcaac attaatagcg acatgcgcta tgcctaccgg accagcctgg agcaggcact
     3901 taaggataac ccggacgaat actcggtcgc caagcttata gccaagcacg ttgttccaga
     3961 ggtgcagaag gtggtcgaga gcaagctggc ggacttcaac tcgcacggca aggccgtggc
     4021 ctgaacttgc gccctagcgg agcgtatggc acaatcgaag cagacaaagc ataaacagaa
     4081 agtggtggga tgagcgtaca acctgacgtc atcagcatcg gcgatatcgt gaccgatgtg
     4141 ttcataaagc tcaaagacga ctacgagcac acgtatagca acgaacatgg caagtggctg
     4201 gccatgccgt tcggcaccaa gataccgttc gacaacgccg agaccatcaa ggcggtgggc
     4261 aatgcgtcca acgccgccgt ggcgttcgcc cgtctggggc tgaacagcgc attcgtgacc
     4321 aacgtcggcg aggaccacga gggccgggat atgatcagca cgctgcataa acaaaaggtg
     4381 gacagccgct tcgtacgcgt caatccggac aaaaagagca acgtccacta cgtcatccgc
     4441 ttcggggccg agcgcaccat acttatcaag cacgaagagt acgactacca ctggccgcac
     4501 ctgcgcccga gcgaaacgcc tcgctgggtg tacttcagct ccatttcgga gcatgcgttg
     4561 ccttatcatg accaggtagc cgactggctc gacgctaacc cggacgtctt gttggccttc
     4621 cagcccggta cgttccagat ggaagccggc gtcgagcggc tgcagcggat ttataagcgt
     4681 agctcggtgc tcattttgaa ccgggaggaa gcggcattgg tcggcggcgg caatcacgag
     4741 gatatccatg acctgttcaa caagctgcac gcactggggc cacatgttgt ggtcattact
     4801 gatggtccgg ccggtgccta tgcttcagat ggccagaacc gctggagcat gcccctgtat
     4861 ccggacccgg ccgaaccgat cgaccgtacc ggcgcgggcg atgcgttcgc cagcacgttc
     4921 gtggcggcgt tggccaaggg caacaccatc gaaggcgcgt tgcagtgggc tcccatcaac
     4981 tccatgagcg tggtgcagaa ggtcggcgcc caggcaggtc tgctttcgga gcacgagtta
     5041 ctgcactggc tcagccaggc cccggagtgg tacaagccaa gccgcatgta gcgtaagctt
     5101 tatatgcccc tccgcttatg gtaatctggt caataagatg acacgtatcc tgtcggaaat
     5161 ggtcggcgcc agcgaacggc tgttcaggtc cgaactggcc aagctggaga gtctggccgg
     5221 ccatccgaac gccgacatcc gactgaccgc cgatatcgtg caggctacca aagtcaagct
     5281 gaaggaactc ggcctcgacc agcacgacac gcacggtcct gagctgtatg cggcgcttca
     5341 ggagcggttg aagcaggacg acgcccggct actggcttcg ttgcgttcat cggccggcga
     5401 ccctgacgac gtattgagcg gtgtgacgca tgccttgcag tctttaccgc tggctggtag
     5461 ctgcctggcg ctcaaaagcg cccaagccaa gcgtttactt cgtaagacgc cgcccaagaa
     5521 agccatgaag cagctcggct accggtcgct tgattcgatg ctgaagcatg aaccggctgc
     5581 ggcgattttc gcagtggcct ggaatgtcga atcggccgcc tggcgcaaag cgctactcga
     5641 gtcgtacggc cggttgcagg ccagcgattt cgaaacccgg gcaatcacca ttattcatcc
     5701 caaatcagcc aagtggttac gcgtcggcgc tgaactggtc aagcgccaga agcacagcgt
     5761 gatgtgcttc ccggaactag gcgccgtcgt actgttgccg ttgccggtag ccgaccagcc
     5821 gccggctttt acgaccatca cgctcatcat ggcgctgacc agcatgaatg acatccgcac
     5881 gctcagcagc tacctgcggc ttaaccagtt ccgaccggac ttcggtgcca tcgtccgcga
     5941 cgtggcgcgc catgacccgt accttacatc cgccatgctc gaccagccgg tagcctggcg
     6001 ggtcatccag cggttttacg cccgtttcaa gcacgtcttc gaccccgagg ctttcgagct
     6061 ggcgctgcag tcgcacgatc tcagctggca ttccatagag cgctcactgg agcggcttga
     6121 accgtcgttg ggcttctggc gaggtactga gcatctggga ttgctgcacg atcggcagcc
     6181 ggtatcgttg aacataatcg acgtggcgct agggtactgc aacggtttgc cgttcgagca
     6241 acggatcgtg catcatctgc gtcaggcctt gcaccaggaa atcctgttac aatatttgca
     6301 taaggataat gtcgagcagt tggtacggtt gcagctgcaa ccggaactag taagcgagct
     6361 cgcataagga aaatcataaa aaagggtgga ctaaaaccat gtatcagatc tgtgtgtcgg
     6421 gagcggccaa aggtgacagc gtagaagagg gcagggagtt ggcgaccaaa ctgggggcag
     6481 ccattgcccg ggcgggccac agcctgctga cgggagcgac catcgggctg ccgaactacg
     6541 ccgccgaggg ctacaaagca gcgggcggca ccatgagcgt cggcatcagc ccggccagca
     6601 gcaagatcga gcatgtcatg aagtatcgcc tgccggtgga agcgtacgac accatactgt
     6661 tcagcggtct gcactacgtc ggtcgcgata cactgctcat caccagcagc gatgcggtcg
     6721 tcagcatcgg cggccggctc ggcacgctgc acgagttcac catcgccatg gagaccgata
     6781 cacccatcgg cttcctgcag ggcgccggcg gcgtatccga agaaatccag acgcttatga
     6841 gcttggccca cccgctgcgc cagggagcct tcgtggcctt cgaggacgac tccgaccggc
     6901 tcatccatga gctcacgaaa ttccttgacg gcgaacgtaa aaagtaccac aatctctacc
     6961 aagaataagc gtaacggtga ggggacggta tggctgcgac taaaacgacc aagaagaagc
     7021 aggctgcaac taccaaaagt aaggggggca actggtctgc ggcctacgcg aagccgctgt
     7081 tgattgccct gggggtggtc ataatcgtcg gaaccgtctg gttcgtactg gttgcgcgcc
     7141 ataatgccaa cgccgctctt ctgaatagcc agaaatcatc ccaaggcacc aaaagtccga
     7201 tgcacagcaa taacgaggcg atcaaagctg ccgccgcact ctaccgagct tatctccccc
     7261 tcaaagccca aacagtggcc gacgtcggga ataatccgaa tgtcgagccg gccaacgtcc
     7321 aggtcgtgcg ggccagcgaa gtgtacttca ctaaaagctt atacaatgac attctcaaag
     7381 attacatgac gcagtatcag cag
//
LOCUS       DASSFD010000129         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1167949, whole genome shotgun sequence.
ACCESSION   DASSFD010000129 DASSFD010000000
VERSION     DASSFD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167949"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFH39_03000"
     CDS             <1..348
                     /locus_tag="VFH39_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET6924769.1"
                     /translation="RGRRDIVFAMLGITFLLGLGFLGMELHEFHHMVLDGNSWRKSAF
                     LSSFFTLVGTHGAHITSGLIWLLAMVPKLARQGLTPVNVRRLTLFSLFWHFLDVIWIF
                     IFTIVYMFGVIKP"
     gene            345..722
                     /locus_tag="VFH39_03005"
     CDS             345..722
                     /locus_tag="VFH39_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET6924770.1"
                     /translation="MNKPEKIVVGSHEEPPVNLATYIIGYVLSLGFTLTAYELVVHRV
                     LSFAWLLTDIIVLAFGQFIVQLVCFLHLGLERKPRWKLLVFGMMISIILIIIIGTIWI
                     MANLNYNMLPRNLNQYMHTNEGL"
     gene            722..1579
                     /gene="cyoE"
                     /locus_tag="VFH39_03010"
     CDS             722..1579
                     /gene="cyoE"
                     /locus_tag="VFH39_03010"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET6924771.1"
                     /translation="MNLRGYYSVLKPQRTLFNVVTTLAGYLLATRWHIHWSVLLGVLG
                     GTTLVVASACAANNCTDRNLDARMPRTRKRVLVTKQVSVQRAAWLGLVLGIAGFVLLG
                     LYVNWLTVLLGAIGYIDYVVLYAWSKRRTPHSTLIGTVSGAVPIMAGYTAATGRVDLT
                     ALLLGLILTAWQMSHFYAIGIFRHADYAAGKLPIWPVVKGDRSARDWIITYTLLFVLC
                     SAALWAVGAAGVVYLVCMTAIGVAWLYVAVAGLKRPDLMRWARQMFGYSLLVILASSL
                     LIAVGPILR"
     gene            complement(1576..2589)
                     /locus_tag="VFH39_03015"
     CDS             complement(1576..2589)
                     /locus_tag="VFH39_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6924772.1"
                     /translation="MFRSATFKLTLWYLAIITAISLCFSVVIYHFATGELQVGLHHQT
                     LRIYRNFPVFSGNPLLGNDPDVAAGSHHILWQLIYFNILVVVAAGIASYFLARRTLRP
                     IEQAHARQKRFTADVSHELRTPLTSLKMTSEVALMDSNASKQELRDALQSNLDEAGNM
                     SVLINNLLRLTRLDDAQTQATFAVHSSDPLITDVLKQLDRPIAAKQLTVTPPVQNVDL
                     YGDRDSLVQLLVILLDNAIKYGPAGSAVTMTAASNAGRSSISITDQGPGIGKEALPHV
                     FDRFYREDIARTNGATGGYGLGLSIAKLICDRHHGHITLTSRPGHGTTATVELPAGPP
                     VEA"
     gene            complement(2593..3273)
                     /locus_tag="VFH39_03020"
     CDS             complement(2593..3273)
                     /locus_tag="VFH39_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6924773.1"
                     /translation="MRILVVEDEVKIAQTIKKGLELKGYAVDAVYDGETGFSHAVDPD
                     YDLVVLDRMLPGMDGVELCRRARTEGVTTPILMLTARGTIGDKVEGLNSGADDYLVKP
                     FSFDELTARVRALLRRPAGHTGTVIKVGDLSLDTTTYEVRRGDDPIHLSQKEFALLEY
                     LMHHAGQVVTKDMIISHVWDGDADVLPNTVEVYIGYLRNKIDRPFTDRPPLLITMRGF
                     GYKLSDES"
BASE COUNT          582 a          962 c         1024 g          745 t
ORIGIN      
        1 cggggacggc gtgacattgt gttcgccatg ctgggcatca ctttcctgct gggcctcggc
       61 ttcctgggta tggagctgca tgagttccac cacatggtgc tggatggtaa cagctggcgc
      121 aagagcgctt tcctgtcgtc attcttcaca cttgtcggca cccacggggc gcacataacc
      181 tctgggctta tctggctgct ggccatggta ccgaaattag cccgtcaggg tctgacgccc
      241 gtgaacgtcc gtcgtctgac gctgttcagc ctgttctggc actttctgga cgtcatctgg
      301 atcttcatct ttaccatcgt gtacatgttc ggagtgatca agccatgaac aaacccgaga
      361 aaatcgtcgt cggcagccac gaggagccgc ctgtcaacct ggcgacatac atcatcggct
      421 acgtgctctc gctaggtttc acgctgacgg cctatgagct ggtggtgcac cgggtactca
      481 gcttcgcctg gctgttgacc gacatcatcg tgctggcctt cggtcagttt atcgtccaac
      541 tggtgtgctt tttgcacctg ggactggagc gcaaaccgcg ctggaagctg ctggtcttcg
      601 gcatgatgat ttccatcatc ctgatcatca tcatcggtac catctggatc atggccaatc
      661 tgaactacaa catgctgccg cgcaacctca accagtacat gcacacgaac gaagggctgt
      721 agtgaacctg cggggttact actcggtact gaagccgcag cggacactgt tcaacgtggt
      781 gacgacgctg gccggctacc tgctagcgac ccgctggcat attcattggt cggtgctgct
      841 gggggtgctg ggcggtacca cgctggtggt ggcttcggcc tgcgccgcca ataattgcac
      901 cgaccgcaac ctcgatgccc gcatgccgcg tacccgtaag cgcgtactcg tgaccaaaca
      961 agtctccgtg caacgggcgg cgtggttggg gttagtgctc gggatagccg gctttgtgtt
     1021 gcttggactg tatgtgaact ggctgaccgt cctgctcggc gccatcggct acatcgacta
     1081 cgtggtgctg tacgcctgga gtaaacggcg cacgccgcac agtacgctca tcgggaccgt
     1141 ctcgggcgcc gtgccgatca tggccggcta cacggcggcc accggtcggg tcgacctgac
     1201 ggcgctgctg ctggggctga ttctgaccgc ctggcagatg tcccacttct acgccatcgg
     1261 cattttccgc catgcggatt acgccgccgg caagctgccg atctggccgg tcgtcaaggg
     1321 agaccgcagc gcccgcgact ggatcatcac ctataccctg cttttcgtcc tctgttcggc
     1381 tgcgctgtgg gccgtaggag cggccggcgt tgtgtacctc gtatgcatga ctgctatagg
     1441 ggtagcgtgg ctctacgtgg ccgttgcggg cctcaagcgg cctgatctga tgcgctgggc
     1501 ccgtcaaatg ttcggctact ccctgttggt cattctggcc agctcgctgt tgatcgccgt
     1561 cggaccgata ctgcgctaag cttctaccgg tggtccggcg ggcagttcga ccgtggcggt
     1621 cgtgccatga cccggtcggc tggtcagcgt gatatgccca tggtggcggt cgcagatgag
     1681 cttggcgatg gacagaccga ggccgtagcc accggtggca ccgttggtac gggcgatgtc
     1741 ctcgcggtaa aaacggtcga agacgtgcgg caatgcctcc ttgccgatgc ccggcccctg
     1801 gtccgtgatg ctgatgctcg aacgacccgc gttgctcgcc gccgtcatag taacggcact
     1861 gccggccgga ccgtatttga tggcattatc cagcaggatg accagcagct gcaccaggct
     1921 gtcgcggtcg ccgtacagat cgacgttctg cacgggcggc gtgaccgtca gctgcttggc
     1981 ggcgatgggc cgatccagct gcttcagaac atcggtaatg agcgggtcgg agctatgtac
     2041 cgcgaaggtc gcctgggtct gggcatcgtc taaccgcgtc agccggagca gattgttgat
     2101 gagtacgctc atattgccgg cttcgtcgag gttgctttgc agggcgtcgc gcagttcctg
     2161 cttgctggca tttgagtcca taagtgccac ttcgctcgtc attttaagcg aagttagcgg
     2221 cgtgcgcagt tcatgcgata cgtcggccgt gaagcgcttt tgccgggcat gggcctgctc
     2281 gatcggtctt aaggtgcggc gagctagaaa ataactggcg attccggcgg ccacgactac
     2341 caggatgttg aagtaaataa gctgccagag gatgtgatgg ctgccggcgg caacgtccgg
     2401 atcattgccc agcagcgggt tgccgctgaa gaccgggaaa ttgcggtata tacgcagcgt
     2461 ctggtggtgt aggccgacct gcagttcgcc ggtcgcgaag tggtaaatga cgacgctgaa
     2521 gcacaggctg atggcggtta taatggccag gtaccagagc gtcagcttga acgtggcgga
     2581 gcggaacatc gcttagcttt catcgctcaa tttgtagccg aagccgcgca tggtgattag
     2641 caggggcggc cggtcggtga acggccggtc gatcttgttg cgcaagtaac cgatgtacac
     2701 ctcgacggtg ttcggcagga cgtcagcatc accgtcccac acgtggctga taatcatgtc
     2761 tttggtcacc acttgtccgg cgtggtgcat aaggtattcg agcagcgcga actccttttg
     2821 gctgaggtgg atcgggtcgt cgccgcgccg tacctcgtag gtggtcgtgt ccagtgatag
     2881 gtcgcctact tttatgaccg tgccggtatg gccggcgggt cgccgcagca gagcccgtac
     2941 ccgggccgtc agttcgtcga aactgaacgg cttgactaga taatcgtcgg caccgctgtt
     3001 caggccctcg actttgtcgc cgatggtacc ccgggcggtg agcattaaaa tgggcgtggt
     3061 gacgccttcg gtgcgtgccc gacggcacag ctcaaccccg tccatccccg gcagcatgcg
     3121 atccaacacc accaggtcgt aatccgggtc gaccgcatgg ctgaagccgg tttcgccatc
     3181 gtagacggca tcgacggcat agcctttcag ttccagccct ttcttaatag tctgggcgat
     3241 ctttacctcg tcttcgacga ctagtatcct catatgcctc cagtatacgt ccaaagttga
     3301 aaaacagctg tga
//
LOCUS       DASSFD010000130         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1294441, whole genome shotgun sequence.
ACCESSION   DASSFD010000130 DASSFD010000000
VERSION     DASSFD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294441"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..890
                     /locus_tag="VFH39_03025"
     CDS             93..890
                     /locus_tag="VFH39_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6924774.1"
                     /translation="MKLYSWNVNGIRAVLRKDALLPFIKKEQPDVLCLQETKAKDHQV
                     ELELPGYEQFWYSAEKPGYSGTAIFSKTKPLRVIDGFPQDIIKKFGVTGDTYGDPNLE
                     GRVIAAEFDKLWVVTVYTPNAKDDLSRIPLRHKHWDPAFLAYVKQLEKSKPVAFCGDL
                     NVAHTEDDLANPKPNRGKKGFTDEERAGFQAFVDAGFVDTLRLFKQGNGYYTWWSHFA
                     NARARNVGWRIDYFLISNSLKDKVKAADIHAEYMGSDHCPVSVTIDV"
     gene            887..1462
                     /locus_tag="VFH39_03030"
     CDS             887..1462
                     /locus_tag="VFH39_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6924775.1"
                     /translation="MTPKTVAIIFGFTEGEWHGKRFRRHLREAGYEVIKKAADADIII
                     AHSGGCYYVPRDLTTTQQVMLIDPPYWPARTIVRRGNNMLLHMTLQVRPGNEPFYQVH
                     KTARNIAYLVRHARTNLAMMRHARVFNLADNIAHPHTILVHNEEDPWLTPDLAHLKRI
                     NPYLRIHHLPGGHDDCWWHPEHYINLLQSDS"
     gene            1459..2376
                     /locus_tag="VFH39_03035"
     CDS             1459..2376
                     /locus_tag="VFH39_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6924776.1"
                     /translation="MTPDAYTIQETFIEVGDGHTLYVHDWGNKDAETPIIFLHGGPGG
                     QCGDKHKRQFDAATQRVIFFDQRGSGKSLPYGSLEHNTTQDMIHDIGAIADHFNIESF
                     FLTGRSWGSCLALAYALEHPERVKAMVLGGIFTGSQSEIDWLDNGVFATFYPEAWERY
                     LAATPESHRNNPGEYHYRRLMGTDEAAAKTSGLAYDTLEGSVMQLDDSFTPPSLEEYD
                     PAPIRIEMHYMHERCFLPDRFILKSAHKLAMPIYLVQGRYDMVCPPVTAYELNEYLPN
                     SELYMYIGGHRVEHESWNITKALLGRLTA"
     gene            complement(2510..2866)
                     /locus_tag="VFH39_03040"
     CDS             complement(2510..2866)
                     /locus_tag="VFH39_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924777.1"
                     /translation="MSERDQSKFASILTLLLGIWVAISPIWISVTGGAVASVIATGSV
                     IAVFSLIQYFWENTLPSWIVGLAAVWLFITTFAFGGVSGGAVVNLVISSVATFILAIW
                     DGAEVSISHQHHVGAV"
     gene            3039..>3256
                     /locus_tag="VFH39_03045"
     CDS             3039..>3256
                     /locus_tag="VFH39_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6924778.1"
                     /translation="MKVCTLALLVRNDSILLAMKKRGFGQGLWNGAGGKVEAGETIEQ
                     AMIRECQEEISVTPTTFEKVAVHDFVFP"
BASE COUNT          786 a          949 c          875 g          646 t
ORIGIN      
        1 taccagcacc tcggccacaa agttctctcg ggctacgagt ttcgctacta gccggcttgc
       61 aatcgcctgg cgcaacgcgc gacaataaga ccatgaaact ctattcatgg aacgtcaacg
      121 gtatacgcgc cgtgctgcgt aaggacgcgc tgctgccttt cataaaaaag gagcagcccg
      181 acgtcttgtg cctgcaagaa accaaggcca aagatcacca ggtcgagctg gagctaccgg
      241 gctatgagca gttctggtac agcgccgaga agcccggcta cagcggtacg gccatcttca
      301 gcaagaccaa gccgctacgg gtcatcgacg gttttccgca ggacatcatt aaaaagtttg
      361 gtgttactgg cgatacgtac ggtgatccga acctggaggg ccgggtcatt gccgccgaat
      421 tcgacaaact gtgggttgtc acggtgtata cgccgaacgc caaggacgac ttaagccgta
      481 tcccgctacg ccacaaacat tgggacccgg cgttcctggc gtacgtcaaa caattagaaa
      541 agtcgaaacc ggtcgcgttc tgcggcgacc tgaacgtggc gcacaccgag gacgacctgg
      601 ccaacccgaa gccgaaccgc ggcaagaagg gctttacgga cgaagaacgg gccggcttcc
      661 aagcattcgt cgatgccggc tttgtagaca cgctgcgcct gttcaagcag ggcaatggtt
      721 actacacctg gtggagccac ttcgccaacg cccgggctcg taacgtcggc tggcgtatcg
      781 actacttcct gattagcaac agcctgaagg ataaagtaaa agccgctgac atccacgctg
      841 agtacatggg tagcgaccat tgtccagtca gcgtcaccat cgacgtatga cgcctaaaac
      901 ggtcgcgatc atctttggct ttacagaagg cgagtggcac ggcaagcggt tccgccgtca
      961 tctgcgggag gccggctacg aggtgatcaa gaaagctgct gatgcggaca tcatcatcgc
     1021 ccactccggc ggctgctact acgtgccccg ggaccttacc accacacaac aggttatgct
     1081 catcgatcca ccgtactggc cggcccgtac catcgtccgc cgcgggaaca atatgctgct
     1141 gcacatgacg ttgcaggtcc ggccgggcaa cgaaccgttc tatcaggtac acaagacagc
     1201 ccgcaatatc gcctacctgg tgcgccacgc tcgcaccaat ctggccatga tgcgccacgc
     1261 ccgagtcttc aatttggcag acaatatcgc gcacccccat accatcttgg tgcataacga
     1321 agaagaccca tggctgaccc ccgatcttgc ccaccttaag cgcattaacc cgtacctacg
     1381 tatccaccac cttccgggcg gccatgacga ctgctggtgg caccctgagc attacatcaa
     1441 tctgttacaa tcagattcat gacgcctgat gcatacacca tccaggaaac cttcatagaa
     1501 gtcggcgacg gccacacctt gtacgtccac gattggggca acaaagatgc cgagacgccg
     1561 attatctttc tgcatggcgg cccgggcggc cagtgcgggg acaagcataa acgacagttc
     1621 gacgccgcga ctcagcgggt catattcttc gaccagcggg gcagcggcaa gagcctgccg
     1681 tacgggtcgc tggagcacaa caccacgcaa gacatgattc acgacatcgg tgcgattgcc
     1741 gatcacttca atattgaatc tttctttcta acgggccggt catggggttc ctgtttggcg
     1801 ctggcttacg ccctggaaca tccggagcgg gtgaaagcga tggtcctcgg agggatcttc
     1861 accggtagcc agtccgaaat cgattggctg gacaacggtg tcttcgccac cttctatccg
     1921 gaagcatggg agcggtactt agcagctacg cccgagtcgc atcgtaataa tccgggcgaa
     1981 taccattacc gacgcctcat gggtacggac gaggcggcag ccaaaacctc aggtctggcg
     2041 tacgacaccc tggaaggcag cgtcatgcag ctcgacgaca gttttacacc cccgagtctc
     2101 gaggaatacg atccggcccc tatccgtatc gaaatgcact atatgcatga gcgctgcttc
     2161 ttgccagacc gcttcatact caagagcgcc cataagcttg ccatgccgat ttatctggtg
     2221 caggggcgat acgacatggt ctgcccgccg gttacggcct acgagcttaa cgagtatctt
     2281 ccgaacagcg aattgtatat gtacattggc ggccaccggg tggagcacga aagctggaac
     2341 attaccaaag cgctgcttgg acggctgact gcctgatgcc gaggaactca gcttctaccc
     2401 acaagaaaat ctcataaaaa agaaaggcag tggaccggaa tagcgcaaca ttccggccac
     2461 tgctaaccta gccttgtgca aggctattag tatacattcc ggcgcccgac tagactgcgc
     2521 ccacgtggtg ctggtggctg atggacactt cggcgccatc ccaaatagct aatatgaatg
     2581 tagctaccga ggatatcacg agattgacga ccgcgccgcc gctcaccccg ccaaacgcga
     2641 aggtagtaat gaacagccac acggctgcta agccgactat ccagctcggt aatgtgtttt
     2701 cccagaagta ctggattagg ctgaagaccg ctatgacact accggtggcg attacgctcg
     2761 ctaccgcgcc gccggtcacc gatatccaga tgggcgaaat ggcgacccat atgcccagta
     2821 acaacgtcag aatgcttgcg aatttggatt gatcacgttc gctcatggta taactcctta
     2881 cttaagtatt tagccgcaca tttacagatg tacgcgtgaa cctcctttct acatccagaa
     2941 tcctcccgtt gagctgaaat ggcaggaata ttttttacaa tagagagccg tggcgcgcag
     3001 ccaggcgagc ggagcgatat aactgctaca atacctgcat gaaagtgtgt accctggcgc
     3061 tattggtgcg caatgacagt attctgctgg ccatgaagaa acgcggcttc ggccagggat
     3121 tatggaacgg tgccggcggc aaggtcgagg caggcgagac catcgagcag gcgatgatcc
     3181 gcgaatgcca ggaggagata agcgtgacac cgaccacgtt cgagaaggtt gccgttcacg
     3241 atttcgtctt tcctga
//
LOCUS       DASSFD010000131         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_405260, whole genome shotgun sequence.
ACCESSION   DASSFD010000131 DASSFD010000000
VERSION     DASSFD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405260"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFH39_03050"
     CDS             complement(<1..280)
                     /locus_tag="VFH39_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HET6924779.1"
                     /translation="MGSSLWQAVLGEIELSVSRGNFVTWFKNTRLVQQTDERVVIGVP
                     NVFIKQQLERKYNDLVTETLKKNGVEPEAIEYKIVSVTPVQRVDDEPVL"
     gene            632..769
                     /gene="rpmH"
                     /locus_tag="VFH39_03055"
     CDS             632..769
                     /gene="rpmH"
                     /locus_tag="VFH39_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET6924780.1"
                     /translation="MPKRTYQPKKRQRSREHGFMKRMASKAGRNVLARRRLKGRQRLT
                     A"
     gene            complement(1007..1408)
                     /locus_tag="VFH39_03060"
     CDS             complement(1007..1408)
                     /locus_tag="VFH39_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924781.1"
                     /translation="MACLKGSGGKQESGVKQQGSAKAGGFGPFNTSAKPAASVSRFPV
                     PGRLDGFGFHKQAAHLTLKLREGHRSSLVAKYRKHKVVRLAYVAGLCPERLKHPAADA
                     VAGNGRLVHLAADHHGRPESAAPLVANKFQT"
     gene            1442..2440
                     /locus_tag="VFH39_03065"
     CDS             1442..2440
                     /locus_tag="VFH39_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HET6924782.1"
                     /translation="MFTTIIVKPIFNLLVLIYALLPGHNLGLAIIIFTIIVRLLLWPL
                     LKKQLHQAKAMRALQPEIKRVKQEAKGDRQKESRLLMELYKERGINPVGSIGVLIPQF
                     IVLIGLYSGLRRVVDDPHAIIDFAYPILQHLGWMQHVAHNIHAFDQTLFGVVDLTRAA
                     TGKAGIYWPAMVLVLASAIMQYYQSKQLMPSDKNARSLRQILASAGEGKQADQAEVSA
                     AVGRSTRYLLPVMIFLVTVNLPAALGLYWLVGGIVAFIQQDRVLKRDESEMEAIADRG
                     SPNQSGEANDSAKGKPGKDVAAIPEAEIVTQGPRSKKQETSKRHKSSKKAKRRKKR"
     gene            2437..2955
                     /locus_tag="VFH39_03070"
     CDS             2437..2955
                     /locus_tag="VFH39_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET6924783.1"
                     /translation="MSEGLSEDTNTTGEAAMANDSLEEAILYAKKYLEDLLSFFGLNT
                     DVHATSDDEVIQLNVPSTHLNGFLIGHNGENMRSLQYLVSTALKNNRFAAHRVNVDIA
                     EYKKQRADRLRDRAETWVKQVKDSGQPMDLMPMNAADRRVVHQLAGEYGLSTHSVGEG
                     RDRHIVLEPTEE"
     gene            3023..>3567
                     /locus_tag="VFH39_03075"
     CDS             3023..>3567
                     /locus_tag="VFH39_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6924784.1"
                     /translation="MNETSLRQLLADEESYGLEFKAARDSYSRPKLNDYCAALANENG
                     GHLLLGVNNAKRIVGTNAFSGNWNRLAHEITNELQLRIKVYEIKVSEGRVLAFEVPRH
                     ITGTPVQVKGGTGKYRYPIRNGESLEEMDQETLKQIFAERSEDWSAQTIENAKFTDLS
                     GTAMQLYRNRWAEHTKDPARR"
BASE COUNT          862 a         1012 c          958 g          735 t
ORIGIN      
        1 ccagcaccgg ttcatcgtcg acgcgttgca ccggcgtgac cgatactatt ttgtattcta
       61 tagcctccgg ttcgacaccg ttctttttga gtgtttcggt gacaaggtcg ttgtatttgc
      121 gctccagctg ctgctttatg aacacattcg gcacccctat gacgacccgc tcatccgtct
      181 gttgtacgag ccgggtgttc ttaaaccacg ttacgaaatt acctctggaa accgacagtt
      241 caatctcgcc aagtaccgcc tgccacagag aagagcccat gcagcgttca cccctttgcc
      301 gttaccttac ccttgttagt accacgtatt gtacccaatt gccggctact tttccacacc
      361 ccgggcacag caggggaggg agcaggcgcc gcgccggcta aaccggggta gagttataca
      421 cagccgttat ttacccctgt gaaattgtgg ataattcttg gcaatctgcg gaaaagttgt
      481 tagaattagt ggactaaaca ttgtcaattt cacgctattc gcgtgaagca atcagcaacc
      541 aggaactagc agctagcact tggaaacacg caattcgcgg accggctatt ttctaattcc
      601 tatttactaa ctactaatcc gaaggatttt tatgcccaaa cgaacctacc aacccaaaaa
      661 acgccagcgc tcgcgcgagc acggcttcat gaagcgcatg gccagcaaag ccggtcgtaa
      721 cgttttggct cgccgccgtc taaagggccg ccaacgcctg acggcgtaga aactagtagc
      781 taggaaatag taactggcgg gaccgtggtg gcttgcacgc aagggatttt ctggtggtcg
      841 cggacggccg tttcctagtt gcttactact aattcctgat cgctatttcc taattcctag
      901 ttactagcta ctattttgat atatgctatc caaaatcaat cggtttcatg ggctgggcgg
      961 cctgaacttt gcctataagc aaggccaaac tgtccgcggg ccgctcttaa gtttgaaatt
     1021 tgttcgcaac caaaggcgcg gcactttccg ggcggccgtg gtggtcggcc gcaaggtgca
     1081 caagtcggcc gttacccgca accgcatccg ccgccgggtg tttgaggcgc tccgggcaca
     1141 ggccggcgac atacgcgagc cgtacgacct tgtgtttacg gtatttggcg accaggctgc
     1201 tacggtgccc ttcgcggagc ttgagcgtca ggtgcgcagc ctgcttatga aagccgaagc
     1261 cgtccaaacg accgggaacc gggaaccggg aaacagaggc ggccggcttc gctgaggtat
     1321 taaacggtcc aaaaccgccg gctttcgcgc tgccctgctg cttgactcct gattcttgtt
     1381 taccgccgct tcctttcagg cacgccatgc tatagtagta ccgaaggaaa accgatacgt
     1441 tatgttcact accatcatcg taaagccgat ctttaacctg ctggtgctga tatacgccct
     1501 gctgcccggc cacaacctcg gtttggcgat cattatcttc accataattg tccgcctgct
     1561 actgtggccg ctcctgaaaa agcagctgca ccaggccaag gccatgcgtg ctttgcagcc
     1621 ggagatcaaa cgcgttaaac aggaagccaa aggtgaccgg cagaaagaat cgcgcctgct
     1681 catggagctc tacaaagagc gcggcatcaa cccggtcggc tccatcggcg tgctcatacc
     1741 gcagttcatt gtgctgatag gcctctactc ggggttgcgc cgggtggttg atgaccccca
     1801 cgccatcatt gacttcgcct accccattct gcagcacctg ggttggatgc agcacgtggc
     1861 gcacaacatc cacgcttttg accagaccct gttcggagtt gttgacctga cgcgcgccgc
     1921 taccggcaag gccggcatct actggccggc catggtgctg gtactggcca gcgccatcat
     1981 gcagtactac cagagcaagc agctgatgcc gagcgacaag aacgcccgca gtttgcgtca
     2041 gatcctggcg tctgccggcg aaggcaagca ggcggaccag gccgaagtga gcgccgccgt
     2101 cggccgctct acccgatact tgctgccggt gatgatattc ctggtcaccg taaatctgcc
     2161 ggccgctctg ggcctgtact ggctggtcgg cggtatcgtc gccttcatcc agcaggaccg
     2221 cgtgcttaag cgcgacgaaa gtgaaatgga agccatagcc gacagggggt ccccgaacca
     2281 atccggcgaa gcgaatgatt cggccaaagg caagcccggt aaagatgtcg ccgccattcc
     2341 cgaagccgaa atcgtcactc aaggaccaag aagcaagaag caagaaacaa gcaaaaggca
     2401 caaatcctct aaaaaagcta agcggaggaa gaagagatga gtgaaggcct gagcgaagac
     2461 acgaatacga cgggcgaggc cgctatggcc aatgactccc tggaagaagc tatcctgtat
     2521 gcaaagaaat acctggaaga cctgctgtcc ttcttcggtc tcaacactga cgtccacgcc
     2581 accagcgacg acgaagttat tcagctgaac gtgccgagca cgcacctgaa cggcttcctt
     2641 ataggccata acggcgagaa catgcgctcg ttgcaatacc tggtaagcac cgcgctcaaa
     2701 aacaaccgtt ttgccgccca ccgggtaaat gtggacattg ccgagtacaa aaagcagcgt
     2761 gccgaccgtt tgcgcgaccg cgccgaaacc tgggttaagc aggtcaaaga ctcgggccag
     2821 cccatggacc tcatgcccat gaatgccgcc gaccgccggg tggtgcacca gctggccggc
     2881 gagtacggtc tgtccaccca ctctgtcggc gaaggccgcg accgccacat cgtgctcgag
     2941 cccacagaag aatagccccg gtaccttgtg gtcgcctact gactgcagta aaattaagcg
     3001 acgaattata gggtaagcca aggtgaacga aacatcacta cgccagctac ttgctgatga
     3061 agaaagttat gggcttgaat ttaaggcggc tcgcgattcc tatagcaggc ccaaacttaa
     3121 tgactactgc gcggcacttg caaacgagaa tggcggccat ttactgctgg gagtgaataa
     3181 cgcaaagcgg atagtgggca caaatgcttt cagcggcaac tggaaccgtc tggcccacga
     3241 aattacgaac gaactgcaat tgcgcatcaa agtttatgaa attaaggtga gtgaagggcg
     3301 tgttctggcc tttgaggtgc ccaggcatat aacaggaacg ccagtccagg ttaagggcgg
     3361 cacaggcaag tatcgttacc cgatacgcaa tggcgagtcc ctggaggaaa tggatcagga
     3421 aacgctcaaa cagatattcg ctgagcgttc cgaggactgg agtgcacaga ccatagagaa
     3481 tgccaaattt accgacctta gcgggaccgc tatgcagcta tacaggaata gatgggctga
     3541 gcacaccaaa gatccggcgc gccgtaa
//
LOCUS       DASSFD010000132         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_680189, whole genome shotgun sequence.
ACCESSION   DASSFD010000132 DASSFD010000000
VERSION     DASSFD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680189"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VFH39_03080"
     CDS             complement(<1..1628)
                     /locus_tag="VFH39_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6924785.1"
                     /translation="MSFKVIRRTAYKWPKRILKNLANQDRDSRYVFVLIAGGLIFLLA
                     TLQALSQGLFDSVERPVFQFFNGWPHLLFNPMYAVTQLGSLASLVIWCGLAWYLINRR
                     AAKTVFAAGLAAWLLAKVAKYAVHRGRPEALLPHIHLFSHETFTGFGFPSGHSTLSAA
                     CATVLYYQVKPRYRKYLLLAVLLVGTSRMYLGAHFPFDVIGGWALGMAIGAATVLIMG
                     TSANRLSVQRLKKILRRKGYEPHSLTFADVDARGSRPFFLEDTSGTKYFAKIFGKQEH
                     AADWLFKIYRFFRFKNLQGEEPYLNSKRNVELEALATLWAANTGARVAKVADLVQAGS
                     LWILMQERIDGKPLSDHKRIKDTTLVDAWRQVEKLHAGRLAHRDLRAANLLIDKQGKV
                     WVIDFGFAEVSARPRRMYMDVAELLMSMSLVVGVERTLKAAWRIVPHDKLEAVLPYLQ
                     KAVFSGATSKLLKQNPELLDELKASLRHKLGVKEDEVEEADIVRLNTGKVVNIVILAA
                     FLYLIVPQFKQFSGALHSLSHITPGWLVLVAISSA"
     gene            1779..1940
                     /locus_tag="VFH39_03085"
     CDS             1779..1940
                     /locus_tag="VFH39_03085"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET6924786.1"
                     /translation="MNMLLTIALIILALWILGFAIHIGGALIHLLLIIGVILLIAHLL
                     RGRRRRTIV"
     gene            1967..2368
                     /locus_tag="VFH39_03090"
     CDS             1967..2368
                     /locus_tag="VFH39_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924787.1"
                     /translation="MTAHTERITRETTPDDRVIERREIEHEVPGDAYTSGRTNLAQRI
                     IWFLAGILLLLLVFRFVLALLGANPSNWFANFIYTTSHPFVAPFFGLFGYHLQYGVSR
                     FEIYTLVAIAVYVVIAWGLDRLVTIHRPRTY"
     gene            complement(2365..>3192)
                     /locus_tag="VFH39_03095"
     CDS             complement(2365..>3192)
                     /locus_tag="VFH39_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924788.1"
                     /translation="DDKGRKQYIYHPKWRTMRDLIKFYRMLIFGKALPDIRQDIERAL
                     GRSKLDKPKLIGAMLWLLDNTYIRVGNEQYYEQNESVGLTTLTRQHVIVAGPVATLSF
                     KGKSGKMQQITFEDQRIAKVLEELRKRPGERLFSYREGGTDHMLEPNDINRYLHELTG
                     VTVSAKDFRTWGGTLMAFNHLIEAERSKETTPKPEKVVIEAVDAAADVLGNTRSVARS
                     SYVHPHILAAYGSRNFKHYYDTAARCHKLPGLDKRETELLSFLEQLFEDEFDLLKQA"
BASE COUNT          678 a          856 c          921 g          737 t
ORIGIN      
        1 gccgaactaa tcgccaccag cacgagccag ccaggggtga tgtgcgacaa cgaatgcagt
       61 gctccgctga attgcttgaa ctgcgggacg ataagataca gaaaagccgc caaaatgacg
      121 atattgacga cctttccggt attaaggcgc acgatgtcgg cttcttcgac ctcgtcttct
      181 ttgacgccga gcttgtgacg caaacttgcc ttaagctcat ccagcagctc cggattttgc
      241 ttgagtaact tgctggtggc gccgctgaag acggctttct gcaagtaggg aagcactgct
      301 tccagtttgt cgtgcggtac gatccgccac gcagctttca aagtacgttc cacccccact
      361 accagtgaca tcgacataag cagctcagcg acatccatgt acatacggcg gggtcgggcg
      421 gaaacttcgg caaacccgaa atcgatgacc cagaccttgc cttgcttatc gatgagcaga
      481 ttggcggccc gcagatcccg gtgggccagt cggccggcat gcagtttttc gacctggcgc
      541 caggcgtcga cgagcgtcgt gtctttgatg cgtttatgat ccgagagggg tttgccgtcg
      601 atacgttcct gcatcaatat ccatagcgag ccggcttgga ccaggtcggc taccttggcc
      661 acccgggcac cggtattggc cgcccacaag gtagccagcg cttcgagctc gacattgcgc
      721 ttgctgttga ggtatggttc ctctccttgc aggtttttga agcggaagaa acgatagatt
      781 ttgaacaacc agtcggcggc gtgctcctgt ttgccgaata tcttggcgaa gtacttggtg
      841 ccggaagtgt cctctaagaa gaacgggcgc gaaccgcggg catcgacgtc ggcgaacgta
      901 aggctgtggg gttcatagcc tttgcggcgc agaattttct tgagccgttg tacagacaac
      961 cggtttgcgg aggtgcccat aatgagtacc gtcgccgcgc cgatagccat gcccagggcc
     1021 cagccgccga taacatcgaa ggggaagtgc gccccaaggt acatacggct ggtaccgacc
     1081 agcagaacgg ccaacagcag gtacttacga taacggggtt ttacctgata gtacagtacc
     1141 gtggcacagg cggccgacag ggtggaatgc cccgacggaa agccgaagcc ggtgaaagtc
     1201 tcatggctga acaggtggat atgcggcagg agtgcttccg gacggccacg gtgcacagcg
     1261 tatttagcta ccttggccag cagccaggcg gccaggccgg ccgcaaagac cgtcttggct
     1321 gctcgtcggt ttatgaggta ccaagccaga ccgcaccaga tgacgagtga cgccaagcta
     1381 cccagctggg taacggcgta catcgggttg aagagtaggt gcggccagcc gttgaagaac
     1441 tggaataccg gtcgctcgac actgtcgaac aacccctggg agagcgcctg tagcgtggca
     1501 agcaagaaaa tcaggccgcc ggcgatgagc acgaagacat agcgggagtc ccggtcctgg
     1561 ttggcgagat ttttgagtat ccgtttcggc catttataag ccgtgcggcg gatgaccttg
     1621 aatgacataa ggacatattt tactgttttt atcaaaatat tgcaaaaagc ttcatcggtt
     1681 tcatcagatt gaagcatctt agcgcaacgt caaatatttg acagtactgc cgctacgcta
     1741 cggtgtatgc tctgtagcag cgtaatcaac aaggagacat gaatatgctg ttaacgatcg
     1801 cacttatcat tttggcttta tggattctgg gcttcgccat tcacatcggg ggcgcgctca
     1861 tccatctgtt gcttatcatc ggcgtgatac tactgatcgc ccacctgctt cgagggcgtc
     1921 gacgccgcac gatagtttaa ctaagaaata gtaaaggaga gtgatcatga ccgcccacac
     1981 cgaaagaatt acccgcgaga ctacgcccga cgaccgagtg attgaacggc gtgaaattga
     2041 acacgaagta cctggcgacg cctatacttc tggccgcacg aacctcgccc agcgcatcat
     2101 ctggttcctg gccggcattt tgctgttact gctggtcttc cgcttcgtat tggcactgct
     2161 gggcgccaac ccgagcaact ggtttgccaa cttcatctac accacgagcc acccgttcgt
     2221 agctccattc ttcggattgt tcggctacca tctgcaatat ggcgtgtccc gcttcgaaat
     2281 ttacaccttg gtcgccatag cggtgtatgt ggtgattgcc tggggtttgg atcgtctggt
     2341 gactattcac cggccgcgga catattaagc ctgtttgagt agatcgaact cgtcttcgaa
     2401 taactgctcc aagaagctca gtagctcggt ttcccgcttg tccagacctg gtaacttgtg
     2461 gcatcgggcg gctgtgtcgt agtagtgctt gaagttgcgg ctgccgtacg cggccaagat
     2521 gtgcggatgc acgtagctgc tgcgtgcgac ggagcgcgta ttgccaagca catcggcggc
     2581 ggcatcaacc gcttcgatga cgaccttttc gggcttcgga gtggtttctt tggagcgctc
     2641 ggcctcaatc aggtggttga atgccatgag cgtgccgccc caggtccgaa aatcctttgc
     2701 cgatacagtg acgccggtca gctcgtgcag gtaacggttg atatcgttcg gctccagcat
     2761 atgatcggtt ccgccttcgc ggtagctgaa caaccgctcg cctggccgct ttcgtaattc
     2821 ttccaggacc ttagcgatgc gttggtcttc gaacgtgatt tgctgcatct tgccggactt
     2881 acctttgaat gatagcgtag cgaccgggcc ggcaacgatg acatgctggc gcgtgagggt
     2941 agtgaggccg acggactcat tctgttcata atactgctcg ttgccgacgc ggatgtaggt
     3001 attgtcgagc agccagagca tggcgccgat gagcttgggt ttgtcgagct tggagcggcc
     3061 cagcgcacgt tcgatgtcct gccgtatgtc cggtaaggcc ttcccgaaaa tcagcatgcg
     3121 gtagaacttg atgaggtcgc gcatggtccg ccacttgggg tgataaatgt attgtttgcg
     3181 gcctttgtcg tc
//
LOCUS       DASSFD010000133         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_839112, whole genome shotgun sequence.
ACCESSION   DASSFD010000133 DASSFD010000000
VERSION     DASSFD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839112"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1030
                     /locus_tag="VFH39_03100"
     CDS             137..1030
                     /locus_tag="VFH39_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924789.1"
                     /translation="MNTGGDPHGGFTIVETLIVLAVTGFLFVSIAGLISGRQGKTEFQ
                     TAINGLQQQIQQIINETESGYYPNGVDYSCTAGLGLPPTISTGTPGKGQGANSSCIFL
                     GKVLQFGPSDAAYKNPEKYLVWPIAGNQNLAGAGSDGLLGAGDQTLAKSYPTPIVPIR
                     SSSALQSGLTVDHMTYDNGAPGVPTAGVGFMVGNGDANGTFAGSTSDGLGAGSLPLSL
                     YAIKSTQVGTSSANDMQAALSSASGSPLRYTPVDSVDICVNSGTTHQSGLITIGSSSS
                     ADGVKRQLTVTLKIYGAPQCA"
     gene            1069..1443
                     /locus_tag="VFH39_03105"
     CDS             1069..1443
                     /locus_tag="VFH39_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924790.1"
                     /translation="MIAIAIVSLVLVTAYASANRNVGALQDSQEHSEALKLVQAQTEF
                     LRARGAPGGGNTCFASDGTVTGNCTFNSDGSYSASGRYKLKIQQGGGDCAADAYTVSA
                     TWDSLLGANAQVTVCYRPSEGS"
     gene            1447..2160
                     /locus_tag="VFH39_03110"
     CDS             1447..2160
                     /locus_tag="VFH39_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924791.1"
                     /translation="MARDIKLRPAARRGLAAGFTIVELMIATLVFSMVLLVVTYGVLH
                     FTHDYYQGVNSSTTQGVARNVADTLSQAIQFGGSTPIGSPSGSTSGYLCAGNVEFNYR
                     LDKTVGNSGSWALLRFPITGTCYTGAVPNAPAGTKPTEMLQPRMRLLKFDVSPVSAGS
                     LLYKVDIGIALGEDDLLCDTKVTPPTSTGGCDTSAASLGALPLPGDADSIRCKASSGA
                     AQFCDVALLSTVAQTRLTP"
     gene            2187..>2504
                     /locus_tag="VFH39_03115"
     CDS             2187..>2504
                     /locus_tag="VFH39_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HET6924792.1"
                     /translation="MNKQQSIRGDERGMVSIMVTLVMIIVITLIVIGFAQVVRRNQRE
                     TLDRQLSTQAYYAAETGINDAYKAMFIDSSPQNFSFQPSYKTDCSAFMNDQGLNNVLN
                     NAAG"
BASE COUNT          528 a          709 c          763 g          504 t
ORIGIN      
        1 tacctgctgg ccggcacgct ggtggtggtg ctgttcgggc aggcgatcct cgattggtat
       61 accggtctgc tacctctgta aaatcccggt acggtacagg ggcttgcatc gaagcgctta
      121 tggtatactt aggctcatga atacgggggg cgacccgcac ggcggcttta ccattgttga
      181 gacgttgata gtgctcgccg tgaccggctt cttattcgtc agcattgccg ggctgatcag
      241 cggccggcag ggcaagaccg agttccagac ggccataaat ggcttgcaac agcagattca
      301 acagataatc aacgagacag agagcggcta ctaccccaac ggtgtcgact attcatgtac
      361 cgctggcctg gggctaccgc cgaccatcag taccggtacg ccagggaagg ggcagggtgc
      421 gaactcgagc tgtatattcc tcggcaaggt tctgcagttc ggcccttctg acgctgccta
      481 taagaacccc gagaagtacc tggtatggcc gattgccggc aaccagaatc tggccggcgc
      541 aggcagtgac ggattactcg gcgcaggcga tcagacgctg gcgaagtcat acccgacacc
      601 cattgtgccg atccgctcca gttctgcatt gcaaagcggc ctgacggtcg accatatgac
      661 ctacgacaac ggagctcctg gggtgcccac tgccggtgtg ggcttcatgg tcggtaatgg
      721 ggatgccaac ggtacgttcg ccggttccac ttctgacggg ctgggcgccg gttccttgcc
      781 gctgagcctc tatgccatca agtcgaccca ggtcgggaca agttctgcta acgatatgca
      841 ggcggcgctg agctcggctt cgggatcacc tttgcgttac acgccggttg attcggtgga
      901 tatctgcgtc aacagcggta cgacccatca gtccggcctg ataaccatcg gtagcagctc
      961 gtctgccgac ggagtcaaac gccagctgac cgtgacgctg aagatttacg gagctccgca
     1021 atgcgcatag gccgtttacg agattcggga gatacgatca tagaggtatt gatagccatt
     1081 gccatcgtca gcttggtgct ggtcaccgcg tacgcctcgg ctaaccgtaa tgtcggagcg
     1141 ctgcaggaca gccaggaaca ttccgaagcg ctgaagctcg tacaggccca gacggaattc
     1201 ctgcgcgccc gtggcgcgcc ggggggcggc aatacgtgct ttgcatcgga cggtacggtg
     1261 accggcaact gcacgtttaa ctccgatggc agctatagcg ccagcggccg gtacaagctc
     1321 aaaatacaac agggcggcgg cgattgtgcg gctgacgcct acacggtgag tgcaacctgg
     1381 gatagtctgc tgggtgcgaa tgcgcaggtg acagtgtgtt accgaccgtc ggagggcagc
     1441 tagatcatgg cgcgagacat aaagttacgt cctgcggcgc ggcgcggcct ggcggcaggc
     1501 ttcaccatcg tcgagctcat gatagcgacg ctggtgtttt cgatggtgct cctcgtagtg
     1561 acctatggcg tactgcactt cacgcatgat tactaccagg gcgtgaatag ctcgaccacg
     1621 cagggggtcg cgcggaatgt agccgatacg ctctcgcagg ccatacagtt cggtggctcc
     1681 acaccgatag gcagcccttc cggaagtact tccggctatc tgtgtgccgg caatgtggag
     1741 ttcaattatc gtctggataa gaccgttggc aacagcggtt cctgggcact gttacgattc
     1801 cccatcacag ggacatgcta caccggcgcg gtgccgaacg ccccagccgg gacgaaaccg
     1861 accgaaatgc tgcagcctcg gatgcggctc ttgaagttcg atgtcagccc ggtcagcgcc
     1921 ggatcactgc tgtacaaagt cgatatcggt atcgctctcg gcgaagatga tttgctatgc
     1981 gacacgaaag taacgccccc caccagtacc ggtggttgcg acacgagtgc ggcttcattg
     2041 ggtgcattac cgctgccggg tgatgcggac agtatccgct gcaaggccag ctccggggca
     2101 gctcagttct gtgatgtggc gctgctcagc acggtcgccc agacaaggct gacgccgtga
     2161 acaaggtgta caataaagct aacggtatga acaaacagca gagtatccga ggggatgagc
     2221 gggggatggt gtccataatg gtgacactgg tgatgatcat cgtcattacg ctcatcgtca
     2281 tcggttttgc gcaggtggtc cggcgcaacc agcgtgagac gctcgaccgc caactcagca
     2341 cgcaggcgta ctacgctgcc gagaccggca taaatgacgc ctacaaagcg atgttcatcg
     2401 acagcagccc gcagaacttc tcgtttcagc cgtcatacaa gacggactgc tcggcattca
     2461 tgaatgacca gggcctgaat aacgtgctca ataacgcagc cggc
//
LOCUS       DASSFD010000134        11331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1165116, whole genome shotgun sequence.
ACCESSION   DASSFD010000134 DASSFD010000000
VERSION     DASSFD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165116"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFH39_03120"
     CDS             complement(<1..530)
                     /locus_tag="VFH39_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HET6924793.1"
                     /translation="MHALIICVVIVLLVLLSAICSGLNVSLMALDPADLRRKSKLGDS
                     NARKVRRFRRNTHLSLAAILLTNVAAVSATSLVLESVFFGVIAGLLGTLLIVIFGEIF
                     PQALFTRRALAFTARFSPLLRLMIIVTYPVSKPLQLLLDRLFQAEETHLESRQELGIM
                     ISEHIGSSQSELDDDEV"
     gene            671..1006
                     /locus_tag="VFH39_03125"
     CDS             671..1006
                     /locus_tag="VFH39_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET6924794.1"
                     /translation="MEDTMPVVTTNDLPGYEVEEVYGEVFGLIVRSRNVFSNFGASLR
                     TIVGGEVKGYTALLADSREHALQRLRAAAAEKGANAVLTMRFDSGDIGGVMNEVAAYG
                     TAVRVRKKA"
     gene            1011..1589
                     /locus_tag="VFH39_03130"
     CDS             1011..1589
                     /locus_tag="VFH39_03130"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6924795.1"
                     /translation="MDELARLLSGPAETVARRLLGCELVRELDGQRLVGRIVETEAYD
                     ETDPASHSYRGRTNRTAVMFGPPGHLYVYFTYGMHYCMNVVTGDDGHGSGVLLRALEP
                     LAGEAVMRRNRHDRSDPSVTNGPAKLCEALAVDSSLNGHDLRQAPLKLVVRPPMAGQD
                     IVVATRVGISQAKDKPWRFYVADNRHVSRVRV"
     gene            complement(1712..2887)
                     /gene="cysS"
                     /locus_tag="VFH39_03135"
     CDS             complement(1712..2887)
                     /gene="cysS"
                     /locus_tag="VFH39_03135"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6924796.1"
                     /translation="MHLYNTASRRLETSTWARDVHIYVCGITPYDAAHMGHIFTFVTY
                     DILSRYLEYQGHRVKLVRNITDVDEPIYTRAQELGIPYMQLARDETAHFQEVLQQLDF
                     LPAFAEPRASDYIEAMAAAVKRLLDNGHAYRLANGDIYFDVSTFPRFGKQSGFTRRLQ
                     TAFMASRGGDPERLDKRHPFDFLLWRAVPDTDDPARWESVVGTGRPGWHIECSVMSSD
                     LLGTPIDIHGGGMDLIFPHHEAETAQSESLSAYPFVRHWMHVAPLYCYGEKMSKSLGN
                     LVFAGDLLAHHDPAVIRLALLQYHYRIGGEWHGDVLTKAKEQLGLFKRALATRSGPSP
                     ALYLEHFFSAMDDDLDTPEALHVLHHLAWDTVKKGGSDSQAPAKLAELLGLLGIQLA"
     gene            2961..3686
                     /locus_tag="VFH39_03140"
     CDS             2961..3686
                     /locus_tag="VFH39_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET6924797.1"
                     /translation="MASVQPYDPKIVLAIGAHADDIDFGASGSVAAWVAKGAEVHYLV
                     ITDGSKGSADTGMATEQLVAMRQQEQRDAAAVLGVKEVHFLGYEDGMLEVTMDLKKDI
                     VRLIRQIRPDTVVVMDPTTVYYADYNFINHPDHRAAGQATLDAVFPLARDHLSFPELY
                     LGEKLEPHKVAHVLLINLEKQNYYVDISATFDTKVQALQRHASQVPSVQALTEMLQQR
                     AAALGRKTGSQYAEGFVRIDTQA"
     gene            3735..4463
                     /locus_tag="VFH39_03145"
     CDS             3735..4463
                     /locus_tag="VFH39_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET6924798.1"
                     /translation="MLAILLAAATFVSTLLGGLTALKNRDRLHRFLGFAAGVLLGVVA
                     FDLLPEIFSRLQELHHTPTGAMIALVGGFLLFHIVEKSILIHHSQEAEYETHHHPDVG
                     VASALALIGHSFLDGVGIGLGFKAGTAVGLTVALAVIAHDFTDGLNTVNLMLLNRNHS
                     RQAVTFLVLDALAPIVGVLSTLWFTISSNLLVLYLGFFAGFLLYIGASEILPEAHSKH
                     SSYATIALTILGAVFIFVVTRVAI"
     gene            4492..5550
                     /locus_tag="VFH39_03150"
     CDS             4492..5550
                     /locus_tag="VFH39_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET6924799.1"
                     /translation="MLAAVIVLGVLVVALLLVVFRQQLTPRHDGNSALLIKEDLKTLS
                     EDITKLKDGLQQQLSDQLGASSKQMSAQFQASAKIIRDVTEKLTELDRTNKNVGDIAS
                     ELKLLQNVLQNPKQRGVLGEYYLEQILKNLLPPGSYELQYKLGEGLIVDAVIKLDDKV
                     LPIDSKFSLENYNRLLEAADGDRDILERAFKQDLKQRIEETSKYIKPGKGTLDQALMF
                     IPSEAIYYDLLANKVGLGNVSGRNLMQYAAADKHVTIVGPSTLSAMLQTIVQGLRSLE
                     IHKDTEKIRQNIEQLGKHLIAHNAYMQKLGNSLGTTVGHFNTTYKELGKIDKDIVKIT
                     SSEPHVEPMALERPSLDD"
     gene            5601..5894
                     /locus_tag="VFH39_03155"
     CDS             5601..5894
                     /locus_tag="VFH39_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924800.1"
                     /translation="MAKAELAPPSNRLFIPGSEAVEVGGVRRIGPNSVRRVERAAHHY
                     MKYRDQFKRRGAFILCSGGYSGSAHGQEAPVDRSQREARLLSNCEMSTTFPHT"
     gene            5891..6238
                     /locus_tag="VFH39_03160"
     CDS             5891..6238
                     /locus_tag="VFH39_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924801.1"
                     /translation="MIEEEDESTSAFSNVANSILGGWVEPTAFNAEDRLGVVAPPLFQ
                     KRLQIIWSKVNIGREAIEPICDNENMSFATELAGKLITRYLLRSATEPADLLQAEARF
                     NQLPIAGRSGNTA"
     gene            complement(6267..7748)
                     /locus_tag="VFH39_03165"
     CDS             complement(6267..7748)
                     /locus_tag="VFH39_03165"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HET6924802.1"
                     /translation="MQRYSNWLVRVLASRTFFIFTLCLLAFQALWLALSYKYPMLWDE
                     YYHFGLIQYYGHHLNPIIGSQPPSLDMYGNVARSPKYFYHYLMSFPFRLISLWIHNQA
                     AQVIALRVVNIALFTGGLVAFRAAMLRMTKSRALVHFILFVLIMLPLSTLLAAEVTYD
                     NMQFLLTGLVLYFALRFMQSKPLDIKWLLLTIGFGLMNSIVKYTFLPILVTVMLFLVV
                     WCWRYGWARVWHQLKDSTLQLNRWLLAALLLLVLLMGALNIERFGGNLMRYHTITPDC
                     PAVASEQRCLSFSPYKAEQAFKQQKQVWLETGSTVHIDRLPRFIVSIWVEQLYLQYFT
                     TGTQLAPDIFVVTGQLWIPFVTMLVATAIAVVCFVWKAPRLLRRADVLLVLLCAAVLA
                     LALLLVDYAAYKETSFPIDIQGRYLLPVAPFVLLVGGLGVATAIRSRKVKAVLAGLVL
                     IGLIWGGGLITHITQSQTDWYWNNRAVINTNHALHQVLWPITF"
     gene            complement(7803..8762)
                     /locus_tag="VFH39_03170"
     CDS             complement(7803..8762)
                     /locus_tag="VFH39_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6924803.1"
                     /translation="MKKYASTIITFAVLGLFVWYGLVHREIFQSLKQVSIISLVLIVI
                     GRLINIWTTGLFTKWTVEAFTDTLSQSESFVVAVLTAIGNFFGPLFGGLGIRAIYLKK
                     YHNLPYSKFTATLIGYYLMMFQFNSLLAIGSLLALPHTDQTGFVLAIFVAWFVVFFLL
                     TFVKLPPRERMRWLSRHKPGSLLTKVLYDIEDGWKVLVGNRKLLVQMLVLAVANLLTL
                     YLINYVEFRALGIHVSPAAMGLYTAIVQASLLLSITPGAVGIREAALLVLAGTLGITN
                     QQIIQVAILDRGIYFLLLGVLFMVTRHSKLRKSLVSARAAETA"
     gene            8824..9768
                     /locus_tag="VFH39_03175"
     CDS             8824..9768
                     /locus_tag="VFH39_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924804.1"
                     /translation="MQLPKTDGRRRAPLFLRLVTPQFRVGRLNKLQPAPNSVFILSTG
                     RTGTVYLADLLNQLDGVVALHEPKPSRVLNAWTTAFLEGRISVDFMTAALAGKRRKTF
                     KNVHAKRLYVESNNFIAGFADALGAVFDDPVVVHLVRDPRDFITSLTNRGDDRGIRKL
                     FNKYVPYWAYVPEGVKKRQLNALTRAAYRWVAINEYLDDFGTRSRNCHFFKFEDVFDR
                     EHPDQLHELLRAIGLTNRDISRLDFAAKSRPAQPRFSLLDRPSDSANRSKHAAMAPWR
                     EWTAAERQEVDRICGPLMRRYGYGSEPEWQVSAEGDGQ"
     gene            9815..10540
                     /locus_tag="VFH39_03180"
     CDS             9815..10540
                     /locus_tag="VFH39_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6924805.1"
                     /translation="MIKKLTKVALVIPAYNEEAVVAAVINDLHKVFEGSAYEAHVIVV
                     DDGSSDATPEVAEKAGAHVIRHILNSGSGSATATGLSYAQAHGFTVAATLDADGQHQA
                     HDVLRGVDLLVNGGADLLIGSRIIDTQASHMSLLKTIGNKGLSFITFLLFGINVTDSQ
                     SGLRIFSERALKDLKWKTSGYEFCSEMLWRAKQLGLTVAEYPIKAIYTDYSVSKPGRQ
                     NNWNGFNIVKVLLRQRIVELFSE"
     gene            10533..10895
                     /locus_tag="VFH39_03185"
     CDS             10533..10895
                     /locus_tag="VFH39_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924806.1"
                     /translation="MSRYLILMLLNLPLILAGLLGSLIDLKTGKISRGKYAFQSILWL
                     IIFAGLASAEIIYDYLHSHHLTRTPTMSLFDVIEITGIIYILFMANRSRIKADVMERR
                     LQDLHQELSIKLSEKADK"
     gene            complement(10900..>11331)
                     /locus_tag="VFH39_03190"
     CDS             complement(10900..>11331)
                     /locus_tag="VFH39_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET6924807.1"
                     /translation="AQYYSRDFITHKLLKEASIATLMPLYAGCIDQPRAEALVKMLEN
                     EHLFGPSYPVPSVPLNSPNFHPKRYWQGPTWVNTNWLIIDGLKRYGFKDHAAALTEST
                     LEMVGHGGFAEYFDPLDGEAAGGDNFSWTAALTIDLLKSKH"
BASE COUNT         2523 a         3155 c         3388 g         2265 t
ORIGIN      
        1 acttcgtcgt catccagctc gctctggctg gaaccgatat gctcggaaat catgatgccc
       61 agttcctggc gactttccag gtgcgtctct tcggcttgga agagccggtc gagcagcagc
      121 tgcaacggtt tggatacggg gtaagtgacg atgatcatca gtctgagcaa cgggctgaaa
      181 cgggcggtga aggcgagggc tcgtctggta aacaacgctt ggggaaatat ctcgccgaaa
      241 atgacgatca gcagcgtgcc gagcagaccg gcgataaccc cgaagaagac gctttccagc
      301 accagcgagg tggccgacac ggcagcgacg ttcgtcagca agatggccgc caggctgagg
      361 tgggtgttgc gccggaaacg ccggaccttg cgggcgttag aatcgccgag tttggatttg
      421 cggcgcaggt cggctgggtc gagcgccatc agcgacacgt tcagcccgga acagatggcc
      481 gacagcagca cgagcagcac tatgaccacg cagatgataa gggcgtgcat cggtcagttg
      541 accccggtcg atatggagaa gctgtgtgaa ctcacaatca ttactatttt aacgccgaca
      601 agggtggagc gcaaaccttg cagccgaggt ggctggccgc gtagagtgat aggtataagg
      661 agggtgaaat atggaagata cgatgccggt agtgactacc aatgatctgc cgggttacga
      721 ggtcgaagaa gtgtacggtg aggttttcgg cttgatcgta cggtcgcgaa atgtttttag
      781 taacttcggg gccagcttac gcaccatcgt cggcggcgaa gttaaaggct acaccgcgct
      841 gttggccgac agtcgcgaac atgccttaca gcgtctgcgg gcggccgcgg ccgaaaaggg
      901 cgccaatgcg gtactgacga tgcgcttcga cagtggcgac atcggcgggg tcatgaatga
      961 ggtcgcggcc tatggtaccg ccgtccgggt gcgcaagaaa gcctgaggcg gtggacgagc
     1021 tggcacgact gttaagcggt ccggccgaga cggtcgcccg acgactgctc ggctgcgagc
     1081 tggtccggga gctggatggg cagcggctcg tcggccgtat cgtcgaaacg gaagcgtatg
     1141 acgagacgga tccggccagc catagctatc ggggacggac taaccggacc gccgtgatgt
     1201 tcgggccgcc cggtcatctg tatgtctact tcacctacgg catgcactac tgcatgaacg
     1261 tagtgaccgg cgacgacggt cacggttcgg gggtgctgct gcgggcgctc gaaccgctgg
     1321 cgggcgaggc ggtcatgcgc cgcaatcgtc atgaccggag cgatccgagt gtcaccaacg
     1381 gaccggccaa actatgcgaa gccttggcgg tcgacagcag cctgaacggg catgatctgc
     1441 ggcaggcacc actcaagctg gtggtacggc cccctatggc aggccaggat atcgtggtgg
     1501 cgacgcgcgt cggtatcagc caggccaagg ataagccgtg gcgcttctat gtcgccgata
     1561 atcggcatgt ttcaagagta agggtgtagt gaaagttcct acgatatatt gaaaatgcga
     1621 ctccagaagc ataagcttaa tgataggaag tgtgaggctt cctgacagac ctgggtcttt
     1681 gcccgggtct ttttaattac ctcccgctat tctacgcaag ctgaatgccg agcaacccga
     1741 gcagctcggc caatttggcc ggggcctggg aatcgctacc gcctttttta acggtatccc
     1801 aggccaaatg atggagcaca tgcagtgcct cgggagtgtc caggtcgtca tccatcgccg
     1861 agaagaaatg ctccaggtac agggcagggg aaggtccgga ccgtgtcgcc agagcgcgtt
     1921 tgaataagcc aagctgttcc ttggccttgg tcagcacatc accgtgccat tcgccgccga
     1981 tgcgatagtg atattgcagc agtgccagcc gaatgacggc agggtcgtga tgtgcgagca
     2041 agtcgccggc gaagaccaga ttgccgaggc ttttgctcat cttctcaccg tagcagtaca
     2101 gcggtgcgac atgcatccag tggcggacaa aggggtacgc gctgagtgac tcgctctggg
     2161 cggtctcggc ttcgtggtgg gggaatatca gatccatgcc cccgccgtgg atgtcgatgg
     2221 gcgtgccgag caaatcggat gacatcactg agcattctat gtgccaaccg ggccgcccgg
     2281 tacccaccac actttcccag cgggccgggt catcggtatc gggcaccgct ctccaaagca
     2341 gaaaatcgaa gggatggcgc ttatcgagcc gttccggatc gccgccgcga ctggccataa
     2401 aggcggtctg caggcgacgg gtgaagccgc tctgtttgcc gaagcgcgga aaggtcgaca
     2461 catcaaaata tatgtcgccg ttggcgagcc ggtaagcgtg gccgttgtct aacagtcgct
     2521 tgacggctgc cgccatcgcc tcgatgtaat cgctggcacg cggttcggcg aaggccggca
     2581 ggaaatcgag ttgctgcagc acttcctgga aatgcgccgt ctcgtcgcgc gccaactgca
     2641 tatatggtat ccccagttcc tgcgcccggg tataaatcgg ctcatcgaca tcggttatgt
     2701 tgcgtaccag cttcacccgg tggccttggt attccaaata gcggctgagg atgtcgtagg
     2761 tgacgaacgt gaatatatgt cccatgtgag ccgcgtcata gggagttatg ccacagacgt
     2821 agatgtgcac gtcgcgggcc cacgtcgatg tttccagccg gcggctggcg gtgttgtaca
     2881 aatgcattac tatatcgagt atagcaccgg taggatgagc cctcgctgaa agccggcagc
     2941 tgaagtgcta tgctaaaacc atggcaagcg tgcaacccta tgacccgaaa atcgtcctcg
     3001 ccatcggcgc ccatgccgat gacatcgatt tcggggcttc cggcagcgtc gccgcgtggg
     3061 tggcgaaagg cgccgaagtg cattacctgg tcatcaccga tggcagcaaa ggctcagccg
     3121 acactggtat ggcgacggaa cagctggtgg cgatgcgcca acaggaacag cgcgatgccg
     3181 cggccgtgct cggcgtaaag gaagtacatt tcttgggcta cgaagacggc atgctggagg
     3241 tgacgatgga tcttaagaag gacatcgtcc ggttgatccg ccagatacgc ccggacaccg
     3301 tcgtggtgat ggatccgacc accgtctact acgccgacta caacttcata aaccaccccg
     3361 accaccgcgc ggccgggcag gcgaccctgg atgccgtctt tccgctggct cgcgaccacc
     3421 tcagcttccc ggagctgtat ttgggcgaga agctggagcc gcacaaggtc gcgcacgtgc
     3481 tgctaataaa tctggaaaag cagaattatt acgtcgacat ttccgccacg ttcgatacca
     3541 aggtccaggc attgcagcgc catgccagcc aagtaccttc ggtacaggca ttgaccgaga
     3601 tgttgcaaca acgtgccgcc gccctgggcc gcaagaccgg ttcgcagtat gccgaaggtt
     3661 tcgtgcgcat cgatacgcaa gcctaaaaca gcgttgcatg accggctatc ggtacgtcat
     3721 actgctacta atatatgtta gctatccttc ttgctgccgc taccttcgtt tcgacactgt
     3781 tgggcggcct gactgccctt aagaaccgtg accggctgca ccggttcctg ggttttgctg
     3841 ccggagtctt gctgggggtg gtggcgttcg acctcttgcc ggaaattttc agccggctac
     3901 aggagctgca tcatacgccc accggcgcga tgatcgctct ggtcggcggt ttcctgctgt
     3961 ttcatatcgt cgagaaatcg atacttatcc accactcgca agaggcggag tacgagacgc
     4021 atcaccaccc ggatgtgggc gtggccagcg ccctggcgct catcggccac agttttttgg
     4081 acggcgtcgg catcggactc ggcttcaagg ccggcaccgc cgtcggcctg acggtcgcct
     4141 tggctgtcat cgcgcatgat tttaccgacg ggctcaacac cgtcaacctg atgttgctca
     4201 atcgcaacca ctcgcggcag gccgttacgt tcctggtgct cgatgcgttg gcgcctatcg
     4261 tcggtgtgct cagtacgctg tggttcacta tttcgtccaa cttgctggtg ctgtacttag
     4321 gtttttttgc cggcttcttg ttgtatatcg gtgccagcga aatcctgccg gaggctcaca
     4381 gcaagcattc gtcgtacgcc acaatcgcac tcaccatctt gggggcggtt tttatcttcg
     4441 tggtgacgcg ggtcgccatc tgattaagca gcgcagtggt aaaatagaag tatgttagct
     4501 gcggttatcg tcctgggggt tttggtggta gcgctgctgc tggtcgtgtt ccgccagcag
     4561 ctcacgcccc ggcacgatgg caacagcgca ctgcttataa aagaagatct gaagacgctc
     4621 agcgaagaca tcaccaagct gaaggacggc ctgcagcaac agctgagcga tcagctcggt
     4681 gccagcagca agcagatgtc cgcccagttc caagctagcg ccaagatcat ccgtgacgtc
     4741 accgaaaagc tcaccgagct ggaccggacg aacaagaatg tcggcgacat cgcatcggag
     4801 ctgaagctgc tacagaatgt cttgcaaaac cccaagcaac gcggtgtctt aggcgagtac
     4861 tacctggagc agatactgaa gaatctgctg ccacccggca gctacgagct gcagtacaag
     4921 ctgggcgaag gattgattgt cgatgccgtc atcaagctgg atgacaaagt gctgcccatc
     4981 gacagcaagt tctcgttaga gaactacaac cgtctgctgg aggccgccga cggcgaccgc
     5041 gacatactgg aacgggcttt taaacaagac ctgaagcagc gtatcgaaga aacctccaaa
     5101 tatatcaagc cgggcaaggg gacgctcgac caggcgctga tgttcatccc gagtgaggcg
     5161 atatattatg acctgctggc caacaaggtg ggcttaggca acgtgtccgg gcgcaacctc
     5221 atgcaatacg cggcggccga caagcacgtg accatcgtgg gtccgagcac cctcagcgcc
     5281 atgctgcaga ctatcgtgca ggggctgcgc tcgctggaga tccacaagga tactgagaag
     5341 atccgtcaga acatcgaaca gctcggtaaa cacctcatcg cccataacgc ctacatgcag
     5401 aagttaggta acagcctcgg tacgaccgtc ggtcatttca acaccacata caaagaattg
     5461 ggtaagatcg acaaagatat cgtcaaaatc acttccagcg agccgcacgt ggaaccgatg
     5521 gcccttgagc ggccatcgct agacgactag ctgcaaaaag caataaatgt tataatactc
     5581 ttaaccaaag gagtattgtc atggctaaag ccgagctggc gcccccgtcc aatcgtttat
     5641 ttatcccggg tagtgaggcc gtggaagtcg gtggcgtacg acgtatcgga ccgaattctg
     5701 tacggcgggt tgagcgggcc gcgcaccact acatgaaata tcgcgatcag ttcaagcgcc
     5761 gaggtgcgtt tatcttatgc agcggcggtt actccggttc agcccacggg caagaggcgc
     5821 cggtggatag gtcgcagcgg gaagcccgct tgctaagcaa ctgcgagatg agtacgacat
     5881 tccctcacac ttgattgaag aagaggacga atcgacgagc gccttcagta acgtggccaa
     5941 ttcgatcttg ggcggctggg tagagccgac ggcgttcaat gccgaggatc ggctcggtgt
     6001 ggtagcgccg cccctcttcc agaaacgcct gcaaatcata tggagtaaag ttaacattgg
     6061 ccgtgaggcg atcgaaccaa tatgcgacaa cgagaacatg tcgtttgcca ctgagctcgc
     6121 cggcaagctg atcacccgtt atctgctccg aagtgcaacc gagccggccg atttgctgca
     6181 ggccgaagcc cggttcaatc agctgccgat tgccggtcgt tccggcaaca ctgcctgaac
     6241 cggcagcagc gtaaatcggg ctcaagctag aaggtaatcg gccagagcac ctgatgcaaa
     6301 gcgtggttgg tgtttatgac cgcccggttg ttccaatacc aatcggtctg gctctgggtt
     6361 atatgcgtga tgaggccacc gccccatatg agtccgatca gtaccagtcc ggctagcact
     6421 gccttgacct ttcgcgaacg gatggcggtg gctaccccaa ggccaccgac cagcagcacg
     6481 aaaggcgcca ccggaagcag gtaccgtccc tggatgtcga tggggaagct ggtctcttta
     6541 taggcggcgt agtcaaccaa cagcagggcc agggcaagca cggccgcaca cagcagcacc
     6601 agcagtacat cggcacgtcg cagtaaccgt ggtgctttcc atacgaaaca cacgacggcg
     6661 atggcggtcg cgaccagcat ggtgacgaac ggaatccaca gttggccggt caccacgaag
     6721 atatcgggag ccagttgggt gccggtcgtg aagtactgca ggtagagctg ctccacccag
     6781 atggatacga tgaatcgcgg cagcctgtcg atatggacgg tcgaaccggt ttcaagccac
     6841 acttgtttct gctgtttgaa ggcctgctcg gccttgtagg gcgaaaagct cagacaacgc
     6901 tgctcgcttg cgactgccgg gcaatccggc gtgatggtat ggtaacgcat cagattgccg
     6961 ccgaatcgct cgatattcag cgcccccatc agtagcacca gcagcaaaag agcagccagt
     7021 agccagcggt tcagctgcag tgtcgaatcc ttaagctggt gccagacccg tgcccatccg
     7081 tatcgccagc accacacaac caaaaacaac atcaccgtca ccaatatggg caggaaagtg
     7141 tacttgacga tgctgttcat aagaccgaag ccgatggtca gcaacaacca cttaatgtcg
     7201 aggggcttgc tttgcatgaa ccgcaaggcg aaatacagta ccaggccggt cagcaggaac
     7261 tgcatattgt cgtaggtgac ctcagcggcc aggagcgtag ataatggcag catgatgagc
     7321 acgaacaaaa tgaagtgcac tagggcacgt gactttgtca tccgtagcat agccgcccgg
     7381 aaggcgacca ggccgcccgt gaacagggcg atgttcacta cacgcagcgc gataacctgc
     7441 gccgcctgat tgtgaatcca caaacttatg agccggaacg ggaagctcat gaggtagtga
     7501 tagaagtact tcggcgaacg cgcgacgtta ccgtacatat caaggctggg tggctggctg
     7561 ccgatgatcg ggttcaggtg gtgcccgtag tactgtataa gcccgaagtg gtagtactca
     7621 tcccacagca tggggtattt gtacgacagc gccagccaga gcgcctgaaa cgccaggagg
     7681 cagagggtga aaataaaaaa tgtccggctc gccagtacgc gcaccagcca gtttgagtat
     7741 cgctgcatca gcttccagta tacacggccg gttgaggcta acctgagcac tcaagcgaca
     7801 cgttaggcag tttcggcggc tcgggcgctg acgagcgatt tgcgcagctt ggaatgccgg
     7861 gtgaccatga acagcacgcc tagcagcaag aagtaaatac cgcggtcgag gatggcgacc
     7921 tgtatgatct gctgattggt aatgccgagc gtaccggcca gcaccagcag ggcggcttcc
     7981 cggatgccga cggcaccggg ggtaatgctg agcagcagcg atgcctgtac gatggcggtg
     8041 tacagcccca tggccgccgg gctgacatgg atgccgagcg cccggaattc gacgtagttt
     8101 atgaggtaca gcgtcagcag gttggccacc gccagcacga gcatctggac gagcagttta
     8161 cggttgccga ctagcacctt ccagccatcc tcgatgtcat acagcacttt ggtgagcaag
     8221 gagcccggct tatgtcggct taaccacctc atacgttcgc gcggcggcag cttgacgaac
     8281 gtgaggagga agaacaccac gaaccaggcg acaaagatag ccagcacgaa gccggtctgg
     8341 tcggtatgcg gcagcgccag cagcgaaccg atggccagta acgagttgaa ctggaacatc
     8401 atcaggtagt agccgatgag cgtggcggtg aatttgctgt agggtaggtt gtggtacttc
     8461 ttcaggtaaa tggcccggat gccgaggccg ccgaacaacg gaccgaagaa gttaccgatg
     8521 gctgtcagga cggcgactac gaagctttcg ctctggctta aagtgtcggt aaaggcttcg
     8581 acggtccatt tggtaaacag accggtggtc cagatgttga tgagccggcc gatgacgatg
     8641 agtaccaggc tgatgatcga cacctgcttc aggctctgga atatctcccg gtgaaccagg
     8701 ccgtaccaca cgaacaatcc gagtacggca aaggtgatga tggttgaggc gtatttcttc
     8761 ataaataggc ggtgtacgcg cagcggggta ctgctccacc gtacgatttg ctagtattgt
     8821 agcatgcaac ttcccaagac agacggccgc cgccgggcgc cgttattttt acgattggta
     8881 accccgcagt tccgagtagg ccgcctcaat aagttacagc cggcgccgaa cagcgtgttc
     8941 atcttgtcga ccggccgcac cggcacggtc tatctggcag acttgttgaa tcagctggac
     9001 ggcgtggtag ccttgcacga gccgaagccg tcgcgggtgc tcaatgcctg gacgaccgct
     9061 tttttggaag gccggatatc cgtcgatttc atgacggccg ccttggccgg taaacgccgc
     9121 aaaactttca aaaatgtgca cgccaagcgg ttgtacgtcg agtcgaacaa cttcatcgct
     9181 ggcttcgccg atgcgttggg ggcggtcttt gatgatccgg ttgtcgtgca cctcgtccgc
     9241 gacccgcggg acttcataac gtcgcttacc aatcgcggcg acgaccgcgg tatccgcaag
     9301 ctgttcaata agtacgtacc ctattgggca tatgtaccag aaggggtaaa aaagcggcaa
     9361 ctgaacgccc tgaccagggc tgcctaccgc tgggtagcta tcaacgaata tctcgatgac
     9421 ttcggtacac gctcccgcaa ctgccatttt ttcaaattcg aagatgtttt tgatcgtgag
     9481 catccggatc aactgcacga actgctgcgg gccatcggac tgacgaatcg tgatatcagc
     9541 cgtctcgact tcgccgccaa aagccggccg gcgcagccgc gtttcagtct gcttgatcgg
     9601 ccgagcgatt ccgccaaccg ttcgaagcac gcggccatgg caccctggcg ggaatggacg
     9661 gccgccgagc ggcaggaggt cgatcgcatc tgcggaccgc tcatgcgccg gtacggctac
     9721 ggctccgaac cggaatggca ggtctctgca gagggagacg gccagtgaca cagcctcagc
     9781 aactcttaag cgattttgct ataatgcaat agacatgatt aaaaagctaa cgaaggtcgc
     9841 gctggtgatt ccggcttata acgaagaagc cgtggtggcg gctgtgatca acgatcttca
     9901 taaagtattc gaaggcagtg cctacgaggc gcacgtcatc gtcgtcgacg acggttccag
     9961 cgatgccacc ccggaagtcg ccgaaaaggc cggggcgcat gtcatccgcc acatccttaa
    10021 ttccggcagc ggcagcgcga ccgctaccgg gttgagctat gcgcaggccc atggatttac
    10081 cgtcgcggcc accctcgatg ccgacggcca gcaccaggcc catgacgtgc tgcggggcgt
    10141 cgacctgctg gtgaacggtg gcgccgactt gctgatcggc agccggatca tcgacacgca
    10201 ggccagccat atgtcattgc ttaagactat tggcaacaaa ggtctcagct ttatcacttt
    10261 tttgctgttc ggcatcaatg tcaccgactc gcaatcgggt ttgcgcatat tttcggagcg
    10321 agccctgaag gatctgaagt ggaagaccag cggctacgag ttctgttccg aaatgttgtg
    10381 gcgcgccaag cagctggggc tgacggtagc ggagtacccc atcaaggcca tttacaccga
    10441 ttactccgtt tccaaaccgg gtcgccagaa caactggaac ggcttcaata tcgtgaaggt
    10501 actgctaagg cagcggatcg tggagctgtt cagtgagtag gtatctcatc cttatgctgt
    10561 tgaacctgcc acttatactg gccggcctgc tcggcagcct catcgatctc aagaccggca
    10621 agatctcccg cggcaaatat gcgttccaaa gcatattgtg gctgattatc ttcgcgggcc
    10681 tggcgtcggc cgaaatcatt tacgattacc tgcactcgca ccacctgacc cgtaccccca
    10741 cgatgagcct gttcgacgtc atagaaatca ccggcatcat ctacattttg ttcatggcca
    10801 accgctcgcg catcaaggcc gacgtcatgg agcgccgttt gcaggacctg caccaggagc
    10861 tgtcgatcaa actgtccgaa aaagccgaca aatagcaggc tagtgttttg atttgagcag
    10921 gtcgatcgtc agcgcggcgg tccagctgaa gttgtccccg ccggctgctt caccgtccag
    10981 agggtcgaaa tattcggcga aaccgccgtg gccgaccatc tcgagtgtgc tctcggtgag
    11041 cgcggcggca tgatccttga agccgtaacg cttgagcccg tcgataatca gccagttggt
    11101 gttcacccag gtcggccctt gccagtagcg cttggggtgg aaattgggcg aattcaacgg
    11161 cacgctcggc acggggtagg atggcccgaa cagatgctcg ttctcgagca tcttcaccaa
    11221 tgcttcggcc cgcggctggt cgatgcagcc ggcgtacagc ggcatgaggg tggcgatgga
    11281 cgcctctttc agcagtttgt gggtgatgaa atcgcgcgaa taatactgtg c
//
LOCUS       DASSFD010000135         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1428564, whole genome shotgun sequence.
ACCESSION   DASSFD010000135 DASSFD010000000
VERSION     DASSFD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428564"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="pgk"
                     /locus_tag="VFH39_03195"
     CDS             <1..532
                     /gene="pgk"
                     /locus_tag="VFH39_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6924808.1"
                     /translation="HKAEEKAKDGRFVFALPHDGVVAHKVDRTAPTRIVDWGTDVVAE
                     IESYPRRPAPVSRHVQPHEMILDIGPFSASFIAGGIQLAGTVVWNGAVGVTETEGLHD
                     PIGPTAHGTETIVEAMLGKFGHRPFSLVGGGDTVGFVESRKLVSSFDHVSTGGGASLE
                     LMAGRKLPGVEALEDK"
     gene            579..1361
                     /gene="tpiA"
                     /locus_tag="VFH39_03200"
     CDS             579..1361
                     /gene="tpiA"
                     /locus_tag="VFH39_03200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET6924809.1"
                     /translation="MLVVGNWKMHLNTHEASTLVHRLGQRIKTHRDVEVVLAPSMLCL
                     QPLSLEIDRRKFRLSAQDAYYKDDGPYTGEVSFAMVRDLVHYGIVGHSARRIYFNESL
                     ETIRDKVQAAVRNDIVPILCVGETKQERDQGETKQVIHDQLTTALSNLTSEDILQTVI
                     AYEPVWAISTFDGETSKPDDMAKAFSYIRFQIAELYGKHASEQVRVLYGGSVNDQDAR
                     AYLELEGCDGVLVGAASLSAHQFASIVETAAAVATERSKHHG"
     gene            1354..2250
                     /locus_tag="VFH39_03205"
     CDS             1354..2250
                     /locus_tag="VFH39_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924810.1"
                     /translation="MANQKSSKVFDVAKPGKSAPSSSAKPIIITNRPVLKDPMVTDDD
                     TSADEERPAETRTTTKTRVQIKPIHDLTAETADDQPETAAPETPATTEAEAPSAPDLP
                     AAKASKPKPPKPVAEKAEAKTGAQPRIVTGTTDAEPKATAKPETSAESAVEAKEPATA
                     PAAPAPESESEAPDAAATAPAKEPEATEEPKDTAAAAAPAESPAESDPSGDVVAATGD
                     NAAELAAKREQERAEELEKLALSRKYYLPINQTGRRHARHMAWLGVFVIILLALAWAD
                     VALDAGLIKIPGVKAPVRIINK"
     gene            2341..>3503
                     /locus_tag="VFH39_03210"
     CDS             2341..>3503
                     /locus_tag="VFH39_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6924811.1"
                     /translation="METDLLVGLNPEQRRAVETTDGPLLIQAGAGSGKTKTLTHRIAY
                     LIATHRATPYNILAVTFTNKAAREMRERVAQLLGERSDNRSFMPFMGTFHSICVRLLR
                     QDGEHIGIARSFVIFDEADRLTAVKQASKTAGIDEKSFPPRVLSSLISSAKNDMMTAD
                     ELASQGAPAQRAAAQVMPLYQAALREASALDFDDLINKTVELLQTKREVRERWQQQFR
                     YVMIDEYQDTNAAQYKLVNLLTNSDHNIAVVGDDWQSIYSWRGADFRNILNFERDYKN
                     CTVIKLEQNYRSTGNILRAAHGVITRNQQRSDKELWTEAGDGLPVQLLQVNGERAEGE
                     AIVRRVRAAVDSGVRRFRDFAVLYRTNAQSRSVEETFLRYGIPYRVVGGVRFY"
BASE COUNT          805 a         1082 c         1038 g          578 t
ORIGIN      
        1 gcataaagcc gaggaaaagg cgaaagacgg acggttcgtg ttcgccctgc cccacgatgg
       61 cgtcgttgca cacaaagttg accggaccgc tccgactcgc atcgtcgatt ggggcaccga
      121 tgtggtggcg gagatcgaaa gctacccacg ccgcccggcg ccggtcagcc ggcatgtgca
      181 accgcacgag atgattctgg acatcggtcc gttcagcgcc agcttcatag ccggcggtat
      241 ccagctggcc gggaccgtag tgtggaacgg tgcggtcggc gtgaccgaga ccgaagggct
      301 acatgaccct atcggcccca cggcccacgg taccgaaacc atcgttgaag ccatgctcgg
      361 caagttcggt caccggccct tcagcctggt gggcggcggc gacacggtcg gcttcgtgga
      421 aagtcgtaag ctggtcagca gtttcgatca cgtctcgacc ggcggcggcg ccagcctgga
      481 gctgatggcc ggccgcaagc tgccgggggt ggaagcgctg gaggacaaat gatggtacac
      541 ttgaatcaaa ctaagcgtaa gcgaaaacaa tgaaacgaat gctcgtcgtc ggaaactgga
      601 agatgcacct caacacgcat gaggcgtcaa ccctggtgca ccgtctgggc cagcgcatta
      661 aaacccaccg cgatgtcgaa gtcgtgctgg caccaagcat gctctgtctg cagccactca
      721 gcctcgagat cgaccgccgc aagttccgct tgagcgccca ggacgcatat tacaaagatg
      781 acgggccata caccggcgaa gtttctttcg ctatggtgcg cgacctggtc cactacggca
      841 tcgtcgggca ctcagcccgc cggatttatt tcaatgagag cctcgaaacc atccgtgata
      901 aggtccaggc agccgtccgc aacgacatcg tgcctatctt gtgcgtgggc gagaccaaac
      961 aggagcgcga ccagggcgag accaaacagg tcatccacga tcagctgacg accgccctta
     1021 gcaatctgac ctccgaagac atattgcaga cggtcatcgc ctatgagccg gtctgggcca
     1081 tcagtacatt cgacggtgaa acgtcgaagc cggacgatat ggctaaggct ttcagttaca
     1141 tccgtttcca aatcgccgag ctttatggca agcatgccag cgagcaggta cgagtgttgt
     1201 atggcggcag cgtgaacgac caggatgccc gggcttatct tgaactggaa ggctgcgacg
     1261 gcgtattggt cggggcggcc agtctgagcg cccatcagtt cgcttccatc gtcgaaacgg
     1321 cggccgccgt cgccacggaa cggtccaagc accatggcta atcagaagtc gtccaaagtt
     1381 ttcgacgtcg ccaaaccggg caaatcggcc ccgtccagta gtgcgaagcc gatcatcata
     1441 accaatcgcc cagttctcaa agatccgatg gtcaccgacg acgatacctc agccgatgag
     1501 gaaagaccgg ccgaaacgcg caccaccacc aaaacgcggg tccaaataaa gcccatccat
     1561 gatctgaccg ccgagaccgc cgatgaccaa cctgagacgg ctgcgccgga gactcccgca
     1621 actaccgaag cggaagcacc atcagcgccc gacctgccag ctgccaaggc gtccaaacct
     1681 aaacccccca agccggtggc cgaaaaagcc gaagctaaga ccggggccca accacgtata
     1741 gtgaccggta ccaccgatgc cgaaccgaag gctacggcca agccggaaac ctctgctgaa
     1801 tcggccgtcg aagccaaaga gccggcaacg gcacccgccg cacctgcgcc ggagtcggaa
     1861 tccgaagcgc cagatgcggc agccactgcg ccagccaagg agccggaagc aaccgaagaa
     1921 ccaaaagaca ccgccgctgc ggccgcccca gccgaaagtc cggcagaatc cgacccgagc
     1981 ggggacgtcg tggcggcaac cggtgataat gctgccgagc tggctgccaa gcgtgagcag
     2041 gaacgggccg aggaactcga gaagctggcc cttagccgca aatattactt gccgatcaac
     2101 cagacgggcc gccgccatgc ccggcacatg gcctggctcg gagtatttgt cattattttg
     2161 ctggcgctgg cctgggcaga tgtagccctc gacgccggcc ttataaagat tccaggcgtc
     2221 aaagccccgg tgcgtatcat caacaaatag ctgactggtg ctgcatgccg gtcgtatatt
     2281 gctgagtatg tatattcggc aacgtattgg ccgggtggta attgttatac ttaaacgtgc
     2341 atggaaacag atttactcgt ggggctgaat ccggaacagc gccgggcggt cgagaccacc
     2401 gacggcccgc tgctgattca agccggcgcc ggttccggca agaccaagac gctcacgcat
     2461 cgcatcgcgt acctcatcgc cacgcaccgg gcaacacctt ataacatcct ggccgtcacc
     2521 tttacgaata aggcggcgcg cgagatgcgc gaacgggtgg cccagctgct gggcgagcgg
     2581 tccgacaacc gctccttcat gccgttcatg ggcacgttcc acagcatctg cgtgcggctg
     2641 ttgcggcagg acggcgaaca tatcggtatt gcccggagct tcgtcatctt cgacgaggcc
     2701 gaccggctga cggccgtcaa acaggccagc aaaaccgctg gcatcgacga gaagagcttt
     2761 ccgccacgcg tattaagcag cctcatcagc agtgccaaaa acgatatgat gacggccgat
     2821 gaactggcgt cgcagggcgc accggcgcag cgagctgcgg cgcaggtgat gcccctgtac
     2881 caggcggcgc tccgcgaagc ctcggcgctc gatttcgacg accttataaa caaaacggtc
     2941 gaactgctgc agaccaaacg cgaggtacgc gagcgctggc aacagcagtt ccgctacgtc
     3001 atgatcgacg agtatcagga caccaacgcc gcccagtaca aattagttaa cttgttaact
     3061 aatagcgacc ataatatcgc ggtggtgggc gatgactggc agagcattta cagctggcgc
     3121 ggtgctgact tccgcaatat cctcaacttc gaacgcgatt ataagaactg taccgtcatc
     3181 aagctggaac agaactatcg cagcaccggc aacattttgc gcgccgcgca cggtgtcatc
     3241 acgcgcaacc agcagcgcag cgacaaggaa ctgtggacgg aggccggtga cggcctgccg
     3301 gtccagctgc tgcaagtcaa cggtgaacgg gcggaaggcg aagccatcgt ccggcgggtc
     3361 cgcgcggccg tcgacagcgg cgtacggcgc ttccgggatt tcgcggtgct gtaccgtacc
     3421 aacgcccaga gccgttcggt cgaagaaacg ttcctgcgct acggtatccc gtaccgggtg
     3481 gtcggcggcg tgcgcttcta cga
//
LOCUS       DASSFD010000136         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1192744, whole genome shotgun sequence.
ACCESSION   DASSFD010000136 DASSFD010000000
VERSION     DASSFD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192744"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..462)
                     /locus_tag="VFH39_03215"
     CDS             complement(46..462)
                     /locus_tag="VFH39_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6924812.1"
                     /translation="MDTAPQDLHKLLQHIATIHARESDQILLEQFGIGYSQYKILGSI
                     KNNPALRQKHIAFELGQTEASVSRQINLLQERGMLTVRVNPTNRREHSVVITRKAERL
                     MEAAEQVLANYQSASLSHLSKQQQNQLLNLLATIFK"
     gene            complement(493..1272)
                     /gene="rsmA"
                     /locus_tag="VFH39_03220"
     CDS             complement(493..1272)
                     /gene="rsmA"
                     /locus_tag="VFH39_03220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET6924813.1"
                     /translation="MSEPIPKKSLGQHWLMDETALAAIADAAELTPDDTVLEVGPGLG
                     PLTQLLTERAGQVVAVEFDEALAAELPRRVPTPNLKVIRQDILSFDFTSLPPDYKVVA
                     NIPYYLTSNFIRVLSETTNRPSVAVLLVQQEVAERVTASPGDMSVLSVTAQYYWRVGL
                     GFRVPAELFTPPPKVDSQVLLLERRDEELFPGTDTKQFFRLVKAGFSERRKKLRSSLS
                     NGMHVSKPDAEAILNKASIDPNLRAQALSLDDWHRLYVANT"
     gene            complement(1315..2577)
                     /locus_tag="VFH39_03225"
     CDS             complement(1315..2577)
                     /locus_tag="VFH39_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET6924814.1"
                     /translation="MHRIKKLIPLSDDLWQPPKRYRRLRSHPVMIPVVTFIGLLIVSI
                     IALVLLRTHDAPVANAYVVIVSYDHHEQTVPSREPTVGALLQKLHIRLNEGDRVEPSL
                     PTKINQEDFRINVYRAVPVEIVDGNNKTFTFSAATTPRSIARQAGIITYAEDDLSNQP
                     VTNFVAARSIGEQVVIDRSTPVNINLYGALTVTHTHADTVGQLIREKHIRLAKNDQVT
                     PSFATPLTPGTQVVIARNGTKLETVKEDIPMPVQTIQDDSLAFGTSAIRQQGSAGQKV
                     TTYQDKLVNGQVVGRTPIQSVITQAAVTQIVVVGTSLSGIKGDMALAGIGAGDYNYVD
                     YIVSHESGWCPTKAQGEHYCPATPDNQYTSAGYGLCQATPGSKMSASGDDWATNPVTQ
                     LRWCNGYAVGHYGSWGAAYNHWISYHWW"
     gene            complement(2680..>3262)
                     /locus_tag="VFH39_03230"
     CDS             complement(2680..>3262)
                     /locus_tag="VFH39_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6924815.1"
                     /translation="YQDLGLDGFLEEVSLVSDLDSADLSGDAVTLMTLHAAKGLEFPV
                     VFMTGLEETIFPHSRALYDPSEMEEERRLCYVGMTRAREELYMIHATSRMLYGGLQHN
                     PPSRFLAEVDGDFQSADTYDQPPQLLGTDPFKPADDGEPRYVPELNEGDGVRHSTFGV
                     GTVLELSGDTAVIYFKGKGTRKLNIAIAPLEKL"
BASE COUNT          599 a          934 c          996 g          733 t
ORIGIN      
        1 ccacaaatat aagcattatg gccatagcta gcggtggtcg catgactact tgaaaatggt
       61 agcgagaaga ttgagtagct ggttctgctg ctgcttgctc aggtggctga gcgaggccga
      121 ctggtagttg gccagcacct gctcggccgc ttccatgagg cgttcggcct tacgggtgat
      181 gaccaccgag tgctcacggc ggttggtcgg gttgacccgc acggtgagca tgccgcgttc
      241 ctgcagcagg tttatctgcc ggctgacgct ggcctcagtc tggcccagtt cgaaggcgat
      301 gtgcttctga cgcagggcag gattgttctt gatgctgccg aggattttgt actggctgta
      361 gccgatgccg aactgttcga ggagaatctg gtcgctctcg cgggcgtgga tggtcgcgat
      421 gtgctgcaac agcttgtgca gatcctgcgg agcggtgtcc atagcttcat agtagccgct
      481 tcgcttagct tgtcaagtat ttgctacgta cagtcggtgc cagtcatcga gtgacaacgc
      541 ctgggcccgc aggtttggat cgatacttgc tttgttaagt atggcttcgg catccggttt
      601 actcacatgc ataccgttgc taaggctgct gcgcagcttt ttgcggcgtt cactgaatcc
      661 ggcctttacc aggcggaaaa actgcttcgt atctgttcct ggaaacagct cttcatcgcg
      721 tcgctccaat aacagcacct gactgtcgac cttcggcggc ggcgtgaaca gctcggccgg
      781 tactcggaag ccgagcccga cccgccagta gtattgtgcg gtcacgctca gcaccgacat
      841 gtcgcccggt gaggcggtga cccgctcggc gacctcctgc tgcactaaca gcacggcgac
      901 gctcgggcgg ttggtggttt cgctcaaaac acggatgaag ttgctcgtca ggtagtaggg
      961 gatgttcgcc actactttgt agtccggagg caggctcgtg aagtcgaaac ttaagatgtc
     1021 ctgccgaata accttgaggt tgggcgttgg cacacgccgc ggcagctcgg cggccagcgc
     1081 ttcatcgaac tcgacggcca ccacttgccc ggcccgctcc gtaagcagtt gcgtaagcgg
     1141 cccgagaccc gggcctacct caagcacggt atcgtctggt gtcagctcgg ccgcatcggc
     1201 gatggctgcc agggcggtct cgtccatcag ccagtgctgc cccagcgact tcttaggaat
     1261 gggttcactc atccgttcaa tagtagcagt cgctctgccg atttactacc gatgctacca
     1321 ccagtggtag gaaatccagt gattgtaagc cgctccccat gaaccgtagt gacccaccgc
     1381 ataaccgttg caccagcgca gctgcgtcac cgggttggtc gcccagtcgt cgccggaagc
     1441 ggacatcttg ctgcccggcg tcgcctggca taagccgtaa ccggccgagg tgtactgatt
     1501 atccggggtg gccggacagt aatgctcgcc ctgcgctttg gtgggacacc accctgactc
     1561 gtggctgacg atgtaatcga cgtagttgta atcaccggca ccgatgcctg ccagggccat
     1621 gtcccccttg ataccactga gcgaagtacc gaccacgaca atctgcgtca cggcggcctg
     1681 cgtgattacc gactgaatcg gtgtccgccc caccacctgg ccgttgacca gcttgtcctg
     1741 gtaagtcgta actttctggc cggccgaacc ctgctgccgt atggcgctgg taccgaatgc
     1801 caggctgtcg tcctggatgg tctgcacggg catgggtatg tcttctttta ctgtttccag
     1861 cttggtgccg ttacgggcga taaccacttg agtacctggg gtcagaggcg tcgcgaagga
     1921 cggcgttacc tgatcgtttt tagccaggcg gatatgtttt tcacggatga gctgcccgac
     1981 ggtatcggca tgggtgtgcg tgaccgtcag ggcgccgtac aggtttatat tgacgggcgt
     2041 cgaacggtca atgacgacct gctcgcctat agaacgggca gctacgaaat tggtcaccgg
     2101 ctggttggac agatcatcct cggcataggt gataataccg gcctgccggg caatactgcg
     2161 cggcgtagtg gcagcgctga aggtaaaggt cttattattg ccgtcgacaa tctcgaccgg
     2221 tacggctcgg tacacgttaa tgcgaaaatc ttcctggttg atcttcgtgg gcaggcttgg
     2281 ttcgacccgg tcgccttcgt tgagccggat gtgcagcttc tgcagtagcg cgccaacggt
     2341 cggctcgcgg ctgggtaccg tctgttcgtg atggtcataa gaaacgatga ccacataggc
     2401 gttcgctacc ggcgcgtcgt gcgtcctaag cagcaccagc gcgatgatgc tgactatcaa
     2461 caagccgatg aaggtgacga ccggtatcat gaccgggtgt gaccgtagtc tgcggtaccg
     2521 cttgggcggc tgccacaggt catcgctcag tggaatgagt tttttgattc tatgcattac
     2581 agggggagtg cggtgacgtc gttaccctgt tacgccacgc accgaagcca tgctaaatag
     2641 gcgctttcat tataccaaac ggtctgccgc acgctgccgt tacagttttt cgagcggggc
     2701 gatggcgatg ttgagcttgc gggtgccctt gcccttgaag tagatgaccg ccgtatcgcc
     2761 gctgagctcc aggaccgtgc caaccccgaa cgtgctgtgc cgtacgccgt cgccttcgtt
     2821 cagctcgggc acataacgcg gttcgccatc atcggccggc ttgaacgggt cggtgcccaa
     2881 cagctgcggg ggctggtcgt acgtgtcggc cgactggaag tcgccgtcta cctcagccaa
     2941 aaagcgcgac ggcgggttgt gctgcaggcc cccgtacagc atgcgcgagg tggcatggat
     3001 catgtacagc tcttcgcggg cacgggtcat gccgacgtag cacagccggc gctcctcttc
     3061 catctcggaa ggatcgtaca gcgcgcggga gtgcggaaaa atggtttctt cgagtcccgt
     3121 catgaatacc accgggaatt ctaggccttt ggcggcgtgc agggtcatga gcgtgacagc
     3181 atcgccgctc aggtcggcgc tatccagatc gcttaccaga cttacctcct ccaggaagcc
     3241 atcaaggccg aggtcctggt at
//
LOCUS       DASSFD010000137         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1701052, whole genome shotgun sequence.
ACCESSION   DASSFD010000137 DASSFD010000000
VERSION     DASSFD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701052"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFH39_03235"
     CDS             <1..811
                     /locus_tag="VFH39_03235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6924816.1"
                     /translation="GSADTTRKLLQEEKDVKLVALPKNIGAAARNEGVIRADTPYIAF
                     CDDDTYWEADGLRIAADMLDKYPALGLVNARIVVGDEEKPDPISEQMEDSPLSDEAGI
                     PGKVLMSFMGGASMVRKDAWVEVGGYDRKMFIGGEEETVGWKLAKAGWEMRYVPEVVV
                     HHYPTMQNAAGLQDYGIRNTIWNAWLHRPAKRAWGWTWHVISSSPKNRILLKGILKTL
                     PGIPWVARNRDVMKPNVEAKVRRLELQRLEGDNARQYGRDLKRSGTQGASS"
     gene            complement(814..1761)
                     /locus_tag="VFH39_03240"
     CDS             complement(814..1761)
                     /locus_tag="VFH39_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924817.1"
                     /translation="MQRSNYPTPEAMCAYPTEHWLTADVDEMRRHDFDTVLFCVTEDM
                     LRRRDMSVRPVVEHTKQQGLSCEADLWGFAKVLGGEPPSAFAADPSQQACLNNPDFRS
                     LMSDAIDYVQALGFDRLFIDEPNFANVENPNPCSCCQGKERELVRLMADYAGRLSIRS
                     TVCMISTEHGYKVLESLAAERTVHGVATDPYYSNFPSSHDSPHRYVGGWARRLKGLEA
                     RYGVKPQIAVQGFHLPMPMDDKRAWRAEADARPWHEIPLWAAEAALAEGVRRILFWAY
                     QACSDFPELAPPYPERVWDMTRTINARVHHADPAGHAGP"
     gene            complement(1858..>2431)
                     /locus_tag="VFH39_03245"
     CDS             complement(1858..>2431)
                     /locus_tag="VFH39_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET6924818.1"
                     /translation="LSDFFDAMGTIVGVSNEAGLLDKKGRLPVINRALFVDGFAAIAG
                     GLGSSSSNTAFIESASGVGEGARTGVASIVTGGLFVVALFLTPLTTIVPSQAATPALV
                     IVGFLLATQVRNIPWEDYEVAIPAFLAIVLMPFTYSITNGIGAAVIAYVLLRIVNGRF
                     RKTHWLLSVIALLFVVYFFLSPIEHLLNVA"
BASE COUNT          534 a          687 c          763 g          447 t
ORIGIN      
        1 cgggtcggct gacaccacgc ggaagctgtt gcaagaagag aaggacgtca aactggtggc
       61 gctgcccaag aatatcggcg ctgccgcccg gaacgaaggc gttattcgag ccgacacgcc
      121 atatatcgcc ttctgcgacg acgatacata ctgggaagcg gacggtttac ggatcgcggc
      181 cgacatgctg gacaaatacc cggctctagg gttggtgaac gcccgcatcg tcgtcggtga
      241 cgaggagaag ccggatccaa tttcggagca gatggaagac agcccgttgt ccgatgaagc
      301 cggcataccc ggcaaggtac tcatgagttt catgggcggc gcgtccatgg tgcgtaagga
      361 cgcctgggtg gaagtgggcg gctatgaccg caagatgttc atcggtggtg aagaagagac
      421 tgtggggtgg aagctcgcca aggccggttg ggaaatgcgt tacgtcccag aggtcgtggt
      481 gcatcactac ccgacgatgc agaacgccgc cggtctgcag gactatggca tccgcaatac
      541 catctggaac gcctggttgc atcggcctgc caagcgggct tggggttgga cgtggcacgt
      601 cataagcagc tctccaaaga accgcatcct gctgaaaggc atcctcaaaa ccttacccgg
      661 aataccgtgg gtggcccgta accgcgatgt catgaaaccg aacgtagaag ccaaggtacg
      721 acggcttgaa ttgcagcgcc tggaagggga taacgcccgt cagtacggcc gcgatctgaa
      781 gcgttccggc acgcagggcg cttcgtccta gccttagggt ccggcatggc cagccgggtc
      841 agcgtggtgg acgcgcgcat ttatggtgcg ggtcatgtcc cacacgcgtt cagggtaggg
      901 cggcgccagc tccggaaagt cactgcacgc ctgataagcc cagaataaaa tgcgacgaac
      961 tccttcggcc aaagccgctt cggcggccca taggggtatc tcatgccacg gccgggcatc
     1021 agcctctgcc cgccacgccc gcttatcgtc catgggcata ggcaggtgaa acccttgcac
     1081 ggcaatctgc ggcttcacgc cgtagcgggc ttccagcccc ttgagtcgcc gggcccagcc
     1141 gcccacatac cggtgtgggc tgtcatgaga agacgggaag ttggaatagt acgggtcggt
     1201 ggccacgcca tgtaccgttc gttctgcggc taaagattcg agcaccttat agccatgctc
     1261 cgtcgatatc atgcacaccg tactgcggat ggacagccgg ccggcatagt cggccatcag
     1321 gcggaccagc tcgcgttctt tgccctggca gcagctgcag gggttggggt tctccacgtt
     1381 ggcgaaattc ggctcgtcga tgaacaagcg gtcaaagcct aacgcttgca cgtaatcgat
     1441 agcatcactc atcaggctgc ggaagtccgg attattcagg caggcctgct gcgacggatc
     1501 ggcagcaaag gccgatggtg gctcaccgcc cagcaccttg gcaaatcccc acagatcagc
     1561 ctcgcagctg agtccttgct gcttggtatg ctcgacgacc ggacgcacgg acatgtcgcg
     1621 ccgtcgcagc atatcttcgg ttacgcagaa cagcacggta tcgaagtcgt ggcggcgcat
     1681 ttcgtccaca tcggcggtca gccagtgctc cgtcgggtag gcacacattg cctcaggggt
     1741 aggataattt gaacgctgca tcaatctgtt tataccacgg tagctgaacg gtatgcgtaa
     1801 ggtagcttac atagaaaaac agaggaaacc ataggaatcc tctgtttgtc gctggctcta
     1861 ggctacgttc agcaggtgct cgatcggcga caagaagaag tagacgacga acagcagcgc
     1921 gatgacactt agcagccagt gggtcttgcg gaaccggccg ttgactatcc gcagcagcac
     1981 ataggcgatg actgcggcac cgatgccgtt cgtgatggag taggtgaacg gcatgagcac
     2041 tatggccagg aaggccggga tagcgacttc gtaatcttcc cacggaatgt tacgcacctg
     2101 ggtagccaac aggaagccga cgatgaccag tgccggagtg gcggcttggc tcggaacaat
     2161 agtggttaac ggtgtaagga acagggcgac cacgaacagt ccgccggtca cgatgctggc
     2221 gacacccgtg cgggcgcctt cgccgacacc ggaagctgat tcaatgaacg ccgtgttgga
     2281 gctggacgaa cccaggccac cggcgatggc agcgaagcca tcgacgaaca gcgcccggtt
     2341 gatgaccggt aggcgcccct ttttgtcgag caggccggct tcgttggata cgcctacgat
     2401 ggtgcccata gcatcgaaga agtcactcag g
//
LOCUS       DASSFD010000138         7327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1194303, whole genome shotgun sequence.
ACCESSION   DASSFD010000138 DASSFD010000000
VERSION     DASSFD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194303"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFH39_03250"
     CDS             <1..1248
                     /locus_tag="VFH39_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HET6924819.1"
                     /translation="LLGLYGSRIYEKRFSELGRLFVGSLVGMLALISFSYIANVRIFP
                     ARLVVLYAFLLAFLFLLLFRNLARGIRRELFGYGLGINNVLIVGDTKVTDQLIESLRD
                     TNETGYRVVGVVGGVKHKLTKHESQHVFVSFDEAVHSPAARQLHTIIQTELYSSVEKN
                     NEILTYAQEHHVAYRFVPGNSELFVGNLDVDLFNSIPVIVVHQTALVGWGRVVKRLSD
                     IILGSLGIIIASPLMLLTIIAIKVTDPKGPVFFRVGRLTRFGNRATIIKFRTHKQAFN
                     GLSPEEAFVKMGRPELAKLYRESGDQLPNDPRVSWVGRFLRTYSLDELPQLFNVVKGD
                     ISFVGPRALDPFELEKYAQKNLILSVKSGLTGLAQISGRRDISFEERRQLDLFYVQNW
                     SFWNDLVIIAKTVSVVLFHRGAI"
     gene            1249..2379
                     /locus_tag="VFH39_03255"
     CDS             1249..2379
                     /locus_tag="VFH39_03255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6924820.1"
                     /translation="MAAKPKVAIVCDWLMGTGGAERVVLELHRMYPEAPIYTSQYDRS
                     PELWYHDDWFGDADVRTTWLQRLPKGLKKFLPVLRAWTFSRLDLSQYDLVISCSGAEA
                     KGVRTGKNTLHVNYCHAPTHYYWSRYDQYLKHPGFGRLDWLARWGLRTLVGPLRQWDR
                     QASKRPDIMVAISVHTQEQIRKYYRRDSVVVHPPVDLRRFKPAKPVQRHGFVTVGRQT
                     PYKRIDLAVAACSELKMPLIVIGTGPEHKKLIKMAGRSVTFLTNVSDDQIAAHFHSSL
                     GFIFPTNVEDFGVTGVEALAAGIPVIAYAKGGPRDYIVPGKTGEFFDRQTVESLKSVL
                     HNFKPERYKQDVLTAKAAEFAPAVFRERLQAVVTDALAAREA"
     gene            complement(2376..2561)
                     /locus_tag="VFH39_03260"
     CDS             complement(2376..2561)
                     /locus_tag="VFH39_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924821.1"
                     /translation="MIKNRRPRDAHTHALDRVLYLLEEQSFQNLAMDDERFASFIEQL
                     YQRVETEQQTLSSHPAH"
     gene            complement(2677..3729)
                     /locus_tag="VFH39_03265"
     CDS             complement(2677..3729)
                     /locus_tag="VFH39_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6924822.1"
                     /translation="MKRRIRNVVTAPWFRLAGTYLAIIMLMSIGFSVVFYRTSWNELG
                     RQIPTHADYDHDIGLDFGLATDNAASPTDPSGNVRQFLQTRAQEGRAELLQRLIVLNG
                     LALVLGSALSYYLARRTLRPIEAAMEAQSQFVSDASHELRTPLTALQATNDVALRDPK
                     LTLTKAKSVIQANTEEAVKLQALSNALLNLARQDNSKLALADVSLQAVVSQALTRVVQ
                     PAQSKDISIEDRTPDIKLRAEPHALEQILVILLDNAIKYSPAGSTVYLEGRRRGKYGY
                     VSVRDEGAGIRASDLPHIFRRFYRADTSRTDGAAHGYGLGLSIAHKLVERLGGGISVQ
                     SEPGKGSTFIVRLPAA"
     gene            complement(3726..4400)
                     /locus_tag="VFH39_03270"
     CDS             complement(3726..4400)
                     /locus_tag="VFH39_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6924823.1"
                     /translation="MRILVVEDEHRIAQAVKEGLEQEGYAVDVCYDGDDGYHTAAADD
                     YDAILLDVMLPGMDGYAVCRKLRDGGNHTPVLMLTARDQPRDVVQGLDTGADDYLAKP
                     FSFEVLLARLRALLRRPQNTLGSVLEAGDLKLDTIGKSVTRSGKPISLSSKEFALLEY
                     LLRNKNQVLSKNNIMSHVWDFDADILPNTVEVFMTYLRAKVDKPFKGPNLIVTVRGFG
                     YKIEAA"
     gene            complement(4464..6968)
                     /locus_tag="VFH39_03275"
     CDS             complement(4464..6968)
                     /locus_tag="VFH39_03275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6924824.1"
                     /translation="MRIPLHKTAVGLVVSLLVGVAGMHIVRADQPGWLSSSDPKLSKV
                     IDLPSNQSPGMSNRDCTPLSYSAVDPTYGTKSAKTACFGHSILGFMGLAGSPYIADNS
                     SIGMNMVGYGNNQYLLQPIPNQAMVLSTTSAPVQGAYLHFYTDVTQHLTTLYSQMNDT
                     EYGYAVNALPDFDLRDKNNALIPANISGTLSYSSDGAWMLIDAYGRGFLRVNLATFDV
                     LPFSQSLTGVNDYSSQIGLTAISDDGRYAVIASDNYHFFKVYDLSTCTGTTDASYSKP
                     LNCQYRDYWPYMNSALSGFRALYAVRFTNDDNLLVTASANYQSPTSYTVAKYRLTAAG
                     TAAHSLEYLALGDSYVSGQGEFVYKYGTDTDTNTCHLSPLSYPLTVGAGLFDGYESVA
                     CSGARTEDILNSSDDYTGQVTDHIKQRDRDKEGILAGFSPGMLNQLQFVKRYQPAVVT
                     LSVGGNDIGFADKVKACAVPALSNIPAMNDTLTCFDTYDERMQVVQAIGSAYASLRET
                     YSEILQADPGVRLYVTGYPQVVTEGNCADNVHLNADEIQFAHQLIAYLDWTIERAANS
                     AGARYVNMQNALAGHRLCETTGSNIAINGITAGNDSGAFGLKFLGSESFHPNVLGHHL
                     LGQTLLQQTDNLAEPMPAPDSAITRPVPSDQLAQALLDNLVYTGNPPPDIATASNNFM
                     TGFAVRGQQTPVSVEGLPNDLAPNSNYQVSIHSDTQQLGTLTSDASGNLNGQVVLPVD
                     LLPGFHTVDITGKTRSGETIDMFEIVYVAASADDYNGDGTLNTNDTCLIVPPSGRDAD
                     QDGIDDACDPVIGNPPIKTYPVTVSLTGNTIVVKVP"
     gene            complement(7029..>7327)
                     /locus_tag="VFH39_03280"
     CDS             complement(7029..>7327)
                     /locus_tag="VFH39_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924825.1"
                     /translation="RAAARDLALNIEARLTTNDKINNPLKQNAYYSVNSPPTDIITRS
                     FIFNGLSCSRDYIYFTASDNLAANGYVTLKLNNSSRGLMVELSCALVPALQNYY"
BASE COUNT         1569 a         1954 c         2063 g         1741 t
ORIGIN      
        1 ctgcttggcc tctatggtag tcgcatctac gagaagcgct tcagtgaact cggtcgtctt
       61 tttgtgggca gtctcgtcgg catgctggcg cttataagct tcagttatat cgccaatgtc
      121 cggatctttc cggcgcgtct cgtggtgttg tacgcctttc tgctggcgtt cctgttcctg
      181 ctgcttttcc gtaatctggc ccgcggtatc cggcgcgaac tgttcggcta cggcttgggt
      241 atcaacaatg tcctgatcgt gggcgacacc aaagtgaccg atcagttgat cgaatcgctg
      301 cgcgatacca acgagaccgg ttaccgggtc gtcggagtgg tcggcggggt caaacacaag
      361 ctgactaaac acgaatcaca gcatgtattc gtcagtttcg acgaagcggt gcattcaccg
      421 gcagcccgcc agctgcacac cattatccag accgaactgt attccagtgt cgaaaagaat
      481 aacgagattc tgacctatgc ccaggagcat cacgtggcct accgttttgt gccaggcaac
      541 agcgagctgt tcgtcggcaa cctggatgtc gacctgttca acagcattcc ggtcatcgtc
      601 gtgcaccaga cggcgctggt cggctggggc cgggtggtga agcggctgag cgacatcatc
      661 ctcggcagtc tcggcatcat catcgcgtcg ccgctcatgc tgctgaccat catcgccatc
      721 aaggtcaccg atccgaaggg tccggtattc ttccgtgtcg gtcgtctgac ccgcttcggt
      781 aatcgggcta ccatcatcaa gttccgtacc cacaaacagg ccttcaacgg tctgtcgcct
      841 gaagaggctt ttgtgaagat gggccgtcct gaactggcca agctgtaccg cgaaagtggc
      901 gaccagttac caaatgaccc ccgcgtcagc tgggtgggcc gcttcctgcg cacctacagc
      961 cttgacgaac tgccgcagtt gttcaacgtg gtaaagggcg acatcagctt cgtcggaccc
     1021 cgggcactcg atccgttcga actcgagaaa tacgcccaga agaacctgat cctgtcggtc
     1081 aaatcgggtc tcaccggctt ggcgcagatc tccggccggc gcgacatcag tttcgaggag
     1141 cggcgccaac tcgacctgtt ttacgtgcaa aactggagct tctggaacga tctggtcatc
     1201 attgccaaga ccgtatcggt cgtattgttt caccgcgggg caatctaaat ggcagccaag
     1261 ccgaaggtag cgatcgtctg cgactggctg atgggcaccg gcggtgccga gcgggtggtg
     1321 ctggagttgc accgcatgta cccggaagcg ccgatatata cgtcgcaata cgaccgctcg
     1381 ccggaactgt ggtatcacga tgactggttc ggtgatgccg atgtccggac gacttggctg
     1441 cagcgattgc cgaaaggact gaagaagttt ctgcccgtat tgcgggcctg gacgttcagc
     1501 cggctcgatc taagccagta cgacctggtt atttcgtgca gcggtgcgga ggcaaagggc
     1561 gtacggaccg gcaaaaatac tttacacgtt aactattgtc acgcaccgac tcattattac
     1621 tggagccggt acgatcagta cctgaagcac cccggtttcg gccgcctgga ctggctggct
     1681 cgctggggct tgcgcacact cgtcggaccg ctgcggcaat gggaccgcca agcctccaag
     1741 cgtccggata tcatggtcgc catttccgta catacgcagg aacaaatccg taaatactat
     1801 cgacgcgact cggtcgtggt gcatccaccg gtcgatctgc gacgcttcaa accagccaag
     1861 ccggtacagc ggcatggctt cgtgacggtt ggccgccaga ccccgtacaa gcggatcgac
     1921 ttggccgtag cggcctgcag cgaactgaag atgccgctta tcgtcatcgg taccgggcct
     1981 gaacacaaga aactcattaa gatggccggc cgcagcgtga cctttctgac taacgtgagc
     2041 gatgaccaaa tcgccgcgca ttttcattca tcactcggct ttatatttcc gacgaatgtc
     2101 gaggacttcg gagtgaccgg cgtggaagcc ttggcggctg gcataccggt catcgcctac
     2161 gccaaaggcg gcccgcggga ttacatcgtg cccggcaaga ccggtgaatt cttcgaccgg
     2221 cagaccgtgg agtcattaaa aagcgtgcta cataatttca agccggaacg gtacaagcag
     2281 gatgtgctga ccgccaaagc tgcagaattc gcacctgctg tcttccgcga gcggctgcag
     2341 gcggtcgtta ccgacgcact tgctgcccgg gaagcttaat gagcggggtg gctcgaaagc
     2401 gtttgttgct cggtttcgac ccgctgatat aactgttcga tgaagctggc gaaacgctca
     2461 tcatccatcg ccaggttctg gaaggactgt tcctccaaca ggtagagcac acggtcgagt
     2521 gcatgtgtgt gagcgtcgcg tggacgtcgg ttctttatca tgatgacctc cttcctggag
     2581 cttaattaat agcgaaacca acagttaaca agctaagcgt taaccttagt acttatccag
     2641 tgtagcgagc aaacattagg cgcgccttaa aaaatttcac gctgccggca aacggacgat
     2701 aaatgtcgag cctttgcccg gttcgctctg gacgctgatg ccgccgccga ggcgctcgac
     2761 aagtttatgc gcgatggaga gccctagccc gtagccatgc gccgcgccgt cggttcgcga
     2821 ggtgtcggct cggtagaaac ggcggaagat atggggcagg tcggatgcac ggataccggc
     2881 accttcgtca cgtacgctca catagccata tttgccgcgc cgccgccctt ccagatagac
     2941 cgtactaccg gccgggctgt acttgatggc attgtcgagc aggatgacca gaatctgctc
     3001 gagggcatgg ggctcggcgc gcagcttgat gtcgggtgtc cggtcttcaa tgctgatgtc
     3061 tttgctttgg gctggttgta ctacacgggt aagggcctga gagactaccg cctgtaagct
     3121 gacatccgcc agcgccaact tactgttatc ctggcgcgcc aggttcagca gggcgttaga
     3181 cagtgcctgc agcttaacgg cttcttcggt gttggcctgg atgaccgatt tggctttggt
     3241 aagtgtcagt ttgggatccc gcagggccac gtcattggtg gcctgcagag cggtcagggg
     3301 ggtgcgcaat tcgtgcgagg cgtcgctgac gaactggctc tgcgcttcca tggcggcttc
     3361 gatggggcgc aatgtgcggc gagccaggta atagctcaat gcgctgccga gcaccagagc
     3421 caaaccgttc agtactatca gccgctgcaa cagctccgcc cggccttctt gggcgcgggt
     3481 ctgcaggaac tgacggacat tgccggacgg gtcggtgggg gatgccgcat tgtctgttgc
     3541 cagcccaaag tccagcccaa tgtcgtgatc gtaatcggca tgggtcggga tctggcgtcc
     3601 caattcattc caggaggtac ggtaaaacac cacactgaag ccgatactca tgagcatgat
     3661 gatcgccagg taagtcccgg ccaggcggaa ccatggtgcc gttacgacgt tgcgtatgcg
     3721 tcgtttcatg cggcctctat cttgtagccg aagccgcgca ccgtcacgat caggttcgga
     3781 cctttgaacg gcttgtcgac cttggcgcgc aggtaggtca tgaacacctc gaccgtgttc
     3841 ggcaatatgt cggcgtcgaa atcccacacg tgcgacatga tgttgttctt gctcaagacc
     3901 tggttcttgt tgcgcagcag gtattcgagc aaggcgaatt ctttgctgct gagactgatg
     3961 ggcttaccgg accgtgttac cgatttgcca atcgtgtcga gcttgaggtc cccggcttcc
     4021 agtaccgaac cgagcgtgtt ctgcgggcgg cggagcaggg cgcgcagccg ggccagcagt
     4081 acctcgaagc tgaacggctt ggccaggtag tcgtcggcgc ctgtgtccag gccttgcacg
     4141 acatcgcgcg gctggtcgcg ggcggtgagc atcagcacgg gtgtgtggtt gccgccatcg
     4201 cgcagtttgc ggcacaccgc gtagccgtcc atgccgggca gcatcacatc cagcaagatg
     4261 gcgtcgtagt catccgcggc cgcggtgtgg tagccgtcat cgccgtcgta gcatacgtcc
     4321 acggcatagc cttcctgctc caggccttct tttactgcct gggcaatccg gtgctcatct
     4381 tcgacgacca gtattctcat ttactcctta agtgtacccg cccgcacctt aaattccact
     4441 taaatatctc tctttggcag tccctacgga acttttacga cgatggtgtt accggtcagg
     4501 ctcacggtga cggggtaggt ctttatcggc gggttgccaa taaccggatc acaggcatca
     4561 tctatgccgt cttggtccgc gtcgcggccg ctcggcggaa ctatcaagca agtatcattc
     4621 gtattcagcg taccgtcacc gttgtagtca tcggcacttg ctgccacata cacgatttcg
     4681 aacatatcaa tcgtctcgcc actgcgggtt ttgccggtaa tgtcgaccgt atggaaccct
     4741 ggcagtaagt cgacaggcaa gacgacctga ccattcaaat taccgctggc gtcactcgtt
     4801 aaagtgccga gttgttgggt gtctgagtgg atgctgacct ggtagttaga attaggtgct
     4861 aagtcgttag gcaagccttc taccgatact ggtgtttgtt ggccgcggac ggcaaaacca
     4921 gtcatgaaat tattgctcgc agtggctata tcgggcggcg ggttgccggt gtacaccaga
     4981 ttatcgagca gtgcctgggc gagttgatca ctcgggacgg ggcgggtgat tgcgctgtcc
     5041 ggtgccggca ttggttcggc caagttatcg gtttgttgca gtagtgtttg ccctaatagg
     5101 tggtggccta atacgtttgg atggaaactt tctgatccta agaacttcag gccaaatgcc
     5161 cctgagtcat tgccggcagt aatgccattt atagctatat tactgccggt cgtttcgcac
     5221 aaacggtggc cggctaaagc gttctgcatg ttgacgtagc gagctccagc gctgttggcc
     5281 gctcgctcta tggtccagtc taagtaagcg atgagttggt gcgcgaactg gatttcgtct
     5341 gcattcaaat gcacattgtc ggcacaattg ccttcggtta cgacttgcgg atagccggta
     5401 acatataatc gcactcccgg atcggcctgc aggatttctg agtaagtttc acgcagtgat
     5461 gcgtatgccg agccgatcgc ctggacgacc tgcatacgtt catcgtaagt gtcgaaacaa
     5521 gtgagcgtat cgttcatggc gggtatgttg ctcagtgccg gtacagcgca ggcctttact
     5581 ttatccgcaa accctatatc gttaccgcct acggataaag taaccacagc gggctgatac
     5641 cttttgacaa attgtagctg gttcaacatg ccgggagaaa aaccagctag tattccttcc
     5701 ttgtccctgt ccctttgttt aatatgatct gttacctgcc cagtataatc gtctgatgaa
     5761 tttaatatgt cttccgtcct agcccctgag caggcgactg attcgtagcc atcgaatagc
     5821 cctgcaccga ccgttaacgg atacgacaaa ggggaaagat ggcaggtatt agtatctgta
     5881 tctgtgccgt acttatacac gaattcgcct tgaccggata cgtaggagtc gcctagggcc
     5941 agatattcca gggagtgagc ggcagtacct gcggctgtca aacggtactt cgcaacggta
     6001 tagcttgtag ggctttggta atttgccgat gcggtaacga gcaaattatc gtcattagta
     6061 aagcgtaccg catacagtgc gcggaagccg ctaagcgcgc tgttcatgta aggccaatag
     6121 tcgcgatact ggcagttcaa cggtttgcta taggacgcgt cagtcgtgcc ggtacaggtc
     6181 gataggtcgt acaccttgaa gaagtggtaa ttatcgctgg caatgactgc gtagcgccca
     6241 tcatcactta tggccgtaag ccctatctgc gagctgtagt cattaacgcc ggtaagggat
     6301 tggctgaatg gtagaacatc aaaagttgcc aaattcacgc gcagaaaacc ccgtccatag
     6361 gcatctatca gcatccaggc cccatcgctg gaatagctta aggtgccgga gatgtttgcc
     6421 ggaatgagcg cattattttt gtctcgtaag tcaaaatccg gcagagcatt tactgcatat
     6481 ccgtattccg tgtcgttcat ctggctataa agagtagtga ggtgttgcgt gacgtccgta
     6541 taaaaatgga gataggcgcc ttgcacgggt gcgctcgtag tagatagcac catagcttgg
     6601 ttaggtatgg gttgtaacag gtattggttg ttaccatagc ccaccatgtt cataccaata
     6661 gagctgttat cggctatata tggtgaaccc gcgagcccca taaaaccgag aatactatgg
     6721 ccgaagcagg ctgtcttcgc gcttttggtg ccatatgtcg gatcaacagc actgtaagag
     6781 agaggggtgc agtcgcgatt actcataccg ggcgactgat tgcttggcaa atcgattact
     6841 ttgctgagtt tagggtcgga gctgctgagc cagccgggct gatccgctcg tactatgtgc
     6901 attcccgcaa cgcccaccag taaactaacc accaggccca ccgctgtttt gtgaagcgga
     6961 atgcgcaaaa cagtcaccaa ttactaatca ataacagcaa ttgtacatat gtaccaataa
     7021 attacaaatt aataatagtt ttgcagtgca ggaacaaggg cacaacttaa ttccaccata
     7081 agccccctgg aagagttatt taacttgagt gttacatagc cattagccgc taggttatca
     7141 cttgcagtga agtatatgta atcgcgagag caacttaagc cattgaatat aaatgagcgc
     7201 gttatgatat cggtcggagg gctattcacg gaataataag cattctgctt tagtggattg
     7261 ttaatcttgt cattcgtagt tagccttgct tcgatattta atgctaagtc tcttgcggcg
     7321 gctctat
//
LOCUS       DASSFD010000139         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_817854, whole genome shotgun sequence.
ACCESSION   DASSFD010000139 DASSFD010000000
VERSION     DASSFD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817854"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFH39_03285"
     CDS             complement(<1..633)
                     /locus_tag="VFH39_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6924826.1"
                     /translation="MSTSKKNVGALAHKSGVSFRVWAPFADSVAITGTFNDWQLTSLD
                     SESDGYWTVTVKGAQAGQEYKYVIMHGEEMFEKNDPRALQLTTVAGNSVVVDPEFDWG
                     STAPQAVPHNHQVIYELHVGTFNRTDPATPGTFATAQAKLDYLKDLGVTTIELMPVGS
                     MSMEREWWGYTPEYIYAVENLYGGRHQMLEFIREAHARGMAVILDVVYNHF"
     gene            788..1051
                     /locus_tag="VFH39_03290"
     CDS             788..1051
                     /locus_tag="VFH39_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6924827.1"
                     /translation="MSNPRRTVLYIGVTNNLDRRVAEHKSHDVPGFTHRYNCTDLLYF
                     EDTPPVEAAIAREKELKKWSRSKKEALIAGLNPDKRDLSLQSR"
     gene            1119..2552
                     /locus_tag="VFH39_03295"
     CDS             1119..2552
                     /locus_tag="VFH39_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET6924828.1"
                     /translation="MVFRRTTLLLFGFIAFLLGVGLARTNHLIDSSWPCLALGVCIFV
                     FRKKNVWALVWVVLFALSLGWWRGGIYMQKLAGWQHYYGHKVSITAVAMDDGVYGKNS
                     QMSFDVNNVVLDGRKLTGKMTLSGFGLNAVFQGDEVRASGKLQSTLGAAQGRMSFAQI
                     KLAGHHPSLIADLRRKFAAGMQNALPEPLASFAMGLLIGQRATLPVNVKQDLVMVGLT
                     HIIAVSGYNLTIMLRASRGIFGRRSKRISTLLSLALIGVFLLFAGSSASIVRAAIVSV
                     LSIAAVYYGRQVKPLLLIAMAAAITAYANPFYVWTDTSWYLSFLAFYGVMVLAPMVAA
                     RLHLRWQNSLVAMVALESLCAEVMTLPFVLYTFGQMSFIGLVANVMVVMLVPLGMLLS
                     LVAGLAGMLAAPLAGWFAWPARLLLTYMLDIAHLLARLPHIFVQNLSLPSAEMIGAYL
                     LVALMTWVLWNKTKHLKNDTITEEIKF"
     gene            2649..3389
                     /locus_tag="VFH39_03300"
     CDS             2649..3389
                     /locus_tag="VFH39_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6924829.1"
                     /translation="MSGHSKWSTIKREKGAKDAARGAVFTKLGNAIAIAARSGADPET
                     NFALRLAMDKAKAANMPSANIQRAIDRVKDKNAAQLQEVLYEGYGPGGVAILVECATD
                     NVNRTYPDVRLAFSKHGGNIAEKGAVAFQFDRKGMVRVKGGGDEVLMAALEAGADDVQ
                     EEPAADGKESVIYTDPKELAKVRDTLKDSGLEVTEAELTFVPNVTVEVTDKDTAGKIM
                     RLMDALEDIDDVSNTHVNFDIPEELLST"
     gene            complement(3378..>3600)
                     /locus_tag="VFH39_03305"
     CDS             complement(3378..>3600)
                     /locus_tag="VFH39_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924830.1"
                     /translation="FLSVVAIVNGQPAAIPASTTTGPPISRLILLWPLYTALIAIVTS
                     FWLGERRERQLILAHPLRFHLEPEHRLGT"
BASE COUNT          796 a          944 c         1073 g          787 t
ORIGIN      
        1 gaaatggttg tataccacgt ccagtatgac agccatgccg cgggcgtgcg cctcccgtat
       61 gaattcaagc atctggtgcc gcccgccgta caggttttct accgcgtaaa tgtattcggg
      121 tgtgtagccc caccattccc gttccatgga catactgccg acaggcatga gctcaatagt
      181 ggtaacaccc aggtccttca ggtaatccag cttggcctgg gcagtagcga atgtcccggg
      241 tgtggccggg tcggtacggt tgaatgtgcc gacgtgcagt tcgtagatga cctgatggtt
      301 atgcggtaca gcctgcggcg cggtagagcc ccagtcaaac tccgggtcca ccactaccga
      361 gttgccggcg accgtggtca gctgcagggc acgcggatcg ttcttttcga acatctcttc
      421 gccgtgcatg atgacatatt tgtactcctg cccggcctgg gcgcccttga cggtaactgt
      481 ccagtagccg tcgctctcgc tgtccaggga ggttaactgc caatcattga aagtgccggt
      541 tatggcgacg ctgtcggcga acggcgccca cacccgaaag gacacgcccg atttgtgcgc
      601 cagcgccccc acatttttct tagacgtact catgatctta ctgttattat ccttacgctt
      661 aagccaaagc gcaaccattt atactcatgg ttacatgaat cagtggactc cggtattgtc
      721 atctcgaacg aagagagacc ccgttcacct aagcaaaggc tctcatgtac acatattggg
      781 ttatatcatg tctaacccgc gccgtaccgt cctttatatc ggcgtaacta ataatctgga
      841 caggagagtg gccgaacata aatcgcacga tgttcccggt ttcacgcata ggtataattg
      901 cactgatttg ctctactttg aagatacgcc cccggtcgaa gctgcgattg cccgcgagaa
      961 agagttaaag aagtggagcc gcagcaagaa agaagcattg attgcaggcc tgaatccgga
     1021 caaacgggat ctctccttgc agtcgagatg acactggccg cttaaagctg ctttgatgct
     1081 catggttggc gcgattactg ctgttacaat tgccgccaat ggtattccgg cgcacgacat
     1141 tactgctgtt tggtttcatc gcgtttctgc ttggggttgg gctggcccgc accaatcatc
     1201 tgattgattc cagctggccc tgtctggcgc taggtgtctg tattttcgta ttccgtaaaa
     1261 agaatgtgtg ggctttagtg tgggttgttc tgtttgccct gagcctgggt tggtggcggg
     1321 gcggcatata tatgcagaag ctggccggct ggcagcacta ttatggccat aaagtcagta
     1381 tcactgcggt ggcgatggac gacggcgtgt acggcaagaa ctcgcagatg agcttcgatg
     1441 tgaacaatgt ggtcctagac ggccggaagc tgacaggcaa gatgacgctt agcggtttcg
     1501 ggctgaacgc ggtgttccag ggcgatgagg ttagagccag cggcaaatta caaagtacgc
     1561 tgggggcggc acagggcagg atgagttttg cccagataaa actggcgggg caccatccgt
     1621 cgttaattgc ggatttgcgg cgtaaatttg ccgccggcat gcagaacgcg ctgccggaac
     1681 cgctggccag ctttgccatg gggctgctga taggccagcg ggcaacactg ccggtaaacg
     1741 taaagcagga cctcgtgatg gttgggctga cacacatcat cgccgtatcg ggctacaacc
     1801 tgacaattat gctgcgggcc agcaggggca tcttcggtag gcggagcaaa cggataagta
     1861 cgctgctgtc acttgcgctc ataggcgttt tcctgctgtt tgccggcagc agcgccagta
     1921 tcgtgcgggc agcgatagtt agcgtgttaa gtatcgctgc cgtctactac ggccggcaag
     1981 taaaacctct gttactcatt gctatggcgg cggccatcac cgcttacgcc aacccgtttt
     2041 acgtgtggac cgataccagc tggtacctga gcttcctggc tttttacggc gtgatggtgc
     2101 tggcgccgat ggttgccgcg cgcctgcatt tacggtggca gaactcactg gtagccatgg
     2161 tggcactgga gagcttatgc gccgaagtaa tgacgttgcc cttcgtgctg tataccttcg
     2221 ggcagatgtc gttcatcggc cttgtagcga acgtgatggt agtaatgttg gtgccgctgg
     2281 gcatgctgct gtcgctggtg gcgggcctgg cgggcatgtt ggctgcaccg ctggccggct
     2341 ggtttgcctg gccggcccga ttactgctga cgtacatgct cgatatcgct catttgctgg
     2401 ctcgcctgcc gcacattttt gtccagaacc tgagcttacc gtcagcggaa atgatcggag
     2461 cgtacctgct ggtcgccttg atgacatggg tgctctggaa caagacaaaa catcttaaaa
     2521 atgatacaat aacagaggaa attaagttct aacgcgtcat tccgaactcg tttcggaatt
     2581 ccccaaaaag caaacgggat tctgaaatga attcagggtg acggcacgag aggataaaat
     2641 acaggaagat gtccggtcat tcgaagtgga gtacgatcaa gcgcgaaaag ggcgccaagg
     2701 atgcggcccg cggcgcggtg ttcactaagc tcggcaacgc catagccatt gccgcgcgca
     2761 gcggcgccga cccggaaacg aactttgcgt tgcgcctggc tatggacaag gccaaggccg
     2821 ccaatatgcc gagcgccaac atccagcgcg ccatcgatcg cgtgaaggac aagaatgccg
     2881 ctcagctgca ggaagtgctg tacgagggct acgggcccgg cggtgtggct atactggtgg
     2941 aatgcgccac cgacaacgtc aaccggacat atcccgatgt ccgcctggcg ttctccaagc
     3001 acggcggcaa catagccgaa aagggtgccg tagccttcca gtttgaccgc aagggcatgg
     3061 tacgggtcaa aggaggcgga gacgaggtgt tgatggccgc cctggaagcg ggcgccgacg
     3121 atgtacagga agaacctgcc gccgacggaa aagagtcagt tatatacacc gaccccaagg
     3181 aattggccaa agtacgcgat acgctcaaag acagcggcct ggaagtaacc gaagccgagc
     3241 tgaccttcgt gccaaatgtc acggtggagg tgaccgacaa agataccgcc ggtaagatca
     3301 tgcgcctcat ggacgccttg gaagatatag acgacgtcag caatacgcat gtcaattttg
     3361 atattccaga ggaactgcta agtacctagg cggtgctccg gttcgaggtg aaagcgcagc
     3421 ggatgagcca gtataagctg ccgctcgcgg cgttcaccaa gccagaagct ggtgacgata
     3481 gcaatgagcg ccgtgtagag cggccagagc agaatcagcc ggctgatagg gggcccagta
     3541 gtggtgctcg ccggtatggc tgccggctgg ccgttgacga tggcgacgac actcaggaaa
//
LOCUS       DASSFD010000140         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_189731, whole genome shotgun sequence.
ACCESSION   DASSFD010000140 DASSFD010000000
VERSION     DASSFD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189731"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFH39_03310"
     CDS             <1..371
                     /locus_tag="VFH39_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641479.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET6924831.1"
                     /translation="VALAAEQHHDDVEATSVEALIVRVCDAISASRPGARNISAENFV
                     ERMKDLENVANSFNGIEKSYAISAGREVRVFVKPQQIDDLSAIKLARDIATKIESSMQ
                     YPGTIKVNVIRETRAVEFAK"
     gene            442..1239
                     /locus_tag="VFH39_03315"
     CDS             442..1239
                     /locus_tag="VFH39_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET6924832.1"
                     /translation="MNILYVGDIYGRPGIEVVQQVLPRLRQEKQIDFVVAQGENVSNG
                     RSLLPADMQTLQAAGVDFFTGGNWSLFSPEAAPLLSDPNVPAIRPANYPEGTPGLGWK
                     VASTPRGKVLVISLLGDVVGRDAGKAIDNPLLVVDKILAENQELERVAAIVNLHGDFS
                     SQKVIIGHYLDGRVTAVVGDHWHVPTADARVLPMGTAHMSDVGMCGTLDSSLGVKLSV
                     VIPRWRDGKVSKNEIETEGPRQFNALLIHSDDGTGLARGVEPVRQII"
     gene            complement(1236..1589)
                     /locus_tag="VFH39_03320"
     CDS             complement(1236..1589)
                     /locus_tag="VFH39_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924833.1"
                     /translation="MLTSLGEQERIAKLRRLSATERQSVEEAYDKFIAEGSQSLNRFL
                     STMACGKLLELESDLSQLALEAKENKESLDPDQQAILDSLAMVIMSRAIIDEVHLAPL
                     PVWERPFEQEHLTAA"
     gene            1851..1927
                     /locus_tag="VFH39_03325"
     tRNA            1851..1927
                     /locus_tag="VFH39_03325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1885..1887,aa:Pro,seq:tgg)
BASE COUNT          434 a          558 c          597 g          482 t
ORIGIN      
        1 ctgtcgctct tgccgcggag cagcaccacg atgacgttga ggcaacgagc gttgaagctt
       61 tgatcgtccg cgtctgtgac gccatttcgg catcccgacc tggcgcccgc aacatcagcg
      121 ccgagaactt cgtggagcgc atgaaggact tagagaacgt cgctaacagc ttcaacggca
      181 tcgagaagtc gtacgccatc agcgccggtc gcgaagtgcg cgtgttcgta aaaccgcagc
      241 agatcgatga tctcagcgcc atcaagctgg ctcgcgatat cgccaccaag atcgaatcaa
      301 gcatgcagta ccccggtact atcaaggtaa acgtcatccg cgaaacccgc gccgtcgaat
      361 tcgccaagta acgggctttc gtcatgccgc ctttagccgc ctggcgcccc ggccgcttac
      421 gcggctgcta tacttagcag tatgaatatt ttgtacgtcg gagatatata cggccggccg
      481 gggattgagg tggtacagca ggttttgcca cggttgcgcc aggaaaagca gatcgatttc
      541 gtggtggccc agggcgaaaa tgtgagcaac ggacgcagct tgctgccggc cgacatgcag
      601 acattgcaag ccgccggcgt ggactttttt accggcggca actggagctt gttcagccct
      661 gaggcggcgc cgttgttgtc ggacccgaat gttccggcca tccgcccggc caactatccc
      721 gagggtacac cgggtctggg atggaaggtg gcttccacgc cccgcggcaa ggtgctggtt
      781 atcagtttgt taggtgatgt ggtgggccgc gacgccggca aagctatcga taacccgctg
      841 ctggtggtcg acaaaatctt ggccgaaaac caagagctgg aacgcgtagc ggcaatagtc
      901 aacctgcacg gtgatttcag cagccaaaag gtcataatcg gccactacct ggacgggcgg
      961 gtgactgctg tagtcggaga ccactggcat gtgccgacgg ccgatgcccg tgtgctgcct
     1021 atgggaacgg cgcacatgag cgatgtcggc atgtgcggca cgctggattc gagccttggc
     1081 gttaagctaa gtgttgtcat tccgcgctgg cgcgacggta aggtcagcaa aaacgaaata
     1141 gagactgagg ggccgcgcca gttcaacgcc ctgctcatac atagcgacga tggcaccggt
     1201 ttggctaggg gcgtagagcc ggtacggcaa attatctagg ctgctgtcaa atgttcctgt
     1261 tcgaaaggcc gttcccagac gggcagcggt gcgagatgta cctcgtcgat gatcgcccgc
     1321 gacatgataa ccatggccaa actatccagg atggcttgct ggtcggggtc cagactttct
     1381 ttgttttcct tggcttcaag cgccagctgg gacaggtcgc tttccagctc caatagcttt
     1441 ccgcaagcca tggtgctcaa aaaccggttt aaactctgag agccctccgc tataaattta
     1501 tcgtacgctt cttctacgga ttgccgctcg gtagcggaga ggcgccggag tttggcgatt
     1561 ctttcctgct ctcccaaaga agttaacata ctatatatac ccacgttgtg tgatgtaatg
     1621 cgttcggcca tgactaaagt ccattcgtta aactaatagt tgtctccttc tgtattactc
     1681 ctgtgtgccg gctattgtca actgaattat ggataattgt taaagtatag tttctggcgt
     1741 ataatttacc tctgttacat ttgggatctc gcggccgacg cataatgttt gccttgtctg
     1801 cgggcagggc gtttcgttcg tggttgcaaa aagccaagac attggccttt cggggagtag
     1861 cgcagtctgg tagcgcacct ggtttgggac cagggggtcg caggttcaaa tcctgtctcc
     1921 ccgaccagct tactcggtaa ctccagtttc cagagagaaa ctggagtttt tctattgcaa
     1981 cttgcgtatc gggccaggct ttaccctgcg ccgaaactct tttgccgccg gaacctcatt
     2041 gagcttgccc cctgggtagg ggtatcgctg t
//
LOCUS       DASSFD010000141         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1701737, whole genome shotgun sequence.
ACCESSION   DASSFD010000141 DASSFD010000000
VERSION     DASSFD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701737"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..1262
                     /gene="dprA"
                     /locus_tag="VFH39_03330"
     CDS             399..1262
                     /gene="dprA"
                     /locus_tag="VFH39_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET6924834.1"
                     /translation="MDVNTLTVKNGGIPARLHNIPSPPKELFHRGEDLKVLLQRPCVA
                     IVGSRGVTAYGQQVTQHFAGKLAEQGIVVISGLALGVDTIAHRAALAVNGRTISVLPS
                     SVQAPYPAGNRQLAEKILTGGGALVSEYAEGATNFKTNFVARNRLVTGLADALLITEA
                     TADSGSLHTAKFARLQDKPVFAVPGNITSRASVGTNSLIKAGAAAATSYKDVLKALGL
                     QEHRLKAAAVQGANETEQKVLDLLFAGMSDGEALLRESGLEISLFNQTLSMLEITGRV
                     RALGNNQWAIY"
     gene            1354..1524
                     /locus_tag="VFH39_03335"
     CDS             1354..1524
                     /locus_tag="VFH39_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924835.1"
                     /translation="MKLMITIGIFVGGLVGGWLGGLLDGGNWLGGWSLLLSTVGSFVG
                     IWAGYKAGKYYF"
     gene            1679..>2692
                     /locus_tag="VFH39_03340"
     CDS             1679..>2692
                     /locus_tag="VFH39_03340"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HET6924836.1"
                     /translation="MPKNLVIVESPAKAKTIEKFLGKDFTVKSSFGHIRDLPKKGLNI
                     DIANNFEPKYEVSADKRKVVAELKSAAKSAEVWLASDEDREGEAIAWHLTQALKLDPK
                     KTKRIVFHEITKPAIEAAIQKPRTVDLKLVDAQQARRVLDRLVGYELSPVLWKKIRTG
                     LSAGRVQSVAVRLIVDREREIKEFVPESSFKVSAVFITDDTELPAELDKKLSGANEAR
                     DWLEAAKDAEFTVTDIARRPAVRNPGAPFTTSTLQQEASRRLGYSVRQTMTLAQRLYE
                     AGHITYMRTDSTTLSTQALGAAGQYITAQFGKNYHELRQYRTKNASAQEAHEAIRPTD
                     FHTT"
BASE COUNT          665 a          747 c          756 g          524 t
ORIGIN      
        1 gtcggccttt tcaggccaga accgaagttc tggccaccct ttaagcccac ccggtgaaac
       61 agcgacgatc taaataaaaa agtgccgtgt ctccggccac cgaagcggtc agaaccattc
      121 aggctcacac tgattgggca gtcccaccaa aaaccttggg gtcccccatg ctgagcataa
      181 ggtcttaaag gggttagtat gctctagaac gccccgggcg aacccggaag ctaagagtaa
      241 gtggagggta acaacttgac gacctagcac gctctaaagc aatctaacat aattgttaga
      301 attgggggta taaggtgcga taaatctcca tacggagact tacgagttgc tattttagca
      361 agaatatttc tttatgtcaa tagggtataa taattttcat ggatgtcaac acgctcacgg
      421 ttaagaacgg gggtattcct gcccggctgc acaacattcc cagtccgccg aaagagctgt
      481 ttcacagggg tgaagatctg aaagtgttgc ttcagcgccc ctgtgtcgcc attgtcggca
      541 gccgtggcgt aacagcgtac gggcagcagg ttacacagca ctttgccggc aagctggcgg
      601 agcagggaat agtcgtcatc agcggcctgg ccctgggcgt tgatactatc gcccaccggg
      661 cggcgctcgc agtaaacggc cggacgatta gcgtattgcc cagtagcgtg caagcgccat
      721 atccggccgg caatcggcag ctggccgaaa aaatcctgac cggcggcggg gcacttgtca
      781 gcgaatacgc cgaaggtgca accaacttca agaccaattt cgtggcgcgc aaccgtttag
      841 tcaccggctt ggcagacgcc ttacttatca cggaggcgac cgccgatagc ggcagcctgc
      901 atactgccaa gttcgcacgc ctgcaggaca agccggtctt tgccgtaccc ggcaacatta
      961 ccagccgagc cagcgtcggc acgaacagcc tcatcaaagc cggcgccgcc gccgcaacct
     1021 catataaaga cgtcctgaag gccttaggcc tccaggaaca ccggctgaag gccgcggctg
     1081 tccagggcgc aaacgaaacg gaacaaaagg tactggattt attgttcgcc ggtatgagcg
     1141 acggcgaggc gctcctgaga gaaagcggcc tggaaataag cctctttaac caaactctgt
     1201 ccatgctgga aataaccggt agagtgcgcg cactgggcaa caaccagtgg gccatttact
     1261 aatgttcggt ctcgatgaat gagcgaacca ttgacggttc gggggcgcat tggtacttga
     1321 gcattgccaa tcgctaagtg ttacactgga agtatgaagc ttatgattac tatcggcatc
     1381 ttcgtcggcg ggctggttgg cggctggttg ggaggattgc tcgacggcgg taactggctc
     1441 gggggctgga gccttttgct cagcaccgtc ggctctttcg tcggtatctg ggccggctac
     1501 aaagcaggca aatactactt ttagcaaggt tcaatgctca atgaatgagc ggtgccgtta
     1561 atgcattgaa taagtgaacc cctgggcatt cattgacact tgggaactgc gcctttattc
     1621 aatcgtattc ttatatatag gttgcattcc gggcgacaat acgcttaaga tgtaacgtat
     1681 gcctaaaaac cttgtgatcg ttgagagccc cgccaaggca aagactatag agaagtttct
     1741 tggcaaagat tttaccgtga agagcagttt cggccacatc cgcgacctgc ccaagaaggg
     1801 cctgaacatt gatattgcca acaactttga gccaaagtat gaagtcagtg ccgacaagcg
     1861 caaagtagtt gccgagctta agagtgcggc caagagcgcc gaggtctggc tggccagcga
     1921 tgaggaccgt gaaggcgagg cgattgcctg gcacctgacg caggcactca agctcgaccc
     1981 gaaaaaaacc aagcggatcg tcttccacga aataaccaag ccggctatcg aggccgccat
     2041 acagaaaccg cgcacggtgg atttaaaact ggtcgatgcc cagcaggcgc gccgcgtgct
     2101 tgaccggttg gtgggctacg aactcagccc ggtactctgg aaaaagatcc gtaccggtct
     2161 cagcgccggc cgcgtccaat ccgtcgccgt ccgtttaatc gttgaccgcg agagggaaat
     2221 caaggagttt gtgccggaga gctcgttcaa ggttagcgcc gtatttataa cggatgatac
     2281 agagctgccc gcggagctgg acaagaaact ctccggtgca aatgaggccc gagactggct
     2341 ggaagccgcc aaagacgccg agtttacggt tacggatatt gcccggaggc ccgccgtccg
     2401 caatcccggg gcaccgttca ccaccagtac gctgcagcag gaggcctcgc gtcggctcgg
     2461 ctacagtgtg cgccagacca tgacgctggc gcaacggctg tacgaagccg ggcacatcac
     2521 ctacatgcgc accgacagta ccaccctatc cacgcaagcc ctgggggcgg ccgggcaata
     2581 cataaccgcc cagttcggca aaaactatca tgaactgcgg cagtaccgga ccaaaaacgc
     2641 cagcgcccag gaagcccacg aggcaatccg cccgaccgac ttccatacca cc
//
LOCUS       DASSFD010000142         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1052599, whole genome shotgun sequence.
ACCESSION   DASSFD010000142 DASSFD010000000
VERSION     DASSFD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052599"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFH39_03345"
     CDS             complement(<1..235)
                     /locus_tag="VFH39_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924837.1"
                     /translation="MWLRGIASLYGPGLPAALVTRLRTFGYSPFKLLSWFWQAKTYSA
                     PARPNEVEQPFIVFLWLGIVAQIGFGVALLVEWA"
     gene            337..783
                     /locus_tag="VFH39_03350"
     CDS             337..783
                     /locus_tag="VFH39_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HET6924838.1"
                     /translation="MPALDPTTRHAISHTLHCLTGCGIGEVLGSAIGSAAGWPNLWQT
                     VLAVVLAFAFGYGLTYRGARRHGASAADARGTALRTDTVSIISMEIIDNVLEYLIPGA
                     MSAVVASWLFWWSLALSLGVAFVVTVPVNRLMMSRFGIGHGHGAHH"
     gene            827..903
                     /locus_tag="VFH39_03355"
     tRNA            827..903
                     /locus_tag="VFH39_03355"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:861..863,aa:Ala,seq:ggc)
     gene            1035..1199
                     /locus_tag="VFH39_03360"
     CDS             1035..1199
                     /locus_tag="VFH39_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924839.1"
                     /translation="MAKDKRHPMSTDMNDADLKRLDNFTDPEQKLLQRDAPSVEGSEA
                     SKPSNGHKNT"
     gene            complement(1196..1405)
                     /locus_tag="VFH39_03365"
     CDS             complement(1196..1405)
                     /locus_tag="VFH39_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924840.1"
                     /translation="MAEKNRRQPKGKAQSRARATAHKYDMKKMATVVNHVDYERRHGY
                     ESAHHREKKAGRRPESPDQQPPQAA"
     gene            complement(1430..1792)
                     /locus_tag="VFH39_03370"
     CDS             complement(1430..1792)
                     /locus_tag="VFH39_03370"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HET6924841.1"
                     /translation="MSYLELIRRIKDMAHVDEDESQDALELVVENVAAHLTDHTRREF
                     AAALPDELQSAAQMVPTANHIDEDIIEQLMDLEDVDEVGAKQRIRAAWQAICDIFDSR
                     EVDDITSELPRNVVSALQ"
     gene            complement(1854..>2269)
                     /locus_tag="VFH39_03375"
     CDS             complement(1854..>2269)
                     /locus_tag="VFH39_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6924842.1"
                     /translation="ITRLTGIEPHETAGQPLFGDMTSELSRLFDGAVFVAHNVGFDYG
                     FFQAEFARLGNRFAMDKLCTVRLSRALFPHERTHKLDAVIARHGYRVANRHRAYDDAE
                     VLHRFYHEQLERLGAAVFYPVAERLLQPTATRQFM"
BASE COUNT          440 a          682 c          647 g          500 t
ORIGIN      
        1 gggcccattc aaccagcagg gcgaccccga aaccgatctg cgccactatg cccagccaca
       61 aaaagacgat gaacggctgc tcgacctcgt tcggccgggc aggcgcgcta taggtcttag
      121 cctgccagaa ccagctcaat agcttgaacg gcgagtagcc gaaagtccgc agccgcgtca
      181 cgagcgccgc cggcagaccg ggaccgtaca agctggcgat tccgcgtagc cacatatgct
      241 aggtattgtt tcatttttga tagcatgacg caattcggta tgccggaggc ctttttacat
      301 gtttctcgcc tgcgagcagc ggtacactat atgggtatgc cagcgctcga cccgaccacc
      361 cgacatgcta tcagccatac gctgcactgc ctcacgggct gcggcatagg cgaggtgctg
      421 ggcagtgcca tcggctcggc cgccggctgg cccaatctgt ggcagaccgt gctggcagta
      481 gtacttgcgt ttgcgttcgg ctacggcctg acctaccgtg gcgcacggcg ccatggcgct
      541 tcagccgccg atgcccgtgg caccgcgctg cgcaccgaca cggtctccat catcagcatg
      601 gaaatcatcg acaacgtact cgaatacctc atacccggcg ccatgagcgc cgtggtcgcc
      661 agctggctgt tctggtggag cttggcccta tcgctggggg tagccttcgt agtaaccgta
      721 ccggtcaacc ggctgatgat gagccgcttc ggcatcggcc atggccatgg cgcccaccac
      781 tagaaatatc cgctttggtt tgctatactt gcccctgttc ttacgcgggg ctgtagctca
      841 gttgactaga gcgcttccat ggcatggaag aggtccaggg tttgagtccc tgtagctcca
      901 ccacgtaaaa tactctggcg aaagtcagag tattttacgt tatacatatg accttaaatg
      961 gcgaagttaa atatcgcact gcccttgaat gcggcttccg gcacactact gcggtaacgc
     1021 ggaggaacag taccatggca aaagataagc gtcaccctat gagtaccgat atgaatgatg
     1081 ccgacctgaa gcggctcgat aacttcaccg accccgagca aaagctgctg cagcgcgatg
     1141 caccgagcgt agaaggttcc gaggcctcta agcccagcaa tgggcataag aacacttagg
     1201 cggcctgggg cggttgctgg tcggggcttt ctgggcgtcg gccggctttc ttttcgcggt
     1261 ggtgtgcgct ttcgtagccg tgtcgccgtt cgtagtccac atggttcacg accgtggcca
     1321 tctttttcat atcgtactta tgtgccgtcg cccgggcacg gctctgcgcc ttccctttgg
     1381 gctgacgacg gttcttttct gccatagaca tatggttctc ccttcaattt tactgtagtg
     1441 ccgagacgac gttgcgcggc aattccgagg tgatgtcgtc gacctcccgg ctgtcgaaga
     1501 tatcgcagat ggcttgccag gccgcgcgta tgcgctgctt ggctccaact tcgtcgacgt
     1561 cctccaggtc catcagttgc tcgatgatat cctcatcgat atggttggcc gtcggcacca
     1621 tctgggcggc actctgcagc tcgtccggca gtgcggccgc gaactcgcgg cgcgtatggt
     1681 cggtcaggtg ggcggcaaca ttctccacga ccagctccag ggcatcttgc gattcgtcct
     1741 catcgacgtg cgccatgtcc ttgatgcggc ggatcagttc taaataactc atatgcattc
     1801 tccttccgac tcataatatg cagtccggag ctggaagatt gctataagct tttttacata
     1861 aattgccggg tagcggtagg ctgcagcaaa cgttcagcta cggggtaaaa aactgccgcc
     1921 cccagccgct ctaattgttc gtgataaaac cggtgcagca cctctgcatc gtcatacgcg
     1981 cggtggcggt tggccacccg gtagccgtgg cgggcgatga ccgcgtctag cttgtgggtc
     2041 cgctcgtgcg ggaacagcgc tcggctgaga cgcaccgtgc agagcttgtc catggcaaag
     2101 cggttgccta agcgcgcgaa ttccgcctgg aagaaaccgt agtcgaaccc cacgttatgc
     2161 gccacgaaca ccgctccgtc gaacagacgg ctcagctcgc tcgtcatatc accgaacaag
     2221 ggctgcccag ccgtttcgtg cggttctatg ccggtcagcc gggtaatcc
//
LOCUS       DASSFD010000143         5665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1581965, whole genome shotgun sequence.
ACCESSION   DASSFD010000143 DASSFD010000000
VERSION     DASSFD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581965"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..617
                     /locus_tag="VFH39_03380"
     CDS             222..617
                     /locus_tag="VFH39_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924843.1"
                     /translation="MNVASLELCKELYELSGWSLATSKWYESWGDSDWSIADKYNPNC
                     PENYPAYDLGYLLRKLPWMIGAPRALLYMQKREKGYYYAYTTSTGEDWPFDERATVFK
                     VEDTPEDSAAMLAIELFKQGVLSKSPTVR"
     gene            829..2217
                     /locus_tag="VFH39_03385"
     CDS             829..2217
                     /locus_tag="VFH39_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET6924844.1"
                     /translation="MGRRWISYSRVSDDRSGRGRSVEEQDAENHRTAERLGDEIVASL
                     VDNSIGASRYSKGKRVEWQHALKMLERREADGVMTWEASRATRDLDVYVQLRKVCREA
                     GALWCYNGTVYNLNDGEDSFRTGLDILLAENEVDKTRKRVMRSTQANAERGLPHGKLL
                     TGYKRVYSGNGVFVEQVKDPAWQPLIEEAFRRKLAGELDAHIVRDFQRRGLKTLRGAD
                     WSMSQIKRLLMNPGYAGLRVHRGNIIGPAAWAEHAYITEAEHYQIVAGYNARTAAKRH
                     DGSIKYLLSGAARCGVCGGPVRVGSPRGYKSYHCDTGFHVSRKVNALDDLITELVLAR
                     LERPDILAALAVDNEPALQAIDRLSGLRMRLDGFYQAAAAGDLTPQALASIEKNLLPQ
                     IEQAEAEARPKNVPSVVTDLVASPRTIWPQLTIVQKRQVIALLMTVYIDRTRRGERVF
                     NPDSIRISWKVG"
     gene            complement(2303..2536)
                     /locus_tag="VFH39_03390"
     CDS             complement(2303..2536)
                     /locus_tag="VFH39_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924845.1"
                     /translation="MEHHIYPGQDLEGALELIDDPVERAHLIDEAYANIGHVALEPVV
                     VEDDYDQDGYYIGSAMLPDEQLGMGLSGETTVL"
     gene            complement(3689..3913)
                     /locus_tag="VFH39_03395"
     CDS             complement(3689..3913)
                     /locus_tag="VFH39_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924846.1"
                     /translation="MNPAALSRIQAAERRANQRDFAIRYGTHKSHPYGYVPQKRRTAR
                     YSATGLLIENLVVVGCLVILAAVIALTATK"
     gene            complement(3913..4125)
                     /locus_tag="VFH39_03400"
     CDS             complement(3913..4125)
                     /locus_tag="VFH39_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924847.1"
                     /translation="MSMTRYGQAAQYYSYYVSARPFSRFTQDAAEIAANERWSADVEA
                     WARLYLDEYGFLSQAGREQYWPKARA"
     gene            complement(4202..4906)
                     /locus_tag="VFH39_03405"
     CDS             complement(4202..4906)
                     /locus_tag="VFH39_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HET6924848.1"
                     /translation="MQLTGRDLTICTLLFRFGQLTSAQIASLVFSELSSHTPAARTLA
                     RLTDNGFLHRIERRIVGGARGGSGVYVYCLGVRGHKLFSEARYTPTRTVQHHSLAIAD
                     CYVMLRRLERAGRLSIRGYATEPDCHVRIDRYELKPDLYVELDRPAVDPIRFMYEIDM
                     GTQGQRQITEKFVRYWGAYNAASEAEWPPNQFVLFVAVDEQRAQELRWLLEKGDPAQQ
                     AIFRIKTAGQLEAELS"
     gene            5363..5584
                     /locus_tag="VFH39_03410"
     CDS             5363..5584
                     /locus_tag="VFH39_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924849.1"
                     /translation="MQQDLLRNHANGEGTKPILSVRLPEPYKQALEQLAQKRETSAAR
                     VLLAAGLAGDPELRQLTKQYSKAASSTRS"
BASE COUNT         1251 a         1683 c         1615 g         1116 t
ORIGIN      
        1 ctgggcgacg agtggacgga gttcggcgac ggcaacggcc ccatcgccat catccgcacc
       61 gacaccatcg cccgcattga ccgactcggg tttaagacct cgtaggcagc aaatagtaaa
      121 accgcccaga gcgaggcaac gctaaaggcg gttttactat tgcattttac aagcaaatgg
      181 tttatatatg aaagcacaat taaatagcga ggcaacgcaa tatgaatgta gcatcacttg
      241 aactgtgcaa agagttgtac gaactcagcg ggtggagctt ggcaacaagt aagtggtacg
      301 aaagctgggg tgatagcgat tggtctatcg ctgacaaata caacccaaac tgccctgaga
      361 attatccagc ctacgacctg ggctatctac tacgaaagct accgtggatg attggtgcgc
      421 cgcgcgcgct tctttatatg caaaagcgcg aaaaaggcta ctactatgcc tacacaactt
      481 ccactggtga ggactggccg ttcgacgaac gagcaacagt attcaaggtc gaagacaccc
      541 ccgaagactc cgccgccatg ctagccatcg agctgttcaa gcagggcgtt ctcagcaaat
      601 ctccaactgt tcgatagacg ttgcctcggg tacctcggag gggtatgcaa accaccacaa
      661 acacccctgt cacgatgcct ccgcgtgtcg ctccaaagcc ccgacccagt gacgttgtaa
      721 cttgccctga ccgaagtaaa tgcgacgggc tgacaccaag aacgtgcaat ggagcctctt
      781 attgccaaat tgacagagac gtaggacaat tagaacggta agacgaatat gggaagacgc
      841 tggataagct attcccgcgt ctccgacgac cgcagcggca gaggccgcag tgtcgaggaa
      901 caggacgcgg agaaccatcg caccgctgag cggttaggcg atgagattgt cgcgtccctc
      961 gtcgacaaca gcatcggggc gagccgttac agcaagggca agcgcgtcga gtggcagcac
     1021 gccctcaaga tgctcgaacg ccgcgaggcc gacggcgtga tgacctggga agcctcccgc
     1081 gccacccgcg acctcgatgt ctatgtccag ctccgcaagg tctgccgcga ggcgggggcg
     1141 ctgtggtgct acaacggcac cgtctacaac ctcaacgacg gcgaagacag cttccgtacc
     1201 ggcctggaca tcctgctcgc cgagaacgag gtcgacaaga cccgtaagcg cgtcatgcgc
     1261 tcgacccagg ccaacgccga gcgcggccta ccccacggca agctgctgac cggctacaag
     1321 cgcgtctata gcggcaacgg cgtctttgtc gagcaggtca aagacccggc atggcagccg
     1381 cttatcgaag aagccttccg ccgcaagctg gccggcgagc tggacgccca catcgtccgt
     1441 gacttccaac ggcgcggcct caagaccctg cgcggcgctg actggtcgat gtcccagata
     1501 aagcgcctcc tgatgaaccc cggctacgcc ggcctgcgcg tccaccgggg caacatcatc
     1561 gggccggccg cgtgggccga gcacgcctac atcaccgagg ccgagcacta ccagattgtc
     1621 gccggctaca acgcccgtac ggcagccaag cggcacgacg gcagtatcaa gtacctgctg
     1681 tccggggcgg cccgctgtgg ggtctgcggt ggccctgtgc gcgtcggcag tcctcgtggt
     1741 tacaagagct atcactgtga cactggcttc catgtcagcc ggaaggtcaa cgcgctggac
     1801 gacctcatca ccgagctagt cctggcccgc ttagagcggc ctgacatcct cgccgcgctg
     1861 gcagtcgata acgaacccgc cttgcaggct atcgaccggc tgagcggcct cagaatgcgc
     1921 ctggacggct tctaccaggc ggcggctgcc ggtgacctga ccccgcaagc cctggccagc
     1981 atcgagaaga acctgctgcc gcagatagaa caggccgagg ccgaggcgcg tcccaagaac
     2041 gtgccgagcg tggtgactga cctggtagcc agcccgcgca ccatctggcc gcagctgacc
     2101 atcgtgcaga agcgccaggt gatcgccctg ctgatgacgg tctacatcga ccggacgcgc
     2161 cggggcgagc gcgtgttcaa cccggacagc atccgtatca gctggaaggt cggataagcc
     2221 ggcgcaaggc gcccagctgg tcttccgaca gctcgggtgc cgcttgtagc tgtttctgta
     2281 tccacttgtt catctgcatt gattataaaa cagtcgtttc gccgctcaaa cccatgccga
     2341 gctgttcatc cggcagcatg gccgagccga tgtagtagcc gtcctggtcg taatcgtcct
     2401 cgacgacgac cggctcaagg gcgacatggc cgatgttggc atacgcctcg tcgatgaggt
     2461 gcgcccgttc cacggggtcg tcaatcagct ctaaagcgcc ttcgaggtct tgtccggggt
     2521 agatgtggtg ttccatgcaa gtaggtatga acctaacggc acagaaagta aataggtttt
     2581 acaaggcttt acgcggtggc aatacaacca ggtgtatgcc gtcccattcc atctcgccac
     2641 gttcatacgc ggcttctctg tgtttcttac tcataagcgt ctcccttaca ggcgagactg
     2701 taggcggttg ccctcacgaa gtctgtaagc tggcttacac aaggcatggg gacgccttcc
     2761 ctctgcaatg aagctatggg cctaagcgtc tcttacatcc ccctggatgc ggtttacaag
     2821 tcatcgggtt ttaccggcag tttccgtccc tcacggatta ctccccatcg acgggtcgct
     2881 catgtgcgct gcaaaggagg ccaggggtga cgtagcaggc gtgctggact actcatctag
     2941 gtagtcacta cgctctatcg aggcggcacc aagtgtttcc tacatcctcg ttaccgagag
     3001 ttccctttga gctattgcta gtcgccggtc gtttagtgtc agcaccgtca tttatagagc
     3061 tatcatagcc ctggactaac gcaccgaaca cgagctgttg taggcagaag ccaggagagc
     3121 tgatcgtaca gccggagggt gagaattaac taccctcttt atactcctag cctcgcggct
     3181 tcggactctc ctgattgccg cctacaacgg tcgctctttg ttgacaaaac tggagcgata
     3241 gacagttcga cttgtaaatg tatgtgccag tgtgctttaa tgataagcga acaacagcag
     3301 tgcgccccgc aaggggcgtt tttctgttgt aactgacgac ctcgtgcttt ttggattgat
     3361 tgaacaacgc agttagtcgc tcctatgtaa ccatgagcgc cgtgcaaaat caatattgct
     3421 tttctgctgc gcttgtcata taaggtaagc ataaccgcat tgtgccggct tttgcgcctc
     3481 agccaagcga ctcgcacctt accgtaaact ttcataggaa tcggtgcgag gttttccgtt
     3541 tctttcctcg cccggagtct cacttactcg ttgccagcta catcccaccc gtagccggca
     3601 ccacgcctcg gcagtggatg gacatgaccc atcagaacgc cgaccagcat tagaacagcc
     3661 ccattctctg agggctgttc gtttgttatc acttggtcgc tgtgagcgca atcacagccg
     3721 ccagaattac caggcagccg accacgacca gattctcgat aagcaggccg gtggcgctgt
     3781 agcgggctgt acggcgcttc tggggcacat agccgtaggg atggctcttg tgcgtgccgt
     3841 agcggatagc gaagtcacgc tgattggcgc ggcgctcggc ggcttggatg cgggataggg
     3901 cggcggggtt cattacgccc tcgccttcgg ccaatactgc tcacggccgg cctggcttag
     3961 gaaaccgtac tcatccaagt acagacgcgc ccaggcttcg acatctgcgc tccaccgctc
     4021 gttggctgca atctcagcgg cgtcctgcgt aaaccgactg aagggtcgag cgcttacgta
     4081 gtaggagtaa tactgtgccg cttgtccata tcgcgtcatg ctcatagcta tggttccttg
     4141 tttagttgat actcacagta tgcgctattc gtattacaaa tgcaagggtt tatgcacagc
     4201 tttaggataa ttcggcctct agctgcccgg ccgtcttgat gcggaatatc gcctgctgcg
     4261 ccgggtcgcc cttttcgagc agccagcgca attcctgcgc ccgctgctca tcgaccgcca
     4321 cgaacagcac gaactggtta ggcggccact cggcctcact ggcggcgtta tacgcgcccc
     4381 agtagcgcac gaacttctcg gttatctgcc gctgcccttg cgtccccata tctatctcgt
     4441 acataaagcg gatagggtcg actgctggcc tatcgagttc gacatataaa tccggcttca
     4501 gctcgtagcg gtcgatacgc acatggcaat ccggctcggt ggcatagccc cggatactca
     4561 gccgcccggc ccgttccaag cgccgcagca tgacgtagca atcggcaatc gctaatgaat
     4621 ggtgctgcac cgtccgggtc ggcgtatagc gggcctcgct gaacagctta tggcccctga
     4681 ccccgaggca atagacatat acgccggaac cgccgcgagc accaccgact atccggcgct
     4741 caatccggtg cagaaagcca ttgtcagtca ggcgggcgag ggtgcgggcg gcgggcgtat
     4801 gcgagctaag ctcgctgaat acgagtgagg caatctgtgc agacgtgagc tgaccgaagc
     4861 ggaatagcaa ggtgcagatg gtgaggtcgc ggccggtgag ctgcatgagc cgtattttac
     4921 gggagtttta cgagcggctg aaatgcgggt gcgaaatgcg cctgacgggc ggccgtgacc
     4981 ggcctcgctt ccggcggccg taaacggccc tgtgggggcc gtgcgcggca agtcgggggt
     5041 gggtttcgtg atgaagaacg aacgaacaca ccgccagcgg cgcgttacag ggtgcgaagc
     5101 ccgtggatgc tgggctggag cggtctgatc gagcgggcga actagcctcg aactatatag
     5161 ctacgacaca cttatgcaca ggcataacag gtatgacgca gtggtatgac aaatagtgct
     5221 tgcaattcat tagcaattcg tcatactaat gcacagcacc aaagcgagca ccaaacgtat
     5281 gacggatgcc gacctgctgg gcgctaccaa attatcaacc aacaaatcaa ctaaacaagg
     5341 agccatatgg aaaggacact aaatgcagca agacctgcta cgcaaccacg ccaacgggga
     5401 gggaacgaag ccgatactga gcgttcgact accggaaccg tacaaacaag ctctcgaaca
     5461 gctcgcccaa aagcgggaaa cgtcagccgc acgggtacta ctcgcagccg gtctggccgg
     5521 cgaccccgaa ctccggcagc tgaccaaaca gtacagcaaa gcggcctcgt caacccgttc
     5581 gtgaccaaac tgcttatcgg ggcgtgcatc acgctactgg tgcaggtagt cacgcacaag
     5641 tggaaggcga ccgccctaat ggctc
//
LOCUS       DASSFD010000144         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_412656, whole genome shotgun sequence.
ACCESSION   DASSFD010000144 DASSFD010000000
VERSION     DASSFD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412656"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFH39_03415"
     CDS             <1..458
                     /locus_tag="VFH39_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924850.1"
                     /translation="DVRRLRRELENLAESLEQLRLRTKAPATKLPRASRLLEEFAATN
                     RLNLLLPEHRELAAAYCASVLEHAPVLRMSFAAEPSRTFTTRLIVWLRSNLTDDVLLE
                     IGLEPTIAAGCILRTPRKQYDFSLRARFEAQAGLLAQKLHAVEESAHVG"
     gene            448..1875
                     /locus_tag="VFH39_03420"
     CDS             448..1875
                     /locus_tag="VFH39_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HET6924851.1"
                     /translation="MSDNRHFDHLVDKGNPVGEVVGVDRFLVRVRGMQPVNSRALVMF
                     EDGSKGFVYQIAEDHVVVLHLGDSQVRNGMAVVVQHHDLVSKVGKDFIGRVVSVTGEP
                     LDGKGPIAADAVWPVFHDAPALYEREMLDTQLATGVTVLDTMFPIVRGQRMAVLGDSK
                     SGKTALATQLVINQRTTDVVAIYVLIAKRRADIDQLLQRLQSSDAMANCIVIVATAFE
                     SLVLSYLAPYVGCAMGEYLWQQCDQDTLIVYDDLTTHAQVYREISLLAQTSPGRESYP
                     GDMFYAHSSLLERAGKLSRNHKTLTALPLVLASSGDITAFMPTNIMSITDGQWVLDMD
                     VFRDRMRPAVNIGLSVTRVGGRGQIERQKQQAVATVQALNAFAEAEQYAHFGSELALE
                     GQKDIAKGKRLYELMNQAPTETYTLMAQTLMLDIVLNLGDNQVLNMKALKDLAPGFAG
                     QVADDGAYDKVRKDLLEQSLLELKR"
     gene            1872..2723
                     /locus_tag="VFH39_03425"
     CDS             1872..2723
                     /locus_tag="VFH39_03425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6924852.1"
                     /translation="MRRPLEVAQERDAMGVIVNLTEAFEGLASMRISQIKNQVLESQR
                     FFEALWGIYNQLRIDPIFRFGRTAEEAEEAKELLIGITAEGGFSGDIDQQLISFMLKG
                     YDPEKQDIIIIGHHGAVQLAQAGVGFKKYFKLPSKEQNINVAPLVEQIRRYRSASVYY
                     QTYVSLMVQDVKRIELSKAVQEAGRKSAKSEEVISEDTYIFEPSSYDVIAHLERSMLY
                     ITMSQLILDSKLAQYASRFRAMNVANERADSSFKELRLLYNRSRRSEADERLKEILNG
                     QKKARSV"
     gene            2720..>2959
                     /locus_tag="VFH39_03430"
     CDS             2720..>2959
                     /locus_tag="VFH39_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924853.1"
                     /translation="MSPGVVRSVKNLVVRVEFDEAMPAVNELLEVAVGDTAVQLLVSS
                     LQPGIAVCLNIQADSRLQKGMTVTPTGRSVEVPVGD"
BASE COUNT          649 a          842 c          898 g          570 t
ORIGIN      
        1 gcgacgtccg tcgtctgcgc cgcgagctgg aaaacctggc agagagcctg gagcagcttc
       61 gtttgcgcac caaggcaccg gcgacaaaac tgccccgggc cagccgactc ctcgaggaat
      121 tcgcggctac caaccgcctg aacctcctac tgccggaaca ccgcgagctg gcagccgcct
      181 actgcgcttc cgtgttggag catgcgccgg tactgcgcat gagctttgcg gccgagccat
      241 cacgcacatt cacgacccgg ctgattgtgt ggcttcgcag caatcttacc gacgatgtac
      301 tgctggaaat cggtctcgag ccgaccatag cggccggctg catactgcgt acgccgcgta
      361 agcaatatga tttcagcctg cgggcccgct tcgaagccca ggctggatta ctcgcccaga
      421 aactgcatgc cgttgaggag tctgctcatg tcggataacc gtcacttcga ccatctggta
      481 gacaagggca atcccgtcgg agaggtggtg ggtgtcgacc gtttcctggt gcgcgtgcgc
      541 ggcatgcagc ctgtgaacag ccgtgcactt gtaatgttcg aagatggcag caaaggcttc
      601 gtataccaga tcgccgaaga ccatgtggtg gtactgcatc taggtgatag tcaggtgcgt
      661 aacgggatgg cggtggtggt gcagcatcac gacctagtat ccaaagttgg caaggatttc
      721 atcggccgcg tcgtatcggt aaccggcgag ccgctcgatg gcaaagggcc tatcgctgcc
      781 gatgccgtgt ggcctgtgtt ccacgacgcg ccggcactgt atgagcgtga gatgctcgac
      841 acccaactgg cgaccggcgt gacggtgctc gataccatgt tcccgatagt gcgcggccag
      901 cgcatggccg tactgggaga cagcaaatct ggcaagacgg ctctggcgac ccagctggtt
      961 attaaccagc gcaccactga tgtggtggct atctatgtgc ttatagccaa gcggcgggcc
     1021 gatatcgacc agctgctgca gcgcctgcag tccagcgatg ccatggcgaa ttgtatcgtt
     1081 atcgtagcta cggcgttcga atcgctggtg ctgagctatc tggcgccgta tgtcggttgt
     1141 gccatgggag agtacctgtg gcagcagtgc gatcaggata cgcttatcgt ctacgacgac
     1201 ctgaccacgc acgcccaggt gtaccgcgaa atctcgctgc tggcgcagac cagcccgggc
     1261 cgcgaaagct accccggcga catgttctat gcacactcat ccttgctgga acgggcgggc
     1321 aagctgagtc gcaaccacaa gacattgacc gcgttgccgc tggtgctggc cagcagtggt
     1381 gacataaccg ccttcatgcc gacgaacatc atgtctatca ccgatgggca gtgggtgctc
     1441 gacatggacg tgttccgcga ccgtatgcgg ccggcggtca atatcggcct gagcgtgacc
     1501 cgcgtgggcg gccgcgggca gatagagcgc cagaaacaac aggcggtggc taccgttcag
     1561 gcgctgaacg cattcgccga agcggagcag tacgcgcatt tcggctctga attggcgctg
     1621 gaaggccaga aggatatcgc gaagggcaag cgcctctacg agcttatgaa ccaggcgccc
     1681 accgaaacct acaccctcat ggcccagaca ctgatgctcg acatagtttt gaacctgggt
     1741 gacaaccagg tactgaacat gaaggccctc aaagacttgg cccccggttt cgccggccag
     1801 gtagctgatg acggcgcgta cgataaggtg cgtaaagacc tgctcgagca gtcgttattg
     1861 gagctgaagc gatgaggcgg ccgctggagg tcgcccagga gcgcgatgcc atgggtgtaa
     1921 tagtcaacct taccgaggcg ttcgaaggtt tggccagtat gcgtatctcc cagattaaga
     1981 accaggtact cgaatcgcag cgtttcttcg aagcgctctg gggcatctac aaccagctgc
     2041 gtatcgaccc catcttccgt ttcggccgca ctgcagagga agccgaagag gcgaaagaac
     2101 tgctcattgg tataacggcc gaaggaggat tcagcggcga tatcgaccag cagcttatca
     2161 gttttatgct caaaggctat gatcccgaga agcaggatat tatcatcatc ggccaccacg
     2221 gtgccgtgca gttggcgcag gccggcgtgg ggttcaagaa gtactttaaa ctgccctcaa
     2281 aagaacagaa catcaacgtc gcacctctgg tggaacagat ccgccgctac cgctcggcca
     2341 gtgtctacta ccaaacctac gtgtcgttga tggtacagga tgtgaagcgc atcgaactca
     2401 gtaaggcagt gcaggaagcc ggccgtaaat cggcgaaaag cgaagaagtg atcagcgaag
     2461 atacgtacat attcgaaccg agcagctacg acgtaatcgc ccacctggaa cgctccatgc
     2521 tgtatatcac catgagccag ctcatccttg atagtaagct tgcccagtac gccagccgtt
     2581 tccgtgccat gaacgtcgcc aatgaacgcg ctgattcgtc gttcaaggaa ctccgactgc
     2641 tgtataaccg tagccgccgg tccgaggccg acgaacgcct caaggagata ctgaacgggc
     2701 agaagaaagc gaggtcggta tgagccccgg tgtcgtgcgt tccgtaaaga acctggtggt
     2761 acgcgtcgaa ttcgacgaag ccatgccggc cgtgaacgag ttgctcgagg tggcggtagg
     2821 cgacacggcc gtgcagctgc tggtaagcag tttgcagccg ggcatcgcgg tatgccttaa
     2881 tatccaggcc gattcccgtt tgcaaaaagg catgacggtg acgccgaccg gccgcagcgt
     2941 ggaggtaccg gtgggcgat
//
LOCUS       DASSFD010000145         4612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_681892, whole genome shotgun sequence.
ACCESSION   DASSFD010000145 DASSFD010000000
VERSION     DASSFD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681892"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFH39_03435"
     CDS             <1..746
                     /locus_tag="VFH39_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6924854.1"
                     /translation="YMVQDVCYFLEKLGVKIEGIGSSTLRIRGVKDIKKNVTYAPAED
                     PIEAMFFTSAAVTTNSEITIRRVPIDFMSLELLKLKKMGLNFDESDRYKATNGRTDLV
                     DLTIHKHDGGLRALTEKIHANVYPGLNQDNLPYFVPIAAVAKGRTLIHDWTYENRAIY
                     YTELTKIGANVELADPHRLYVTGPTKFSKADVVCPPALRPASLLLIGMLAADGISMLR
                     NVYTINRGYEDLAERLNSLGAHITVISDL"
     gene            complement(749..2116)
                     /locus_tag="VFH39_03440"
     CDS             complement(749..2116)
                     /locus_tag="VFH39_03440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6924855.1"
                     /translation="MFNSWLLYLVAFANVMSMTSLGLYIIGANVYDIKKFRTRKLGAQ
                     GKRDNLPLVSVVVPAHNESVVIRRTLDSIRASSYPNIEIIVVDDGSTDDTAAIVREYI
                     MNQPKTVVSSYLSRSTGRSGQLVRRFLRCERDVTPIKLVWQANHGKAVAMNNAIENHV
                     HGSLLMCVDADSILHPKAIQQAVHYFKDPQIIGVAANVRVLDNGTLLGRLQRFEHLVG
                     YRSKKFYTLTNTEFIVGGVASTYRTDVVREVGGYDTDTVTEDIGLSMKLIATYGNRQC
                     RIVYAADVVAMTEGVQTYQQLMRQRYRWKMGSLQNLIKYGNLVANDNATKYSFLLSFY
                     RLPMAFLSELMLILQPLLLGYLIYLSVHYRNPYILFGAYFTITMYVLLTLWPDEYLTV
                     REKLKLSLQTLEIYILFYAMDVVQVSAIFRCLFNREQILKRNASSTWVSPTRAGQATP
                     TPVRV"
     gene            complement(2109..2678)
                     /locus_tag="VFH39_03445"
     CDS             complement(2109..2678)
                     /locus_tag="VFH39_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET6924856.1"
                     /translation="MRTIKFQAIVGCLLVAVGLGLFWLRDNNAFSHAQPAAPVANSTW
                     QAPKAAISGNPVRLQIPSLGMDLPIINGTYSKTTRTWTLTTNKVQYAVNTALPNDTTG
                     DTFLYGHYRPEVFARLHLIKPGATVTVTTDNGHIFTYTYTGYKVIDPSDTSIFQYQGK
                     PELTIQTCTGLFFEHRQLFHFNYDGVKNV"
     gene            complement(2792..2968)
                     /locus_tag="VFH39_03450"
     CDS             complement(2792..2968)
                     /locus_tag="VFH39_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924857.1"
                     /translation="MYHKVLGAVAPTGLGAVVLPNTGGNVALTVAAVTSLAIGGAILL
                     STVIRAVANRAYKA"
     gene            3194..>4612
                     /locus_tag="VFH39_03455"
     CDS             3194..>4612
                     /locus_tag="VFH39_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6924858.1"
                     /translation="MRLITNFKAKLIASVLALGMIGSLIVPVVTHAAVVNPAPTAKVS
                     FAFDDSLASAYTKAAPTLAKYGLTGTSYAITGCVGMTKVPNTCHANTDLPYMTWAQLK
                     ALQTTYHWEIGSHTVDHPCLASNAKADPSDCQTNTLTKAQVDAELLNSKTALANHGIT
                     ATAFAPPYGDYSNMVMAQIAKYYTSMRGFKEQGTNGWPLDDYLLNNITVQQRLTPVAT
                     LESKIDQAISSNSWVILSFHDIAAKPSQNPDDYQYGTAELAQVAAYVQSKQAAGLIKN
                     VNTSQGLVTSTTNMLPNGTFNDGVGDGWSTDDPIRITKDTASNGSYPDYTNSIKLVSN
                     PNGKQTHLFSPAVSVNPNTTYLFKNFLNVQKISSGEVGFYVDEYDVNGSWISGQYLKR
                     ENSSFVEDMNFAYKPTSLAVAKASLQVVMAGTGITAYLDNSQMFALSTATPTQINLLP
                     NGNFNAGIADGWSTDAAGNIVAD"
BASE COUNT         1111 a         1301 c         1213 g          987 t
ORIGIN      
        1 actacatggt gcaggacgtg tgctacttcc tcgaaaagct cggggtgaag atcgaaggta
       61 tcggcagcag cacgctgcgc atccgcggag tcaaagacat aaagaagaac gttacctacg
      121 ccccggccga ggacccgatc gaagccatgt tcttcacatc ggctgccgtg acgactaatt
      181 ccgaaataac tatccgccgt gtgcctatcg acttcatgtc gctcgagctg ctgaagctca
      241 agaaaatggg gctgaatttc gacgaaagcg acaggtacaa ggccaccaat ggtcgtaccg
      301 atctggtcga cctgacgatc cacaagcatg acggcggtct gcgcgccctg accgagaaga
      361 tccatgccaa tgtgtatcct ggactgaacc aggacaactt accgtacttc gtgcctatcg
      421 ccgctgtggc caaaggccgg acgctcatcc atgactggac gtacgaaaac cgcgccattt
      481 actacaccga actgaccaag atcggcgcca atgtggaact ggccgatccg caccgcctgt
      541 acgtgaccgg cccgaccaaa ttcagcaagg ccgacgtcgt ctgcccgccg gcactccgcc
      601 ccgcttccct gctgcttata ggcatgctgg ccgccgacgg tatctccatg ctgcgcaacg
      661 tctacaccat caaccgcggc tacgaagatc tggctgagcg cctgaactca ctcggcgccc
      721 acatcaccgt catcagcgat ctgtaagctt acacccgtac cggtgttggc gtggcctggc
      781 cggcccgcgt gggcgaaacc caggtactgg aggcgttgcg cttcagtatc tgctcacggt
      841 tgaacagaca gcggaagata gcagatacct gcaccacgtc catggcgtaa aagaggatgt
      901 atatttccag tgtctgtagg ctcagcttca gcttttcgcg caccgtcagg tattcatccg
      961 gccaaagcgt cagcagcacg tacatagtga tggtgaaata cgccccgaac aggatgtacg
     1021 ggttacggta gtggacgctg aggtagatga ggtagccaag cagtagtggc tgcagaatga
     1081 gcatcagctc gcttaagaaa gccatcggca agcggtagaa cgacagtagg aagctgtact
     1141 tagtggcatt gtcattggct accagattgc catactttat gaggttctgc agactgccca
     1201 tcttccagcg gtagcgctgc cgcatcagtt gctggtacgt ctgtacgcct tcggtcatgg
     1261 caaccacatc ggccgcatac acaatacggc actggcggtt gccgtatgta gctataagct
     1321 tcatgctaag cccaatgtct tcggtgacgg tgtcggtatc atagccccca acttcccgaa
     1381 ccacatcggt gcgataagtc gaggcgacgc cgccaacgat aaactcggta ttggtgaggg
     1441 tatagaactt cttggaacgg tatccaacta agtgctcaaa gcgctgcaaa cgacccaaga
     1501 gagtgccgtt atccagcacc cggacattgg ctgcgacacc gatgatttgg ggatccttga
     1561 agtagtgcac cgcctgctgg atagccttgg ggtgcagaat agaatcagcg tctacgcaca
     1621 tcagcagcga gccgtggaca tgattttcta tggcgttgtt catcgccacg gccttaccgt
     1681 ggttcgcttg ccacaccagc ttgatcggcg tcacgtcgcg ttcgcaacgc aggaagcgac
     1741 gcaccagctg cccggaacgg cctgtgctgc gactcaggta tgaagacacg acggttttcg
     1801 gctggttcat aatgtactct cggactatgg cggctgtatc atcggtcgaa ccgtcatcga
     1861 ccacgataat ctcgatattg ggataactgc tggcgcggat agaatcgagg gtgcgccgga
     1921 tgacgaccga ctcgttgtgg gccggcacga ccacactgac aagcggaaga ttatctcgct
     1981 tgccctgggc gcccagcttg cgcgttcgga acttcttgat gtcatataca ttggcaccga
     2041 tgatgtacaa acctaagctg gtcatgctca tcacgttggc gaaggccacc agatagagca
     2101 gccacgaatt aaacattttt cactccgtcg tagttgaagt ggaacagttg gcggtgttca
     2161 aagaacagtc cggtgcaggt ctggatagtc agttccggtt tgccctggta ttggaatata
     2221 gacgtgtctg acggatcaat tactttgtag ccggtgtagg tgtaggtaaa gatatgaccg
     2281 ttatcggttg tgaccgtgac ggtcgcgcca ggcttaatga gatgcaggcg cgcgaacact
     2341 tccgggcgat agtggccata taggaacgta tcacccgtcg tatcattggg gagtgccgtg
     2401 ttaactgcat actgaacctt attggtcgtg agcgtccagg tgcgggtagt cttgctgtag
     2461 gtaccattga tgattggcaa atccatgcca aggcttggaa tctgcaggcg taccgggttg
     2521 ccactgatgg cggcctttgg tgcctgccag gtgctgttag ctaccggtgc tgccggttgg
     2581 gcatgcgaaa atgcgttatt gtcccggagc caaaataggc ctaagcctac ggccaccagc
     2641 aaacaaccca ctattgcttg aaacttaatc gtacgcatga atttcatgac aatcatctcc
     2701 accaattaaa aagagagggg cgagtcgtct agacgactgc gcaccctctc tttaatatgc
     2761 ggtcgatccc cacccgctgg ctactaaggc cttaggcctt gtaagcgcgg ttggccactg
     2821 cacggatgac agtagacaac aggatggctc caccgatagc caggctggtg acagctgcca
     2881 ccgtcagggc cacattcccg cccgtgtttg gcaacacgac ggcgcctaag ccggtgggag
     2941 cgacagcgcc aagaactttg tggtacatat cttcacctct taatttataa ccgtaacgta
     3001 ttttacacta tttgtgcatt tttgtaaatg gtgtagtgta atatcaccat tgcttacgct
     3061 taataactgc aggcgaaagg cctaatatac caagagaata ttgctttttt tgtaaattgt
     3121 agtataacta gtatcaagtg taacggaacg gtgactcgat gggtcgggca gagcacccca
     3181 gaaggtaagg tctatgcgtc ttattactaa cttcaaggcc aaactaatcg ccagcgtctt
     3241 ggcgcttgga atgatcggca gcctgatagt gccggttgtg acccatgctg ctgtcgtcaa
     3301 cccagccccg accgccaaag tcagttttgc cttcgacgat agcttggcaa gtgcctatac
     3361 gaaggccgca ccgaccctgg ccaagtacgg ccttaccggt accagttacg ccataaccgg
     3421 ctgtgttggc atgacgaaag tgccgaatac ttgccatgct aacactgacc tcccttatat
     3481 gacctgggcc cagctcaaag cccttcagac tacttatcac tgggagatcg gctcccatac
     3541 tgtcgaccac ccatgtctgg ccagcaacgc caaggccgac ccgtctgact gccagaccaa
     3601 cacgctcacc aaagctcagg tcgatgccga gctcctgaac agtaaaacgg ccctggctaa
     3661 tcacggcatt accgccactg ccttcgcacc accctacggg gactacagca acatggtcat
     3721 ggcccagatt gccaagtact acaccagtat gcgcggcttc aaggaacaag gtaccaacgg
     3781 ctggccgctg gacgactacc tgctaaacaa catcactgtg cagcagaggc tgacgccggt
     3841 tgctacgctc gaaagcaaaa tcgaccaggc tatttccagt aatagctggg tcattctttc
     3901 gttccacgac atcgccgcca agccgagcca aaatcctgat gactaccaat acggtaccgc
     3961 cgaactggct caggtggctg cctatgtaca gtcaaagcag gcagccggat tgataaagaa
     4021 cgtgaacacc agccaggggt tggtaaccag cacgacgaac atgctgccga acggcacatt
     4081 caacgatggc gtaggggatg gctggagcac cgatgatccc atccgcatta ccaaagacac
     4141 cgcttcgaat ggcagttacc cggactacac gaactccatc aagctggtct ccaacccgaa
     4201 cggcaagcaa acccatcttt tctcccccgc agtcagcgtc aatccgaaca ccacctacct
     4261 gttcaaaaac tttctgaacg tacagaaaat cagcagcggc gaagtcggtt tttacgtcga
     4321 cgaatacgac gtgaatggca gctggatctc cggccagtac ttaaagcgcg aaaactccag
     4381 tttcgtggag gacatgaact ttgcctacaa gcccacctcc ctggcggtcg ccaaggcaag
     4441 tttgcaggtc gtcatggccg gcaccggcat cactgcctac ctcgataaca gtcagatgtt
     4501 cgccctgagc accgctacac cgacccagat caacctgctg ccgaatggca atttcaacgc
     4561 cggcattgcg gatggttgga gcaccgatgc cgctggtaac atcgtggccg ac
//
LOCUS       DASSFD010000146         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1196857, whole genome shotgun sequence.
ACCESSION   DASSFD010000146 DASSFD010000000
VERSION     DASSFD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196857"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..512
                     /gene="ssrA"
                     /locus_tag="VFH39_03460"
     tmRNA           119..512
                     /gene="ssrA"
                     /locus_tag="VFH39_03460"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            796..1527
                     /locus_tag="VFH39_03465"
     CDS             796..1527
                     /locus_tag="VFH39_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924859.1"
                     /translation="MRIYRNKSGFTAVAALLIALVLAFIGLVGYYVWHGQKHRSNSTP
                     VTYQIIKTADGKIQLSLPSTWHVIADSNNPKADQIISVSQNTHRCHKDDPTNCSGEPC
                     LDAKDPAPCIYEAEFQPKALDPQQDNKWLLTVEKTAMTITQATSQLRDTLAASTVLPS
                     GHTVQKSTDKINGYDAVYVETKGGDCSDWCYTSIDYLIESNGYLVHLSNNEQFVSQSA
                     NPVSQDYSAYAPDFSKIVHSIKLNF"
     gene            1840..2247
                     /locus_tag="VFH39_03470"
     CDS             1840..2247
                     /locus_tag="VFH39_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924860.1"
                     /translation="MASKAPPKKLTIRKKVAMTAAATSMLAVGTASMGVASADSYWSN
                     SNNYSNGYSSYNTYGSYGGYSSYNNYGNYWNNYRPTSMYSQYRWVYDPCLHRMVLIGY
                     STYYHRWQYCNSYDRYSMWSSPYRSYSYNNYWY"
     gene            2461..3018
                     /locus_tag="VFH39_03475"
     CDS             2461..3018
                     /locus_tag="VFH39_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924861.1"
                     /translation="MATSKNERRKLIDKALVTLGMVATLALLAIGGVAWWAYGFTSGN
                     VHDELAQQKIFFPEKGSAALNALPPAQQAAVSQYAGQQVLDGAQAKVFANDYIAVHLQ
                     KIGGGLTYSEVSAKAMTEPTNAKLQAQANTLFKGETLRGLLLGDAYAFWTIGQIAKVA
                     AIVAVVAGGVMLVLVVLGIRHLSGL"
     gene            complement(3026..4018)
                     /locus_tag="VFH39_03480"
     CDS             complement(3026..4018)
                     /locus_tag="VFH39_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET6924862.1"
                     /translation="MPYTLTVLVLVIVIATGIGAFLWRRFSIRGLLSRGDSLAGSPDD
                     SAIIAYYTAGNLLTAAGRSKLNGMKYSSYVVMPDGDGTYVDDVSAIIALDLPFNTSVH
                     LVGLSKQHRIDRVAFADFLRANGMEAVELEGDFPDYFELYAAKGQQVSARQIMNPRSM
                     QFAIDYCRSHFWEVNASELYVVATGHDKQHDSIIDDSQRFVSLLRPALLPGQPGAAPV
                     HHEVPYGEYDGPPLPCPICRQAMRITDNWQRCPAGHGILLDGKDLVRLRHGRLKIDND
                     PKSAAPHEQLICPNCHNPMVHVDYQDSGVVIDSCEHCPFRWLDADEVTAIEARE"
     gene            complement(4085..>4471)
                     /locus_tag="VFH39_03485"
     CDS             complement(4085..>4471)
                     /locus_tag="VFH39_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6924863.1"
                     /translation="LDFLARTRLLNYMLPELAVQVDFDQDSPYHELTLWEHSVKTVRL
                     SPAEVNVRWAALLHDSGKPYTRTVNKHGYSNYVHHEAVGAELATKVGHYLHWSKQRIQ
                     EVHDLVLHHLEPGSPIGDADGQATKR"
BASE COUNT         1066 a         1166 c         1266 g          973 t
ORIGIN      
        1 agctgaccaa atcagggaga gaagtcgacc gtttgggcag ctgagaggag gaccgtagcc
       61 gtaaatcgaa gctttcgccg tttcggcgaa acgagatcaa ggcgtacggg acgacgaagg
      121 ggctgaattt taagctcgac gttggacttt atcggttaga tcagcaggtc gcttgtcagc
      181 gtatagacac ctatgaatag atgcaaattc tactcgtaag caactcgttg ccgaagtagc
      241 aaacttgttt gctcctaagg ccgattttgc gctcgcagcc taattagtta gactgcaatg
      301 cctgacgcga tttcagatag ctgaatggcg tttcatattt tctggatgct caattgaatc
      361 catccgactc agttgctaag accaggattg ctcgctcgga atgtttgtcc gtttatatac
      421 ccgagcgcta agaccctaaa acgaactatg cctgtagacg actaaccaag ataaaccggc
      481 ggacgcgggg gcagtacccg ccagctccac caatgcaagg acccgaccct aaaggtcgga
      541 tttttgcatt ggtggcaaca cttgaggcgg gtactgcccg ggagggtgag gactgccggt
      601 ggcagtccga ggagtccttg cttgaccgag cgagcgtggc ggctgaagcc gacacgttgc
      661 cgagcgagta gagaaatgcg tacccgccag ctccaccata agcatctgac ctctgctaaa
      721 tggaggtctt tttgttttca aatacatatt agcttatgct tattacaata caagtaacta
      781 caggctataa ccagaatgcg catataccga aacaagtcag gctttaccgc cgtagcagca
      841 ttactaatcg ccttagtttt agcattcatt ggtcttgtcg gttattacgt gtggcatggg
      901 caaaaacaca gaagcaattc gacgccagtg acgtatcaga taatcaagac agcggacgga
      961 aagatccaat tatccctacc cagtacctgg catgttatcg cggatagtaa taacccaaaa
     1021 gcagaccaaa tcataagtgt tagccaaaat acgcataggt gccacaaaga tgacccaacc
     1081 aactgtagcg gggaaccatg tttagacgca aaagacccag cgccatgcat atatgaggcc
     1141 gaattccagc ccaaggctct tgaccctcag caggacaaca agtggttgct gacagtcgaa
     1201 aagaccgcta tgaccataac tcaggcaacc agccagttgc gagacacctt agcagccagt
     1261 actgtattgc cgtcgggcca tacagtgcag aagagtacgg acaagataaa tggctatgac
     1321 gctgtctatg tggaaacaaa ggggggtgac tgctcggact ggtgctatac gagtattgac
     1381 taccttattg aaagtaacgg ctacctggtc catctaagta ataatgagca gtttgtcagt
     1441 caatcggcca atcctgtcag ccaggactac tcagcctatg cacccgattt ttcaaaaatc
     1501 gtgcacagca ttaagctcaa cttctaatat attgggcgat agcctctcgc gggtaccctc
     1561 cctcacgttt cttttgccct gctaggtagc tatgacgatc gcccagcgca aatccttcgc
     1621 ccggatatct cttttattat gaagggcgat ttttcgggtt aatccgccaa taggaggtct
     1681 gagtgaaagc tcgggctttt tcatttgcag gggctatgtg attcattgct gtgaggcgga
     1741 ttacggcggg gtgttcggaa tttgtttagg ctaactgtag ataagctgag aaccaggcag
     1801 cgtccgagcg ggaagtaatt aagtaaggga gggatgcgta tggcaagcaa ggctccacct
     1861 aaaaaactaa caatccgcaa aaaggtcgcg atgaccgcgg cagccaccag tatgctggcc
     1921 gtcggcacgg cctcgatggg cgtagcgtcg gctgactcat attggtcgaa tagcaacaac
     1981 tatagcaatg gctatagcag ctataacacc tatggaagct atggcggtta cagcagctac
     2041 aacaactacg gcaattactg gaacaactac cggccgacga gcatgtacag ccagtaccgt
     2101 tgggtgtacg acccgtgcct gcatcgtatg gtactgattg gctacagcac gtactaccac
     2161 cgctggcagt actgcaatag ctacgaccgc tactcgatgt ggtcgagccc gtatcgcagc
     2221 tacagctaca acaactactg gtactagaca gcgacagagt gacagcgttt agggcgctcc
     2281 cgcgtaagca gggagcgctt tttatgaatg gttgaggtag gtttcggctc atatatcggc
     2341 atacatctga agggtagaga gaaaaacgtt gtagcggaac gcttgacctg tgtcacaagt
     2401 aggcagccag ggagttgcta tactttcagc agacagacaa cataaacagg aagacaaccc
     2461 atggcgacaa gcaaaaacga acggcgcaag ctgatagaca aggctctggt gactttgggc
     2521 atggttgcca cactggcgct gttggcgatc ggcggggtgg cctggtgggc ctacggcttt
     2581 acgagcggca acgtgcatga tgagctggcc cagcagaaga tattctttcc cgaaaagggc
     2641 agtgcggcgc tgaatgcctt gccgccagcc caacaggcgg cggttagcca gtacgccggt
     2701 caacaagtgc tggacggagc gcaggccaaa gtgttcgcca acgattacat cgccgttcat
     2761 ctgcagaaga tcggcggcgg tctgacgtat tccgaggtca gcgcaaaggc catgactgaa
     2821 ccaacaaacg cgaagctgca agcccaggcc aatacgctgt tcaagggcga gacgctgcgc
     2881 gggttattgc tcggtgatgc ttacgccttc tggaccatcg gccagatcgc caaggtcgcg
     2941 gccatagtgg ccgtggtcgc cggcggtgtc atgctggtat tggtcgtgct cggtatccgg
     3001 cacctatctg gcttgtaagc tggcgttact cccgggcttc gatagccgtg acttcgtcgg
     3061 catcgagcca gcggaacggg cagtgctcgc atgaatcgat gacgacgccg ctgtcctggt
     3121 agtccacgtg caccatcgga ttgtggcagt tagggcagat cagctgttca tgcggtgctg
     3181 ctgacttggg gtcgttatcg attttcagtc ggccgtgtcg tagccgcact aaatccttgc
     3241 cgtccagcaa gatgccatgt ccggccgggc accgctgcca gttgtcggta atgcgcatcg
     3301 cctgccggca aatcgggcaa gggagcggcg gaccgtcgta ctcaccgtaa ggtacctcat
     3361 ggtggacggg cgctgcaccc ggctgccccg gtagcagagc tggccggagt aggctgacaa
     3421 accgctgact gtcatcgatg atgctgtcat gctgcttgtc gtggccggtc gcaacgacgt
     3481 acagctcgct ggcattgacc tcccagaaat gcgagcggca gtagtcgatc gcgaactgca
     3541 tgcttcgggg gttcattatc tgccgggcgc tcacctgctg acctttggcg gcgtacagtt
     3601 cgaaataatc aggaaaatcg ccttccagct ccactgcttc catgccattg gcacgcagga
     3661 aatcggcgaa ggcgacgcga tcgatacggt gctgtttgct cagaccgacc aagtgcaccg
     3721 aggtgttgaa cggcagatca agcgcaatga tggccgagac atcgtcgaca taggtgccgt
     3781 cgccgtccgg catgacgacg taactcgaat acttcatgcc gttcagctta ctgcgcccgg
     3841 ccgccgtcag caggttaccg gcggtgtagt acgcgataat ggccgagtcg tcgggtgaac
     3901 ccgccaggct gtcgccgcgg ctcagcaggc cccggatgct gaaacgccgc cacaggaatg
     3961 cgccgatccc ggtggcgatt acaatgacca gtactagaac ggtgagtgtg tacggcatgc
     4021 tgtttgattt tcgatgactg ctcttaataa taacagacgt ctgagaggcc caagcggcgg
     4081 tcccttagcg cttggttgcc tgaccgtcgg cgtccccgat gggtgagccg ggctccagat
     4141 ggtgcaatac cagatcgtgt acttcctgta tacgctgctt tgaccagtgc aggtagtggc
     4201 ctaccttggt agccagctct gcaccaaccg cctcatgatg cacgtaattg ctgtagccgt
     4261 gcttgttgac cgtgcgggta tagggcttgc cgctgtcatg cagaagggcg gcccaccgta
     4321 cattgacctc cgccggcgac aaccggacgg ttttgacgct gtgctcccac agcgtcagtt
     4381 cgtggtaggg actgtcttgg tcgaagtcca cctggacggc cagttccggc agcatgtaat
     4441 tgagcagccg ggtgcgtgcc agaaagtcta a
//
LOCUS       DASSFD010000147         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1755639, whole genome shotgun sequence.
ACCESSION   DASSFD010000147 DASSFD010000000
VERSION     DASSFD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755639"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFH39_03490"
     CDS             complement(<1..356)
                     /locus_tag="VFH39_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924864.1"
                     /translation="MWLIPNQETVEALKASWWTWRPVVTLLRQASILDLERIGPIPES
                     SLYAEMTVEQAEQIATFLDSFLRRLEPNERVLLDGTLTDVPDTYEFYRAESELHKNYS
                     AEPEWLARFRDFCRVCG"
     gene            complement(481..1875)
                     /locus_tag="VFH39_03495"
     CDS             complement(481..1875)
                     /locus_tag="VFH39_03495"
                     /inference="COORDINATES: protein motif:HMM:NF033593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HET6924865.1"
                     /translation="MLRDRYDPVDVFQMVPQLSHAIEPVIAELDTLLDDDVILQQVRA
                     DLSQRYPHTLATGRPSTPVEVILRMLIIKHLYGWSYEQTEQWVSDSLVLRDFCRIYWQ
                     PVPDDTTLLRWANLIQPGTLQQLLDHVVEVACQLRVTRGRKLRTDGTVVETNVHQPTD
                     SSLISDGIRVISRILKSAQEVLISAGDAAQQLFRDRTRSAKRLNKQIIDTVRRRGEQA
                     EQVRKASYERLVEIGDALIAQAQAVGELLAVQHQRQADTLRNKLKQFMPRLQAVLTQT
                     RRRVLQGEQVPADEKLVSLFEPETAVIRKGKLSKPTEYGRMIWLDEVEGGIISRYEVL
                     VGNPSDSLQIQPSIAHHQQTFKRPPRLITADRGCWSPENEAAAQAAGIEHVCLPQHGS
                     KNAERKKHEAAGWFKRGCAWRAGIEGRIHGLKVRHKLNRCLYHGSAGMERWVGWGVIS
                     HDLRQIAQATAARA"
BASE COUNT          439 a          660 c          687 g          516 t
ORIGIN      
        1 ccgcagacgc ggcagaagtc gcggaaccgc gccaaccatt ccggctctgc ggagtagttc
       61 ttgtgcaact cggactcggc cctatagaac tcatacgtgt ccggtacatc ggtcagcgta
      121 ccatcgagca ggacccgctc attgggttcg agtctgcgca agaaggaatc cagaaacgtg
      181 gcgatctgct cggcctgctc gacagtcatc tcggcgtaga gcgaggactc agggatggga
      241 ccgatgcgct ctaaatcgag gatcgacgcc tggcgaagca acgtcacgac tgggcgccag
      301 gtccaccagg aggctttgag tgcttccacc gtctcctggt ttgggattaa ccacacccat
      361 gctgcctccg aatgggaatt aaaaccctag atagatgctg gtgcgaaact cacgtaaagc
      421 actctaaagc cttttggcgg ttcgcgtggt actttaacaa gctagactct tttcgctgat
      481 ttaggctcgg gcagctgttg cctgggctat ttgccgcaaa tcgtggctaa ttacgcccca
      541 gccgacccag cgttccatgc ctgccgaccc gtgatataag cagcggttca gcttgtggcg
      601 caccttcagt ccgtggatcc gcccttcgat ccccgctcgc cacgcacagc cccgtttgaa
      661 ccagcccgcc gcttcgtgct tcttgcgttc ggcattcttg ctgccgtgtt ggggtaaaca
      721 gacgtgctcg atgccggctg cttgggcggc cgcttcgttc tctggactcc agcagccccg
      781 gtcagcggtg attagcctgg gcggtcgttt gaaagtctgc tggtggtggg cgatgcttgg
      841 ctgtatctgt agactatcgg acgggttacc gaccagcact tcgtaacggc tgatgatgcc
      901 gccctcgact tcatctagcc agatcatccg cccgtattcg gtgggcttac tcagcttgcc
      961 tttgcgaatc acggccgttt ctggctcgaa caggctgact agcttttcgt cagccggcac
     1021 ctgctcgcct tgtaatactc gccgccgggt ctgtgtaagc accgcttgta agcgcggcat
     1081 aaattgcttg agcttgttcc tcagtgtgtc cgcttggcgt tggtgctgga ctgccaatag
     1141 ctcgccgact gcttgggctt gtgcgattag cgcatcgccg atctctacca ggcgctcata
     1201 actggctttg cgtacttgtt cggcctgctc accacggcga cgtacggtat cgatgatctg
     1261 cttattgagc cgtttggccg aacgggtacg gtcccgaaac agttgctggg ccgcgtcgcc
     1321 cgctgagatt agcacctcct gggccgactt caagatgcga ctgatgaccc ggatcccgtc
     1381 actgatgagg ctgctatcgg tgggctgatg gacgttggtt tcaaccaccg taccatcggt
     1441 gcggagcttg cggccacgcg taacgcgcaa ttggcaggcc acttcgacca catggtctag
     1501 caattgctgc aatgtgccgg gttggatgag attcgcccag cgcagcaggg tggtatcgtc
     1561 gggtaccggt tgccagtaga tgcggcagaa gtcccgcagg accaagctgt cgctgaccca
     1621 ttgctcggtt tgttcatagc tccagccata gaggtgcttg atgatgagca tccgcaggat
     1681 gacctcaacc ggcgtcgaag gccgcccagt agcgagggta tggggataac gctggctcag
     1741 atcggcacgc acctgctgaa ggatgacgtc atcgtcaagc agagtgtcga gttcggcgat
     1801 tacgggttcg atcgcatggc tcaattgggg taccatctgg aagacatcga cgggatcata
     1861 gcgatcacgt aacatggccg gcctcctttg cttggctcaa ggcgcagcag tgaacctgca
     1921 aatcggaggc cacccagagt tttgcaccag catctaggct aggttagaac tgccgctgtt
     1981 cccacggggc acgccgcagt gatgttaagg gcaccgcctg accaggcggt gcccagagaa
     2041 cacgcctcgc ccaaccagca gctgcgtcgc tgccgcttac aacggtcgcc ggtgccggct
     2101 ttggccgggc cgataaactg gtggcacttt ggagcaagga cccgccgaat tccacaatgg
     2161 tcggaggggt gtgattctgc gtgctgattg cacaaccggt cggataggag aaattacgaa
     2221 tgctgaatgc tgaattcctg acttgtgcaa ccggttgcac aagccttagg ttaaagggct
     2281 gggcgtgagt cagcgggaga tt
//
LOCUS       DASSFD010000148         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1835095, whole genome shotgun sequence.
ACCESSION   DASSFD010000148 DASSFD010000000
VERSION     DASSFD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835095"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..644
                     /locus_tag="VFH39_03500"
     CDS             189..644
                     /locus_tag="VFH39_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6924866.1"
                     /translation="MRKASRAIIIKDDKIIVTHRNKFGMQYYILPGGGVDMGETLEQA
                     LYRELAEEVCVEVANPRLVFTEEAGEPYGTQYVYLCDYVSGEPALHPDSAEAKIHALG
                     KNLYEPRWLSLAEFATIKLRSESLKQAILEGIKNGWPTEPTDVTGKANA"
     gene            683..1207
                     /locus_tag="VFH39_03505"
     CDS             683..1207
                     /locus_tag="VFH39_03505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6924867.1"
                     /translation="MALLFLASASALVIALAVQSYQNRHQGNSTNSTATDNSQQTNQS
                     QNKEGKLQGTKLSGFTPETSPYDKLQTIDLKEGTGDTVKPGDTVTVDYTGAVASTGVI
                     FQSSLDTGQPVSFPLSNVIRGWQEGIPGMKVDGTRRLLIPAALAYGANPPQGSGIPAN
                     ADLVFDVTLHKIGQ"
     gene            1433..1681
                     /locus_tag="VFH39_03510"
     CDS             1433..1681
                     /locus_tag="VFH39_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET6924868.1"
                     /translation="MVKNITIFTTNTCAYCAMVKKYLDAKGRGYDVVNLDEQPHRQAE
                     ALQLSGALTVPVTVVTKQDDSQEVIVGYNLAKLAPAVA"
     gene            1826..>2436
                     /locus_tag="VFH39_03515"
     CDS             1826..>2436
                     /locus_tag="VFH39_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET6924869.1"
                     /translation="MKENIIVYGADWCADCHRARYLFDKHGVKYEYKDVDSDPQIMNE
                     MLSLADGKNIIPTIQFGDGSVLQEPSNDELLKKLGLAEDSPMKNSHDVVMVGAGPAAL
                     TAAIYTTREDIDTVLFERGVVGGLAAVTDKIDNYPGFPDGVEGLTLADALQKQAERFG
                     AAIELGDVLKIHDEGMYKRLETTDGDYFARAVLIATGSDYKKIG"
BASE COUNT          634 a          664 c          675 g          463 t
ORIGIN      
        1 caaaagcggc ggcatttatg cccagctgct caaactgcag gagcagcaca ccgaaaccac
       61 caagcagaaa ctgcaagcat acgaaatctc cgcctagcct cttttccgca gataaacaat
      121 tattcacaat cataaacaaa atgtttactg tatggcgctg taagcatgat ttgctatgct
      181 taggtggtat gagaaaagcc tcgcgggcca taattattaa agacgataag atcattgtca
      241 cgcaccgtaa caagttcggg atgcagtact acatactgcc cggcggcggc gtggacatgg
      301 gcgagactct cgagcaggcg ctgtaccgcg agctggccga agaggtgtgc gtggaggttg
      361 ccaacccgcg gctggttttc acggaagaag ccggcgaacc gtacggcacg cagtacgtat
      421 acctgtgcga ctacgtgagc ggcgagccgg ccctgcaccc ggactcggcc gaggccaaga
      481 tccacgccct gggcaagaat ctgtatgaac ccaggtggct gagtctggcg gaatttgcta
      541 ccataaagtt acgttcggaa agtctcaagc aggcgatcct agagggaata aaaaacggtt
      601 ggccaaccga gccgacggat gtaacaggaa aggcgaacgc ataatgtcga cgagtaagac
      661 cacgcggttt ttcatgggtt tcatggcgct gttattcctg gcgtcggctt cggcgctggt
      721 catcgccttg gcggtgcaga gctatcagaa ccgccaccaa ggcaacagca ctaacagcac
      781 ggccacggat aattcgcagc aaaccaatca aagtcaaaac aaggagggta aattgcaagg
      841 caccaaactc agcgggttta cgcccgaaac ttcaccgtat gacaagctac agaccattga
      901 tctcaaagag ggcacgggcg acaccgtcaa gcccggcgac accgtcaccg tagactacac
      961 cggagccgtt gccagtacag gcgtcatctt ccagagctcg ctcgacaccg gccagccggt
     1021 cagctttccg ctcagtaacg tcatccgggg ctggcaagag ggcatacccg gcatgaaggt
     1081 cgacggcacc cgccggctgc tcattccggc cgctctggcc tacggggcga acccgccgca
     1141 gggatcaggc ataccggcta atgccgactt ggtcttcgac gtcactctcc acaagatcgg
     1201 ccaatagata gcttctgctg ggcacacctg gggcgaacag ctgcgtctct ttcccctcgt
     1261 gccttacagt atgctcgctg gtgggataat ttggggtaaa aagcacaata tgtagcgtat
     1321 tgcagttttc ataagcacta tatataatct ttttcacaga cgaacgccgt gcgcttagcc
     1381 taaaatacaa gcagctgaca aagcaaaaaa tataagggaa aagaggtcaa aaatggtcaa
     1441 aaacatcact atttttacta ccaatacctg cgcctactgt gccatggtca aaaaatacct
     1501 tgacgccaag ggcaggggct atgacgtagt caatctggat gagcagccgc accgccaggc
     1561 cgaggctttg cagctctcag gcgccctcac cgtgcccgtt accgtggtga ccaaacagga
     1621 cgacagccag gaagtgattg tcggctacaa cctcgccaaa ctggccccgg ccgtcgccta
     1681 agaaataaac ttaaagattt cgtcattcca aatttgattt ggaatccagc ccgtaagcat
     1741 ggccgcaggc catcctggag tagcctggat cccggccttc gccgtgatga caactgctgg
     1801 tagaatggaa tagcaaatgg ctggtgtgaa agaaaacata atcgtttacg gtgccgactg
     1861 gtgcgccgac tgccaccgtg ccaggtacct gtttgataaa cacggtgtca agtatgagta
     1921 caaagacgtc gacagcgatc cgcagattat gaacgagatg ctttcgctgg cagacggcaa
     1981 gaatataatc ccgaccatcc agttcggcga cggcagtgtt ctgcaagagc cgtccaatga
     2041 cgagctgctt aaaaagctcg gcctggcaga agacagtcct atgaagaaca gccacgacgt
     2101 ggtgatggtg ggtgccggcc cggcggcgct cacggcggcc atatacacca cccgcgaaga
     2161 catagacacc gtgctgttcg agcgcggcgt ggtgggcggt ttggcggctg taactgacaa
     2221 gatagataac tacccgggtt ttccggacgg cgtggagggg ctgacgctgg cggatgcgtt
     2281 gcaaaaacag gcagaacgct tcggcgccgc catagagctg ggcgacgtgc tcaagattca
     2341 cgatgagggc atgtacaagc ggctggaaac caccgacggc gattatttcg cccgcgcggt
     2401 gctcatagcc accggcagcg actacaaaaa gatcgg
//
LOCUS       DASSFD010000149        18983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1402203, whole genome shotgun sequence.
ACCESSION   DASSFD010000149 DASSFD010000000
VERSION     DASSFD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402203"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VFH39_03520"
     CDS             complement(<1..994)
                     /locus_tag="VFH39_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924870.1"
                     /translation="MPGQAEAVTTASSSCIKPYLAANKTGLQATPASWMGQIGALQPL
                     PGHPSWDGADNAQTGAYLASTDEMFVGGVAASYVHDGTSTAVHNLFAVKASTGALVTS
                     FLPNVNGAVTGMTLSCDGTGLYIVGTFTQVNGQARNHAAEVSVANGQLLPWNPHPDGT
                     PYDVAVVAGHVLVGGNFHHIGGAAHAYISAVNPTTGRAGNWVHVTISGPVNSGSVLVG
                     RIIGSPDQSKIALLGNFLNANGTYHSRIAFLRVTTRAARLLPWATNYTKPTGYQGCNP
                     NFGWKERDGMFSPDSQTFREAATGGYAKPGKYGTTYRSICDSVTAWDVSATALRN"
     gene            1304..2386
                     /gene="ychF"
                     /locus_tag="VFH39_03525"
     CDS             1304..2386
                     /gene="ychF"
                     /locus_tag="VFH39_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET6924871.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNALTNNDVLAANYPFATIEPNTGIV
                     PVPDGRLQCLSELYHDAPVKPATVTFVDIAGLVAGASKGEGLGNQFLANIRTTDAICQ
                     VVRAFRDPNVVHVDEQHDPKKDIDVINTELVLADLQTVERRLPKTEKEAKANPKLKPI
                     ADTLQRVHQLLDAGTPLSAVDDIDREHIADLQLLTAKPVIYVFNVDEDGLQDEARKKE
                     LAALVAPARSLFLCAKLEHELQSLDEDDARELLLSYGQDEPGLNQLIHAAYDTLGLQS
                     YLTAGDKEVRAWTIHKGATAPQAAGAIHGDFERGFIAADVVDYHDLLAAGSLATARAA
                     GKVRTEGKTYVMQPDDVVEFKFNVSK"
     gene            complement(2452..3219)
                     /locus_tag="VFH39_03530"
     CDS             complement(2452..3219)
                     /locus_tag="VFH39_03530"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6924872.1"
                     /translation="MGTNQNEGGDMADLKTLEERLKEIGCNFRFWGRAELRELANVLM
                     PNEIVEHCVNGQYQAGFAMLVATDQRVLLIDKKPMYLSLEDIRYDMMSEVDFNHRLLD
                     ASIFICTPTKSLRFVAYNQARLRKLFHFVQMRVMEIRQHYQQLAAQQAQQMQQLQQLS
                     QIPQPMAAVVQQTFSTDQPAPAIGSEPMPQIAAAPTQAQPVPQPTALPMMPPHAAPII
                     SAYTRLPTLARQRRFLGSRAIHHGPLSSIYSGQQPVG"
     gene            3380..4042
                     /locus_tag="VFH39_03535"
     CDS             3380..4042
                     /locus_tag="VFH39_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6924873.1"
                     /translation="MLATIKQTAELVEVFRTGGRQLTFKKGEFIIRPGEAPSGVFYIY
                     KGLVKAYDITKYGEENLLIIRKEHELFPLIWAITGQERHVIYEAIAPTIVWHINRDKF
                     VDYMQSHPEALAPLLDMTIEMYRLHSERILNLEYRSVRERIISFLLTMAQRFGKRTEA
                     GTTITVPLRHQDIASSINTSRETASRELAAMERKGFISSQQSLITICDLKGLRAQLDL
                     TE"
     gene            complement(4048..4488)
                     /locus_tag="VFH39_03540"
     CDS             complement(4048..4488)
                     /locus_tag="VFH39_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924874.1"
                     /translation="MFIEQSKPVQEADQPVNLVKPVPVDLTKHPEPEEPMTQAAVPRL
                     ATSGPRPRPMPERIKRKRDELDDDDDFSIWKMALWAGGPVLACAIGVMGVDSVFDTHI
                     AQDMAHIRVDTHAHGFLGDIKQEVERAVRGIGNCAVKLENGSDC"
     gene            complement(4539..5162)
                     /locus_tag="VFH39_03545"
     CDS             complement(4539..5162)
                     /locus_tag="VFH39_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924875.1"
                     /translation="MLAAKLVPLYRYEDCAVLALGDGGVVVGAQIASELHTVLTMLMI
                     EEINLPMEPEAIGGISADGSFSYNPAYKPGEIEELSGEFFQYIEAEKLTKLHDMNRLV
                     GDGGLISPELLRGRNIIVVSDGLKTSFALDIALQYLKPVSYKKLIVATPLASVRAVDR
                     MHVEADELYCLSVVEDYIDTDHYYDQHDIPPHEKVVEIIEKIILNWK"
     gene            complement(5269..5997)
                     /locus_tag="VFH39_03550"
     CDS             complement(5269..5997)
                     /locus_tag="VFH39_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6924876.1"
                     /translation="MTHSVKQRLDQALVSRGLVATRSQAESYIRMGDVHVNGSVVTKA
                     GYLVGDEHKIVLTAREQYVSRAAFKLASVAELLQLDFTNKTVLDVGSSTGGFTEYALR
                     HGARKVIAVEVGKDQLHPSLHGNLQIELHEQTDIRDFKTSEKIDLVVGDVSFISLREI
                     LPSVAKLATKQTFIAIMVKPQFEAAQSSLKHKGVIKNDRVRRQILKDFEDWARQRFVI
                     LDKADSHVAGSKGNVERFYLLKIS"
     gene            complement(5994..6308)
                     /locus_tag="VFH39_03555"
     CDS             complement(5994..6308)
                     /locus_tag="VFH39_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6924877.1"
                     /translation="MNSYFVYILTNRARGTLYIGVTNNLERRLAEHRSGEAEGFTKRY
                     ALHELVYFEESSSAEDAIAREKQLKRWHRQWKINLIEESMNPDWKDLSPRSRMDAETS
                     SA"
     gene            complement(6333..6812)
                     /locus_tag="VFH39_03560"
     CDS             complement(6333..6812)
                     /locus_tag="VFH39_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET6924878.1"
                     /translation="MLPGDRLVCAFMEPHAVGFRFERWLLHVTVVPWFRLDEASEVIA
                     SGLQKALHDIKPFEVALVERAMFGPRKNRPALLIGRPTPFDDIEPKVRGYLHKKRAWL
                     VDETTKRPRQFRPHVTLQKDSALAIGESFKVHTLYIVEQKDDYKEIREEIRLRGPSS"
     gene            complement(6856..7527)
                     /locus_tag="VFH39_03565"
     CDS             complement(6856..7527)
                     /locus_tag="VFH39_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924879.1"
                     /translation="MPGFEMRWGHGMDDAALRDAMRPVFRQGLNIAEERLEDVGLPLD
                     ETKRAVFHKVLCGYPYYAQTTTAKTAHFYFNIRELKKGIDVGLLGEVAGHEGAHMWRR
                     QVALVRTLGDKAVDEGLGYHAPLASRHPLRSLHSPPDAIVRKIHRLPKREVRAMSQRF
                     AEVIDEPLVADSAVVRRWERPYKDMVLSTFEVVGIAAVAARLDAGDAIANLMRLTTKQ
                     VLGIE"
     gene            complement(7603..7902)
                     /locus_tag="VFH39_03570"
     CDS             complement(7603..7902)
                     /locus_tag="VFH39_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924880.1"
                     /translation="MSLHDYAQLFAILGLFLVALSAVTFFLPIKLRTGYTSLWMFEDL
                     LNPTSRAKKWLLSDLKDPRVKVGGLLGLVIYGFVIGTGLLAIGGILWLIWYVVER"
     gene            complement(7915..8481)
                     /gene="efp"
                     /locus_tag="VFH39_03575"
     CDS             complement(7915..8481)
                     /gene="efp"
                     /locus_tag="VFH39_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6924881.1"
                     /translation="MALSITNLKKGVLFQWEGQPFRVVDYNQKVMGRGGSIVNVRIKS
                     LIDGKVLEKTFKGNEQLESADVTNQNVQYLYTDGSTYFFMNGDTFEQFEVPADLVGDG
                     AGYMKEGDVVQLQFFDGRPINVELPKNVPLLVTYTENAVKGDTTSSIQKDATLETGIT
                     IKVPAFIKQGDVISVDTSTGAYRERVKE"
     gene            complement(8482..8769)
                     /locus_tag="VFH39_03580"
     CDS             complement(8482..8769)
                     /locus_tag="VFH39_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924882.1"
                     /translation="MVVSLKPGNFMGLNGAAPGIIGSTDSGGDTILQLQRAAIEFSQL
                     YQTQGKDDELDSARRKLAGKIMVAVTLGALSERKADELMSAMSKLMKERKQ"
     gene            complement(8798..9127)
                     /locus_tag="VFH39_03585"
     CDS             complement(8798..9127)
                     /locus_tag="VFH39_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924883.1"
                     /translation="MDDSPFLAIKSLETQLSGVLSRLDLFELPKAEQKLVDTLRRNVA
                     DARLDIRDYELSETRDEQLRKAQAAKKRLERVRKGVLAASEYNVFGAVDVAQLTAQLE
                     QISDNLK"
     gene            complement(9166..9585)
                     /locus_tag="VFH39_03590"
     CDS             complement(9166..9585)
                     /locus_tag="VFH39_03590"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6924884.1"
                     /translation="MTEEDAIMSTSTKQTTMAVRTFRDLRVWQAAYDMTVALYRQCEQ
                     FPKHELYALTNQMTRAAVSVCSNIAEGFGRRSLREKDQFYAMANGSLTELENQLLICR
                     GVGYITSAQFLDLQRRCYATHKMLVALQNVNRSKSDH"
     gene            complement(9673..9876)
                     /gene="rpsR"
                     /locus_tag="VFH39_03595"
     CDS             complement(9673..9876)
                     /gene="rpsR"
                     /locus_tag="VFH39_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6924885.1"
                     /translation="MAKIFKHKTDVAFFDYKDFKTLQRYVNQYGQIETRKKTGLTESQ
                     QRRLSVAIKRARHIALLPFVASN"
     gene            complement(9897..10199)
                     /locus_tag="VFH39_03600"
     CDS             complement(9897..10199)
                     /locus_tag="VFH39_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924886.1"
                     /translation="MNTLTECHPDNRCVTCPVALLKLQDQLRASGLIEAVMASGSLSS
                     ASSPEDVVTNVKAEADIQEVDQLAGIAVGAVAYRLSGRCPQVVRDGERRTVLIKEL"
     gene            complement(10301..10804)
                     /gene="ssb"
                     /locus_tag="VFH39_03605"
     CDS             complement(10301..10804)
                     /gene="ssb"
                     /locus_tag="VFH39_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6924887.1"
                     /translation="MARSLNQVTLMGNLTRDPELRQTPTGQNVCSFSLALNRAYKDSS
                     GEWQEATDYIDIVAWGPLAERVAQYLSKGRRCLVQGRLQSRSWEQDGNKRSKVEVLAN
                     DVTFLDSRGEGGGEGGGFSGGSNYSGGNGGGSSSSSSKPAASKKKDDVVIEDVGDEPI
                     NLDDIPF"
     gene            complement(10897..11277)
                     /gene="rpsF"
                     /locus_tag="VFH39_03610"
     CDS             complement(10897..11277)
                     /gene="rpsF"
                     /locus_tag="VFH39_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET6924888.1"
                     /translation="MNQYEIAVLYHPDLEIDLDKAASRVTKIFTDNGGKVVSEDNWGK
                     RKLAYDIKGNEHAVYVFYTVELPGEAVRRVESTLNITDEVIRFLITKPDLKAIAKAEA
                     EKEAKAAKAAARGETGDDEAKDEE"
     gene            complement(11381..12124)
                     /locus_tag="VFH39_03615"
     CDS             complement(11381..12124)
                     /locus_tag="VFH39_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924889.1"
                     /translation="MAPEFITPLDHATGIPFPLMPRVDLPPVPVGRIKNEARIADWHH
                     PFHPRQELVSEGITSAVAVRNCRIQWAEYDDHHHKYHGAYFGPVLPETDIDRFRTVVF
                     AAAGYVPDAAIAFGRFHRPLIKKLDEDQRNYLWHSGQIRIANFATVRDYLLEYTLASD
                     LSGVRESTIDEFLHTSDVKRRKELGGTLLGIAAYRAAAPVNEVYKLSRKMNLIPPDRA
                     RTAGRFILKTMNTQRQKKAMSALMTKLAA"
     gene            complement(12371..14596)
                     /locus_tag="VFH39_03620"
     CDS             complement(12371..14596)
                     /locus_tag="VFH39_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET6924890.1"
                     /translation="MAKKKKSSKKKVAAPPPKEPSVFWPLAGAFILILLALFLLLGGF
                     GTGGTLPKGMYHGAYATFGWAAALTPFALAYWGIYKLRSDERRLPVAKLSSVVCTLVF
                     AAAFFGTMFAHVAADDAGGKAVTGVHGGAIGNAFAQGVLGMLDKLPASLLFLLLTLLA
                     ICFALGASPKVLLRPFEHSFRRRERPEGEEITDLAQLKAKATGGFKLNEGVPVEHHSE
                     EPAKEPVKLASFRNTAQKLTPSENHAALTAASDPDWQFPSLTRLNQKQDKADPGNVEH
                     NARLIHDTYANFNIDVEMEGANVGPRITQYTMKPPTGVKLQKITALENNLALDLAATS
                     IRMEAPIPGKRAVGIEVPNVKPATVRLSALLTSKQWSDISGPLGFAIGRDISGTPVVA
                     DLAKMPHLLVAGQTGSGKSVMINTILTSLLYRNSPADLKLIMVDPKFVELGTYNDIPH
                     LLTPVITEPEKVISALKWAVAEMERRLRTMAEINTSNIAEYNAAKKEEGMPYIVIVID
                     ELADLMMMAARDVEALIVRIAQKARAAGIHLILATQRPSVNVITGLIKANVPARIAFT
                     VASQVDSRTIIDGVGAEKLLGMGDMLFYTTDMAKPKRVQGALIEKGEVVKVTDFVKQQ
                     RAAEYNDEVVSQPVQLDGKGGVVADMSAGGDADDDMFRDAVRVVIEGHKASTSLLQRR
                     LRIGYGRAARLIEEMEEQGIIGAADGSRPREVLVSSLDQVFGGGEAAPSDEPAEQLIQ
                     G"
     gene            complement(14633..15049)
                     /locus_tag="VFH39_03625"
     CDS             complement(14633..15049)
                     /locus_tag="VFH39_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924891.1"
                     /translation="MSHNEGMPPEESPSTEPSQEPGTQQGEAVNEEFIHLESEQAADA
                     LRQFVETAEEEELQPFRELVRDALLRLYEVSVQPSAETTERAQAEWVEALLAHRRLID
                     PNLRGDEPVDPEELNQASERLRRARQNLDALERPEG"
     gene            complement(15096..15314)
                     /locus_tag="VFH39_03630"
     CDS             complement(15096..15314)
                     /locus_tag="VFH39_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924892.1"
                     /translation="MTVEAPKDINQLSEPVIEVADNVEEESLPPLPPELARRHDDFVR
                     QYTDPARRLGAHQLARQALDRKMRRGGW"
     gene            complement(15311..15700)
                     /locus_tag="VFH39_03635"
     CDS             complement(15311..15700)
                     /locus_tag="VFH39_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924893.1"
                     /translation="MKTISEAAHPMSHDWNGHPLDATVTYPRTESDDIAEMAERFHDD
                     LERETGPQGNIFGLARHESISQTTNTEVTPRVTRPLLPRNHVEDGVTERNSDKISDEQ
                     WMIFKVSADEGRPNFLQGLRRARKKPR"
     gene            complement(15758..17917)
                     /locus_tag="VFH39_03640"
     CDS             complement(15758..17917)
                     /locus_tag="VFH39_03640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET6924894.1"
                     /translation="MEPSNNDNNQEQQGRRPQGQRPAQNNRRGNGGQNGRPAANGRGR
                     GGNNHGGQNRQNQPKDQNNGGERRSGVSRGAAVRAQKRSQMDAQRIANQYTAAQVSPE
                     QKRANFIDDSPRLKIIGLGGMDGGGSKNMMLVEYVNDAIVIDAGNDLSVDLPGINYGV
                     ADTAYLETIRHKLRAYVITHGHLDHIGGLPHIVPKFPAPIYGSRFTIGRVEEIFENFG
                     LPMPDGFELKTVTMNEDTHERLKVGEFFVELVRITHSIPGSTCVVLDTPVGRIINTGD
                     FRFDPNPLDHERSDIERLKELGNEGVLALLSESTTVERLGRTPSESTIEQSFIDIMDQ
                     APGRIFVGLFSTNMNRVQMIINAAIHHNRKIAMDGRSMVSTLEMAVRHGFMKVPKGTF
                     VPIASAATMPDDQLVVVCTGSQGEPSSALQRMANGEHRHIKLKAQDTVILSSTPIPES
                     GNDALVGTMVDNMVMKNVHVFEARNHDLDHVGPLHVSGHASQDEYAEMIQYTKPKFFI
                     PIYGAYRVKQRHIDLAVEQGVPRANCVNALNGEVIALTPDKMEVIGEVPHGTILVDQT
                     GAIVSNVVVKDRVLLAEEGLVAVILTIDKKSGNLMTSPDIVSRGFIYMRDNEEIMNGL
                     RNELRRAVTQRFKRVDLDRFKAELKDHITHYLFEQTQRSPIIIPVVNVIGGGKGGNEA
                     KTVSGGRTKTAEEIALEQQKRFQQMRERLLTQDARVD"
     gene            complement(17955..18341)
                     /locus_tag="VFH39_03645"
     CDS             complement(17955..18341)
                     /locus_tag="VFH39_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924895.1"
                     /translation="MQKETFIQGLQRKFLRADFRADETVAWVQSNRMTRKFLKGDEYL
                     QSSNPMKLLYIVVNVIELVAEVVVIYMFIFRLAPEAFSHRVTYGFGGSGLLGQLLTVA
                     AFVFMLCLLPFTAASLRWMLKHKNTN"
     gene            complement(18341..>18983)
                     /locus_tag="VFH39_03650"
     CDS             complement(18341..>18983)
                     /locus_tag="VFH39_03650"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6924896.1"
                     /translation="TAYLESSAFMSTTTRQQALDELAQKIVDDEVTPQLQAQATQLVF
                     GDGNPSADIVFIGEAPGKNEDLQGKPFVGAAGKFLNEMLEGIGLERKDIYITNIVKYR
                     PPNNRDPLPDEKKAFLPYLQSQLEIIQPKVVVTLGRHSMNCFLPDLQISQVHGQPKRV
                     KLQLKEHAGDVLEVVILPLFHPAAALYNGGMRQTLIDDFAAIPVIIQKIKENK"
BASE COUNT         3762 a         5610 c         5185 g         4426 t
ORIGIN      
        1 tattgcgcag tgccgttgca gacacgtccc aggcagttac cgaatcgcag attgagcggt
       61 aggtcgttcc gtacttgccg ggcttggcgt aaccgccggt cgccgcctcg cggaacgtct
      121 ggctgtccgg cgagaacata ccgtcacgct ccttccaacc gaagttcggg ttgcaaccct
      181 ggtaaccggt cggcttggtg tagttggtcg cccacggcag taatctcgct gccctggtag
      241 tcacccgtag gaaggcgatg cggctatggt acgtgccgtt ggcgttcagg aagttcccga
      301 gcagcgcgat tttgctctgg tcggggctgc cgatgatacg gccgacgagc acggagccgg
      361 agttaaccgg accgctgatt gtcacgtgca cccagttgcc tgctctgccg gtggtcgggt
      421 tgaccgccga gatgtaggcg tgggccgccc cgccgatgtg gtggaagttg ccgccgacca
      481 acacgtggcc tgcgaccacc gcgacgtcgt acggtgtgcc atccgggtgc gggttccaag
      541 gcagcagttg tccgttggct acggaaactt cggccgcatg gttgcgcgct tgtccgttca
      601 cctgcgtgaa cgtccccacg atgtacagtc cggttccgtc gcacgacagt gtcatgccgg
      661 taaccgcgcc gttcacgttc ggcaggaacg aggtcaccag cgcacccgtc gaagccttca
      721 cggcgaacag gttgtgcacg gctgtcgagg tgccgtcgtg gacatagctg gcagccaccc
      781 cgcccacgaa catctcgtcc gtagaggcaa ggtatgcccc ggtctgggca ttgtctgcac
      841 cgtcccacga cgggtgtccg ggcaaaggct gcaaggcacc gatctgcccc atccatgaag
      901 caggcgtggc ttgcagtccg gtcttgttgg cggccaggta cggttttatg caactgctgc
      961 ttgcagtcgt caccgcttcg gcctggccgg gcatcaggta aaacgaaagg cctacgaacg
     1021 ctagcgccgt taatggcgct atagcgtttc gtatggctcg acgtaggcgc ataatgcacc
     1081 acccttctgt gtaagtgcgc tcccttttgg aacacagctt ccgaccgacc cactcgaatc
     1141 agccaaaagc cgtgttccat ttcaaacgat ctgcatgtaa atgtgcgcgt cagcggcgaa
     1201 caattctcac aataagaaaa gacgctccac cgagcgctgt gtatactcta taaaaatatt
     1261 ggcaatttgt caatgagtat tacctctgtt ataatggcaa ttaatgagtc tttctatcgg
     1321 catcgtcggc ctgcccaacg tgggcaaatc gacgcttttt aacgccctga ccaacaacga
     1381 tgtactggcc gccaattacc cgttcgcgac catcgaaccg aacaccggca tcgtcccggt
     1441 tccggatggc cggctgcaat gcttgagcga gctgtaccac gacgcccccg tcaaaccggc
     1501 caccgtgacg ttcgtcgaca tcgccgggct ggtagccggc gcgtccaagg gcgaagggct
     1561 gggtaaccag ttcctggcca acatccgcac caccgatgcc atctgccagg tggtccgcgc
     1621 cttccgcgat ccgaacgtgg tccatgtgga cgagcagcac gacccgaaaa aagatatcga
     1681 cgtcatcaac accgagttag tactggccga cctccagacg gttgaacgcc gcctgccgaa
     1741 gaccgaaaag gaagccaagg ccaacccgaa gctgaagccc atagccgaca cgctgcagcg
     1801 ggtacaccag ttactcgatg ccggcactcc tctgtcggcc gtcgacgaca tcgaccgcga
     1861 gcacatcgcc gacctgcagc tgctcaccgc caagccggtc atttatgtgt ttaacgtgga
     1921 tgaagacggc ctgcaggatg aggcgcgcaa aaaagagctg gccgccttgg tcgcacccgc
     1981 tcgcagcttg ttcctgtgcg ccaaactcga acacgagctg cagagcctcg acgaagacga
     2041 tgcccgggag ttgctgctaa gctacggtca ggacgaaccg ggcctgaacc agctgatcca
     2101 cgccgcctac gacacgctcg gcctgcaaag ctacctgacg gccggcgata aagaagtccg
     2161 cgcctggacg atacacaaag gagccaccgc accccaggct gccggtgcca tccacggcga
     2221 cttcgaacgc ggctttatcg ccgccgacgt cgtcgactat cacgacctgc tggctgccgg
     2281 ttcgctcgcg accgcccggg ccgccggcaa agtacgcacc gaaggcaaaa cgtacgtcat
     2341 gcaaccggac gatgtcgttg aatttaagtt caacgtatca aaataggacg agtcatgccc
     2401 cataaagaaa cccgcctaac aaagtgggct tcttttattg aagcgctcgc gctacccgac
     2461 gggctgttgc ccggagtaga tgctcgatag cggtccgtgg tggatcgccc gggagcccag
     2521 gaagcggcgt tgacgcgcca atgtcggcaa gcgggtgtag gcgctgatga tcggcgccgc
     2581 gtgcggtggc atcatcggca aggcggtggg ctgcggtacc ggctgggcct gtgtgggggc
     2641 cgctgctatc tgcggcatcg gctcagaccc gattgcagga gccggctggt ccgttgagaa
     2701 cgtctgctgc accactgctg ccatcggctg cggtatctgg ctcagctgct gcagctgttg
     2761 catctgctgc gcttgctgcg ctgccagctg ttggtagtgc tggcggattt ccatcacccg
     2821 catctgaacg aaatggaaca gcttgcgcag ccgtgcctgg ttgtaggcga cgaaccgcag
     2881 gctcttggtc ggcgtgcata tgaatatgct cgcgtccagc aagcggtggt taaagtccac
     2941 ctcgctcatc atgtcgtagc ggatgtcctc cagcgacaga tacattggtt ttttgtctat
     3001 gagcagcacc cgttggtcag tggcgacaag catcgcgaag ccggcctgat actgaccgtt
     3061 cacgcaatgt tcaacaattt cgttcggcat gagcacgttg gctagctctc gtaattcggc
     3121 tctgccccag aagcggaaat tacacccaat ctccttcagg cgctcttcga gcgtttttag
     3181 atcggccata tcccctcctt cgttttggtt ggtacccata actatacggt tgagccgggc
     3241 aaaccgtcgg tgagaatgct aactaacttt gtgagatatt catcacacgt catatctaaa
     3301 gccccagccg agcgccgcat aagcggttta cagatacgaa aagctagcca tacaggcggg
     3361 gatgtggcat aatccatata tgttagccac tatcaagcag accgccgagc ttgtagaggt
     3421 gttccgaacg ggcggtcgac aactgacatt caaaaagggt gagttcatca tccgacctgg
     3481 tgaggcgccg tccggcgtct tttatatata caaggggctg gtcaaagcct acgacatcac
     3541 caagtacggg gaagaaaacc tgcttatcat ccgtaaggag catgagttgt tcccgctcat
     3601 ctgggccatc accggccagg agcgacacgt catatacgaa gccatcgcac cgaccatcgt
     3661 ctggcacatt aaccgcgaca agtttgtcga ctacatgcag tcgcacccgg aagcgctggc
     3721 tcccctgctg gacatgacca tcgagatgta ccgcctgcac agcgagcgca ttctgaacct
     3781 ggagtaccgc agcgtacgcg agcgcatcat ctcgttccta ctgaccatgg cgcagcgttt
     3841 cggcaaacgc accgaggccg gcacgaccat aactgtaccg ctccgccacc aggacatcgc
     3901 cagctctatc aacacctcgc gcgaaacggc cagccgtgaa ctggccgcca tggaacgcaa
     3961 aggatttatc agcagccagc agtcgctcat caccatctgc gacctcaaag gtttacgcgc
     4021 ccaactcgac ctcaccgaat agcgtattta gcagtcgctg ccgttttcta atttgaccgc
     4081 acaattccca ataccgcgca cggcgcgctc aacttcttgc tttatatcac ccaggaaacc
     4141 gtgggcatgc gtatcaaccc gtatatgggc catatcttgt gcgatatgag tatcgaatac
     4201 cgagtccacc cccatgacac caatcgcaca cgccaacacc gggccgcctg cccatagcgc
     4261 cattttccat atggagaaat catcgtcgtc gtcaagttca tcgcgttttc gtttgatgcg
     4321 ctccggcatc ggacggggtc gcggacccga agtggccagg cgcggcacgg cggcctgagt
     4381 catgggttct tcaggctcag ggtgcttggt aaggtcgacc ggcacaggct taaccaggtt
     4441 caccggctga tcagcttctt ggacaggttt gctctgctct ataaacatgc ctgcaaccat
     4501 aacacaaaaa tgctgatatg actagcgcta cggctcgact atttccagtt aagaataatc
     4561 ttctcgataa tctccaccac cttctcatgc ggcggtatat cgtgctggtc atagtaatgg
     4621 tcggtgtcta tgtaatcctc gacgacgctc aagcaataca gctcgtcggc ctcgacatgc
     4681 atgcggtcca ccgctcgcac gctggccagc ggggtggcta cgatcagctt cttgtatgag
     4741 accggcttga ggtactggag cgcgatatcc agggcaaacg acgtcttgag gccgtccgac
     4801 accacgatga tgttccgccc ccgcagcaac tccgggctta tcaagccgcc atcacccacc
     4861 aagcggttca tgtcatgcag cttggtcagc ttctcggcct cgatgtactg gaagaattcg
     4921 cctgacagtt cttctatctc gcccggttta taggcagggt tatacgaaaa cgacccgtcc
     4981 gcactgatgc cgccgatagc ttccggctcc atgggcaggt ttatctcttc gatcatcaac
     5041 atggtaagca ccgtatgcag ctcggaggcg atctgtgccc cgaccaccac ccctccgtca
     5101 ccgagtgcca gcaccgcgca atcttcgtac cgatacaacg gcaccagctt ggcggccaac
     5161 atccggccag cttgcatacg gctcgcgaaa tacacttaca cccggccttc tcgggcgtaa
     5221 ctgccagcat gcccaactac gcttatggtt gtcatttgct ttattttatc agctaatctt
     5281 cagtaggtag aagcgttcga cgttgccctt actacccgcg acgtgcgaat cggctttgtc
     5341 caggatgacg aagcgctgcc gagcccagtc ttcgaaatct ttaaggatct gccgccgcac
     5401 gcgatcattc tttatgacgc ccttgtgctt caggctgctc tgcgccgctt cgaactgcgg
     5461 tttgaccatg atggctatga acgtctgctt ggtcgccagt ttagccacac tcggcagtat
     5521 ttcccgcaaa cttataaagc tcacgtcacc caccaccagg tcgattttct cgctggtttt
     5581 gaagtcccgg atgtccgtct gttcgtgcag ctcaatctgc agattgccgt gcaggctggg
     5641 gtgcagctgg tctttgccga cctccacggc gatgaccttc cgggcaccgt gccgtagcgc
     5701 atattccgtg aatccgcctg ttgaggagcc cacgtccagc actgttttgt tagtgaaatc
     5761 cagttgtagc agttcggcca cgctggccag tttgaacgcc gcccggctca cgtactgctc
     5821 acgcgcagtc agcacgattt tgtgttcatc ccccaccaga taaccggctt tggtcacgac
     5881 cgaaccattc acgtgcacat cgcccatgcg tatgtagctc tccgcctggc tgcgtgtcgc
     5941 caccaacccc ctgcttacca acgcctgatc aagccgctgc ttcaccgagt gtgtcatgct
     6001 gaactcgttt cagcatccat gcgtgaccgc ggacttaaat ctttccaatc cggattcata
     6061 gactcctcta ttaggttgat tttccactgt cgatgccacc tctttaactg tttttctctt
     6121 gcgatagcgt cttctgcgct ggatgactct tcaaagtaaa caagctcatg cagggcgtac
     6181 cttttggtaa agccttcggc ctcgccgctg cgatgctctg ctaagcgacg ctcgagattg
     6241 ttcgtgacgc caatgtacaa ggtgccgcgg gcccgattcg ttaatatgta tacaaaataa
     6301 ctattcatgt tcgtatggat cctgaaacaa gttcaggatg acggccctct caaccttatc
     6361 tcttccctaa tctctttata atcgtctttt tgctcgacga tgtacagcgt atgcactttg
     6421 aatgattcac cgatggcaag ggcactgtcc ttttgaagcg tcacatgcgg acggaactgg
     6481 cgggggcgtt tggtggtttc gtcgaccagc caggcgcgct ttttgtggag atagccgcgc
     6541 accttcggct cgatgtcgtc gaacggagtc gggcggccga tgagcaacgc cggccggttc
     6601 ttgcgcggcc cgaacatcgc ccgttcgacc agcgccactt cgaagggctt aatatcgtgc
     6661 agtgcttttt gcaaacctga ggcaatcact tccgacgctt catccagccg gaaccacggc
     6721 accaccgtca cgtgcagcaa ccaccgctca aagcggaacc cgacagcgtg cggctccata
     6781 aacgcacaaa ccaacctatc gcctggcagc atactatgta ttgtacggga agcaccgggc
     6841 gttatcggga cccgactact ctatgccgag tacctgcttg gtcgtcaggc gcatcagatt
     6901 ggcgatggca tctcctgcat cgagccgagc tgcaacggcc gctataccca ctacttcaaa
     6961 agtactcagt accatatctt tatatgggcg ttcccatctt ctgactacgg cactgtcagc
     7021 caccaacggc tcatcaatca cctcggcaaa gcgctggctc atagcacgga cttcacgctt
     7081 tggcagccga tgtatttttc ggacgatggc atcgggaggt gagtgaagcg aacgcagggg
     7141 gtggcgcgaa gcaagcggag catggtaacc cagcccttcg tccacagcct tatcacctag
     7201 tgttcgcaca agcgccacct gtcgacgcca catatgtgcc ccctcgtgac cagccacctc
     7261 tcccagcagc ccgacatcta tacccttctt caactcacgg atattgaagt agaagtgcgc
     7321 tgtcttggcg gtcgtcgtct gtgcgtaata aggatagccg cataacacct tgtggaatac
     7381 agcacgcttc gtctcgtcga gaggcaatcc gacgtcttct aggcgctcct cggcaatatt
     7441 caggccctgc cggaacacgg gacgcatggc atcacgaagg gcggcatcgt ccatcccgtg
     7501 accccagcgc atctcaaaac ccggcataca gctatgatac gcctactata ttgccaagag
     7561 ggatgtatat aaaccaggcg cagcccacga ggtccaagca atctatcttt cgacaacgta
     7621 ccaaatcagc cagaggatgc cgccgatggc aagaagtcct gtaccaatta caaaaccgta
     7681 tataaccaag ccgagcaaac ccccgacctt cacgcgtgga tctttcagat cactaagtag
     7741 ccacttttta gcacgacttg taggatttag tagatcctca aacatccata acgatgtata
     7801 gccagtgcgg agctttatcg gcaggaaaaa ggttaccgcg ctcaatgcca ccagaaaaag
     7861 gcctagtatt gcaaacagct gagcgtaatc atgcaatgac atgaggtggt tatcttattc
     7921 ttttacgcgt tcgcggtagg caccggtgct ggtgtcgacg gatatgacgt cgccctgctt
     7981 gatgaacgcc gggaccttga tggtaatgcc ggtctcgagc gtggcgtcct tttgtatgct
     8041 gctggtagtg tcgcccttga cggcgttctc ggtgtaggtg accagcagcg gtacgttctt
     8101 gggcagctcg acgttgatcg gccggccgtc gaagaactgc agctgcacca cgtcgccttc
     8161 tttcatgtag ccggcgccgt cgccaaccag gtcggccggg acctcgaact gctcgaacgt
     8221 gtcgccgttc ataaagaagt acgtactgcc gtcggtgtac agatactgta cgttctggtt
     8281 ggtcacgtca gcactttcca gctgctcgtt gcccttgaag gtcttttcga gcaccttgcc
     8341 gtctatgaga ctctttatac gcacgttcac gatcgaaccg ccgcggccca tcactttctg
     8401 gttgtagtcc accacccgga acggctgccc ttcccattgg aacagcacgc cttttttgag
     8461 gttggtaatg ctcagtgcca tttactgctt cctttctttc ataagtttgc tcatggccga
     8521 catgagttcg tcggccttgc gctcgctcaa agcgccgagc gttacagcta ccattatttt
     8581 acctgccagc ttgcgcctgg ctgaatccaa ttcgtcatct ttgccctggg tttgatacag
     8641 ctggctgaac tcaatcgccg cccgctgcag ctgcaagatg gtatcaccgc cactgtcggt
     8701 actaccgatt atacccggcg ccgcgccgtt caggcccatg aagttgccgg gcttaaggct
     8761 gacaaccatt tccgtaaacg cgtgtatgtc catagtacta cttcaggtta tcactgattt
     8821 gttcgagctg ggcggtgagc tgggcgacat ccaccgcacc gaacacattg tattcgctgg
     8881 cagccaatac gcccttacgg acccgctcca gccgcttctt ggccgcctgg gctttgcgca
     8941 gttgctcgtc acgggtctcg ctcagctcat agtcgcggat atccagccgg gcgtcagcca
     9001 cgttgcgccg caaggtgtcc accagcttct gctccgcctt gggtagctcg aacaggtcga
     9061 gccgcgacag cacgccgctc agctgcgtct ccagcgactt tatagccaga aagggtgaat
     9121 catccataga atagattata gaagctggat tctagattat ggaatctaat gatcgctttt
     9181 actccggttc acgttttgca atgctaccag catcttatgg gtcgcgtagc atcgacgctg
     9241 caagtccagg aattgggcgg aagtgatata gccaacgccc cggcaaatca gtagctgatt
     9301 ttcgagctcg gttagcgagc cgttcgccat agcgtagaac tgatcttttt ctcttaatga
     9361 tcgcctgcca aagccttcag caatattcga gcagaccgat acggctgccc tcgtcatttg
     9421 gttcgtcaga gcgtagagtt catgtttcgg aaattgctca cattgtcgat agagcgccac
     9481 agtcatatcg taggcggcct gccatacccg taaatcccga aaggttctta ctgccatggt
     9541 agtttgcttc gtgcttgtac tcataattgc atcctcttca gtcaaaagct tagttatatg
     9601 tcggatattc cagcatccac cttctagaat ccagaatctc gtagcctacg gctacgaagc
     9661 aattagctaa tgctaattgc tggcgacgaa cggcagcaac gcgatgtgcc gggcgcgctt
     9721 gatagcgacg ctcaggcggc gctgctggct ttcggtcaga ccggtcttct tgcgggtctc
     9781 gatctgcccg tactggttca cgtagcgctg caaggtcttg aagtccttgt agtcgaaaaa
     9841 cgcgacatct gtcttgtgct taaaaatctt tgccattatt tactcctctg tactattcaa
     9901 agttccttaa ttaaaactgt tcgtctttca ccatcacgta ccacttgagg gcagcgacca
     9961 gacaaacggt aagctactgc gccgactgcg atacctgcta gctggtccac ctcttgaata
    10021 tctgcttcgg cttttacgtt agttaccaca tcctctggtg atgacgccga actaaggcta
    10081 ccgctagcca tgactgcttc tatcagtcct gaggcgcgca gctgatcctg taacttcaaa
    10141 agtgcaacag gacatgtgac acaacgatta tctggatggc attcggttag tgtattcata
    10201 tgtagctttc tcccactcca ctattcagga aagagcagct ggagaggcga attcttttcg
    10261 actcaccgcg tgatgagaga accgaggctc tctcaggctg ttagaacggg atgtcgtcca
    10321 ggttgatcgg ctcgtcgccg acgtcttcga tgacgacgtc atccttcttc ttgctcgcgg
    10381 ccggcttgct ggagctgcta gagctaccac cgccgttacc gccactgtag ttcgagccgc
    10441 cgctgaagcc gccgccctcg ccaccgcctt caccgcggct gtccaggaac gtcacatcgt
    10501 tggccagcac ttccaccttg ctgcgcttat tgccatcctg ctcccaactc cgggactgca
    10561 aacggccctg caccaggcag cgacggcctt tgctcaggta ctgtgccaca cgctcggcca
    10621 ggggacccca ggcgacgatg tcgatgtaat cggttgcttc ctgccactcg cctgacgaat
    10681 ctttgtatgc gcggttcagc gccaggctga agctgcacac gttctggccg gtcggtgtct
    10741 gccgcagctc ggggtcccga gtcaggttcc ccattagtgt tacttggtta agacttcgtg
    10801 ccatttcggc atcctccttc tgtgcgaaac tatgtcgcgg attagtttgt actactcacg
    10861 ttacgcttag gcctgagggg cgtcaagccg taaaatttac tcttcgtctt tagcttcgtc
    10921 gtcaccggtt tcgccgcggg cggccgcctt ggctgccttg gcttccttct cggcctcggc
    10981 cttggcaatc gccttcaggt cgggcttggt gatgaggaaa cggatgactt cgtcggtaat
    11041 gttgagcgtg ctctcgacgc ggcggacagc ttcgcccggc agttcaacgg tgtagaacac
    11101 gtacacggcg tgctcgttac ccttgatgtc gtaggccagc ttgcgcttgc cccagttgtc
    11161 ttcgctcacc acctttccac cgttgtcggt gaagatcttg gtgacgcgcg acgcggcctt
    11221 gtccagatct atttccagat ccgggtgata cagcacagcg atctcatact gattcatcag
    11281 cagttctcct ttggttaaag cctacgcaca catattcggt cgtaagctga gctaataatc
    11341 ccgttattct aacgtaatta cctctgttag tcaaccgggg ctaagcggca agcttggtca
    11401 tgagtgctga catagctttc ttttgccgct gagtgttcat cgtctttaat atgaagcgtc
    11461 ctgcggtccg cgcccgatca ggaggaataa gattcatctt gcgggacagc ttgtacactt
    11521 cgttcacagg ggccgcggcg cggtacgcag caatacccaa tagcgttcct ccgagctctt
    11581 tcctgcgctt cacatcggac gtatgtagga attcatcgat ggtagactcc ctaacgccgc
    11641 tcaaatcact agcaagggtg tactccagca agtagtctct aacggtagca aaattcgcga
    11701 ttctgatctg gccgctatgc cacagataat tacgctgatc ttcgtccagc tttttgatta
    11761 aaggtctgtg aaagcgtcca aacgcaattg ccgcatcagg gacatagccg gcggcggcaa
    11821 ataccaccgt cctgaaccga tcgatgtctg tttcgggcaa tacagggcca aagtaagcac
    11881 catgatattt gtggtggtgg tcgtcatatt ccgcccactg gatgcggcag tttcggacag
    11941 ctacagccga ggttatgccc tccgatacga gctcctgacg cgggtggaat ggatgatgcc
    12001 agtcggcaat ccgggcctcg ttctttatcc tgcccaccgg taccggcggc aaatcgaccc
    12061 gaggcattag cggaaacggg atacccgtag cgtgatccag cggcgtaata aactcaggcg
    12121 ccatgcgaga ccccctgtag cgttggtacg aaaaagctcc cggcagcggg agcttcagta
    12181 cgtaaggagt tttgctggtc gagggacggt gggagttgac cagtcgtttg gctgttgcca
    12241 gtgaactgct cagtcacctg ccgtccctcc gtttgcaagt tttggtgtta cgtttatggt
    12301 aagaaatctt acacacaaat gcaacccact tttttgttca actaccggtc gtatctgtaa
    12361 aaattacaaa ctagccctgg atgagttgtt cggccggttc gtcgctgggg gcggcctcgc
    12421 cgccgccgaa gacctggtcc aagctgctca ccagcacctc gcgcgggcgg gagccatcgg
    12481 cggcaccgat gataccctgc tcttccatct cctcgatgag ccgggccgcc cgaccatagc
    12541 cgatgcgcaa ccggcgctgc agcagcgagg tcgaagcctt gtggccttct atcacgacgc
    12601 ggacggcgtc gcggaacatg tcgtcgtcgg catcaccgcc ggcgctcatg tcggcgacca
    12661 cgccgccctt gccgtccaac tggaccggct ggctgacaac ctcgtcgttg tactcggcgg
    12721 cccgttgctg cttcacgaag tcggtcactt tcaccacttc gcccttctct atcagcgcac
    12781 cctggacgcg cttcggttta gccatgtcgg tggtatagaa cagcatgtca cccatgccca
    12841 gcagcttctc ggccccgacg ccgtcgatga tggtacgact gtcgacctgg ctggccaccg
    12901 tgaaggcgat acgggccggc acgttggcct tgatgagacc ggtaatgacg ttgacgctcg
    12961 ggcgctgcgt ggccaagatg aggtggatac cggccgcccg ggccttctgg gcgatacgca
    13021 cgatgagcgc ctcgacgtcg cgagcggcca tcatcatcag gtcggccaac tcgtcgatga
    13081 cgatgacgat gtatggcatg ccttcttcct tcttggcggc gttgtattca gcgatgttgc
    13141 tggtattgat ttcggccatg gtgcgcaagc ggcgctccat ttcggccacc gcccacttga
    13201 gggcgcttat caccttctcg ggctcggtaa tcaccggtgt cagcaggtgc ggaatgtcgt
    13261 tgtaggtccc gagttcgacg aatttcggat cgaccatgat gagcttgagg tcggccggac
    13321 tgttgcggta cagcaaactg gtcaggatgg tattgatcat gacggattta ccggaaccgg
    13381 tctgaccggc gaccaaaagg tgcggcatct tggccaggtc ggcgaccacc ggtgtgccgg
    13441 aaatgtcacg gccgatggca aagccgagcg gtccggatat gtcgctccac tgtttgctgg
    13501 tgagcagcgc gctcaaacgc accgttgccg gcttgacgtt cggcacctcg atacccacgg
    13561 cgcgcttgcc cggaattggt gcttccatac ggatactcgt ggctgccagg tcgagcgcca
    13621 ggttgttctc cagcgccgtg atcttctgca gcttcacgcc cgtcggcggt ttcatagtgt
    13681 actgggtgat gcgcggaccg acgttggcgc cttccatctc gacatcgatg ttgaagttgg
    13741 cgtacgtgtc atggataaga cgcgcgttgt gctccacatt gcccggatcg gccttgtcct
    13801 gcttctgatt cagccgcgta aggctcggga actgccagtc cgggtcgctg gcggccgtca
    13861 gcgcggcgtg gttttcgctg ggcgtcagct tctgggcggt gttgcggaac gatgccagtt
    13921 tgacgggttc cttggccggt tcttcgctgt gatgctccac cggcacgcct tcgttcagtt
    13981 tgaagccgcc ggtagctttg gctttgagct gggccagatc ggtgatttct tcgccctccg
    14041 gacgttcgcg gcgacggaat gaatgctcga acggccgcag taggaccttc ggggatgccc
    14101 cgagcgcgaa gcagatggcc agcagcgtca gcagcaggaa cagcaggctg gccggcagct
    14161 tgtcgagcat accgagtaca ccctgggcaa acgcattgcc gatggctccg ccgtgtacgc
    14221 cggtgaccgc tttgccacca gcatcatcag cggcgacgtg ggcgaacatc gtcccgaaga
    14281 aggcagccgc gaacaccagt gtgcagacca cactacttag cttagcgacc ggcaggcgac
    14341 gctcgtcgct ccgtaattta taaatgcccc aatacgccag cgcgaaggga gtcagcgccg
    14401 ccgcccagcc aaaggtggca taggcaccgt gatacatacc cttcggcagc gtgccgccgg
    14461 tgccgaaacc gccgagcagc aggaataatg ctaataagat gaggatgaag gcgcccgcca
    14521 acggccagaa caccgacggt tccttgggag gtggtgcagc gactttcttt tttgaggact
    14581 ttttcttttt tgccataact ggtttgatta tataattttc ggcctgcttg gtctaccctt
    14641 ctgggcgctc taatgcatcg aggttttgcc gcgcgcgacg aagccgctca gacgcttggt
    14701 tgagctcttc ggggtcaacg ggctcatccc ctcgcaggtt cggatcgata agtcgccggt
    14761 gagccaataa tgcctcgacc cattcagcct gcgcccgctc cgtcgtctcg gcagagggct
    14821 gcacacttac ttcatagagg cgaagcagtg catctcttac caactctcgg aagggctgca
    14881 attcttcctc ctcagctgtc tcgacgaact ggcgcagcgc gtcagcggcc tgttcggatt
    14941 ccaggtgtat aaactcttca ttaacagctt ccccctgctg agttcctggc tcctgcgagg
    15001 gttctgtgga cggtgattct tctggtggca tgccttcgtt atgtgacatg catcatatta
    15061 tagcaaaaaa tacaactgta tgtcaatgct tatgcttacc agccgccgcg gcgcatttta
    15121 cgatcaaggg cctgccgggc cagctgatgc gccccaaggc ggcgggcggg atcggtgtat
    15181 tgccggacaa agtcatcgtg ccgtctcgca agttcagggg gcagcggcgg caatgactct
    15241 tcctctacgt tgtcagctac ctctataact ggctctgaca actggttgat atctttaggt
    15301 gcttcaacgg tcatcgcggt tttttccgag cacggcgaag accctggagg aagttgggtc
    15361 ggccttcatc tgcgctcacc ttaaagatca tccactgctc atcagatatt ttgtcgctgt
    15421 tgcgctccgt tacgccgtcc tcgacatgat tcctaggcaa cagcggccgg gtcacccgag
    15481 gcgtcacttc cgtgttggtg gtttgagaaa tgctctcatg tctggcgagg ccgaatatat
    15541 tcccctgcgg accggtttct cgctcaagat cgtcatggaa tctctcggcc atttcggcga
    15601 tatcatcact ttcggtgcgc ggatacgtta ctgtagcatc cagagggtgt ccgttccaat
    15661 cgtgactcat ggggtgagct gcttcagaaa tcgttttcat aaccagatat aaacattagc
    15721 aagttattgc ttttttgtca atgtggttac gcttgtacta atcgacgcgg gcgtcctgcg
    15781 tcagcaggcg ttcgcgcatc tgctggaagc gcttctgctg ctccagggcg atttcctcgg
    15841 cggtcttggt acggccgccg gagacggtct tggcctcgtt gccgcccttg ccaccgccga
    15901 tgacatttac caccgggatg atgatcgggc tgcgctgggt ctgctcgaac aagtaatgcg
    15961 tgatgtggtc cttcaactct gccttgaagc ggtccaggtc gacgcgcttg aagcgctgag
    16021 taacggcacg gcgcagttcg ttgcgcaggc cgttcatgat ttcttcgttg tcgcgcatgt
    16081 atatgaaacc gcggctgacg atgtccgggc tggtcatgag gttgccggac ttcttgtcga
    16141 tggtcaggat gaccgccacc aggccttcct cggccagcaa gacacggtcc ttgaccacca
    16201 cgttgctgac aatggcaccg gtctgatcga ccaggatggt gccgtgcggt acttcgccga
    16261 tgacttccat cttgtcggga gtgagggcaa tgacctcacc gttcagagca ttgacacaat
    16321 tggcgcgcgg tacgccctgc tcgacggcaa gatcgatatg ccgctgtttg acgcggtacg
    16381 ctccgtagat cggaatgaag aacttcggct tggtgtactg gatcatttcg gcgtattcgt
    16441 cctggctggc gtgaccggaa acgtgcagcg gaccgacatg gtcgaggtcg tggttgcggg
    16501 cctcgaagac gtgcacgttc ttcatgacca tgttgtcgac catggtaccg accaacgcgt
    16561 cgttaccgct ttcaggaatc ggcgtggagc tgaggatgac cgtgtcctga gctttcagtt
    16621 tgatgtggcg gtgctcgccg ttggccatgc gctgcagcgc actgctcggc tcgccttggc
    16681 taccggtaca gaccacaacg agttggtcgt ccggcatggt ggcggcgctg gcaatcggca
    16741 cgaacgtccc tttcgggacc ttcatgaagc cgtggcgcac ggccatctcg agcgtgctga
    16801 ccatgctgcg gccgtccatg gcgatcttgc ggttgtggtg gatggcggcg ttgatgatca
    16861 tctgcacgcg attcatgttg gtggagaaca ggcctacgaa gatgcggccg ggtgcctggt
    16921 ccatgatgtc gatgaagctc tgctctatgg tgctttccga cggcgtgcga ccgagacgtt
    16981 cgacggtggt cgactcgctc aaaagcgcca ggacgccttc gttaccgagt tccttgagcc
    17041 gttcgatgtc acttcgttcg tggtcgagcg ggttcgggtc gaaccggaag tcaccggtgt
    17101 tgatgatgcg ccccaccggc gtgtccagca ccacgcaggt gctgcccgga atggagtgtg
    17161 ttatacgcac cagctcgacg aagaactcgc cgactttgag gcgttcgtgc gtatcttcgt
    17221 tcatagtgac agtcttgagt tcgaagccgt ccggcatcgg cagaccgaag ttctcgaaga
    17281 tttcctcgac ccggccgatg gtgaaccggc tgccgtagat aggtgccggg aatttcggca
    17341 cgatgtgcgg caggccgccg atgtgatcca ggtgtccgtg agtaatcaca tacgcccgca
    17401 gcttatgacg tatggtctcg aggtaggccg tatcggctac gccgtagttg atgcccggca
    17461 ggtcgacgct caggtcgtta ccggcgtcaa tcacgatagc gtcgttcaca tactctacga
    17521 gcatcatgtt cttgctaccg ccaccgtcca tgccgccgag accgataatc ttcaggcgcg
    17581 ggctgtcgtc gatgaagtta gcccgcttct gctcgggtga gacctgggcc gcggtgtact
    17641 ggttggcgat gcgctgcgcg tccatctgag agcgcttctg cgcccggacg gccgccccac
    17701 ggctgacgcc gctgcggcgc tcgccgccgt tattctggtc cttcggctgg ttctgccggt
    17761 tctggccgcc atggttgtta ccgccgcgac cccggccgtt cgccgccggt ctcccattct
    17821 gaccgccatt accacggcga ttgttctgag ccgggcgctg gccttgcggc cgcctgccct
    17881 gttgctcttg gttgttatcg ttattacttg gttccatagt tctccttttt ctaaaagttc
    17941 aatctattta atttctaatt cgtattctta tgcttcaaca tccaccggag acttgcggca
    18001 gtaaatggca gcaggcacag cataaacaca aaggccgcta ccgtgagcag ctggcctaaa
    18061 aggccagatc cgccgaatcc gtatgtcaca cgatgactga aagcttccgg tgccaatctg
    18121 aagatgaaca tataaattac cactacttcg gccactagct ctatgacatt gactacgata
    18181 tacaacagct tcatgggatt gctgctctgg agatattcgt cccctttcag gaactttctc
    18241 gtcatacgat tgctctgcac ccaggccact gtttcgtccg ctctaaaatc agcacgcagg
    18301 aacttccgct gcagcccttg gatgaacgtc tctttttgca ttacttattt tccttaattt
    18361 tctgaatgat taccgggata gccgcgaagt catcgatgag cgtttggcgc atgccgccgt
    18421 tgtagagtgc cgctgccggg tggaacagcg gcaggatgac gacttccagt acgtcgcctg
    18481 cgtgctcctt cagttgcagc ttcacccgtt taggttggcc gtggacctgt gaaatctgta
    18541 ggtccggcag gaagcaattc atgctgtgac gaccgagcgt caccaccact ttgggttgaa
    18601 ttatttccag ctggctctgc agatacggca gaaaggcttt cttttcgtcc ggcaacggat
    18661 cgcggttgtt cggcggccgg tatttgacga tgttggtgat gtaaatgtcc ttccgctcta
    18721 gcccgatgcc ttccagcatc tcattcagga acttccccgc cgccccgacg aacggcttgc
    18781 cctgcaggtc ctcgttcttg cccggtgcct cgcctataaa gacgatgtcg gcactcgggt
    18841 tgccgtcacc gaacaccaac tgcgtggcct gcgcctgcag ctgcggcgtc acctcatcgt
    18901 cgacgatctt ctgtgccagc tcgtccagcg cctgctgcct ggtcgtagta ctcatgaacg
    18961 cactactttc caaataagct gta
//
LOCUS       DASSFD010000150         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1341862, whole genome shotgun sequence.
ACCESSION   DASSFD010000150 DASSFD010000000
VERSION     DASSFD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341862"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /gene="fusA"
                     /locus_tag="VFH39_03655"
     CDS             complement(<1..1847)
                     /gene="fusA"
                     /locus_tag="VFH39_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6924897.1"
                     /translation="MADKKYPIGKIRNLGIIAHIDAGKTTTTEGILYRTGLKHKIGAV
                     HEGETTTDWMAQERERGITIVAASVTCFWKDHQINIIDTPGHIDFTAEVERSLRVLDG
                     AVVVFDGKMGVESQSETVWRQADKYGTPRICFINKINQTGGDFYKSLDTIHARLSKRA
                     FPIHLPIGFEQSVNGVVDLVSMTAYTYTNFADHELVEGEIPADMLDQAKKYRSLLIEN
                     AVAEDEAMLEKYFEVGEEGLTEDEIRHAIRSAVLTGKFYVVTGGDGRGVIVEKLLDLV
                     NDLLPAPNDRPTTWGVHPKTGDEIERKHESSDPFSALAFKITSDPFVGKLAYFRVYSG
                     KITAGSYVLNSSTGEKERVGRIVRLQADKREDVTEVDAGDIAAIVGLKGTTTGNTLCD
                     PNNPIILENITFPDPPVSIAIEPKTKADQEKMALALQRLAEEDPTFRVRTDEETGQTV
                     ISGMGELHLEIIVDRMKREFSVEANIGAPQVAYRESVRKSGVEVQGKFVRQSGGRGQY
                     GDVWLRISQNEQGAGFEFINSIKGGVVPGEYIKPVQQGIEEAMANGVIAGYPVVDIKA
                     ELYDGSYHEVDSSEMAFKIAGSMALQEGVKKAGPVLLEPVMKVVVVTPE"
     gene            complement(2297..2776)
                     /gene="rpsG"
                     /locus_tag="VFH39_03660"
     CDS             complement(2297..2776)
                     /gene="rpsG"
                     /locus_tag="VFH39_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET6924898.1"
                     /translation="MPRHKTKTLVRDIPGDRVYNDVRVQRLINRVMLNGKKQLAERLV
                     YSGMELAAKRLKVDNPIEVFEQAWKNVQPNVETRSRRVGGANYQIPFEVHGQRQSHLT
                     LMWFVAACRARKGISMAERIAAELQDAYNGQGAAVKKREDTHKMAEANRAFAHFARG"
     gene            complement(2779..3192)
                     /gene="rpsL"
                     /locus_tag="VFH39_03665"
     CDS             complement(2779..3192)
                     /gene="rpsL"
                     /locus_tag="VFH39_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET6924899.1"
                     /translation="MPTINQLIRKPRAKAHDKSKSPALQRVTNNLKTKNYEKPSPFKR
                     GVCVKVTTKTPKKPNSALRKVARVRLQNGYEVWAYIGGEGHNLQEHAVVMVRGGRVKD
                     LPGVRYHIVRGALDLQGVNNRKKARSKYGTKKEGK"
BASE COUNT          603 a          959 c          979 g          795 t
ORIGIN      
        1 tcttcgggcg ttacgacgac caccttcatg accggctcaa gcaagaccgg accggccttc
       61 ttgacgccct cttgcagcgc catggaaccg gcgatcttga aggccatttc ggaggagtcg
      121 acttcgtggt agctaccgtc gtacagttcg gctttgatgt ctactaccgg gtagccggcg
      181 ataacaccgt tggccatggc ttcttcgata ccttgctgga ccggtttgat gtactcgccg
      241 ggcaccacac cacctttgat ggagttgatg aactcgaagc cggcaccctg ttcgttctgc
      301 gagatacgca gccagacgtc accgtattga ccacggccac cggactgacg gacgaatttg
      361 ccctggactt cgacgccgct cttgcggacg ctttcgcggt aggccacctg cggcgcgccg
      421 atgttggctt cgaccgagaa ttcgcgcttc atacggtcga caatgatttc caggtgcagc
      481 tcacccatac cggagatgac ggtctggccg gtctcttcgt cggtacgtac acggaaggtc
      541 gggtcttctt cggccagacg ctggagggct agagccatct tttcctggtc ggccttggtt
      601 ttcggctcga tggcaataga caccggcggg tccgggaagg tgatgttctc aagaatgatc
      661 gggttgtttg gatcacacag cgtgttaccg gtggtagtgc ccttcaggcc gacgatggcg
      721 gcaatgtcgc cggcgtcgac ttcggtcacg tcctcacgct tgtcggcctg cagccggacg
      781 atgcgcccga cgcgctcttt ttcgccggtg gagctgttca acacatagct gccggcggta
      841 atcttaccgg agtacacgcg gaagtacgcc agcttaccga cgaacgggtc gctggtaatc
      901 ttgaacgcca gggcgctgaa cgggtcgctg gactcgtgct tgcgctcaat ctcgtcgccg
      961 gtcttggggt gcacgcccca ggtcgtcggc cggtcgttcg gagccggcag caggtcgttc
     1021 accaggtcga gcagtttctc gacgatgacg cctcggccgt caccaccggt gacgacgtag
     1081 aatttaccgg tcagcacggc ggagcggatg gcgtggcgga tctcgtcctc ggtcaggcct
     1141 tcctcaccga cttcgaagta cttctcgagc atggcttcgt cttcggcgac ggcgttctcg
     1201 atgagcaagg accggtactt tttggcttgg tcgagcatat cggccggaat ctcgccttcg
     1261 acgagctcgt ggtcggcaaa gttagtgtag gtgtaggcgg tcatgcttac caggtcaacc
     1321 acgccgttca cggactgttc aaagccgatg ggcaggtgga tcgggaaggc gcgcttgctc
     1381 agccgggcat ggatggtatc gagcgacttg tagaagtcac cgccggtctg gttgattttg
     1441 ttgatgaaac agatacgcgg cgtgccgtac ttgtcggctt ggcgccagac ggtctcggac
     1501 tggctttcca cacccatctt gccgtcgaag acaacgacgg cgccgtcgag cacgcgcaaa
     1561 gaacgttcga cctcggcagt aaagtcgatg tgtccagggg tgtcgatgat gttgatctgg
     1621 tgatctttcc agaaacaggt caccgaggca gccacgatgg tgataccgcg ttcgcgctcc
     1681 tgagccatcc agtcggtggt cgtttcgccc tcatggacgg caccgatctt gtgcttcagg
     1741 ccggtacggt acaggatacc ttcggtcgtg gtggtcttac cggcgtcgat gtgcgcgata
     1801 attcctaggt ttctaatctt tccaatcgga tacttcttgt ctgccatata ttccttctaa
     1861 gatttaatgc tcattaatca catcatgtat tgccaagcgt ccgactgaac ctcgacataa
     1921 ggcatgcctg cccggtattt aaagcctcca catcgtgtgg tacgtacggt ctctgtggtg
     1981 tgcctaaata gattcttaat tcttcgccgc ataacttcct gttcgtctac aatgggctgc
     2041 gagcaattgc ggaccccttc aatggctttt tggcctgtca gaccaagcat ggtgttatcc
     2101 catcggttcc aagtctcggc ggtgtctatt ccgctgattg tacgtgaacc atctccggcc
     2161 tcatcagaaa agctaaacac gaacctggtg tctcgcatac gcacttcttc atagtcaagt
     2221 gtatctgcta ccgaaatccc gcaggcgaga gggcaaccgt cacaaaactc cacaggtacg
     2281 ttgatgtttc ccatcattag cccctggcga agtgggcgaa ggcgcggttg gcttcggcca
     2341 tcttgtgggt gtcttcgcgc ttcttaaccg cggcaccctg accgttgtag gcgtcctgca
     2401 gctcggcggc gatgcgctca gccatgctga tacccttgcg ggcgcggcag gcagccacga
     2461 accacatcag ggtaaggtgg ctctggcgct ggccatggac ttcaaacgga atctggtagt
     2521 tggcaccacc gacacggcga gagcgggtct cgacgttcgg ctggacgttc ttccatgcct
     2581 gctcgaacac ctcgatgggg ttgtcgacct tcaggcgctt ggcagccagt tccatgccgc
     2641 tgtacaccag gcgctccgcc agctgcttct tgccgtttaa cataacgcgg ttgatgagcc
     2701 gctgcacgcg gacgtcgttg tacacgcggt cgcccgggat gtcccggact aaggtcttgg
     2761 tcttgtgcct aggcatgact acttgccctc cttcttggtg ccgtacttgg agcgggcttt
     2821 cttgcggttg ttgacaccct ggaggtcaag ggcgccacgg acgatgtggt agcgcacacc
     2881 cggcaagtcc ttcacacggc caccacggac catgacgacg gcgtgctcct gcaggttgtg
     2941 gccctcgcca ccgatgtaag cccagacttc ataaccgttc tgcaaacgga cacgggcgac
     3001 cttacgaagg gccgagttcg gcttcttcgg ggtcttggtg gtgaccttta cgcagacacc
     3061 gcgcttgaac ggcgacggct tctcgtagtt cttggtcttg aggttgttgg tgacacgctg
     3121 cagggccggc gacttagact tgtcgtgggc cttggcacgc ggcttcctga taagttggtt
     3181 gatagttggc atagtactcc ttgtggttct gcactctatc cgcggcgatc gagtatgccg
     3241 gctcgaaggg ctcggagtgc ttatataact tgtaaattct accagataag ctggctcttg
     3301 acaacgggtg taatacaccg cctcgtcaag acccct
//
LOCUS       DASSFD010000151         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_944994, whole genome shotgun sequence.
ACCESSION   DASSFD010000151 DASSFD010000000
VERSION     DASSFD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944994"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="glyA"
                     /locus_tag="VFH39_03670"
     CDS             <1..771
                     /gene="glyA"
                     /locus_tag="VFH39_03670"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641461.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET6924900.1"
                     /translation="AKFAAIAREVGDQCLLMADMAHIAGLIAGGVAKNPFDFGFHVIT
                     TTTHKTLRGPRGGLILSKGTVSSPLKAPEKTLANIPTLIDRAVFPGMQGGPHEHIIAA
                     KAVAFGEALKPDFKQYAQQIVKNAAALADALQARGFKLITGGTSNHLILADVYGSFGI
                     DGKVVEEALDKIGLTLNKNAIADDPLPPFKPSGIRLGTPAITTRGLVEKDMEQIAEWM
                     KQAIDNREDEAKLNLLRNEVRDFALQYPLPSDKTTSKE"
     gene            1012..1467
                     /locus_tag="VFH39_03675"
     CDS             1012..1467
                     /locus_tag="VFH39_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6924901.1"
                     /translation="MRQRFTVVPAAYVIMRRGKEVLLIRRAYTGYFDGYYSFPSGHID
                     GGESAKTAAIREAKEEVGVVIKPEDLRFVHVMHRIAEEGDHERVDFAFETTEWQGEPT
                     NVEPDKCDDVQWFPLDALPDTIVPLAREMLEHVAAGSYYSDHRFETPAA"
     gene            complement(1464..1715)
                     /locus_tag="VFH39_03680"
     CDS             complement(1464..1715)
                     /locus_tag="VFH39_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924902.1"
                     /translation="MAKKQKHTRRAKRKNVWFIKVRGSYLPSSWQGWLTYIPFTAYLL
                     WSLSVGWQGTHTLGMALLFIVPNWIAAAAVTTWVAKHTS"
     gene            1779..2720
                     /locus_tag="VFH39_03685"
     CDS             1779..2720
                     /locus_tag="VFH39_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924903.1"
                     /translation="METTRLNLRPLCVLGVAGLLGLLGLLQLGMATRNLTQPVAYRRR
                     HAPRPYTFPDGGRTLFPDYRFVALYGSLQYPELGALGEQSMDQSIARAKALAARYQPL
                     VSEHVMPAFEIIATVASDSPTEDGDYSAALNPAQIQPWITAARQNDMYVVLDLQPGRS
                     DFLTQAKLYTSLLKLPNVGLALDPEWRLKPDQVPLAQIGSVSIDEVNRTLTWLSDLTA
                     KGHLPQKLVVLHEFRSDMLPDRQNLDTSHANLAYVIQMDGSGPPAGKQDTWRGITANP
                     PANVSFGWKNFYHQDTPMIDPAATMAIRPQPRYVSYQ"
BASE COUNT          611 a          778 c          832 g          553 t
ORIGIN      
        1 gccaagttcg cggccatcgc ccgcgaagtc ggtgaccagt gcctgctgat ggccgacatg
       61 gcacacatcg ccgggcttat cgccggtggt gtcgccaaga acccattcga ttttggtttc
      121 catgtcataa ccaccaccac gcacaagacg ctgcgcgggc cgcgcggcgg cctgattttg
      181 tcgaagggca cggtcagtag tcccctcaag gcacccgaaa agacgcttgc caacatcccg
      241 acgctcatcg atcgggcggt gttcccgggc atgcagggcg gcccgcacga gcacatcatc
      301 gccgccaaag ccgtcgcttt cggcgaagca ctgaagccgg atttcaagca atacgcgcag
      361 cagatcgtga agaacgcggc cgcgctggcc gacgcgctgc aagcccgtgg cttcaagctt
      421 atcaccggcg gtaccagcaa ccacctcata ctggcggacg tgtacggcag cttcggcatc
      481 gacgggaaag tggtggagga agcgttggat aagatcggtc tgacgctcaa taagaacgcc
      541 atcgccgacg atccgttgcc gccgttcaag ccgagcggca tccgcctagg gacgccggcc
      601 atcaccactc gcggcctggt cgaaaaagac atggagcaga ttgccgaatg gatgaagcag
      661 gcgatcgata accgcgaaga cgaagcaaag cttaacctgc taaggaacga agttcgcgat
      721 ttcgcgttgc agtacccgtt accgagtgat aaaactactt caaaggagta atcatgacag
      781 agtttggccc caggtctgag attgagggag cggaggacga actggtgagc atgccaaacg
      841 tacccgttga gtacccgcgg cagcgagaaa tgacattccc cgtgttagat gcgagcgggg
      901 cgtgggcgga caaaacggtt tacgtttttg agacgccaac gataggtgaa tcaggcgtgc
      961 ttaccatgct caggagaaac ttcccgccac ctaaccctga gaaataagtt gatgagacag
     1021 cgttttacgg ttgtgccagc ggcatacgtt atcatgcggc ggggcaaaga agtattgctg
     1081 atccgccgcg cctataccgg ctacttcgat ggctactact cgttcccgtc cggccatatc
     1141 gatgggggcg agtcggccaa aaccgccgcc atccgggagg cgaaagaaga agttggcgtg
     1201 gtcataaaac ccgaggattt acgcttcgtg cacgttatgc accggattgc cgaggaaggt
     1261 gatcacgagc gggtggactt tgctttcgaa acaactgaat ggcaaggtga gccgacgaat
     1321 gtcgagcctg ataaatgtga cgatgtgcag tggttcccgc tcgatgcctt accggatacc
     1381 atcgttccgc tggcacggga aatgttggag cacgtcgcag caggcagcta ttactccgac
     1441 cacaggttcg aaaccccagc cgcttagctg gtgtgtttgg cgacccaggt cgtgacggca
     1501 gcggccgcaa tccagttggg gactatgaac aacagggcca tgcctagcgt atgcgtgccc
     1561 tgccagccaa ccgatagcga ccacagcagg taagcggtga agggaatgta ggtgagccag
     1621 ccctgccagc tgctggggag gtagctgccg cggaccttta tgaaccagac gtttttgcgc
     1681 ttggcgcgac gggtatgttt ttgctttttg gccatcaact ttagtgtagc atggccgacc
     1741 gctacgctgg tcccgacata tgcggtatgc tggatgggat ggaaacgacg cgactgaacc
     1801 tgaggccgct gtgcgtattg ggggttgccg ggctgttggg attgctcggc ttgctgcaac
     1861 tgggcatggc tacgcgtaat ctgacgcaac ccgttgccta ccggcgccgg cacgcgcccc
     1921 ggccgtacac cttcccagac ggtggcagaa ccctcttccc ggactaccgg ttcgtggcgc
     1981 tgtacggctc tttacagtac ccggagctcg gtgcgcttgg ggagcagtca atggatcaat
     2041 cgatcgcccg ggccaaagca ttggcggccc ggtatcagcc gttggtatca gaacacgtga
     2101 tgccggcgtt cgaaatcatc gcgacggtgg cttctgattc gccgaccgaa gatggtgatt
     2161 attctgcggc tctcaatcca gcgcagatcc agccgtggat tacagcggca cgccagaacg
     2221 acatgtacgt ggtgctcgat ctgcagcccg ggcgcagtga ttttttgacc caggctaagc
     2281 tgtatacctc actccttaaa ctgcctaacg tcggactggc gctggatccg gagtggcggc
     2341 tcaagcctga tcaggtcccg cttgcgcaga tcggttcggt gagcatcgat gaggtcaacc
     2401 gtacgctaac ttggttatct gacctgactg ccaagggcca cctgccccag aaattagtgg
     2461 tgctccatga gttccggagc gatatgctgc cagaccgtca gaatttagat acttcgcatg
     2521 ctaaccttgc ctacgtgata cagatggacg gcagcgggcc accggctggc aagcaggata
     2581 cctggcgagg tataaccgct aacccgccgg ccaacgtatc gtttggctgg aagaactttt
     2641 accatcagga tacgccgatg atcgatcctg ctgccaccat ggccatccgc ccgcaaccgc
     2701 gctacgtgtc ctaccaataa caggggtacg cattatcaaa tttgcagtga tgtggtgtaa
     2761 tggaaggtat gaag
//
LOCUS       DASSFD010000152         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_821491, whole genome shotgun sequence.
ACCESSION   DASSFD010000152 DASSFD010000000
VERSION     DASSFD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821491"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFH39_03690"
     CDS             complement(<1..320)
                     /locus_tag="VFH39_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924904.1"
                     /translation="MTELDPELRCESENTFLDPADQAALLEEAQELELVDERTLRDHF
                     LHPDTKDLATAGSVVGGLLAIYFAARALRRRDGKISMPDGDVSLHLEPAEYMKGFVSY
                     VTKKG"
     gene            complement(337..1032)
                     /gene="recO"
                     /locus_tag="VFH39_03695"
     CDS             complement(337..1032)
                     /gene="recO"
                     /locus_tag="VFH39_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET6924905.1"
                     /translation="MKQLLTTGIILRRTDYGEADRIILLLTPEQGKLSLVAKGVRRAK
                     SKLAGGIELFSVSHITYLPGRSGSGLGTLVSTRLIRHYGHIVQDITRTMLGYDLIKQL
                     NKVTEDNPEPDYFELLEQTFAALDDQTVPPELIKIWFNAQLIRLGGHTPNLQTDTAGD
                     KLAADSRYDFDYDQMAFARHADGNFGVDQIKFLRLIFSGNQPAVLSKVTGVTEVTKQV
                     SPLVTLLSQQYIS"
     gene            complement(1058..1462)
                     /locus_tag="VFH39_03700"
     CDS             complement(1058..1462)
                     /locus_tag="VFH39_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6924906.1"
                     /translation="MKILLVEPDATLARIYRLALEHDAHQVRTAATAQEGIQVADAWR
                     PELVILELQLVSHSGIEFLYEFRSYDDWQSIPVIIHSFIPPAEFVASRVLCDELGIAD
                     FLYKPRTSLQQLRRRVGQNVLSEAGNTAVIAA"
     gene            complement(1459..2178)
                     /locus_tag="VFH39_03705"
     CDS             complement(1459..2178)
                     /locus_tag="VFH39_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924907.1"
                     /translation="MGRNRTGQAALSEQLLLAVAEELKLPLLQVSRMAEYGRLEDSTA
                     TLADIQTTADAALGLLDTYLFGARLALDGDYGLQLEPVSVSSVLYDAGQQLDRLAKLY
                     GVELELSISGRFGTVMAHRGGLQSALTSLGYALIEALPNLQNGRLTLQLGAHRCRYGI
                     VAGVYSEAEQLTTEALRAGRKLQGHSRQPLPTLTHGGGAGVFVADAILQAMDSQLTPT
                     RHHRLYGLGTVLKPNSQLQLV"
     gene            complement(2270..2578)
                     /locus_tag="VFH39_03710"
     CDS             complement(2270..2578)
                     /locus_tag="VFH39_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924908.1"
                     /translation="MPGTSDQSSTFALRTQVVGQAYSDVLQNINNTQGDKVTPYSLPK
                     LPKIVGVRVDGPLLNDQTKSGSTIILPLRDKTLLVWTESSQFMGDFNNYILPNLTFSP
                     "
     gene            complement(2654..2929)
                     /locus_tag="VFH39_03715"
     CDS             complement(2654..2929)
                     /locus_tag="VFH39_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924909.1"
                     /translation="MVNRNQSGAINVLLLPLIIAVLLLIAAGSFGYWAYSGRQDYKNN
                     VDAKIAVAVANAVKAEDVKKAAEYVEASKQPLRTYVGPEQYGALRVM"
     gene            complement(2992..3237)
                     /locus_tag="VFH39_03720"
     CDS             complement(2992..3237)
                     /locus_tag="VFH39_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HET6924910.1"
                     /translation="MEIIGYLAGLLTLVGYLPQTIKTIRTRKTRDLSLPTFLIIGTSA
                     VLWTVYGLHTHTPSIWVTNGVVTACSLIILVLKVRED"
     gene            complement(3261..3749)
                     /locus_tag="VFH39_03725"
     CDS             complement(3261..3749)
                     /locus_tag="VFH39_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924911.1"
                     /translation="MTIIGLILVALIFCFGFVLLFGAPYLPTLTKQMQVALDLVDLQP
                     GERLVELGCGDGKVLVAAARRGWLVDGYELNPLLVLVCVWRTRHFRKQVRVHWRNFWT
                     AQLPPTEGIFVFVLQRQMAKLDNKIVQEVSKPVKLVSFAFTIPGRKPVRSKEGVYLYE
                     YR"
     gene            complement(3757..3960)
                     /gene="xseB"
                     /locus_tag="VFH39_03730"
     CDS             complement(3757..3960)
                     /gene="xseB"
                     /locus_tag="VFH39_03730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6924912.1"
                     /translation="MTAKKLDYQGLSDELETVLLTLQQEDVDVDTAMAAYERGLKLVG
                     QLEAYLSDAENTVKKLQAASDNG"
BASE COUNT          785 a         1190 c         1138 g          886 t
ORIGIN      
        1 cccttcttcg tcacataact cacgaagcct ttcatgtatt cggcaggttc aagatgcagc
       61 gacacatccc catcaggcat actgattttg ccgtcgcgtc gtcgcaacgc ccgggcggcg
      121 aaataaatcg ccaataagcc tcctaccacc gagccggcag tagccagatc tttcgtatcc
      181 ggatgcaaga agtggtcgcg caacgtgcgc tcatctacca gttccagctc ttgagcctct
      241 tccagcagcg ccgcctggtc ggccgggtca agaaaagtgt tttctgactc gcatcgaagt
      301 tcaggatcca attcagtcat cgtttccccc ttagtactac gaaatatact gctggctcag
      361 caaagttaca agagggctca cttgtttggt cacttcggtt acaccggtga ctttggagag
      421 gactgcaggt tggttgccgc tgaagatgag acgcaggaac tttatctggt cgacaccgaa
      481 attaccatcg gcatgccgcg cgaaggccat ctggtcgtag tcgaagtcgt agcggctgtc
      541 ggctgccagc ttatcgcctg ccgtgtccgt ttggaggttc ggcgtatggc cgccgagccg
      601 gataagctga gcgttgaacc atatttttat aagctccggc ggcacggttt ggtcatccaa
      661 ggccgcgaat gtctgttcca acagctcgaa gtagtccggc tcggggttat cctcggtgac
      721 cttgttgagc tgctttatga ggtcgtagcc gagcatagtg cgggttatgt cctgcacgat
      781 gtgcccatag tgccggatga gccgggtgga gaccagcgtg ccgagcccgc tgccgctcct
      841 ccccggcaga taggtgatgt ggctgacact gaacagctcg ataccgccgg ccagtttgct
      901 tttggcgcgg cgcacgccct tggcgacaag actcagtttg ccctgctcag gcgtcagcag
      961 caggatgata cggtcggcct cgccgtaatc ggtgcgccgc aggatgatgc cggtagtgag
     1021 cagctgtttc atgaatctat ctaagtcgtt atgttattca ggcggcgata actgccgtat
     1081 ttcctgcctc gctgagtaca ttttggccta ccctgcgccg tagttgctgg aggctggtcc
     1141 tcggtttgta caagaaatcc gcgatcccca gctcgtcgca caagacccgg ctggccacga
     1201 attccgccgg cggaatgaaa ctgtggatga tgaccgggat gctctgccaa tcgtcataac
     1261 tgcggaattc gtacaaaaat tcgatgccgc tgtggctgac gagctgcagc tccagaatga
     1321 ccagctcggg ccgccaggca tcggccactt gtatcccttc ctgggccgtc gctgcggtac
     1381 gcacttgatg ggcatcgtgt tccaacgcca gccgatagat tcgggcgagc gtggcgtcgg
     1441 gttcgaccaa caggatcttc ataccagttg cagctgcgag ttaggtttga gcaccgtacc
     1501 caggccatac aagcggtggt ggcgtgtggg cgtcagctgg ctatccatgg cctgcaggat
     1561 ggcgtcggcc acgaaaactc ccgccccgcc gccgtgggtc agcgtcggta acggctggcg
     1621 gctatggccc tgcagtttgc ggccggccct tagcgcctcg gtggtcagtt gctcggcttc
     1681 gctgtagacg ccggcgacga tgccgtaacg acagcggtgc gccccgagtt gtaacgtcag
     1741 gcgtccgttc tgtaagttcg gcaacgcttc gataagcgcg tagccgaggc tggtcagggc
     1801 tgactgcaac cctccgcggt gcgccatgac ggtgccgaag cggccgctga tgctcagctc
     1861 cagttccaca ccgtacagct tggccagccg gtccagctgc tggccggcgt cgtacagcac
     1921 gctgctcacc gataccggct ccagttgcag cccatagtcg ccgtccagtg ccaggcgtgc
     1981 cccgaacagg taggtatcga gcaggccgag cgccgcatcg gcggtggtct gaatgtcagc
     2041 cagggtggcc gtcgagtctt ccaatcggcc gtactccgcc atacgcgaca cctgcaacag
     2101 cggcagcttc agttcttcgg cgacggccag cagcagctgt tcggataacg ctgcctgacc
     2161 agtcctgttt cgccccatgc actgcccctt ttcttaccac ccagtatagc ccagtcaggc
     2221 ttgcaggacc aagccgatta gtagctgata cgcttatgct taagggtcgt tacggtgaga
     2281 acgtcaagtt gggcaggatg tagttgttga agtcgcccat gaattgcgag gactccgtcc
     2341 agacgagcaa tgtcttgtcg cgcagcggta gaatgatcgt actgccggat ttggtctggt
     2401 cgttcaacag cggcccgtcg acacggacgc cgacgatttt cggcaacttg ggaaggctgt
     2461 acggagtgac cttatctccc tgggtgttgt tgatgttctg gagtacgtca ctgtacgcct
     2521 ggcctaccac ctgggtgcgc agcgcgaagg tgctgctctg atcgctcgta cccggcaccg
     2581 tccccgggtc gtagtaaccg tctacggccg ggtcagactg agagttgtca acataggcgc
     2641 tccatgtccg cggctacatg acacgcaagg caccatactg ctcggggccg acatacgtcc
     2701 gcagaggctg cttcgaggct tcgacgtatt cggccgcctt cttgacgtct tcggccttga
     2761 ccgcattggc gacagccacg gcgattttag catcgacgtt attcttatag tcctgccggc
     2821 cactgtaggc ccaatagccg aaactaccgg cggctatcaa aagcagcacg gcgatgatga
     2881 gcggcagcag caatacattg attgcccctg actgatttct gttaaccatt accgtcatag
     2941 taccctagta gcgcttccct ttcaagcatg ccgtggggct cggatgcggc cttaatcctc
     3001 gcggactttc aggaccagaa taatgagact gcaagccgtc accaccccgt tcgtcaccca
     3061 gatggacggc gtgtgggtat gcaagccata gaccgtccac aaaaccgccg aggtgccgat
     3121 gatcaggaac gtgggcaatg acagatcgcg cgtcttacgg gtccggatgg tcttgatggt
     3181 ctgcggcaaa tagccgacca gcgtcagtag gccagccagg taaccgatga tttccatgct
     3241 catcagtata gcgcagtacc tcagcggtat tcgtacaggt agacgccttc tttgctacgc
     3301 accggctttc ggcctggaat cgtaaatgca aagctcacca atttgacagg tttgctaact
     3361 tcttgaacaa ttttattatc cagcttcgcc atttgccgct gtaatacgaa aacgaatatc
     3421 ccctctgttg gcggtagctg cgcagtccag aagttacgcc agtgaacacg gacttgcttg
     3481 cggaaatgac gcgtccgcca cacgcagacc agcaccagca gcgggttcag ttcgtagccg
     3541 tctacgagcc agccgcgccg tgctgccgcc accaacacct tcccgtcgcc gcatccgagc
     3601 tccaccagcc gctcgccggg ctgcagatcg accagatcga gggccacctg catctgtttg
     3661 gtcaacgtcg gcaagtacgg cgccccgaac agcagcacga agccgaagca gaatatcaat
     3721 gctaccagta tcagaccgat gatcgtcatg ggctatctag ccgttatcgc tggcggcctg
     3781 cagcttcttt acggtatttt ccgcgtccga caggtaggct tcgagctgcc cgacaagctt
     3841 gagcccccgc tcgtacgcag ccatggcggt gtcgacatcg acatcttcct gctgcaatgt
     3901 cagcagcacc gtttcgagct catcgcttaa gccttgataa tcaagtttct tggcagtcat
     3961 atgtctcctt ttacttcggc ggaccggcgg tcatcgtgg
//
LOCUS       DASSFD010000153         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_857463, whole genome shotgun sequence.
ACCESSION   DASSFD010000153 DASSFD010000000
VERSION     DASSFD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857463"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFH39_03735"
     CDS             <1..1191
                     /locus_tag="VFH39_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924913.1"
                     /translation="MNNTINLNSQSGNANVTSNTKGGNATSGTANAVANIVNTINSYV
                     TAGKSFVGVININGNLNGDILLPPNFIDTLLANNVPHYNITTNLNNQTQVTSVNNATV
                     NNNVNLASASGNATVTGNTAGGTATSGNALTNLTVFNLTGSNVVASNDMLVFVNVLGK
                     WYGMIMNAPAGSTAAELGGGVSANTSVNNNASLTNTNNQAINNDINVNAQSGDATVNR
                     NTQGGNAQSGNATASVNIMNFINDRLSLTGWFGLLFINVFGTWNGSFGINTAAGNPPL
                     AVPQSSGDSSRVTPMYTFVPKTVSGGNGGSYGSSFIGGGNGGSNGFYGTTTGSSSSTS
                     GSAATLAAKAGVLAATTQKAASQVPQLAKNSGANYLVPILGTVLAVILVIVGERGSLL
                     RRHS"
     gene            1218..1766
                     /locus_tag="VFH39_03740"
     CDS             1218..1766
                     /locus_tag="VFH39_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924914.1"
                     /translation="MRLLPDLIDTALLRQLLWRRLFVTLPLTLIAVFVLSAYTQLSGV
                     PSGSGAASSSSTHHQTTTVLQPDPANTPSVPDSQSTTAGPSVAYTPPVTATAPVTTPE
                     QPATDSTADSSTPADSQTPAIVAPSPSQTAAPAPTCTAAPSDNEPCGYTPPPVGSCGC
                     NPNYYDHLHPATMCPMYACAAY"
     gene            1958..2034
                     /locus_tag="VFH39_03745"
     tRNA            1958..2034
                     /locus_tag="VFH39_03745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1992..1994,aa:Ile,seq:gat)
     gene            2157..2233
                     /locus_tag="VFH39_03750"
     tRNA            2157..2233
                     /locus_tag="VFH39_03750"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2191..2193,aa:Ala,seq:tgc)
     gene            2487..2906
                     /locus_tag="VFH39_03755"
     CDS             2487..2906
                     /locus_tag="VFH39_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924915.1"
                     /translation="MTAGISPEIPADISAVDRTERARQLASSLRIAELETAAGTPVVD
                     YVADLLELSPLDLQLYHAWLFQDGVITYHRGHFRVNETISRADIEQQFPAYPGVKLLL
                     ENAMANNAPLAEQAATLLRGGYAVHRAPADTELPAST"
     gene            complement(2968..3336)
                     /locus_tag="VFH39_03760"
     CDS             complement(2968..3336)
                     /locus_tag="VFH39_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET6924916.1"
                     /translation="MEENNSAIRTMSGLNFIFGIWLIISPYILSYTTAQAKWEQTVAG
                     IVVLILAAIRYAAPKQMWTSWVNAAVGVWLIIAPFATGYQSGAAFWNEVIFGILVAIV
                     GISNGLTRGIGHTGHHHQAV"
     gene            complement(3444..3947)
                     /locus_tag="VFH39_03765"
     CDS             complement(3444..3947)
                     /locus_tag="VFH39_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924917.1"
                     /translation="MSKLFTPENSEQLVLSPVYPGKQQTAERLDQLLAEGSGWGPGAD
                     GWVSDHSPVWTASLRDNPVPVGYVYYGHHAREPRVMVYGGRTAEHLPNGEPLTHDIRA
                     NVSAALLHTALNDVRLPARLYVAPKVSLPGAVMENLNNRITPVQQVLGELALLHPWLA
                     LAETPAE"
     gene            complement(3975..4556)
                     /locus_tag="VFH39_03770"
     CDS             complement(3975..4556)
                     /locus_tag="VFH39_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924918.1"
                     /translation="MDIQKSYATVAFMYERMYVPEAGEPICVFPVGPHAGRRAHKLEK
                     YLLEDGTWLEGFLRGGQPARNYDHNEHDPTTRTSTVWEALDRSGGGEPRTMGFIQTQS
                     ARDAVCIRRGMITRRLDREKDRERRMDISAALFHAALHGRDSIDVQVRCRKMMLLDVP
                     PTIAGYTENVRNLLYVMREGRPWLERAEEHIHA"
BASE COUNT         1070 a         1542 c         1230 g          912 t
ORIGIN      
        1 atgaacaaca ccatcaacct gaactcgcag agcggcaacg ccaacgtcac cagtaacacc
       61 aagggcggca acgctaccag cggtaccgcc aacgccgtcg ccaacatcgt gaacaccatc
      121 aacagctatg tcacggccgg gaagtcgttc gtcggtgtca taaacatcaa tggcaacctg
      181 aacggtgaca tcctgctgcc cccgaacttc atcgacaccc tgctggccaa taacgtgccg
      241 cactacaaca tcaccaccaa cctgaacaac cagacccagg tgaccagcgt caacaacgcg
      301 accgtcaaca ataacgtcaa cctggcatcg gccagcggca acgccaccgt caccggcaac
      361 actgccggcg gtaccgccac cagcggtaac gcgctcacca acctgactgt cttcaacctg
      421 accggcagca acgtggtggc cagcaacgat atgctggtgt tcgtgaacgt gttaggcaaa
      481 tggtacggga tgatcatgaa cgcgccggcc ggctccacgg ccgccgaact gggcggtggc
      541 gtcagcgcca acaccagcgt gaacaacaac gccagcctga ccaatacaaa caatcaggcg
      601 ataaacaacg acatcaacgt caatgcgcag agcggcgacg ccaccgtcaa ccggaacacg
      661 cagggcggta acgcccagag cggtaatgcc actgccagcg tcaacatcat gaacttcatc
      721 aacgaccgct tgagcctcac cggctggttc ggcctgctgt tcatcaacgt cttcggcacc
      781 tggaacggca gcttcggtat caacaccgcc gccggcaacc cgccattggc cgtgccgcaa
      841 agcagcggtg acagcagccg tgtaacgccg atgtacacct tcgtccccaa gaccgtcagc
      901 ggcggtaacg gcggcagtta tggctcaagc tttatcggtg gcggcaacgg tggcagtaat
      961 ggcttctacg gtaccactac cggcagctca agcagtacga gcggcagcgc agccaccttg
     1021 gccgccaagg ccggcgtcct ggccgctacc actcagaaag ccgcttccca ggtaccccaa
     1081 ctcgccaaga acagcggtgc caactacctc gtccccattt tgggcaccgt cctggcagtg
     1141 attctggtaa tcgtcggcga gcgcggcagc ctgctccgcc gccactcata accccttaac
     1201 ccctgagagg atacgatatg cgtttactcc ccgatctcat cgataccgcc ctgctccgcc
     1261 agctgctatg gcgccgcctg ttcgtgacac tgccgctgac gctcatcgcc gtgttcgttc
     1321 tgagcgccta cacgcagcta tcgggcgtcc ccagcggctc tggggcggca tcatcgtcga
     1381 gtacgcacca ccagaccacg accgtactgc agccggatcc ggctaacact ccgagcgtac
     1441 cggacagcca atcgactacc gctggcccgt cggtagccta taccccgccc gtgaccgcaa
     1501 cggctccggt gacaacacct gaacaacctg cgaccgacag tactgcggat tccagtactc
     1561 ccgccgactc ccagacgccg gcgattgtcg ctccaagtcc ttcgcagacc gctgcaccgg
     1621 caccgacttg cacggccgcc ccaagcgata atgaaccctg tggctacacg ccgccgcccg
     1681 tcgggtcctg cggctgcaac cctaactact acgatcacct gcacccggcc accatgtgcc
     1741 cgatgtacgc ctgcgccgcc tactagccct acccttgtat aaatgccgct ttttcgctag
     1801 aatacttcgg taactttctc tcttcataaa caggtataag agagcggtca ccaatctttt
     1861 aaaatctatg attatgaaag attgagagga ggaccgtagc cgtgagtcga agcttttgcc
     1921 gcgaggtaaa acgagaccaa ggcgcacgga acgacgaggg gctatagctc agttggttag
     1981 agcgcgtcac tgataatgac gaggtctctg gttcgagtcc agatagcccc accaggaatc
     2041 atcgattcca ccccaattcg ttcgagactg cttgaatagg gaaaagagca gcccgagtcg
     2101 gcggattcat tccgccgccc ggcgcgatga gaaaaaccgc aggtttgtct cattccgggg
     2161 caatagctca gctggctaga gcacctgctt tgcaagcagg gggtccgggg ttcgaatccc
     2221 cgttgctcca ccaaataaaa cgtccggtcg taggccgggc tttttatttg gtgaggcatg
     2281 aggaccccga cgcgggttcg atagtcgagc gatcagcgag aatatatgcc gagcccgact
     2341 tgcccagccg ccaatgtaat cggcaagccg gcgcgaagct ataatcccca ttgctccacc
     2401 gcgcaaaacc acaacgttca taagcatttt cgtgattgac ttcccggcgc aacgtactac
     2461 agttagttca tgacagcgga ccggctatga ccgcgggcat ctcccccgaa ataccagcag
     2521 atatatctgc tgttgaccgc acggagaggg cccgccaact agcctccagc cttcgtatag
     2581 cagagctcga aacagccgcg ggcaccccgg tcgtcgacta tgtggctgac ctgctcgaac
     2641 tttccccgct cgatctgcaa ctttaccacg cctggctatt ccaagacggg gtcatcactt
     2701 atcaccgcgg gcatttccgc gtcaacgaaa ctatcagccg ggccgacata gaacagcagt
     2761 tccccgctta tccgggcgtc aagttactac tcgagaacgc catggcaaac aatgcaccat
     2821 tagccgaaca ggcggccacg ctcctgcggg gcggctacgc cgtgcaccga gcgccagccg
     2881 acacggaact gccggcaagc acatagaaaa agcccgctct tttacgtgag cgggctttcc
     2941 cttccggggg gtggacgaaa ttaaaggcta gacggcctgg tggtgatggc cggtgtggcc
     3001 aatgccgcgc gtcaggccat tgctgatgcc gacgatggcg accagaatgc cgaagatcac
     3061 ttcgttccag aaggcggcgc cagactggta cccggtggcg aacggggcga taatcagcca
     3121 tacaccgacg gctgcattca cccagctggt ccacatctgc ttcggtgcgg cgtagcggat
     3181 ggctgccaat atcaggacca cgataccggc gacagtctgt tcccacttag cctgggcggt
     3241 cgtgtagctc agaatgtacg gtgaaataat caaccaaata ccgaatataa agtttagccc
     3301 ggacatcgtc cgtatggcgg aattgttctc ttccataaag tacctcctac tttcctctta
     3361 cactttccac cttactccgt tgtgatacgc ctcactatta aaaaattcat agtagctagt
     3421 tcggcgagtg aagtgccaga gagttattcg gcaggtgttt cggccaaagc caaccagggg
     3481 tggagcaatg ctagctcacc caatacctgt tgcaccggag tgatacgatt attgagattt
     3541 tccatgaccg ccccaggcag ggataccttc ggcgccacat agagccgcgc cggcaagcgc
     3601 acgtcgttca gcgccgtatg cagaagggcc gccgacacgt ttgcccgtat gtcgtgggtc
     3661 aatggctcgc cattcggcag gtgctcggcc gtccgacccc cgtagaccat tacccgcggc
     3721 tcgcgggcgt gatgtccata atagacgtat ccgaccggaa cgggattatc acgcaggctg
     3781 gccgtccaga ccgggctatg atcgcttacc cacccgtctg cgccaggccc ccagccactg
     3841 ccttctgcca atagctggtc caggcgttcg gcggtctgct gcttaccggg gtagaccggg
     3901 ctcaggacga gttgttcgga gttctcaggg gtaaacagct tgctcatgct atgactctat
     3961 cacaacggca gcggctaagc atgaatgtgc tcctcggccc tctccagcca cggccggcct
     4021 tcacgcatca cgtacagcag gttccgcacg ttctccgtgt aacccgcgat tgtcgggggt
     4081 acatcgagca gcatcatttt cctgcacctc acctgcacat cgattgagtc gcgtccgtgc
     4141 agagctgcgt ggaacaatgc ggccgatatg tccatgcgcc gttcccggtc cttttcgcgg
     4201 tcgagccgcc gcgtaatcat gcctcgcctg atacatacgg catccctagc gctttgcgtc
     4261 tgtataaagc ccatcgtccg cggctcaccg ccgccgctgc ggtccaacgc ttcccacacc
     4321 gtgctggtcc tggtggtcgg atcgtgctcg ttgtgatcat aattccgcgc cggctggccg
     4381 ccgcgcagaa acccctccag ccacgtcccg tcctccagca gatacttctc aagtttatgc
     4441 gcccgccgcc cggcgtgcgg tcccaccgga aacacacaaa ttggttcccc agcctcgggc
     4501 acatacatcc gttcgtacat gaacgcaact gtagcatagc ttttttgtat atccataagc
     4561 gccctatttc cccgagaagc ttgaaagtaa gtggtttact acaaaggttg ataattcact
     4621 ctgttgcagc gaaacttgca gcaaagaaaa ccaatcccag caccagcata atgatgccca
     4681 gtataggcag ccaaaaaata gctactacag cgccaattaa tgccagaaaa atacctgcac
     4741 ctcgggcttc tttt
//
LOCUS       DASSFD010000154         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_946828, whole genome shotgun sequence.
ACCESSION   DASSFD010000154 DASSFD010000000
VERSION     DASSFD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946828"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFH39_03775"
     CDS             complement(<1..395)
                     /locus_tag="VFH39_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924919.1"
                     /translation="MDKKHLHRVWRTFRRIHPAYFLLLTLVSGGICIYALRANNQHML
                     ALRQTLYHADQTGGDVTGALQTLQAYVTTHMNTELTTGNTSVYPPIQLVGTYDRLVQA
                     QSSQLQQQNSTIYTQAQDYCEKTYPSASL"
     gene            521..1816
                     /locus_tag="VFH39_03780"
     CDS             521..1816
                     /locus_tag="VFH39_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET6924920.1"
                     /translation="MHIYFSGIGGTAIGPLAMIARQAGFEVSGSDKQDSQYLHYLHKH
                     GIRNIHIGQSREQIASVHEAMPIDWFVYSSALPLENPDAPELLYCSEQGIKTSKRDEL
                     LNLILQEKGLKLVAVAGTHGKTTTTAMAIWLFKQLGIPVSYSVGAKIPFGDMGQYEPG
                     SEYFVYEADEFDRNFLAFEPHLSLITGVTWDHHEIFPTREDYQEAFKEFLAQSQHSFI
                     WQEDADYLSLEPGPLLSVLDSGARQIKNIRLLGQYNRLDAWLVAQAVHYITRKPLDEL
                     TTLLGEFPGLSRRMEQLAPDLYSDYAHTPEKIRGAMSVASEMAADKGQKVVVIYEPLT
                     NRRQHYMLDDYKDCFSGASKVYWLPSYLAREDPKQRIIAPEELISHLDNPDIAVPAEL
                     NEALKQTIQAHLNNGDLVIGMAGGGGGSLDEWLRHEFAS"
     gene            complement(2041..2382)
                     /locus_tag="VFH39_03785"
     CDS             complement(2041..2382)
                     /locus_tag="VFH39_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HET6924921.1"
                     /translation="MTFDDYQQKAITTDVYGGKGDVVSIAFINKVLGLVGESGEVADK
                     VKKLQRNNDGKMSNDERRELLKEVGDVLWYLSAITHYLGGSLNEVAEMNLEKLFDRKA
                     RGVIKSQGDNR"
     gene            complement(2379..2957)
                     /locus_tag="VFH39_03790"
     CDS             complement(2379..2957)
                     /locus_tag="VFH39_03790"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6924922.1"
                     /translation="MDTFEEATKQLSKSGAVGVIPTDTVYGLVARADDPEAVRRLYGL
                     KEREGKPGTLIACCIDHLVRLGLKGRYLKAVANYWPGPVSIIIPCGPELAYLHQGAGS
                     LAVRVPDDGTLVDFLEQTGPLLTSSANHPGRPVSTTIEEARAYFGGRVDFYVDGGNLS
                     GHQPSTIIRVVDDAIEVLREGAVKIENNGIKQ"
     gene            complement(3063..3353)
                     /locus_tag="VFH39_03795"
     CDS             complement(3063..3353)
                     /locus_tag="VFH39_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924923.1"
                     /translation="MNTDITPPAPDPAPTIEAPQPPVMDVQPPAPAPAPDVPADKQRS
                     KESPEPAKPAPKAVKPKAPKQPGSGVGFAIAATVVTVLGLAALATYAYLQTK"
     gene            complement(3388..4206)
                     /locus_tag="VFH39_03800"
     CDS             complement(3388..4206)
                     /locus_tag="VFH39_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924924.1"
                     /translation="MRLTPEAITAFQDQVLAYYHARGRHDLPWRRPEPDGSFDSYKIL
                     VSELMLQQTQVSRVVPKYTEFLRRFPSAGVLAAAPLGEVLRAWSGLGYNRRAKYLWQA
                     ARYLVDHHGGVVPDSNTELVKLPGVGKNTAGSILAYAYDRPVVFVETNIRTAYIHHFF
                     GDRTDITDAEILVAVEATLLRDDPRVWYWALMDYGAHLKQTIGNLNKLSKTYTRQSAF
                     AGSRRQLRGKILRTLGAGPAAAGELKIEDERLPAVLDELSTEGLIRLQNGRYTL"
BASE COUNT          900 a         1257 c         1191 g          934 t
ORIGIN      
        1 tgcagcgagg cggacgggta ggtcttttcg cagtagtcct gcgcctgggt atagatggta
       61 ctgttctgct gctgcagctg agagctctgc gcctgcacca gccggtcata ggtaccgacc
      121 agctggatag gcgggtacac cgacgtgttg ccggtggtca gttcggtgtt catgtgcgtg
      181 gtcacgtacg cctgcagcgt ctgcagggct ccggtgacat caccgccagt ctggtcggca
      241 tggtagagcg tctgcctgag ggcaagcatg tgctggttgt tggcgcggag cgcgtagata
      301 cagatgccgc cgcttaccag cgtcagcagc aagaagtacg ccggatggat ccggcggaac
      361 gtacgccaaa cgcgatgcag gtgcttttta tccataagca ccattttaca ggtaacaggt
      421 aacattccgc aggaattcgg caagcgcccg gtgtcagatg agaaagtcta ctgtccgcta
      481 tctgtttgtc tgttacatgc ttgtaagata acctagaagc atgcacatct actttagcgg
      541 cataggcggc acggccatag ggccgctggc tatgattgcc cggcaggccg gttttgaggt
      601 ttccggctcc gacaaacagg attcgcagta cctgcactat ctgcataaac acggcatacg
      661 gaacatccac atcgggcaat cgcgcgaaca gatcgccagc gtacacgagg ccatgccaat
      721 agattggttc gtgtacagct ccgccctgcc gctggagaac cccgatgcac cggaactgct
      781 gtactgcagc gagcagggca taaaaaccag caagcgcgac gagctgctga atctgatatt
      841 gcaggaaaag ggccttaagc tggtggccgt ggccggcacg cacggcaaaa ccaccactac
      901 cgctatggct atctggttat tcaagcagtt gggcataccc gtcagctatt cggtcggtgc
      961 caagatcccc ttcggcgaca tgggtcagta cgaaccgggg agcgagtatt tcgtgtacga
     1021 agccgacgag ttcgaccgca atttcttggc cttcgagccg cacctgagcc tcataaccgg
     1081 cgtcacctgg gaccaccatg agattttccc gacccgcgaa gattaccagg aagcgttcaa
     1141 ggaattcctc gctcagagcc agcacagctt catctggcag gaggacgccg actatctttc
     1201 cctggagcca ggcccgctgc taagcgtatt ggacagcggc gcccggcaga tcaaaaacat
     1261 ccgcctactc ggccagtaca atcgcctgga cgcctggctg gtggctcagg ccgtccatta
     1321 cattaccagg aagcctttgg atgaactgac gaccctgctc ggcgaatttc cgggcttaag
     1381 ccggcgcatg gaacagctgg cccccgacct ctacagcgat tacgcccata cccccgaaaa
     1441 gatccgcggc gccatgagtg tggccagcga aatggccgcc gacaaaggcc agaaggttgt
     1501 cgtcatctac gaacctttga ccaatcgccg tcagcactac atgttagacg attacaaaga
     1561 ctgcttcagc ggagccagta aagtctactg gctgcccagc tacctggccc gcgaagaccc
     1621 taaacagcgc atcatagcgc cggaagagtt gatatcgcac ctggacaacc ccgatatagc
     1681 cgtaccggcc gagctgaacg aagctctcaa gcagacgatt caggcccatc tgaacaacgg
     1741 cgatcttgtg ataggtatgg ccggtggtgg tggcggctcc ctcgacgaat ggctccgcca
     1801 tgagttcgct agctagttgc ctgccgcccc cgccggagac atcgggaaag ttcactgcgt
     1861 caaacaggcc ccaccggcac aaattcaggt cttcacctgc accacaccgg acattacaaa
     1921 cccatgatcg ctcacccgcc ggcaggggca ggcgcatcat cttctcctct tggagataat
     1981 tacgtattag ctactccgca tcgggcatca gtctttacgg tttgcgaaag gcagctttac
     2041 ttagcggttg tcgccttggc ttttgatgac accacgggct ttgcggtcga agagcttttc
     2101 gagattcatt tccgcgacct cattcagtga gccgccaaga tagtgcgtaa tggcgcttag
     2161 gtaccacagc acgtcgccta cttctttcag gagttcgcgg cgttcgtcat tgctcatttt
     2221 gccatcgttg ttgcgctgta gctttttgac cttgtcggct acctcgccgg attcccccac
     2281 cagcccaagc actttattta tgaaggctat ggagaccaca tcacccttgc cgccgtatac
     2341 gtcggtagtt atggctttct gttggtagtc gtcgaatgtc attgttttat tccgttgttt
     2401 tctatcttaa cagcgccttc gcgcaatact tctatggcgt cgtctacgac gcggatgatg
     2461 gttgatggtt gatggcccga gagattaccg ccgtccacgt aaaaatccac acggccgcca
     2521 aaatacgctc tggcctcttc aattgtagta gataccggcc ggccggggtg attggcgctg
     2581 ctggtcagca gcgggccggt ctgctccaaa aagtctacca gcgtaccgtc gtccggcacc
     2641 cggacggcca aactgccggc gccctgatgc aggtacgcca gttccgggcc gcagggaatg
     2701 atgatactga cgggaccggg ccaatagttg gcgacggcct tgaggtagcg gcctttgagc
     2761 cccagtctca ccaaatggtc aatgcagcag gcaataagag tgccgggctt gccttcacgc
     2821 tctttaagac cgtacaggcg cctgaccgct tccggatcgt ccgcacgcgc caccaggccg
     2881 tacaccgtat cggtgggtat gacccccacc gccccggatt ttgacagttg tttcgttgct
     2941 tcctcaaaag tatccatagc gcactcaatt aaccattagc gtgccgcatg caaccattaa
     3001 tctgcagcgg cggacctgct atatattcac acgccgcagt atcgtaacgg gtgacaggct
     3061 aattatttgg tctgcaggta ggcataagtt gccagcgccg ctaatcctag cacagtaaca
     3121 accgtagccg caatggcaaa tcctacaccg ctgccgggtt gcttgggagc cttgggcttc
     3181 accgccttag gggcaggctt ggccggttcc ggtgattctt tggaccgctg cttgtcggcc
     3241 gggacatcgg gcgcaggagc cggagcgggc ggctgcacgt ccatgaccgg aggctgcggc
     3301 gcctcaatgg tcggggcggg gtcgggagcc ggaggagtga tatctgtatt catgatgctt
     3361 acgtttacct tattgtatca tgcccgacta gagcgtatag cggccgtttt gcaatcggat
     3421 caggccttcg gttgacagct catccagaac cgccggcagc cgttcgtctt caatcttcag
     3481 ttccccggcc gctgccggcc cagcgcccag cgtccgcaaa atttttcccc gcaactgccg
     3541 ccgcgagccc gcaaaggctg attgccgcgt gtacgtttta cttaacttgt taaggttccc
     3601 tattgtttgt ttgagatgtg ctccgtaatc catgagtgcc caataccaaa cccgcggatc
     3661 atcgcgaagc aaggtcgctt ctaccgcaac cagaatctct gcatctgtaa tatcggtgcg
     3721 gtcaccgaag aaatgatgga tataggcagt ccggatattg gtttccacga atactacggg
     3781 ccggtcgtag gcataggcca ggatcgagcc ggcagtattc ttgcctactc caggaagctt
     3841 aacaagttca gtattgctgt ccggcaccac gccgccatgg tggtccacca ggtagcgggc
     3901 cgcttgccac aggtatttcg cccggcggtt gtaccccagc ccgctccagg cgcgcagcac
     3961 ttcccccagc ggcgccgctg ccagcacgcc ggcgctcgga aaacgccgta agaactcagt
     4021 atatttagga acgacccggc taacctgcgt ctgctgcagc atcagctcgg aaaccagtat
     4081 tttgtaggag tcgaagctgc cgtccggctc gggccgacgc cacggcagat catgccggcc
     4141 gcgggcgtga taatacgcca atacttggtc ttgaaatgcc gtaatcgctt ccggggtcaa
     4201 ccgcatcgta gtgtattata agggtcaaac catgattgat tatagctggc ggcttgccaa
     4261 acgctttgcc gtgctggtgc cg
//
LOCUS       DASSFD010000155         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_763502, whole genome shotgun sequence.
ACCESSION   DASSFD010000155 DASSFD010000000
VERSION     DASSFD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763502"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..582
                     /locus_tag="VFH39_03805"
     CDS             151..582
                     /locus_tag="VFH39_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6924925.1"
                     /translation="MNETPQPSAAAAPTDGPGQHPPRILLVEDDDALANVYTMRLQAE
                     HFDVRRVANGEDALATALSYKPNLILLDVMMPKVSGFDVLDILRNTPETANLKIIMLT
                     ALSQESDKERARSLGVDDYLVKSQVVIADVIERIKHHLNLE"
     gene            676..939
                     /locus_tag="VFH39_03810"
     CDS             676..939
                     /locus_tag="VFH39_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924926.1"
                     /translation="MPIRKLSDILRTGASAGISSQELKDIMQEDQREIDSKAVPAVIG
                     EGNDSFSDAQIVEAVGQAVQISQHIGSELLAGMGVEDPTEGEQ"
     gene            942..1559
                     /locus_tag="VFH39_03815"
     CDS             942..1559
                     /locus_tag="VFH39_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924927.1"
                     /translation="MAGFGAPNQYPQSSGEEVVTSNEFHLESAEAYRLYNFPNAVAQV
                     YDSARRQAGSETVDHHTTDSQICDIASNAVADSAMSEDAKALTRIMAAGATHDIRVAS
                     SLLQPVLESGRLNNTDRADFEAVVGNVHYLRGAFTEDSDKARAVVEAVSQDAFYAGTR
                     RPALRGALLAYLAGASAEFPEQSGAGYTMQSLVEQAGGAPRTSFS"
     gene            complement(1699..1971)
                     /locus_tag="VFH39_03820"
     CDS             complement(1699..1971)
                     /locus_tag="VFH39_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET6924928.1"
                     /translation="MSHVKAGGTSKNTRDSQGQRLGVKLFGGQTVKTGQVIVRQVGLT
                     KRAGKGTMLSSNYTIHAARDGVVKFTTRRVKRFTGKTVARTEVTVE"
     gene            complement(1979..>2172)
                     /locus_tag="VFH39_03825"
     CDS             complement(1979..>2172)
                     /locus_tag="VFH39_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924929.1"
                     /translation="TRLCLAVPAWQTSANQFKHLPLIDQIRDLGYNRVSFEHVRDDQL
                     LYYREDQIVARQLLVLTRK"
BASE COUNT          501 a          616 c          593 g          462 t
ORIGIN      
        1 gaaatcatcg aggccctcga ccaccccttc ctgctggcca gtcagttcca cccggagttc
       61 aagagccgac cgaaccgggc tcacccgatg ttcgacggat ttatcgccag ctgcttgtca
      121 aacaaggcca aataagttag cgtaaacagt atgaacgaaa cgcctcaacc ttccgccgct
      181 gccgcaccga ccgacggacc ggggcaacac ccgccacgca tcctgctcgt cgaggacgac
      241 gacgccctcg ccaatgtcta caccatgcgc ctccaggccg agcatttcga cgtccgccgt
      301 gtggcgaacg gggaagacgc cctggcgaca gccctcagct ataaaccgaa ccttattctg
      361 ctcgacgtca tgatgcccaa ggtcagcggt ttcgacgtgc tcgacatctt gcgtaacacg
      421 cccgagacgg ccaacctcaa gatcatcatg ctgaccgccc tcagccagga aagcgacaaa
      481 gagcgtgccc ggtcgctggg cgttgacgat tacctggtaa agtcgcaggt cgtcatagcc
      541 gatgtcatcg agcgtatcaa gcaccacctc aacctggaat agaccactta gcaaagcaac
      601 gtttcatagc tgtcacaccg acagctattt tcatatctaa ttgacttttt acaaagctta
      661 tgctaatgtg tacctatgcc aataagaaaa ttatcagata tactccgaac tggcgcatct
      721 gccggtattt cgagccagga gctcaaagac atcatgcagg aagaccagcg cgaaattgat
      781 tccaaagccg tacccgctgt tatcggtgaa ggcaatgact ccttcagcga cgctcagatt
      841 gtcgaggctg taggtcaggc agtccaaatc tcacagcata tcggcagcga actgctcgct
      901 ggcatgggcg ttgaagaccc aaccgaaggg gagcagtaat catggcaggt tttggcgctc
      961 ccaatcagta tcctcagtcc tcaggcgaag aagtagtgac cagtaacgaa ttccatctgg
     1021 aaagcgctga agcgtaccgt ctgtataact ttccaaacgc cgtggcccag gtctacgatt
     1081 ctgcccgacg acaggcgggc agcgaaacag tcgatcacca tacgactgac tcccagatat
     1141 gtgacatagc aagtaatgcg gtggctgact ccgctatgag cgaagatgcc aaggcattaa
     1201 cccgcatcat ggctgccggc gcaacgcacg acatacgagt ggcgagctcg ttgctgcagc
     1261 cggtacttga atcgggacgt ttaaataaca ctgaccgtgc agatttcgag gcggtcgtcg
     1321 ggaatgtgca ttatcttcga ggcgccttta cggaagacag tgataaagcg cgggcagtcg
     1381 ttgaagctgt cagccaagat gctttttatg ctggcacacg acgtccggcc ttacggggcg
     1441 cgctcttggc gtacctcgcg ggtgcatcag ccgaatttcc ggagcaatcg ggtgccggct
     1501 ataccatgca atcgttggtt gaacaggcag gtggtgcccc cagaacatct ttcagctgac
     1561 gcgccactgt cctgcgacca aaaaagcccg gaagggcttt ttcatatttg ctgcgattac
     1621 tactcaaccg tgacttcggt gcgcagaaat gcccgctggg catttgctgt cgatgctagg
     1681 gtaccgtcgt tgacgacgtt actcaaccgt gacttcagtg cgggcaacgg tcttgccggt
     1741 gaagcgcttg acgcggcggg tggtgaactt cacgacaccg tcacgggcag cgtggatggt
     1801 gtagttgcta ctcagcatgg tgcctttgcc ggcgcgcttg gtaaggccta cctgacggac
     1861 gatgacctga ccggtcttga cggtctggcc gccgaacagc tttacgccga gccgctggcc
     1921 ctggctgtcg cgcgtgtttt tactggtacc gcctgctttg acgtgtgaca tatgattatt
     1981 acttcctcgt taatactagg agctgacggg cgacgatctg atcttcacgg tagtaaagga
     2041 gctgatcatc ccggacgtgc tcaaaactca ctcgattata gcccaaatcg cgtatctggt
     2101 caataagggg taagtgcttg aactggttcg cgctggtttg ccaggcgggg acggccaggc
     2161 agaggcgcgt gc
//
LOCUS       DASSFD010000156         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_696185, whole genome shotgun sequence.
ACCESSION   DASSFD010000156 DASSFD010000000
VERSION     DASSFD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696185"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..198
                     /locus_tag="VFH39_03830"
     CDS             43..198
                     /locus_tag="VFH39_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924930.1"
                     /translation="MKIIEVKNDPLTVRLPEWIDTNQVYEAFWSLTAEQWLIVPTGKV
                     REDVIPF"
     gene            211..894
                     /locus_tag="VFH39_03835"
     CDS             211..894
                     /locus_tag="VFH39_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HET6924931.1"
                     /translation="MSESKQLNIYQRLLEVQHEVKAPKQAGGRFGKARSAEQVLAEAK
                     PVCYKHGLYLFTQDAVEYVEGRHYIVGTATVVNVDNPEETHSAKAYAWEGEQDRSLDT
                     AQVSGKTSSYAKKYALQHLLGIDDTKDADFDQEATSKQTAQPSVAAPKPAAPKPEGVT
                     KAQLSILFPMMRGKGYEDKEQAKRILHALAGVESLTQLTKEDAGRLINEVSAASREDL
                     DLLDSEVAA"
     gene            891..1049
                     /locus_tag="VFH39_03840"
     CDS             891..1049
                     /locus_tag="VFH39_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924932.1"
                     /translation="MSKAVFLVTPDNHPNVVPYQAPDVKNWLNHELARIADYSKRQVV
                     VIVEVAEL"
     gene            1046..1207
                     /locus_tag="VFH39_03845"
     CDS             1046..1207
                     /locus_tag="VFH39_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924933.1"
                     /translation="MSFDLQAAFDQIDKRLKHNRDCPNGECVDMDDDLTVSGSDWELT
                     EYGASADEY"
     gene            1197..1352
                     /locus_tag="VFH39_03850"
     CDS             1197..1352
                     /locus_tag="VFH39_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924934.1"
                     /translation="MNTKVLMLSRLHKQRRATEISLKEYPLAYEPLPSEAKQRKMWRL
                     IARRKKR"
     gene            1444..1638
                     /locus_tag="VFH39_03855"
     CDS             1444..1638
                     /locus_tag="VFH39_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924935.1"
                     /translation="MTILAAHDLLVLYFTITGLAVGVPLGLYAITLLLYQAGRSITGL
                     VQTKQDLQPVWNPNHNTKRK"
     gene            1605..1847
                     /locus_tag="VFH39_03860"
     CDS             1605..1847
                     /locus_tag="VFH39_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924936.1"
                     /translation="MEPQPQHEAQIIPFPERRMEPVGTERINGVKLPLGRLCLDDLLG
                     IQYHCVVRLDNARHELSVVTDAIDRRFGPEVPDGAA"
     gene            1844..2059
                     /locus_tag="VFH39_03865"
     CDS             1844..2059
                     /locus_tag="VFH39_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924937.1"
                     /translation="MSGLDHGPLAGCTYRQCGVKGDEANCLFTKDLAAEPQPNWQASC
                     NHEYRYIGMGSFYCVYCLDIQKKPIRP"
     gene            complement(2056..>2348)
                     /locus_tag="VFH39_03870"
     CDS             complement(2056..>2348)
                     /locus_tag="VFH39_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924938.1"
                     /translation="LRTQKAGAAVAGPLGPKHRLRSNSQAYAIRSGAFRDIVVYTLKR
                     MGRDFTHCEWCGKHLDDGKWQLHHTKYEGATVDDLMIVCQQCNLIPANRFLD"
BASE COUNT          580 a          695 c          684 g          389 t
ORIGIN      
        1 cctaatggct ctcggctacg ccctgggctg gggaatctag ccatgaagat aatcgaagtg
       61 aagaacgacc cgctgacggt acgcctgccg gagtggatag acaccaacca agtatatgag
      121 gcgttctgga gcctgactgc cgagcagtgg cttatcgtgc cgaccggcaa ggtgcgcgag
      181 gacgtaattc ccttttagga aggaagtggt atgagtgaga gtaaacaact aaacatttac
      241 cagcggctac tggaagtcca gcacgaggta aaagcgccga agcaggccgg cggccgtttc
      301 ggtaaggccc gcagtgccga acaggtactc gcagaggcca agccggtctg ctacaagcac
      361 ggcctctacc tattcaccca ggatgccgtc gagtacgtcg agggccgcca ctacatcgtc
      421 ggcactgcca ccgtcgtcaa cgtcgacaat cccgaagaaa cgcactcggc caaggcatac
      481 gcctgggaag gcgagcaaga tcggtcgctc gatacggcac aggtaagcgg taagaccagc
      541 agttacgcca agaagtacgc cttacagcac ttgcttggca tcgacgacac caaggacgct
      601 gacttcgacc aagaagcaac gtctaagcag accgcgcagc cgagtgttgc cgcaccaaaa
      661 ccggccgccc ccaagcccga aggcgtcacc aaggcccagc tgagcatcct attcccgatg
      721 atgcggggta aaggctacga ggacaaagag caagccaagc gcatattgca cgccttggcc
      781 ggcgtcgaaa gcctgacgca gctcaccaaa gaagacgcag gccgcctcat aaacgaggta
      841 agcgcggcga gcagggaaga cctcgacctg ctggatagcg aggtcgcggc atgagcaagg
      901 cagtatttct agtgactccc gataatcacc cgaacgtcgt tccatatcaa gcgccggacg
      961 tgaagaactg gctgaaccat gaactagcga ggattgccga ctacagcaag cggcaggtgg
     1021 ttgtcatcgt tgaggtggca gagctatgag cttcgacctc caagccgcct tcgaccagat
     1081 agataagcgc ctcaagcata accgggactg cccgaacggc gagtgcgtcg atatggacga
     1141 cgacctgacc gtaagcggga gcgactggga gctgacggaa tacggagcat cagccgatga
     1201 atactaaagt cctgatgctg tcccggctgc acaagcaacg ccgcgccacc gagataagcc
     1261 tgaaagaata cccgctggca tatgagccgc tgcccagcga ggccaagcaa cgtaaaatgt
     1321 ggcggctcat cgccaggagg aagaaacgat gattggctgc tactgccccc agtgcggggc
     1381 gcactacacg actaacacgg cgctcaagct gcaccgcgag ataagccacg acgaggtgaa
     1441 ggtatgacaa tcctcgccgc ccacgaccta ctcgtgctgt acttcacaat caccggcctg
     1501 gctgtcgggg tgccgctcgg gctatacgcc ataacgctat tgctttacca ggccggccgt
     1561 tctataacgg ggcttgtaca aactaaacag gacttacaac cagtatggaa ccccaaccac
     1621 aacacgaagc gcaaataatc ccgttcccgg aacggcgcat ggagccggtc ggcacggaac
     1681 gcatcaatgg cgtgaagctg cccctgggcc ggctgtgcct cgacgacctg ctcggcatcc
     1741 agtaccactg cgtcgtgcgc ctggataacg cccggcatga gctgtcggtg gtgacggacg
     1801 ccatcgaccg gcgcttcggg ccggaagtac cggacggggc ggcatgagcg ggttagatca
     1861 cgggccgctg gccggctgca cctaccgcca gtgcggcgtg aagggcgacg aggcgaactg
     1921 cctgtttacc aaagacctag cagccgagcc gcaaccaaat tggcaggcga gctgcaacca
     1981 cgagtaccgc tacatcggca tgggtagctt ctactgcgtc tactgcctcg atattcagaa
     2041 aaaacctata agaccctagt ctaggaagcg atttgccgga atgaggttgc actgctggca
     2101 aacaatcatg aggtcgtcaa ccgttgcacc ctcgtacttg gtgtgatgca gctgccactt
     2161 tccatcgtcg agatgcttgc cgcaccactc acagtgcgta aagtcccgac ccattcgctt
     2221 gagagtatag acgacaatat cccggaaggc gccgctccga atagcataag cctggctatt
     2281 actacgtaat cggtgctttg ggccgagggg gccagcgaca gccgcacccg ccttttgcgt
     2341 gcggagca
//
LOCUS       DASSFD010000157         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_562135, whole genome shotgun sequence.
ACCESSION   DASSFD010000157 DASSFD010000000
VERSION     DASSFD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562135"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFH39_03875"
     CDS             <1..432
                     /locus_tag="VFH39_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6924939.1"
                     /translation="PDLTPETRRDNTRNMQLATLDVGGSKLHVANHHAHWVMSPVGDE
                     VSVEKMQLVAQKVRGLEGPVIVAGDLNVTAASPAMRVFDGLLTDLTATHDIPDTLSEL
                     GKVRNVPCDHILVNDQVKVNSFAVRDDLVSDHKTLVMEFEL"
     gene            complement(487..819)
                     /locus_tag="VFH39_03880"
     CDS             complement(487..819)
                     /locus_tag="VFH39_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HET6924940.1"
                     /translation="MTFDEYQQQAIKTNLTNDDRFKELMQQVLGLADESGEVLAKFKK
                     WIRDDGADFDKLDLANIKKEMGDVLWYIAVIAHDLGISLDDLAQTNIDKLASRHQRGT
                     LRGSGDDR"
     gene            complement(816..1379)
                     /locus_tag="VFH39_03885"
     CDS             complement(816..1379)
                     /locus_tag="VFH39_03885"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6924941.1"
                     /translation="MFDQILPKLAVPGCVGVLPTDTVYGLVARAADESAVKRLYDLKF
                     REGKPGTIIAASTDQLVGLGLKARYLKAVEQFWPGAVSVIIPCGPELTYLHEGKFSLA
                     VRIPDNQELLNLLSKTGPLLTSSANHPGEPTATTLEQANAYFNGNVDFYIDGGDLSNH
                     LPSTIIRIVDDAIEIIRQGAVKVQENE"
     gene            complement(1428..1835)
                     /locus_tag="VFH39_03890"
     CDS             complement(1428..1835)
                     /locus_tag="VFH39_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924942.1"
                     /translation="MRENGGESNHSAQPAQPAGRHPVMDVVRRPVDPPATRAEIAPPP
                     DVMKQIEAGGDVPPDMPHQPAMDVVKEPPREDDTEPTPHPEPVHKPESHAPAPKPVKP
                     AQASTGVTGAIVATVVIVLVLSGLAVFAYLQSK"
     gene            complement(1879..2700)
                     /locus_tag="VFH39_03895"
     CDS             complement(1879..2700)
                     /locus_tag="VFH39_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924943.1"
                     /translation="MKNIDTSGFRKMLAEQYRLHGRPHLPWRLPEPDGSFDPYKILVS
                     EVMLQQTQVTRAIPKYHQFLESFPNVEALAAASLGDVLVVWSGLGYNRRAKFLWQAAQ
                     TLVREYGGVFPRTVDELIRLPGIGRNTAGAVLAYAFNQPVVFIETNIRSVYIHHFFAD
                     ADVVADKELQPLIGATLDTGAPREFYWAVMDYGSYLKRSAGNHTRRSRHYVRQSKFKG
                     SNRQIRGQVIRLLAQRHMSAAELAELISDDRLEAVLATLMSEGLIVQSKGYYRLA"
     gene            2726..3565
                     /locus_tag="VFH39_03900"
     CDS             2726..3565
                     /locus_tag="VFH39_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HET6924944.1"
                     /translation="MIDYVLRLAKRFAVLVPGIVIAYFSVRDIFPIVDKRLPVAVAVL
                     VTYILGAYVLIPAATRVVRIFFPAHHLPLYCVTPDGFASDPLNIAVVGSKRRLVRAMR
                     AAGWHVADNHSLPNVFREVLSALFNRPYLTAPMSSLYLFGRKQDIGFEIPIPGERGHR
                     HHVRFWATTYENNGQLTAKKIGWHQRKERLSGSDLLWVGAASRDVGFAVIKHNVQITH
                     MIHPDTGSERDLIEKGLDEAGWLASSEDVRLGRPYKLTNRALGGHLRTDGVLRVIRLV
                     NKT"
     gene            complement(3578..>5116)
                     /locus_tag="VFH39_03905"
     CDS             complement(3578..>5116)
                     /locus_tag="VFH39_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924945.1"
                     /translation="KKARWATVIVVVLLVAAAAAWPTSRAWALNLVGVRASADVTVLD
                     GITKLPLRDVDVSIGGSQGRTNSQGEVSLGLVKLGTQQLRVRKRAFAVSYTSEHIGLG
                     VNHLGTVNLRPAGDRYQLRAIDYVSGKPVAAAMAVAGDAEAIADKQGIITLTISGVNA
                     GDNVSFKAYVSADGYRTQPVNLTNAQSGVTTVQLVTTRPDVYFSNQSGKYDLYKSDID
                     GQNKKLLVAGTGSETSSLSLLVHPSGAEAAFVDTRDGTHDSDGYLEQSLSLVNVSTGA
                     LTTIDHSDRIQLVDWVGDRIVYIKTKAGASAANPSRYQMLSYDYRINQRLTLDHANAF
                     NDVVSTDGSIYYATSNQYDGGVSQFVSIHADATNRQVLLNTEVWNILRKDYDDFYLSS
                     GNANYAYKLGDAKPTATNETDNNPSRLYVDSPDGKHSLWVDQRDGKGVLLSYDLATQK
                     DTVIAELPGITYPVRWLSNDTIIYRVKTYNETADYVIGLSSNTSKKITDVTDASGKSL
                     WYFY"
BASE COUNT         1015 a         1473 c         1481 g         1147 t
ORIGIN      
        1 cccgacctga caccggagac acggcgtgac aatacccgca acatgcagct ggcaacgctg
       61 gatgtaggcg gctcaaagct ccatgtggcg aatcatcacg cgcactgggt aatgtcgccg
      121 gtgggcgatg aagtaagcgt cgaaaaaatg cagctggtcg cccaaaaagt ccgtgggctg
      181 gaaggcccag tcatagtagc cggcgacctg aacgtcaccg ccgcctcgcc cgctatgcgc
      241 gtgttcgacg gcctactcac cgacctgacg gccacgcatg atattccaga caccttgagt
      301 gagctaggaa aggtacgtaa tgtcccctgc gatcacatcc tggtcaatga ccaggttaag
      361 gtcaattcgt tcgctgtccg tgacgacctg gtctccgatc acaaaaccct ggtgatggag
      421 ttcgaactgt agcaagaatt agcgtgccgt ctgccggccg acttagcgta ccgctattgt
      481 agcattctag cggtcgtcgc cgctgccgcg gagggtgccg cgctggtggc ggctagcgag
      541 cttgtcgata ttggtttgag cgaggtcatc gagtgaaatg ccgaggtcgt gggcgatgac
      601 ggctatgtac cacagcacgt cacccatctc tttcttgatg ttagcgaggt cgagcttgtc
      661 gaagtcggca ccgtcatcac ggatccactt tttgaacttg gccaatacct cgccggattc
      721 atcggccaga cccagcacct gctgcatcag ttccttgaac cggtcatcgt tggtcagatt
      781 ggtcttgatg gcctgctgtt gatactcgtc gaatgtcatt cgttttcctg cactttcacc
      841 gcgccttggc ggattatttc gattgcgtca tcaacgatgc ggataatggt cgatggtaag
      901 tggtttgaga ggtcaccgcc atcgatataa aagtccacgt tgccgttaaa atacgcgttc
      961 gcctgctcaa gtgtggtggc tgtcggctcg cccggatggt tggcgctgct ggtcaggagc
     1021 ggaccggttt tacttagcaa gttaagtaat tcctgattat cgggaatgcg gaccgccaga
     1081 ctgaacttgc cttcgtgcag gtacgtcagt tccgggccgc agggaataat gacactgacc
     1141 gcaccgggcc agaactgctc taccgctttc aggtagcgcg ccttcaggcc tagcccgacc
     1201 agttggtccg tgctggcggc gatgatggtt cccggcttgc cttcgcggaa tttcagatca
     1261 tacagccgct tgacggccga ttcatcggca gcgcgggcaa ccaaaccgta gaccgtatcg
     1321 gtcggcagca cgccgacgca acccggcaca gccagcttcg gtagtatttg gtcaaacatc
     1381 agccagatat tgtaccccaa aaataagccg tgtgataggg gtgcgggcta tttggattgc
     1441 aggtaggcga acaccgccaa gccggacagc accaggacga tgacgaccgt ggcgacgatc
     1501 gcaccggtaa ctccagtgga tgcctgggca ggcttgacgg gttttggcgc cggcgcgtgc
     1561 gactcaggtt tatgaaccgg ctcgggatgg ggtgttggct cggtatcatc ttcgcgtggc
     1621 ggttccttga ctacatccat ggcgggttga tgcggcatgt cgggcggcac atccccacct
     1681 gcctcgatct gcttcatcac atccggcggc ggagcgattt cggcgcgcgt agccggcgga
     1741 tcgaccgggc gtcggacgac atccatgacc ggatggcggc cggccggttg ggcaggctgc
     1801 gctgaatgat tcgactctcc accgttttca cgcattttct atggtcttct tgagacttat
     1861 tgtatcacgc ttttgctttc aggccaggcg gtaatagccc ttgctttgca caatcaaccc
     1921 ttcggacatt aaggtggcaa gtaccgcctc caggcggtcg tcagagataa gttccgccag
     1981 ctctgccgca gacatatgcc gctgcgctag caagcgaatg acctgcccgc ggatttgcct
     2041 attagaacct ttgaacttcg actgccgcac gtagtggcgg ctgcgccgcg tatggttacc
     2101 ggcgctccgc ttgaggtacg aaccgtaatc cataacggcc caataaaact ctcgcggagc
     2161 accagtatcg agcgtcgctc cgatgagcgg ctgcagctct ttgtcggcca caacgtccgc
     2221 gtcggcgaag aagtggtgga tatacacgct gcggatattc gtctcgatga acaccaccgg
     2281 ctgattgaag gcatacgcta gaacggcgcc ggccgtattg cgaccgatgc ccggcaaccg
     2341 gatcagctcg tcgaccgtac gtggaaacac accgccgtac tcacggacga gcgtctgtgc
     2401 tgcctgccac aggaatttgg cgcgccggtt atagccaagg ccgctccaca cgaccagcac
     2461 gtcacccaag ctggccgcgg ccagagcttc tacattcggg aacgactcca ggaactggtg
     2521 atactttgga attgcccgcg ttacctgcgt ctgctgcagc atcacttcgc tgaccaaaat
     2581 cttatacggg tcgaagctgc cgtccggctc gggcaggcgc cagggcaaat gcgggcggcc
     2641 atgcagccga tactgctcag ccagcatttt cctgaagcca gatgtgtcga tatttttcat
     2701 gacagctcct gtattataaa tgcagatgat cgattacgtg ttgcggctgg ccaagcgctt
     2761 tgccgtgttg gttccgggta ttgttatcgc ttacttttcg gtgcgcgata tatttcccat
     2821 cgttgacaag cggctgccgg tagcggttgc cgtgctggtg acctacatac tgggagcgta
     2881 tgtactgata ccggccgcaa cccgggtggt ccgcatattc ttcccggcgc atcacctgcc
     2941 gttgtactgc gtgacccccg acggcttcgc ctccgacccg ctgaacatcg ctgttgtcgg
     3001 cagcaaacgt cggttggtac gcgccatgcg ggccgccggc tggcacgtcg ccgacaatca
     3061 ctcgctcccg aatgttttcc gggaagttct gagcgccctg ttcaaccggc cgtacctgac
     3121 cgccccgatg agcagtctgt acctattcgg tcgcaagcag gatatcggtt tcgagattcc
     3181 catcccgggc gagcgtggcc atcggcacca tgtacgtttc tgggccacca cctacgaaaa
     3241 caatggtcag ctcaccgcca aaaaaatcgg ctggcatcag cgcaaagagc gtctgagtgg
     3301 cagtgacctg ctgtgggtcg gcgccgcttc gcgcgatgtc ggtttcgccg taatcaagca
     3361 caacgtgcag attacgcaca tgattcaccc cgataccggc agcgagcgtg acttgatcga
     3421 aaaaggcctg gacgaagccg gttggctagc cagcagcgaa gatgtccggt taggccgccc
     3481 ctataagctt actaaccgag ccctgggcgg tcacctgcgt accgacggag tcctgcgcgt
     3541 catccggctg gtcaataaaa cttagcttgt aaagtattta gtagaaatac cacagggact
     3601 tgccgctggc atcagtgaca tccgtaatct ttttggaggt gttgctgctg agaccgatga
     3661 cataatcggc cgtctcgtta taggttttga cgcggtaaat gatagtgtcg ttgctgagcc
     3721 agcggaccgg atacgttatg cccggtagct cggcgatgac agtgtccttc tgggtggcga
     3781 ggtcgtatga cagcagcaca cctttgccgt cgcgctgatc aacccacaag ctatgcttac
     3841 cgtctgggct atctacgtac agccggctcg gattattatc ggtttcgttg gtagccgtgg
     3901 gcttggcatc gcccaattta tacgcgtagt tggcgttgcc gctgctgagg taaaaatcgt
     3961 catagtcctt gcggaggatg ttccacacct cggtgttcag cagtacctga cggttagtgg
     4021 catcggcatg tatgctgacg aattgcgaga cgcctccgtc gtattggttc gaggtggcgt
     4081 aatatatact gccgtccgta ctgacgacgt cgttgaaggc gttggcatgg tcgagcgtca
     4141 gccgctgatt gatacgatag tcgtagctca gcatctggta gcgcgacggg ttggccgcac
     4201 tggcgccggc cttggtcttg atgtatacga tacggtcacc gacccaatcg accagctgaa
     4261 tgcgatctga gtggtcaatg gtggtcagag ctccggtaga cacattgacg agcgacagcg
     4321 attgctccag gtagccgtcg ctatcgtgcg taccgtcccg ggtgtcgacg aacgccgcct
     4381 cggcaccgct ggggtggacg agcaacgata atgagctggt ttcgctgccg gttccggcta
     4441 ccagcaactt cttgttctgg ccatcgatgt ctgatttgta cagatcgtat ttgccgctct
     4501 gattgctgaa gtacacatcc ggacgggtag taacgagctg aaccgtggtg actccggatt
     4561 gcgcattagt taagtttacc ggctgcgtcc ggtaaccatc ggcagatacg tacgccttga
     4621 agctgacatt atcgcctgcg tttaccccag atatggtgag cgtgatgatt ccctgcttgt
     4681 cggcgatagc ctcggcatcg ccggccacag ccatagccgc agcgaccggc ttaccactga
     4741 cgtaatcgat agcccgcagc tggtaacggt ccccggccgg ccttagattg accgtgccga
     4801 gatggttcac gcccagcccg atgtgctcgc tggtatagct caccgcgaac gcgcgtttgc
     4861 gcacccttaa ttgctgagta cccagcttga caaggcctaa ggatacctca ccctggctgt
     4921 tagtcctgcc ttggctgccg ccgatgctga cgtcgacgtc cctcagcggt agcttcgtaa
     4981 tgccatccag gacggtcaca tctgcagaag cgcgcacgcc taccaggttc aacgcccagg
     5041 cacggctggt cggccaggcg gcagccgccg ccactaacaa cacgaccacg ataacggtag
     5101 cccaacgagc tttttt
//
LOCUS       DASSFD010000158         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_868873, whole genome shotgun sequence.
ACCESSION   DASSFD010000158 DASSFD010000000
VERSION     DASSFD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868873"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /gene="groL"
                     /locus_tag="VFH39_03910"
     CDS             complement(<1..1580)
                     /gene="groL"
                     /locus_tag="VFH39_03910"
                     /inference="COORDINATES: protein motif:HMM:NF009488.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6924946.1"
                     /translation="MAKKVFYDDDARRRVLAGAEVLYNAVKTTMGPKGRNVVISKGYG
                     SPTVTHDGVTVAKGVELADVDDETLGYKVGAELIKQAASKMNDVAGDGTTTVTVLTYH
                     ILNEANKLIAAGHNPMQLRKGLELAAQDVIGRLNTLSEDIQGKRTRVAEVATISAGDN
                     EIGNLIADVIDKVGKDGVVTVEEGQGLSLESEVVEGFTFDRGFVSPYMVTDTARMEAV
                     YDKPAVVVTDKKIGSIQEFLPLLEKLAQAGKKDLVLIAEDVEGEALGTLVLNRLKGVF
                     NTVAIKAPAFGDRRKDILNDIAILTGADVITEDRGMTFENVDLDVVGSARKVIVTKDE
                     TTIIEGSGTAAEVQARIKQIDAQVAAATSEYDKENLEKRRAALQGKVAVIKVGGATET
                     EIEEKKFRVDDAVAAVKAALDEGIVPGGGVTLINLAGGIKTDGADSAAAGAQLLKNAL
                     EQPFRILLTNAGLNADEWLPQVKNGKAGFGVDVNQPSKLVDLKTAGVIDPTRVTKEAL
                     QNAVSIAGTAMTMGALVV"
     gene            complement(1590..1856)
                     /locus_tag="VFH39_03915"
     CDS             complement(1590..1856)
                     /locus_tag="VFH39_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6924947.1"
                     /translation="MSAPLQPLGDYVVATAEEAETKTASGLYLPENAAEKPKTAKVVA
                     VGKDAKQVKAGDRIVYKSYSTTEVKVGGTEYILVKEEDILATVK"
     gene            complement(2130..2447)
                     /locus_tag="VFH39_03920"
     CDS             complement(2130..2447)
                     /locus_tag="VFH39_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924948.1"
                     /translation="MSKNRVELPTQRTSEPAAVTAKGFEHLQLSAMTDATSATRHAAV
                     LADTVLADTPPARIDLKRVDNGVVQHPPREIKHKVRGAAVTRRERILRAIDLRDIALD
                     RAG"
     gene            complement(2734..3306)
                     /locus_tag="VFH39_03925"
     CDS             complement(2734..3306)
                     /locus_tag="VFH39_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924949.1"
                     /translation="MSPEVPSAANFYPHTPEKELVELLLSKKIAASSIDEDIYKRRAA
                     EGREGWGLEHRPYVIPKEQNESLPCDCDQMRFEPRKDVSFGTSEIFAWIPRSGESNLV
                     FFNDVFIKVCYADGHPEELYLLNGEGLVTYRQATDLQDNMDDVARGGYMLDGDRFSVV
                     NTGPAGRLTAEELRIVLEPAITLTQDPIAG"
     gene            3427..>3895
                     /locus_tag="VFH39_03930"
     CDS             3427..>3895
                     /locus_tag="VFH39_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924950.1"
                     /translation="MFIELSVGQPVELDIAHTMLYGGVFSSGGRMYASLKFNFVLDEL
                     EQSHLVALSTQLLGKQTGLPVFQPGPRVTQVLLQLPAGSRHGRTDFLCREVAKRYGEW
                     LIQHLSDKAIRQSKASKKHQPAEQLAVVAFLPLPGAALATNPAPASPSRTRRAE"
BASE COUNT          742 a         1179 c         1049 g          925 t
ORIGIN      
        1 tcaaccacca gcgcgcccat ggtcatggcc gtaccggcga tgctgacagc gttctgcagc
       61 gcctctttag tcacacgggt agggtcgatc acgccggcag tcttcagatc caccagtttg
      121 ctcggctggt tcacgtctac gccgaaaccg gccttgccgt ttttgacctg cggcagccac
      181 tcgtcggcgt tcaggccggc gttggtgagc aggatgcgga aaggctgctc cagagcgttc
      241 ttgagcagct gcgcgccggc ggctgcagag tcggccccat cggttttgat gccgcccgcc
      301 agatttatga gcgtcacgcc gccgcccggc acgatacctt cgtccagggc agcctttacg
      361 gccgcaacag cgtcgtcgac acgaaacttc ttctcttcta tctcggtttc ggtagcaccg
      421 ccgaccttga tgacggctac tttgccctgc agagcggcgc gccgcttttc caggttctcc
      481 ttgtcgtatt cgctggtcgc agccgctacc tgggcatcaa tctgcttgat gcgcgcctgc
      541 acctcggcgg ccgtgccact accctctatg atggtcgttt cgtccttggt cacgataact
      601 ttacgggcgc tgccgacgac atccaggtca acattctcga acgtcatgcc gcgatcttca
      661 gtgattacgt cggcgccggt cagaatggcg atgtcgttta agatgtcttt gcggcggtcg
      721 ccgaaagccg gcgccttgat agcaacggtg ttgaagacgc ccttcaggcg gttcagcacc
      781 agtgtgccca gcgcttcacc ctccacgtct tcggctatca gcaccaggtc cttcttaccg
      841 gcttgtgcca gcttttcaag cagcggcaga aactcctgga tgctgccgat cttcttgtcg
      901 gtaaccacca cggcaggctt gtcgtacacc gcttccatgc gcgccgtgtc ggtcaccatg
      961 taggggctta cgaagccgcg gtcgaaggta aagccttcca ccacttcact ttccagactc
     1021 agcccctgcc cttcttccac ggtcaccacg ccgtccttgc cgaccttgtc gataacgtca
     1081 gcgataaggt tacctatttc gttgtcgccg gcgctgatgg tcgccacctc tgccacgcgg
     1141 gtccttttgc cttggatgtc ctcacttaac gtattgagcc tgccgattac atcttgggca
     1201 gccaactcca aacccttacg cagctgcata gggttatgtc cggcagcaat gagtttgttc
     1261 gcctcgttaa ggatatggta ggtcagcacg gtcacggtag tcgtaccgtc gccggccaca
     1321 tcgttcattt tggaagcggc ctgcttgata agttcagcac ctactttgta gcccagcgtt
     1381 tcgtcatcta cgtcggccaa ctccacgccc ttagccaccg ttacgccgtc gtgcgttacg
     1441 gtcggactgc cgtagccttt gctgatgacc acgttgcggc ccttggggcc catagtggtc
     1501 tttacggcgt tatataagac ttccgcgcca gcaagaaccc ggcggcgggc gtcgtcatca
     1561 taaaaaactt tctttgccat tagttcctcc tacttaacgg tcgctaaaat atcttcttct
     1621 ttaaccagta tgtattcggt accacccact tttacctcgg tagtactgta acttttataa
     1681 acgatcctgt cccccgcctt aacctgcttg gcgtctttgc ctacggcgac caccttggcg
     1741 gtcttgggct tctcggctgc gttctccggc aggtacagcc cgctggcagt cttggtctca
     1801 gcctcttcgg ccgtcgcgac gacgtagtcg cccagtggtt gcaatggtgc actcataatc
     1861 tacctctcct cctgtttagt cgttttgcat tagcactgta agcctgagag tgctaattat
     1921 cagctttcat aatactcaaa ttgacgatta gccgtcaata cccgcccaac taaaaacagg
     1981 cctgacggcc tgctgcagat atccatggaa gttgagattg tgagtgaagc gaggatggag
     2041 tccgggaggc ggcggtgttg tcacacctta agcgcttaaa gcgcatacct cccggacccc
     2101 tggcgatact gccttacgtc agaagattcc taccccgctc ggtcaagcgc gatgtcgcgc
     2161 agatcaattg cccggagaat gcgctcacga cgggtgacgg cggctccgcg cactttgtgc
     2221 ttgatctcgc gcggcgggtg ctggacaaca ccgttgtcga cccgcttaag gtcaattctg
     2281 gccggagggg tgtcagccaa gacggtgtca gccaagacgg ctgcatggcg ggtcgcgctt
     2341 gtggcgtccg tcattgcgct cagctggagg tgctcgaacc ccttggccgt aacggccgca
     2401 ggctcgctgg tgcgctgcgt gggcagctcg acgcggttct tgctcacggt gcctccccta
     2461 ggcaatcgtg atggccaatc gggcggttgc cagactggtt ggtacaaggt cttcgcttca
     2521 cttggaagca atagcggccc gtacgcgact ggcagccgtt gcttccaagc tctcacaatc
     2581 tcaaggtgca tccccgagcg gttaagctca aggggaatgg ctacataatc tgcagaggaa
     2641 gtatcagaat cccgacacca ggtctgtaaa ttatttcaca attatacgcc tcgtgctcat
     2701 atttgcaaga ccatatttat acaaaattgt aaactaaccg gctattggat cctgggtgag
     2761 tgtgattgcg ggctccagca ctatccgtaa ctcttcggcg gtcagccggc cggccggccc
     2821 ggtattgaca acactgaaac gatcaccgtc cagcatatat ccgccgcggg ctacgtcgtc
     2881 catgttgtcc tgcaggtcgg ttgcctgccg gtacgtaacc aagccctcgc cgttgagtaa
     2941 gtacagctct tcagggtggc cgtcggcgta gcagactttt ataaagacat cgttgaagaa
     3001 taccaggttc gactcgccgc tgcgtggaat ccaggcaaat atctcgctgg tgccgaaact
     3061 tacatccttg cgcggttcaa agcgcatctg atcacagtcg cagggcaagc tctcgttctg
     3121 ctccttggga ataacgtacg ggcggtgctc caatccccag ccttccctgc cctcggcggc
     3181 acgccgtttg taaatgtcct cgtctatgct gctggcagcg atttttttgg acagtaagag
     3241 ttctaccaat tccttttcag gggtatgagg ataaaagttc gcagccgatg gtacttccgg
     3301 gctcataggt atatattcta acatttttgc tgcgcacggt caaacttagc tgctcttgca
     3361 taaatcggtt gccgattgta caataccgcc cggatcatat ccacttctac ccttggaagg
     3421 ggcagcatgt tcattgaact gagtgtgggc cagccagtcg aactggatat agcgcacacg
     3481 atgctctacg ggggtgtgtt ctcatccgga ggacgcatgt acgcctcgct gaagttcaac
     3541 ttcgtcctgg acgagctgga gcagagccat ctggttgccc tgagcaccca gctgctcggc
     3601 aagcagaccg gcctgccggt tttccagcct ggtccacgcg ttacacaggt actgctgcag
     3661 ttgccggcag gcagcaggca cggcagaacc gacttcctgt gcagggaagt agccaagcgt
     3721 tacggcgagt ggctcataca gcacctgagc gacaaggcga tacgccaaag caaggccagc
     3781 aagaagcacc aaccggccga acagctggcc gtcgtggctt tcctgccgct gcccggagct
     3841 gccttggcta ccaatcccgc tcctgcgagc ccttccagga cgcgacgcgc ggaac
//
LOCUS       DASSFD010000159         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_570305, whole genome shotgun sequence.
ACCESSION   DASSFD010000159 DASSFD010000000
VERSION     DASSFD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570305"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFH39_03935"
     CDS             <1..546
                     /locus_tag="VFH39_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HET6924951.1"
                     /translation="FLAWLPLLLAAWLDVVLRKHRWTSWYGLIVSALWVLFLPNSFYM
                     VSDFIHLQEVPRVDLLYDAVMFTSFIFVGLALGISSLYLVHRQFRRRFSQRLSAVFLC
                     VVFALCGFAIYLGRDLRWNSWDVLTNPGGLLFDISDRILHPSAYSEMFLTITSFGVLI
                     ASMYALVYQAARLVRKLPEQQ"
     gene            567..2234
                     /gene="argS"
                     /locus_tag="VFH39_03940"
     CDS             567..2234
                     /gene="argS"
                     /locus_tag="VFH39_03940"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6924952.1"
                     /translation="MKTELEQAVAVAVKELFGTEVGVDLSRPDEQFGDYATNVALQLA
                     KPVGKNPREIAEALKEKLASGNRLKSIEVAGPGFLNLTLSDEALAAGIGLKPARTLEG
                     KTVLVEYSDPNPFKPLHAGHLYTTLTGDMIARLVETAGAKAVRLNYGGDVGLHVGKSM
                     WAILRSLGGELPDKLDEVLPADRPAWLGARYVEGNDAYEEDEAAKAEIIATNKRVYQL
                     HETGDHDSPFAQIYYTTRQWSYDYFAELYRQLQVKPFDRYIPESEVTPLGVQTVREQQ
                     AQGVFKESDGAVVFAGEEFDLHTRVFINSEGLPTYETKDVGLSLTKWNDYHFDESIII
                     TANEQAQYMQVVITAIRQFAPEPADRTRHLTHGVVKLQGGVKMSSRKGNVVTALEILD
                     AAREAGAQTGTNPSEETILAAVKYAFAKNRIGGDIIYDPKESIALEGNSGPYLQYAHA
                     RARSVLAKAGEQKEAALSELTADERSLLRKITEFTEVVDRAVAELMPHHICTYLYELA
                     QKFNQFYEHNRVIGDEREAGRLQLVRHYADTLKSGLELLGIAAPDHM"
     gene            2275..2514
                     /locus_tag="VFH39_03945"
     CDS             2275..2514
                     /locus_tag="VFH39_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924953.1"
                     /translation="MSVEHSGRAAGNQQLDEQSRLDRRIPGERGLSFVLETDPMYQDL
                     MAKMIEKAQGELQSRLINERTYYKRTGAAGWDVHY"
     gene            2752..3156
                     /gene="msrB"
                     /locus_tag="VFH39_03950"
     CDS             2752..3156
                     /gene="msrB"
                     /locus_tag="VFH39_03950"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET6924954.1"
                     /translation="MQQLTDDEWKDKLTPEQYHVLREKGTEVPGTGSLLYNDADGMYV
                     CAGCGQPLFSSSNKYDSTTPGLIGWPSFSEAADRDAVTLRPDNSMGMHRTEVVCSNCG
                     GHLGHLFDDDSSPNGQHYCINSVALDFKKQND"
     gene            complement(3153..3494)
                     /locus_tag="VFH39_03955"
     CDS             complement(3153..3494)
                     /locus_tag="VFH39_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6924955.1"
                     /translation="MNITDTTPALETRTGCIAAAMDIIGSKWTALILRDLAGGSKRFC
                     ELEKSVGNINPRTLSQRLDDLEAHGIISRKSYAEVPPRIEYSLTKKGKDLIPVLKAMS
                     VWGNKYYDTGC"
     gene            3571..>4036
                     /locus_tag="VFH39_03960"
     CDS             3571..>4036
                     /locus_tag="VFH39_03960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6924956.1"
                     /translation="MKLQIIVGSTRPGRTTPRMAQWIANTAKEIEGVDVEIVDLADYP
                     LPFLDEPISPRFNPNRQPNEQAQRWIDKVAEGDAYVFVTPEYNHSIPGVLKNAIDYLT
                     FEMVHKPATVASHGTVGGARATMHLKEMLSESKAAIIPSQVALAHMSDAIDGS"
BASE COUNT          916 a         1120 c         1184 g          816 t
ORIGIN      
        1 tttctggcct ggttgccgtt actgctcgca gcctggctgg atgtcgtgct gcgcaagcac
       61 cgctggactt cctggtacgg cttgattgtc agcgctctgt gggtgctgtt tttgcccaac
      121 agtttctaca tggtgagcga cttcattcac ttacaggaag taccacgggt ggacctgctg
      181 tacgacgcgg tcatgttcac ctcgttcatc ttcgtcgggc tggcgctcgg gatcagcagc
      241 ctgtatctgg tgcaccggca gttccgccgc cgtttctcgc aacggttgag cgcggtgttc
      301 ctgtgtgtgg tgttcgcgct gtgcggcttc gccatttacc tcggccgtga cctgcgctgg
      361 aatagctggg acgtactgac caaccccggc ggcctgctgt tcgatatatc ggaccgtatt
      421 ctgcacccca gcgcttactc ggagatgttc ctgaccatca cgagtttcgg agtcctcatc
      481 gccagcatgt atgccctggt ctaccaagcg gcccggctgg tacggaagct gcccgaacaa
      541 cagtagttct gctataatta cccctgatga aaacagagtt ggaacaagcg gttgcggtgg
      601 cggtcaaaga attgttcggc accgaggttg gcgtggacct atcgcgccct gatgaacaat
      661 tcggtgatta cgccaccaac gtggctctgc agctggctaa accggtcggt aagaaccccc
      721 gcgaaatcgc cgaggcgctc aaagagaagc tggcgagtgg taaccggctg aaatcgatcg
      781 aggtggctgg tccgggcttt ttgaacctga cattatccga cgaggctctg gccgccggca
      841 tcggcctgaa gccggctcgt acgcttgagg gcaaaacggt gctggtggaa tattccgacc
      901 ccaatccttt caaaccattg cacgccggcc acctttatac cacgctgacc ggcgatatga
      961 tcgcccggct ggtcgagacg gccggtgcga aggcggtacg gttgaactac ggcggtgatg
     1021 tcggcctgca cgtcggcaag agcatgtggg cgattctgcg gagcctcggc ggagaattac
     1081 cggataagtt ggacgaagtg ctgccggccg atcgtccggc ctggctcggc gcccgttacg
     1141 tcgagggcaa cgacgcctac gaagaagacg aggcagccaa ggcggaaatc atcgctacga
     1201 acaaacgcgt gtatcagctg cacgagacag gggaccatga ctcgccgttc gcgcaaattt
     1261 attacaccac ccggcagtgg agctacgact atttcgccga gctgtaccgc cagctgcagg
     1321 tgaagccctt cgaccgctac attcccgaaa gcgaagtgac gccgctgggt gtgcaaaccg
     1381 tgcgcgaaca gcaggcacag ggtgtgttca aagaaagtga tggcgcggtg gtattcgccg
     1441 gtgaggagtt tgacctgcac acccgcgtct tcatcaacag cgaaggtttg ccgacatatg
     1501 agacgaaaga cgtgggcctc agcctgacca aatggaatga ctatcacttc gatgaatcta
     1561 tcatcatcac cgccaatgag caggcgcagt acatgcaggt ggtcatcacg gccatccggc
     1621 agttcgcacc ggaaccggct gaccgcactc ggcatctgac gcacggggtg gtgaagctgc
     1681 agggcggagt gaagatgagc agccgtaaag gtaatgtagt gacagccctg gaaattctcg
     1741 atgctgctcg cgaagccggc gcccagaccg gtacgaaccc gtccgaggag accattttgg
     1801 cggcggtgaa gtatgcgttc gccaagaacc gcatcggggg cgacatcatt tacgacccca
     1861 aggagtcaat tgcgctcgag ggcaacagcg gcccgtactt gcaatacgca catgcccggg
     1921 cgcgtagcgt gctggcgaag gccggcgaac aaaaagaagc cgcactgtcc gaactcactg
     1981 cggacgaacg cagcctgctg cgcaaaatca ctgaatttac agaggtggta gacagggccg
     2041 tcgccgaact gatgccgcac catatctgca cctacttgta cgaactggcc cagaagttca
     2101 accagttcta cgagcacaac cgggtcattg gcgacgagcg agaggcaggg cgcttacagc
     2161 tggtccgtca ttacgctgac acactcaaga gtggcttgga gctgctcggc atcgccgcac
     2221 cggatcatat gtgaccctgg accctcccgg cgggaaagtt tatagtggat agatatgagc
     2281 gtagagcatt ccggtagagc tgccggcaac caacagctgg acgaacagag ccgcctcgat
     2341 agaagaatcc ctggcgagag gggcctttcc ttcgtgttgg aaaccgaccc catgtaccag
     2401 gatttaatgg ccaaaatgat tgagaaggct caaggtgagc tgcagtcacg actgatcaat
     2461 gagcggacgt attacaaaag aaccggcgcc gccggctggg atgtccatta ttaatttccg
     2521 ggtccaattg cttaagccgc ttggctaagc tatgtgatca ggattacaat tataagtgca
     2581 ctaaccgtaa acggagaagg cgacatgaca acattaacca aagactggtg gtggtaggct
     2641 aagccttcac ccgcatacgg cccgcttcgg cgggcttttc ttatttactg ggtttatttc
     2701 ttcacagctc acatacgact gggcgctaca atagagtgag agaaggaact catgcagcaa
     2761 ctaaccgatg acgaatggaa agacaaactg acccccgagc aataccatgt attgcgcgag
     2821 aagggcaccg aggtacccgg caccggcagc ctgttgtata acgatgcgga tggcatgtac
     2881 gtctgtgccg gctgcgggca gccgctgttc agtagtagca ataagtacga ttcgaccaca
     2941 cccggtctga tcggctggcc gagtttctcg gaggcggccg accgcgacgc ggtcacgctt
     3001 cggccagaca acagcatggg catgcaccgc accgaagtcg tctgcagcaa ctgcggaggc
     3061 cacctcggac atttgttcga cgacgattca tcgcccaacg gccaacacta ctgcatcaat
     3121 tcggtggcgc tggacttcaa gaagcagaac gactagcagc cggtgtcgta atatttgttg
     3181 ccccagacgg acatggcttt caggacaggg ataaggtctt tgcctttttt ggtcaggctg
     3241 tattcgatgc ggggcgggac ctcggcgtag cttttgcggc tgatgatgcc gtgggcctcc
     3301 agatcgtcca gccgctggga aagggtacgc gggttgatgt taccgaccga cttttcgagc
     3361 tcgcagaacc gcttcgagcc acctgccaga tcgcgcaata taagcgcagt ccacttgctg
     3421 ccgatgatat ccattgcggc cgcgatgcag ccggtacggg tttccaaagc cggtgtcgta
     3481 tctgtaatgt tcatacttta caaagtatag taatggctct atactggtta attgttaact
     3541 ataaaaaata tagcacatag gagggaattc ttgaagctac aaattatcgt cggcagcacc
     3601 cgcccgggac ggacaacccc gcggatggcc cagtggatcg ccaacaccgc caaggaaata
     3661 gaaggggtcg atgtcgaaat cgtcgatctg gccgattatc cgctgccatt cctcgatgag
     3721 ccgatcagcc cgcgcttcaa cccgaatcgc cagccgaacg agcaagccca acggtggatc
     3781 gacaaagtgg cggaagggga tgcgtacgtc ttcgtgacac cggagtataa ccactccata
     3841 ccgggcgtcc tcaagaacgc catcgattac ctgacgttcg aaatggtgca caagccggcg
     3901 accgtcgctt cgcacggtac cgtcgggggt gcccgcgcca cgatgcacct caaagagatg
     3961 ctgagcgagt ccaaggcagc tatcatcccg tcccaggttg cgttggcaca tatgagtgat
     4021 gcgattgacg ggagtg
//
LOCUS       DASSFD010000160         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1505066, whole genome shotgun sequence.
ACCESSION   DASSFD010000160 DASSFD010000000
VERSION     DASSFD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505066"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..752
                     /locus_tag="VFH39_03965"
     CDS             90..752
                     /locus_tag="VFH39_03965"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6924957.1"
                     /translation="MAKVVPTKPLLIMLYGFPGAGKSYFARQFCEHIQAAHIQADRIR
                     AELFERPRYDKQENEVIAQLMNYMTGEFLNAGISVVYDTNAYRAAQRHALRDMARKAK
                     AQPLLVWLQIDAETAFVRGTKRDRRRADDKYAAQVDRTTFDTVANYMQNPGPGEDYVV
                     ISGKHSFNTQYSAVAKKLRELGLLHLADSHSGIAKPELVNLIPNPAAGRVDMARRNIV
                     IR"
     gene            822..1547
                     /locus_tag="VFH39_03970"
     CDS             822..1547
                     /locus_tag="VFH39_03970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET6924958.1"
                     /translation="MAFKEFRLSPDLPVKVYKRKTSRSLRLSVTAAGEVKVSIPTWTP
                     YAHGLSFANSKLSWIQAQLPAAPPLLQHGQAVGKAHRLFFAASPSAVKPSARLKRTLI
                     VVNYPLASAPDEPLTQTVAARACIRALRTQAGQLLPQRLQALAEQYGFEYSSVSVKLL
                     KSRWGSCDTKQHIVLNLFLMQLPWELIDYVLLHELTHTRVMQHGPKFWGEMERVRPSA
                     KTDRRAMRAYQPILQAAVPLPVA"
     gene            1558..2661
                     /locus_tag="VFH39_03975"
     CDS             1558..2661
                     /locus_tag="VFH39_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6924959.1"
                     /translation="MALKLKKTKASSEQLDHSRLTSLINSMADAVIAVDERVRIVLHN
                     GAALNILDSNTIHEGERLGKLFKPIDKDNQPVDVEKLVLAATTPRTNRDLRLKYDDGS
                     VINLYISIAPVHLGYGEEGQRGFVLLLRDITHEKSLEEERDEFISVVSHELRTPIAIS
                     EGNISNAQLIAEKSGETAQIRQALEEAHNQILYLSDMINDLSTLSRAERGSLNVEIEA
                     INVHDLLTELAANYLPDAKKKGLNIKIELDPKLELLQSGRLYVREVLQNFITNAIKYT
                     QRGHITIGARAKEKGVEFYVSDTGIGINKGDQERVFDKFFRSEDYRTRQNNGTGLGLY
                     VTLKLARLIHADIRLKSKLNHGSTFTIYIPNLQ"
     gene            complement(2653..2877)
                     /locus_tag="VFH39_03980"
     CDS             complement(2653..2877)
                     /locus_tag="VFH39_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924960.1"
                     /translation="MVRRSEIFMGSLGLCLLEAGGALYMAERQPLAGAVYAAIGCIAG
                     IGMTRAIRREGVEPAHVPDTVPENWSDELL"
     gene            complement(2885..>3244)
                     /locus_tag="VFH39_03985"
     CDS             complement(2885..>3244)
                     /locus_tag="VFH39_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924961.1"
                     /translation="LVAFLSLAELSGMLLPLGLLVLAFMRQEWLPALVSALAYVLFTA
                     AWIIIGSMTYGRFTPFSIVSFPLAVLFDVYLMHYSMVQYEFSEVVWKGRNVCIPVMHV
                     VPHMPPLAYRGSHGNAR"
BASE COUNT          800 a          971 c          873 g          600 t
ORIGIN      
        1 gcatcttatc gctggcgcga aggcttgtcg cagaaacagc cgtatttagc ttttcaaatc
       61 tgttaaaacc tgctatacct gtatgtacga tggcaaaggt agtacccact aaaccgctcc
      121 taatcatgct ttacggcttc ccgggagcgg gcaaaagcta ctttgcacgc caattctgcg
      181 aacacatcca ggctgcccat attcaggccg atcgcatccg ggccgaactg tttgagcgcc
      241 cccgttacga taagcaggaa aacgaagtta tcgctcagct catgaactat atgacaggcg
      301 aattcctgaa cgccggtatc agcgtggtat acgacaccaa tgcctaccgg gccgcccagc
      361 ggcacgccct gcgcgacatg gcacgcaaag ccaaggcaca accgctgctg gtctggctgc
      421 aaatcgacgc cgaaacggcc tttgtgcgcg gtacgaaacg cgaccggcgc cgggccgacg
      481 acaaatacgc ggcccaggtg gaccgcacta cttttgatac cgttgcaaac tatatgcaga
      541 accccggtcc cggcgaagat tatgtggtta tcagcggcaa gcactcgttc aacacgcagt
      601 acagcgccgt ggcgaagaaa ttgcgtgagc tggggctttt gcatctggcc gattcgcaca
      661 gcggcatcgc caagccggag cttgtgaacc ttattccgaa cccggctgcc ggccgtgtcg
      721 acatggcgcg ccgcaacatc gttatacgct aataaacagc ctgcagccgc ggcagtggca
      781 ataaacctaa acagctaacg gctaactgct aaaatatatg tatggctttc aaagaattcc
      841 gcctaagccc ggacctgccc gtcaaagtat acaaacgcaa aaccagccgt tcgctgcgcc
      901 ttagcgttac ggctgccggc gaagtcaaag tcagcatacc tacctggacg ccctacgcgc
      961 acggcctgtc gttcgcgaac tccaaattga gctggataca agcccagctg ccggcagccc
     1021 cgcccctcct gcagcacggt caggccgtcg gcaaagcgca ccgtctcttc tttgccgcca
     1081 gcccctcggc cgtaaagccg tcagcgcggc tgaagcgcac cttgatagtc gtgaattatc
     1141 cgcttgcttc cgcaccggat gagccgctga cgcaaacggt ggccgccaga gcctgcattc
     1201 gggcgctgcg cacgcaggcc ggtcagcttt tgccgcagcg cttgcaggcc ttggcagagc
     1261 aatacggttt cgaatacagt tcggtcagcg tcaaactgct gaaaagccgg tggggcagct
     1321 gcgacacgaa acagcacatt gtcctaaatc tgttccttat gcaactgccg tgggagctga
     1381 tagattacgt gttgttgcac gagcttacgc acacccgtgt catgcagcac ggcccgaagt
     1441 tctggggtga aatggaacgg gtgcggccca gtgccaagac cgaccggagg gctatgcgcg
     1501 cctatcagcc gattttgcaa gctgccgtac cgctacctgt ggcataatgg gactgtcatg
     1561 gcgcttaagc tcaaaaaaac caaagcttcc agcgagcagc tggatcatag ccgactcacc
     1621 agcctcatca acagcatggc cgatgcggtc atcgccgtag atgaacgggt aagaatagtg
     1681 ctgcataacg gcgcggcgct caatatactc gacagcaaca ctattcacga aggcgagcgc
     1741 ctgggcaagc tgtttaaacc aatcgacaag gacaaccagc cggtggatgt cgagaaactg
     1801 gtactcgcgg caaccacgcc ccggacaaac cgtgatctgc gcctgaaata cgatgacggc
     1861 agcgttataa acctgtatat ctcaatcgcg ccggtacatc tgggctatgg cgaggaaggg
     1921 cagcgcggtt tcgtactgtt gctgcgcgat atcacccatg aaaagtctct ggaagaggag
     1981 cgcgacgagt tcataagcgt cgtcagccac gagttgcgca cgcccatcgc gataagcgag
     2041 ggcaatatta gcaacgctca gctcatagcc gaaaaaagcg gcgaaaccgc gcagatccgg
     2101 caggcgcttg aggaagccca caatcagata ctttatctct ccgacatgat aaacgacctg
     2161 tccactctgt cccgcgccga gcgcggcagc ctgaatgtcg aaatcgaagc tatcaacgta
     2221 cacgatctgc ttaccgaact ggccgctaac tacctgccgg acgcgaagaa aaaagggctg
     2281 aacataaaga tcgagctgga ccccaaactc gagctgctgc aatccggccg tttgtatgtc
     2341 cgcgaagtcc tgcaaaactt tataaccaac gccataaagt acacccagcg cggtcacatt
     2401 accataggcg cgcgggcgaa ggaaaagggt gtggaatttt atgtttccga cactggcata
     2461 ggcatcaata agggagacca ggaacgggtg ttcgacaaat ttttccgcag cgaagactac
     2521 cgtacccgcc agaataacgg cacggggctg ggcctgtacg ttaccctcaa gctggcccgg
     2581 ctcatccacg ccgatattcg gctcaagagc aagctgaacc acggctccac cttcaccatt
     2641 tacataccca acttacaata gttcgtcaga ccagttttct ggcaccgtat cgggaacatg
     2701 tgccggttcg actccttcgc ggcggatggc acgggtcatc cctatgccgg cgatacagcc
     2761 gatggccgcg tacacggcgc cggccagcgg ctgccgttcg gccatgtaca gcgcaccgcc
     2821 ggcttccagc aggcacagcc ccaagctgcc catgaagatc tcggaacggc gaaccatgcg
     2881 ctctctagcg ggcatttcca tggctacccc ggtaagcaag cggcggcatg tgcggtacga
     2941 catgcataac gggtatgcag acgttgcggc ccttccagac gacttcgcta aattcgtact
     3001 gaaccatcga gtagtgcatg aggtagacgt cgaacagtac ggcgagcgga aagctgacaa
     3061 tgctgaatgg cgtaaagcgg ccatacgtca tgctgccgat gatgatccag gcggccgtga
     3121 acaaaacata agccagcgcg cttaccaggg ccggcaacca ttcctgacgc ataaaggcca
     3181 gcaccagcag tccgagaggc aacagcatgc cgctgagctc tgccaggctc aaaaacgcta
     3241 ccaa
//
LOCUS       DASSFD010000161         5284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1078493, whole genome shotgun sequence.
ACCESSION   DASSFD010000161 DASSFD010000000
VERSION     DASSFD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078493"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2611)
                     /locus_tag="VFH39_03990"
     CDS             complement(<1..2611)
                     /locus_tag="VFH39_03990"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6924962.1"
                     /translation="MQLPKTYEPNEYEKDIYDLWEQSRVFVADPHSKKPHFSITMPPP
                     NETGTLSIGHSLFLTLQDIMSRYQRAKGKDVLWLPGTDHAALAVNAIIEKKLAEEGTD
                     KHAVGRDEFLRRTREFVQGSRGTMLGQMRAMGASADWTRLHNTLDAAPSRAVAEVFVK
                     MYNDGLIYRGHRIVNWDPNLETTVSDDEVVYTEEKTKFYTFKYGPFEIGTARPETKFG
                     DKYVVMHPEDERYKQYQHGDTFEAEWINGTVTATVIKDESIDPNFGTGVMTITPWHSH
                     VDFEIAERHGLDREQIIDFHGKLLPIAGEFAGMDIAEARPKIVEKLAAKGLLVKTDEN
                     YVHNVAVNERGKGTIEPQIRLQWFVDVNKPAVEWKGQQRSLKEVLQSVIRDGDITIVP
                     ERFNKIYFHWIDNLRDWCVSRQIWWGHRIPVWYRQDTDGREETYVGVTPPTDESEGWH
                     EWEQDPDTLDTWFSSALWTWSTLIDPALAEDYSLSLEDLLAQSPDFQAYHPTSVLETG
                     WDILFFWVARMILSTTYATGQVPFKTVYLHGLIRAEDGKKMSKSRPESIIDPLEVIPK
                     YGTDALRMALIMGVSPGNDQNWGWGKIEANRNFCNKLWNIARYIEGLVGDDYRPKDAK
                     PQTTADHWVLSKLQRITEDIAADLEAYRFGEAYDKLYHFVWDDFADWYVEASKAQPNL
                     PLLAHCLESILIIMHPFAPFVTETIWQTLGWEGDSVLAARPWPTIKAGDPAKATEFEE
                     IQRIVSECRLITKALGVGGVTLFYKKSELLRTNAELIARLARLQAVTVALEGSAEQGI
                     ALTGTGKAAWLDIAPEVAQGYAARLQERISHQEKVIAQLEGRLQNDSYVQNAPAEIVE
                     QTRQQLRDAQAQLA"
     gene            complement(2694..3755)
                     /gene="tsaD"
                     /locus_tag="VFH39_03995"
     CDS             complement(2694..3755)
                     /gene="tsaD"
                     /locus_tag="VFH39_03995"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET6924963.1"
                     /translation="MIVLGIETSCDETAAAVGGDGCELLSNVVASSMDLHKQYGGVVP
                     EIAARSHIEVILPVVQQALDDAQCKWDDIDAIAVTQGAGLGGSLLIGVLTARTLAIAY
                     GKPLYAANHVMGHVFANFLTATSLPGYAMRSAQPTFPMLAIIVSGGHSQLALFRDYFD
                     YSLLGQTQDDAIGEAFDKVAKITGLPYPGGPSIAKAAANGNAHAFELPKAKMAGKYDF
                     SFSGLKTAVLRQAQAMIGADYTFPSKDLPERLSEAQKADIAATFQRVAVETIVDKAVM
                     ATAEYSPASVVIAGGVAASGELRRQLTERLPSPIEFPDLKLCTDNGAMIATLGCYMAS
                     EGQPTADPYTLDIAPNLSM"
     gene            4150..5244
                     /locus_tag="VFH39_04000"
     CDS             4150..5244
                     /locus_tag="VFH39_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924964.1"
                     /translation="MAREIGLQLDNHGHAHYESGVEVEAVEQPDGTVRQVAKRTKTGK
                     PRSIRGRLMSRHELEMIAAHLDVIRDYQDERAAEYAAWHDAGRNILYGVEHERIWGRR
                     VATIRDGTMQEKNTAATIPALPEQADHPKKQVQSQADGQDENTDQPPLVWKDDYIAAF
                     LPPSMTREYYTPPPAPDQRPGFYRRHELGIKAAAVAGTLALAATMLGLTSANDEHAPP
                     ASHVHIETQPGPGAIQEPMPGYGEGRVGQRHVPSQGQKSKEQPRHHAASVVVPTSITL
                     NGHEGDSLWSASARSITLNSEGLYKALGHQRADELLVRVTNQEMRDNGIPNERAAEHL
                     PVNFSARISKQVKRIVRHFVHRFIKARARA"
BASE COUNT         1051 a         1579 c         1523 g         1131 t
ORIGIN      
        1 tggccagttg tgcctgcgcg tcgcggagct gctgccgcgt ctgctcgacg atttctgccg
       61 gtgcattttg gacgtaggag tcgttctgca gcctgccctc cagctgggcg ataacctttt
      121 cctgatggct gatgcgttcc tgtaagcggg cggcatagcc ttgggctacc tctggggcta
      181 tatcgagcca ggcggctttg ccggtgccgg tcagggcaat cccctgctcc gctgagcctt
      241 ccagggcgac ggtgacggct tgtaggcgcg ccaaccgggc aatcagctcg gcattggtcc
      301 tcagtagttc cgacttctta taaaacagcg tgacgcctcc cacgccgagt gccttggtaa
      361 ttagccgaca ctcgctgacg atgcgctgaa tttcttcgaa ctcggtcgct ttggcaggat
      421 cgccggcctt gatggtcggc caggggcgag ccgccagtac gctatcccct tcccagccca
      481 gcgtctgcca gatggtctca gtcacgaacg gggcgaacgg gtgcatgatt atcaaaatac
      541 tttccaggca gtgagccagc agtggcaggt tcggctgggc cttgctggcc tccacatacc
      601 agtcggcgaa gtcgtcccac acgaagtggt acagcttgtc gtaggcttca ccgaagcggt
      661 acgcctccaa atcggcggcg atatcttcgg taattcgttg caatttagac agcacccaat
      721 gatcggcagt ggtttgcggc ttagcgtcct tcgggcggta atcgtcaccg accagccctt
      781 ctatgtagcg ggcgatgttc cacagcttgt tgcagaagtt ccggttggcc tcgatcttgc
      841 cccaacccca gttctgatcg ttgcccgggc tgacgcccat gatcagcgcc atgcgtaacg
      901 cgtcggtgcc gtacttcggt atcacctcca gcgggtcgat gatgctttcc ggccggctct
      961 tgctcatctt cttgccgtcc tcagcccgga tcagaccgtg cagatatacc gtcttaaacg
     1021 gcacctgccc ggtggcgtag gtggtgctca gtatcatccg ggccacccag aagaatagaa
     1081 tgtcccagcc ggtctccagc acgctggtcg gatgataggc ctgaaaatcc gggctttggg
     1141 ctagcagatc ctcgagcgac agcgaataat cctcggccaa ggcagggtcg atgagcgtgc
     1201 tccaggtcca cagcgcgctg ctgaaccagg tgtccagcgt atccgggtcc tgctcccact
     1261 cgtgccatcc ttcgctttca tccgtcggcg gcgtgacgcc cacgtacgtc tcctcgcggc
     1321 cgtccgtatc ctggcggtac cacaccggta tgcggtggcc ccaccagatc tgccggctga
     1381 cgcaccagtc ccgcagatta tctatccagt ggaaatagat cttgttgaaa cgctcaggaa
     1441 cgatggtgat gtcgccgtca cggatgacgc tctgcagcac ttccttcagg ctgcgctgct
     1501 ggcctttcca ctccacggct ggcttgttga catcgacgaa ccactgcagc cggatctgcg
     1561 gctcgatagt tcccttccct cgctcgttca ccgccacgtt gtggacgtaa ttctcatctg
     1621 ttttgaccag cagacctttg gctgccagct tctcaactat cttgggccgc gcttcggcaa
     1681 tatccatgcc ggcgaactcc ccggctatcg gcagcagctt cccgtgaaag tcgatgatct
     1741 gctcgcggtc aagcccgtgc cgctcggcaa tctcaaagtc gacatggcta tgccacggcg
     1801 tgatggtcat aaccccggta ccgaagttcg ggtcgattga ttcgtccttg atgacggtag
     1861 cggtaacggt gccgttgatc cactccgctt cgaaggtatc gccgtgctgg tactgtttgt
     1921 aacgctcgtc ctcagggtgc atcacgacgt acttgtcgcc gaatttggtt tcaggccggg
     1981 cggtaccgat ttcgaacgga ccgtatttga aggtataaaa cttggtcttt tcttcggtgt
     2041 agacgacttc gtcatccgag acggtcgtct ctaggtttgg gtcccagttg acaatgcggt
     2101 gacctctgta aatcagtccg tcgttataca tttttacgaa cacttcggct accgctcggc
     2161 taggggcggc atctaaggtg ttgtgcaggc gggtccagtc agcgctggcg cccatagcgc
     2221 gcatctggcc cagcatggtg ccgcggcttc cctggacgaa ctcccgggtg cggcgcagaa
     2281 actcgtcccg gccgacggcg tgcttgtcgg tcccctcctc tgccagcttc ttctcgatga
     2341 tggcgttcac cgccagcgcc gcgtggtccg taccgggtag ccacagcacg tccttccctt
     2401 tggcccgctg atagcggctc atgatgtctt gcaaggtcaa aaacagcgag tggccgatgc
     2461 tcagcgtccc cgtttcattc ggtggcggca tggtgatcga gaagtgtggc tttttgctgt
     2521 gcgggtccgc cacgaacacc cggctctgtt cccagaggtc gtagatgtcc ttttcgtact
     2581 cgttcggctc gtaggtcttc ggcagttgca taattcacct cttatagtac cactgaaccg
     2641 cccctgttac tagcgtaccg cttcatgtga aacggagcgg catgcgtaat actttacatg
     2701 ctaaggttcg gtgcgatatc gagggtgtac gggtcggctg tcggctgccc ttcgctggcc
     2761 atgtaacagc cgagcgtggc gatcatggcg ccgttgtcgg tgcagagttt gaggtcgggg
     2821 aactcgatag gtgaggggag ccgttcggtc aactgccgac gcagttcacc gctggcagcc
     2881 accccgccgg ctataaccac cgaggcaggg gagtactccg cggtcgccat gaccgcctta
     2941 tccacgatgg tttctacggc aacacgctgg aacgtagcgg caatgtcggc cttctgagcc
     3001 tcagagagcc gttctggcag gtctttggag ggaaatgtgt aatccgcacc gatcatggcc
     3061 tgggcctgtc gcagaacggc cgttttgagg ccggaaaagc tgaagtcgta tttgccggcc
     3121 attttggcct tcggtagctc gaaggcgtga gcgttgccgt tggctgccgc tttcgcaatc
     3181 gacgggccgc cagggtaggg caggccggtt atcttggcga ccttgtcgaa cgcctcaccg
     3241 atggcatcat cctgggtctg ccccagcaag ctgtaatcaa agtagtcacg gaacagggcc
     3301 agctgactgt ggccgccgct gacgatgatg gccaacatcg gaaaggtagg ctgggccgag
     3361 cgcatcgcgt agccgggcag ggaagtagcc gtcaagaaat tggcgaagac gtgccccatg
     3421 acgtggttgg ctgcatacag cggtttgccg taggcgatag ccagggtgcg ggccgtcaaa
     3481 accccgatca gcaggctgcc accgaggccg gcgccctggg tcaccgctat ggcgtcgata
     3541 tcatcccact tgcactgggc gtcatccagc gcctgctgca ccaccggtag gatcacctct
     3601 atatggctgc gcgccgcgat ttccggcacg acgcccccgt actgcttatg caggtccatg
     3661 ctgctcgcca ccacgttgct cagcaactcg cagccatcac cccctaccgc ggcagccgtc
     3721 tcgtcgcaac tcgtctcaat ccctagaacg atcatcgcac ctagtgtaac aaatcatata
     3781 gagacgtatc gcggaaaggg ttacatactt aggtgtgcga ccgtatagga tagaaagcaa
     3841 gggaagcata tggtaagagt tgctgtttcg tctgaacaac ccacagaatc atctataaac
     3901 tctccggtag atttcgcaaa gccctcgctc acagccgtgg aagatgcgtc tcggccgcaa
     3961 cctgacgata ctgccgatcg accggtcgac tcagtgtatg tacacaggtg tcaaaaactc
     4021 ctggaatggc aaacaccggc tgctatacgt gaacaagagc aatccgcccc gcccgttccg
     4081 gttattcgta gtctagaaga agcgccgacc gattcggcag agaagcgtcg gcaagcaatc
     4141 ggtcatctgt tggcacggga aatcggcctg cagctggata atcatggcca cgcccactac
     4201 gaaagcggcg tagaggtaga ggctgttgag cagcccgatg ggactgtccg gcaggtcgcc
     4261 aagcgtacaa aaacaggtaa gccccgctca atccgcggcc gattgatgag ccgccatgaa
     4321 ctggaaatga tcgcggccca cctggatgtc ataagagact atcaggacga acgggcggct
     4381 gaatacgcag cctggcacga cgcaggccgt aatattctct atggtgtcga gcacgagaga
     4441 atctggggca ggcgagtggc gactatcagg gacgggacaa tgcaagaaaa aaacactgcc
     4501 gctactattc cggcactgcc tgagcaagcc gaccatccca agaagcaggt gcagtcccag
     4561 gcagatggcc aggacgagaa tacggaccag cctccactcg tctggaagga tgactatatc
     4621 gccgccttcc tcccgccgag catgacgcgt gagtactaca cccctccgcc tgcccccgat
     4681 cagcgccctg gtttttatcg tcgccatgaa ctgggcatca aagctgcagc ggttgcgggc
     4741 acactcgcct tggcggcaac catgctaggc ttgacctccg ccaacgatga gcatgctccc
     4801 cccgccagtc atgtacacat agagacacag ccggggcctg gggcgatcca ggaaccaatg
     4861 ccagggtatg gtgagggtcg ggtgggccaa cggcatgtgc caagccaagg ccaaaagagc
     4921 aaggagcaac ctcggcatca tgctgcatcg gtggttgtgc ccactagcat caccttaaac
     4981 ggtcacgaag gagatagcct atggtcagcc tccgctcgca gcattaccct caacagcgag
     5041 gggctctaca aagctttggg ccatcagcgt gccgatgaat tacttgtccg cgtcacaaac
     5101 caagagatgc gcgataacgg catacctaac gagcgcgccg ccgagcatct tcccgttaat
     5161 ttctcagctc gaatttctaa gcaggtgaaa agaatagtac ggcacttcgt gcaccggttc
     5221 atcaaggctc gtgctcgggc ttaacctggt gaaggattac gaatatctaa atattgacgc
     5281 aggc
//
LOCUS       DASSFD010000162         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1843841, whole genome shotgun sequence.
ACCESSION   DASSFD010000162 DASSFD010000000
VERSION     DASSFD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843841"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..950)
                     /locus_tag="VFH39_04005"
     CDS             complement(27..950)
                     /locus_tag="VFH39_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET6924965.1"
                     /translation="MITLLQAIILGLVQGVTELFPVSSLGHAVIIANLFNWNNLLKQE
                     TADKSGFLTFLVVLHVATAIALIIFYRREWVRIVRGLIRSIQARDLGLDSDARLGWLL
                     IAATIPAGIIGLLFEHLLRAEFAKPIFAIIFITINGILLIKGDKVVKAAQRQRPRQRG
                     RAAESEGPSMAQTATMVSDHLTIGRAMIIGVAQIGALFAGISRSGITMLAGLRSGLNH
                     EDAARFSFLLATPIILAAGLYKLPDVVGPSGNGIRGQALIGGLVAGIAAYFSVKYLDK
                     YFQTRSLRPFGIYCVILGVCMFLLGIFRGHF"
     gene            complement(970..2364)
                     /gene="zwf"
                     /locus_tag="VFH39_04010"
     CDS             complement(970..2364)
                     /gene="zwf"
                     /locus_tag="VFH39_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6924966.1"
                     /translation="MNDQGAAQLEPSVIVVFGITGDLAGRYLLPALYHLFQAGLLNPQ
                     TEILGLTRQDLTVEDVFGRIKNGITEKDGSCDQAVLKRMQEHSRLLQFDPSQAADYGK
                     LRDELERLETEQGRCLHRLYYLSIPPDLFETTVQNLGSAGLNGSCSHGAASSRLLVEK
                     PFGSDLASAQALIDATNRVFREEQVFRIDHYLAKETVQNILWFRASNPIFSAVWDNQH
                     ITSIEIQAKEKIGIEGRKVFYDKVGALRDLIQSHLLQLLAVTTMSLPAELTSESIHLH
                     KQSLLESVVPADPSQAVRGQYEGYREEAEATDSATETYARLTLNIDTSEWRDVPVTIT
                     TGKALDEKRTDITVDFTDRSNPTNEVNRLVFRIQPDEGIHVQLRVKRPGLDNELQSAA
                     LDFSYQQTFGDGSQPDAYERVLVDAVKGDRTLFATSAEVLASWRIIQPVIDAWRDNAD
                     GLQTYARGSSGPAA"
     gene            complement(2361..>2577)
                     /locus_tag="VFH39_04015"
     CDS             complement(2361..>2577)
                     /locus_tag="VFH39_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924967.1"
                     /translation="ENPELSGIDGYVAENGEGRWTFETAQAAGIETPVLQASLEVRAA
                     SREGSVSFATKLLAAIRNKFGGHELNK"
BASE COUNT          490 a          724 c          820 g          543 t
ORIGIN      
        1 tgccgctgca ctgagaaatt cgccacttag aaatgcccgc ggaagatgcc cagcaggaac
       61 atgcagacac caaggatgac gcagtagata ccgaacggcc gcagactgcg ggtctgaaag
      121 tatttatcga ggtacttgac agagaagtag gccgcgatgc cggctaccag tccgccgatc
      181 agcgcctggc cacggatacc gttgccgctg ggccccacga cgtccggcag tttgtataag
      241 ccggccgcca gtatgatcgg cgtcgccagc aaaaaggaaa agcgggccgc atcctcgtgg
      301 ttcaagccgc tgcgcagtcc ggccagcatg gtgatgccgg agcggctgat gccggcaaag
      361 agcgcgccga tctgggccac accgataatc atcgcccggc cgatggtcag gtgatcgctg
      421 accatggtgg ccgtctgggc catggaagga ccctcgcttt cggccgccct acctctttgg
      481 cgggggcgct gccgttgagc ggccttgacg actttgtcgc cctttatgag taagataccg
      541 ttgatggtaa tgaagatgat ggcaaaaatc ggtttggcga attcggcccg cagcaagtgt
      601 tcaaacagca gcccgatgat accggccgga atcgtcgccg cgatcagcag ccaaccgagc
      661 cgggcgtcgc tgtccaggcc caggtcgcgg gcttgtatgc tgcggataag cccccggacg
      721 atgcgtaccc attcgcgccg gtaaaaaata atcagggcga tggcggtggc cacatgcagg
      781 acgaccaaaa atgtcaggaa gccggacttg tcggccgtct cttgcttgag caggttgttc
      841 caattgaata ggttggcaat gatgacggcg tgccccaggc tggacaccgg aaacagttcg
      901 gtcacgccct ggaccagacc caggataata gcttgcagca gcgttatcat cggaaattat
      961 tgtaccagat tatgcggccg gtccgctgct gccgcgggcg taggtttgca ggccgtccgc
     1021 gttatcgcgc caggcgtcga tgaccggttg gatgatgcgc cacgaggcta gcacctcggc
     1081 gctggtcgcg aacaaggtac ggtctccctt gacggcgtca accagcaccc gttcgtaggc
     1141 gtccggttgg ctgccgtcac cgaaagtctg ctggtagctg aaatcgaggg cggcggattg
     1201 cagctcgttg tccaggcccg gccgtttgac gcgcagctgc acatggatgc cctcgtcagg
     1261 ctggatacgg aacactaggc ggtttacttc gttcgtcgga ttgctgcgat cggtgaaatc
     1321 taccgttatg tcggtgcgtt tctcatctag tgccttgccg gtggtgatgg tcaccggtac
     1381 gtcacgccac tcgctggtgt cgatattgag ggtcaaacgg gcgtacgttt cggtggccga
     1441 atcggtcgcc tctgcctctt cgcggtaacc ttcgtattgc ccgcgcacgg cttgggacgg
     1501 gtcagccggc acgacagatt cgagtaacga ttgcttatgc aagtggatgc tttcgctggt
     1561 cagctcagcc ggcagcgaca tggtggtgac ggccaacagc tgcaacagat gactttggat
     1621 aagatcgcgc agtgccccga ctttgtcgta aaagactttg cggccttcga tgccgatctt
     1681 ttccttggcc tggatttcta tggacgtgat gtgctggtta tcccagacgg ccgaaaagat
     1741 ggggttgctg gcgcggaacc ataagatgtt ctgcacagtt tccttggcca agtagtggtc
     1801 gatacggaac acctgctcct cgcggaagac gcggttggtg gcgtctatga gcgcttgggc
     1861 gctggccagg tccgacccga acggcttctc gaccagcagc cggctggacg ctgcaccgtg
     1921 gctgcagctg ccgttgaggc cggcgctgcc gaggttctgc accgtggtct cgaataagtc
     1981 gggtggaatc gacaggtagt acaaccggtg caggcacctg ccctgttcgg tttcgaggcg
     2041 ttcgagctca tcgcggagtt ttccgtagtc ggcagcttgc gacggatcga attggagcaa
     2101 ccggctgtgc tcctgcatcc gtttgagcac ggcttggtca catgatccgt ccttctcggt
     2161 gatgccgttc ttgatgcggc cgaagacatc ttcgacggtc agatcctggc gcgtcaggcc
     2221 caggatttcg gtttgcgggt tgagcaggcc ggcttggaac agatggtaca gcgccggcag
     2281 caaataacgg ccggccaggt cgccggttat gccgaagacg acgatgacag acggttcgag
     2341 ctgagcggcg ccttggtcat tcatttgttc aactcatggc caccgaattt gttgcggatg
     2401 gcggccagta atttggtggc gaaactgacg gaaccctcac gcgaagcagc ccggacttcc
     2461 agtgaggctt ggagtacggg ggtttcgatg cccgccgcct gggcggtttc gaacgtccac
     2521 cggccttcgc cgttctcggc gacgtagcca tcgataccgg agagttccgg attctct
//
LOCUS       DASSFD010000163         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_591324, whole genome shotgun sequence.
ACCESSION   DASSFD010000163 DASSFD010000000
VERSION     DASSFD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591324"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFH39_04020"
     CDS             complement(<1..549)
                     /locus_tag="VFH39_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6924968.1"
                     /translation="MDKAVKINNLSVTLGGTVEALTDVNLELPAGKTVGFIGPSGAGK
                     TTLIRAIVGRLQVPAGTVIVFGEEAGSKRLRSQLSYMTQALSLYEDLTAAQNLKFFAS
                     MCGVNTKHVKDAVEETLKTVELTDRSGSLVRDLSGGQRQRVSLAVALVAKPKLLVLDE
                     PTVGLDPVLRVELWEMFHALAAA"
     gene            complement(724..978)
                     /locus_tag="VFH39_04025"
     CDS             complement(724..978)
                     /locus_tag="VFH39_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924969.1"
                     /translation="MLSQYTRTAVTQRLLDGEVREKLEAMENDPTLNTRASSYSANSE
                     LYPNGQIPFADKHIAYLMDHPKVNHAQYLSNLRLMLKVRG"
     gene            complement(1129..2355)
                     /locus_tag="VFH39_04030"
     CDS             complement(1129..2355)
                     /locus_tag="VFH39_04030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6924970.1"
                     /translation="MPYKSKTYSSRRFGGNYHGGGASSYSSSGRSRSSGGSKGPKKDY
                     IDPARFVKVAQPTEAMEYTPLHEFVDFDVNLLIKANLDAKGYKTPTPIQDKTIPAALA
                     GRDIIGIADTGTGKTAAFAIPLLQAMMLGDARALVIAPTRELAQQIDAEFRWIAKGSN
                     LKGATLIGGAGIGPQLRDLRANPQIVIGTPGRIKDHLERGSLRLDNFTFVVLDEVDRM
                     LDMGFVNDVRNILNRVSAKRQSFFFSATMDPKVNALIETFASDPITISIKTGDTGENI
                     HQDVVRVGHDSEKMDKLHDLLISGELRKVIIFDDTQRSVERLSKELISRGFSADAIHG
                     GKTQGARQRSLKKFKDNEVKILVATDVAARGIDVAGITHVINYSTPKSYADYVHRIGR
                     AGRAGKIGYALTFITQ"
BASE COUNT          564 a          800 c          751 g          702 t
ORIGIN      
        1 ggcggcggcg agggcgtgga acatctccca cagctccacg cgcagcaccg ggtccaggcc
       61 gacggtcggc tcgtccagta ccagcaattt cggcttggcc actaacgcca ctgccaggga
      121 aacgcgttgc cgctggccgc ccgacaggtc gcgcaccagg ctgccggacc tatcggtgag
      181 ctccactgtt tttagcgttt cctcgacggc atccttaaca tgtttagtat tcacgccgca
      241 catgctggca aaaaacttca ggttctgcgc cgcggttagg tcctcgtaga gggaaagcgc
      301 ttgcgtcata taactcaatt ggctgcgcag ccgtttcgag ccggcctcct caccgaacac
      361 gattaccgtg cctgccggca cctgtaatcg ccccacgatg gcccttataa gagtggtctt
      421 ccccgcgccg cttggcccta tgaaccccac cgtcttacct gccggcagct ccaaattcac
      481 atccgtgagc gcttcgaccg tcccgcccaa agtaaccgac aaattgttta ttttaacagc
      541 cttatccatg ccttcatgat agcaaaagta aagtcatagt cggaactaac ggataactta
      601 aaacagctgc aggctggacg tgatccgctt ttccagcacc agccagccgc ccagcttcac
      661 tccccgtatc ggttccgtct tcatgttgcc aatccagcaa ctccgccggc acccggggcc
      721 gtcctagccg cggactttaa gcatcaggcg caggttcgac aggtactgcg cgtgatttac
      781 tttcggatga tccataagat acgctatgtg cttgtcggcg aaagggatct ggccattcgg
      841 atacagttcg ctgttggcgc tgtaactgct ggcccgggtg ttcagggtag gatcgttttc
      901 catggcttcc agcttttcgc ggacttcacc gtccagcagc cgctgggtta ctgccgtacg
      961 cgtgtactga ctgagcatgc ttacgacttt gcctgcttag gttttttgat ttctttgcgt
     1021 cctttgtcct tagacatctg gcggccccct ctttcaggta agttttttgt aactctttgt
     1081 cttaactata cgccgaaagg tccgattcct ctagtacata cttgtgtttt actgcgttat
     1141 gaacgtcagc gcgtagccga tcttgccggc ccggccggcg cgccctatac ggtgtacgta
     1201 gtcggcgtat gacttcggag tggagtaatt gatgacatgc gttatgccgg ccacatcgat
     1261 gccacgggca gccacgtcgg tcgccaccag tattttcact tcgttgtctt tgaacttttt
     1321 gagcgagcgc tggcgggctc cttgggtctt gccgccgtgt atggcatcgg cgctaaagcc
     1381 gcggctaatg agttctttgg acagccgttc cacgctcctt tgcgtatcgt caaagatgat
     1441 cactttgcgc agttcgccgc ttatcagcag gtcgtgaagc ttatccattt tttcgctgtc
     1501 gtgccctacg cgcaccacgt cctggtgaat attttcgccg gtgtcgccgg tttttatgga
     1561 aatggtgatc ggatcgctcg cgaatgtttc aatcagagcg ttcactttgg ggtccatagt
     1621 tgcggagaaa aagaatgact ggcgcttcgc gctcacccga ttcaatatgt tgcgcacatc
     1681 gttcacgaaa cccatgtcca gcatacggtc gacttcgtcg agcactacaa aagtaaaatt
     1741 atctaaccgt aggctgccgc gctccagatg gtctttgatg cggcccggcg taccgataac
     1801 tatctgcggg ttcgcccgca ggtcgcgcag ttgcggcccg atcccggcgc cgccgatgag
     1861 cgtcgcacct ttcaggttgc tgcccttggc tatccaccgg aactcggcgt cgatttgctg
     1921 cgccagctcg cgcgtcggcg caatcaccag cgcccgggcg tcgcccaaca tcatggcctg
     1981 caacaggggt atggcaaaag ccgctgtttt gccggtaccg gtgtcggcga taccgattat
     2041 gtcgcggccg gcgagcgcgg ccggaatagt tttatcctga atgggcgtcg gcgtcttgta
     2101 gcccttggcg tccaggttgg cttttatgag cagattgacg tcgaagtcca cgaactcgtg
     2161 cagcggcgtg tactccatgg cttccgtggg ctgcgccact tttacgaagc gcgccgggtc
     2221 tatgtagtct ttctttggtc ctttgctgcc gccggaactg cggctgcggc ccgaagagct
     2281 gtaagagctc gcgcctccgc catgatagtt gccgccaaaa cggcggctgg aatacgtttt
     2341 gcttttataa ggcataaaaa tagaatcctt gctgttagtt actctttact gcgattgctg
     2401 aaatcgagta aaaattgcct aacccggatt catcaaaaac aatacaacta cattagcacg
     2461 atacggtaag aattgcaagg taacaaaaag cgccgcctaa tgttgatgcg cttttggtgg
     2521 agctggtggg tactgccccc acgtccgtcg gcttatctga ggttagtctt ctacaggcat
     2581 agaccgttca agtgtcttag gcagttggat tagagcggac aagaaaccaa ctgcggcagt
     2641 ccaaagtctt agcgaccagc caggacagtt ctagtcgagc gtccagaaga tataaaacgc
     2701 ttctgcgcta tctgaagtca cgcatggcgc tgctttcgga tattaagcga aagctggtgc
     2761 gaaaaccggc atgcggaaca tgctggcaac gcggttcaaa acttttgcag ttattga
//
LOCUS       DASSFD010000164         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1709212, whole genome shotgun sequence.
ACCESSION   DASSFD010000164 DASSFD010000000
VERSION     DASSFD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709212"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VFH39_04035"
     CDS             <1..1000
                     /locus_tag="VFH39_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HET6924971.1"
                     /translation="LIPLVRGQRLAVLGDSKTGKSTLAMQLTLNQKDSDVVVVYVLIA
                     KRKSDVDQLLSRLQEEDALKSAIVVVATMFESLIMSYLAPYVGCALAEYLWQKGDTDV
                     VVVYDDLTSHAHAYREISLLAGVSPGRDSYPGDMFFAHSSLLERAGRLKANHKTLTAL
                     PLVLTPGGDITAYLPTNVMSITDGQWILDPKVFRDTMRPGVNIGLSVTRVGARGQSRR
                     QKQLAAQAVAALNAYKSAQEFAHFGSELALAAQKDLQTGKRLYELLSQGPDDTYSLSA
                     QTLMLDLALNAPEGRLVDMKRLKEAAVSLGGKVKDDKDYDKIKAEVLEKSTVELKK"
     gene            1134..1988
                     /locus_tag="VFH39_04040"
     CDS             1134..1988
                     /locus_tag="VFH39_04040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6924972.1"
                     /translation="MKRPQDIAVEEATMGTIVNLTSAFEGLASMRIASIKDQVQQSEQ
                     FFGELWQIYQQIRIDPTFRFGRENDHAPIDKELIIAITAEGGFSGDIDQKLIRWMIEQ
                     YDADKHDIIIIGHHGAVQLTEAGVAFKKYFKLPARDVSINVTPLMHEVQQYRDTTVFY
                     QTYVSLMVQDIKKIKLQTAVQEAGRQVAKDKDIISEKTYIFEPSTIAVIGHLERVMVG
                     IALSQTILESKLAQYASRFRAMSVARERAEDSKSELHLLFNRTRRATADERLKEITNG
                     LRHAGAVQ"
     gene            2134..>2926
                     /locus_tag="VFH39_04045"
     CDS             2134..>2926
                     /locus_tag="VFH39_04045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6924973.1"
                     /translation="MNQGVIRSLKELVVLVEFDDNSPVLNEVVRVRNERSTPLLVHAL
                     REGNIAVCLNLYGDRGLQKGMPVELTGQSIEIPVGDELIGRVVDALGRPIDGGQELGV
                     GLPRRNIFKVSARTTAFTAAKPEILETGIKVIDFFTPFVKGRKIGIIGGAGVGKTVLT
                     MELIHNIAKSGSGLSFFAGIGERIREGHELYETLAERDLLKNTCMFFGQMDQNPVQRS
                     LIGLSAVTLAEYFRDEQQKDILFFADNMYRYVQARNEVSSIMDQIP"
BASE COUNT          677 a          838 c          870 g          541 t
ORIGIN      
        1 actgataccg ctggtccgcg gccagcgtct ggccgtgctg ggcgacagca agaccggcaa
       61 aagtaccctg gccatgcagc tgacactcaa ccagaaggat agcgatgtgg tcgtggtgta
      121 tgtactgata gccaagcgta agagcgatgt cgaccagctg ctgtcgcgtc tgcaggagga
      181 ggacgcgctc aagagcgcta tcgtcgtggt agccaccatg ttcgaatcac tgataatgag
      241 ctacttggcg ccatacgtcg gctgtgccct cgccgaatat ctatggcaga agggcgacac
      301 cgatgtggtg gtagtgtacg atgatctcac cagtcatgct catgcttacc gcgaaatctc
      361 gctgctggcc ggtgtgagtc ccggccgtga tagctatccg ggcgacatgt tcttcgctca
      421 ctcgagcctg ctggaacggg cgggccggct gaaggccaac cacaagacgt tgaccgcgct
      481 gccgttggtg ctgacaccgg ggggcgacat caccgcctac ctgccgacta acgtcatgtc
      541 cataacggac gggcagtgga tactcgatcc caaagttttc cgtgatacca tgcgcccggg
      601 cgtgaacatc ggtctgagcg tcacccgcgt gggtgcccgc gggcagagca ggcgccagaa
      661 acagctggcc gctcaggctg tagcggcgct gaatgcctac aaatcggccc aggagttcgc
      721 tcatttcggc tccgaactgg cgctggccgc gcagaaagac ctccaaaccg gcaagcgcct
      781 gtacgagctg ctcagccagg ggcctgacga cacctacagc ctgtccgcgc agacgcttat
      841 gctggacctg gcgctcaacg cgcccgaagg ccggctggtc gacatgaagc ggctcaaaga
      901 ggccgccgtg agcttaggag gcaaagtcaa ggatgacaag gactacgaca agataaaggc
      961 cgaagtactg gaaaaatcga ccgtggagct taaaaaatga caatggcatc tagtaaccgg
     1021 gaagtagaaa ctagcgggct tattgcctca agcgggcaag acgtgtttgc gcccgctccc
     1081 tgtcggctgt ttcctggctc ctatttccta actcctgcga gcggagcgag cttgtgaagc
     1141 ggccgcagga catagccgta gaggaggcca ccatgggcac tatcgtcaat ctgacgagtg
     1201 ccttcgaggg tctggccagc atgcgcatcg cttccattaa ggaccaggtg caacagtcag
     1261 agcagttttt cggcgaactg tggcagattt accagcagat acgcattgat cctaccttcc
     1321 gcttcggccg cgagaacgat cacgctccga ttgataaaga actcatcatt gccatcactg
     1381 ccgagggcgg tttcagcggc gatatcgacc agaagcttat tcgctggatg atcgagcagt
     1441 acgatgccga caaacacgac atcatcatca tcggccacca cggcgccgtg cagcttaccg
     1501 aggccggcgt agcgttcaaa aagtatttca agctgccggc ccgcgacgta agcatcaacg
     1561 tgacgccgct gatgcatgag gtacagcagt accgcgacac gaccgtgttc taccagacgt
     1621 acgtatcgct gatggtgcag gatatcaaga agatcaagct gcagactgcc gtgcaggaag
     1681 ccggccggca ggttgctaaa gacaaggaca tcatcagtga gaagacctac atcttcgaac
     1741 cgagtaccat tgccgttatc gggcacctgg aacgagtgat ggtgggtata gcactcagtc
     1801 agaccatact ggagtccaaa ctggcccagt acgccagccg gtttcgcgcc atgagcgtcg
     1861 cccgcgaacg tgccgaggac agcaagagcg aactgcactt gctgtttaat cgtacccggc
     1921 gcgccaccgc ggacgaacgg ctcaaagaga taaccaacgg cctccggcat gcaggagcag
     1981 tgcaatgata aggtcaggaa tcaagaggca agaaccggga agcagaaacg gccggacggc
     2041 catggtgctt ggccgtcaat gctgccgcgg acggaccgcc gatagccgtt tcccggttcc
     2101 agcctctcat tacttacaga acggagctct gcgatgaatc aaggcgtcat acgttcgttg
     2161 aaggaactgg tggtgttggt ggagttcgac gacaactcgc cggtgttaaa cgaggtcgtg
     2221 cgcgtcagaa acgagcgcag cacaccgctg ctggtgcatg cgctgcgcga aggcaacatc
     2281 gccgtctgcc tgaacctcta cggtgaccgc ggattgcaga aaggcatgcc ggtagaactg
     2341 accgggcaaa gcatcgagat accggtaggc gacgaactca tcggccgcgt ggtagacgcc
     2401 ctgggccggc cgatagacgg cggccaggag ctcggcgttg gcttgccgcg gcgcaatatt
     2461 ttcaaggtat ccgcccggac caccgcgttt acggcggcca agcctgaaat actggaaacc
     2521 ggcataaagg tcatagattt cttcacgccg ttcgtcaaag gccgcaagat cggcatcatt
     2581 ggcggcgccg gcgtcggcaa aacggtattg actatggaac tcatacacaa tatcgccaaa
     2641 agcggcagcg gtctttcctt cttcgccggt atcggcgagc gcatccgcga gggccacgag
     2701 ctgtacgaga cactggcaga gcgcgacctg ctcaagaaca cctgcatgtt cttcggccaa
     2761 atggatcaga atccggtaca gcgctcgctt atcggtctca gtgccgtcac gctggccgag
     2821 tatttccgcg acgagcagca gaaggacatt ctgttcttcg ccgataatat gtatcgctat
     2881 gtccaggcca ggaacgaagt ctcgtccatc atggatcaga tcccgg
//
LOCUS       DASSFD010000165         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_189422, whole genome shotgun sequence.
ACCESSION   DASSFD010000165 DASSFD010000000
VERSION     DASSFD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189422"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1032)
                     /locus_tag="VFH39_04050"
     CDS             complement(94..1032)
                     /locus_tag="VFH39_04050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6924974.1"
                     /translation="MSKMIHTPGVVNVDDHSAVSATFAIEPLHTGYGMTLGNSLRRVL
                     LSSIAGAAVTAFKVEGATHEFTTVAGVKEDVVDIMLNLKAVRFRVFGDDAQTLRIVKK
                     GKGTVTAKDIQVNADVEVVNPEQVIATIDDDKAEFIMDLVVETGRGYRTIEEGAASKA
                     SDMIAVDAIFSPVLRVRYKVENTRVGQITDLDKLLITVDTDGSITPREAFEEAAAILV
                     NQYTALAGQTRIETKAAASSASDATTDDGDEPAELMTSIEDLNLSARTTNALINNDIH
                     TLKDLFSLSDSELRELKGFGSKALDEVKEKLAELEL"
     gene            complement(1053..1667)
                     /gene="rpsD"
                     /locus_tag="VFH39_04055"
     CDS             complement(1053..1667)
                     /gene="rpsD"
                     /locus_tag="VFH39_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET6924975.1"
                     /translation="MARDTQSIVKMSRREGYALHPKAHKALVKRSTVPGEQGRNQRNK
                     PSQYALQLREKQKVKRLYGLLERQFAKLMAEASRKQGQSGALLLQFLEQRADNAVYRA
                     GFAPSRRAARQLMTHGHFMLNGRRVDIPSIRLKAGDVLTLRDHSKNNEYFKKIDEVSP
                     APSDTPSWLKVNRKKFEVTVTGVPTREEAEPDINEQLIVEYYSR"
     gene            complement(1686..2081)
                     /gene="rpsK"
                     /locus_tag="VFH39_04060"
     CDS             complement(1686..2081)
                     /gene="rpsK"
                     /locus_tag="VFH39_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6924976.1"
                     /translation="MADSSTANKKKKAKRSVPAGQVHILATFNNTIISFTDPKGGVLA
                     AASAGACGFRGSKKGTAYAAQVATERAGQSVKQLHGFSKADVFIKGVGLGREAAVRTL
                     SSLDIQVESIKDVTGVPHGGVRPRKTRRV"
     gene            complement(2097..2483)
                     /gene="rpsM"
                     /locus_tag="VFH39_04065"
     CDS             complement(2097..2483)
                     /gene="rpsM"
                     /locus_tag="VFH39_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6924977.1"
                     /translation="MARISGVTIPDNKQVHVALTYVYGIGPKTSADILAAAKVEPTVR
                     AKDLTDAEIARIQDFINENYTVEGELQRVVATNIKRLKDINSYRGIRHKNNLPSRGQR
                     TRTNGRTRRGRRVAVGGSQPKAASKT"
     gene            complement(2487..2603)
                     /gene="rpmJ"
                     /locus_tag="VFH39_04070"
     CDS             complement(2487..2603)
                     /gene="rpmJ"
                     /locus_tag="VFH39_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET6924978.1"
                     /translation="MKVRASVKKISPDDKIVRRKGRLYVINKLKPKHKQRQG"
     gene            complement(2654..2875)
                     /gene="infA"
                     /locus_tag="VFH39_04075"
     CDS             complement(2654..2875)
                     /gene="infA"
                     /locus_tag="VFH39_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6924979.1"
                     /translation="MAKNKEVIELTGVVTEALPGTQFRVELENGHQIIAHIAGKMRKH
                     YIRLVPGDGVTVELTPYDLTKGRITYRKS"
     gene            complement(2936..>3432)
                     /locus_tag="VFH39_04080"
     CDS             complement(2936..>3432)
                     /locus_tag="VFH39_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HET6924980.1"
                     /translation="FQPYIYPVVYFLLVFIFTFFYTSITFNAKEIAENLQKQGGFIAD
                     IRSGSATEQYLSRVVNRLTLFGAIALGLIALLPIIGQIFLNTNIAIGGTSVLILVSVS
                     LETLRSIESRALMVTYDQYEQPDFLYDAAPVGLAGDTLAIKRPRILGVIPRRKTNAKT
                     TSKK"
BASE COUNT          728 a          887 c          967 g          850 t
ORIGIN      
        1 tgacgaggct gtcagcgagg cccttcatga gagcccggcg ctgatcgcgt tcccgatgga
       61 actttcttcc ttgatatccg tgacggtgca tgactagagc tccaactccg ctaacttttc
      121 tttaacttca tcgagagcct tgctaccaaa acctttgagt tcacgcagtt cagaatcgct
      181 cagtgagaac agatccttca gagtgtggat gtcgttgttg atcaaagcgt tggtggtgcg
      241 agctgacagg ttgagatctt cgatcgaagt catcagctct gcaggctcgt cgccgtcgtc
      301 ggtcgtcgca tcgctggcgc tgctggcagc agccttggtt tcgatacggg tctgaccggc
      361 gagtgcggtg tactggttga ccaggatagc agccgcttcc tcaaaggcct cacgaggcgt
      421 gattgagccg tcagtgtcga cagtaataag cagcttgtcg aggtcggtga tttggccgac
      481 acgggtgttt tcaaccttgt agcggacacg cagaaccggg ctgaagatag catcgacagc
      541 gatcatatcg ctggccttgc tggctgcgcc ttcttcgata gtgcggtagc cacggccggt
      601 ctcaacaacc aggtccatga tgaattcggc cttgtcatcg tcgatcgtgg cgataacttg
      661 ctcggggtta acaacttcta cgtctgcgtt aacctgaata tctttggcag taaccgtgcc
      721 cttgcccttc ttaacgatac gcagggtctg cgcatcatcg ccgaagacgc ggaagcgtac
      781 ggccttgagg ttgagcatga tgtcgacaac gtcttctttg acgccggcga cagtcgtgaa
      841 ctcatgggtt gcgccttcta ctttgaaagc agtaacggcg gcaccggcga tgctggaaag
      901 cagcacgcgg cggaggctat tgccaagggt cataccgtag ccagtgtgca gtggttcgat
      961 cgcgaaggtg gcgctgacag cgctgtgatc gtcgacgttg actacacctg gagtgtggat
     1021 catttttgac atatttcttc tatcgcctcc cgttagcgtg agtagtattc cacgattaat
     1081 tgctcattga tatctggttc tgcctcttcg cgagtaggaa caccggtaac cgtaacttca
     1141 aacttcttgc gattgacctt cagccagctg ggggtatctg agggagcggg gctgacttca
     1201 tcgatcttct tgaaatattc attgttcttg ctgtgatcac gcagtgtcag cacgtcaccg
     1261 gccttgaggc ggattgacgg gatatcgaca cggcggccgt tcagcatgaa atgaccatgg
     1321 gtcatcagct ggcgggcagc gcggcgtgac ggtgcgaaac cggcgcggta aaccgcgtta
     1381 tcagcacgct gttcgaggaa ctgcaggagc aatgcacctg actgaccctg cttgcgggaa
     1441 gcttcggcca tcagcttggc gaactggcgc tccagcagac catacagacg cttgaccttc
     1501 tgcttttcac gcagctgcag cgcgtactgg ctgggcttgt tgcgctggtt gcggccctgt
     1561 tcaccaggaa cggtcgagcg cttgactaat gctttgtgag ccttagggtg aagggcgtaa
     1621 ccttcgcggc ggctcatctt gacgatggat tgggtatctc ttgccatata taatctccga
     1681 caaccctaaa cgcgacgagt cttacgagga cggacgccac cgtgtggcac gcctgtaacg
     1741 tctttaatgc tttctacttg gatatcaagg ctggagagtg tccggacggc ggcttcgcgg
     1801 cccaggccta cacccttgat gaacacgtca gctttggaaa aaccgtgcag ctgcttgacg
     1861 gactggccgg cgcgctcggt tgcaacctgc gcggcgtaag ccgtaccctt cttggagcca
     1921 cggaaaccgc aggcgccggc gctggctgct gccaggacac cgcccttagg atcggtaaag
     1981 ctgatgatcg tgttgttaaa cgtagccaaa atgtgcacct gaccggcagg aacagaacgc
     2041 ttggcttttt tcttcttatt cgctgtagat gaatctgcca tttcttaaat cctatcctat
     2101 gtcttagatg ctgccttagg ctgtgaacca ccgactgcta cgcgacgacc ccgacgagtt
     2161 cgaccattgg tgcgggtacg ctggccccgg ctgggaaggt tgttcttgtg ccggatgcca
     2221 cggtagctgt tgatgtcttt gaggcgcttg atgttggtag cgacaacgcg ctgcaactca
     2281 ccttcaaccg tgtagttttc gttgataaag tcctgaatgc gagcaatctc ggcgtcggtc
     2341 agatccttgg cgcggactgt cggctcaact ttagcggccg ccaaaatgtc agcgctggtc
     2401 ttggggccta tgccgtagac ataggtcaat gcgacgtgaa cttgcttgtt atcaggaatg
     2461 gtaacaccgg aaatacgtgc catagattac ccctgacgct gcttgtgctt tggcttaagt
     2521 ttgttaataa cgtacaaacg ccccttacga cggacaatct tgtcgtcggg gctgatcttt
     2581 ttgacacttg cacgcacttt catgcggatc attgctcctc tctaatggtc aatgacacac
     2641 tgcttaaata gtattatgac ttgcgatagg tgattctgcc cttcgtaaga tcgtaagggg
     2701 tcaactcaac cgtcacgcca tcaccaggta cgaggcgaat gtagtgcttg cgcatcttac
     2761 cggcgatatg agctataatt tgatgaccgt tttcgagttc gacacgaaac tgagtacctg
     2821 gtaaggcctc ggtcactact cctgtcaact cgataacttc cttatttttg gccataaatg
     2881 tataacgtcg cttgattata ccagtgatta caacaacttg taaagagttt tcggatcact
     2941 ttttagaggt cgttttagcg ttagtcttgc ggcgtggaat gacgccgagt atccgcggcc
     3001 gtttgatagc cagcgtgtca cctgccagac cgaccggcgc ggcatcatac aggaagtcag
     3061 gctgctcata ttggtcgtag gtcaccatca gggcgcgcga ctcgatcgag cgcagcgtct
     3121 ccagggatac cgatacaagg atcaggacgc tagtgccacc gatcgcgatg ttggtgttca
     3181 ggaaaatctg gccgatgatt ggcagcagtg cgatcaggcc gagggcgatg gcgccgaaga
     3241 gcgtcagccg gttgacgacg cgtgacaggt actgctcagt cgcactaccg gaacggatgt
     3301 cggcgataaa gccaccctgc ttctgcaggt tctcggcaat ttccttggca ttgaaggtga
     3361 tactcgtata gaagaacgtg aaaatgaaga ccaacaggaa atagacgacc ggataaatgt
     3421 agggctggaa ac
//
LOCUS       DASSFD010000166         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_190434, whole genome shotgun sequence.
ACCESSION   DASSFD010000166 DASSFD010000000
VERSION     DASSFD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190434"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VFH39_04085"
     CDS             <1..1053
                     /locus_tag="VFH39_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6924981.1"
                     /translation="KMSKSKGNVVDPMVKIDQFGSDAFRMGIISDETPGSYRPYDESK
                     LVGARNFCNKLWNIARYIEDAVGEETDRAGVHPRSAADHWILSRLDRASQTVFAHLDS
                     YRFSEAYETLYHFVWDDVADWFVEASKAAPNKPLLAFILEAALTLAHPFAPFLTETIW
                     QTLAWEGLPAEASVLRSTTKDESAKAGDSLLATRTFASVPKYDSKHVKDFTEIQSIIG
                     EIRSIVNTLRVSGVTLYHTGVPFLNDNAELIKRLAKLQGVTEVKDGDGMYLTNTEYRC
                     WLDIDAGTARAYLKELDAQKTAQEAAIKRLESRLANKNYVKNAPRAVVAQTKDQLAAA
                     KGQLEKIWQEYKRFNQ"
     gene            complement(1380..>2005)
                     /locus_tag="VFH39_04090"
     CDS             complement(1380..>2005)
                     /locus_tag="VFH39_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6924982.1"
                     /translation="RTLLKTKSGVSFIYFDVNGCLVRFYHRAFARLAEETANPADVIE
                     TAFWHYNDAVCRGTMSMHDFNVALAGRLGVKRLDWQQFYLDAAEPVAEMHELLKWAAE
                     RYQVGLLTNIMPGFLKAMRERGQIPDLAYDAIVDSSEVGTIKPETRIYDIAQERAGCD
                     PGEILLVDDARTNLIAAEKNGWNVLWFDAYHTEESTANIRAALEPAS"
BASE COUNT          450 a          562 c          596 g          397 t
ORIGIN      
        1 aagatgagca agagcaaggg caatgtcgtt gatcctatgg tcaagataga tcagttcggg
       61 tcagacgctt tccgcatggg tatcataagc gacgagacac ccggcagcta ccggccgtac
      121 gatgaaagca agctggtggg cgcccgcaac ttctgtaaca agctgtggaa catcgcccgt
      181 tacatcgaag atgcggtcgg cgaggaaacg gacagggcag gggtccaccc ccgctcggcg
      241 gcagatcact ggatactcag ccggctggac cgggcgtcgc agactgtctt tgcccatctg
      301 gacagctacc gcttcagcga agcctacgaa acgctctacc acttcgtatg ggacgacgtg
      361 gccgactggt tcgtggaagc cagcaaggcc gcgccgaaca agccgctgct ggcattcata
      421 ctggaagctg ccttgacgtt ggcgcatccg ttcgccccgt tccttacgga aaccatctgg
      481 cagacgctgg cgtgggaagg cctgcccgcc gaagcttccg ttctccgtag tactacgaag
      541 gatgaatcgg cgaaggcagg ggacagcctg ctggcgaccc gcaccttcgc aagcgtgcct
      601 aaatacgatt ctaaacatgt taaggatttc accgagatac agagtattat tggtgaaatc
      661 aggagcatag ttaacacgtt aagagtttcc ggggtcacgc tgtaccatac gggcgtgcca
      721 ttcctgaatg ataacgctga acttattaag cgtttagcta aactgcaggg agtaacagag
      781 gtcaaagatg gcgacggtat gtacctcacg aacaccgaat accgctgctg gctggacata
      841 gacgccggaa ccgcccgcgc ctacctgaaa gaactggacg cccaaaagac cgcccaggag
      901 gccgccataa aacgactgga atcccgcctg gccaacaaaa actacgtcaa gaacgctccc
      961 cgcgccgtcg tggcgcagac caaagatcag ctggctgccg ccaaagggca gctggaaaaa
     1021 atctggcagg aatacaaacg attcaatcag tgagccggtg cttagttctt agaaatcgtt
     1081 cggcccctgg ccgaacctcc atggctgccc gggcccatca cggaggcctg actgcaggct
     1141 ggtgtccttg ggcgcgagga cgtgtctgac gacggagggt tgcctgtggc aagccgtagg
     1201 aggagttccg cagcgaacca gcctgcagtc aggctggagt gttctggggt cgtttctttg
     1261 cttactttct tgtcgataca agaaagtagg tgtgtccgac tacatcggac gcaaaaggat
     1321 gctcatgact ggatcccagc cttcgccgga atgacatgca atgaattcag gataataatt
     1381 tacgaagccg gttccagggc ggcgcggatg ttggccgtcg attcttcggt gtggtacgcg
     1441 tcgaaccaca atacgttcca gccgttcttc tcggcggcta tgaggttggt acgggcgtcg
     1501 tccaccagca gtatttcacc cgggtcgcag ccggcccgct cctgggcgat gtcgtagatt
     1561 ctggtctccg gcttgatggt accgacctcc gaggaatcga caatggcgtc gtaggcaagg
     1621 tcggggatct ggccgcgctc ccgcatggcc tttaaaaagc cgggcatgat gtttgtcagc
     1681 aggcctacct ggtagcgctc ggcagcccac ttgaggagct catgcatctc ggctaccggc
     1741 tcggcggcat ccaggtaaaa ctgctgccag tccaggcgct ttacccccag gcgccccgcc
     1801 aaggcaacgt tgaaatcgtg catgctcatg gtgccgcggc agactgcgtc gttgtagtgc
     1861 cagaaggcgg tttctatcac gtccgccggg ttggccgtct cttcggccag gcgggcaaat
     1921 gcccggtggt aaaaacgtac caggcagcca ttgacatcaa agtaaatgaa gctgacgccg
     1981 cttttggtct taagcagggt gcggc
//
LOCUS       DASSFD010000167         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_694156, whole genome shotgun sequence.
ACCESSION   DASSFD010000167 DASSFD010000000
VERSION     DASSFD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694156"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFH39_04095"
     CDS             <1..262
                     /locus_tag="VFH39_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924983.1"
                     /translation="LRFAFAFHQKRVNEAKNRQIISTIIEQLSGKKLHITCLYDPSLK
                     GQKVAAIAAAPTHPDAKTSAAATSGSLATISNIFGGGELLQS"
     gene            286..1647
                     /gene="dnaB"
                     /locus_tag="VFH39_04100"
     CDS             286..1647
                     /gene="dnaB"
                     /locus_tag="VFH39_04100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET6924984.1"
                     /translation="MPPRDKRATEAPQNTEAEASLLGAILIDSDAIVKVADSITAHDF
                     YDARHQHIYEAITTLYDKRSPIDVLTLSDQLKNAGYLDMVGGPAYLTELTNFVPTAAH
                     AEQYADIVAQKAMRRRLIAASQEMADLGYDEAKSLKELIEEAETKLFEVSQQHVKQDI
                     ISLEAILAESFERLDDLHKDKKKLRGVPTGYRDLDNMLAGLQRSDLFILAARPAMGKT
                     AFVLNLAHKVAVKAKEPVLIFSLEMGKEQLVDRLLAMESGVDAWALRTGNLTDNDFEK
                     IGEAMGTLSEAQIFIDDTPGITVSDLRTKARREAHQRPLGLIIVDYLQLMSGGSRFSS
                     SANRVQEISEISRGLKLVARELNVPLIALSQLSRSVESRVPPIPQLSDLRESGSIEQD
                     ADIVAFLYREDYYNPESERKNIMDVLIKKHRNGPTGGVELYFDKDKQRISSLETRHND
                     PFQ"
     gene            1693..>2189
                     /locus_tag="VFH39_04105"
     CDS             1693..>2189
                     /locus_tag="VFH39_04105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6924985.1"
                     /translation="MRRGSKKSGGKWPKWAAVAGCWLLVAGLLLYRLASLTHGPSQEE
                     FSISQQNGSLTAILRTPLFLPLHLLQWLIFTVTRHHGLYTLRVASVVFGLLAIAAFWY
                     VARKWYGLRTGLFATVLFAASAWTLHVSRYGGDSVMYLWALPALLLLLVLWEKPHKTW
                     LNTVVA"
BASE COUNT          518 a          606 c          621 g          444 t
ORIGIN      
        1 actgcgcttc gcgttcgcct tccatcagaa acgggtcaac gaggccaaga accgtcagat
       61 catcagcacg atcatcgagc agttgagcgg taagaagctg cacataacgt gcctgtatga
      121 cccgtcattg aaaggccaga aagtcgccgc cattgccgcc gcgccaacgc accctgatgc
      181 aaagacgtcc gcagccgcaa ccagcggttc tttggcgacc ataagcaata tatttggtgg
      241 cggtgagctg ttacaatcct aagtacgaag aagtgagaga ccataatgcc accgcgagat
      301 aagcgggcga ccgaagcccc acagaacacc gaagccgaag ccagtttgtt gggcgctatt
      361 ttgattgact cagatgccat cgtgaaggtg gcggacagta tcactgcaca cgacttctat
      421 gacgcccgtc accagcatat ttacgaggca atcacgacct tgtacgacaa gcgcagcccc
      481 atcgatgttc tcaccttgtc agaccagctc aagaacgccg ggtatctcga tatggttggc
      541 ggaccggcat atcttaccga gctgacgaac ttcgtgccga cggcagccca cgcggaacag
      601 tatgctgaca tcgttgcgca gaaagccatg cgacgccggc tgattgccgc ctcgcaggag
      661 atggccgacc tgggatacga tgaagcgaaa tcgctgaaag agctgataga ggaagccgag
      721 accaaactgt tcgaggtcag tcagcagcac gtcaaacagg acatcatcag cttggaagcc
      781 atcctggcgg aaagctttga acgtttggac gacttgcata aagacaagaa aaaactccgt
      841 ggtgtgccga ctggctaccg cgatctcgac aatatgctgg ccggcttgca gcggagcgat
      901 ttgttcattc tggcggcgcg tccagccatg ggtaagacgg ccttcgtctt gaacctcgca
      961 cataaggtgg ccgtgaaagc taaggaaccg gtgctcatat tcagtttaga gatgggcaag
     1021 gagcagctgg ttgaccggtt gctggctatg gaatcaggcg tggatgcctg ggcgctgcgt
     1081 accggtaatc tgacggacaa tgacttcgag aagatcggcg aggcaatggg taccttgagc
     1141 gaagcccaga tttttatcga cgacacgcct ggcatcacgg tcagcgatct acgcaccaag
     1201 gcgcgtcggg aggcgcacca gcgcccgctc ggcctcatca tcgtggacta tctacagttg
     1261 atgagtggtg gcagccggtt cagcagctcc gccaaccgcg tgcaagagat ctccgagata
     1321 tcgagagggc tcaaattggt cgcccgagaa ctgaacgtgc cgctgatcgc cttaagccag
     1381 ctaagccgtt ccgtcgaaag ccgggtgccg ccgattccgc aactgtctga cctccgtgaa
     1441 tcaggttcca tcgaacagga tgccgatatc gtggctttcc tgtaccgcga agattactac
     1501 aaccccgagt ctgagcgcaa aaacatcatg gacgtgctca ttaagaagca tcgtaacgga
     1561 ccgaccggcg gggtggagct gtacttcgat aaagataagc aacgcatcag ctcgctcgag
     1621 acacgtcata acgacccgtt tcagtaaaca ctggcggacc accccacgga cggctatact
     1681 aggtagcaac atatgagacg gggtagtaag aaaagcggtg gcaagtggcc aaagtgggcc
     1741 gcggtagcag gctgttggct gctcgtagcc ggtttactgt tgtatcgcct cgccagctta
     1801 acgcacggac ccagccaaga agaattcagc atcagtcagc agaacggctc actgacggcc
     1861 atattgcgca cgccgctgtt tttgccgctt cacttgctgc aatggcttat ctttaccgtc
     1921 acgcggcacc acggcctcta tactctccgg gtggcaagcg tagtattcgg tttgctggcg
     1981 attgctgcct tctggtatgt ggctcgcaag tggtacggtc ttcggaccgg cctcttcgcc
     2041 acagtattgt ttgccgcctc ggcctggacg ttgcatgtca gccgctacgg cggtgacagc
     2101 gtcatgtact tgtgggcatt gcctgcgctg ctcctactgc tggtgttatg ggaaaagcca
     2161 cacaagacat ggctcaacac ggttgtagc
//
LOCUS       DASSFD010000168         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_198812, whole genome shotgun sequence.
ACCESSION   DASSFD010000168 DASSFD010000000
VERSION     DASSFD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198812"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFH39_04110"
     CDS             <1..383
                     /locus_tag="VFH39_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924986.1"
                     /translation="TVMGKNGVPMNSSGYHPAIMVLCEENFQDTDPRRLKARLALYER
                     FYEDGVTDDNAPRLSQYILKTASFEMKRIQMMRRDRMTALRNIVTQQYIVPNLVEYRP
                     VGMSRQQRRAYERRQRRQGSRGKR"
     gene            710..2056
                     /locus_tag="VFH39_04115"
     CDS             710..2056
                     /locus_tag="VFH39_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HET6924987.1"
                     /translation="MISLIESVAPVLHEVARGGGGGSGAGGGGGGSIIAFIGYIPAHF
                     ITAWINRRVGKDPAIVAGAVVGVIVTAPCVVLNGGFGFIVGLGAVIGVYSGVNDWWGK
                     LARTFKKSRQQVESAALKDSAWDEAALEERVRQVFADYQQDWSDFNFAHMKKYLSPAY
                     YGFTEIFLRALQQMGRQNQVMNPAIQDLQFVAVQDEADNDADAFRVYLKAKADDRLID
                     TAAGTVIYEDTSSFRELWRFDREGNTWLLHDIQQVTADGTAKLAPLQQFAEQNGMFYS
                     LDMGWLLLPQHGQLFGKAKFKHSDVNNHVIGDWKGNIVMLYTYLPNPKNTGDTYVIGQ
                     LQLPKSYGGIIVKRRASGYGLDLFNIKPRGYNKVSFEWPDFNKRYNVYATDVDQVTSF
                     ELLNPSFMADLYDRNLPVNIEVVDNVVYLYAKVGGATTRYAEMLDVLQRAFKELRM"
     gene            2067..2492
                     /locus_tag="VFH39_04120"
     CDS             2067..2492
                     /locus_tag="VFH39_04120"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HET6924988.1"
                     /translation="MNIYYTDGSASPNPGPGGFAVILDKKPVVLGSEPGGGETTNIRM
                     EGFAIQAALRHADGQPCTIYTDSEFWINVITKWSLNWEKAGWKKKGGEIKNLDIVREV
                     CPLYRRSHATLVWVRGHNNDEGNELADHWANEARRRHLA"
     gene            2592..>2876
                     /locus_tag="VFH39_04125"
     CDS             2592..>2876
                     /locus_tag="VFH39_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6924989.1"
                     /translation="MLSECDMSPVLAVKDLQKGKEFYSQTLGLEVEKEDDDIILFSSG
                     DTMLQVYQSQYAGTNQATAATWEVEDLEDIVSELKDKGITFEQYDLPGASR"
BASE COUNT          694 a          750 c          879 g          553 t
ORIGIN      
        1 cgacggtaat ggggaagaac ggtgtgccga tgaacagcag tggctatcac cccgccatca
       61 tggtgctgtg cgaggagaac tttcaggata ccgaccctcg gcggcttaag gcccggttag
      121 ctctatatga acgtttttat gaggatggtg tgacggacga caacgcccct aggctttccc
      181 aatatattct taagaccgcc agttttgaga tgaagcgcat acagatgatg cgacgggatc
      241 ggatgacggc gctccgcaac atcgtgaccc agcagtatat agtgcctaac cttgtggagt
      301 atcggcctgt tggtatgagc cgccagcaac ggcgcgctta tgaacgacgc cagcgccgcc
      361 agggcagtcg cggcaagcgc taacgcttac gctgcggtgg ttgacaaaac cgttgcgctt
      421 atgctatttt cggggcagta agcgtaaatc cgcaaaaaga aatacactta caaaaacgct
      481 agaggagcaa accgaaaaaa taaggaatgt aaccaaaggc gaaaaagatc ctactagcta
      541 gaggagaaat aaaaagacag gtagcctgat cagcgcctgt ctttttattt tgacgacgct
      601 tcgctcgtgc ctacaaaagc taccgtcgat gccttttggg attgatcatc cggtattttt
      661 atttggcggc aaaggcgtga cggagcgcag cggtggtatc atggagtgaa tgatttctct
      721 tatagagtcc gtcgcaccgg ttctgcatga agtagcccgc ggcggtggag gcggctccgg
      781 cgcgggagga ggcggtggcg gcagtatcat cgcgtttatc ggctatatac cggcgcactt
      841 cataaccgcc tggatcaatc gtcgcgtcgg caaagatccg gccattgtgg ccggtgcagt
      901 ggtcggggtg atcgtgacgg cgccatgcgt ggttctgaac ggcggcttcg ggttcatcgt
      961 gggtctcggg gcggtgatcg gcgtctacag cggggtgaat gactggtggg gcaagctggc
     1021 acgcacgttc aaaaaatccc ggcaacaggt agagtccgcc gcgctcaaag attcggcctg
     1081 ggatgaggcg gcgctggaag agcgggtacg gcaagtcttt gcggactatc agcaggactg
     1141 gagcgacttc aacttcgcac atatgaagaa atatctgagc ccggcgtatt acggctttac
     1201 cgagatattc ctgcgggcgc tgcaacagat ggggcgccag aaccaggtga tgaacccagc
     1261 catccaagac ttgcagttcg tggcggtcca ggacgaggcc gacaacgatg ccgacgcctt
     1321 tcgggtgtac ttaaaggcca aggccgatga ccggctcatc gataccgccg ccggcacggt
     1381 tatatatgag gacaccagct cgttccgcga actgtggcgg tttgaccgcg agggtaacac
     1441 ctggctgctg cacgacatcc agcaggtgac ggccgacggc accgccaagc tggcgccttt
     1501 gcagcagttt gccgaacaga acggcatgtt ctatagcctg gatatgggct ggttgctgct
     1561 gccgcagcat ggccagctgt tcggcaaggc gaagttcaag cacagtgacg tcaacaacca
     1621 cgtcatcggg gactggaagg gcaacatcgt catgctgtat acctacctgc cgaaccccaa
     1681 aaacaccggc gatacctatg tgatcggcca gttgcagctg cccaagagtt atggcggcat
     1741 catcgtcaag cggcgggcga gcgggtacgg cctcgatctg ttcaatatta agccgcgggg
     1801 ctacaacaag gtgagcttcg aatggccgga cttcaacaag cgttacaatg tttatgctac
     1861 cgatgtcgat caagtcacca gcttcgagct gctgaacccg tcattcatgg ccgacttgta
     1921 cgaccggaac ctgccggtga acatcgaagt ggtagataac gtggtgtacc tgtacgccaa
     1981 agtcggcggc gccaccaccc gttacgccga gatgctcgat gtcttgcagc gggcgttcaa
     2041 agaactgcga atgtaaggag acaaacatga acatttatta caccgacggt agcgccagcc
     2101 cgaatccggg accgggcggc ttcgccgtca tcttagataa gaaacccgtg gtgctcggca
     2161 gcgaaccggg cggcggcgag acgaccaaca tccgcatgga aggcttcgcc attcaagcgg
     2221 cgctcaggca tgccgacggt cagccatgca ccatttatac tgacagcgag ttctggataa
     2281 acgtcatcac caaatggtcg cttaactggg aaaaggccgg ctggaaaaag aagggcggtg
     2341 aaatcaagaa tctcgacatc gtgcgcgaag tctgtccgct gtaccgccga agccacgcga
     2401 cactggtgtg ggtacgcggc cacaacaacg acgaaggcaa cgagctggcc gaccactggg
     2461 ccaacgaagc ccgccgccgg catctcgcat agggaggtga gcggtgtcac gagtgagttg
     2521 taggaattca ggttgccgcc tggtgagcgg gccgctcata ctctacagct gctaagacaa
     2581 agggggtgca tatgttaagc gagtgcgaca tgagcccggt gctggccgtg aaagacctgc
     2641 agaagggcaa ggaattctac agccagacgt tagggctgga ggtagaaaag gaagacgacg
     2701 atatcattct gttcagcagc ggcgacacca tgctgcaggt gtaccagagc cagtacgccg
     2761 ggaccaacca ggcaacggct gccacttggg aagtggagga ccttgaagac attgtcagcg
     2821 agctgaagga caaaggtatt acgttcgagc aatacgacct gcctggtgcc agtcgc
//
LOCUS       DASSFD010000169         5466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1218651, whole genome shotgun sequence.
ACCESSION   DASSFD010000169 DASSFD010000000
VERSION     DASSFD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218651"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFH39_04130"
     CDS             complement(<1..541)
                     /locus_tag="VFH39_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET6924990.1"
                     /translation="MAQQASFQAIGTLWDIQVDDDIDDEAWSQVMAQVHDRIDAFDGI
                     YSRFRPDSMVSRMAEQGGRHQLPPDGHKLLQFYERLYRATNGMVTPLIGQAMADAGYD
                     AAYSFRERPLQVSPAWEEVISYDEHVISLAEPALLDFGAAGKGYLVDIVAALLIMHGL
                     ASFTINAGGDVLHRSDTGIP"
     gene            complement(549..1085)
                     /locus_tag="VFH39_04135"
     CDS             complement(549..1085)
                     /locus_tag="VFH39_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924991.1"
                     /translation="MLNTYEQHSKRKLAATVLTIFVIAGTVLVADHMKSELSAAPKTF
                     TPAATSATTNNGAGTTQSSTATGSSGAGSATASTTSGYKDGTYTAASSYYVPHGNESI
                     QVNVTLSNGVITKSSVQNSEGDPTSASFQENFAASYKSYVVGQKLADLRLGVIAGASD
                     TTQGFANALSQIASQAQA"
     gene            1397..2467
                     /locus_tag="VFH39_04140"
     CDS             1397..2467
                     /locus_tag="VFH39_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924992.1"
                     /translation="MKQLQLAGDAYPSAVNRFATPRHIAMLGAAAISGPLAVDMASSY
                     VFWHGRHTEVIADTAEATVDQPRSLAVPGLANPTGEFLTTEFGDLLPGAKDYLSFSDY
                     GISAKKVGRALGAHYGKALGPNNTEGSSQNVICHSMGLLTFLMGAEYCKRKRLAVPPI
                     DTMIAVSPLLDASHTYFEKHIKFLARFPYPGGVLSKFCIELYQRCEAREQELSNLSTV
                     ALTALRNSHTGFSPILWGQQVRTGAYKPSSKYLEGVFTPNTKMYVVGDPEETTVKVLA
                     AVEACKAMVAPFGTSVTFVPAPGAGHSDMRIAADALRQHEDFSAKVGPTTAYDRIGAT
                     KGSIDPVTLSTVSAPEPMSTEA"
     gene            complement(2542..4326)
                     /locus_tag="VFH39_04145"
     CDS             complement(2542..4326)
                     /locus_tag="VFH39_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924993.1"
                     /translation="MFKLAKPSVAPDIFCLVLASVLLPTRLWLFSAVLFLYVISRRLP
                     RLTGSRAERFVWSYLLAFMGTIVLYCVAWVVRVKVSPGLLPVLLGVVAWLSLFVKASP
                     SKADRNAGSYGWISLVCAVLIMVFVSLPLLRHPDSAQVLRYAAKTGDDINHIGMIETD
                     RQMQGFFYGPKSASKNLIESGSINGYPQGWHMNGALFETGFLRLAGKDSAAGRLANYL
                     AYKTLWLGIAVFMVSELMLTFVRKLTTSKNRYEPLVLALAVLAIGGYLLIAVFGYGFQ
                     TFIAAGALLAAGLVAGMDYFAADTMPRHSLVLMAALGMASASVWLLTAPMLLIPAVIG
                     MVREAANRRKGSALASVGTLVLVLAFGVLSLLFLYVQIRYGKGTAGLDIGGATPPVWW
                     LAVLAIWLACWLLLWRQGRALLKPATAVFAIVFLEFALVALHQQLTLGTQRYYSIKLT
                     FLAAMMALTLLVPLIFHALWRERAAQRLAIGGLMLAVILLPFWTGLDIRKSLYPLKDS
                     ATISRPTAAAVLTNTSPNKRLIFLTDDPSESFLATKVSAALWVYGDIADRSAIANLSS
                     AVGSPAIWNTVQSGSSVGSGWHVIRPTQ"
     gene            4473..5420
                     /locus_tag="VFH39_04150"
     CDS             4473..5420
                     /locus_tag="VFH39_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924994.1"
                     /translation="MITPQLDTLPLKRQAQGLRQQLNRKQRLLKRSMLKSERTLLRWL
                     RSQYYPTAALRDCRQAVALNEDIMLAVVLIGAVICFSAVTILANIIFTFASAAYALAS
                     VSSFNLSVLTFAAIVVLIVLLTWIGALLQNSMAVALMEGMNHKQNRSLRVTLRRSLRF
                     TSRTAAAWLRVLSIAVTPFILWSFVIGIAMLTNLHAVTTPAFLIAFTVLCGIAVVWLL
                     GTYSLLPFVMLFEPAQSWIAGLRRSRQLVLTKGRLFIMSSYLLLAIALSAAYGLGLVI
                     QWLTPLASTPIFILLALGVLGMHNAVLTMLYRKRKLARK"
BASE COUNT         1274 a         1564 c         1453 g         1175 t
ORIGIN      
        1 gcggtatgcc agtgtccgag cggtgcagca cgtctccgcc ggcgttgatc gtgaacgaag
       61 caagcccatg cattatcaac agcgcggcga cgatatcgac caggtagcct ttgccggccg
      121 cgccgaagtc gagcaacgcc ggttcggcca acgaaataac gtgctcgtcg tagctgataa
      181 cctcttccca cgcaggcgac acctggagcg gccgctcacg gaacgaatac gccgcatcat
      241 agccggcgtc ggccatcgcc tggccgatca agggagtgac catcccgttt gtcgcccggt
      301 aaagccgttc gtagaattgc agaagcttat ggccatctgg gggcagctga tgccggccgc
      361 cttgctcagc catcctggac accattgaat cgggacggaa ccgggagtaa atgccgtcga
      421 aagcgtcgat gcggtcatgt acctgcgcca taacctgtga ccacgcttca tcatcgatgt
      481 catcatccac ctggatgtcc cacagcgtac cgatggcctg aaaactcgcc tgctgcgcca
      541 taagcaacct acgcctgtgc ctgggaagcg atctgactca aggcgttagc gaagccctgg
      601 gtcgtgtcgg acgccccggc gatgactccg agccgcaggt cggcaagctt ttgcccgacc
      661 acatagcttt tgtaagaggc ggcgaagttt tcctggaagc tggcggacgt cgggtcaccc
      721 tcgctgttct gcactgaact cttagttatg accccgttgc tcagcgtcac gttgacctga
      781 atgctttcat tgccatgggg gacataatag ctgctggcag cggtgtaggt accgtctttg
      841 tagccgctgg ttgtcgaagc agtcgcactg cccgcgcccg acgagccggt agcggtagac
      901 gattgcgtag tacccgctcc gttgtttgtc gtggcggagg tagcggcagg agtaaatgtc
      961 tttggagctg ccgatagctc agatttcata tggtcagcta ccagcaccgt accggctatg
     1021 acgaatatgg tcagaaccgt ggctgccagt ttgcgcttcg agtgttgttc gtatgtattg
     1081 agcatgtctt ctccgcttaa tttgcaaatc gtcaggaaca agatacgaga ccaactttaa
     1141 agtttcatta aaaatgcctg cttcacctat cccagagccc agccgtagac cagcgcagcg
     1201 catatttcga cacattactt atgtttgtga tgcacgaagg agtgctgcaa cagacgacac
     1261 ccaattccat acgggtaaag gcagcgcctc atacccgatg gctgctctac ggacctaaag
     1321 cccccaaagc cccggccgcg tagaaagcga actattacta agcttcggca ttagtggcgc
     1381 agtatactag aggcagatga aacaactcca gctagccgga gatgcatatc cttcggcggt
     1441 gaacagattt gccaccccgc ggcatattgc tatgctcggt gcggctgcta taagcggacc
     1501 gttggcagtc gatatggctt cgtcatatgt cttttggcat ggcaggcata ccgaagtcat
     1561 cgccgatacc gcagaagcta ctgtcgacca gccacgtagt ttggccgtac ctggactcgc
     1621 caatcccact ggtgagttcc ttaccaccga gttcggggat ctgctgccgg gagccaaaga
     1681 ttatctgtcg ttttccgatt atggcatttc ggctaaaaaa gtcggacgtg ccttaggcgc
     1741 ccattacggt aaagccctag gtccgaacaa tacggaagga tctagtcaaa atgtcatttg
     1801 ccacagtatg ggattgctca cttttctcat gggggcagaa tattgtaagc gcaagagact
     1861 agcggtaccg ccgatcgata cgatgatcgc tgtcagcccg ctgctggatg ccagccatac
     1921 ctacttcgaa aaacacatca aattcctggc tcgttttccg tatcccggcg gcgtactgag
     1981 caaattttgc atagaactat accagcgctg cgaggcccgc gagcaagagc tttcgaacct
     2041 cagcaccgtg gcactcactg ccctccgtaa ttcacataca gggttttcgc ctatactgtg
     2101 gggccaacag gtgaggactg gggcttataa accttcgagt aaatacctgg agggcgtttt
     2161 tacgcccaac acaaaaatgt acgtggtggg ggatcccgag gaaacgacag taaaagtact
     2221 ggcagccgtc gaagcctgca aggcaatggt agctccgttt ggcactagcg tgaccttcgt
     2281 tcctgcaccc ggcgccggcc actcagacat gagaatcgcg gccgatgccc ttcgtcagca
     2341 tgaagatttt tccgcaaagg ttggaccaac taccgcctac gacagaatag gcgccaccaa
     2401 aggcagcata gatccggtta cgctctcaac ggtttctgcc ccggagccaa tgagcacaga
     2461 ggcctgatcg aaccacgcac gtgagcggtt tcggtaacca atcccatagg cacttttact
     2521 taggcattga gactttgtat gttattgcgt gggccgaatg acatgccagc ctgagccgac
     2581 ggaagacccc gattggacag tgttccaaat ggccggagaa ccgacggctg aggaaaggtt
     2641 ggcgatagct gacctatcgg cgatatcgcc gtacacccac agcgcagctg acactttggt
     2701 agccaagaag ctttcgctcg gatcgtcagt caagaagata agacgtttgt tcggtgacgt
     2761 gttggtcagt acggccgcag ccgtcggacg gctgatggtg gcgctgtcct tcagcggata
     2821 gagtgatttg cggatgtcca ggccggtcca gaacggcagc aagattacag ccaacatgag
     2881 cccaccaatg gctaaacgtt gcgctgcccg ttcgcgccat aaagcatgga atatgagcgg
     2941 aacgagcagc gtcaaagcca tcatcgccgc caaaaaagta agctttatac tgtagtagcg
     3001 ttgcgtcccc agggtaagtt gctgatgaag cgccaccagc gcaaattcca ggaatacgat
     3061 tgcgaaaacc gcggtagccg gtttaagcaa tgccctgccc tgcctccaca acagcagcca
     3121 gcaagccagc catatagcca agacggccaa ccaccagacc ggtggtgtcg cgccaccgat
     3181 atcaagtccg gcggtgcctt taccgtatct gatctgcaca tacaaaaaca gtagcgacaa
     3241 gacgccgaac gccaggacga gcaccaacgt ccctaccgag gccagggcgc tgccctttcg
     3301 cctattggca gcctcccgga ccatcccgat cactgccggg atcagcagca tgggtgcggt
     3361 tagcaaccaa accgatgccg acgccattcc caacgccgcc attagtacca gactgtgcct
     3421 gggcatagta tcggctgcaa agtagtccat accggcgacc agcccggcag ccagtagcgc
     3481 cccggcagca atgaatgtct ggaagccgta accaaacacg gcaatcagta aatatccgcc
     3541 gatggccagt acagctagag ccagcaccaa aggctcatac ctgttttttg acgtagtcag
     3601 ctttcgaacg aatgtcagca ttagctcact gaccataaac actgcaatgc caagccacaa
     3661 tgttttgtag gccagatagt tggcgagccg gcctgccgct gagtccttac ccgccaggcg
     3721 caagaaccct gtctcgaaca gcgcaccgtt catatgccag ccttgcggat aaccgtttat
     3781 actgccggac tcaatgaggt tttttgatgc gctctttggt ccgtaaaaaa atccctgcat
     3841 ctgccggtca gtttcaatca tacctatatg gttgatgtca tcgccggttt tggcggcgta
     3901 acgcaatact tgcgcgctgt ctgggtgtcg tagcaaaggc aaggaaacga aaaccatgat
     3961 gagcactgcg caaaccaggc ttatccaacc gtatgaacca gcgtttcggt cagccttaga
     4021 aggcgacgct tttacgaata acgacagcca ggccaccacc ccgagcagta cgggtagaag
     4081 cccgggactc accttgaccc tgaccaccca ggccacgcaa tacagcacta tggtgcccat
     4141 gaaagccaac aagtagctcc atacgaatcg ctcagctcgc gaaccggtca gacgcgggag
     4201 gcgtcggctg atcacataca ggaataagac agcagaaaac agccaaaggc gcgtcggcaa
     4261 caagacggac gcaagcacca ggcaaaagat atcgggggcg acagagggct ttgcgagctt
     4321 aaacattgct tacgattata aaccactgct gaccgacatt aagcgtggct cactgctgtc
     4381 aaataatata attgcctttt aaactttttt atgctatcgt atgtatatta gtttttagtg
     4441 ggacgtcgca agcccggcga cgtaaaaagg tgatgataac tccgcaatta gacacgctgc
     4501 cgttgaaacg gcaagcccaa ggccttcgtc agcagctcaa ccgcaagcaa cggctgctga
     4561 agcgatcgat gctgaaaagc gaacggacac tgctccggtg gctgcgcagc caatattacc
     4621 cgacggccgc gctgcgtgac tgccgccagg cggtagctct taacgaagat atcatgctgg
     4681 cagtagtact gatcggcgcg gtcatctgct tcagcgccgt cactatcttg gcgaacatca
     4741 tatttacctt cgcttcagct gcctatgcgc tggcaagcgt atccagtttc aacctgagtg
     4801 tgctgacgtt cgcagccatc gttgtactga tagtactgct gacctggatc ggcgcgctgc
     4861 tacagaattc catggccgtc gcgctcatgg agggcatgaa tcacaagcag aaccgttcgc
     4921 tccgcgtcac gctccgccgc tcgttacgct tcacatcgcg gacagctgcc gcttggctgc
     4981 gcgtactcag tatcgcggtg acgcccttta ttctgtggag cttcgtcata ggtatcgcta
     5041 tgctgacgaa tctgcacgcg gtgaccacgc ccgccttcct cattgccttc accgttttgt
     5101 gcggtatagc cgttgtttgg ctgctcggca cctattcact gcttcccttc gttatgctgt
     5161 tcgaaccggc ccaatcctgg atagccgggc tgcggcgcag tcggcagctg gtacttacca
     5221 agggacggtt gttcatcatg agcagctacc tgttgctggc aatcgcgttg tcggccgctt
     5281 atgggctggg gctagtcatc cagtggctga ccccccttgc ttcgacgccg atcttcatac
     5341 tgctggcgct cggggtctta ggcatgcaca acgccgtcct gaccatgctg tatcgcaagc
     5401 gaaagcttgc ccgcaaatag ttctctatta cgctaaccgc acatatttca gcattgtgac
     5461 tattgt
//
LOCUS       DASSFD010000170         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1842413, whole genome shotgun sequence.
ACCESSION   DASSFD010000170 DASSFD010000000
VERSION     DASSFD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842413"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFH39_04155"
     CDS             <1..244
                     /locus_tag="VFH39_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924995.1"
                     /translation="VQAKELEQYAKATVGAVLATKEAKRLKAEKAAAEAFFNSPDSDV
                     SVDPNAVIAAEADAANHPINGPVAPADDKPKSRKSK"
     gene            256..1014
                     /locus_tag="VFH39_04160"
     CDS             256..1014
                     /locus_tag="VFH39_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924996.1"
                     /translation="MSSRRQYIDQAYLTSYLSGQSLNSDYSSVDDLISAAEEFIDAYV
                     GPQDKWFKASSNSNGVYTAPDEPIELPWVHEVRGRVATANSSTSFTLQQYQQNVFQMD
                     FFNLCSIEIIGGTGLGQTNHITGSTYQGLITVEKAWTTPLDTTSVYRIYQLGKFPRLR
                     DVYFDSINQPVRYYKTIPDGVRRAVAAQCVYMNTKGRDFFTSDAAMMTSEHLGSYSYT
                     RRLPKDASTRELFIAPEAKEALSGIVRRTGRIRT"
     gene            1017..1337
                     /locus_tag="VFH39_04165"
     CDS             1017..1337
                     /locus_tag="VFH39_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924997.1"
                     /translation="MAAMLRHRCDLARMATVGTNGRKAPSTLYTNVSCLFMPMSDRAA
                     VEGNYTVGQAWNVYFSPTADIKRGDKLTYNGTTYLVRGLKPYSGFGAMSYQQAAVETE
                     QANG"
     gene            1330..1812
                     /locus_tag="VFH39_04170"
     CDS             1330..1812
                     /locus_tag="VFH39_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924998.1"
                     /translation="MASSFVIEMHAEEAVARLGLLADEIPAHIRVWLVECAELAKKSI
                     EDRAPEGVAGAMGQGIKHNIDTVVTATEAWIGPNKNVPYAIYVEEGTRPHRPPAGPDS
                     ALAQWCEMKGLEPWAVATSIAKNGTQPHPFVEPAYEDIKDVIPAKFEEGIARMVGVDL
                     "
     gene            1809..2294
                     /locus_tag="VFH39_04175"
     CDS             1809..2294
                     /locus_tag="VFH39_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6924999.1"
                     /translation="MSYKGTSRKVKDCLVNILTNLTYDAGSGAEPLFVKVLDNTHDEF
                     EGYPIARVLPDRAASSVQDSISWLHEDSFAIIVTWPLTDPSNVESDLYNAAYDCADLV
                     RDTLEHDDHAATLNSIDPTLSWNFATVPSTSWRVATSKQGGAILALSLNVTISYSQTA
                     I"
     gene            2310..2462
                     /locus_tag="VFH39_04180"
     CDS             2310..2462
                     /locus_tag="VFH39_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925000.1"
                     /translation="MASTSDNQKPADTPKTSAPVAQDTFFVPSVGVVKAASAEAAATK
                     ANKQSK"
     gene            2478..3470
                     /locus_tag="VFH39_04185"
     CDS             2478..3470
                     /locus_tag="VFH39_04185"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HET6925001.1"
                     /translation="MANAPLLGRQESVGVGLESVSGTQAATLAWQPHLALTLDRKTNT
                     VENQSGMGRLEQTNDSAIVEQWAEGSLNGKIYWDTFGYFLSNIFGATPTASVHASETT
                     VYDNAFAIAQTSVPPCLSLVRANSIATRGFTMGMLSDLEIDIKQQDWIQFTATVQTKT
                     GATTTATAAYSTTEGDFTSKHATLSFAPYGSTSYAAAQTKSIKLKISRKLERFTPLGQ
                     TDPACFDIDSYTVTGTIVQRWTDTVLENIAAANTAQAMQLVIKNTDATIGTATNPSLT
                     ITCPKVRLDPATTDNKLDQTLSQTFNFEAQLDATGAGNLITAVLTNKKNGYTKS"
     gene            3451..3876
                     /locus_tag="VFH39_04190"
     CDS             3451..3876
                     /locus_tag="VFH39_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925002.1"
                     /translation="MATPSRKWSISKRVSFAELGDGWQDCYATVSELSYLERSEFVDR
                     RNDVLAGNDAKDSDLAAYVLSLVKAHFVDGKMLDDGQVVDMTVDDLDTAPGSVINLLL
                     DGTLGVVPDPKGSSTGTETSPTTTPTPELPTNTNSTKTS"
     gene            4103..>4702
                     /locus_tag="VFH39_04195"
     CDS             4103..>4702
                     /locus_tag="VFH39_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925003.1"
                     /translation="MAGFDSIIGITIKAIDGASGVVSAAAKNIEATQESVASTSAVSA
                     ARIGSAMDTAGEKMTALGSAMTRGVTLPLAAVAGASAKMAYDFQNQMELIRTSAGIPQ
                     DQIAGLSDKILALAGQVGQAPEKLAQGFYHIASAGNGIWNTAQMMDILKVAAQGANLG
                     LADMDTTTYALSSTMASGISGVKDASDAMAVLNAIVGAGD"
BASE COUNT         1089 a         1611 c         1300 g          702 t
ORIGIN      
        1 cgtgcaggcc aaggaactgg aacagtacgc caaggccacc gtcggtgccg tcctggcgac
       61 caaagaggcc aagcgcctga aggccgagaa ggccgccgcc gaggcgttct tcaacagccc
      121 cgacagcgac gtatcggttg accccaacgc cgtcatcgcc gccgaagcag atgctgccaa
      181 ccaccccatc aacggccccg tcgcacccgc tgacgacaag cctaagagcc gcaagagcaa
      241 ataaggcgat aaccagtgag tagccgccgc cagtatatcg accaggcata cctgaccagc
      301 tacctgagcg gccagagcct caacagcgac tacagctcag tcgatgacct gattagtgcc
      361 gccgaagagt tcatcgacgc ctacgtcggc ccacaggaca agtggttcaa ggcgtccagc
      421 aacagcaacg gcgtctacac cgcacccgat gagccgatag agctgccgtg ggtgcatgag
      481 gtacgcggcc gggtggcgac cgccaacagc agcaccagct tcacgctcca gcagtaccag
      541 cagaacgtct ttcagatgga cttcttcaac ctgtgcagca tcgagatcat cggcggcacg
      601 ggcctcggcc agaccaacca catcaccggc agcacttacc agggcctcat caccgtcgag
      661 aaagcctgga cgacgccgct ggacaccacc agcgtctacc gcatctacca gctcgggaag
      721 ttcccccgcc tgcgcgacgt ctacttcgac agcatcaacc agccggtgcg ctactacaag
      781 accatccccg acggcgtacg ccgggccgtg gccgcccagt gcgtctacat gaacaccaag
      841 ggccgcgact tcttcaccag cgacgccgcc atgatgacca gcgaacacct cggcagctac
      901 agctacaccc gtcgcctccc caaagacgcc tcgacccgtg agctgttcat cgcccccgag
      961 gccaaggaag ccctcagcgg catcgtccga aggactggtc gcatccgcac atgagcgtgg
     1021 ccgccatgct ccggcaccgc tgcgacctgg cccggatggc gacggtcggc accaacggcc
     1081 gcaaagcccc ttcgacgctg tataccaacg tctcctgcct gttcatgccg atgagcgacc
     1141 gggcggccgt cgagggcaac tacaccgtcg gccaagcctg gaacgtctac ttcagcccga
     1201 cggctgacat caagcgcggc gacaagctga cctacaacgg cacgacctat ctggtacgcg
     1261 gcctcaagcc ctacagcggc ttcggagcca tgagttacca gcaggccgcc gtcgagacgg
     1321 agcaagccaa tggctagttc cttcgttatc gagatgcacg ccgaagaggc ggttgcccgg
     1381 ctgggactgc ttgctgacga gataccggcc cacatccgcg tctggctggt ggagtgcgcc
     1441 gagctggcca agaagtccat cgaagaccgc gccccggaag gcgtggccgg cgcgatgggc
     1501 cagggcatca agcacaacat cgacaccgtg gtcacggcca ccgaagcctg gataggcccg
     1561 aacaagaacg tcccctacgc catctacgtc gaggaaggca cccgaccgca ccgaccaccg
     1621 gccggcccgg acagcgccct ggcccagtgg tgcgagatga aggggctgga gccgtgggcg
     1681 gtggctacca gcatcgccaa gaacggcacc cagccgcacc cgttcgtcga gccggcctac
     1741 gaggacatca aagacgtcat cccggccaag ttcgaggaag gcatcgctcg catggtgggg
     1801 gttgacctat gagctacaag ggaaccagcc gcaaggtcaa agactgcctg gtgaacatcc
     1861 tgaccaacct cacctacgac gccggcagcg gcgctgagcc gctgtttgtc aaagtgctcg
     1921 acaacaccca cgacgagttc gagggttacc cgatagcccg cgtcctgcct gaccgggccg
     1981 ccagcagcgt gcaggactca atcagctggc tgcacgagga cagcttcgcc atcatcgtca
     2041 cctggccgct gaccgacccc agcaacgtcg agagcgacct ctataacgcc gcctatgact
     2101 gcgccgactt ggtacgcgac accctcgaac atgacgacca cgccgccacc ctcaacagca
     2161 tcgacccgac gctgagctgg aatttcgcca ctgttcccag cacgtcatgg cgcgtggcta
     2221 cctctaaaca aggcggcgcg attctcgccc tgagcctaaa cgtaacaatt tcgtatagcc
     2281 agacggcaat ttagtgttat atttgagata tggcaagcac aagcgataac caaaagccag
     2341 ccgatactcc caaaaccagc gccccggtcg cacaggacac gttctttgtg ccttcggtcg
     2401 gcgtggtcaa ggccgcttcg gccgaggcag ccgccaccaa ggcgaacaag caaagtaaat
     2461 aaagggaata gaagtatatg gcaaatgcac cactactcgg ccgacaggag agcgtaggcg
     2521 tcggactcga aagcgtaagc ggcacacagg cggccacact ggcctggcag ccgcacctcg
     2581 cactgacgct cgaccgcaag accaacaccg tagagaacca gagcggcatg ggccggctgg
     2641 aacagaccaa cgacagcgcc atcgtcgagc agtgggccga agggtcgctg aacggcaaaa
     2701 tctactggga taccttcggg tacttcttat ccaacatctt cggtgcaaca cccacagctt
     2761 ccgtgcacgc cagcgagacg accgtctacg acaacgcctt cgctatcgcc cagacatcgg
     2821 tgccgccctg cctgtcactg gtacgggcca acagcatcgc cacccgtggc ttcaccatgg
     2881 gcatgctgag cgacctggaa atcgacatca agcagcagga ctggattcag ttcaccgcca
     2941 ccgtccagac caagaccggc gcgaccacca ccgccacggc cgcctacagc accactgagg
     3001 gcgacttcac ctcgaagcac gccaccctga gcttcgcccc ctacggctcg accagctacg
     3061 ccgccgccca gaccaagagc atcaagctga agatcagccg caagctggag cgcttcacgc
     3121 cgctcggcca gactgacccg gcctgcttcg acatcgacag ctacaccgtc accggcacca
     3181 tcgtgcagcg ctggacggac accgtgctgg agaacatcgc cgccgccaac accgcccagg
     3241 cgatgcagct ggtcatcaag aacaccgacg ccaccatcgg caccgccacc aacccgagcc
     3301 tgaccattac ctgccccaag gtgcgcctcg accctgccac caccgacaac aagctcgacc
     3361 agaccctcag ccagaccttc aacttcgagg ctcagctcga cgcaacaggc gcaggcaacc
     3421 tcattaccgc agtgctaacc aacaagaaga atggctacac caagtcgtaa gtggtctatc
     3481 tccaagcgcg tcagcttcgc cgagctaggc gatggctggc aggactgcta cgccaccgtc
     3541 agcgagctga gctaccttga gcgctcggag ttcgtcgacc gacgtaatga cgtgctggct
     3601 ggcaacgatg ccaaggacag cgacctcgcg gcctatgtgc tgagtctcgt caaagcgcac
     3661 ttcgtcgatg gcaagatgct cgatgacggc caggtggtcg acatgaccgt cgatgacctc
     3721 gacaccgcgc ccggttcggt catcaacctg ctgctcgacg gcaccctcgg agtggttcct
     3781 gacccaaaag gttcgtcgac cgggacggaa acgtcaccga ctacgacgcc tacgcccgag
     3841 ctgccaacga atacgaactc tacgaagacc tcttagttca cgagttcaaa ccggggccgg
     3901 agcacgccga agccgtcaag cgcatcgcgc atatccgcga aaagcaacgc ctcggcacgt
     3961 atgcccgtcg catgggcatg acgcttgaac aggtacggaa tgagtcagcc ggcgacatcg
     4021 agttcgcatt tcttatatgg tcactcgata gtgctcgtgc taatctaaaa gcaggtaaca
     4081 acagcgaata acaaaacaat acatggcagg cttcgacagc atcatcggta tcaccatcaa
     4141 ggccatagac ggagccagcg gcgtcgtctc ggccgctgcc aagaacatcg aagccaccca
     4201 ggaatccgtc gctagtacgt ctgccgtttc tgctgcccgt atcggctcgg cgatggatac
     4261 agccggcgaa aagatgacgg ctctcggctc tgctatgacc cgaggcgtca cgctgccact
     4321 ggcggccgta gctggagcga gcgccaagat ggcgtacgac ttccagaacc agatggagct
     4381 gatacgcacc tcggccggca tcccgcaaga ccaaatagcc ggattatcag acaagattct
     4441 cgccctcgcc ggccaagtcg gccaagcacc tgaaaagctc gcccagggct tctatcacat
     4501 cgccagcgcc ggtaacggta tctggaacac ggcgcagatg atggacattc tgaaggtcgc
     4561 cgcccaaggt gccaacctcg ggctggccga catggatacc accacttacg ccctgagttc
     4621 gacgatggcg agcggtatct ccggcgtcaa agacgcttcc gacgcgatgg ccgtactgaa
     4681 cgccattgtc ggtgccggcg ac
//
LOCUS       DASSFD010000171         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_632863, whole genome shotgun sequence.
ACCESSION   DASSFD010000171 DASSFD010000000
VERSION     DASSFD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632863"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VFH39_04200"
     CDS             complement(<1..1137)
                     /locus_tag="VFH39_04200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6925004.1"
                     /translation="MPIQTRRGYAYKSSRAPHSRPQRAPRQQRKGYGQYIDPAKFVKV
                     AHPVTPDEYIPTHEFMDFAVDSRLLANLSRKGFHLPTPIQDKTIPLGLEGKDVIGIAN
                     TGTGKTAAFAIPILHRLMTDPTAKALIVAPTRELAMQIEEECRTIGKGGGFSGALLIG
                     GSAYGPQLRDLRARPRIIIGTPGRIMDHLREGSLKLNDCNLVVLDEVDRMLDMGFVND
                     VRKLLGDLAASRQSFYFSATMDRRVAELIDGFSPDAATVSVRQGDTSENVHQDIIPYA
                     DKSSKLEGLHTLLIEPLVTKAIIFESTQRNVERLERELQNRGFEVTSIHGGKTQGQRK
                     RALDGFKASKPRFLIATDVAARGIDVPDVSHVINYGMPQTYEDYT"
     gene            1476..1862
                     /locus_tag="VFH39_04205"
     CDS             1476..1862
                     /locus_tag="VFH39_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET6925005.1"
                     /translation="MKQPDQRGFSIVETLLVLVIIGALSFVGYYVQHARKNADVSLDA
                     ATNSARSASTQSTSTEPGTTTSGHSNGSSGTPSGYGKTPVAVATNTSVTPNNNKDLQA
                     ALANSDPNDSQSQQSMNDASSALSTY"
     gene            1879..>2641
                     /locus_tag="VFH39_04210"
     CDS             1879..>2641
                     /locus_tag="VFH39_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925006.1"
                     /translation="MNLRTPLRTGAFTLALSTLLASSMLSIASAQTATPIVYPPMPPD
                     PTVLKMTILKGDQQISMRLNTIKKQTANVAGFKHVTGPLKTALTTQLSDAQNGNPKLK
                     LLGLVNLQKKLDKETSVTAAQADYNSIFTDYRIYMLVVPKIAIVNVAEMQQDRETNQN
                     KAIGILDTYVRAYNGSDKAVLELRLANDNSQLLKAVVTTQTVENVVLQLNAQDYNNDH
                     TVMKDNLTKLQANIKTLNTIRKDLKDLQNTLSQKKA"
BASE COUNT          616 a          735 c          738 g          552 t
ORIGIN      
        1 ggtgtagtct tcgtaggtct gtggcatgcc gtaattgatg acgtggctga cgtccggcac
       61 gtcgatgccg cgggcggcca catcggtggc gatgaggaac cgcggtttgc tggccttgaa
      121 gccgtcaagc gcccgtttgc gctggccttg ggtcttgccg ccgtggatgg aggtaacctc
      181 gaacccacgg ttctgcagct cgcgttccaa acgttcgacg ttgcgctggg ttgattcgaa
      241 gatgatcgcc ttggttacca gcggttcgat gagaagcgtg tgcagtcctt ccagcttgga
      301 cgacttatcc gcgtaaggaa tgatatcctg gtggacattt tcgctggtgt cgccctggcg
      361 caccgacacg gtggcggcgt caggtgaaaa gccgtcgatg agctcggcga cgcgccggtc
      421 catggtggcc gagaagtaga acgactgtcg tgacgctgca agatcgccga gcagtttacg
      481 gacgtcgttc acgaagccca tatccagcat gcggtcgact tcgtcgagca ctaccaggtt
      541 gcaatcattc agcttcagcg aaccttcgcg caggtgatcc atgatgcgtc ccggcgtgcc
      601 gatgatgatg cgcgggcggg cgcgcaggtc gcgcagttgc ggaccatagg cggaaccgcc
      661 gatgagcagc gcaccgctga agccgccgcc ctttcctata gtccggcact cttcctcgat
      721 ctgcatggcc agctcgcgcg tcggcgcgac gatgagcgct ttggcggtcg ggtcggtcat
      781 gagccggtgc aagatgggta tggcgaaggc tgcggtctta cctgtgccgg tattggcgat
      841 accgatgacg tccttgcctt ccagaccgag cgggatggtt ttgtcctgaa tgggtgtcgg
      901 caggtggaaa cctttgcggc ttaggttggc caacaggcgg ctgtcgacgg cgaaatccat
      961 gaactcatga gtcggtatgt attcatccgg cgtcaccggg tgagcgactt tgacgaattt
     1021 tgccgggtcg atgtattgac cgtagccctt acgctgctga cgcggagcgc gttggggacg
     1081 ggagtggggg gcacgactgc ttttgtaggc ataaccgcga cgtgtttgta tgggcataaa
     1141 aatagaatcc ttctgtagtt gttggttgtt tgctaggatt gcagctaccg aatcctaaaa
     1201 agcaaaaact acgaacaact tctggattcc tttggcgtcc gctggtgcgg acagctaaca
     1261 atgatgtaat tgtagtacga attacctcag aggtcaagag gaacgatcag gccggtctgc
     1321 gcataaagaa acgacagata catactttgc agctccgaag gcctgggaat cgttgcgtaa
     1381 aaaactgcgg aacggaaggt ttacggattg cgggcatata tccgctgcgc tatgatgccg
     1441 ccagaacgaa taacataaac aaggaataac agagaatgaa acaacccgat cagcgtggtt
     1501 tcagcattgt cgaaaccctg ttggtactgg tcatcatcgg tgcgcttagc ttcgtcggtt
     1561 attacgtgca gcacgcccgc aaaaatgccg acgtctcact ggatgccgcg acgaacagcg
     1621 cccgatccgc ttcgacccag tcaacatcga ctgagcctgg caccactacc agcggtcaca
     1681 gcaacgggtc ctccggtacg ccgtccggct acggcaaaac gccggtcgca gtcgccacca
     1741 atacgtccgt tacccctaac aataacaaag atctgcaggc ggctctggcc aacagcgacc
     1801 ccaacgacag ccaatcccag cagagtatga acgatgccag cagcgcgctg agcacctatt
     1861 aacggaaagg acataacgat gaatctacgc actcccctgc gcaccggcgc tttcaccctg
     1921 gctctcagta cattgttggc ctcttccatg ctttcaatcg cgtcagcgca aaccgccacc
     1981 ccgatcgttt acccgcccat gcctcctgac ccgacagtgc tcaagatgac cattttgaag
     2041 ggcgatcaac agatatcgat gcggttgaat accatcaaga agcagaccgc gaatgtagcc
     2101 ggtttcaagc atgtcaccgg accgctgaag acagctctta ccactcagtt gtcggatgcg
     2161 cagaacggca accccaaact aaagcttctc ggcctggtta atctgcagaa aaagctggat
     2221 aaagagactt ccgttaccgc cgcccaagcc gactacaact ctatctttac cgattaccgc
     2281 atttacatgc tggttgttcc caaaatcgct attgtgaacg ttgccgagat gcagcaggac
     2341 cgggaaacga atcagaataa agccatcggc atccttgata cgtatgtccg tgcctacaac
     2401 ggttccgaca aagccgtact tgagctgcgc ctcgctaacg acaactcgca gctgttgaag
     2461 gctgtggtta ccacgcagac cgtcgagaat gtcgtactgc agctgaacgc ccaggactac
     2521 aacaacgatc acacggtcat gaaggacaac ctgaccaagc tgcaagccaa catcaagacg
     2581 ctgaacacga ttcgcaaaga cctcaaagat ttgcaaaaca cgttgagcca gaagaaggcc
     2641 t
//
LOCUS       DASSFD010000172         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_58820, whole genome shotgun sequence.
ACCESSION   DASSFD010000172 DASSFD010000000
VERSION     DASSFD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58820"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFH39_04215"
     CDS             <1..366
                     /locus_tag="VFH39_04215"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925007.1"
                     /translation="KNGNLLTSPDIISRGFIYMREQEEIMNGLRNEVRRAVQQRFKRI
                     DLDRFKAELKDHITHFLFEQTKRSPIVIPVVNVIGGKTESAKTVHQVKSKSAEEIAAE
                     QQRRFQEMRARLLNQDQPD"
     gene            433..735
                     /locus_tag="VFH39_04220"
     CDS             433..735
                     /locus_tag="VFH39_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925008.1"
                     /translation="MANGPYPTPEEGGPQLSKAGPDATRDPQPVGELIKPLWINLSEA
                     EKAAAESRYNAASERVGHLVVSLAPPEEIKDALEELLAAKLGKFGILHSTTHRPNL"
     gene            759..>2079
                     /locus_tag="VFH39_04225"
     CDS             759..>2079
                     /locus_tag="VFH39_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET6925009.1"
                     /translation="MAKKKKSTKKKANTAPVAHEPSPFWGYAGAVLLMLVAICVVVGG
                     FGAGGPLPKALFQAAQWLFGYAGWILPVLLGVWGVNKFLGEERKVPRKKSWSLLATIV
                     LASAWLFTATATKQPDGSVTGGHGGQVGAVLGGSLLSLFDKVPASILFFVCTLAVGLY
                     TFGLWPSVIEMFRNMTARKENGDTDLAELKARAAATGFKLNEGVPVERHGAASAPEQP
                     APRLSSLRNTAQKLTVNEDHSALTAASDPNWQFPGVTLLDAKQDKADAGNVEGNARLI
                     KETFGNFNIDVEMEGANIGPRVTQYTLKPPTGVKLTKITALENNLALDLAATSIRMEA
                     PIPGKRAVGIEVPNIKPAIVRLSSILQSRDWTQAADPLTFAIGKDIAGKPVVANLAKM
                     PHLLVAGQTGSGKSVMINTFLTSLLYRNSPSDLKLILVDPKQVEMAPY"
BASE COUNT          503 a          610 c          586 g          380 t
ORIGIN      
        1 aagaacggca acctgctgac cagccccgac atcatcagcc gcgggttcat ctatatgcgt
       61 gagcaggagg agataatgaa cggcctgcgg aacgaagtgc gccgtgcggt tcagcagcgt
      121 ttcaagcgca tcgatctgga ccggttcaag gccgagctga aggaccacat cactcacttc
      181 ttgttcgagc agaccaagcg cagccctatc gtcataccgg tagtgaatgt catcggcggt
      241 aagacggaaa gcgccaagac ggtgcatcag gtaaagtcga aatcggcaga agaaatcgcc
      301 gccgaacagc agcgtcgctt tcaggaaatg cgtgcccgct tactcaacca ggatcaaccc
      361 gactagggta aaatccgaac aaaaagcgta aacgctcttg acaaatgtag taaaatttgc
      421 tatgcttcgt ttatggcaaa tggcccatac cccacaccag aagagggcgg tccacagcta
      481 agcaaagctg ggccggacgc aacgcgtgat ccgcagcctg taggcgagct gataaagccg
      541 ctctggatca acctcagcga ggctgaaaaa gcagctgccg agagtcgcta taacgcggct
      601 tccgagcggg tggggcactt ggtggtgtcg ctagcgccac cagaagaaat caaagatgcc
      661 ctggaagagc tgttggctgc taagctaggc aaattcggca tcctccattc gacaacccat
      721 agaccaaacc tctagtagca aggtataata aaacagttat ggcaaaaaag aaaaagagca
      781 ccaaaaagaa agctaacaca gcgcccgtag cgcacgaacc gtcacccttt tggggatatg
      841 ccggggcagt gctgctgatg ctggttgcca tctgtgtcgt cgtcggcggt ttcggtgccg
      901 gtggtccgct gcccaaggca ttgttccagg ccgcccagtg gttgttcggt tatgccggct
      961 ggatcctacc agttttgtta ggcgtatggg gcgtgaataa attcctgggc gaagagcgca
     1021 aagtaccacg caagaagtca tggagcctgc tggcgaccat tgtcctggcg tcagcctggc
     1081 tattcaccgc aaccgccacc aagcaaccgg acggctcggt taccggcgga cacggcggcc
     1141 aagtcggagc agtgctgggc ggttcgctgc tgtcattgtt cgacaaggtg ccggccagca
     1201 tactcttttt cgtctgtacc ctggctgtag ggctctacac cttcggtctg tggccatccg
     1261 ttatcgagat gttccggaac atgactgccc gtaaagagaa cggtgatact gatctggccg
     1321 agttgaaagc ccgggcggcc gccactggct tcaagctgaa cgaaggggtc cccgtagagc
     1381 gccatggtgc tgcctccgct cccgagcagc ctgcgccgcg gctgagctcg ctccgcaaca
     1441 ccgcgcagaa gcttacggtc aacgaagacc acagtgcgct caccgccgct agcgacccca
     1501 actggcagtt cccgggagtc actctgctcg atgccaagca agataaggcc gatgccggca
     1561 acgtggaggg caatgcccgc cttatcaaag agacgttcgg taacttcaat atcgacgtcg
     1621 agatggaagg cgccaacatc ggcccacgtg tcacccagta caccctcaaa ccgccgacgg
     1681 gcgtcaaact taccaagatc acggcactcg agaacaacct ggcgctcgac ctggctgcca
     1741 caagcatacg tatggaagcg cccatcccgg gcaagcgtgc cgtaggtata gaagtgccca
     1801 acataaagcc ggccatcgtc cggctcagca gcatcctgca gtctcgtgac tggacgcaag
     1861 ctgccgaccc gctgaccttc gccatcggta aagatatcgc cggcaaaccg gtagtggcga
     1921 acctggccaa gatgccccac ctgctggtag ccggtcagac cggttccggt aagtcggtca
     1981 tgatcaacac cttcttaacg agcctcctgt accgcaattc gccgtccgac ctgaagctga
     2041 tcctggtgga cccgaagcag gtcgaaatgg caccgtata
//
LOCUS       DASSFD010000173         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_956153, whole genome shotgun sequence.
ACCESSION   DASSFD010000173 DASSFD010000000
VERSION     DASSFD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956153"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1003)
                     /locus_tag="VFH39_04230"
     CDS             complement(182..1003)
                     /locus_tag="VFH39_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HET6925010.1"
                     /translation="MAETIEKIDWSELLDQALTREGKLSNVYNRFYEYSFLNQMLLAM
                     QGVYEPVATYKRWQDMDRQVVRGAQARSILRPIIIKDRAAKGETVVEDGQEPEGARLV
                     GFKLVRCLFPLSETTGKELPPAPEFPGWNRQTAEERLGISRIPFDISDGNVMGYSVGL
                     RYAINPLNPTPDRTAFHEYAHIVRGDTLPSLQDEYRQHRGRREVVAESIAYLCMHQLG
                     VLDDETALHSRGYIQDWRKGETLSDESVRDIFRTTDAILRAGRPAVPAEAQPDNS"
     gene            1164..1865
                     /locus_tag="VFH39_04235"
     CDS             1164..1865
                     /locus_tag="VFH39_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6925011.1"
                     /translation="MNRLDNVEVPSDKDINPDPRCACVLVLDTSASMGLPLQPGETLP
                     IDELNAGLRLFEEELRNDTLAARRVEVAIVTFGGGARMLMDFTAPDALQMPSLMANGD
                     TPMGTAITMAIDALERRKAYYRQQSLAVFRPWIFTLTDGQPNDDWQPVVPRIHQMDKD
                     RHFAFFAVGTDAADMETLAKIAPPWRPPRMLRGLAFKELFQWLSDSLGSSGVSGSRVE
                     DKNVPLKPTDGWAQV"
     gene            1862..2590
                     /locus_tag="VFH39_04240"
     CDS             1862..2590
                     /locus_tag="VFH39_04240"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6925012.1"
                     /translation="MSNWQIVSASVSGSHAEDALSDDALDIRTLADGTILIAVADGAS
                     SAAYSRQGAAWAVQSSLDWLQSQELTPEALPNTLGGALHCAHRFIADKGGNIEDYGCT
                     LLLAVITPACAAFSQVGDGFIIGDTDGMLRLLTQPFKGPYVNYTKFITDDGYDEAAST
                     ALVDTAQLRALMLMTDGLERVLVRQLTNMIFETPCRQLLDFAGNADVDDHNSELAAWL
                     SGGTVCSHNDDDKTLVLMVPRNSV"
     gene            2587..>2856
                     /locus_tag="VFH39_04245"
     CDS             2587..>2856
                     /locus_tag="VFH39_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925013.1"
                     /translation="MTVVYPRRIAQTVTLGQKVGGGGEGTVYKVENTILAAKVYSGPV
                     PLDKLRIMIAQPPAGVPTVSRPPNGHIRLAWPVDLIADRGRNGPVT"
BASE COUNT          598 a          834 c          840 g          584 t
ORIGIN      
        1 tagagcgagg tgcagcgctc ggcctcgacc gcctccatcg tggaggctgg ctcgaacgcc
       61 tccgccggga agacgatggt tgagccgtgc gtcatgcagg ccagcacgcc caggaccatg
      121 ccaaagcagt gctgctttgg aacacaaaag ttgccgcctg taagctggga taacgatgaa
      181 atcaggagtt gtctggctga gcttcggcag gtaccgctgg tcgtccagcc cgcaagatcg
      241 cgtcagtcgt cctgaaaata tcgcgcacag actcatcact cagcgtttcg cccttcctcc
      301 agtcttggat atatcctcgg ctgtgaaggg cggtttcgtc gtctaaaacg cctaactggt
      361 gcatacagag ataggcgatt gattctgcca caacttctct tctgcctcgg tgttggcgat
      421 actcgtcttg tagggaaggc aacgtatcac cacgcacgat atgcgcgtat tcatgaaatg
      481 ccgtgcggtc aggtgtcggg ttgagtgggt tgatcgcata cctgaggccg acagagtacc
      541 ccatgacgtt gccgtcagag atgtcaaacg gtatacgaga gatgcccagt ctttcctcgg
      601 cggtttgtct gttccatcct gggaactcgg gcgctggtgg caactctttg ccggtcgtct
      661 cagataatgg gaataaacat ctgaccaact tgaagccaac aagccgtgca ccctctggct
      721 cctggccgtc ttcgactact gtttcgcctt tagcggctcg gtctttgatg ataatcggtc
      781 tgagtattga tcgcgcctga gcgcccctga cgacttgtct atccatatcc tgccagcgct
      841 tgtacgtggc tacaggctca tagacacctt gcatagcgag caacatctga ttgaggaacg
      901 aatactcgta gaatcggttg taaacgttgc tgagcttacc ttcgcgggtc agagcctgat
      961 ctaacagctc gctccagtct atcttctcga tcgtttcggc caccaaacca gtgtagcatg
     1021 gcatatcccg acccaaagta cagcagtgct ggaacagccc aatggtaata cgctctgttg
     1081 gtacttctgt gtctagccca ccagccatac cgatgtgata ctcccagtag tagctgttct
     1141 ctaaccaagc aagtgaggtt gcgatgaatc ggcttgacaa tgtagaggtt ccgtctgaca
     1201 aggacattaa ccctgatccc cgctgtgcct gcgtactggt gctcgatacg tccgcatcga
     1261 tgggactgcc gctccaacca ggcgagacac taccgattga cgaactcaat gcggggctgc
     1321 gactgttcga ggaagaactc aggaacgata ccctagcggc ccgccgcgtc gaagtggcaa
     1381 ttgtaacttt cggcgggggc gcgcggatgc tcatggattt cactgccccc gacgccctgc
     1441 aaatgccgag cctcatggcc aacggtgata ccccgatggg tactgcaatt acgatggcca
     1501 tagacgcact ggagcgacgc aaggcatatt accggcagca gtcgctagcg gtattccggc
     1561 cctggatatt cacgctcacc gacggccagc cgaatgacga ctggcagccg gtcgtgccgc
     1621 gcatccacca aatggacaag gacaggcatt tcgcgttctt cgcggtgggt acagacgcgg
     1681 cagacatgga gacgctggcc aagatcgcgc cgccctggcg tccgccgcgc atgctgcggg
     1741 ggctggcgtt caaggagctg tttcagtggc tgtcggacag cctgggcagc agtggcgtgt
     1801 ccggtagccg tgtagaagac aagaacgtac cgctcaagcc gacagatggg tgggcgcagg
     1861 tatgagtaat tggcagatcg tgtcggccag cgtttcggga tcgcatgcag aagacgccct
     1921 aagcgacgat gccctcgaca tccgcacgct ggccgacggc accatcctca tcgccgttgc
     1981 cgatggcgca agcagcgcag catacagccg tcaaggcgcg gcctgggccg tccagtcttc
     2041 gctggactgg ctacagtctc aggaactcac gccggaggcg ctgccaaata cccttggagg
     2101 tgcgctgcac tgcgcgcaca ggttcattgc cgacaaaggc ggcaacatcg aagactacgg
     2161 ctgcacgttg ctgctggccg ttattacgcc tgcgtgcgcg gcgttttccc aagtcggcga
     2221 cgggtttatt atcggggaca cggacggtat gctccggctt ctgacgcagc cgttcaaggg
     2281 gccatacgtc aactatacga agttcattac cgacgacggc tacgacgaag cggcgtcaac
     2341 cgccctggtg gataccgccc agctccgcgc cctcatgctt atgaccgatg gcctggagcg
     2401 cgtactagtg cggcaactca ccaatatgat ctttgagacg ccctgccgcc agctcctaga
     2461 ttttgccggc aacgcggatg tcgatgatca caatagcgag ctggcagcgt ggctttccgg
     2521 cggcaccgtt tgcagtcaca acgacgatga caagacgctg gtgctcatgg tgccgagaaa
     2581 ctcagtatga ccgtagtgta tccgcggcgc atcgcgcaga cggtgacgct cggccagaaa
     2641 gttgggggcg gcggcgaggg caccgtgtac aaggtcgaga atacgatcct cgccgccaag
     2701 gtctacagcg ggccggtacc gctggacaag ctgcgtatca tgatcgccca gccgcccgcc
     2761 ggggtgccca cggtgagcag gccgccgaac ggacacatca ggctggcatg gcctgtcgac
     2821 ctgatagccg atagaggtag aaatggccca gttacc
//
LOCUS       DASSFD010000174         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_829664, whole genome shotgun sequence.
ACCESSION   DASSFD010000174 DASSFD010000000
VERSION     DASSFD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829664"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="VFH39_04250"
     CDS             <1..1810
                     /locus_tag="VFH39_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET6925014.1"
                     /translation="DSHHEAAGTGPLKPGTNVLIDTPDKLAGLNFGHAPELYVHSRCA
                     GKHGSDPQVLIVSSDGKTVYTLDLTKLDGEVLSSWLIHLGKTPLVGHDVKYTLKVLLE
                     LTGGARHPELVSGPRAVTADLPPVGHDTLMGAYLLNSLRREQSLTDLAQVDLGYDGSP
                     FEDMDAGEVMLRAPEIIAAIKALHAMQLKDLKAMPKVAGVAKDIEWPVIPVLARMEYI
                     GIELDTKYLAGFAEQIDDLVSDYEQQIYGYAEQEFNIGSPTQLAEILFEKLQLPKQGI
                     KKGKTGYSTAASELDKLRGSSPIIDLITQYREVAKLKNTYVDTLPKQVDENSRVHTTF
                     NLATAPTGRLSSNDPNLQNIPTRTDLGRHIRTAFVAGKGKKLVSADYSQFELRLAAYL
                     CGDQKLIDQFNGDVDVHVTTAAEIYGRNPEDVTKQMRRDAKVVNFGVMYGLSSHGLSI
                     ATGMTREAAQHFIDKYFEVRPKLLGYIESVKQKAAKEGYVETLLGRRRPTPDVNSSNF
                     AVREAALRAAVNMPFQGSAADIMKLAMIAADAKLRSQHNHCRLLLQIHDSVLVECPTE
                     IADRVAALLKETMENAYKLPVKLTVDTAIGDNWGEL"
     gene            1807..2412
                     /locus_tag="VFH39_04255"
     CDS             1807..2412
                     /locus_tag="VFH39_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6925015.1"
                     /translation="MSKNDETLAAYEEHVNEYVAGTPRTANGTVKDWIDRALSFVPVR
                     STVLELGSAFGRDADYMESLGYKVQRTDAAKGFVNLLRGQGKDARVLNAITDDLGGPH
                     AIVFANAVLLHFERTELQEVLQKVHAALAPGGVFAFTVKKGEGEEWSSAKLGAPRHFV
                     YWQAEQLQSYLEAAGFVDCAIEDGSTTNAEWLQVIAHKSAV"
     gene            complement(2401..2841)
                     /locus_tag="VFH39_04260"
     CDS             complement(2401..2841)
                     /locus_tag="VFH39_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925016.1"
                     /translation="MKLTYQTGVAALVQFIVMTILNFLNGIYGSVQECTSGGNDCLGN
                     VVLSLLFFLIVSTWFAFLAVLGYAAQDRRSRRLAQLLLATEALVALIALFDARHHPNI
                     LGLITSLADAALAIWVATLAYRLVRANGGRVTSARTRRRHRSDS"
     gene            2934..>3420
                     /locus_tag="VFH39_04265"
     CDS             2934..>3420
                     /locus_tag="VFH39_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6925017.1"
                     /translation="MKTKIVADHSILDVLNQEGGRTGAKLSKTDVHYRGLWHASSHIW
                     IYDGAGHVLLQKRTRMTWNYPGCWDISSAGHVDAGETGLAAARRELREELGVEARPSQ
                     LQLMKILRRAPWDPAYGQRHREYQYLYALQLPADTAFILEPLEVADVRWVHLEELMRA
                     AR"
BASE COUNT          808 a          972 c         1044 g          596 t
ORIGIN      
        1 ggacagccac cacgaagctg ccggtaccgg accgctcaag cccggtacga atgtactcat
       61 agacacgccg gataagctgg ccgggctgaa cttcgggcat gcccctgagc tgtacgtaca
      121 ctcgcgctgt gccggcaagc acggcagcga cccccaagtg ctcatcgtca gcagtgatgg
      181 caaaacggta tatacgctgg acctgaccaa gctggacggg gaggtcctca gttcctggct
      241 aatacatctc ggtaagacgc cgcttgtggg gcatgacgtg aagtacacgc ttaaagtgct
      301 gctggagctg acgggtggag cccgtcatcc tgaacttgtt tcagggcccc gtgcggttac
      361 tgcggattta ccgccggtgg ggcacgacac gctgatgggc gcctacttgc tcaacagctt
      421 aaggcgcgag caatcactaa ccgatctggc acaggtggac ctcggctacg atggctcgcc
      481 gttcgaagac atggatgccg gggaagtcat gctgcgggcg ccggaaatca ttgcggccat
      541 aaaagcgctg cacgcaatgc aactcaagga tctcaaggct atgcccaaag tggccggcgt
      601 agcgaaagac atagagtggc ctgttattcc ggtgctggcg cgtatggagt atatcggtat
      661 cgagctggac accaaatatc tggccgggtt tgccgagcag atcgacgacc tggtttccga
      721 ctacgagcag cagatatacg gctacgccga gcaggagttc aacatcggca gcccgaccca
      781 actggccgag attttgttcg aaaaactgca actgccgaag caggggatca agaagggcaa
      841 gaccggctac agcacggctg ccagcgagct ggacaagctg cgcggcagta gccccatcat
      901 cgacctcata acccagtacc gcgaagtcgc caagctcaag aacacctatg tggatacctt
      961 gccgaaacag gtggatgaaa actcgcgcgt gcacacgacg tttaatctgg ccactgcccc
     1021 gaccggcagg ctcagcagca acgatccgaa tctgcagaac atcccgaccc gcaccgacct
     1081 gggccggcac atccgcactg cgttcgtggc gggcaagggc aagaagctgg taagtgccga
     1141 ctacagccag ttcgagctgc gcctggcggc ttacctgtgc ggcgaccaga agctcataga
     1201 ccagttcaac ggcgacgtgg acgtgcatgt gaccacggcg gccgagattt acggccgcaa
     1261 ccccgaggac gtaaccaaac agatgcgccg tgacgccaag gttgtcaact tcggcgttat
     1321 gtacggcctg agctcgcacg gtctcagtat tgccaccggc atgacgcgcg aagcggccca
     1381 gcacttcata gacaaatatt tcgaggtccg ccccaagctg ctcggctaca tagaatcggt
     1441 caaacaaaag gccgccaaag aaggctatgt cgaaaccctg ctgggccggc gccggccgac
     1501 gcccgacgta aactcctcca acttcgcagt gcgcgaagcg gccctgcgcg cggccgtgaa
     1561 catgccgttc cagggcagtg cggccgacat aatgaaactc gctatgatag ccgcggacgc
     1621 caaactacgc tctcagcaca accactgccg tctgctgcta cagatccacg actcggtgct
     1681 ggtagagtgc ccaaccgaaa tagccgaccg agtggcggct ctgctcaaag aaaccatgga
     1741 aaacgcctac aaactgccgg taaaactaac cgtcgacacc gccataggcg acaactgggg
     1801 cgaactttga gtaagaatga tgagacgctc gcggcgtatg aagagcacgt taatgaatac
     1861 gttgccggta cgccacgaac ggcaaacggg actgttaaag attggattga ccgggctctg
     1921 agttttgtgc ccgttcgaag cacggtgctt gaacttggca gcgcgttcgg gcgggatgcc
     1981 gattatatgg aatcgttggg atataaggtg cagcgcacag acgccgcaaa aggctttgta
     2041 aatttgctgc gagggcaagg aaaagacgcc cgggtgctga acgcaatcac agatgatctg
     2101 ggcggtccgc atgcgatcgt tttcgcaaac gcagtcctgc tgcattttga gcgcaccgaa
     2161 ctgcaagaag ttttgcaaaa ggtgcacgct gcgcttgcac cgggcggagt attcgccttc
     2221 accgtgaaaa agggggaggg ggaggagtgg tcaagcgcca agcttggagc accccgccat
     2281 ttcgtgtact ggcaagcgga gcagctgcag tcatatttgg aagcggccgg gttcgtagac
     2341 tgtgccatag aggatggcag cactacgaat gcggagtggc tacaggtgat tgcgcataaa
     2401 tcagctgtct gagcggtgcc ggcgccgcgt gcgcgcagaa gtgacgcggc cgccattggc
     2461 acggaccagc cggtacgcga gggtggcaac ccagatggcc agggccgcgt cggccaggct
     2521 ggtgatcagg ccaagtatgt tcgggtggtg ccgggcgtca aacagggcta tcagggccac
     2581 tagcgcttcg gtggccagca gcagctgggc gaggcgcctg ctgcgtctgt cctgagcggc
     2641 atagcccagc accgccagga aggcgaacca ggtactgaca atcaggaaaa acagtagcga
     2701 cagcaccacg ttgcccaggc agtcattgcc gccgctggta cactcctgca cactgccata
     2761 gatgccgttc aaaaagttca atatggtcat gactatgaac tgcacgaggg cggccacacc
     2821 tgtctgatat gtaagcttca tgctactgat tatgccagaa acaaccgcaa acgaatacgc
     2881 gaaaaatcgt gcttgtattt gatcaagcat aagcgtatac cggaagtaga accatgaaaa
     2941 caaaaatagt tgccgaccac tccatactgg acgtgctgaa ccaggagggc gggcgcacgg
     3001 gcgccaaatt gtccaaaaca gatgtgcatt accggggttt atggcatgcc agctcgcata
     3061 tctggatata cgacggggcg gggcacgtgc tgctgcagaa acgcacgcgc atgacctgga
     3121 actatccggg ctgctgggat atcagcagcg ccgggcatgt tgatgccggc gaaaccggtc
     3181 ttgctgccgc ccgccgcgag ctgcgcgagg agctgggcgt ggaagcccgc ccaagccagc
     3241 tgcagctgat gaagatactg cggcgcgcgc cgtgggatcc ggcatacggc cagcgccacc
     3301 gcgaatacca atacctgtac gcgctgcaat tgccggcgga caccgctttt atactcgagc
     3361 cgctggaagt tgccgacgtg cgctgggtgc acctggaaga actgatgcgg gctgcgcggg
//
LOCUS       DASSFD010000175         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1158079, whole genome shotgun sequence.
ACCESSION   DASSFD010000175 DASSFD010000000
VERSION     DASSFD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158079"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFH39_04270"
     CDS             <1..550
                     /locus_tag="VFH39_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925018.1"
                     /translation="EPQVRQSLESDFPPEVIDGVTYEARGIMGASVNQALPIEEEVIS
                     DTARLVREADSIERSRAAGILDDEDATGIHPTVDAEDAVLTENELLLIAEPPYPIVEA
                     DHLDEVESEVILTAVRKATQLEKYRRVALDFLSRNAPSVVERKKSGVQLQIELDAPQK
                     NRSGGVLAGQVAVAIMVGLLRR"
     gene            563..1435
                     /gene="mutM"
                     /locus_tag="VFH39_04275"
     CDS             563..1435
                     /gene="mutM"
                     /locus_tag="VFH39_04275"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET6925019.1"
                     /translation="MPELPEVETVRAGLSRLIVGKRIKAVTHDTPKSFPNAEADVQQF
                     LIGAEIIAVKRRAKVLIVDLTGAYSLIIHLKMTGQLVFQGKGVHFGAGHPNDSLVHPL
                     PDKSTRVTFDFTDGSQLFFNDQRKFGWVRLLPTIEIPNLDFFKKVGPEPLSDDFTWQE
                     MRTRLLRRKGTNIKAALLDQSIIAGVGNIYADETLWGAKVHPTTLVRDLPDAKFKLLY
                     SALREVLQLSIDKGGSSSRDYVDAEGRKGSYLQFARVFRREGQACPRCGTTIIKTRVA
                     GRGTHICPHCQRVR"
     gene            complement(1432..>2210)
                     /gene="otsB"
                     /locus_tag="VFH39_04280"
     CDS             complement(1432..>2210)
                     /gene="otsB"
                     /locus_tag="VFH39_04280"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET6925020.1"
                     /translation="ASRTALLSENRIATLKADYANASSRLLLLDYDGVLMDFKKRFQD
                     ATPTPELSRLLKKLSNDEANTVVVISGRPRKDLQHWFEGNHIILVAEHGAFWRGPDGK
                     WHNVPVKVDKDWQDQILPLMYKYAANTPGAVVEEKEQALVWHYRNAKPYYAQKYLVGL
                     KRALTPVVRRLGLQMQQGNMILEVRPIGIDKGTATRRWLRHKPEFILALGDDYTDEDT
                     FNAMPDHAYTVKVGRGRTDARYRLKNVTATLKLLEELSKR"
BASE COUNT          443 a          647 c          665 g          455 t
ORIGIN      
        1 ggaaccgcag gtaaggcaat cgttggaatc tgatttcccc cctgaagtta tagatggtgt
       61 gacatacgaa gcacgaggca ttatgggcgc gagcgttaac caggcgctcc caattgagga
      121 agaggtcatt agcgacactg cccgtctcgt ccgcgaggca gatagcatag agcgctctcg
      181 ggcggcagga atattggacg acgaggatgc cacgggtatt catccaacag ttgacgcgga
      241 agacgcagtg cttaccgaga acgagctgtt gctgatcgcc gaaccgccat acccgatcgt
      301 cgaagctgac catttggatg aagttgagtc cgaagtaatt ctcacagctg tgcgcaaagc
      361 cacgcagcta gaaaagtatc gccgggtagc cctcgatttt ctgtcgcgca atgcgccaag
      421 cgttgtggag cgtaaaaaga gcggcgtgca gttacaaata gagctggatg ctccccagaa
      481 gaaccgatcg ggtggcgtgt tggccggcca agtcgcggta gccatcatgg tcgggttact
      541 gcgacgctga tacactggta ctatgccaga actaccggag gtcgagacgg ttcgggccgg
      601 cttgagccgg cttattgtcg gtaagcgcat caaggcggta acgcacgaca cgcccaagag
      661 ctttcccaat gccgaagcgg acgtgcagca gttcctgatc ggcgccgaaa tcatcgccgt
      721 caagcgccgt gctaaggtgc tcattgtgga cctcaccggc gcatattcgc tcatcatcca
      781 tcttaagatg accggccagc tagtgttcca gggcaaaggc gtgcacttcg gtgccggcca
      841 cccgaacgac agcctggtgc atccactgcc ggacaaatcc acgcgcgtta cgttcgactt
      901 caccgacggc tcgcagctgt tcttcaacga ccagcgtaaa ttcggctggg tgcgcctgct
      961 gccgaccatc gagatcccga acctcgattt cttcaagaaa gtcgggccgg agccgctcag
     1021 cgacgatttc acgtggcagg aaatgcggac gcgactgctg cggcgcaagg ggacgaacat
     1081 caaggcagcg ctgctggatc aatcgatcat cgccggtgtc ggtaacatct atgccgacga
     1141 gactttatgg ggtgccaaag tgcaccccac cacgctggtc cgtgacctgc cggacgccaa
     1201 gttcaaactt ctatattcgg ctttgcgcga ggtactgcag ctcagcatcg acaagggcgg
     1261 ctcgtcatca cgcgactacg tcgatgccga aggccgcaag ggcagttacc tgcagttcgc
     1321 gcgcgtcttc aggcgggaag gtcaggcctg cccgcgctgc ggcacgacca tcatcaaaac
     1381 ccgcgtcgcg ggccgcggca cccacatctg tccgcattgc cagagagtgc gctagcgctt
     1441 actcagttcc tcaagcagtt tgagggtcgc ggtgacgttc ttgagccggt agcgggcgtc
     1501 ggtccggccg cggccgacct tcacggtgta ggcgtggtcc ggcatggcgt taaaggtatc
     1561 ttcgtcggtg taatcatcgc ccagcgccag gatgaattcc ggtttgtggc ggagccagcg
     1621 cctggtagcc gtacctttgt cgatgccgat cggccggacc tccaggatca tattgccctg
     1681 ctgcatctgc aggcccagcc ggcgcaccac cggcgtcagg gcccgcttca gacccaccag
     1741 gtacttctgg gcgtagtagg gtttggcatt acggtaatgc cacacgagcg cctgttcttt
     1801 ctcttccacg accgccccgg gcgtattggc ggcgtacttg tacatgagcg gcagtatctg
     1861 atcctgccag tccttgtcta ctttcacggg gacgttgtgc cacttgccat ccggcccgcg
     1921 ccagaatgcc ccgtgctcgg ccaccagtat gatgtgattg ccttcgaacc agtgctgcag
     1981 atccttgcgc ggccgtccgc tgataaccac gaccgtgttg gcttcatcgt tcgataattt
     2041 cttgagcagg cggctcagct cgggggtggg agtcgcgtcc tggaagcgct ttttgaagtc
     2101 catcagcacg ccgtcgtagt cgagcagtaa caagcggctg ctggcgttgg cataatcggc
     2161 tttgagtgta gcgatacggt tttccgatag gagcgctgta cggcttgccc
//
LOCUS       DASSFD010000176         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1221916, whole genome shotgun sequence.
ACCESSION   DASSFD010000176 DASSFD010000000
VERSION     DASSFD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221916"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VFH39_04285"
     CDS             complement(<1..1213)
                     /locus_tag="VFH39_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET6925021.1"
                     /translation="MHIYFSGIGGTGIGPLALVAKQAGYTVSGSDKQDSQYIEYLKKH
                     GITDIHIGQTYEAIATEHAKNPIDWFVYTSALPMENPDAPELLFCREQDIHTSKRDEL
                     LNKIITDKHLKLIAVAGTHGKSTTTAMVIWLFKQLGVPIGYNVGAKMSFGDMGEFAPG
                     AEYFVYECDEFDRNFLAFEPYLSLISGVSWDHHDIYPTREEYQAAFRDFINQSRHTIL
                     WHEDAEYLVLDEEDGALAVELSDNPTINGIALKGRYTRLDAWLAVQAVHRVTGAPVED
                     LLHHVDRFPGLQRRMEPLVPNLYSDYAHTVDKIRGAMSVAREMADESGQDLVVVYEGL
                     HNKRQYYIMNDYKDCFDGAAQVYWVPSYLAREDPDQRILPPQELIMRLSDPSIAVPAE
                     LDGDLKRTIQAH"
     gene            1344..1949
                     /locus_tag="VFH39_04290"
     CDS             1344..1949
                     /locus_tag="VFH39_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925022.1"
                     /translation="MNKRRLHHYWTKFRGLKPWYFLAVGLVSGVICLLALRANNQHMV
                     QLRDAVYAADKSGQGVDTALNNLRRYVYGHMNTQLASGPDAVYPPIQLKYTYDRLVKA
                     ASDRTAANNSQIYTDAQHYCEQQDPVDFSGHNRVPCVMQYVSAHGIKTTVIPDSLYKF
                     DFVSPAWSPDLAGWSLVVTLVSLLLFAILFVVNRWFKQNVA"
     gene            complement(2166..2825)
                     /locus_tag="VFH39_04295"
     CDS             complement(2166..2825)
                     /locus_tag="VFH39_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925023.1"
                     /translation="MNRTEQAQSAPASNSGSTQASRSAASRRPEKEDNKWKRVGVVSM
                     LFALTVLVVALIVLVFVNPSNTQSKYIDSSKYQAVFLTNGQVYFGNLNSVGSDYFRLD
                     NIYYLTQNTTTNSNGQTTTDGNYTLVKLGCQQIHDPLDQMIIERNQVSFWENLNDSGK
                     VVTSIKQFQKQNPNGPDCSQVSTQTQSSNNATTNNQGGSSAATGTNSGTSTPSNSVKK
                     P"
     gene            3055..3252
                     /locus_tag="VFH39_04300"
     CDS             3055..3252
                     /locus_tag="VFH39_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925024.1"
                     /translation="MAYKHTNSKGVTYYLNSKEVTLRGGKKQKIYYFSKDERPEGTDL
                     PSDMSVNENPRNGFLTVKRNK"
BASE COUNT          701 a          880 c          963 g          759 t
ORIGIN      
        1 gatgggcctg gatggtccgc ttgaggtcgc catccagctc tgcgggcacg gcgatggacg
       61 ggtcgctgag gcgcatgatg agttcttggg gcggcaaaat gcgctggtcc gggtcttcgc
      121 gggccagata gctcggcacc cagtacacct gcgcggcgcc atcgaagcag tccttgtaat
      181 cattcatgat gtaatactgg cgcttgttgt gcagaccttc atagacgacc accaggtcct
      241 ggccgctctc gtcggccatc tcacgggcga cgctcatggc gccacggatt ttgtcgacgg
      301 tgtgggcata gtcgctgtac aggttgggaa caagcggctc catgcgacgt tgcaggccgg
      361 ggaagcggtc gacgtggtga agtagatctt ccaccggcgc gcccgtcacc cggtggacgg
      421 cctggacggc caaccaggca tcgagccggg tgtagcgacc cttcagggcg atgccgttga
      481 ttgtcggatt atcactcaat tcaacagcga gggcgccatc ttcttcgtct aagaccagat
      541 actcggcatc ttcgtgccac agtatggtgt ggcggctctg gttgataaag tcccggaagg
      601 cggcctgata ctcttcacgg gtcgggtaga tgtcgtggtg atcccagctg acaccgctga
      661 taaggctcag gtatggttca aaagccaaga agttgcggtc gaattcgtcg cactcatata
      721 cgaaatactc agctcccggg gcgaactcac ccatgtcacc gaagctcatc ttggcgccta
      781 cgttatagcc gatcgggacg ccaagctgct tgaacagcca gatgaccatg gcggtggtgg
      841 tgcttttgcc atgcgtgccg gcgacagcaa tcagtttcaa atgtttatcg gtaatgattt
      901 tgttgagtag ttcgtcgcgt ttgctggtat ggatgtcctg ctcacgacag aacagcagtt
      961 ccggcgcatc ggggttctcc atgggcagcg ccgaggtgta cacgaaccaa tcaatcgggt
     1021 tcttggcgtg ctcggtggcg atcgcttcgt aggtctgacc gatgtgaatg tccgttatgc
     1081 cgtgtttttt aaggtactcg atgtactgcg agtcctgttt gtcggagccg gacacggtgt
     1141 aaccggcctg cttggcgacc agcgccaaag ggccgattcc ggtaccgccg atgccagaaa
     1201 agtaaatatg catggttacc agtatatagc ttcgggcatc gtcacgctaa aggagtcgag
     1261 ctatgtggga aatagtaatg cgctaaagct gcgcaccgaa tcacgccgcg gtttccaaga
     1321 ggtaggctat aatcgtaacc agcatgaaca aacgacggct gcatcactac tggacgaagt
     1381 tccgcggcct taaaccctgg tactttttgg cggtgggctt ggtgagcggt gtcatctgcc
     1441 tgctcgcgct gcgggccaac aaccagcaca tggtgcagct gcgcgatgcc gtctatgccg
     1501 ccgacaaatc gggccagggt gtcgatactg ccctgaacaa cctgcgcagg tacgtctacg
     1561 ggcacatgaa cacccagctg gcgagtggtc cggatgctgt ttatccgcct atccagctca
     1621 aatacaccta cgaccgcctg gtgaaggcgg cgagcgaccg cacggcagcg aacaacagcc
     1681 agatatatac cgacgcccag cactactgcg agcagcagga tccggtcgac ttctcgggcc
     1741 acaaccgtgt gccgtgcgtc atgcagtacg tcagcgcgca cggtatcaag acaaccgtca
     1801 ttcccgactc gctctataag ttcgacttcg tctcaccggc ctggtcacca gatctggcag
     1861 gctggagcct ggtcgtgacg ttggtaagcc tattgctgtt cgctatcctg ttcgtcgtga
     1921 accgctggtt caaacagaac gtcgcctaaa ttacctaaag ccgataccca ctaacggtac
     1981 gttacagccg taccgtacct gaccacaggt aaagagtggt gtaacgcaac gaaactcttc
     2041 cgttgccagg tagtcgctat ctaacggtta gcacgggccg ggtgattact cctacgggtg
     2101 ggggcttatg cataagaaaa gacccggggt tggcccgggt ctttttgttc agcgaaccgt
     2161 cgaaactaag gcttcttgac gctgttgctt ggagtgctgg taccgctgtt ggttccggtc
     2221 gcagcgctgc taccgccttg gttgttggtg gtggcgttgt tgctggactg ggtctgcgtg
     2281 ctcacctggc tacagtcagg accgttcggg ttctgcttct ggaactgctt gatgctggtc
     2341 acgactttac cgctgtcgtt caggttctcc cagaaactga cctggttgcg ctcaatgatc
     2401 atctggtcga gcgggtcgtg aatctgctga caacccagct taacgagcgt gtagttaccg
     2461 tcggtcgtag tctgaccgtt ggagttggtc gtagtgttct gggtcaggta gtagatgttg
     2521 tccagacgga agtagtcgct gccgacggag ttcaggttac cgaagtacac ctggccgttc
     2581 gtcaggaaga ctgcctggta cttgctggag tcaatgtatt tgctctgcgt gttgctcggg
     2641 ttaacgaaga ccagtacgat caacgcaacg accaaaacgg tcaatgcgaa cagcatgctg
     2701 acgacgccga cgcgcttcca cttgttatct tctttttcgg ggcggcgact ggctgcgcta
     2761 cggctagcct gggtgctacc actgtttgat gcaggcgctg attgggcctg ttcggtacga
     2821 ttcatgaagt ccatcgtatc ttttaactcc acctttaaag tttttatggt taccgcctta
     2881 gtgtaataca gcccatcggc gactgcaact gtttataagc gtagctgtgg acagaacggc
     2941 gctccagatg ggcggctgcg ctcggtcgta tctggagcta cagggttgac aaataaaaac
     3001 ggccgggagt atgttagggg tactattaag tgttaagaga gggagaccat accaatggct
     3061 tacaagcaca ctaactcaaa gggtgtcaca tactacctga actcaaaaga agtgacgctc
     3121 cgcggcggca agaagcaaaa gatctactac ttcagcaagg acgagcgccc ggaaggtact
     3181 gacctcccta gcgacatgag tgtcaacgag aacccgcgca acggtttcct gaccgtcaag
     3241 cgcaacaagt aatcacgaat agtaaacaca ttggacaccc gccaaaaccg gcgggtgttt
     3301 tct
//
LOCUS       DASSFD010000177         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_595066, whole genome shotgun sequence.
ACCESSION   DASSFD010000177 DASSFD010000000
VERSION     DASSFD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595066"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFH39_04305"
     CDS             complement(<1..576)
                     /locus_tag="VFH39_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6925025.1"
                     /translation="MAVNLQIIGIAGTNGSGKDSTGLTLADRHSYLFVSVTDLLRAEL
                     NRRGVPVDRENLRKLSAEWRREYGLAVLVDRAIEEYNRLKGRYVGVAIASLRNPYEAD
                     RIHELGGIVLWVDADPKVRYQRIQNNAEARARAGEDSKTFEEFLAEEQTEMHAVNGDA
                     ASLDMSAVKKKADLFLVNDNTNLQDFQNFITE"
     gene            complement(647..1780)
                     /gene="rpoD"
                     /locus_tag="VFH39_04310"
     CDS             complement(647..1780)
                     /gene="rpoD"
                     /locus_tag="VFH39_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET6925026.1"
                     /translation="MAKATNKAKNNDAPAPSNDELKQQLFDKAKADGHIAQRDIFAVI
                     ADTPEHAELLDALYTELADANIEVTITEPNAAEFDDDWSEEESEEIAVEEAVYLDDDV
                     ADDSVRLYLREIGKIPLLNSEEELALAKRVVAGDEKAKHEMAEANMRLVVSIAKRYVG
                     RGLDLLDLIQEGNTGLLRAVEKFDPDKGFKFSTYATWWIRQAITRAIADQARTIRIPV
                     HMVETINKLLRTQRRLTQELNREPTNEEIAKAMEIEVDKVEHIMKIKQDISSLDASIR
                     DDDEDSVLADFIEDEDTVSPEESATGQLLKEQVKDMLGALTEREQKILKLRFGLEDGK
                     SHTLEEVGQEFSVTRERIRQIEAKALAKLRKHRDAKKLHDYIK"
     gene            complement(1831..3585)
                     /gene="dnaG"
                     /locus_tag="VFH39_04315"
     CDS             complement(1831..3585)
                     /gene="dnaG"
                     /locus_tag="VFH39_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6925027.1"
                     /translation="MDAVDEVKSRLAIEDVVGEYVQLKRAGRNWKGLSPFTSERTPSF
                     VVSPEKQIWHDFSSGRGGNVFSFVMEMEGLDFKGALELLARKAGVDLEQYPTAGSRRG
                     PNKERLFELLEATTHFYQVQFSKNKMALEYVFGKRKFSKETALAWRLGYAPNTGDALV
                     KYLGGKGFTPAEMKAAGVTAQRYRGTGDMFRGRLMIPLCDPQGRVIGFTARLLADDPQ
                     APKYINTPQTVLYDKSRHVFGLHHAKEAIRKSQFVVIAEGNLDVIASWQANVRQVVAT
                     AGTALTEYQLKALGRFTGDIRLSFDADRAGMQATERAIPIASRVKVSLSVIEIPSGKD
                     PDELIKQDPAIWQRIIQEPKYALDWLMERYQKLLDVTSAQGKRQYSDVLLPVVRALND
                     PVEQDHYLVAIAETIGVSREALNSKFARGASESARPLKRAKTEPAKLDKAQVEYSKAQ
                     DHFLGLMLLHPPLRDNLRVITGDMFSKDESKQLLKFLQAHPDFDGKPETAAELKPFAE
                     YAKILVLQYEELYRGLELTELQYEAARLQTRLVEQFVKTKKQNLAAHMQNADESTTQQ
                     LLSEARQLDTLLSQVKRG"
     gene            complement(3659..4129)
                     /locus_tag="VFH39_04320"
     CDS             complement(3659..4129)
                     /locus_tag="VFH39_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925028.1"
                     /translation="MPTPNQLQAPEFTQTPHATHGEIANWLQNEAVGNSPFAATYLAH
                     LRDMHEKFKDSANAELSEAVIGLTAVIAARSEGRLKDVATLNGGSLPVEIPGVEPLHT
                     VHDEDIYAAAADVMPDDPSSESYEGSFAMAQAHNAIAEATAAELQQMPHGPEVS"
     gene            complement(4166..4585)
                     /locus_tag="VFH39_04325"
     CDS             complement(4166..4585)
                     /locus_tag="VFH39_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HET6925029.1"
                     /translation="MKATLIFVVQRHDASHLHFDFRLQLGGVLKSWAVPKEPPTRPGI
                     KRLAIETTDHPTGYADFQGTIPKGEYGAGTVETWDKGTLEYILEGIETGSWAYDQGKL
                     EFTLHGNRLHGTYILTRIQGKGPKSHWLLIKQIDKKA"
     gene            4660..5409
                     /locus_tag="VFH39_04330"
     CDS             4660..5409
                     /locus_tag="VFH39_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925030.1"
                     /translation="MNPQVPVGPVPPNTPNPYQQPPSGQAEQPQPPYQPGPQAPIQSQ
                     LPQQQYGNPEHQQYDFIMNRGQAAARSFALPGSNSKLGRIALVAGIGVVLIILIAVVM
                     SFLNRPTISSVELVNAAQEQSELIRIAGIAANDQSAAQSTRNSAQTIQLSLGSAQSDL
                     LGYLALNHIKIGKDVLQQRKDAQTDTELTSAQTAGTFDSTYAQIMQSQLTAYQQTLKA
                     AYAAHPGPRGQKLLSDDYQAAGLLLVQLQQQ"
     gene            complement(5413..5736)
                     /locus_tag="VFH39_04335"
     CDS             complement(5413..5736)
                     /locus_tag="VFH39_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET6925031.1"
                     /translation="MDHKTVEAYILSMPDSRLEYPFGKDVAVYKTGDKMFALIAEGKD
                     PVSLSLKCDPALSKVLRERYESVMPGYHLNKKHWNTLVLTGQLSWPEVQDLIRHSYDL
                     VVSER"
     gene            5879..>6141
                     /locus_tag="VFH39_04340"
     CDS             5879..>6141
                     /locus_tag="VFH39_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925032.1"
                     /translation="MTRKIKNLVVNSSLSAMLAISSLGVALSIMLPQTAHAVAPTVVY
                     NALPSTSPAANYPSQPFQAQQTKEFGDAIHLGGTNRLLNTVTVT"
BASE COUNT         1133 a         1880 c         1688 g         1440 t
ORIGIN      
        1 ctcggttata aagttctgaa agtcctgcag gttggtgttg tcgttcacca ggaacaggtc
       61 ggccttcttt tttacggcgg acatgtcgag cgaagcggca tcaccgttga cggcatgcat
      121 ttcggtctgt tcttcggcca gaaattcctc gaacgtcttg ctgtcttcgc cggcgcgggc
      181 ccgggcctcg gcgttgttct ggatgcgctg atagcgtact ttcgggtcag catccaccca
      241 tagcacgatg ccgcccagtt catggatgcg gtcggcttcg tacgggttgc gcaaagaggc
      301 gatggctacg ccgacgtagc ggcccttcaa ccggttgtat tcttcgattg cccggtcgac
      361 gagtaccgcc aggccatact cgcgccgcca ttcggcgctg agcttgcgca ggttctcgcg
      421 gtccaccggc acgccccggc ggttcagctc ggcacgcagc aggtccgtga ccgacacgaa
      481 cagatagctg tgccggtcgg ccagtgtcag ccccgtggaa tctttgccgg agccgttcgt
      541 cccagcgata ccgataatct gcaaattaac tgccatgtcc ctccagttta catgatttgt
      601 atctgttaat cgagcgcccg caggcgcccg ataacaactt cctgtgctac ttgatgtagt
      661 cgtgcagctt cttggcgtcg cggtgtttgc gcagcttggc cagcgctttg gcctcgatct
      721 gacggatacg ctcgcgggtc acagagaatt cctgccccac ttcttccaag gtgtgcgatt
      781 tgccgtcttc caggccgaag cgcagcttca gaatcttctg ctcgcgctca gtcagggcgc
      841 ccagcatgtc cttgacctgc tccttcagca gctgaccggt ggccgattct tcagggctga
      901 ccgtgtcttc gtcttcgatg aagtcggcca gcacgctgtc ctcatcgtca tcgcggatgg
      961 aggcgtccag gctggaaatg tcctgcttga tcttcatgat gtgctctacc ttgtcgacct
     1021 ctatctccat ggctttggcg atttcctcgt tggtcggctc gcggttgagc tcctgggtca
     1081 ggcggcgctg ggtgcgcagc agcttgttga tggtctcgac catgtgcacc ggaatacgga
     1141 tggtgcgggc ctggtcggcg atggcgcggg tgatggcctg gcggatccac caggtggcat
     1201 aggtactgaa cttgaatccc ttgtccgggt cgaatttttc aacggcgcgc aacagaccgg
     1261 tgttgccttc ctgaatcaga tcgagcaggt ccaggccgcg acccacgtaa cgcttggcga
     1321 tactgacgac cagccgcatg ttggcctcgg ccatctcgtg tttagccttc tcgtcgccgg
     1381 ccacgacgcg cttggccaac gccagctctt cttcgctgtt gagcagcgga atcttaccga
     1441 tctcccgcaa gtacagccgg accgagtcat cggctacgtc gtcgtccaag tacacggcct
     1501 cttcgacggc gatttcctcg ctctcttcct cgctccagtc gtcgtcgaac tcggcagcgt
     1561 tcggctcggt aatcgtcacc tcgatattgg catcggccag ctcggtgtac agcgcatcga
     1621 gtaattcggc gtgctcgggc gtatcggcga taaccgcgaa gatgtcgcgc tgcgcgatgt
     1681 gcccgtcggc cttcgccttg tcgaacagct gctgcttcag ctcatcgttg cttggtgccg
     1741 gggcgtcgtt attcttggct ttgttggttg ctttcgccat gcatgctcct tcgtattatc
     1801 acttatacca gggggcgtgc cgcaggcaca ctatccccgt tttacctgac tgagcagcgt
     1861 atcgagttgc cgggcctcac tcagcaactg ctgcgtcgtg gactcatcgg cgttttgcat
     1921 gtgcgctgcc agattctgct ttttcgtttt tacaaactgt tccaccagcc gggtctgcag
     1981 tcgagccgct tcgtactgaa gttcggttag ctcgagaccg cggtagagct cttcatactg
     2041 taataccaat attttagcat attctgcaaa tggcttcaat tctgcggcgg tctcgggctt
     2101 gccgtcgaag tcggggtgag cctgtaggaa tttcagcagc tgcttcgact cgtccttgct
     2161 gaacatgtcc ccggttatga cccgcaggtt gtcgcgtagc ggcgggtgca acagcatcag
     2221 gcccaggaag tggtcctgcg ctttgctgta ctcgacctgc gccttatcga gtttggccgg
     2281 ttcggtcttg gcccgcttga gcggccgagc cgactcactg gcaccccgag caaatttact
     2341 gttgagcgcc tcgcgactga caccgatggt ctcggcgatc gccaccaggt agtggtcctg
     2401 ctccaccggg tcattcagcg cccgcaccac cggcagcagc acatcgctgt actggcgctt
     2461 gccttgcgcg ctcgtcacgt cgagtaattt ctggtaccgt tccatcagcc agtccagggc
     2521 gtacttcggc tcctgaataa ttctttgcca gatagccggg tcctgtttga taagctcgtc
     2581 cgggtccttg ccgctcggaa tttcgatgac gctgagcgac acttttaccc ggctggcaat
     2641 cggaatcgcc cgctccgtcg cctgcatgcc ggcccggtcg gcatcgaagc ttaagcggat
     2701 gtcgcccgtg aaccgtccaa gtgccttcag ctggtactcg gtgagcgccg tgccggccgt
     2761 ggccaccacc tggcgcacat tcgcctgcca gctggcgatc acatccagat tgccttcggc
     2821 gatgacgacg aactggcttt tgcggatggc ttccttggcg tggtgcagcc cgaacacgtg
     2881 tcggctcttg tcgtacagca cggtctgcgg cgtgtttatg tacttcggcg cctgtgggtc
     2941 gtccgccagc agtcgggccg taaagcctat gacacgcccc tgtggatcgc acagcggtat
     3001 catcagccgg ccgcggaaca tatcgcccgt accgcggtaa cgctgcgccg tcacaccggc
     3061 cgccttcatc tcggccggcg taaagccttt gccacccagg tatttgacca acgcatcacc
     3121 ggtattcggt gcgtagccga gccgccaggc cagcgccgtc tctttgctga acttccgttt
     3181 gccgaacacg tactccagtg ccatcttatt cttcgaaaac tgcacctggt aaaagtgcgt
     3241 cgtcgcttcc agcagctcaa acagccgttc cttattcggt ccgcggcggc taccggctgt
     3301 ggggtactgc tccagatcca ccccggcctt acgcgccagc agctccagcg cccccttgaa
     3361 gtccaagcct tccatctcca tgacgaagct gaacacattg ccgccccgcc cgctggaaaa
     3421 gtcgtgccag atctgcttct ccgggcttac cacgaagctg ggcgtccgct cgctggtgaa
     3481 cgggctcaac cccttccagt tgcgcccggc gcgcttgagc tggacgtact cgcccaccac
     3541 gtcctcgata gccaaccggc tctttacctc atccactgca tccatatctg ttacatattg
     3601 tacgctaaat aaaagagccg gtgtcaaacc ggcccttctg ccttatgaag ttatggagtt
     3661 agctaacttc tggtccatgg ggcatctgct ggagctccgc cgcggtagct tcggcgatcg
     3721 cattgtgcgc ctgcgccata gcaaagctac cttcatagct ctcgctgctc ggatcatcag
     3781 gcatcacatc tgcagctgca gcataaatgt cttcgtcatg aacggtatgc agtggttcga
     3841 cacccgggat ttccaccggt aggctgccgc cgttaagtgt tgccacatct ttcaacctac
     3901 cctcgctacg cgctgctatc acagcggtta aacctatgac ggcttcggac agctcagcgt
     3961 tggcgctatc cttaaacttt tcgtgcatat cacgtaagtg agcgagataa gtagcagcga
     4021 acggggagtt ccccacagct tcattctgta gccaattcgc aatttctcca tgcgttgcgt
     4081 ggggagtttg tgtaaattct ggggcttgca gttgattggg tgtgggcatt tttgttttcc
     4141 ttatactgcc tacaatgtat cacacttatg cttttttgtc aatttgctta attaataacc
     4201 agtggctttt tgggccttta ccttggatgc gggtgaggat gtaggtgccg tgcagccgat
     4261 tgccatgcag cgtaaactcc agcttccctt ggtcataggc ccagctgccg gtttcaatcc
     4321 cctccaagat gtattccagt gtgcctttgt cccaggtttc aaccgttccg gcgccatact
     4381 cgcccttggg gatggtacct tggaaatcgg cgtaccctgt ggggtgatcc gtcgtttcga
     4441 tggccaagcg cttaatgcca ggccgtgtgg gcggctcttt cggtacggcc cagcttttca
     4501 gcacgccccc taactgtaat cggaaatcga aatgcagatg gctggcatcg tgccgttgca
     4561 ctacgaaaat aagcgttgcc ttcataccgc cattgtaatg cctgtgcgag aacagttcag
     4621 tagttgaaca tgtacggaat ctgttaagat aaacataaga tgaatccgca agtcccggtt
     4681 gggcccgtgc cgccgaacac gccgaatccc taccagcagc cgccctccgg gcaggctgag
     4741 caaccgcaac ctccttatca gccagggcca caggcgccga tccagtccca gctgccgcaa
     4801 cagcagtacg gcaacccgga acaccagcag tacgacttca tcatgaatcg cggccaggct
     4861 gccgcacgca gcttcgcttt gcccggcagc aactcgaagc tgggccgtat cgctctggtc
     4921 gccggtatag gggtagtgct gatcattttg atagcggtcg tcatgtcttt cctcaaccgt
     4981 cccaccatca gcagcgtcga gctggtaaac gccgcccagg agcagagcga gctgatccgg
     5041 attgccggca tcgccgccaa cgaccagagt gccgctcaaa gcacgcgcaa cagcgcccag
     5101 accatacagt tgagcctggg cagcgcccaa agcgacctgt tggggtacct ggcactgaac
     5161 cacattaaga ttggcaagga cgtgctgcaa cagcgtaagg acgcccagac cgataccgaa
     5221 ctgacgagcg cccagaccgc cggtacgttc gacagcacct atgcccagat aatgcagtca
     5281 cagctgaccg cctaccagca gacgctgaag gcagcgtacg ccgcacaccc ggggccgaga
     5341 ggccagaagc tgctcagcga cgattaccaa gccgccgggc tgctactcgt ccagctgcaa
     5401 cagcagtaac gcttaacgtt cgctcactac caggtcgtag ctgtgccgga tgaggtcctg
     5461 cacctccggc cagctgagtt gcccggtcag taccaaggtg ttccagtgct ttttgttcag
     5521 gtggtacccc ggcatgacgc tctcgtaacg ctcccgcagc accttgctga gggccgggtc
     5581 gcatttgagg ctcaggctga ccgggtcctt gccttcggcg atgagggcga acatcttgtc
     5641 gccggtctta tagacggcca cgtctttccc gaagggatac tcgagccggc tatcgggcat
     5701 gctcagaata tatgcttcaa ctgtcttatg atccatgcgt caattgtagt acatcgcaca
     5761 ggatttaacg gtgtatagcg aagcggcaca gcgttattga cacgcttcgc tataatatgg
     5821 gttatataca agggttaagt gtttggagtg cgctatgcga tacagcctgc gagacggtgt
     5881 gacacgaaaa atcaaaaact tggtagtcaa ctcttcatta tcggccatgc tggcgatcag
     5941 cagcttaggc gtcgccttgt caatcatgct gccccagact gcgcacgccg tagccccgac
     6001 agtggtctac aatgcgctgc cgagcacatc gcccgccgca aactatccaa gccagccttt
     6061 ccaggcccaa cagaccaagg aatttggtga tgcaatccat ctggggggta cgaaccgcct
     6121 gctgaatacc gtcaccgtca c
//
LOCUS       DASSFD010000178         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_129323, whole genome shotgun sequence.
ACCESSION   DASSFD010000178 DASSFD010000000
VERSION     DASSFD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129323"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFH39_04345"
     CDS             complement(<1..377)
                     /locus_tag="VFH39_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925033.1"
                     /translation="MAKLQSVGSVAGKETIYVDVDDEITTIIDKVQGAKGKVVALVLP
                     KRATVLQSIVNMKLLKRTADDAGKNLVLVTSEAGLMPLAGSVGLFVAGTPTSKPVVPP
                     APFAPTDEPEDIDEPLQITDGSAA"
     gene            complement(437..1741)
                     /locus_tag="VFH39_04350"
     CDS             complement(437..1741)
                     /locus_tag="VFH39_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HET6925034.1"
                     /translation="MLDKFKGLKDSALSRLPAAPKGPTDTSRYMVALDIGTEYVKALI
                     GKVVDDEIKIIGVGRAHQGLSDMQAGAVADIGAVVENCDHALNDAEKQAGVSARAAII
                     GIAGELVKGTTTTVRVARKQPNKALDIAEVERIIRLVQERAEAKAKQQLAWELGGKNI
                     EVRLVNSALVGIEIDGYTVTNPIGFQGRDVVVQLYTAFAPLVHIGALERVAEQLDLDL
                     IAVAAEPFAVARSVIGNDQSNSMSAVLMDVGGGTTDIAVIHDGGVQGTKMFGIGGRAY
                     TRGVERDLGVEFDLAEKLKVGLGAGKVAARQASAVRGALGKTLEVWTSGVELALGEFT
                     KLDHLPHRVLLCGGGSSLEMLMERLQNGEWYRKLPFTRKPEVQHIHPEEVVGITDTTG
                     AVNDHTFITAMGLLRVGLDTVQYEGSGIGNTVKETIDRILHV"
     gene            1843..>2396
                     /locus_tag="VFH39_04355"
     CDS             1843..>2396
                     /locus_tag="VFH39_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925035.1"
                     /translation="MSFNPDPMEMRIHQPASSLMVTHLPTQPLQVLHQQYGDTFEQAL
                     QEHGSEETITTLENGLPLDDMVHPAEAYLDLFGATMQHADIQATYGITRDRRTLLEAS
                     LELRDRAQQTRNRLLISREIDAAYDIRLQRLNPDGSPVVPYREDLDDSEIQLHVSIAG
                     YALDCLLWQTGMAHEEAELLLPGF"
BASE COUNT          459 a          756 c          636 g          545 t
ORIGIN      
        1 gcagcgctgc catcggtaat ctgcaacggc tcatcgatgt cttccggctc gtcggtcgga
       61 gcgaacggcg ccggcggtac gaccggcttg ctggtaggcg tgccggccac gaacaggccg
      121 acactgccgg caagcggcat cagaccagct tcgctggtca ccaacaccag gttcttaccg
      181 gcatcgtccg cagtacgctt caacagtttc atgttcacga tgctctggag caccgtcgcc
      241 cgcttaggca gcacgagggc gactaccttg cctttggcgc cctggacctt gtcgataatg
      301 gtcgttattt cgtcatcgac atcgacataa attgtctctt tgccggccac gctgccgaca
      361 gattgtaatt tagccatgct gcctcccggc tatagcacgg aagaacgctg gcacagtggt
      421 ggaaagtatc atttggttac acatgtagaa ttcggtcgat tgtttctttt accgtattgc
      481 ctataccgct accttcatat tgtaccgtat ccaggccaac acgcagcagg cccatggcgg
      541 ttatgaacgt atggtcgtta acagctccgg tcgtgtcggt tatacctacg acttcttcag
      601 gatggatatg ctgtacctcg ggcttacggg tgaacggcag tttgcggtac cactcgccgt
      661 tttgcagacg ttccatcagc atttcgagcg acgaaccgcc tccacacagc agcacccggt
      721 gcggcaggtg gtccagtttg gtaaattcgc ccaatgccag ttccacaccg cttgtccata
      781 cctcgagcgt cttaccaaga gcgccgcgga ccgcagaagc ctgccgagcg gctactttac
      841 cggcccctaa gccgactttc agcttctctg ccaggtcgaa ctcaaccccc agatcgcgct
      901 cgacgccacg ggtgtatgcc cggccgccga tgccgaacat cttggtgcct tgcacgccgc
      961 cgtcgtgtat gacggctata tcggtggtgc cgcccccgac atccatcagc accgcactca
     1021 tgctgttgct ctggtcgtta ccgatgacgc tgcgggccac tgcgaacggt tcggccgcca
     1081 cggcgatcag gtcgaggtcg agctgttcgg ccactcgctc caaggccccg atgtgcacca
     1141 agggagcaaa ggcagtgtat agctgcacca ccacatctct cccctggaaa ccgatggggt
     1201 tggtaacggt atatccgtcg atttcgatac ctacaagagc gctgtttacc aatcgcactt
     1261 cgatgttctt gccacccagc tcccaggcca gctgctgttt ggctttagct tcggcccgct
     1321 cctgtaccaa gcggatgatc cgctctactt ctgcgatatc cagcgccttg ttgggctgct
     1381 tgcgagccac ccggacggta gtggtcgtgc cctttaccag ctcgccggcg atgccgatga
     1441 tggctgcccg cgcactgact cccgcctgct tttcagcgtc gttcagggcg tggtcgcagt
     1501 tttcgaccac cgcgcctata tctgctaccg cgccggcctg catgtcgctg agcccctggt
     1561 gagcacggcc cacaccgata atctttattt cgtcgtccac tactttgccg atgagggctt
     1621 ttacatattc ggtaccgata tcgagcgcca ccatgtagcg cgacgtgtct gtggggcctt
     1681 tcggtgctgc cggcaaacgg ctcagcgccg aatctttgag tcccttgaat ttatcaagca
     1741 tatgcacctt attttagcat aagcatcggc ccgccatgac cagttttgag agactgctcg
     1801 taatgttgct ataagtgcat ttttatgcta taattttatc atatgagttt caatcctgac
     1861 cctatggaaa tgcgtatcca tcagccggcc agcagcctca tggttaccca tctgcccacc
     1921 cagccgcttc aggtgctgca ccagcagtat ggcgatacct ttgagcaggc actgcaggag
     1981 cacggcagcg aagaaacgat taccaccctc gagaacggtc tgccgctaga cgacatggtg
     2041 caccccgccg aggcctacct ggacctgttc ggggccacca tgcagcacgc cgatatccag
     2101 gccacttacg gcatcacccg cgacagacgc actctgctcg aggcttcctt ggagctgcgc
     2161 gaccgtgccc agcagacgcg caaccgttta ctgatatcac gggagataga tgccgcctac
     2221 gatatccgcc tgcagcgcct gaacccggac ggttcccccg ttgtgccgta ccgcgaagat
     2281 ctcgatgact ccgagataca gctgcatgtg tctatcgccg gctatgcgct cgactgcctg
     2341 ctgtggcaaa cgggcatggc acacgaggaa gcggagttac tgctgcccgg tttcga
//
LOCUS       DASSFD010000179         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_959596, whole genome shotgun sequence.
ACCESSION   DASSFD010000179 DASSFD010000000
VERSION     DASSFD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959596"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFH39_04360"
     CDS             <1..769
                     /locus_tag="VFH39_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6925036.1"
                     /translation="EVKSQNSEVGRLVRRYHLEGQVNHFSTQLSSRLKHVSGTALSTL
                     GKIGSSIFAVLTILVLTFMMLVEGPTWIGFIRRLIPSRHRKDADTIALDMYRVVKGYV
                     NGQVLLAAIASLFIVPALFLLHGGYPFALMVVVFICGLIPMVGHTIGAIIVTTVALFH
                     SPASALIILGYYILYQQVENYLIQPRIQANSTNMSPLMVFMSVVVGVSFSGLLGGLVA
                     IPVVGCLRVLVLYYLESENKLSPVEVNKELREDYNKK"
     gene            complement(777..2186)
                     /gene="cysS"
                     /locus_tag="VFH39_04365"
     CDS             complement(777..2186)
                     /gene="cysS"
                     /locus_tag="VFH39_04365"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6925037.1"
                     /translation="MKLYNTLTRSVEAIKPLQPPTVTVYTCGPTVYDYPHVGNWFTYI
                     REDILIRTLKASGFQPKWVMNITDVGHLVSDADEGEDKLEKGARREGKTAWDVAERYT
                     DYFITGLERLNISQPDYLPKATDHIDEQIELVKVLETKGYTYIISDGVYFDTSKFPTY
                     PDFARLDIDEQQAGARVQHNPEKRNSSDFALWKFSPHDHRRDMEWDSPWGKGFPGWHI
                     ECSAMSMKYLGDTLDIHAGGIDHLPVHHTNEIAQSEAATGKTFALHWMHSNHITIEGE
                     KVSKSLGNGITLEDIEAKGISLAAFRLHVLESHYRSQSKFSWESLEAAHNRLKHWQTV
                     ADLRLQPQAAVQRDVSLEAGAAKAAHDILQALQDDLGTPQALARIDSFFGATELTGVL
                     PGETKDFTDLLQNVRDWLGIDLLQSGDITDEQKRIISEREQARTDKEWAKSDELRDTL
                     IQQGIGLRDTPNGAVWYRL"
     gene            complement(2158..3192)
                     /locus_tag="VFH39_04370"
     CDS             complement(2158..3192)
                     /locus_tag="VFH39_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET6925038.1"
                     /translation="MQDKIKELVDGAQRIVVVQADNPDADSLGSALSLEHILGDLGKD
                     VAMYCGVDVPTYLRYLTGWGRIVNELPSSFDLSIVVDASTYTLLEKLEQSGQLGWLKP
                     KPCIVLDHHATVERPIDFATVSLVEPKAASTGQLIYELAHSLGWKVNKTAAGHIMSSI
                     LGDTQGLMNYQTTAQTYRVMADLTDLGADRPLLEELRREYSRMAQDVFRYKGELIRRL
                     DFAADGRIAHVSIPQAEINEYSPHYNPAVLIQFDSLQVNSVQLSIVFKTYDDGRITGK
                     LRSNLIAPVAGKLAEHMGGGGHPYSAGFKVTDGRPFNEVKSECLAFATELLDNLDKDA
                     PDEALQHPDA"
     gene            complement(3224..>3793)
                     /gene="recR"
                     /locus_tag="VFH39_04375"
     CDS             complement(3224..>3793)
                     /gene="recR"
                     /locus_tag="VFH39_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET6925039.1"
                     /translation="DALSNLPGVGPRTAERYAYYLLRRDAHTSQQLADAVANLHAGVS
                     YCRKTFALVPAGQELSDLYTDSKRDKTTVAVVAEPFDVLALEKTGQYHGTYHVLGGLV
                     SPIDGITPDQLHIAELISRVDEDQVKEIILATNASVEGESTALYIQQQLGDKPVKVTR
                     LARGLPIGVDLEYADQITLGRALEGRQAL"
BASE COUNT          711 a         1042 c         1123 g          917 t
ORIGIN      
        1 agaagtaaaa agccagaaca gcgaggtggg tcgcctggtg cgtcgctatc atctggaggg
       61 ccaggtcaat cacttctcca cccagttgtc ctcgcggctg aagcatgtca gtgggacggc
      121 gcttagcaca ctaggcaaga tcggcagcag tatctttgcc gtgctgacga tattggtatt
      181 aacctttatg atgctggtcg aaggtcctac ctggatcggc ttcatacggc gtctgatacc
      241 cagccggcat cgcaaggacg ccgacaccat agcgcttgat atgtaccgtg tggtcaaagg
      301 ctacgtgaac gggcaggtcc tgcttgcagc aatagcgtca ttgttcatcg taccggcact
      361 gttcctactg cacggcggct acccgttcgc cctgatggtc gtggtgttta tctgcggtct
      421 gataccgatg gtgggccaca cgataggcgc gattatagtt acgaccgtcg ccttgttcca
      481 ttcgccggcg tcggcgctga tcatcctcgg ttactatatc ttgtatcaac aagttgaaaa
      541 ctacttgata cagccgcgca tccaagccaa ctcgaccaat atgtcaccac tcatggtgtt
      601 catgtcggtt gtcgtcggtg tcagcttcag cggcctctta ggtggtctag tagccatccc
      661 cgtggttggc tgcctacggg tgctggtgct gtactatctc gaatcagaga ataagctcag
      721 tccggtcgaa gtcaacaaag aactccgcga agactataac aagaaatagc cgctggctac
      781 agtcggtacc agacagcgcc gttgggcgta tcgcgcagac ctatcccttg ctggatcagc
      841 gtgtcacgca gttcgtcgga tttggcccac tccttatcgg ttctcgcttg ctcgcgttca
      901 ctaatgatgc gtttctgctc atctgttatg tcgccgcttt gcaataagtc gatgcccagc
      961 cagtcccgca cgttctgcag caaatccgta aagtctttcg tttcgccagg cagcacgcct
     1021 gtcagctccg tggcgccgaa aaagctgtcg atccgggcga gagcttgggg tgtaccgaga
     1081 tcatcttgca gggcttgcaa aatgtcgtgg gcggccttcg ctgcgcccgc ctctaggctg
     1141 acgtcccgct ggactgcggc ctgcggctga agccgcaagt cggcgaccgt ctgccagtgc
     1201 tttaagcggt tatgcgcagc ctccaggctt tcccagctga atttgctttg gctgcggtag
     1261 tgactctcaa gcacatgtaa gcggaaggcg gccagactta tgcccttagc ttcgatatct
     1321 tcaagcgtaa tgccgttgcc caggctcttg ctgaccttct cgccttcgat ggtgatgtgg
     1381 ttgctgtgca tccaatgtaa ggcgaatgtc ttgccggtgg cagcctcgga ctgggcgatc
     1441 tcgttggtgt gatgaaccgg caagtggtcg atgccaccgg cgtggatgtc cagcgtgtca
     1501 cccaggtact tcatgctcat ggctgagcac tctatgtgcc agcccggaaa gcctttgccc
     1561 cacggactgt cccattccat gtcgcggcgg tgatcatgcg ggctgaactt ccatagcgcg
     1621 aagtcgctgc tattgcgctt ttcgggattg tgttggacac gggcgccggc ttgctgttca
     1681 tctatgtcca aacgggcgaa gtcaggatat gtaggaaatt ttgaggtgtc gaagtagacg
     1741 ccatcggaga tgatgtatgt ataccccttt gtttccagta cctttaccag ctcgatctgt
     1801 tcgtctatgt ggtcggtcgc cttcggcagg tagtcaggct ggctgatgtt cagacgctcc
     1861 aggccggtta tgaaataatc ggtgtagcgc tcggccacgt cccaggccgt cttgccttcc
     1921 cgacgggcgc ctttttcgag cttgtcctcg ccttcatcgg catcgctgac caagtggccg
     1981 acgtcggtga tattcatgac ccatttcggt tgaaagccgc tggctttcag ggtgcggata
     2041 agaatatctt cgcggatgta ggtgaaccag ttgccgacgt gcgggtagtc atagaccgtc
     2101 gggccgcagg tatacacggt gaccgtcggc ggctgcagcg gcttaatggc ttcgacgcta
     2161 cgcgtcaggg tgttgtagag cttcatcggg agcgtccttg tctaaattat cgaggagttc
     2221 ggtggcgaag gcaagacact cgcttttgac ttcgttgaac ggccggccgt cggtcacttt
     2281 gaagccggcg ctgtaggggt ggccgccgcc gcccatatgc tcggccagct tgccggcgac
     2341 gggtgcgatc agattactgc gcagtttgcc agtgatgcgg ccgtcgtcgt atgttttaaa
     2401 gacgattgat agctgcacgc tgttgacttg cagtgaatcg aactgaatca atacggctgg
     2461 attgtagtgg gggctgtact cgttgatctc ggcctgcggt atggagacgt gcgctatgcg
     2521 tccgtcggcg gcaaagtcca ggcggcggat gagctcgcct ttgtaacgga acacgtcctg
     2581 ggccatgcgg ctgtattcgc gtcgcaattc ctccagcaac gggcgatcgg cgcccaggtc
     2641 ggtcagatcg gccatgaccc ggtaagtctg ggcggtcgtc tggtagttca tcagaccctg
     2701 ggtgtcaccg agtatcgagc tcatgatgtg ccccgcggcc gtcttattga ccttccatcc
     2761 tagtgagtgg gccagttcgt atatcaattg gccggtcgag gccgccttcg gctcgaccag
     2821 ggagacggtc gcaaaatcta tgggccgctc gaccgtagca tggtgatcga gaacgataca
     2881 tggcttgggc ttgagccagc cgagctgacc gctttgttcc aacttttcca gcaaggtgta
     2941 agtgctggca tcgacgacga tggataggtc gaaactgctg gggagttcat tcacgatccg
     3001 gccccagccg gtcagatatc gcaaataggt cggcacgtcg acgccacaat acatggcgac
     3061 atctttgcct agatcgccca gtatgtgctc gagcgataag gcactgccga gactgtccgc
     3121 atcgggatta tcggcctgta cgacgacgat acgctgggca ccatcgacca attctttgat
     3181 tttatcttgc attctgtatc gattgtacgg tgcttgagcg gttttataaa gcttggcgac
     3241 cttctagggc gcggccgagg gtgatctggt cggcgtattc caggtcgacg ccgataggta
     3301 agccacgtgc cagccgcgtg actttcaccg gtttatcccc cagctgctgc tggatataca
     3361 gagccgtgga ctcaccttcc acgctggcat tggtcgccag aatgatttcc ttgacctggt
     3421 cttcgtccac ccggctgatc agctcggcga tatgcagctg gtccggtgta ataccgtcga
     3481 tcggggagac caggccgccc agcacatggt aggtgccgtg atactggccg gtcttctcga
     3541 gcgcgaggac atcgaacggt tcagcgacga ccgccacggt ggttttatcg cgcttagaat
     3601 cggtgtacag atctgacagt tcctgtcctg ccggcactag ggcgaacgtt ttacgacagt
     3661 agctgacgcc agcatgcagg ttggctacgg cgtcggccag ctgctgactg gtgtgcgcgt
     3721 cacggcgcag cagataatac gcatagcgct cggctgtccg tggaccgaca cccggtaggt
     3781 tcgagagcgc atc
//
LOCUS       DASSFD010000180         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_831394, whole genome shotgun sequence.
ACCESSION   DASSFD010000180 DASSFD010000000
VERSION     DASSFD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831394"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2293)
                     /locus_tag="VFH39_04380"
     CDS             complement(<1..2293)
                     /locus_tag="VFH39_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET6925040.1"
                     /translation="MAKTTKPTATKTNKHQDRVYFTSDDSALPLPSLVDHQNKSFQWF
                     VEEGLGELLAEISPIDDYTGAKLSLSFKDYHFEDPKMTESEARENNVSYDAPLKATVE
                     LTNKVTGEVKEQEIYLGDYPWMTSRGTFVINGAERVVVSQLIRSAGVFFTADPHGTTS
                     LYGAKVIPGRGAWLEFETAANGALYVKIDRKRKIAVTTLLRALGVTETRMREAFKHVD
                     QGKLSYLDATLEKDPTRGQNDALIEVYRRLRPGDLATVDNARSLLENMFYNFKRFDLS
                     RVGRYKLNKRLNLDVPNTMENRTMRLEDLEAIIAEVIRLNNTQEPADDIDSLANRRVK
                     LVGELVQRQFRIGLLRMERNTKDRMSMSEIETVQPGQLINARPVVAAVREFFASSQLS
                     QFMDQINPLSELAHKRRLSSMGPGGLSRERAGFEVRDAHATHYGRICAVETPEGANIG
                     LVLNLANYARVNEYGFVETPYRKVVNAATAKDIVGHIAAEDYVDEKGKVLVKAGAKLT
                     AADAAKLAKVTSKVTWPVKARVTNEVVYLDAYEEESATIAGAGSQLDEDGYFLEERVS
                     GRQYLKSSEVDANDVQYMDAARNQIIGSSAGLIPFIEKNYVYRSLMGSNQQRQAVPLI
                     QPQSPIVGTGLEATAAQNTGQVMLAEADGEVVSASADEVKVKYASGTVSYEPQHFVRS
                     NEGTSINQKVVVSTGDKVKQGDVLVEGMSIDGGELALGKDLRVAFMPWGGYNFEDAII
                     ISRKLVEDDTLTSIHIVDFMIEVR"
BASE COUNT          416 a          713 c          791 g          583 t
ORIGIN      
        1 cgcggacctc gatcatgaag tcgacgatgt ggatgctggt cagggtgtcg tcctcgacga
       61 gcttgcggct gatgatgatg gcgtcctcga agttgtaacc gccccatggc atgaaggcca
      121 cacgcaggtc tttgccgagt gccagctcac cgccgtcgat gctcatacct tccacgagta
      181 catcgccctg cttcaccttg tcgccggttg aaacgacgac tttctggttg atgctggtgc
      241 cttcgttact gcgcacgaag tgctgcggct cgtagctgac ggtaccggag gcatacttga
      301 ctttgacttc gtcggcgctg gcgctgacga cttcgccgtc ggcttcggcc agcatgacct
      361 gaccggtgtt ctgggccgcg gtggcttcca ggcccgtacc cacgattggg ctctgcggct
      421 gaatcagcgg cactgcctga cgctgctggt tactacccat cagcgagcgg taaacgtagt
      481 tcttttcgat gaacgggatg aggccggcgc tactaccgat gatctggttg cgggcggcgt
      541 ccatgtattg gacgtcgttg gcgtcgactt cgctggactt caggtactga cgaccgctga
      601 cgcgctcctc caggaagtag ccgtcttcgt cgagctggct accggcgccg gcaatggtgg
      661 ccgactcttc ttcgtaggca tccaggtaga ccacttcgtt ggtcacgcgg gccttgaccg
      721 gccacgtcac cttggaagtg accttggcca gcttggcggc gtcggcggca gtcagcttgg
      781 caccggcttt caccagcact ttgccctttt cgtccacgta atcttcggcg gcgatatggc
      841 cgacaatgtc cttggccgtc gcggcgttga ccactttgcg gtacggggtc tcgacgaagc
      901 cgtactcgtt cacgcgggca tagttggcga ggttcagcac cagaccgatg ttggcgcctt
      961 ccggcgtctc gacggcacag atacggccgt agtgggtggc gtgggcgtcg cggacttcga
     1021 agccggcacg ctcacggctc aaaccaccgg gacccatgga ggaaagacgg cgcttgtggg
     1081 ccagctcgct cagggggttg atctggtcca tgaactggct gagctgggag ctcgcgaaga
     1141 attcgcgaac agccgccacg accggacggg cattgatcag ctgacccggc tgcacggtct
     1201 cgatttcgct catactcatg cggtccttcg tgttgcgctc catgcgcagc aggccgatgc
     1261 ggaactgacg ttggacaagt tcaccaacga gcttgacgcg gcggttggcg agcgagtcga
     1321 tgtcgtctgc cggctcctgc gtgttgttga ggcggatgac ctcggcgata atagcctcca
     1381 ggtcctcgag ccgcatggtg cggttttcca tggtgttcgg cacatccagg ttgaggcgct
     1441 tgttcagctt ataacggccg acgcgggaga gatcgaagcg cttgaagttg tagaacatgt
     1501 tttcgagcaa agagcgggcg ttgtcgacgg tcgccaggtc gcccgggcgc agacggcggt
     1561 agacctcgat cagggcgtcg ttctggccgc gggtcgggtc tttttcgagc gtcgcatcca
     1621 ggtaactgag cttaccctgg tcgacgtgct tgaaggcttc gcgcatgcgg gtttcggtga
     1681 cgccgagcgc acgcagcagt gtggtgacgg cgatcttgcg cttgcggtcg atctttacgt
     1741 acagcgcacc gttggcggcc gtctcgaact ctagccaggc accacggccg gggatgactt
     1801 tggcaccgta caggctggtc gtcccgtgcg ggtcggcagt gaagaacaca ccggccgagc
     1861 ggatcagctg actgaccacg acgcgctccg caccgttgat aacgaacgta ccgcggctgg
     1921 tcatccacgg gtagtcaccg aggtagattt cctgttcttt gacttcgccg gttactttgt
     1981 tggtcagttc gacggtcgct ttgagcggcg catcgtagct gacattgttc tcccgagcct
     2041 cagactcggt catcttgggg tcttcgaagt gatagtcttt gaagctcagg ctgagcttcg
     2101 cgccggtata gtcatcaatc ggactaatct cggccagcag ctcgccgagg ccttcctcga
     2161 cgaaccattg gaatgattta ttctggtgat cgaccaggct cggcagcggt aatgcgctgt
     2221 cgtcggatgt gaagtacacc cggtcctgat gtttgttggt tttggttgcg gtcggcttgg
     2281 tggtcttggc catgcgtgcg tatgccccct tggttacgtt attgctcgtc gcaccgtctc
     2341 tttagcagca ggccgcgtgg tcactgacca cacaacgagc tacgctgaaa acggctttat
     2401 ggttacgtgt tggtgttagt ggaatttata agatttggcg ccgaagatcc taagtcgggt
     2461 gtctttggtt cgcgcgtctc tcagcacgca aactacccag ctt
//
LOCUS       DASSFD010000181         3596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_199588, whole genome shotgun sequence.
ACCESSION   DASSFD010000181 DASSFD010000000
VERSION     DASSFD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199588"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFH39_04385"
     CDS             complement(<1..784)
                     /locus_tag="VFH39_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925041.1"
                     /translation="MWALTKQSFRKSPGHRLDQSDGSIVVTILTLTAFLSLSMMAMAV
                     MATSNVTRARGRILLLQAQYSAESGADAAIATLNNTSSDYTGTGGTEVTVLSTPQYRS
                     TYTTTVSTDANPKERIIVATGKVYAPASSTSPRYSRTIRVTADRSSTTSAASVVSRNI
                     LTIASSVKQVYAKDIYVNGYINMVKNTTTLYAENITVGGRDTGAGNCSIEGGGSLAKP
                     SSFSTAGQTKTIIKLAYNNCINPPGNISNTDFDVSANQTNIST"
     gene            complement(788..1564)
                     /locus_tag="VFH39_04390"
     CDS             complement(788..1564)
                     /locus_tag="VFH39_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET6925042.1"
                     /translation="MMRRVNQGGFTIPELIIMMIVTTFLTLLVITFALNFWSSSATLQ
                     NDSDALTTRLNTGDLLREQLNAASNLINQNSIQDPNAGDADPTAGPAYWLLLHAVPQT
                     VSMPTSGITPVFYFQAPSVDAARNFIMNGSQPYQDEFVLYMNGANKELLLRTLVNPAA
                     TGDRLKTSCPPALATASCPADKVMGVDVSSVALRYFSRSGNTIDYTSVVDPVYGDYIG
                     PDFEAVEVVELTVNVHKKSIIHGGNDTSSEVIVRVALRNG"
     gene            complement(1561..2067)
                     /locus_tag="VFH39_04395"
     CDS             complement(1561..2067)
                     /locus_tag="VFH39_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET6925043.1"
                     /translation="MTRIVSLRRLKQRRPGGGLSMRRRDQGGFTIVEAIIGCVVFGII
                     AGVMLNSYEGLRASYVTARQLNEIYTVLSACPEVDRALEFTSLSSTSNCYPNNSFPAE
                     DGGSGTITYTPSLSVTNTSALGNTDPLYGIPDSKVVKIDVGYPRPNTSAPHLQLRMLI
                     TRNGVGQL"
     gene            2190..2336
                     /locus_tag="VFH39_04400"
     CDS             2190..2336
                     /locus_tag="VFH39_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HET6925044.1"
                     /translation="MAHKNPGNFANNRARAQAAGRKGGSVSPGNFKFDPARASRAGRK
                     GGLK"
     gene            complement(2414..2674)
                     /locus_tag="VFH39_04405"
     CDS             complement(2414..2674)
                     /locus_tag="VFH39_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET6925045.1"
                     /translation="MLILGAIVGWLAAAIMGRDEGIFASIVIGVVGSIIGGLLSMLFT
                     GGNHAALAFTWGGLLWSLVGAIILVAILNAVQGRRHHRVGHM"
     gene            complement(2918..3391)
                     /locus_tag="VFH39_04410"
     CDS             complement(2918..3391)
                     /locus_tag="VFH39_04410"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET6925046.1"
                     /translation="MIRHLVAVDSKRGIAKNGVQPWKLPGDEAYFTEMSKRYGGNVLM
                     GRTTYDVIGHPLPERRNFVASRHMSETDGVQVVQDIDAFLAAFPEDIWVIGGADIYRA
                     TMEQAEELYVTEIEADFDCDRFYPVIGAEFRLDRRSQVQIENRYRYTYAVYVRRT"
     gene            complement(3388..>3596)
                     /locus_tag="VFH39_04415"
     CDS             complement(3388..>3596)
                     /locus_tag="VFH39_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925047.1"
                     /translation="SEKDFPKHSVPLEIVKDYARLNDYYIEEIPDPVAALGALQQRSE
                     PVLIVCGSFYLMNHIRPLLLEKAV"
BASE COUNT          776 a         1003 c          999 g          818 t
ORIGIN      
        1 cggtggaaat attcgtttgg ttcgccgata cgtcgaagtc ggtgttgctg atattccctg
       61 gcggattgat acagttgtta taggccagct taatgatcgt cttggtctga ccggccgtcg
      121 agaagctgct cggcttagcg agactgccac ccccctcgat cgaacagtta ccggcacccg
      181 tatcacggcc gccaaccgtt atgttttcgg catagagcgt agtggtgttc tttaccatat
      241 tgatataacc gttcacataa atgtctttgg catacacctg cttaactgaa ctggcgatgg
      301 tcaggatatt acggctcacc accgaagcgg cgctagtggt agaacttcgg tcggcagtga
      361 cgcggatcgt ccgtgagtag cgtggcgagg tcgaactggc tggcgcatag acttttccgg
      421 ttgccacgat gatgcgttct tttgggttgg catccgtact gaccgtggtg gtataagtgc
      481 tccggtactg cggcgtgctg agcacggtta cttcagtacc gccggtgccg gtgtagtcgg
      541 aactggtgtt attgagtgtc gctatggcgg cgtcggcgcc ggattcggcc gaatattgcg
      601 cctgcaacag cagaatgcgg ccgcgcgccc gggtgacatt gctggttgcc atcaccgcca
      661 tggccatcat gctgagtgat agaaacgcgg tcagcgtcag gatggtcact actattgaac
      721 cgtcgctctg gtccaaccgg tggccgggcg actttctaaa agattgcttc gtgagtgccc
      781 acatgtccta cccgttcctc agtgctaccc gcacaattac ttcgctggag gtatcattgc
      841 ccccatgaat gattgatttc ttgtggacat tgaccgttaa ctccaccact tcaaccgcct
      901 cgaagtccgg cccgatataa tcaccataga ccgggtcgac gacagacgtg taatcgatgg
      961 tgttgccgga gcgagagaag taacggagag ccaccgacga aacatccacg cccatgactt
     1021 tgtcggccgg gcacgaggcg gtcgccagcg ccggcggaca gctcgtcttc agccgatcgc
     1081 ccgttgccgc cgggtttacc agcgtgcgca gcagcagctc tttgttggca ccgttcatat
     1141 acagtacaaa ctcgtcctga tagggctgtg acccgttcat aatgaagtta cgggccgcat
     1201 cgacgctggg tgcctgaaaa tagaagacgg gtgtgatgcc gctggtcggc atactgacgg
     1261 tctgcggcac ggcatgcaac agcaaccagt aggccggccc agcggtggga tcggcatcgc
     1321 cggcgttcgg gtcctgaatg ctgttctgat ttatcaggtt ggaagcggcg ttcaactgct
     1381 cgcgcagcag gtcaccggta ttcaggcgcg tcgtcaacgc gtcggaatcg ttctgcaatg
     1441 tcgcgctgct gctccagaag ttcaaggcga atgtgatgac gagcagcgtc aggaatgtag
     1501 tgacaatcat catgatgatc agctccggga tagtgaagcc gccttggttc acccgtcgca
     1561 tcataattgc cccaccccgt tacgggtaat gagcatacgc agctgcaagt gcggggcgga
     1621 agtgttcggc cgcgggtagc cgacatctat cttcaccacc ttggaatccg gtatgccgta
     1681 gagcgggtcg gtgttaccca gcgcgctggt attcgtaact gacaggctgg gcgtataggt
     1741 aatggtgccg ctgccgccgt cttcggcagg aaatgaattg ttcggatagc agttcgaggt
     1801 gctgctgagc gaggtaaatt caagcgcccg gtcaacctcc ggacaagcac tgagcacggt
     1861 atatatttcg ttcagttgcc gcgccgtcac atatgatgct cgcagccctt cgtaggagtt
     1921 caacataacg ccggcgatga taccgaatac gacacagccg atgatcgcct ccacaatggt
     1981 gaagccgcct tggtcgcggc ggcgcatcga caatccccca ccgggccgtc gctgcttcag
     2041 gcgccttaaa ctgacaatac gtgtcatgct tatgattatt atatacccca gcggcgcggt
     2101 aaaacgtgac cgtcagtggt atttctcata gctcaccttc agcaaagcgg tttatgctta
     2161 tgaatgctaa ttaacctaag gaggagtaga tggcacacaa gaatcctgga aactttgcga
     2221 ataatcgggc ccgcgcacag gctgcaggcc gcaagggcgg ctcggtaagt cctggtaatt
     2281 tcaagttcga tccggctcgc gccagccgcg ccggccgtaa gggcggtctg aagtagcgga
     2341 ccgctactgg tatcgcccaa tgaaaactgc tccccttcgg gagcagtttt catatcagct
     2401 gatagaagaa gctttacatg tgtccgacgc ggtgatgcct gcggccttgc acagcgttaa
     2461 gaatggcaac taagatgatt gcgccgacca atgaccacag caggccgccc catgtaaacg
     2521 ccaaggcagc gtgattacca cccgtgaaca acatggacag caggccaccg ataatcgatc
     2581 cgaccacacc tatgacgatg ctggcgaaga taccttcgtc gcgtcccatg attgctgctg
     2641 ccagccagcc tacgatcgca cccagaatta acatcacaat aaggcccata gacttctcct
     2701 ccgtttatat ttcgactgac taacgcgcta agctcgactg tcaagcagga acgaccgggt
     2761 gtacccggaa attgcaggat gccttgtcag atcgtgacac gaatcgacaa aatttctgct
     2821 catcgcttct ccctttccgt taagcttcta tgcctatggt atagggcggc cgcaggagcc
     2881 actattaatt atcttacata gcggagcgtg agcgcagtca cgtgcggcgc acatacacgg
     2941 catacgtata gcggtacctg ttttcgattt gtacctggct gcgccgatcc aaacggaact
     3001 ctgctcctat taccgggtaa aagcggtcgc agtcaaagtc ggcttcgatt tccgtcacgt
     3061 acagttcctc cgcttgttcc attgtggccc ggtagatatc cgctccgccg ataacccata
     3121 tatcttcagg gaaggccgcc aggaaagcat caatgtcctg caccacctgc accccgtccg
     3181 tttcagacat atgccggctg gccacgaagt tccggcgctc gggcaaagga tggccgatga
     3241 cgtcgtaggt ggtgcggccc atcaatacat tgccgccgta gcgcttcgac atttcagtaa
     3301 agtacgcttc gtcacccggc agtttccacg gctgcacgcc gtttttggca ataccgcgct
     3361 tgctgtcgac ggccaccagg tgccggatca taccgctttc tccagtagca gcgggcggat
     3421 atggttcatc agataaaatg aaccacaaac aataagtacg ggttcagagc gttgttgcag
     3481 cgcccccagc gcggcgaccg ggtcgggaat ctcctcgatg tagtaatcgt tcagccgggc
     3541 gtaatctttt actatctcca gcggtaccga gtgcttggga aagtctttct cgctgt
//
LOCUS       DASSFD010000182         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_702507, whole genome shotgun sequence.
ACCESSION   DASSFD010000182 DASSFD010000000
VERSION     DASSFD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702507"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFH39_04420"
     CDS             <1..765
                     /locus_tag="VFH39_04420"
                     /inference="COORDINATES: protein motif:HMM:NF045158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925048.1"
                     /translation="ISRRTAVPDFIGGDPESNASECPAVFVIPETGDFVFRGKTITDP
                     ALIAELNEHIGKAEDESDVWLPARMAPFIRDALDGDEQGRQGPGQHTFAELLAATQAS
                     VIRFEMRDSYDESEQGFAEWKATGDIGSYDWGDHLDVIRAAVARGVTVRRVRVVSEPL
                     SDYLRWEHACTAVNIEAGEDIRWLPRTKAADLMLPGADCFVFDHRVVRWNFQRGDGTN
                     PRQYTFSSDPRTIRDIVAAFEMAWNRATPHADYKPD"
     gene            complement(906..1745)
                     /locus_tag="VFH39_04425"
     CDS             complement(906..1745)
                     /locus_tag="VFH39_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925049.1"
                     /translation="MPFESLSCQRCGSPDIQEVKPETYFCNHCDNVFKYVSPKGAGSA
                     GGCELPTGGKSCGVPAVGHCNTCGRAFCSTHQAYDQNHIGVVFRTYRDWCVSCQQQRK
                     VDERNLAESKEKQRKEERAAAAERIPVLIAQFKAQPFTGAVSRDYVEQVWSGTKFLSS
                     ARKYKSVTHRYEPAVPLGELLWDYIKVGFDNDPNKQDTALWKTGLTQKGEFVPMDTSL
                     FHSIFVASGLGYNLTRGQEVKICECLERLLGNASQESKTAAAQDPRFKLTRDGKVNYA
                     DPK"
     gene            complement(2186..>3589)
                     /locus_tag="VFH39_04430"
     CDS             complement(2186..>3589)
                     /locus_tag="VFH39_04430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6925050.1"
                     /translation="ITVVHDVGSDNGQLFIVMELLHGRDLATILAGAPAGLPIDTIAS
                     LTIQAAEALRVAHAGHVVHRDLKPANLFVQDGERLKICDFGIARAVDSTAHFTATGQV
                     IGTPAYMSPEQWRGQHVDERSDLYSLGCVLYALLTGQPSFAEGQPHAIMYEHLNTVPA
                     APRTIRQDVPREFDHLVLELLAKDPARRPADVGHIIAVLQEWRYTPTVKVEPTGKAST
                     RLDGGAPEYRENAVGFQPTLNGFEGTPPAQPTRIRRVPRTETERQQVLLARDDYWEFF
                     YFAGQLLYERDGAEAKYRDPGIRYSLPNAEAVTAEDIASYTELINQRSDDARSLANEA
                     DVIINMNDRAAIDYAFGPPGQEGDPERLAQLAKRWNGFYVGFLDWAARVRSISASPEL
                     QKLLELAARFADEPLEKYRQFVDKYVAQVNEFPRLIAAGEQLRIEGSIVLVSPDEAIR
                     DYTAELNYLRSRFRLWL"
BASE COUNT          745 a         1087 c         1029 g          728 t
ORIGIN      
        1 atctccagga ggactgcagt gcctgacttc atcggcggcg accccgaatc caacgccagc
       61 gaatgcccgg ccgtgttcgt catcccggag acgggagact tcgtgttccg cggcaagacc
      121 atcaccgacc cggcgctcat cgccgagctc aacgagcaca tcggcaaggc cgaggacgag
      181 tcggacgtgt ggcttccggc tcgcatggcg ccgttcatcc gggacgccct ggacggggac
      241 gagcagggcc ggcaaggccc cggccagcac accttcgccg agttgctggc cgccacgcag
      301 gcatccgtta tccgcttcga gatgcgggac tcctacgacg agagcgaaca gggattcgcc
      361 gaatggaagg cgactggcga tatcggcagc tacgactggg gtgaccacct cgatgtcatc
      421 cgcgccgcgg tggcacgcgg cgttacggtg cgccgggtcc gggtcgtgtc ggaacccctg
      481 tcggactacc tgcggtggga gcacgcttgc accgcggtca atatcgaggc cggggaagat
      541 atccgctggc taccccgcac caaggccgcc gacctgatgc tgcccggcgc cgactgcttc
      601 gtgttcgacc accgggtcgt gcggtggaac ttccagcggg gggacggtac caacccccgt
      661 cagtacacct tcagttcgga tccccgaacc atccgcgata tcgtcgccgc gttcgagatg
      721 gcgtggaacc gggcaacccc gcacgccgac tacaagcccg actgaaacga actggccccg
      781 tctcaaggtg cacctatacc tgcggcggct acgaaccaga cgttacgaag cgcatggcct
      841 gcaaaccggt tagggacccg ctcccgctgg ccgtgaaagc ctaagcggag gaagctgccg
      901 cttagctatt tagggtccgc gtagttcacc tttccatctc tcgtgagctt gaaccgtggg
      961 tcttgggccg ctgccgtctt agactcttgg ctggcgttgc ccagcagacg ttctagacac
     1021 tcacagatct tgacctcctg tcctctcgta aggttatacc caagccccga cgcaacgaaa
     1081 atgctatgga agaggctggt atccattggg acgaactcgc ccttctgagt cagccctgtt
     1141 ttccatagag cagtatcttg tttgttagga tcgttatcaa agcccacttt aatataatcc
     1201 cagaggagct cgcccagtgg cactgccggc tcataccggt gagtcactga cttgtactta
     1261 cgagcagagg acagaaattt tgtgccactc caaacttgct caacgtaatc gcggctaacc
     1321 gcaccggtga acggttgcgc cttgaactgt gcaatgagca ccggaatacg ctcagcggcg
     1381 gccgctcgct cctctttacg ttgcttttct ttggattcgg cgaggtttcg ttcgtcgact
     1441 ttgcgctgtt gctggcatga gacgcaccaa tcccggtacg ttcggaatac aacgccgatg
     1501 tgattctgat cgtaggcctg atgcgtggaa cagaacgccc ggccacacgt gttacaatgc
     1561 cctacagccg ggaccccgca ggactttccc ccagtcggaa gctcacaacc accggccgat
     1621 cctgcccctt tcggactgac gtatttgaaa acgttgtcgc aatgattaca gaagtatgtc
     1681 tcgggtttga cttcctgaat atccggagag ccgcagcgct ggcacgacaa cgattcaaat
     1741 ggcatacctt tccccctaca ttagttgcgg atcgaggtgg ttgacgaaat ctgcggtcgg
     1801 aaggagatca tcaggacatg gtatctgagt tgactccgcg atgaaaggac gttcaaagcc
     1861 taacgtcgca ccatgccgcg gccggacccg ttgccccaca gagtccaccg atctggccgt
     1921 tgtggaagag gtgcccgggt aggcggtcac ctcgtacagc agcgagatac agcaacctat
     1981 gcgcgcaatg gcggcctgga caggcatcgg cacacgccag gctgcaccgt ttggccgcga
     2041 gcagggccac gctggtagtt tgcatccaga aggccggtgc tctggagacc agacgtggat
     2101 cgagcaccag gtctgctcct gatcccggca actgaacaca ccgccagcct acagacttcg
     2161 gagccgtgag cgctcatcaa agcttctata accaaagccg aaagcgactt ctaaggtaat
     2221 tcaattcagc tgtgtaatcc cttatagcct catctgggct aacaagcaca atagacccct
     2281 ctatgcgcag ctgctctccc gcggcgataa gtcttggaaa ctcattcact tgggcgacat
     2341 atttgtccac aaactgccgg tacttttcca atggttcgtc tgcaaaccgg gcggccagct
     2401 ctagcagttt ctgcagttca ggcgatgcac tgatactcct gactctggca gcccaatcca
     2461 gaaatcccac atagaagccg ttccaccgct tcgcaagctg cgcgagtctc tcaggatcgc
     2521 cttcttgtcc aggcggaccg aaagcataat cgatggctgc cctatcgttc atattgatga
     2581 taacgtccgc ctcatttgcg aggctcctgg catcgtcgga ccgctgatta atcaactcag
     2641 tatagctagc gatatcttca gccgtaacgg cctcggcgtt aggcagagaa taacggatcc
     2701 cagggtcccg gtacttcgct tcagcaccgt cgcgctcata tagtaattga ccggcgaaat
     2761 aaaagaactc ccaataatca tcgcgcgcta ggagaacttg ttgccgctcg gtctccgttc
     2821 gtggtacacg tctgatcctg gtcggttgtg ctggtggagt gccttcaaat ccgttcaggg
     2881 tcggctggaa gccaaccgcg ttttcgcggt actcaggcgc gccgccatca agccgggtgg
     2941 aagctttccc ggtgggctca actttgaccg tcggcgtata acgccattcc tgcaagactg
     3001 cgatgatatg gccgacgtca gccgggcggc gtgccggatc cttggcgagc agttccaaga
     3061 cgagatgatc gaactctcgg ggaacatcct ggcggattgt ccgcggcgcc gcaggcaccg
     3121 tgttgaggtg ctcgtacatg atcgcatgcg gctgaccttc tgcgaaggat ggctggccgg
     3181 tgagcagcgc ataaagcacg cagcccaggg agtacaagtc gctgcgctcg tcgacatgct
     3241 gtccccgcca ttgctcgggg gacatgtagg caggagtacc gatgacctga ccggtggccg
     3301 tgaagtgggc cgtggaatcg acggctcggg caattccgaa gtcgcagatc ttcaaccgct
     3361 cgccgtcttg gacaaacaag ttagcgggtt tgaggtcccg gtggaccaca tgaccagcat
     3421 gcgcgactcg gagtgcttca gccgcctgga tagtcagcga agcgatcgta tcgataggca
     3481 ggccagcggg tgcccctgcc aatatcgtgg ccagatcgcg gccatgcaac agttccatga
     3541 cgatgaacag ctgaccgttg tcggagccaa cgtcatgcac cacggtgat
//
LOCUS       DASSFD010000183         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_90606, whole genome shotgun sequence.
ACCESSION   DASSFD010000183 DASSFD010000000
VERSION     DASSFD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90606"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="tig"
                     /locus_tag="VFH39_04435"
     CDS             <1..1039
                     /gene="tig"
                     /locus_tag="VFH39_04435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET6925051.1"
                     /translation="SIRPVNQPQVSVTKFVPYSDLEADYEVNVIGDITMPDYKKFRLP
                     KPNVKVETKDVDEVMSNLRQRSATKQVVERAAKDGDEVTIDFAGVDAKTKEAIAGADG
                     KDYPLVIGSNTFIPGFEPELVGLQAGDDKTFTVTFPKDYGAAELQNRQVSFTVHVSQV
                     QELTEPKLDDAFAASVGPFKTIAELKADIKKQLQADREQQSRQDYESAVLEALANKTK
                     VAIPDEIIDEEVQRAEAEERRNLTYRGTTWQEHLEQEGVSEEEHRAKQRPSAELRIRA
                     GLVLSEVAEREGIRVTPDEVDVRMQLLKGQYQDPAMQAELDKPEARRDIASRMLSEKT
                     IGKLTEYASAK"
     gene            1079..1474
                     /locus_tag="VFH39_04440"
     CDS             1079..1474
                     /locus_tag="VFH39_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925052.1"
                     /translation="MTHQAEFPYWVEFQDVDLRLPMGTLMVDGYPAGGYEQLATGRVI
                     MHTFLVNQGAGQQLLSHEPGRSERDIERLADQVLSKLVVCEDLEPRNGVVSIPAAREV
                     IEEACGLVPAVPYNLPRRRQDHRPSRHSI"
     gene            1542..>2068
                     /locus_tag="VFH39_04445"
     CDS             1542..>2068
                     /locus_tag="VFH39_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET6925053.1"
                     /translation="MSVLIPTVIENEGRYERAYDIYSRLLKDRIIFLGSDVNEASANT
                     IVAQLLFLQAQDAKKDIFLYINSPGGSVYDALAIYDTMQYVTNDVQTVGIGVQASAAA
                     FLLSSGTKGKRFLLPNSTVMIHQPSSGTRGKVTDQEIDLRESLRIKKLLEEIMAKNTG
                     QKISQIHEDMERDRW"
BASE COUNT          512 a          588 c          612 g          356 t
ORIGIN      
        1 aagtatccga ccggtaaatc agccccaagt gtctgtgacc aagttcgtgc cctacagcga
       61 tctggaggcc gactacgaag taaatgtcat cggcgatatc accatgccgg attacaagaa
      121 gttccgtctg ccgaagccga acgtcaaagt tgagaccaaa gatgtcgacg aagtcatgag
      181 taatctgcgc cagcgctccg cgaccaagca ggtagttgaa cgggccgcca aagacgggga
      241 cgaagtcact atcgatttcg cgggcgtcga tgccaagacc aaagaagcga ttgctggggc
      301 cgacggcaag gactacccgc tggtcatcgg cagtaacacg ttcatacccg gcttcgaacc
      361 ggaactggtc ggcctgcagg ccggtgatga caagaccttc accgtcacgt tccctaaaga
      421 ctacggtgct gccgagctgc agaaccgcca agtgagcttc acggtacacg tcagccaagt
      481 gcaggagctg accgaaccga agttggacga tgcgtttgcg gccagcgtgg gcccgttcaa
      541 gacgatcgcc gagcttaagg cggacatcaa gaagcagctg caggccgacc gcgaacagca
      601 gagccgccag gattacgaaa gcgccgtcct ggaagcgctg gccaacaaga ccaaggtcgc
      661 gataccggac gagattatag atgaagaagt acagcgcgcc gaggccgaag agcgccgtaa
      721 cctgacctac cgcggcacta cctggcagga acacctggag caggaaggtg tcagcgagga
      781 agaacaccgc gccaagcaac ggcccagcgc cgagctgcgc atccgggccg gtttggtgct
      841 gagcgaagtc gccgagcggg aaggcatccg ggtgacgcca gacgaagtcg atgtccgcat
      901 gcaactgcta aagggccagt accaggatcc ggccatgcag gccgagctcg acaagccgga
      961 agcccggcgc gacatcgcca gccgcatgtt gagcgagaag accatcggca aactgaccga
     1021 atacgccagc gcgaaatagg tcacattact tgtgctaatg gagcgtatag taacggctat
     1081 gactcaccag gctgaatttc cttactgggt cgaattccaa gatgtcgacc tgcgcctgcc
     1141 gatgggtacg ctgatggttg acggctatcc cgcaggcggc tatgagcagc tggcgactgg
     1201 ccgtgtcatc atgcatacct ttctggtcaa tcagggggcg ggccagcagt tgttaagcca
     1261 tgaaccgggc cggtcggagc gcgatatcga gcggctggcc gatcaggtac tgtcgaaact
     1321 ggttgtgtgt gaggacctgg agccgcgaaa cggggtcgtg agtatcccag ctgcccgcga
     1381 agtgatagag gaagcctgcg ggctggtccc ggctgtgccc tataacctgc cgcgtcggcg
     1441 gcaggatcac cgaccgtcca ggcacagcat ataattagca ctcttgactg ccgagtgcta
     1501 atttcatatc atagatatat acacagaaag caggtttatc tatgagtgta cttattccca
     1561 ccgttatcga gaacgaaggc cgctacgagc gggcgtacga catatattcg cggctgctta
     1621 aggaccggat catcttcctc ggcagcgatg tgaacgaagc cagcgccaac accatcgtgg
     1681 cccagctgct gttcctgcag gcgcaagacg ccaagaaaga catcttcctg tacataaaca
     1741 gccctggcgg cagcgtctac gacgccttgg ccatttacga caccatgcag tacgtgacca
     1801 acgacgtcca gaccgtcggt atcggcgtcc aagccagcgc agctgccttt ttgctgagca
     1861 gcggcaccaa aggtaagcgg ttcctgttgc cgaacagtac cgtcatgatt caccagccgt
     1921 ccagcggtac ccgcggtaag gtaaccgacc aggaaatcga cctgcgcgaa tccttgcgta
     1981 tcaagaagct gctggaagaa atcatggcca agaacaccgg ccagaaaatc agccagatcc
     2041 atgaggatat ggagcgtgac cgctggat
//
LOCUS       DASSFD010000184         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_898533, whole genome shotgun sequence.
ACCESSION   DASSFD010000184 DASSFD010000000
VERSION     DASSFD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898533"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..881)
                     /gene="atpB"
                     /locus_tag="VFH39_04450"
     CDS             complement(21..881)
                     /gene="atpB"
                     /locus_tag="VFH39_04450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET6925054.1"
                     /translation="MFTLLQHVADSSPAVHVAPATDFTLLGVHITNSILYGWLISLVM
                     ILLLVGVARKVRIHPRGGFVQYVEAGVDFCYNLVTGAFNDKAVGRKYVPYFVTLFFFV
                     LFHNWSELIPGVGEPLNVHGHPLFRPMTADLNATLAIGLVTMLVVYVASIKEVGGFRA
                     YIRHFFIGSPLNPLYLVIGLIEMLTDLTRTLSLALRLFLNITIGEIVISVFAFLGGML
                     APHSVLSPITALPFTLLELGVGALQAYIFVILGVNYLAISVNSAHAHDDLTEDEAPET
                     IGAAVEGAKG"
     gene            complement(881..1225)
                     /locus_tag="VFH39_04455"
     CDS             complement(881..1225)
                     /locus_tag="VFH39_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET6925055.1"
                     /translation="MIRSAAQQPTPPSFRGESTHKPARSQRADFLVMALNMSWQLAIA
                     VLVPVITGVYADKAAHTSNTFTFIGLALAFIGVGLVLWRTMQVANRLPVPKLTEAQKR
                     AIKKSYEAEDDD"
     gene            1310..1546
                     /locus_tag="VFH39_04460"
     CDS             1310..1546
                     /locus_tag="VFH39_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET6925056.1"
                     /translation="MNNSVIVYSATWCGFCHAAKQYLDKLGIKYEDRDVESKPEYMAE
                     AVTKSGQMGIPVLDINGEIIVGFDRPKIDAALLK"
     gene            1646..>2051
                     /locus_tag="VFH39_04465"
     CDS             1646..>2051
                     /locus_tag="VFH39_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925057.1"
                     /translation="MSEAFAHEPDRRLVGGHLSLIVDAENDNDVVIGFHGEILRQSTP
                     QSTVTERFAEFSRRNDINPQDVTPDTRHAFATTMHDFVFDDVTQHIRNKIGPEAPIIG
                     VLPRELVEKRLSLFYEQRDPADSDTVRRGLAER"
BASE COUNT          497 a          581 c          567 g          406 t
ORIGIN      
        1 tatccgacca cgccggagct tcatccctta gcaccctcga cagcagctcc tattgtttca
       61 ggtgcttcat cttcggtcaa gtcgtcgtgc gcatgggcac tgttgaccga aatggcgaga
      121 taattcacgc ccaggatgac gaaaatgtag gcctgcaagg caccgacgcc cagctccagc
      181 agggtgaagg gcagggccgt gatcggcgaa agcacgctgt gcggcgccag cataccaccc
      241 aagaaagcga agaccgagat gacgatttcg ccgatagtga tgttcaggaa caaccgcagt
      301 gccaagctga gcgtccgggt caggtcggtc aacatctcga tgaggccgat aaccaggtac
      361 agcggattga gcgggctacc gatgaagaaa tgccggatgt acgcccggaa accgcctacc
      421 tcttttatgg aggccacgta cacgacgagc atcgtcacca gcccgatggc cagggtggca
      481 ttcaaatcgg ccgtcatagg gcgaaacagc gggtggccgt gcacattcag cggctcgccg
      541 acgcccggta tcagctccga ccagttgtgg aacagcacga agaagaacag cgtcacgaag
      601 tacggtacgt acttgcggcc gaccgctttg tcgttgaacg cgcccgtcac caggttgtag
      661 cagaaatcga cgccggcctc cacgtactgc acgaaaccgc cgcgcgggtg gatccgtacc
      721 ttacgcgcca ccccgactag cagcagtatc atgaccagac ttataagcca accgtacaat
      781 atggagttag ttatgtgcac cccgagcagt gtgaagtctg tcgccggcgc cacatggacg
      841 gccggactgc tgtcggcaac atgctgcaaa agcgtaaaca tcagtcgtca tcctccgctt
      901 cgtaactctt tttgatggcg cgcttctgcg cctcggtcag cttaggcacg ggcaagcggt
      961 tcgccacctg catagtgcgc cacagcacca gcccgacccc gatgaacgca agcgccaagc
     1021 cgatgaacgt aaaggtgttt gaagtgtgag ccgccttgtc ggcatagact ccggtgatga
     1081 cgggtaccag tacggcgatg gccagctgcc agctcatatt cagggccata accaaaaaat
     1141 cagcccgctg gctgcgtgcg ggtttgtgcg tgctctcacc cctgaaagat ggcggcgtag
     1201 gttgctgcgc tgcggatcga atcatctttg tcttactata acagttatgc cagagctggg
     1261 caagtaaaca tactttacaa agtatagtaa ggcacttata ctggtaatca tgaataattc
     1321 cgtcatcgtt tacagcgcaa cctggtgtgg cttctgccac gccgccaaac agtacctcga
     1381 caagctcggc atcaagtacg aagatcgcga cgtcgagagc aagcccgagt acatggccga
     1441 ggccgtcacc aagtcaggcc agatgggtat tccggtgctc gacatcaacg gcgagataat
     1501 cgtcggcttc gaccggccga aaatcgacgc agcgctgctg aagtagcctc cgtagatgta
     1561 atcgacatca caaaatatgg gtagcatatc ttacatggga ctgacgtcat cttttctata
     1621 ctgacatcag taatagggga tagtcatgag tgaggcattt gctcacgaac ctgatagaag
     1681 gctggttggc ggtcacttga gcttaatcgt cgatgctgag aatgacaatg acgtggtgat
     1741 cgggttccat ggagaaatcc tacgtcagtc cacaccgcag agcaccgtca ccgaacgatt
     1801 cgctgaattc agtcgacgca acgacatcaa tccacaggac gtgacgcctg ataccaggca
     1861 tgccttcgcg actaccatgc acgatttcgt attcgatgac gtgacgcagc acatcagaaa
     1921 caagatcggc cccgaggcgc cgattatcgg cgtattgcca cgtgagctgg ttgaaaaaag
     1981 gctttccctc ttttatgagc agcgcgatcc ggccgactcc gataccgtgc gtcgcggcct
     2041 agccgagcgc g
//
LOCUS       DASSFD010000185         7413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1471907, whole genome shotgun sequence.
ACCESSION   DASSFD010000185 DASSFD010000000
VERSION     DASSFD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471907"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFH39_04470"
     CDS             <1..313
                     /locus_tag="VFH39_04470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6925058.1"
                     /translation="LAATAQAGNLFVAVEDERIVGTVMILDNPHTFWLLRFAVDPESA
                     QATDAAKLLFDKMSAVAAQRGHHSVIVYTDSDDEQLNRRYEDLKCHKAGNYRCYWKEV
                     R"
     gene            313..3270
                     /gene="ileS"
                     /locus_tag="VFH39_04475"
     CDS             313..3270
                     /gene="ileS"
                     /locus_tag="VFH39_04475"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET6925059.1"
                     /translation="MKFQAGQRRKAIEYEKDLVKYWKEHKTFEKSVEQRPVENSYVFY
                     DGPPFITGVPHHGTLLSSIVKDAVPRYWAMHGKRVERVWGWDCHGLPAEVFTEKKLGI
                     QDRRDIGTKISLEEYILTCRENMVQTGNLWEDTIDRIGRWVDFRGAYRTMDKDYMESV
                     WWAFKQLFEAGKIYEGEKVLLYCTRDATPLSKAEVTMDAGSYQDVNDPSVFVKFKLTD
                     EDAYLLAWTTTPWTLPANTAVAVNKDEDYSQVQLEDGTNVILATELLEKVLTDEKHNV
                     VPFTFVKAWKGSELVGKSYEPLFADRGINAHKVWHADYVSMEEGTGIVHLAPAYGEED
                     YALSKENNFPFVSIIDENGFYTEGDWKGQNVWEANKPIAKELHARGVVWKIEYVIHSY
                     PHCYRCGTRLMYRAHPSWFMDIQGQKQLMLEQNENVNWFPHHLKEGRFKKNIEQAPDW
                     NLSRDRFWATAMPVWKSDDGDVKVVGSYAELKELSGVELEDYHRPWIDDVEFDYQGKH
                     YKRIDKVMDCWFESGSMPFAQFHYPFENKERFESSFPGDFIVEYIGQVRAWFYYVHAV
                     NVGLHGTNAYKQVITTGTVLGNDGRKLSKSLGNYTDPNEVMDLYSADALRFTLLNSVL
                     LKGEDVTLKDKDVQDVQRKLAMIWNMYDFFTTYAAVDKWEWQGSHDDPSKELTNPLDV
                     WVVSRLHQLQAHLEQNMDAYDIPTALEAVLPFLDDASNWYVRRSRRRFWKSGDDADKQ
                     AAYTTLHYVLLRLSLILAPFIPFLAEELFLKLTGGEMGESVHLLDWPVGGVVDEQAVG
                     DMAKVREAINQGLSLRAAAGIKVRQPLAKVTLFGLPDLSSELTDIVKEELNVKAVELV
                     PRGPEVKPEQTFAEVREAKQGTYDLLDIRVELDTVITEDLRREGMMREIVRHVQQSRK
                     DAGLQVDDRIELHLFTADDELKAAITEHAETIVAETLAVSLSQADDSVTDAYAATVKV
                     EGAELAVALKKVS"
     gene            3682..3960
                     /locus_tag="VFH39_04480"
     CDS             3682..3960
                     /locus_tag="VFH39_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925060.1"
                     /translation="MFHHWQDVVLAICILGFNVALVPSVFSKSKPAVATRAITASALA
                     LSVIVYISLSLWYTVAMQALNCGLWVILGVQGYGAASQRTRTAGRRPR"
     gene            4039..4347
                     /gene="rplU"
                     /locus_tag="VFH39_04485"
     CDS             4039..4347
                     /gene="rplU"
                     /locus_tag="VFH39_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6925061.1"
                     /translation="MKKAVIATGGKQYLVSEGETLSVELLKGEKGKVAFDALLVIDGD
                     KVNVGSPLVDKVKVNADIVAEDVQADKVTSIRYKAKKRVKTVRGHRQHQTTLKITTIA
                     "
     gene            5107..>7413
                     /locus_tag="VFH39_04490"
     CDS             5107..>7413
                     /locus_tag="VFH39_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925062.1"
                     /translation="MRVRSGCVFAASLVLLLAAVIGMTQVRTDAATNGTLNFQAKLMT
                     SSGSIAADGNYNVEFNLYSQSSGGTSLWTEDHLNSAGTQLRVVNGYLTVNLGSVNPFP
                     STINWDQDLWLGMTVRGTGSCAFSACTPADAEMTPRLKLTATPYAFSAGKLSQYNSST
                     LFTSTLQLQAPTGGNQIFQVQDQGAAGTYNLLTQNQANGSYIQNQTGTAQQANFYIQS
                     ASAGSVGAIIQGATGQTADLFEAKDSTGNIRFNIFSGSGVGINTNGSRLNNAVVSVAG
                     NGTTTPEIALRAAGGQTVDILTALDSANDVLSGINFGGGFYSNGATNTFNALQPPAFT
                     TASTGTAYYYEIAATNASGETMPSSSLGMANNTSTLQWSQVPGATGYKIYRNTTNSFG
                     SGSLLLTTITNGATTSYTDTGSATGTGLPRTSPSTGNPLTVNGYTGFGLSVNSYGNTS
                     VTNSLSVSGLGGCYGVNTTLCVKSSATNAGQIVQGSTGQTADLVQYQGSNGVTMSGVD
                     ANGQFYGYGTNLTINNLAVPTLSVTTAGTTYYYVVTAVNNNGVETTPSNEVGATNSTT
                     ITWPSVAGATHYYVYRGTTPGGENLYFNNTSCSSGTCSFSTATSFSGSRTPPTTTTGS
                     GLTIQGWANQTGTLFNFTNSAGASVYRITAVGDFISNSMNNTVSGLIVGGGYTTHSTN
                     KLQVQTNGAVIGQVIQAGASQSADLLELQDSNGNPTTSFDKNGYIHINRATASTDTIF
                     TGRVSGDTNDRVQIQANGQINFGPGNAAT"
BASE COUNT         1697 a         2154 c         2186 g         1376 t
ORIGIN      
        1 gctggcggcg accgcgcagg caggcaactt attcgtggcg gtggaggacg agcggatagt
       61 tggaacggtc atgatcctcg acaacccgca cacgttctgg ctgttgcgct tcgccgttga
      121 cccggaatcg gctcaggcta ccgacgcggc aaagctgttg ttcgacaaaa tgagtgccgt
      181 ggcggcgcag cgcggtcacc acagcgtcat cgtctacacc gatagcgacg acgagcagct
      241 gaatcgccgt tatgaagatt tgaaatgcca taaagcaggg aattaccgat gttattggaa
      301 ggaggtgagg taatgaaatt tcaggctgga caacgccgca aggctattga gtacgagaaa
      361 gacctcgtca agtactggaa ggaacacaaa acgttcgaga agtcggtcga gcagcggccg
      421 gtcgaaaaca gttacgtgtt ctacgacgga ccgccgttca tcaccggtgt gccgcaccac
      481 ggcacgctgc tctcgagcat cgtgaaggac gccgtgccgc gctactgggc catgcacggc
      541 aagcgggtgg agcgcgtgtg gggctgggac tgtcatgggc tgcccgctga ggtgttcacc
      601 gaaaagaagc tgggcataca ggaccggcgc gacatcggta ccaagatttc ccttgaggag
      661 tacattctga cctgccgcga gaacatggtg cagactggta acctgtggga agacaccatc
      721 gaccgcatcg gccgctgggt ggacttccgt ggcgcctacc gcaccatgga caaagactac
      781 atggaaagcg tctggtgggc cttcaagcag ctgtttgagg ccggcaagat ctacgagggc
      841 gagaaggtgc tgctgtactg cacgcgcgac gccacgcccc tcagcaaggc cgaggtgacc
      901 atggacgccg gcagctacca ggatgtgaac gatccgagcg tgttcgtgaa gttcaagctg
      961 accgacgaag atgcctacct gctggcctgg accaccacgc cctggaccct cccggccaac
     1021 acggcggttg ctgtaaataa ggacgaagac tacagtcagg tgcagctgga agacggcacg
     1081 aatgtaattc tcgcaacaga gctgttggag aaagttctca ctgatgagaa gcacaacgtt
     1141 gtacctttca cctttgtaaa ggcctggaaa ggcagtgagc tggtcggcaa gagctacgag
     1201 ccgctgttcg cggatcgcgg tatcaacgcc cacaaggtct ggcacgccga ctacgtcagc
     1261 atggaagagg gtaccggcat cgtccacctg gcgccggcct acggtgaaga agactatgca
     1321 ctcagcaaag agaacaactt cccgttcgtc agcatcatcg acgagaacgg cttttacacc
     1381 gagggcgact ggaaaggtca gaacgtctgg gaggccaaca agccgatcgc caaggagctc
     1441 cacgcccgcg gcgtcgtctg gaaaatcgaa tacgtcattc acagctaccc gcactgctac
     1501 cgctgtggca cgcgcctgat gtaccgcgcc caccccagct ggttcatgga cattcagggc
     1561 cagaaacagc tcatgctgga gcagaacgag aacgtcaact ggttcccgca ccacctgaag
     1621 gagggccgct ttaagaagaa tatcgagcag gcaccggact ggaacctcag ccgtgaccgc
     1681 ttttgggcca ccgccatgcc ggtttggaag agcgacgacg gcgatgtgaa ggtcgtcggc
     1741 agctacgccg aactgaagga attaagcggc gtggagctgg aggattacca ccggccgtgg
     1801 atcgacgacg tcgagttcga ctaccagggc aagcactaca agcgtatcga caaggtgatg
     1861 gactgctggt tcgagagcgg tagcatgccg ttcgcccagt tccactaccc gttcgagaac
     1921 aaagagaggt tcgagagcag cttcccgggc gacttcatcg tggagtacat cggccaggtg
     1981 cgcgcctggt tctactacgt gcacgccgtg aacgtcggcc tgcacggcac caacgcctac
     2041 aaacaggtga ttacgaccgg tacggtgctc ggcaatgacg gccgcaagct cagtaagtcg
     2101 ctcggcaact acaccgaccc gaacgaggtc atggacctgt acagcgccga cgccttgcgc
     2161 ttcacgctgc tcaattccgt tttgctgaag ggcgaagatg tcaccctcaa agacaaagat
     2221 gtgcaggatg tgcagcgtaa gctggcgatg atatggaaca tgtatgactt ctttacaaca
     2281 tacgcggctg tcgataagtg ggagtggcag ggcagccacg acgacccgtc caaggagtta
     2341 accaacccac tggacgtgtg ggtggtgtca cgcttacatc agctgcaggc gcacctggaa
     2401 cagaacatgg acgcctatga catcccgacg gccctggaag ccgtactgcc gtttttggac
     2461 gatgccagca actggtacgt gcgccgcagc cgccgccgct tctggaagag cggcgacgac
     2521 gccgacaagc aggccgccta cacaacgctg cactacgtgc tgttgcgtct cagcctcatc
     2581 ctagcgccgt tcatcccgtt ccttgccgaa gagctgttcc tgaagctcac cggtggtgag
     2641 atgggcgaga gcgtccacct gctggactgg ccagtgggcg gggtcgtcga tgagcaggcc
     2701 gtcggcgaca tggccaaggt ccgcgaagcc atcaaccagg ggttgagcct gcgggccgcc
     2761 gctggcataa aggtgcgcca gccgctggcc aaggtgacgc tgttcggcct gcctgacctc
     2821 agtagcgagc tgaccgacat cgtcaaagag gagctgaacg tcaaagccgt cgagctggta
     2881 ccgcgcggac ccgaagtcaa gccggagcag accttcgccg aagtccggga agccaagcag
     2941 ggtacctacg acctgctcga catccgggtc gagctcgaca cggtcatcac cgaagattta
     3001 cggcgcgagg gcatgatgcg ggagattgtc cggcacgtgc agcagtcgcg caaggacgcc
     3061 gggctgcagg tggacgaccg gatcgagctg catctgttca cggccgatga cgagctgaag
     3121 gcggccatca cggagcatgc cgaaaccatc gtcgccgaga cgctggcggt cagcctgagc
     3181 caggccgacg atagcgtcac ggatgcttat gccgctaccg tgaaggtgga gggggcggag
     3241 ttggcggttg ccctcaagaa ggtgtcgtaa gggcagtatt cgatactgcc caactgggga
     3301 ggcctgtcct agggccgcta cgtgtgtcac acgggccgtt ttgaaggggt agggtacgtc
     3361 tcggaccgcc gtatcaactt gtgagccgct tcgcagcggc tcgtcttact tttgccttga
     3421 tgcaaaagta agcaaaagat caaggcccga ccgttccgcc tcggtcagct gttgtcacga
     3481 ctgacaaccg gctcgagaac tcctcgctcc gctcgtcaga cagctcgagc cgccctgcgg
     3541 gaagttgtca tcatgacaaa cagactgagc cggggctgca cttcagggcc atggctgctc
     3601 tgggcgggtg cttcagggcc attcctgcgc cgggttcgct cctagggccc ccttagctgc
     3661 cggggtggta tgatggtgct tatgtttcat cattggcaag atgtcgtcct ggcgatctgt
     3721 attctaggtt tcaatgtcgc gctcgtaccg agtgtgttca gcaagtcgaa accggcggta
     3781 gcgacccgtg ctatcacggc cagcgccctg gcgctttccg tcatcgtcta catcagtctg
     3841 tcgctgtggt acacggtggc catgcaggct ctcaactgcg gactgtgggt catcctcggt
     3901 gtccagggct acggcgcggc gtcacagcgt acccgcacgg caggacgccg cccgcgatga
     3961 gcgggagcgg cctttacagg gagcgccgta tctgttaaga tggttcggta tcaaaaattc
     4021 ctgagaggat ttcaagcaat gaaaaaagca gtcatagcca ccggtggcaa gcagtacctg
     4081 gtgagcgagg gcgagacgct gagcgtcgag ctgctgaagg gcgagaaggg caaagtggcc
     4141 ttcgacgcgc tgctggtcat cgacggtgac aaggtgaacg tcggttcgcc gctcgtcgat
     4201 aaggtgaagg tgaatgccga catcgtcgcc gaggacgtcc aggccgacaa ggtcaccagc
     4261 atccgctaca aggccaagaa gcgcgtgaag accgtccgcg gtcaccgcca gcaccagacg
     4321 acgctcaaga tcactacgat cgcctaaaca gcctcaaagc taccaagcct gaaaccgttc
     4381 cgaaaggggc ggttttttgg taaagcgggt acgtttgcgt gtcggcgtcc aagatgcttg
     4441 tggataaatt cggaggacaa cgaggcggtg tgtagttatt tacaactata ctttgctcaa
     4501 aacgaccctt caccatatct gaacaaggta attatcactt tacaatcggc tttgtgttta
     4561 aaaaaacaac tgtttttgat ggtgtaacac gtgtttttta ggcctaaaaa cgtatagatt
     4621 ttttgttcaa aaaagccttg aaacacctct cagcccgtcg gataatcata accagacagc
     4681 aacgctctaa aaaaatcagt tgctgcccca aactaaaact tcaaaccaaa aacctaaatg
     4741 cccacacaaa cacccaaaaa agggaaactg tttgcaagca ccctaaggag acacttccga
     4801 agggtgcttg tgggcgtctg ggcgggtttc ggcttgcggc acattgcggc ccgatcgctg
     4861 cgttccggtg ctcacgtacc acagaggtac gctccgctcc ggtcctcgcg ctcgaaccac
     4921 actgcaccac aatccgcacc ccgctatctc cgcgccaaac accgttatag gcaacggggg
     4981 cggtatagac cgttcgaggg catgttgaac ctgctcgcta ttgctcgccg tggtgcgggt
     5041 tttgtctttg ccgccttagg cggtgttttt tatacgcgcg cgtacgtata cgcgcgtggg
     5101 cgcgtagtgc gcgtgcgtag cggctgcgtg ttcgccgcca gcctggtgct gctgctggca
     5161 gccgtgatcg gcatgacgca ggtccggacc gacgccgcca ccaacggcac gctgaacttc
     5221 caggccaagc tgatgaccag cagcggctcc atcgccgccg acgggaacta caacgtcgag
     5281 ttcaacctgt acagtcagtc cagcggcggt accagcctct ggaccgagga tcacctcaac
     5341 agcgccggca cgcaactgcg tgtcgtcaac ggctacctga ccgtcaacct gggcagcgtc
     5401 aatccgttcc cgagcaccat caactgggac caggacctgt ggctgggcat gacggtgcgg
     5461 ggcaccggca gctgtgcctt ctcggcctgt acgccggccg acgccgagat gacgccgcgc
     5521 ctgaagctga cggccacgcc gtacgccttc agcgccggca aactgagcca gtacaacagc
     5581 agcacgctct tcaccagcac gctgcagctg caggccccga ccggcggcaa tcagatattc
     5641 caagtccagg accagggtgc tgccggcacg tacaacctgc tgacccagaa ccaggcgaac
     5701 ggcagttata tccaaaacca gacagggacg gcacaacagg cgaacttcta catccagagc
     5761 gccagcgccg gctcggtggg cgctattatt cagggtgcta ctggccagac ggccgacttg
     5821 tttgaggcta aggactcaac tggcaacatc cgttttaata ttttttccgg ctccggagtg
     5881 ggcataaaca ccaatgggag cagactcaac aatgcggtgg tttctgttgc tggcaacggg
     5941 acaacaactc ccgagatagc gctacgagct gcaggcggac agaccgtgga cattcttact
     6001 gcgctcgatt ctgcgaatga tgtactgtcg ggaataaact tcggcggtgg tttttacagc
     6061 aacggcgcaa ctaatacctt caacgccttg cagccacccg ccttcactac cgccagcacc
     6121 ggcaccgcct actactacga gattgccgcc accaacgcca gtggagagac gatgccgagc
     6181 agttcgttgg gtatggccaa caacaccagc acactccagt ggagccaggt accgggcgcc
     6241 accggctaca agatttatcg caacaccaca aacagctttg gcagcggcag cctgttgctt
     6301 acaaccatca cgaacggtgc cacgaccagc tacaccgaca cggggagtgc taccggcacg
     6361 ggcttgcccc ggacgtcacc gagcaccggc aacccgctga cggtgaacgg ttacacgggc
     6421 ttcggtctga gtgtgaacag ctatggtaac acctcggtca ctaactcatt gagtgtcagc
     6481 gggctgggcg gctgctacgg cgttaatacc accctatgcg taaagagcag cgccacgaac
     6541 gccggccaga ttgtccaagg gtcgacgggg caaactgctg accttgtaca gtaccagggc
     6601 tcgaacggtg taacgatgtc gggggtggat gcgaacggcc agttctacgg atatggcaca
     6661 aacctcacga ttaacaatct tgcagttcct acgctctcag tcacgactgc cggcacgaca
     6721 tactactacg tcgtgacagc ggttaataac aacggcgtcg agaccacgcc gtccaacgag
     6781 gtcggcgcta ccaacagtac aactatcacg tggccgtccg tggcgggggc aacccattac
     6841 tacgtgtacc gtggtactac gccaggcggg gagaatctgt acttcaacaa cacgagctgc
     6901 agcagcggca cgtgtagctt tagtacggcg acaagcttta gcggtagccg gacaccgccc
     6961 actaccacta ctggaagcgg cctcaccatt cagggctggg ccaaccaaac aggtacgctg
     7021 tttaacttta cgaatagcgc cggggccagt gtctatcgaa tcaccgctgt tggtgatttc
     7081 atttctaatt ccatgaataa cactgtttcc ggacttattg taggtggcgg atacaccact
     7141 cacagtacaa ataaactgca ggtccagacg aatggagctg taatcggtca agtcatacaa
     7201 gctggcgctt ctcagtcggc cgatctgttg gagctccagg actcgaatgg caatcctacc
     7261 actagcttcg acaagaacgg ctatatccac atcaaccggg cgactgccag caccgacacc
     7321 atctttaccg gtcgggtgag tggcgacacg aacgaccggg tgcagatcca ggccaacgga
     7381 caaattaact tcggcccagg gaacgcagca acg
//
LOCUS       DASSFD010000186         8644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1095106, whole genome shotgun sequence.
ACCESSION   DASSFD010000186 DASSFD010000000
VERSION     DASSFD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095106"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1023)
                     /locus_tag="VFH39_04495"
     CDS             complement(106..1023)
                     /locus_tag="VFH39_04495"
                     /inference="COORDINATES: protein motif:HMM:NF017919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HET6925063.1"
                     /translation="MALFPKRVDIDEASIRELVDMYDHAARYVRNTIFEVGRRKVTVV
                     SVQQARALIAQRLTQLTQESSLWAERQMTTLYNQGQQDAVDELRSFHDPRTDAVAAAI
                     VASGLRLHALHQEAAQALVSELQTRLRDAAATMDRSASKTLGQLLRESVTARMNEEAG
                     VATRQQLRRSVLQAFDDNGVSALYDAAGKRWSPSVYADMVSRTLSAQARNGGMMNSLK
                     SQGYDLVRVSAHGAGDACREWEDSILSMTGATPGYFTVSDATGAGLFHVNCQHSLDPV
                     DPADYPPDYLPDNLTEDQFQVLADNAIDE"
     gene            complement(1027..2652)
                     /locus_tag="VFH39_04500"
     CDS             complement(1027..2652)
                     /locus_tag="VFH39_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET6925064.1"
                     /translation="MAAQQQPAKSGSNNPNVFPYTNARPRLTRYNFNKQLFLGDHFDA
                     FNIKIDNRDFNKTYAKLRYVVNNFAGLLSKVSADFLFGEPPVIRLPLIDSANPYNAQN
                     DDGINPDANVDADADVFALKAPKPNPNQEWLENLIFENRLDVQFYESAITNSYKGDAI
                     FKLRVGPRRPDGPSEVIIEQVMPDYYFPDLDGSNVSAEPQRIEIAFITEIADKKYLRK
                     EIHTPGQIENQLFLMDGDKIVKPEPLELLGDPELQPIEQTGIDRILVVHVPNWSDGTA
                     HFGLDDYQDITTLIFALNNRLSLIDNILDKHADPILAVPEGVLDENGNIDRSVLGVYE
                     KSQDDSKPEYIVWDAKLDASFSEIEKLLEALYMYSETSPSVFGNTSGQTARTESGRAL
                     KYRLMRTVAKIQRKQRYYDYAIKEIVYLAELLAQAHGLLANGVPFKGQPEVPSIEWPD
                     PIPLDSYEQAQEEELRLASGNQSLRDSIKNVDKLDDEQAVQKMQEIFAEQKQLNPAPL
                     SVPTGTVPQTPTKTDPFAKNVQTPVTNRPNPPQ"
     gene            complement(2719..4284)
                     /locus_tag="VFH39_04505"
     CDS             complement(2719..4284)
                     /locus_tag="VFH39_04505"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925065.1"
                     /translation="MALPSPSELIAQVGEPQAKLIYREYFRDPANLEEFAELFYDYVP
                     TKLAEFHGEIFDYYEDEGNVGIAAPRGFSKSTITDTIYLAHRALYARSHFPVLISDTY
                     TQATMLLDGLGSELGGSNELIEWIYGDVKGERWSSEGLIVRGYAEDGSREEVLIIPRG
                     AGMKVRGLRFKQYRPDLIIMDDLENDELVESPERREKLYNWLVKAVIPAMAKDVGKII
                     IIGTTLHRESLLSRILRREGIFSGWKTHRYQGITDGKSLWPERFSIEHLLAMRDDPQH
                     EMYLGPISFSQEIQNEPISEQDQIIRPEWLQSRYSLLDTVTAYQHVIRARTYEKALYE
                     WLSRTFSNVVGHIDPAISEKETADWWAMLTIGVTKACPLCEGHPAGHVMILDYLRFRE
                     ADPEKQANMIGEQFLTWRHDKLKLEAVAYQAGLYQLTRRIAQSKHVNLPIVPWKPDRS
                     KRRRAIMQAGMFAGGMVHLRSDHPLCGPFVEEVVQFPQGDHDDMFDAYLGAAEQLVLK
                     SKRRVFTNKPKGF"
     gene            complement(4265..4756)
                     /locus_tag="VFH39_04510"
     CDS             complement(4265..4756)
                     /locus_tag="VFH39_04510"
                     /inference="COORDINATES: protein motif:HMM:NF027987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HET6925066.1"
                     /translation="MAAPRNDKDFQQKHVGRPLKFKTAKELQAKIDDYFDWCDNRIRT
                     TIDRDGNEKTYNFAAPYTMAGLAYFLDIERQTLVNYSHREPFFDTVARARARIAMDLE
                     TRIIESRTPQGGMFLAKNNFAYVDESRQTIDGNLKTSSELTPEAQAILAKASRAKDGP
                     TKS"
     gene            complement(4734..4982)
                     /locus_tag="VFH39_04515"
     CDS             complement(4734..4982)
                     /locus_tag="VFH39_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925067.1"
                     /translation="MGVVTKKYGKCESCPNQADAMRYSNGIEVWKDENGDWKYANYLI
                     KHPMCGYHQHQYALWMEAEKKKREKEAKQKRHGSTTKR"
     gene            complement(5210..5512)
                     /locus_tag="VFH39_04520"
     CDS             complement(5210..5512)
                     /locus_tag="VFH39_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925068.1"
                     /translation="MMRPTGDLLEAIASDPNRLLEAEGKKIGRLYTDHGLPLDMAFDR
                     IDIPKQQKLVVLHGALAWLIEHKRNSGATDKAIERQRKANREALDRYVTTGEIGMY"
     gene            complement(5509..5676)
                     /locus_tag="VFH39_04525"
     CDS             complement(5509..5676)
                     /locus_tag="VFH39_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925069.1"
                     /translation="MIHFVISVATYVAAHVLGLSATQPAAPVQYYQPAMQTIQLQAAN
                     EPLQGSNVELQ"
     gene            complement(5715..5870)
                     /locus_tag="VFH39_04530"
     CDS             complement(5715..5870)
                     /locus_tag="VFH39_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925070.1"
                     /translation="MQEEQRTIVYIRRLVGELERLMQMEQSGELSPAESEVLRNLCIN
                     LLQMYYK"
     gene            complement(5874..6665)
                     /locus_tag="VFH39_04535"
     CDS             complement(5874..6665)
                     /locus_tag="VFH39_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET6925071.1"
                     /translation="MGGQPKQGAPRLCTYDIESSFNIGGYFGRPYDANISKVIQRFYV
                     FGFAYKYMDERTVRTCYIWDFPLYKQDPRNDIEVVKKWQEVMAGVDYRIGHNSNSFDN
                     QIMRGRVAIHRLPLQSEPENIDTLRMAKRVGRYDSYKLDDLGEMFGLGRKLHTDADLW
                     WDCMQGVEKQMKRMVTYNKRDVALTEKLYKFLMQYDPTAGGHRNRANIAERPGACPYC
                     GVSGFMQSAGWKYTSISRYRQFMCGNCHRKSRSRTADKTDKPEYV"
     gene            complement(6670..7155)
                     /locus_tag="VFH39_04540"
     CDS             complement(6670..7155)
                     /locus_tag="VFH39_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925072.1"
                     /translation="MQPTLPVTIRKSFIGLFAIYLVGFLLTVALLGAGLYLLLRTDSR
                     PLGVVLLAVAAFTVLGTLVQGYVYSLCRIVLTEAELDVINWSSLFSQNNAVCEWRMVN
                     DVDFKKAGILSFLFDYGTLLVQTAGTERNLRISYVPRVEKVRDLIAQLADTAITPVRS
                     E"
     gene            complement(7174..7371)
                     /locus_tag="VFH39_04545"
     CDS             complement(7174..7371)
                     /locus_tag="VFH39_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925073.1"
                     /translation="MPAEFWDALEESSGRKTWPMHLVAVSYLSWKLMGRIDYQKLLDE
                     DAATKRVGVGKIKFMIDRPKH"
     gene            complement(7374..7820)
                     /locus_tag="VFH39_04550"
     CDS             complement(7374..7820)
                     /locus_tag="VFH39_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925074.1"
                     /translation="MRSVGTEKELRRQIARYLQAFHPEVVYRFDLAADMKLTKGQAAR
                     HKELHPHRGYPDLFLAHSTLQDGTVWPCAGLYLELKREGESPYLKDGKTLKADPHVRE
                     QAAMLDRLRDQGYYAEFAVGFEEAVGYIEAYLMPSDIAAMQESTVF"
     gene            complement(7813..8007)
                     /locus_tag="VFH39_04555"
     CDS             complement(7813..8007)
                     /locus_tag="VFH39_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925075.1"
                     /translation="MTELEYYLVKTALTLGVRPATISRDMELSMTAVTIAQGCDTYEQ
                     FEQMASPEGYAELQRALFNA"
     gene            complement(8007..8138)
                     /locus_tag="VFH39_04560"
     CDS             complement(8007..8138)
                     /locus_tag="VFH39_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925076.1"
                     /translation="MGTQGLILTLDGYGYGYGDGYGYGYGYGYGNGDGYGNGDGRGW"
     gene            complement(8120..8410)
                     /locus_tag="VFH39_04565"
     CDS             complement(8120..8410)
                     /locus_tag="VFH39_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925077.1"
                     /translation="MAKAKTVTRTKAHPTQIVVADRGFVYVGQVALEGDWVIVREARN
                     IRYWGTTRGLGQLALEGPTASTKLDPVGTVRIPARAVISLIDTEAALWARKG"
BASE COUNT         1414 a         2433 c         2788 g         2009 t
ORIGIN      
        1 agttggtgct gtaggcgcat ctgccatata atcccttctc ggtttttagc gtcgtccgtt
       61 cgacgtgtta gtggatttac agctaaataa tctcagttat gcttactatt cgtcaatagc
      121 gttatctgct aacacctgga attggtcttc ggttaggttg tcaggcaaat agtcgggcgg
      181 gtaatcggcc gggtctactg ggtctagcga gtgctgacaa ttaacgtgga aaagaccggc
      241 tcccgtggca tctgacacgg tgaagtagcc aggcgttgcg ccagtcatgc tcaagatgga
      301 gtcttcccac tcgcggcagg catcgccggc accgtgggcg ctgacccgca ccaggtcgta
      361 accctggctc ttgaggctgt tcatcatgcc gccgttgcgg gcctgggccg ataaggtgcg
      421 gctcaccatg tcggcgtaga cgctcggact ccagcgcttg ccggcggcgt cgtagagggc
      481 ggagacgccg ttatcgtcga aggcttgcag caccgagcgg cgcagctgtt gccgggtggc
      541 tacgccggct tcttcattca tgcgggcagt gaccgactca cgcagcagtt ggccgagcgt
      601 cttgctggcc gacctgtcca tggtggcggc ggcgtcacgc aaccgggtct gtagctcgct
      661 cacaagggct tgcgcggctt cctggtgcag cgcatgcagt cgcaggccac tggccacgat
      721 ggcggcggct acagcgtccg tacgcgggtc gtggaagctg cgtagctcgt ccacggcgtc
      781 ctgctggccc tggttgtaga gggtggtcat ctgccgttcg gcccacaggc tcgattcttg
      841 ggtcagttgg gtgaggcgct gggcgatgag ggcacgggcc tgctgcacgc tcaccacggt
      901 caccttgcgg cggccgacct cgaagatggt gttacggacg tagcgggcgg cgtggtcgta
      961 catgtcgacc agctcgcgga tgctggcctc gtcgatgtct acgcgcttcg ggaacagcgc
     1021 catggcctac tgcggcgggt taggccggtt agttacgggt gtctgcacgt tcttggcgaa
     1081 ggggtcggtc ttggtcggcg tctgcggcac ggtgccggtt ggcacactca gcggtgcggg
     1141 gttgagctgc ttctgctcgg cgaatatctc ctgcatcttc tgcacggcct gctcgtcatc
     1201 cagcttgtcg acgttcttga tgctgtcgcg caggctctgg ttgccgctgg ccaggcgcag
     1261 ttcttcctct tgggcctgct cgtagctgtc gagcgggatg gggtcaggcc actcgatgct
     1321 cggcacttcc ggctgaccct tgaagggcac gccgttggcc agtaggccgt gggcctgggc
     1381 cagcagttcg gccaggtaga cgatttcctt gatggcgtag tcgtagtaac gctgcttgcg
     1441 ctgaatctta gcgacggtac gcatcaggcg gtacttcagt gcccggccgg attcggtgcg
     1501 ggccgtctgc ccgctggtgt taccgaagac gctggggctg gtttcggagt acatgtagag
     1561 cgcttcgagc agcttctcta tctcgctgaa gctggcgtcg agcttggcgt cccagacgat
     1621 gtactccggc ttggagtcgt cctggctctt ctcgtagaca cccagcaccg agcggtcgat
     1681 gttgccgttc tcgtcgagca cgccttcggg tacggccagg atggggtcgg catgcttgtc
     1741 gaggatgttg tcgatgaggc tgaggcggtt gttgagggcg aagatgagcg tcgtgatgtc
     1801 ctggtagtcg tcgaggccga agtgggccgt gccgtcgctc cagttgggga cgtggacgac
     1861 gaggatgcgg tcgatgccgg tctgctcgat gggttgcagc tcggggtcac cgagcagttc
     1921 cagcggttcc ggcttgacga tcttgtcgcc gtccatcagg aagagctggt tctctatctg
     1981 gcccggcgtg tgaatctctt tccgcaggta cttcttgtct gctatctcgg tgataaaggc
     2041 aatctcgatg cgctgcggct cggcgctgac gttgctgccg tcgaggtcag ggaagtagta
     2101 gtcgggcatg acctgctcga tgatgacctc gctcgggcca tcgggccggc gcgggccgac
     2161 ccgcagcttg aagatggcgt cgcccttgta gctgttggtg atggcgctct cgtagaactg
     2221 cacgtcgagg cggttctcga agatgaggtt ttccagccac tcctggttgg ggttcggctt
     2281 aggggccttg agggcgaaga cgtcggcatc ggcgtcaacg ttggcgtcgg ggttgatgcc
     2341 gtcgtcgttc tgggcgttgt aggggttggc gctgtcgatg agcggcagac ggatgaccgg
     2401 cggctcaccg aacaggaagt cggcgctgac cttgctcagc agaccggcga agttgttgac
     2461 gacgtagcgc agcttggcgt acgtcttgtt gaagtcgcgg ttgtctatct tgatgttgaa
     2521 ggcgtcgaag tggtcgccca ggaacagctg cttgttgaag ttgtagcgcg tcaggcgcgg
     2581 ccgggcgttc gtgtagggga agacgttggg gttgttactg cccgatttcg ctggttgttg
     2641 ctgtgctgcc atgtgtgttg ctgtttatgc tttttactgt atcatgtacg gcccgcaaaa
     2701 acgcaacaaa ataatcggtt agaagccttt tggtttgttg gtgaagacgc ggcgcttcga
     2761 tttgagcacc agctgctcgg ccgcgcccag gtaggcgtcg aacatatcgt cgtggtcgcc
     2821 ttgcgggaat tgcacgactt cctcgacgaa cggcccgcac agcggatggt cgctgcgtag
     2881 atgcaccatg ccgccggcga acatgccggc ctgcatgatg gctcgacggc gcttcgagcg
     2941 gtcaggcttc cacggcacga tgggtaagtt gacgtgcttg ctttgggcga tgcgccgggt
     3001 cagctggtac agaccggcct gataggccac ggcttccagc ttcagcttgt cgtgccgcca
     3061 ggtgaggaac tgctcgccga tcatgttggc ctgcttctcc gggtcggctt cgcggaagcg
     3121 caggtagtcg agaatcatga cgtgaccggc cgggtgacct tcgcacagcg ggcaggcttt
     3181 ggtgacgccg atggtcagca tggcccacca gtcggccgtc tccttctcgc tgatggccgg
     3241 gtcgatgtgg ccgacgacgt tggaaaaggt gcggctcagc cactcgtaga gggctttctc
     3301 gtacgtccta gcccggatga cgtgttgata ggccgtgacg gtatccagca ggctgtagcg
     3361 gctctggagc cactcaggcc ggataatctg gtcttgctcg ctgatgggtt cgttctggat
     3421 ctcctggctg aagctgatag gcccgaggta catctcgtgc tgcgggtcat cccgcatggc
     3481 aagcaggtgc tcgatgctga agcgctcggg ccacaggctc ttgccatcgg tgatgccctg
     3541 gtagcggtgc gtcttccagc cagagaagat gccctcacgc ctcaggatgc ggctgagtag
     3601 agattcacga tggagcgtcg tcccaataat tatgatcttg ccaacgtctt tagccatggc
     3661 cggaatgact gccttgacta gccagttgta cagcttctcg cgtcgctcag gactttcgac
     3721 cagctcgtcg ttctctaggt cgtccatgat gatgaggtcg ggcctgtact gcttgaagcg
     3781 cagaccccgc accttcatgc cagcgccgcg cgggatgatt agtacttcct cgcgtgaacc
     3841 gtcctctgcg taacctcgaa caatcagacc ttcgctgctc caacgctcac ccttgacatc
     3901 gccgtatatc cactcgatta gctcattgct tccgcctaat tcgctaccga ggccatccag
     3961 gagcattgtc gcttgggtat aggtatcgct gatgaggacc gggaagtgtg aacgggcata
     4021 caaggcgcga tgagcgaggt agatagtgtc ggtgattgtg ctcttggaga agccacgagg
     4081 cgcggctata ccgacgttgc cttcgtcttc gtaatagtca aagatttcgc cgtggaactc
     4141 agcgagcttg gtcggcacgt agtcgtagaa cagctcggcg aactcttcga ggttggccgg
     4201 gtcgcggaag tactcccggt agatgagctt ggcttgaggt tcgcctacct gagcaatcag
     4261 ctcgctagga cttggtaggg ccatccttgg ctcgggaagc cttggcaagt atcgcttgtg
     4321 cttcaggggt tagttcactg gatgttttga ggttgccgtc tatggtttgc ctgctctcat
     4381 cgacataggc aaagttgttc ttggcgagga acatgccacc ttgcggcgtg cggctctcga
     4441 tgatgcgcgt ctctaaatcc atcgctatgc gggcgcgggc gcgggctacc gtgtcaaaaa
     4501 acggctctcg atgcgagtag ttcacaagcg tctgccgctc aatatccaag aagtacgcaa
     4561 gccctgccat cgtgtacggc gcagcgaagt tgtaggtctt ctcgttgccg tcacgatcta
     4621 tagtcgtgcg gatgcggtta tcacaccagt cgaagtaatc atctatcttc gcctgtagtt
     4681 ccttggccgt cttgaacttc aacgggcggc ctacgtgctt ctgctgaaag tccttatcgt
     4741 ttcgtggtgc tgccatgacg cttctgctta gcttccttct cgcgcttctt cttctcagcc
     4801 tccatccaca gcgcgtattg gtgttggtga tagccgcaca tcgggtgctt gatgagatag
     4861 ttggcgtact tccagtcgcc gttctcgtct ttccacactt cgatgccgtt ggaataacgc
     4921 atggcgtcgg cctggttcgg gcagctctcg cacttgccgt acttcttggt gacgacaccc
     4981 atcagcggat accgctgact ttgatgatga tgtaggagcc gatgacggtc agtaccagtg
     5041 tgcaaattaa gtctgcggcc atatatgcac tactaaacgc tagtgcttcg ttttagtcaa
     5101 taactaccgg aataagcgca gcagcgtgaa cagcaggctc accttgctga cgcggatggt
     5161 tggcttgccg tagcgggtgc cgatgtagat tctcatttgg tgattctcct taatacatgc
     5221 caatctcgcc ggtcgtgacg tagcggtcga gggcttcgcg gttagctttg cgctggcgtt
     5281 ctatcgcctt gtcggtcgcc ccggagttgc gtttgtgctc gatgagccag gccagtgcgc
     5341 cgtgcagcac cacgagcttc tgctgcttgg gtatgtcgat gcggtcgaag gccatatcga
     5401 gcggcaggcc gtggtcggtg tagaggcggc ctatcttctt gccctcagcc tctagcagcc
     5461 ggttggggtc gctggcgatg gcttcgagca ggtcgccggt aggacgcatc actgcaactc
     5521 gacgttgctg ccttgcaacg gttcattagc ggcttgcagc tggatagtct gcatagccgg
     5581 ctggtaatac tgcacgggtg cggccggctg ggtggcgctg agtccgagga cgtgggccgc
     5641 gacgtaggtg gcgacactga taacgaaatg aatcatagct tggttcctta tttagttgtc
     5701 agcctcatta tctgctattt gtaatacatt tgcaagaggt ttatgcacag gttccgcagt
     5761 acctctgatt cagctgggga taactcgccg ctttgctcca tctgcatgag ccgttccagc
     5821 tccccgacga ggcgacgaat atagacgatg gtgcgctgtt cctcttgcat ggcttagacg
     5881 tattcgggct tgtcagtctt gtcggccgtg cggctgcggc tcttgcggtg gcagttgccg
     5941 cacatgaact ggcggtagcg gctgatgctg gtgtacttcc agccggctga ctgcatgaag
     6001 ccggagacac cgcagtaggg gcaggctccc ggccgttcgg cgatgttggc ccggttgcgg
     6061 tggccgccgg ccgtcgggtc gtactgcatg aggaacttgt acagcttctc ggtcagggcg
     6121 acatcgcgct tgttgtaggt gaccatgcgc ttcatctgct tctcgacgcc ctgcatgcag
     6181 tcccaccaca ggtcggcgtc ggtgtgcagc ttgcggccca ggccgaacat ctcgcccagg
     6241 tcgtcgagct tgtagctgtc gtagcggccg acccgcttgg ccatcctgag cgtgtcgatg
     6301 ttctccggct cgctttgcag cggcaggcgg tgaatcgcta cccggccgcg catgatttgg
     6361 ttgtcgaagc tgttggagtt gtggccgatg cggtagtcga cgccggccat gacctcctgc
     6421 cacttcttga cgacctcgat gtcgttgcga gggtcttgct tgtatagcgg gaagtcccag
     6481 atgtagcagg tacgcaccgt gcgctcatcc atgtacttgt aggcgaagcc gaagacgtag
     6541 aagcgctgga tgaccttgga gatattggcg tcgtagggcc ggccgaagta gccaccgata
     6601 ttgaaactgc tctcaatgtc gtaggtacag aggcggggcg caccttgttt cggctgccca
     6661 cccatgcccc tactcgctcc tgaccggcgt tatggcggta tcggccagct gggctatcag
     6721 gtcgcgtacc ttctcgaccc gagggacgta ggagatgcgg aggttgcgct cggtgccggc
     6781 ggtctgtacc agcagcgtgc cgtagtcgaa caggaagctc agtatcccgg ccttcttgaa
     6841 gtcgacgtcg ttgaccatgc gccattcgca gactgcgttg ttctggctga acaggctcga
     6901 ccagttgatg acgtccagct cggcctcggt gagcacgatg cggcagagtg agtaaacgta
     6961 tccttggacg agcgtgccca gcaccgtgaa ggcggccacg gccagcagca cgacaccgag
     7021 cgggcggctg tcggttctga gtagcaggta gaggccggcg cccagcaggg caacggtgag
     7081 taggaagccc acgaggtaga tagcaaacaa gccgataaac gatttcctaa tcgttaccgg
     7141 cagtgtaggt tgcattatgt ctaaagcata ttgctaatgc ttcggccggt caatcatgaa
     7201 tttgatcttt ccgacgccta cccgtttcgt tgccgcgtct tcatcgagta gcttctggta
     7261 gtcaatccgt cccatcagtt tccagctcag gtagcttacg gccacgaggt gcattggcca
     7321 cgtcttacgg cccgacgatt cctctagcgc gtcccagaat tcggctggca tggctagaag
     7381 accgtgcttt cttgcattgc tgctatatcg gacggcatca gatacgcctc gatgtagccg
     7441 acagcttcct cgaagccgac cgcgaactcg gcataatagc cttggtcgcg cagccggtcg
     7501 agcatggcgg cttgctccct gacgtgcggg tcagccttga gcgtcttgcc atccttcagg
     7561 tatggactct cgccctcgcg cttcagttcc aggtacaggc cggcgcaggg ccagacggtg
     7621 ccgtcttgca gggtgctgtg ggccaggaat aaatctgggt agccccggtg cgggtgcagc
     7681 tccttgtggc gggcggcttg gcccttggtg agcttcatgt cggcggccag gtcgaagcgg
     7741 tacaccacct cggggtggaa ggcttgcagg tagcgggcta tctgccggcg cagttccttc
     7801 tcggtgccga cgctacgcat tgaacagtgc cctctgtagc tcggcgtagc cttccgggga
     7861 agccatctgc tcgaactgtt cgtaggtgtc gcagccttgg gcgatggtca cggccgtcat
     7921 agacagttcc atgtcgcggc tgatcgtggc cggtcggacg ccgagcgtca gtgcggtctt
     7981 tacgaggtag tattcaagct cggtcatcac caccctcgtc cgtcgccgtt gccgtagccg
     8041 tcgccgttgc cgtagccgta gccgtagccg tagccgtagc cgtcgccgta gccgtagccg
     8101 tagccgtcga gcgtcaggat tagcccttgc gtgcccatag cgcggcctcc gtgtcgatca
     8161 gcgagatcac ggcgcgggcc gggatgcgca cggtgccgac gggatcgagc ttcgtactgg
     8221 cggtcggccc ctccagcgcg agctggccaa gtccgcgggt cgtgccccag tagcggatat
     8281 tccgcgcctc gcggacgatc acccagtcgc cctcaagggc gacctggccg acataaacaa
     8341 atccacggtc ggccacgacg atctgcgtcg ggtgggcctt ggtcctggtc acagtcttcg
     8401 ccttcgccat cgatcagtcc tcgcggccct gtcaggttgt gcgccgcccg gccgggccga
     8461 gcccgggcgg cacagaggtc cgtcgtgggt gggggtgctt ctcaagccgg aaaactaatg
     8521 gccgtccgtg gccgactccg cgccgtcacc gcgggggtcc gtcatgggtc ggatggcttg
     8581 agggtttgcg ctagctcttc aggggtcagg aactcgacgc cgagtcgccg cgcggccgcc
     8641 acct
//
LOCUS       DASSFD010000187         6388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1280023, whole genome shotgun sequence.
ACCESSION   DASSFD010000187 DASSFD010000000
VERSION     DASSFD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280023"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFH39_04570"
     CDS             complement(<1..641)
                     /locus_tag="VFH39_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925078.1"
                     /translation="MQRMDQVADELRERQPETVTRGEIDAYQGELNDLCPYIGEQCQI
                     AGNGQWPIFDSDGENVGEEWRYETGMIAESHGVAIERLDQDAYYRPYHKFYLGFAKRR
                     DRATKEAVFNNYALLDRMGSIVLKRTVDDVFSDDESRSIFIPADFDTNLQAHSDWFRS
                     NLTSRAFRRRTLTSQQQFVDNYLAFVTDKFKLERAGVSFVATYCLRAASLSVL"
     gene            complement(748..1539)
                     /locus_tag="VFH39_04575"
     CDS             complement(748..1539)
                     /locus_tag="VFH39_04575"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6925079.1"
                     /translation="MWAQFWHDRLKRPFKLHYEVYGNGPVTVLLLHGIASSGSFWQPL
                     IDELDHSRFTLIVPDLLGHGASPRPPYLTYSTADQAVSVLALLKRLKVRHCLVVGHSM
                     GCLVASRLTAMAPARVERLLLYEPPLFADLPEFKSHARRRKFYLDLYARIAANPAGRL
                     TMTRLVARISKKWTAFLASDQTWLPIERSLRNAIIAQESFEELKDIAVQTDIVHGRLD
                     VIVPKAQLKDMLSSNPNISFFRTTDRHGLSRRSARYLARLISEPA"
     gene            complement(1547..2107)
                     /locus_tag="VFH39_04580"
     CDS             complement(1547..2107)
                     /locus_tag="VFH39_04580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6925080.1"
                     /translation="MDDRLTVAVLAGTTREKRESIKAAHYIADFAARQPGVDVIFVDP
                     KEFNFPGDGNDPEGKDPRYTDIVAKADAFFVVTPEYNHSFPGSLKRMLDSELELYNHK
                     PVAFAGASNGNWGGVRAVESLLTAVRETGLVALSWDVYFPRVQDIFDEAGQMKPEYIT
                     RYEHNVQKLFDELLWFARLLKAARQT"
     gene            complement(2150..2530)
                     /locus_tag="VFH39_04585"
     CDS             complement(2150..2530)
                     /locus_tag="VFH39_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET6925081.1"
                     /translation="MLDISDDRFQELIDEALGELPREQTDGLKNVAILFEDEPTPEQR
                     VRLNLHMGESLYGLYEGIPLAFRQGNQPLLPDRITIFKIPMLMSSPDVPALKAQIKHT
                     LWHEIGHYYGLDHDRIHEIEAHWH"
     gene            complement(2575..4815)
                     /locus_tag="VFH39_04590"
     CDS             complement(2575..4815)
                     /locus_tag="VFH39_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET6925082.1"
                     /translation="MVARQRLLRVGTEQIQRGLFVLISLLFVFGAALQAAPGAAALSE
                     PNDEIHSSFGDNATEEWVYWHGPDNTLNYGLTANIYTQSATAVNPSVQPVDGTTGPFW
                     QVKLTGLTPGTTYHYQIGTSGFDHTFQTEPTGDFTWDDVGDTGSTWYESDAPASCNKT
                     WMPEVWQQLANDQPNFVTHGGDIDYENECGQPSVHRLWNDIETVSEQTSMQFVWGNHE
                     YGSPSADAPPGTPKDSMANYKGRFNIPNAQTISNDTATNVKAPGCPSATDPTVNGCRG
                     DDWGYFTAGHVLFIQMPEAWYNAYTDWEPAADQLMAQAEADPNIYFIVTMGHRPAYSS
                     STNQVSAAVTTVVNELGDKYSPSARPDGKYVLNVAHHVHGGEVFSPQHGVVNVTNGGG
                     GSEEMSLSSIAGTQWFTGHPEHMRTTVTGNSMTLNFICGPVFTLNPNKEPCTKDSVLY
                     SLTLHGYQADQTPATPVLATALDDGVSSASVGDALHYQLTVSDTQSGTTAAGVTPSLT
                     LPSNVTITDAAGGTVNGQTVTWPSADIVSGQPWTQTVTAQLASGAGGSSFSANATVQA
                     ADCQNSGSVCQVSDTDTVTTTPTTVEYITNPSVESSLTGWTGLYNSLSKANRITTDAY
                     DGSASIQLLRNTTTAGAAGIRAKPDPVASAVAGDTYTASAWLRSEVANQKLIIMIQEK
                     DANGNVVSSGSTAQTLSDTNWHQLSVSYTAQTSGDSLAFVVYNPNMPANDWFHMDLMS
                     MTGPGS"
     gene            complement(4929..5621)
                     /locus_tag="VFH39_04595"
     CDS             complement(4929..5621)
                     /locus_tag="VFH39_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925083.1"
                     /translation="MAAYFLSAGLNFLAVFGGMLLFFLPFCGLFAYRHRRQILAVRPH
                     FNYFMERFFGSPEFNALVFVWAAGEALVWYIIPEFLLFLVVFMKVHRKFDLVKYDLFG
                     TIAGTLIGLVWHLPAQAFLRLPFIRPAMMTQVHDWYASAGVWGLAHQPFSGVPYKVFT
                     HEAAGWHFYIPLFIGIAIVARMARYLVAYGATVAIYPLLHRFVRKHYAILFVVASVVF
                     TLLLTRVVQIYS"
     gene            complement(5603..>6388)
                     /locus_tag="VFH39_04600"
     CDS             complement(5603..>6388)
                     /locus_tag="VFH39_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET6925084.1"
                     /translation="AGAFAAGTNDSGRVFMVAAVVVLVHSIVTVWNDLVDEAGDHYND
                     IHRVAALRKQGLLTMVRAGLYILVAAVVVLLRFLPLTEVLLTLVLFGLGWLYNREPVR
                     ASRRPVLSMVILALSYGALPFLMGAGLGQPTRLVWLMLLAWMLSRASLSLLKDYKDAP
                     GDAQAGKRTFLLRYGGTLTARLSFGLAAAGLLSLTILTVYVTDTRQLSVAGSLCIVVV
                     GLMVLRARLFAAHDYASLNRIFHEAVQFQLVFDALVILWLRIS"
BASE COUNT         1279 a         1928 c         1882 g         1299 t
ORIGIN      
        1 tcaagaaccg acagggaggc cgcgcgcaga cagtacgtcg cgacaaaact cacccctgcc
       61 ctctcgagct tgaacttatc ggtgacgaac gccagataat tatcaacgaa ttgctgctgg
      121 ctagtgaggg tgcgccggcg aaaagcccgc gacgtcaggt tagatctgaa ccagtccgaa
      181 tgcgcctgca gattcgtgtc gaaatcagct ggaataaaga tagatcggct ttcgtcgtca
      241 ctaaaaacgt catccacagt ccgtttcaag acgatgctac ccatgcgatc gagcaacgca
      301 taattattaa ataccgcttc tttcgtcgct ctgtcccggc gcttggcaaa ccccagataa
      361 aacttgtggt acggacggta gtacgcgtct tggtcgagcc gttcgatagc cacgccgtgt
      421 gattcagcaa tcatgcccgt ttcgtagcgc cactcctcac cgacattttc accgtcggaa
      481 tcaaatatcg gccattgtcc attgccggca atctggcact gctcgccaat atacggacag
      541 aggtcgttta gctcaccctg gtacgcatca atctcgccac gcgtcaccgt ttcgggctga
      601 cgttcgcgca gttcgtcagc tacctgatcc atgcgttgca tatagtcagt tatgtgccgc
      661 tccgtatcct gtgcggagcg ctccgccgga cttgtcatac actaagcgta gcgtataagc
      721 caaatcacct caagagcttc gtgatcatca agccggttcg cttattaggc gcgccaggta
      781 gcgggcgctg cggcgggaca ggccgtgccg gtcagtcgtg cggaagaaac tgatattcgg
      841 attgctgctc agcatatcct tcagctgcgc cttcggcacg atgacgtcca gccggccgtg
      901 cacgatatcg gtctgcacgg cgatatcctt cagctcctcg aaactttcct gggcgatgat
      961 ggcattccgc aggctgcgct cgatgggcag ccacgtctga tcgctcgcca gaaaggccgt
     1021 ccacttcttg gaaatacggg ccaccagccg tgtcatggtc agccggccgg ccggattggc
     1081 ggcgatgcgt gcgtacagat ccaggtagaa tttgcgccgc cgggcgtggc ttttgaactc
     1141 cggcagatcc gcgaacagcg gcggctcgta tagcagcagc cgctctaccc tggccggcgc
     1201 catagccgtc aaccggctgg ctaccaggca gcccatggag tgtccgacga ccaggcagtg
     1261 gcggaccttt aagcgcttca gcaacgccag cacgctgacc gcctgatcgg cggttgagta
     1321 ggtcaggtac ggcggccggg gagacgcgcc atggcccagt aaatcgggta cgatgagtgt
     1381 aaaacgacta tggtcgagct cgtcgataag cggctgccag aacgaaccgc tgctggcgat
     1441 gccatgaagc aacaggaccg taaccggccc gttcccatac acttcgtaat gcagtttgaa
     1501 cggacgcttc agccggtcat gccagaattg agcccacatc gcggcctcac gtctgcctgg
     1561 cggctttgag gagccgggca aaccacaaca gctcgtcgaa cagcttctgg acgttgtgct
     1621 cgtaacgggt gatgtactcc ggtttcatct gccctgcctc atcgaatata tcctgcaccc
     1681 gtgggaagta gacgtcccag ctcagggcta ccaagccggt ttcgcgtacc gccgtcagca
     1741 ggctctccac cgcccgtacg ccgccccagt taccattgct ggcgcccgcg aaagccaccg
     1801 gcttgtgatt gtacagttcc agctcgctgt cgagcatgcg cttgagcgag cccgggaagc
     1861 tgtgattata ctccggcgtc accacaaaga atgcgtcggc cttggcgacg atgtcggtat
     1921 agcgcgggtc cttgccctcg gggtcgttgc cgtcgcccgg gaagttgaat tctttcgggt
     1981 caacgaaaat gacgtcgaca cccggctggc gggcggcgaa gtcggcgatg tagtgcgcgg
     2041 ctttgataga ttcacgtttt tcgcgggtag tgccggccag tacggccacg gtcagtcggt
     2101 catccatagc atccatgata taggcatgtg acctgggtcg caaacggcct taatgccaat
     2161 gcgcctcgat ctcgtggatg cggtcgtggt ccaaaccgta gtagtggccg atttcgtgcc
     2221 acaaggtatg cttgatctgc gccttcagcg ccggtacatc cggtgagctc atgagcatgg
     2281 gaattttgaa gatggttata cggtccggca acagcggctg attcccttgc cgaaaggcca
     2341 acgggatgcc ctcgtacaat ccgtacagtg actcacccat gtgcaggttg aggcggaccc
     2401 gttgctcggg cgtcggttcg tcctcgaaca atatggctac gttcttcagg ccatcggtct
     2461 gctcgcgcgg aagctcaccc agcgcttcgt caatcaactc ttgaaaccgg tcatcagaaa
     2521 tgtcgagcat gctcattact atactctata tagtaatgga tgcaaccgct tgcgctagga
     2581 acccgggccc gtcatgctca tgaggtccat atggaaccaa tcgttggccg gcatattcgg
     2641 gttgtagacc acgaaagcga ggctgtcacc gctggtctga gcggtgtaac tgacgctgag
     2701 ttggtgccaa ttggtgtcgc tcagagtctg agcggtggag ccgctgctga cgacattccc
     2761 gttcgcgtcc ttctcctgga tcatgatgat gagcttctgg ttggctactt cgctcctgag
     2821 ccaggcactg gcggtgtagg tatcgcccgc gaccgccgaa gccacggggt ccggtttggc
     2881 gcggatgccg gccgctccgg ccgtggtggt attccgcagc aattggatgc tcgccgagcc
     2941 atcgtaggca tcggtcgtga tgcggttagc cttggacagc gagttataca ggcccgtcca
     3001 accggtcagg ctcgattcga cgctcggatt ggtgatgtac tcgaccgttg tcggtgtagt
     3061 ggttacggtg tcggtgtcgc ttacctgaca gacgctgccg ctgttctgac aatcggcggc
     3121 ctgaacggtg gcgttcgcgc taaagctact gccccctgcc ccgctggcca gttgcgccgt
     3181 gacggtctgg gtccatggct gtccgctgac tatgtcggca ctcggccagg tgacggtctg
     3241 gccgttcacg gttccgccgg cggcatcggt tatggtgaca ttgctgggca atgtcaggct
     3301 cggcgtcacg cccgcagcgg tggtgccgct ttgggtgtca ctgaccgtca gttggtagtg
     3361 cagcgcgtcg ccgacgctgg ccgaactgac gccgtcgtcg agagcggtcg caagcaccgg
     3421 tgtcgccggc gtctgatccg cctggtagcc atgcagagtc aggctgtaca acacgctgtc
     3481 tttggtgcac ggttctttgt tcgggttcag cgtgaagacc ggcccgcaga tgaagttaag
     3541 cgtcatgcta ttgccggtga ccgtggtgcg catgtgctcc gggtggccgg tgaaccactg
     3601 ggtgccggca atgctgctca gtgacatttc ttcgctacca ccacccccgt tcgtcacatt
     3661 gacgacgcca tgctgcggcg agaagacttc cccgccgtgt acatggtggg cgacattcag
     3721 cacgtatttg ccgtccgggc gggcgcttgg cgagtacttg tcgcccaact cgttcactac
     3781 cgtggtaacg gccgccgaga cctggttcgt gctgctgctg tatgccggcc ggtggcccat
     3841 cgtcacgatg aagtagatgt tcggatcggc ctcagcttgg gccatcagct gatcggcggc
     3901 cggctcccaa tcggtgtagg cgttgtacca ggcctcgggc atctgaatga atagcacatg
     3961 accagccgtg aagtaccccc agtcatcgcc tcggcagccg ttgacggtcg gatcggtcgc
     4021 cgacggacag cccggcgcct tcacgttcgt cgcggtgtcg ttgctgatag tctgggcgtt
     4081 cggaatattg aaccggcctt tgtagttcgc catcgagtcc ttgggtgttc cgggaggggc
     4141 gtcggccgac gggctgccgt actcgtggtt accccatacg aactgcatcg atgtctgctc
     4201 gctgaccgtt tcaatgtcat tccacagccg gtgtaccgat ggctggccgc actcgttctc
     4261 gtagtcgatg tcgccgccgt gggtcacgaa gttcggctgg tcattcgcca actgctgcca
     4321 gacttccggc atccacgtct tgttgcacga agccggcgca tcgctctcgt accaggtact
     4381 gccggtgtcg cccacgtcgt cccaggtgaa atcaccggtc ggttcggtct ggaaggtgtg
     4441 gtcgaagccg ctcgtgccga tctggtagtg gtacgtggtg ccgggtgtca ggccggtcag
     4501 tttgacctgc cagaacggac cggtcgtgcc gtcgaccggc tggaccgagg ggttcaccgc
     4561 cgtcgcggat tgggtgtaga tgttggcggt cagtccgtag ttcagcgtat tgtccggccc
     4621 gtgccagtac acccactctt cggtggcgtt atcaccgaag ctgctgtgga tttcatcgtt
     4681 cggctcactc agggccgcgg cgcccggcgc agcctgcagc gccgctccga atacgaacag
     4741 taagctgata agcacgaaca ggccccgctg tatctgctcc gttcccaccc ggagtaacct
     4801 ttgccgtgca accatattta ttctcccaaa ccgctaatag tattagcagt gtacgggttc
     4861 tgttcgttct tgcaaagata taaaaaatac agcaggtcca tagctgagaa cgatgcatgc
     4921 tgcccctgtt aggaatatat ctgtaccacg cgtgtcagca aaagcgtgaa cacgacgctc
     4981 gctaccacga acaggatggc gtagtgctta cggacgaacc ggtgcagcag cggatagatg
     5041 gcaaccgtgg caccataggc caccaaatac cgggccatcc gggccacgat agcgatacct
     5101 atgaacagcg gaatataaaa atgccacccg gcggcttcgt gggtgaagac cttgtagggc
     5161 acgccggaaa acggctggtg ggccaatccc caaacaccgg ccgaggcgta ccagtcatgc
     5221 acctgggtca tcatggccgg ccggatgaac ggcagccgca gaaatgcctg cgccggcaga
     5281 tgccacacca ggccgataag cgtgccggca atggtcccga acaagtcgta cttcaccagg
     5341 tcgaacttac ggtgcacctt catgaaaacc accaaaaaca gcagaaactc gggaatgata
     5401 taccagacca gcgcttcccc ggcggcccac acgaagacca gtgcgttgaa ttccggacta
     5461 ccgaaaaatc gctccatgaa gtagttgaaa tgcggccgta ccgccaggat ctggcggcgg
     5521 tgccggtacg cgaacaggcc gcagaacggc aaaaagaaca gcagcatacc gccgaatacg
     5581 gccaagaagt tcagcccggc gcttaagaaa tacgcagcca taagatcacc agtgcgtcga
     5641 ataccagctg gaactgtaca gcctcatgaa agatacgatt cagcgaagcg tagtcgtggg
     5701 ctgcgaacag ccgcgcgcgc aacaccataa gccctaccac tacgatgcag agactgcctg
     5761 caacgctcaa ttggcgcgta tcggttacgt aaaccgtcag gatcgtcaac gacagcagac
     5821 cggccgcggc cagcccgaag ctgagacgag cggtcagcgt gcccccgtag cgcagcagga
     5881 acgtacgctt gccggcctgg gcgtcacccg gcgcatcttt gtagtctttg agtagcgata
     5941 acgatgcccg gctcagcatc cacgccaaca gcatcagcca caccaggcgg gtcggctgcc
     6001 ccagcccggc gcccatcagg aacggcagcg ccccgtagga cagcgccagt atcaccatgg
     6061 acaataccgg ccgccgtgaa gcccggaccg gttcccggtt atacagccag cccagcccga
     6121 acagcaccag ggtcagcagt acttcggtga gtggcaggaa acgcagcaga acgacaaccg
     6181 ctgccaccag gatgtacagc cctgcccgaa ccatcgtcag cagcccctgt ttgcggagcg
     6241 ccgcgacccg gtggatatcg ttatagtggt cccctgcttc gtccaccagg tcgttccata
     6301 ccgtgacgat cgagtgcact agaaccacga cggccgcaac cataaacacc cgccccgagt
     6361 cattcgtacc ggcagcgaac gcaccggc
//
LOCUS       DASSFD010000188         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1607942, whole genome shotgun sequence.
ACCESSION   DASSFD010000188 DASSFD010000000
VERSION     DASSFD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607942"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFH39_04605"
     CDS             complement(<1..728)
                     /locus_tag="VFH39_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6925085.1"
                     /translation="MTIFLFILGIVLFIGLVVAHEWGHFIMARRGGVEVEEFGIFFPP
                     RLFKKRTKGGWDFTINLLPLGGFVKLKGEHDSDTGKGTFGAASLKTKSKITAAGVVVN
                     LAIAYLLFLLLAFIGLPKLVPDQFTVASDTKYVHQASYSVKADTIEKGSPAAQAGIKP
                     DDTVLALGPAGHLTKVLQPQQLTDLTDKYAGQTVELKYEHNGKTVEKTVALRSDAAVA
                     ALTKQSKPAGHLGLSVYPTQAGLTV"
     gene            complement(795..1490)
                     /gene="uppS"
                     /locus_tag="VFH39_04610"
     CDS             complement(795..1490)
                     /gene="uppS"
                     /locus_tag="VFH39_04610"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET6925086.1"
                     /translation="MNTAADVPNHLGFILDGNRRWARAHGLPTLEGHRKGYDNLWPLL
                     DAALAKGITYLSAYAFSTENWKRSQEEVRYLMNLILLIVTRDLKKLQERNMKLLWIGS
                     KQGMGKKVQAALEKAEQATAGNTRGLLGLCINHGGRREIAEAAQRLVRDGLRPEEITE
                     ATFAEYIDHPELPPLDLVIRTSGEYRLSNFMLWRAAYAELIFVDKCWPDYTPADLDEA
                     LEEYARRHRRHGS"
     gene            complement(1507..>2046)
                     /gene="frr"
                     /locus_tag="VFH39_04615"
     CDS             complement(1507..>2046)
                     /gene="frr"
                     /locus_tag="VFH39_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET6925087.1"
                     /translation="DANTKFQAASEHFKSELTRLRTGRASAGMLDGVMVEAYGTAMPL
                     NQVATVTAPEAQLIQITPFDPNNLQAIAAAIRNNQSLGLNPTDDGRVVRVPIPPLTEE
                     RRREISKQVGSRQEECMVTLRNIRHDAMDAIDKAKKDKEIGEDDAKRLQKQVDDAMNS
                     TRDAVTAAAKAKEQEIMSL"
BASE COUNT          352 a          647 c          586 g          461 t
ORIGIN      
        1 acggtcagcc cggcctgcgt cggataaacg cttaagccaa ggtggccggc cggcttgctc
       61 tgcttagtca gcgccgcgac cgcggcgtcc gagcgcaagg ctaccgtttt ctccactgtt
      121 ttgccgttat gctcatactt cagctccacc gtctggccgg cgtatttatc ggtcagatcc
      181 gtcaactgtt gcggctgtaa tactttcgtc agatgcccag ccggtcccaa agccaatacc
      241 gtatcgtccg gcttgatacc agcctgggcg gccggagatc ctttctcgat agtatcggct
      301 ttgacgctgt aggatgcctg gtggacatac ttggtgtcgc tggctacggt gaactggtcc
      361 ggcaccagct tgggcagacc gatgaaggcg agcagcagga acagcaagta ggcgattgcc
      421 agattcacca ccacgccggc ggcagttatc ttcgacttcg tcttgagact ggccgctccg
      481 aacgtgccct tgccggtgtc gctgtcgtgc tcacccttca gctttacgaa accaccgagc
      541 ggcaacaggt ttatggtgaa gtcccaaccc cccttcgtcc gcttcttgaa caagcgcggc
      601 ggaaagaaaa tgccaaactc ttccacctcg acgccgccgc gccgggccat tatgaagtgc
      661 ccccactcgt gcgccactac caggccgatg aacaggacga tacccaagat gaacagaaag
      721 atggtcatat tctttaccat tataccggtg gcccgctgag aataaaatga gcggcctcac
      781 ttcgtaccaa cgattcagct gccgtggcgc cggtgccggc gggcgtactc ctccagcgct
      841 tcgtccaggt cggccggagt gtagtccggc caacatttgt cgacgaagat aagctcggca
      901 tacgcggccc gccacaacat gaagttactc aggcgatact ccccgctggt gcggatgacc
      961 aggtcgagcg gcggcagctc cgggtggtcg atgtattcgg cgaatgtggc ttccgtaatt
     1021 tcttcgggcc gtaaaccatc acgcaccaac cgctgcgccg cctcggcgat ctcgcgccgg
     1081 cccccatggt tgatgcataa tccgagcagt ccgcgggtat tgccggccgt cgcctgctcc
     1141 gctttctcaa gcgccgcctg gactttctta cccatcccct gtttgctgcc gatccacagc
     1201 agcttcatgt tgcgctcctg cagcttcttg aggtcacgtg tcactatgag caatatgaga
     1261 ttcataaggt atctcacttc ttcctggctg cgcttccagt tctcggtcga aaacgcgtag
     1321 gccgacaggt aggttatacc tttggccagg gcggcgtcca gcagcggcca gaggttgtca
     1381 tagcccttgc ggtgcccttc caaagtcggc aggccatgcg cccgcgccca gcgccggttc
     1441 ccgtccaaaa taaagcccag gtgattgggc acatccgctg cagtattcac gtcttccgga
     1501 tgagccttac aggctcatga tctcctgctc tttagccttg gcggcagcgg tcacagcgtc
     1561 gcgcgtgctg ttcatggcgt cgtcgacctg cttctgcagg cgcttggcgt cgtcctcgcc
     1621 gatctctttg tctttcttgg ctttgtcgat ggcgtccatg gcatcgtgac ggatgttgcg
     1681 cagcgtcacc atgcactcct cttggcgtga accgacctgc ttgctgattt cgcgccggcg
     1741 ctcttcggtg agcggcggaa tcggcacccg tacgacccgg ccgtcgtcgg tcgggttgag
     1801 ccccaggctc tggttgttgc ggatggcggc ggcgatcgcc tgcaggttgt tcgggtcgaa
     1861 cggcgttatc tgaataagct gcgcctcggg cgcggtcacg gtcgccacct ggttcagcgg
     1921 catggctgtg ccatacgcct cgaccatcac gccgtcgagc atgccggcgc tggcccggcc
     1981 ggtccgcaac cgcgtaagct cacttttgaa atgttcactg gcggcttgga acttcgtatt
     2041 ggcatc
//
LOCUS       DASSFD010000189         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_39688, whole genome shotgun sequence.
ACCESSION   DASSFD010000189 DASSFD010000000
VERSION     DASSFD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39688"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFH39_04620"
     CDS             <1..192
                     /locus_tag="VFH39_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925088.1"
                     /translation="GGLVAYIQQAIVLREDEEEMEALADAKPSKDVDRIPEAVLVENP
                     KPKTKTSSKSAKNSKRRKR"
     gene            189..662
                     /locus_tag="VFH39_04625"
     CDS             189..662
                     /locus_tag="VFH39_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET6925089.1"
                     /translation="MNESSDALEAAILYAKKFLEDLLSFFGVNMDVYATRDDEVIQLS
                     VPSTQLNGFLIGQHGETMRSLQYLVSTALKNNGHEYTRVNVDIADYKRQRADRLAHTA
                     EDWIKQVKDSGEPLELKPMNAADRRIVHKVASDNGLMTESVGEGRERHVVLKPGA"
     gene            complement(726..1826)
                     /locus_tag="VFH39_04630"
     CDS             complement(726..1826)
                     /locus_tag="VFH39_04630"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6925090.1"
                     /translation="MSTIAIDARELRQSTGRYVERLIHYLQQIDTEHDYVILLKPDDM
                     DTWQPTNPRFKKVACPHKEFTFAEQLGFKKQLNQLKPDLVHFASVQQPIWYRRPVVTT
                     MQDLTTVRFRNPTKNYIVFSLKREVYKWVNRRAARKSRLLITPTQYVKDDVVGFTRVS
                     PDKITVTHEAVDDFDEAPAPVPGFEGKRYIMYNGRPLPHKNLRRLIEAFAILAPKYPD
                     LHLMLAGKKNALQDSYVELATKLGVGDRVVLTDWITDGQLKWAMQHAVAYIWPSLSEG
                     FGLPPLEAMLNGTPVVSSNATCMPEVLGEAAYYFDPLSSEDMAAKIGEVVEDPILREE
                     LVDKGRQQIQKYSWQRMAEQTLDVYRRALGEK"
     gene            complement(1823..2614)
                     /locus_tag="VFH39_04635"
     CDS             complement(1823..2614)
                     /locus_tag="VFH39_04635"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET6925091.1"
                     /translation="MTDDQLQAVIEVVRRAGAVLMEEFGSDELGAETKPDGSPVLRAD
                     KRSSALLEAELPKVIDVPVLSEENEHRELSADAPYFIIDPLDGTKEFMRGSHDFAINV
                     AFMEGGLPTAGVVYGPARNELYYARQGQGAFRIRGSETEAIHVSDRQRELIGIMSFSH
                     ASERDTELLDKLGCTSTIRMGSVLKFCAVASGAADIYMRSGRTMIWDTAAADIVVREA
                     GGHMLYYTGDRLSADFNLSLANDGFLCSNIDINPSDVAQYFGGQA"
BASE COUNT          575 a          816 c          859 g          549 t
ORIGIN      
        1 ggcggcctgg tggcctacat acaacaggcc atcgtcttgc gcgaagacga ggaagagatg
       61 gaagccctgg ccgacgccaa gcccagcaag gatgtcgacc gcatcccgga ggcggtgctc
      121 gtagagaacc cgaagccaaa aaccaaaacc agcagcaagt ccgccaaaaa cagtaaaagg
      181 aggaagcgat gaacgaatcg agcgacgcct tagaggctgc catactgtat gccaagaagt
      241 ttttggaaga cctgttgtcg ttcttcgggg tcaacatgga cgtttatgcc acccgcgacg
      301 acgaggtcat ccaactgagc gtgccctcta cccaactgaa cggcttccta atagggcagc
      361 acggcgagac gatgcgatcg ctgcagtacc tggtgagcac ggcgctcaag aacaacggtc
      421 acgagtacac ccgcgtgaac gtcgacatcg ccgactacaa gcgccagcgg gccgaccggc
      481 tggcccacac cgccgaagac tggataaaac aagtaaaaga cagcggcgag ccgctggaac
      541 tgaagccgat gaacgccgcc gaccgccgga tagtgcacaa ggtggccagc gacaatggcc
      601 ttatgaccga atcggttggc gaaggccgcg agcggcacgt cgtcctgaaa ccgggcgcct
      661 agcgcccctc ccctagctac cataaacact taacttgcta agtatttaga acgctgccaa
      721 ctggcttatt tttcgccaag ggcccggcgg tagacgtcca gcgtctgttc ggccatgcgt
      781 tgccaggaat atttctgaat ctgttggcgg cctttgtcga ccagctcttc acgcaggatg
      841 ggatcctcca cgacctcgcc gattttggcg gccatgtcct cgctactgag cgggtcgaag
      901 tagtaggcgg cctcgcccag cacttccggc atgcaggtag cgttgctgct gaccaccggg
      961 gtaccgttga gcatcgcctc gagcggcggc agaccgaagc cttcgctcaa gctgggccag
     1021 atgtaggcga cggcgtgctg catagcccac ttcagctgac cgtcggttat ccaatcggtc
     1081 agaacaaccc ggtcgccgac gccaagcttg gtcgccaact cgacatagga gtcctgcagc
     1141 gcgttcttct tgccggccag catgaggtgc aggtcgggat atttcggtgc caggatagcg
     1201 aaggcttcaa tcagccggcg caggttcttg tgcggcaggg gccggccatt gtacatgatg
     1261 tagcgcttgc cctcgaagcc aggcacgggt gccggagctt catcgaagtc gtcgaccgcc
     1321 tcgtgagtaa cggtgatctt atccgggctg acgcgtgtga accctaccac gtcgtctttc
     1381 acgtactggg taggagttat aaggagtctc gacttccggg ccgctcgccg gttaacccat
     1441 ttgtagacct cgcgcttcaa gctaaagacg atatagttct tggtcgggtt acggaagcgc
     1501 acggtcgtca ggtcctgcat ggtggtgact accgggcggc gataccagat tggctgttgc
     1561 acgctcgcaa agtgtacgag gtccggttta agctgattga gttgcttctt gaagccaagt
     1621 tgctcggcaa aggtgaactc tttgtgcggg cacgcgactt ttttgaagcg cgggttggtc
     1681 ggctgccagg tgtccatgtc gtccggcttg agtagaatga cgtaatcgtg ctcagtatcg
     1741 atttgctgca gataatggat gagccgctcg acgtaccggc cggtactctg gcgcagttcg
     1801 cgggcgtcga tagcgatggt gctcatgcct ggccgccgaa atattgcgct acatcggacg
     1861 ggtttatatc gatgttgctg cacaagaagc cgtcgttggc caggctcagg ttgaagtcgg
     1921 ccgacagccg gtcgccggtg tagtacagca tgtggccacc ggcttcgcgc accacgatgt
     1981 cggcggcggc ggtgtcccag atcatcgtcc ggccgctgcg catgtagatg tccgccgcac
     2041 cgctggcgac ggcgcagaac ttcagcaccg accccatgcg gatggtgctg gtgcagccga
     2101 gtttatcgag cagttccgta tcgcgctccg aggcatggct gaagctcatg atgccgatca
     2161 gctcacgctg acggtccgag acgtggatcg cttcggtttc actgccacgt atgcggaagg
     2221 caccctggcc ctgccgggca tagtacagct cgttccgtgc cggcccgtac acgacgccgg
     2281 ctgtcggtag gccgccttcc atgaaggcga cgttgatggc gaagtcgtgc gagccgcgca
     2341 tgaactcctt ggtgccgtca agcgggtcga tgatgaagta cggcgcatcg gccgacagct
     2401 cgcggtgctc gttctcctcg gacagtaccg ggacgtcgat gaccttcggt agttcggctt
     2461 ccagcagggc gctggaccgc ttgtcggccc gcagcaccgg cgagccgtcc ggcttggtct
     2521 cggctccgag ctcgtccgag ccgaactcct ccatcagcac cgccccggcg cgccggacaa
     2581 cttctatgac tgcttgcagt tggtcatcgg tcattaccgt tacatggtaa tatatgaggg
     2641 ctgataatgt aatggtggcc ggcgtaaaaa cgtcagccga ttctcatggg gacgcgtcac
     2701 acttggcacg gcactacgca ggaaacaagg aggaaacaca agagcatgaa cgaaccgcac
     2761 ggtggcaaac tgatcgacct gatggtctcg cccgaaaag
//
LOCUS       DASSFD010000190         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_717851, whole genome shotgun sequence.
ACCESSION   DASSFD010000190 DASSFD010000000
VERSION     DASSFD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717851"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFH39_04640"
     CDS             <1..1146
                     /locus_tag="VFH39_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6925092.1"
                     /translation="KKPKKTVKPLHLNPWQKKFFTVLVFTKLNTRRFFRDRLALFFGI
                     LFPLIFLAVFGSLTRGNGNVTFHVALINQSQQPFAMQYAKSLQGNATFKVDTAVTNFD
                     DAKEKMTRNQLDGTVILPPSFGEVKNGVPTGQAKVYYTVSNEQSGQTLATVLQSTLQP
                     INAKYVSIQLPLTVGKNELRNKSLKPFDYTFAGLLGFSIIGIGIFGPINVFPELKKQG
                     ILRRLHTTPIRVWQYFMSTMFSQAITGVVSIAIQFVVAISVFHLNVVGNYFEIILFTI
                     LSIFMILGIGLAIGGWAKNERQAAPLSNIIVFPMLFLSGTFFPRYAMPEWLQHITNYL
                     PLTPVIDGLRLLITEGKHLTDIGPQLGLIGAWMIIIYIIAFRVFRWE"
     gene            1192..1926
                     /locus_tag="VFH39_04645"
     CDS             1192..1926
                     /locus_tag="VFH39_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925093.1"
                     /translation="MASENQTGAPSPELLPPKVDLLDRMYTLYATAGFYPTTRTEINE
                     AFGLLDEGRPKVATVRHLDDVYRHQLKADTNNPEAAVRNITRLYVNYAQKARADVGAL
                     VFLEREIHDPEVVNYRQSLREADIVPGRAQLARFMDLRVLAQQQSDRNLLFSPLETGR
                     KGRQQNAFDRYTAVQPDPEIQARIELVADMSIKNVMDILPAAINEQRARREFWIERLE
                     GASLYERSAPIAREALQRLGIRPVDK"
     gene            1954..2940
                     /locus_tag="VFH39_04650"
     CDS             1954..2940
                     /locus_tag="VFH39_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HET6925094.1"
                     /translation="MPSSVRDLPKPFFVLAPMDDVTDTVFRQIVQQTAPPDLFFTEFA
                     NVDGLMSPGRPKLLKKLRFAPGETRLVAQLWGLKPENFKAVAHQIADGTLAREMGLPD
                     GCNFVGIDLNMGCPAKSEVQNGACSALINNRELAGEIIRATQEGAGELPVSVKTRLGF
                     NAIDLTWHEFLLGFKLSMLTIHGRTRKEMSKVPAHWDEIGQIRELRDRLAPETLIVGN
                     GDVMNRKHGVELAERYGLDGIMIGRGIFHDPYAFAHDSPWETIAKPERVELYTKHVKL
                     FRNTWNDGERPVYTLNKFCKIYINGFDGAKELREQLMGAGDAAELQTMLAVV"
     gene            2988..>3244
                     /locus_tag="VFH39_04655"
     CDS             2988..>3244
                     /locus_tag="VFH39_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925095.1"
                     /translation="MLLSLLSKFLASLLVAAITLATLVVLFSLTLLNSHYIEGQLSAT
                     HTYSRLSVALSDELVKQASDQGDQVDPALKQELQGALSPVV"
BASE COUNT          723 a          966 c          886 g          669 t
ORIGIN      
        1 aagaaaccca agaagaccgt caagccgctg cacctcaacc cctggcagaa gaagttcttc
       61 accgtgctcg tctttaccaa gctcaacacc cgccgcttct tccgcgaccg gctggcgctg
      121 ttcttcggca tcctgttccc gctcattttc ctggccgtgt tcggatcgct gacccggggc
      181 aacggcaatg ttacgttcca cgtggcgctc atcaaccaat cgcagcagcc gttcgcgatg
      241 cagtacgcca agtccctgca aggcaatgca accttcaagg tcgatacagc ggtgaccaat
      301 ttcgacgacg ccaaggagaa gatgacccgc aaccaactgg acggcacggt cattttgccg
      361 cccagcttcg gagaggtcaa aaacggcgtc ccgaccgggc aggccaaggt gtactacacc
      421 gtcagcaacg agcaatccgg ccagacgctg gccaccgtcc tccaatcgac cctccagcct
      481 atcaatgcca agtatgtgag catccagctg ccgctcacgg tgggcaagaa cgagctgcgc
      541 aacaagagcc tcaaaccgtt cgattacact tttgcgggcc tgctcggttt ctcgatcatc
      601 ggtatcggca tcttcgggcc catcaacgtc ttcccggagc tgaagaagca gggtattctg
      661 cgccggctgc acaccacgcc tatccgcgtc tggcagtact tcatgagcac catgttcagc
      721 caggccatca ccggcgtcgt gtccatcgcc atccagttcg tggtcgctat cagcgtcttc
      781 cacctcaacg tggtcggtaa ttacttcgag atcatcctgt tcaccatcct gagcatcttc
      841 atgatcctgg gcatcggcct ggccatcggc ggttgggcca agaacgaacg ccaagccgcg
      901 ccgctcagca acatcatcgt ctttcccatg ctgttcctgt ccggtacgtt cttccctcgc
      961 tacgccatgc cggaatggtt gcagcacatc accaactacc tgccgcttac cccggtcatc
     1021 gacgggctgc gtctgctcat taccgaaggc aagcacctga ccgacatcgg cccgcagctt
     1081 ggtctcatag gcgcttggat gatcatcatc tacatcatcg ccttccgcgt cttccgctgg
     1141 gaatagcgtc gaaatattgc aatagataag caaaagtgtt ataatacttg catggcttcc
     1201 gaaaatcaaa ccggtgcacc gtctcccgaa cttttgccac ctaaggtaga tttgttggac
     1261 cggatgtaca ccctgtacgc caccgccggt ttttatccca ctactcggac ggaaattaac
     1321 gaggctttcg gcctccttga cgaaggcagg cccaaggttg ctaccgttcg ccacctggac
     1381 gacgtctacc gccatcagct caaggcggac acgaacaacc cggaagcggc agttcgtaac
     1441 attacgcgct tatacgtcaa ttatgctcaa aaagcccggg cagacgtagg tgcgctagtt
     1501 ttcctagaga gggaaatcca cgacccggag gtggtgaact accgacagtc actacgtgag
     1561 gccgatatcg tccctggccg agcgcagttg gcacgcttta tggacttacg ggtgctggca
     1621 caacaacaat cagaccggaa cttactgttc agcccgctcg agacggggcg taaaggccgc
     1681 caacagaacg ccttcgaccg gtacacggcg gtccagccgg acccggagat tcaggcacgc
     1741 atagaacttg ttgccgacat gtccatcaag aatgtcatgg atatcttgcc ggccgccatc
     1801 aacgaacagc gagcgcgtcg tgaattttgg attgagcgtc tcgagggagc ctcattgtat
     1861 gaacgctcgg ctccgattgc ccgcgaagct ttgcagcgtt taggtatacg ccccgtcgac
     1921 aaatagctgc acgttatata ctagatagta attatgccca gtagtgtaag agacttaccg
     1981 aagccattct tcgtgttggc gccgatggac gatgtgacgg acacggtgtt ccggcagatc
     2041 gtgcagcaga cggcaccgcc ggatctgttc tttacggagt tcgccaacgt tgatggcctg
     2101 atgagtccgg gccgcccgaa gttgctcaaa aagctacgct tcgctccggg cgaaacccgg
     2161 ctggtggccc agctgtgggg gctgaagccg gaaaacttca aagcggtcgc gcatcagatt
     2221 gccgacggaa ctctggcgcg cgagatggga ctgccggacg gctgcaactt cgtgggtatc
     2281 gacctgaaca tgggttgccc ggccaagagc gaagtgcaga acggagcgtg cagcgcgctt
     2341 ataaacaaca gggaactggc aggggagata atccgtgcca cgcaggaagg ggcaggggag
     2401 ctgccggtga gcgtcaagac gcggctgggc ttcaacgcca tcgacctcac ctggcatgag
     2461 ttcctgctcg gctttaaact tagcatgtta actattcatg gccgcacccg gaaggaaatg
     2521 agcaaagtgc cggcgcattg ggacgaaatc ggtcagatcc gcgagctgcg tgaccggctg
     2581 gcccccgaga cgctcatcgt cggtaacggc gacgtcatga accgcaagca tggcgtggag
     2641 ctggctgagc ggtacgggct ggatgggatt atgatcggcc gcggcatctt ccatgacccg
     2701 tacgcgttcg cccatgattc gccgtgggaa acgattgcaa agcccgaacg ggtcgaactt
     2761 tatactaaac atgttaagct ttttcggaat acctggaatg acggcgaacg cccggtgtat
     2821 acacttaata agttctgcaa gatatacata aatggcttcg atggagccaa agaactgcgc
     2881 gagcaactta tgggcgccgg tgatgcagcc gagctgcaga ccatgctcgc tgtagtgtga
     2941 cccacgctgg cgcattgcca ccctgcccgg tacaatgaat gctgctcatg cttttgtcgt
     3001 tgctctctaa attcctggct tcgctgctgg tagccgccat aacattggcg acgctggtag
     3061 tgctcttcag cctgacgctg ctcaacagcc attacataga gggccagctg tcggcaacgc
     3121 atacctatag ccgcctgtct gtcgcactca gcgacgagct ggtcaagcag gcatccgatc
     3181 aaggcgatca ggtcgatcct gcgctgaaac aggaactgca aggagcgctc tcaccggtgg
     3241 tcat
//
LOCUS       DASSFD010000191         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1735955, whole genome shotgun sequence.
ACCESSION   DASSFD010000191 DASSFD010000000
VERSION     DASSFD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735955"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFH39_04660"
     CDS             complement(<1..954)
                     /locus_tag="VFH39_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925096.1"
                     /translation="MAVLFASCLFPAGCSSNTFDALSAINDTIVKIHQDPEAVTKFLS
                     DLEHQLLANASNSIKDLVTAVSDLATRSAVTAAAGGMCSVQYVEENSVVALEEIKHDF
                     FHELAPTQVTSVCLGQPNPVDEQSVLKGDTKSVLFYGYALDVPKDLSADIKESDGTRK
                     KVPAELVSRTSPYFATVILDTTSGRPLVDSQSVGAILVADGKDISTVGITPPVRDELI
                     HLTITFTSSSYSLLPVIGNFAITTVSILGLQPITITGVPVTENLPNLLAAQASASDIS
                     GRALQICWMPVDASLAFTQIRWDFSYNLTAERPSGKQYIVTG"
     gene            complement(1579..1779)
                     /locus_tag="VFH39_04665"
     CDS             complement(1579..1779)
                     /locus_tag="VFH39_04665"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6925097.1"
                     /translation="MTVHPLGGAPMADNPAVGVVDDTGKVYGYDGLYILDGSIVPTAI
                     GVNPSKTIAALAERGVERLIQE"
     gene            1978..>2294
                     /locus_tag="VFH39_04670"
     CDS             1978..>2294
                     /locus_tag="VFH39_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925098.1"
                     /translation="MLRHEVMVLRRQVAHPTPDWADRAVLAALARLLPGALRGSCLVT
                     PGTLLAWHRRLVTRKWTYPEPVGEVHDQVARLLGRPWSVRVRGSTRRAPGGIHTRDCP
                     PPAA"
BASE COUNT          466 a          644 c          670 g          514 t
ORIGIN      
        1 gccggtcacg atatattgtt taccgcttgg cctttcggct gtaaggttat agctgaaatc
       61 ccatctaatt tgcgtgaagg caaggctggc atcaactggc atccagcaaa tctgcaaagc
      121 gcgcccagat atgtctgatg cactagcttg cgcggccagg agattcggca gattttctgt
      181 aaccggcaca ccggtgatag ttataggttg gaggccaaga atggaaacag tggttatagc
      241 aaaattacct atgacaggca acaacgaata acttgacgag gtaaatgtga tcgtcaggtg
      301 aatgagttca tcgcggactg ggggcgtgat accgacagtg gatatatcct tcccgtcggc
      361 tactagtatg gcccccacgc tctgagagtc tacgagtggt cgtcccgagg tggtgtccaa
      421 gatgacagtt gcaaagtacg gtgacgttct agataccagc tccgcgggga cttttttcct
      481 cgttccatca gactctttga tgtcggccga taggtccttc ggcacatcca gggcataacc
      541 gtagaacagc acagacttcg tatccccctt cagcacgctt tgctcatcga cagggttcgg
      601 ctgcccgagg cagactgacg ttacctgcgt aggcgccagc tcatggaaaa aatcatgttt
      661 aatctcctcc agggcgacaa cggagttttc ctcgacatat tgaactgaac acattcctcc
      721 tgctgccgcg gtcacggcag acctggtggc caggtcggag accgcagtaa cgagatcttt
      781 gatcgagtta ctggcgttgg ccagaagctg atgttccagg tcgctcagga actttgttac
      841 cgcttcaggg tcttggtgaa tctttacgat agtatcatta attgcactaa gagcatcgaa
      901 agtattagaa gagcagccag ccgggaacaa gcacgacgcg aacaggacgg ccacaagcac
      961 ccatcgggaa cggctgcctt gcttccacgc ccaagttcta ggaataggcg ttgccggacg
     1021 gcatattatc cggcaagcaa gaagcgccgt acgggctgag ctgaaccaaa gcatccggac
     1081 cccaggtatg taatgagtgc attgatttct aagacaggga atgccgctct ccaagcagga
     1141 gaccagctcc ttggcgtcca cgacctcaca gtccgtacat aaccccagga caatctggaa
     1201 gtcaagtgtc ttgctggccc tgactgtgca cgagccgccc gctcatgact aggtgccgga
     1261 tacgacggga tcggcgtcgg cttctgttcc ggtcaggcag gccgtcgacg gtcgaggatt
     1321 tcgatacgga cactcgcatc cgcaacacgc tgttttgctc ctgcggcttg gcgaccgcgg
     1381 attcgctctg ttcaccggtc gctagcgtac tgtgcggcct atggctgcca gtccgatcaa
     1441 aattcgcaac gtcatctggc ccgcgctcat cttaatcaat tttcgagcat gcctacatca
     1501 attgaatcgc tgagatcatt ttcagtgatg ctctttagaa gtcttttctc gtgttagaaa
     1561 ttgacccgcg gcggcccgct actcttgtat taggcgttcg acgccgcgct ctgcaagagc
     1621 ggcgatcgtt ttcgatgggt tgacgccgat ggctgtgggt acaattgagc cgtccagtat
     1681 gtatagaccg tcatagccgt ataccttgcc ggtatcgtca actacgccga ctgcggggtt
     1741 gtccgccatt ggtgcccctc caagggggtg aacggtcatg cgtgtgctaa gtggccctgc
     1801 cctgtactgc ttctaaactc ggcacaggga cgctgagctg cggtttggcc tggccagcag
     1861 gttgaggcaa gatcgcggtt cgtgcctgtc cgtctgctgt atctaatcat ggtccgggtg
     1921 ttcggctggc tggtcctgct gagccggagc caggcgtcga aggacgcgga gatcctggtg
     1981 ctgcgccatg aggtgatggt gctgcgccgc caggtagccc atccgacgcc ggactgggcc
     2041 gatcgggcgg tcctggcggc gctggcccgg ctgctgcccg gcgcgctccg cggcagctgc
     2101 ctggtcacgc cgggaacgct gctggcctgg cacaggcgac tggttacccg taaatggacg
     2161 tatcccgaac cggtcggcga ggtccatgac caggttgcgc gcctgctggg ccgtccatgg
     2221 tccgtccggg tgcgcgggtc aactcgccgt gcacccggcg gtatacacac ccgggattgt
     2281 ccgccgccag ccgc
//
LOCUS       DASSFD010000192         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1292421, whole genome shotgun sequence.
ACCESSION   DASSFD010000192 DASSFD010000000
VERSION     DASSFD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292421"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFH39_04675"
     CDS             <1..350
                     /locus_tag="VFH39_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET6925099.1"
                     /translation="AEAKNVNAQLIKLSQMVEANKLSSTAAKEVLAEVLKTGADPEQV
                     AGDKNLLQVSDEGAIAEIVAKVLAENEKAATDVKSGEMKAIGFLVGQVMKQSQGKANP
                     AMAQELIKKQLGL"
     gene            391..1356
                     /locus_tag="VFH39_04680"
     CDS             391..1356
                     /locus_tag="VFH39_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET6925100.1"
                     /translation="MYERPEYVQSVHLYPIKSCQQATVDGELPSCLRVAETGFTAYDV
                     RDREFVIAEAEPLRDHSLLFVSQRGWNETGRAEHRGDNVLASVETDITATALTVRSHI
                     GELALDSRYHDDGKEYDVRIHKYVLPNAIDQGDEAADYFSGLLERPVRLLRANRAVMR
                     RISPERRRPDASNSVAAADAYPFLLTNQATLNALHRAAGMELGTIPMNRFRPNIVIEG
                     SGVRAMGEDLMREVVIGGLYAHVVKPCDRCPIPNIDQQTGSRDVPAGLKLLKYHRGRL
                     PGDEKARLFFGQNLNPVFRSGVTVHRGDKVRVIRQEKTPNFAPVR"
     gene            1381..1935
                     /locus_tag="VFH39_04685"
     CDS             1381..1935
                     /locus_tag="VFH39_04685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6925101.1"
                     /translation="MKPFRRVEPTVVQKVGHRSVVTKHFVMPDGSTYDFQTFNAEGTE
                     FAGVVALTPDRQVITAYQYRPGPEAFMYEIPGGGTDDGDDGDMAVTATRELREETGYK
                     PGTLTYLGKVYKDAYNNATWHYFLAEDCVLDGEQELDATEAVEVRLVPIDRFLDYARN
                     GQMTDTEAVLLAYDRLMALGSVRV"
     gene            1972..2529
                     /locus_tag="VFH39_04690"
     CDS             1972..2529
                     /locus_tag="VFH39_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925102.1"
                     /translation="MPRGAQNAREHNVDEFRHCIDAVIGQVPPLPEAEQVGIAPMRRQ
                     EDATYISADRREGTPVVINRLESAHKWALLTRTSFTMYTGSLAIVSYLERPRDLDRIW
                     LIDDYRGAARIRTIKRLKTEPELQMAASLALSPVGFKRLWTELGALTQSESDFVYDKF
                     RQPGDETAALQEFSALEPELGDNGW"
     gene            2519..3448
                     /locus_tag="VFH39_04695"
     CDS             2519..3448
                     /locus_tag="VFH39_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET6925103.1"
                     /translation="MVGKPKHQGETMPQVTKAVIAAAGFGTRFLPQTKAMPKEMLPLI
                     DKPIIQYIVEDLVAAGIQDIIIVGSSNKRAIEDHFDVPNEDLLANLRAGGPKKQPFID
                     AIEAVANLANFIYVRQKGPYGTATPLMNAAHLIGNEPFIYAFADDLTVAEPNAFQQLI
                     ALHEELGGGILPCIRATEDVDYERYGIIGGEVVRDGVMRMASIIEKPGKAGAPSDFAA
                     VGGYLFTPDIFDYLEQGLRDLPAGKEFYVTDSIIEPMLRDGKPYFGAVIQNGQRYDTG
                     DKLEYLKTVIDFGLAHPELGAPLRDFIDQRLQD"
     gene            complement(3423..>3651)
                     /locus_tag="VFH39_04700"
     CDS             complement(3423..>3651)
                     /locus_tag="VFH39_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925104.1"
                     /translation="TSSGCPVRHRLPLFRSDPSTSLEPLAELFNASPERLAAPREQSG
                     IDIGLNALARLLDRALDIQELGRPSLADAGR"
BASE COUNT          812 a         1081 c         1108 g          650 t
ORIGIN      
        1 ccgcagaggc gaaaaatgtt aatgcccagc ttatcaagct cagccagatg gtcgaggcga
       61 acaaactgag ctccactgct gccaaagaag tgctggccga agtactgaag accggcgccg
      121 acccggagca ggtggccggt gacaagaacc tgctgcaggt cagcgacgaa ggcgccatcg
      181 ccgaaatcgt cgccaaagtc ttagccgaga acgaaaaggc ggccaccgac gtgaagagcg
      241 gcgagatgaa agccatcggc ttcttggttg gccaggtaat gaaacagtcg cagggcaaag
      301 ccaaccccgc tatggcccaa gagctcatca agaagcagct cggtctctaa aatattgatt
      361 acgcaacaaa acagtatata atacaagtat atgtatgagc gtcccgagta cgtccaaagc
      421 gtccaccttt accctataaa gtcctgccag caggccaccg tcgacggcga gttgccgtca
      481 tgccttcggg tcgccgagac cggctttacg gcctacgacg tacgcgaccg cgagttcgtc
      541 atcgccgagg cggagccgct gcgtgaccac agcctgttgt tcgtctccca gcgtggctgg
      601 aacgaaaccg gacgtgctga gcaccgcggt gataatgtac tggcgagcgt cgaaacggat
      661 ataacggcca ccgccctgac ggtccgctcg catatcggcg agttggcgct ggatagtcgg
      721 tatcacgacg acggcaaaga atacgatgtg cggatccata agtacgtgct gccgaatgcc
      781 atcgaccagg gcgatgaagc tgccgactat ttctcagggc tgctggagcg gccggtgcgc
      841 ctgttgcgag ccaaccgggc cgttatgcga cgcatttcgc cggaacggcg acgtccggac
      901 gcctccaaca gtgtggcggc ggccgatgca taccctttct tactcaccaa ccaggcgacg
      961 ctgaacgcct tgcaccgggc cgccggcatg gagctgggta ctattccgat gaaccgcttc
     1021 cgccccaata tcgtcataga ggggagcggg gtaagggcca tgggcgagga tctcatgcgc
     1081 gaggtggtca tcggtggact ttatgctcac gtggtgaagc cctgtgaccg ctgtcctata
     1141 ccgaacatcg accagcaaac aggcagccgg gatgttccgg cgggactcaa gttactgaag
     1201 tatcatcgag gccggttacc cggcgatgaa aaagcacggc tatttttcgg ccagaatctg
     1261 aacccggtgt ttcggagcgg cgttaccgtt catcggggtg acaaagtgcg ggtcatccgc
     1321 caagagaaaa caccgaactt cgcgcctgta cgctagccgc cacggtgcta caattgaggt
     1381 atgaaaccgt tccgtcgggt agaaccgacc gtcgtgcaaa aagtggggca ccggtcggtc
     1441 gtgaccaagc atttcgtcat gccggatggc agcacctatg actttcagac attcaacgcc
     1501 gagggcaccg agttcgccgg cgtggtggcc ctcacgcccg accggcaggt tataaccgca
     1561 tatcagtatc gccccgggcc cgaagctttc atgtatgaaa tacctggtgg agggacggat
     1621 gacggtgacg acggcgatat ggccgtcacc gcaacacgcg agctgcgcga agaaaccggc
     1681 tataaacctg gcacgctgac gtacctgggc aaggtgtaca aagacgccta caacaacgcc
     1741 acctggcatt actttctggc ggaagactgc gtgctggacg gcgaacaaga gctcgatgcc
     1801 accgaagccg tggaggtgcg gctggtgccc atcgaccggt tcctggacta cgcccgcaac
     1861 gggcagatga ccgataccga agccgtcctg ctggcctacg accggctgat ggcgctgggc
     1921 tcagttcgcg tttaagtgtc atactggtag aacggtaaac gagggaggcc gatgccgaga
     1981 ggtgcacaaa acgcccggga gcataacgtc gacgagtttc ggcattgtat cgatgcggtc
     2041 atcggccagg tgccgccttt gccggaagcc gagcaagtcg gcatcgcccc catgcggcgg
     2101 caggaggacg ccacctacat ctctgccgac cggcgcgaag gcaccccggt ggttatcaat
     2161 cgattggaat cggcgcataa atgggcgctg ctgacgcgca ccagcttcac aatgtacacc
     2221 ggttcgctgg caatcgtatc gtacctggag cggccgcgcg acctggaccg catatggctg
     2281 attgatgatt accgtggagc ggccaggatc cggaccatca aacggttaaa gacggaaccg
     2341 gagctgcaga tggcggcttc gctggccttg tcgcccgtcg gttttaagcg gctatggacc
     2401 gaactggggg cattgaccca gagcgaaagc gactttgtgt acgacaagtt ccgtcaaccg
     2461 ggtgatgaga cggctgcgct gcaggaattc agtgcgttgg agccggaatt aggcgataat
     2521 ggttggtaag cctaaacacc aaggagaaac gatgccccaa gtgaccaaag ccgtcatagc
     2581 cgccgccgga ttcggcacgc gctttttgcc ccagaccaag gccatgccca aggagatgct
     2641 gccgctcata gataagccca ttatccagta catcgtcgaa gatctggtgg cggccggcat
     2701 ccaggacatc atcattgtcg gcagcagcaa caaacgggcg atagaagacc attttgacgt
     2761 gccgaacgag gatctgctgg ccaacctgcg cgccggcggc cccaagaagc agccgttcat
     2821 cgacgccatc gaggccgtcg ccaacctggc gaacttcatc tacgtgcggc agaagggccc
     2881 ctacggcacg gccacgccac ttatgaacgc tgcccacctc ataggcaatg aaccgttcat
     2941 ctatgccttt gccgacgacc tgaccgtcgc cgaaccgaat gccttccagc agctgatcgc
     3001 cctgcacgag gagcttggcg gcggcattct gccgtgcatc cgggctaccg aagacgtcga
     3061 ctacgagcgt tacggtatca tcggcggtga agtggtgcgc gacggcgtca tgcgcatggc
     3121 gtccatcatc gaaaagcccg gtaaagccgg cgcgccttcg gatttcgcgg ccgtcggcgg
     3181 gtatctgttc acgccggata tcttcgacta tctggagcag ggcctgcgcg acctgcccgc
     3241 cggcaaggag ttttatgtga cagacagcat aatcgagccg atgttacggg acggcaagcc
     3301 gtacttcggc gccgtcattc agaacggcca gcgctacgac accggcgaca agctggagta
     3361 cctcaagacg gtcatcgact tcggcctggc ccacccggaa ctcggcgcgc ccttgcgcga
     3421 ctttatcgac cagcgtctgc aagactaggc cggccgagct cctgaatatc cagcgcacgg
     3481 tccagcagcc gcgccagcgc attcagccct atatcgatgc cgctttgctc acgtggcgca
     3541 gccagccgct ctggcgaggc gttgaacagc tcagccaatg gttcgagtga ggtgctgggg
     3601 tcagacctaa agagtggcag ccgatgcctg actgggcaac cgctgctggt g
//
LOCUS       DASSFD010000193         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1206696, whole genome shotgun sequence.
ACCESSION   DASSFD010000193 DASSFD010000000
VERSION     DASSFD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206696"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFH39_04705"
     CDS             <1..938
                     /locus_tag="VFH39_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET6925105.1"
                     /translation="NEGMTTIYYSRARERALQAVDEPRAGSWIVSVAPDEAELDALAE
                     HYGLDRDNLTDAVDIYEAPRVEIDDGAVYIYTRYCYPEGTEIATEPLLIIYTADYLIC
                     VQRASTPILERLTTDVIDVVTTQKTKTLLQIFAEINRSYEAQARKVSRQILQIRAHMR
                     QARITTREFVGIIELEEDLNEFLSALQPQALLFSSLLSGKYLRLYEEDRDIIEDIERS
                     TSELITQVQGRLRTLVNMRQAYDAIATNNLNNTFKRLTSIAIFLAIPTVISGMWGENL
                     GVPFKDTTYGFWIITGFIVACTAGAIWIFRRKRWL"
     gene            complement(940..1641)
                     /locus_tag="VFH39_04710"
     CDS             complement(940..1641)
                     /locus_tag="VFH39_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HET6925106.1"
                     /translation="MAAADSYVPPAVIVARANHTVSAGSETKPAPVTVQPAHAVPVPA
                     CTPVTVAMPGAVALQTGTRGVQIVADPVYYYKVYGTTVHQAAAETAACAPLAQGTREY
                     TGYTAYTLNWRYTFTPTAGGSCAIGDAAVGLHIQTVVPDWADAQTAAVADQAAWRQFA
                     QNLMNHEQGHGSLDRHFADELYAKLQTLSATDCATLSTQAASIARQYVADVAAANTNY
                     DQMTDHGATQGAILP"
     gene            1874..2563
                     /locus_tag="VFH39_04715"
     CDS             1874..2563
                     /locus_tag="VFH39_04715"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET6925107.1"
                     /translation="MKFIRTAGWEEGTKALGKRLVKELQSKQVLWLVCGGSNIAAAVQ
                     VMQSLPDELTEHLSIFLTDERFGEIGHRHSNAAQLLDAGFQTKQAVFVPVLQPGFSLS
                     ETQERYGQALASAVEHADIVIGQFGIGPDGHIAGILPHSPATTADGWMAAYEAPPYTR
                     VTMTFPALRHIDVAFAMAFGDDKRGALSRLETEELPLDTQPSQILKELPEAYVYNDQV
                     GKESKTGKEPA"
     gene            2560..>2813
                     /locus_tag="VFH39_04720"
     CDS             2560..>2813
                     /locus_tag="VFH39_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6925108.1"
                     /translation="MKIAVHGLGRMGMQIARKLAESEHTLIAHNRSKDKIDTAATYGA
                     VKAYEKQDVLDAFAGEQVVLWLMLPADIVDEEVEQWLQLLP"
BASE COUNT          625 a          817 c          834 g          537 t
ORIGIN      
        1 acaatgaggg catgacgacg atatattaca gcagggcccg cgaacgggca ttgcaggcgg
       61 ttgatgagcc gcgggccggt tcgtggattg tcagcgtcgc tccggacgaa gccgagctgg
      121 acgctctggc cgaacactac gggctggacc gcgacaacct taccgacgcc gttgacatct
      181 acgaggcgcc ccgtgtcgaa atcgacgacg gcgccgtgta catctacacg cgctactgct
      241 acccggaggg tacagagatt gcgacggagc cgctgcttat catctatacc gccgactacc
      301 tcatttgcgt gcagcgggcc agcaccccca tacttgagcg gctaaccacc gatgtcatag
      361 acgtcgtgac aacccagaag accaaaacgt tattgcagat ttttgccgag ataaatcgtt
      421 cgtacgaagc acaggcccgc aaagtctccc ggcagatcct gcagatccgt gcccacatgc
      481 gccaggcacg catcaccacc cgcgagttcg tcggtatcat cgagctcgag gaggatttga
      541 atgaattctt gagcgccctg cagccccagg cactgctgtt cagctcactt ttgagcggca
      601 aatatctgcg tctgtacgaa gaagaccgcg acattatcga ggacatcgaa cgcagcacca
      661 gcgagctcat cacgcaggtg cagggccggt tgcggacgct ggtgaacatg cgccaggcat
      721 acgatgccat cgccaccaac aacctgaaca acaccttcaa gcgcctgacg tccatcgcta
      781 ttttcttggc gattccgacc gtcatcagcg gcatgtgggg agagaacttg ggcgtaccgt
      841 tcaaagatac cacttacgga ttctggatca ttaccggttt tatcgtcgcc tgtacggcgg
      901 gggcgatctg gattttccga cgcaagcgct ggttataagc tagggtagga tcgcgccctg
      961 ggtggcgccg tggtcggtca tttgatcgta gttggtgttg gcagccgcga cgtcggcgac
     1021 atattgccga gcaatcgaag cggcttgggt ggaaagcgta gcgcagtccg tggccgagag
     1081 tgtctgtagt ttggcataga gctcatccgc aaagtgacgg tccaatgagc catgcccctg
     1141 ttcgtgattc atgaggtttt gggcaaactg gcgccacgca gcttgatcgg ccaccgcggc
     1201 ggtctgggca tcggcccaat caggtaccac cgtctggatg tgcaacccga cggcagcatc
     1261 accgatagcg cagctgccac cggcagtcgg ggtgaacgta taacgccagt tcagcgtgta
     1321 ggccgtgtag ccggtatact cgcgggttcc ctgggcaagc ggcgcgcaag ccgcggtctc
     1381 cgctgccgcc tgatgaacgg tagtaccgta taccttgtag taatacaccg ggtcagcgac
     1441 gatctgcacc ccgcgcgtgc cggtctgcag ggcgaccgcg ccgggcatgg cgaccgttac
     1501 gggcgtgcag gcaggtaccg gcacggcgtg agccggttga acggtaacag gagcaggctt
     1561 cgtttcactt ccggccgata ccgtgtgatt cgcacgggct acgatgaccg ccggcggaac
     1621 atacgagtcg gctgcggcca tagcggcgta caagcggtcc cggacggcaa gtacggagcc
     1681 gctgattagc agactgccta tcagcaggaa acatatccgg acaaatgggt tagcaggtgt
     1741 aaatactctc ataaaaactc aataaaaaaa cacagcgggc gtagccctct gtgcgcatta
     1801 atactattat ataacagtta gggttacttt acaatatacc ggtgaacgcc gaccggggtg
     1861 tactatggca gtaatgaagt tcattcgtac ggcggggtgg gaagagggaa ccaaggcttt
     1921 gggcaagcgg ctcgtgaagg agctgcagtc taaacaggta ctgtggctgg tctgcggcgg
     1981 ctcgaatatc gcggcggccg tgcaggtcat gcaatcactc cccgacgagc tcactgaaca
     2041 cttaagcatc ttcctgaccg atgagcgctt cggcgagata ggccatcgcc acagcaacgc
     2101 cgcccagctg ctggatgccg gcttccagac aaagcaggca gtcttcgttc cggttttgca
     2161 gcccggcttc agcctgtccg agactcagga acgttacggt caggccctgg cttcggccgt
     2221 ggagcatgcc gacatcgtca tcggccagtt cggcatcggc cctgacgggc acatcgccgg
     2281 tattttgccg cactcgccgg ccaccaccgc ggatggttgg atggcggcct acgaggcacc
     2341 tccgtatacg cgtgtcacta tgacgtttcc cgctctccga cacatcgatg tcgcctttgc
     2401 catggcgttc ggcgatgaca aacgcggcgc gctcagcaga ctcgaaaccg aagagttgcc
     2461 cctggacacc cagccgtcgc aaatcctcaa ggaattacca gaagcctacg tatataatga
     2521 ccaggtaggc aaagaatcga aaacagggaa ggagcccgca tgaaaatagc cgtccacggc
     2581 ctcggccgca tgggcatgca gattgcccgc aaattagccg agagcgaaca tacgctcatc
     2641 gcacacaacc gcagcaagga caagatcgat accgccgcga cctacggtgc cgtaaaagcc
     2701 tacgagaaac aggacgtgct cgacgccttc gctggcgagc aagtcgtttt gtggttgatg
     2761 ttgcctgccg acatcgtcga cgaagaagtc gagcagtggc tgcaactgtt gcc
//
LOCUS       DASSFD010000194         3963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_104415, whole genome shotgun sequence.
ACCESSION   DASSFD010000194 DASSFD010000000
VERSION     DASSFD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104415"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VFH39_04725"
     CDS             <1..1019
                     /locus_tag="VFH39_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6925109.1"
                     /translation="SKIVDPTEGSFETTGRVASLLEVGTGFHPELTGRENLYFNGSIL
                     GMTQKEIGARFDEIVDFAEIGKFIDTPVKFYSSGMSVRLGFAIAAHLDPEVLIIDEAL
                     AVGDVAFQQKCIEKMRNSARSGKTVLFVSHSMNIIEDLCDRVMYLKNGKLVDIGDPDR
                     VMSAYLGTTKKDVGTSWQIANDYVDKPDNPVRPLQMKLLHEDGKPIGPFINKGKPVVV
                     DFEVDIKNLTSDLSVGVSVFNEVGVHMYRTATTDYPDQNLKLSPGTNHLQVVLPTSEL
                     LDGPYIVSLDCDIYKKQWAHNPYFSEMRTRFLVKADASDPVKTWEPSREGAYKPHLSW
                     QKVS"
     gene            1021..2157
                     /locus_tag="VFH39_04730"
     CDS             1021..2157
                     /locus_tag="VFH39_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6925110.1"
                     /translation="MARRRIRVLFDANPLLVQKTGVGHYTYRLIRALAGRDDLELIGY
                     YSGALGDAALPEGVSYHRLPVPKKIINQLRRMRLLPPIELLTGRRADFLFFPDFLGLP
                     SLFRTPRACVIHDMTFLDLPEYVSRRNQRDLARFVPREVARSRFVVTVSEFSKQRLRE
                     AYGLKAEGILVTPIPPDNPLPADTKIDPETLQQLGIDGKYILTLGTIEPRKNLVSMLE
                     TYVALPPQVREEYAFVVAGKVGWNCEAEIAKLQELKDAGYRICQIGYVDDRQRAVLYR
                     YASLFTMASRYEGFGMQALEAMSYGTACAVSDIPVFREVSGDAAVYFEGGRPEAITKA
                     WLRLLQDDAYRKELVSAGLQRVAGYDWSTVASSLHDRIIGSLGN"
     gene            complement(2210..>3963)
                     /locus_tag="VFH39_04735"
     CDS             complement(2210..>3963)
                     /locus_tag="VFH39_04735"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HET6925111.1"
                     /translation="TWHGTTQETRRKHKSMNEPHGGKLIDLMVSPEKAKTYKKDIQSL
                     PRWTLTMRQACDFELLSNGGFSPLTGFMDEPTYLSVCKDMRLPGSNLVWPMPITLDVT
                     AEFAKSLKPAQKVVLEDAEGVALGILTVKDIYKPDKKAEAEWVFKTTDTAHPGVNYLF
                     NQAGEYYLGGDIEALALPHHYDFVDLRLTPKELRERFKKLGWQKIVAFQTRNPMHRAH
                     VELTLRACADNAMNLLINPTVGMTKPGDVDHYTRVRVYKHILDKYPPSTATLSLLPLA
                     MRMGGPREALWHSIIRKNYGATHFIIGRDHAGPGKDSNGNDIYGPYESQQLAEKYAPE
                     IGVEIVPFQMMVYVPKLDRYKPVDELAEGEEHQTLSGTELRALLNTGKELPSWFTYPE
                     VAAELRRSYKPRKERGFVVFFTGLSGSGKSTLAKGLLSRLHETGGRTITLLDGDEVRR
                     HLSSELTFSKEHRDLNIKRIGYVAAEIAKHGGIAICAPIAPYESLRQEVRRMAESCGA
                     GFIEVYVNTPLEVCEQRDRKGLYAAARAGKIKNFTGISDPYEPPKNPEFVADTTTSTR
                     EELVQSILLAIENAGYL"
BASE COUNT          771 a         1115 c         1201 g          876 t
ORIGIN      
        1 tgtcgaagat tgtagaccca actgagggca gcttcgaaac caccggccgc gttgccagcc
       61 tgctggaggt cggtaccggc ttccatccgg aactgaccgg ccgcgaaaac ctgtacttca
      121 acggctccat cctgggtatg acgcagaaag aaatcggcgc tcgttttgac gagatcgttg
      181 actttgccga aatcggtaaa ttcatcgata cgccggtgaa gttctacagc tccggtatga
      241 gcgtccggct tggcttcgct atcgccgcac accttgaccc ggaagtgctg atcatcgacg
      301 aagccctggc tgtcggtgac gtagcgttcc agcagaagtg tatcgagaag atgcgcaatt
      361 cagcgcgctc cggcaagacg gtgctgttcg tgtcacacag catgaacatc atcgaggatc
      421 tgtgcgatcg ggttatgtac ctgaagaacg gcaagctggt ggacatcggc gaccccgacc
      481 gcgtgatgag cgcctacctg ggcaccacca agaaagatgt gggcaccagt tggcagatcg
      541 cgaacgatta tgtcgacaag cccgacaacc cggttcgccc cctgcaaatg aagcttttgc
      601 atgaggatgg taaacccatc gggcctttca taaacaaagg caaaccggtg gtcgtcgact
      661 tcgaggtcga catcaagaac ctgacgagtg acctatcggt cggggtgagc gtcttcaacg
      721 aagtcggtgt acacatgtac cgcaccgcca ccaccgatta cccggatcag aaccttaaac
      781 tgagtcccgg caccaatcat ttgcaggttg tgctgccgac ttcggagctg ctggacgggc
      841 cttacatcgt gtccctggac tgtgacattt acaaaaaaca gtgggcgcac aacccttact
      901 tctctgagat gcgcacccgt ttcctggtta aagcagacgc gtctgatcca gtcaaaacat
      961 gggaaccaag ccgtgaaggt gcctataagc cgcacctgag ctggcagaaa gtgagttagc
     1021 atggccaggc gccgtatacg ggtgctcttc gatgccaatc cgctcctggt gcaaaagaca
     1081 ggtgttggcc attacacgta tcgcttgata cgtgcacttg ccggccgcga cgacctagaa
     1141 ctcataggtt actactcggg tgcgctcggc gatgccgctt tgccggaagg cgtctcctac
     1201 catcgccttc cggtaccgaa aaaaattatt aaccagctcc ggcgcatgcg cttactacca
     1261 ccgattgagt tactgacggg ccggcgagct gacttcctgt tcttcccaga ttttctcggg
     1321 ctacccagcc tctttcgcac tccccgggct tgcgtaatcc atgacatgac attccttgat
     1381 ttgccagagt acgtctcgcg ccgtaatcag cgtgacctgg cacgtttcgt gccgcgagaa
     1441 gttgcgcgct cccgtttcgt tgtgacggtc tcggagttca gtaagcagcg gcttcgtgaa
     1501 gcctacggat tgaaggcgga gggcatttta gtgacaccga ttccgccgga caatcctttg
     1561 ccggcagata cgaaaatcga cccggaaacc ttgcagcaac tcggcataga tggtaagtac
     1621 atcctcacgc tcggcaccat tgaaccacgc aagaacctcg tgtcgatgct cgagacatat
     1681 gtggctctgc ccccacaagt acgtgaggaa tatgccttcg tggtcgccgg taaggttggc
     1741 tggaactgcg aagcggaaat cgccaagtta caggaactta aggatgccgg ttaccggata
     1801 tgtcagatcg ggtatgtcga cgaccgtcag cgggcagtcc tctaccgcta cgcttctctc
     1861 ttcacgatgg ctagccgcta cgaaggattt ggtatgcagg cgctggaggc catgagctac
     1921 ggcacggcgt gcgccgtcag cgatataccg gtcttccgcg aagtttccgg cgacgccgcc
     1981 gtatatttcg aaggtgggcg accagaggct attacgaaag cctggctcag gctgcttcag
     2041 gatgatgcgt atcgcaaaga gctggtatct gccggactcc agcgtgttgc tggctacgac
     2101 tggagcacgg tggcttccag tctgcacgat cgcatcatag gatcattggg aaattaaagc
     2161 tctctctggg cgtggtgatg gatatgggcc gttggggtta agctaggttc tacaaatacc
     2221 cggcgttttc gatggccagg aggatgctct ggactagttc ttcgcgggtg ctggtggtgg
     2281 tgtcggccac gaattccggg ttcttgggcg gttcgtacgg gtcggagatg ccggtgaagt
     2341 tcttgatttt gccggcgcgg gcagcagcgt acaggccttt gcggtcgcgc tgttcgcaca
     2401 cctctagcgg cgtattcaca tagacttcaa tgaagccggc cccgcaggat tcggccatgc
     2461 ggcggacctc ctggcgcagc gactcgtagg gtgcaattgg cgcacaaatg gcgatgccgc
     2521 cgtgtttggc gatctcggcc gccacgtagc cgatgcgctt gatgttcagg tcgcggtgct
     2581 ccttgctgaa cgtcagctcg ctggagaggt ggcggcggac ttcgtcgccg tccagcaagg
     2641 tgatggtacg gccgcctgtc tcgtgcaggc gcgacagcag gcctttggcc agcgtcgact
     2701 taccggaacc ggacagccct gtgaagaaca cgacgaagcc gcgctctttg cggggcttgt
     2761 aggaacggcg cagttcggcg gcgacttccg ggtaggtgaa ccagctgggc aactctttgc
     2821 cggtgttcag cagtgcccgc agctcggtac cggacagcgt ctggtgctcc tcgccctcgg
     2881 ccagctcgtc gaccggcttg tagcggtcca gcttgggcac gtagaccatc atctggaacg
     2941 gtacgatttc gacgccgatt tccggcgcgt acttctcggc cagctgctgt gactcgtacg
     3001 gcccgtaaat gtcgttgccg ttgctgtcct tgcccggacc ggcgtggtcg cggccaatga
     3061 tgaagtgcgt ggcgccgtag ttcttgcgga tgatggagtg ccacagtgcc tcgcgcgggc
     3121 cgcccatgcg catggccagc ggcagcagcg acaacgtggc ggtggaaggc gggtatttat
     3181 cgaggatgtg cttgtagacc cgcacccggg tgtagtggtc gacgtcaccc ggtttggtca
     3241 tgccgaccgt cgggttgatg agcaggttca tggcattgtc ggcgcaggca cgtagcgtca
     3301 gctccacatg cgcccggtgc atcggattgc gggtctggaa agcgacgatc ttctgccaac
     3361 cgagcttctt gaagcgctct cgcagctcct tcggtgtcag ccgcaggtcg acgaagtcgt
     3421 agtggtgcgg cagggccagg gcctcgatat cgccgcccag gtagtattcg ccagcctgat
     3481 tgaacagata attgacaccc gggtgggcgg tgtcggtcgt cttgaagacc cactcggcct
     3541 cggccttttt gtcgggctta tagatgtctt tgacggtcag gataccgaga gcgacacctt
     3601 cggcgtcctc caggacgact ttctgcgcgg gcttgagcga cttggcgaac tcagccgtca
     3661 cgtccagggt gatcggcatc ggccacacca gattactgcc cggcaggcgc atatctttgc
     3721 agaccgacag gtaggtcggc tcgtccatga aaccggtcag cggactgaaa ccgccgttac
     3781 tgagcagttc gaagtcgcag gcttgacgca tggtgagggt ccagcggggc aatgactgaa
     3841 tgtctttctt gtatgttttg gccttttcgg gcgagaccat caggtcgatc agtttgccac
     3901 cgtgcggttc gttcatgctc ttgtgtttcc tccttgtttc ctgcgtagtg ccgtgccaag
     3961 tgt
//
LOCUS       DASSFD010000195         8039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1740674, whole genome shotgun sequence.
ACCESSION   DASSFD010000195 DASSFD010000000
VERSION     DASSFD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740674"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFH39_04740"
     CDS             <1..182
                     /locus_tag="VFH39_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925112.1"
                     /translation="LAMAGAWAAMFSYLLAANFDGVPGALLRLLGVCLVVITTGLALV
                     AVADYAAQYRALHAK"
     gene            complement(175..732)
                     /locus_tag="VFH39_04745"
     CDS             complement(175..732)
                     /locus_tag="VFH39_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HET6925113.1"
                     /translation="MLHNIALAFWFFLPPALANATPIGVAALPYVKRWDAPMDFGKSF
                     QGWRILGPHKTWRGLIAGMLSSTIALWLQQLAYDHTGWGASIGHGVPYNTLPTLVLGP
                     LFGLGALGGDAIKSFFKRRRGTPSGATWLPFDQLDYVVGGTVVSLPFVVLAWPVYVWI
                     LVLWFGIHFIASYLGWKAGLKQQPI"
     gene            1455..1799
                     /locus_tag="VFH39_04750"
     CDS             1455..1799
                     /locus_tag="VFH39_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET6925114.1"
                     /translation="MSSYDNTERNALVAFGAIFGMAAGVGLGMLLAPRSGQETRRQIS
                     DKARTAQDKAKQQFASKRDMAAAKLSQSLDKTKDMANKMADKSKDVADKTTARAKDAA
                     QQARSETDTERS"
     gene            1826..2041
                     /locus_tag="VFH39_04755"
     CDS             1826..2041
                     /locus_tag="VFH39_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925115.1"
                     /translation="MTGLQTAFYIVALVFMGINLLLIIALLSAVLVIKAKVNKLHDAV
                     DEKVSQVKDIGAKAGLVFRTVKHFAKR"
     gene            complement(2038..2274)
                     /locus_tag="VFH39_04760"
     CDS             complement(2038..2274)
                     /locus_tag="VFH39_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925116.1"
                     /translation="MWHLHFWHWLMDFTGVNNGDDSFGTHMYNFWSGFGGNISVLALL
                     GTILGVYRHNLKRFDKLNPLRIVRKKEGDDAADS"
     gene            complement(2344..2799)
                     /locus_tag="VFH39_04765"
     CDS             complement(2344..2799)
                     /locus_tag="VFH39_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925117.1"
                     /translation="MSEIASREHQVAYHEGYETAERTSGWVGWVGFGGFMMILAGILH
                     VLDGLVGLYRSSFYLVTNHSTELLLFPNVRAWAWVNLIAGAVVILAGVALFSGAMWAR
                     VLAVILSIIVVAGNVLAITLYPLWSIIAIALAILVIYAVTVHGGELRHE"
     gene            complement(3232..3308)
                     /locus_tag="VFH39_04770"
     tRNA            complement(3232..3308)
                     /locus_tag="VFH39_04770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3272..3274),aa:Met,seq:cat)
     gene            complement(3500..4264)
                     /locus_tag="VFH39_04775"
     CDS             complement(3500..4264)
                     /locus_tag="VFH39_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6925118.1"
                     /translation="MDLKDILLFGAGLAVGSMNAIAGGGMLMGFPVLLATGLPALIAN
                     GTGNVVIFPGQLASAVGYWKYLRKTPWKYAWLLAPWAVGAVIGALLLRRTPSMTFERY
                     VPGLILFAVLLFAFQPFLHHYLHKHLKSRSKRVKPLVIIGIALFPVAIYGGYFGAGVG
                     FIMLAFLGFTKLHDAHQINAMKNVAATVSAAICMVILSHGHFINWHAGLVMAAGSTIG
                     GYAGARYAQSISSHAIRVVVIAIGIVTVTYLGFRSY"
     gene            complement(4301..4732)
                     /locus_tag="VFH39_04780"
     CDS             complement(4301..4732)
                     /locus_tag="VFH39_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6925119.1"
                     /translation="MSSDRPKVGIGLLLIKDGKYILLGKRKSSHGDGEYAGVGGHMEH
                     LETVNETIMRELQEEIGDDIRISTPQFLCLSNIRDYAPKHYIDVGLVAEWISGEPKVM
                     EPDKIESWDWYPLDAIPEPLFASCKHYLAAYRTGTVFFENS"
     gene            complement(4729..5247)
                     /locus_tag="VFH39_04785"
     CDS             complement(4729..5247)
                     /locus_tag="VFH39_04785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6925120.1"
                     /translation="MKKLYFVRHGQSQANVDETFAGQSDTPLTSLGREQAKTAGKAAR
                     ELHIDRIVSSPLSRAFDTAVIIAQTIGYPVSNIDINDLFKERNYGSLQGKAWGSQGEE
                     DINGIAGVEPIESLLQRGRQAYQYLHDLPDDTVLVVAHGTFWRALRAAMHPDIPFIAG
                     TEPRNAVVEELA"
     gene            complement(5244..5519)
                     /locus_tag="VFH39_04790"
     CDS             complement(5244..5519)
                     /locus_tag="VFH39_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925121.1"
                     /translation="MPTNNSLLKRIEQLEARNRRVEADKGWETSWTRRLAIMFLTYWI
                     VVAYLQFVVHIDPWINALVPVIGFFLSGLTLTLLKRQWIANRIRSRR"
     gene            5624..6490
                     /gene="tilS"
                     /locus_tag="VFH39_04795"
     CDS             5624..6490
                     /gene="tilS"
                     /locus_tag="VFH39_04795"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET6925122.1"
                     /translation="MKLDMPPGNYVVAVSGGVDSVVLLDLLRYHSGLKLTVAHFDHGI
                     RSDSRLDRIFVQQLAHRYQLPFVFDAVTLGPGASEAAARKARYAFLKRAQQAAGAQAI
                     ITAHHQDDMLETAVLNLLRGTGRKGLSSLTTGEGVIRPLLEVPKSELLDYARRHRLSW
                     REDSTNNDTNYLRNHIRHVMLPSWREADKQRLLGIIRHMREVNGSLDAVLDDSLTPQL
                     KREWFVLLPHNVAREVMASWLRSADIREFDRRTLERLVVAGKVAEAGKAIDVVKGRRM
                     RVGRDHLALEVH"
     gene            6580..>8039
                     /gene="ftsH"
                     /locus_tag="VFH39_04800"
     CDS             6580..>8039
                     /gene="ftsH"
                     /locus_tag="VFH39_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6925123.1"
                     /translation="MDKSPLKNNKQKPGGSRRGLKNAGFIALIILAALIILAATSQPS
                     NLKTIPVTTAVQQANAGDYSKITVNGNQLTITKKGDNHPTLKSYLEPNATMKDEGFNY
                     SKVEIDPQPESNSSSVWLTLASTILPVILIGGLLFFMLRSAQGQGNQAMSFGKSRARL
                     YGNEKDKVTFDNIAGSGEAKQDLEEVVEFLKYPKKYAHMGARIPKGVLLVGPPGTGKT
                     MLARAVAGEANVPFFSISGSEFVEMFVGVGASRVRDLFAKAKKNAPCIIFVDEIDAVG
                     RRRGSGMGGGHDEREQTLNQILVEMDGFEQGQSVIVLAATNRADVLDPALLRPGRFDR
                     RVDIGLPDRKDREAILKVHFAKKPLAKAVDLDALAAKSAGSSGADLANIANESAIIAA
                     RNNHQEISADDVTEAFERVAIGAERKSKVMTEKDKLTTAYHESGHALVGHVLPDSDPV
                     HKVTIIPRGGTGGVTWFLPPEDRVYTSLVEFKDVLAR"
BASE COUNT         1793 a         2281 c         2335 g         1630 t
ORIGIN      
        1 agctggccat ggccggagca tgggcggcga tgttcagtta cctgctcgcc gccaacttcg
       61 acggcgtccc cggggcatta ctccgcttgc tgggcgtttg tttggtagtg attacgactg
      121 gcttggcgct ggtggcggta gctgactacg ccgctcaata ccgggcgcta cacgctaaat
      181 aggctgttgc ttgagtcccg ccttccaacc caggtagctg gcgatgaagt ggatgccgaa
      241 ccacagcacc aggatccaga cgtagaccgg ccaagcgagc acgacgaacg gcaggctgac
      301 taccgtacca ccgaccacgt agtcgagctg gtcgaagggc agccaggtgg ccccggacgg
      361 tgtaccgcgc cggcgtttga agaagctctt gatagcatcg ccccccagcg cgcccagccc
      421 gaacaggggt ccgagtacca acgtaggcag cgtattgtag ggcaccccgt ggccgatgga
      481 cgctccccag ccggtatgat cgtacgccaa ctgttgcagc cagagcgcga tagtcgagga
      541 cagcatgccg gcaatcaggc cgcgccaggt tttgtgcggt cctaagatac gccagccttg
      601 gaagcttttg ccgaaatcca tcggagcgtc ccagcgcttc acatacggca gggcagcgac
      661 tccgatgggc gtcgcgttgg cgagagcagg gggcagaaag aaccagaacg ccagcgctat
      721 attgtgtaac attgcttcat catagctggc atagccctaa aaaccaatca ttgtaaaaac
      781 ctgcacacta cattgacaaa ataacaagca taagctataa taatagatgt tatggccatt
      841 aacaaacatc tacaaaaata aaagaaagaa tgaagtttta gttatgaaga ccaaaagcgg
      901 attggcgttt gaattcgtca ccactaccaa tcaggatatc ctgcggttag actcgctgct
      961 caggaagctg taagatgctc atccaggata tattgccacc gcagcaggtg agggacatcc
     1021 gggagttatt cccggccgac ccgccgaaac gcggtaagca caagagccgc aagatcgcgg
     1081 ttcactaaac ccgaagagcc ctgcggatag gcagggctct tttagtaata tttctcatag
     1141 ttagcactag cgggacgtga gatgctaagt gccaagcaag gagggcttag cacatggcac
     1201 gtaacgagaa tatagagaat agtgacatcg ccaagcagta cgaatttacc cgcagccgcg
     1261 gcaccggcga attctggcgt gagttcatca tttattagcg gttgataagt ttttgacacc
     1321 cggtcatccc gtatggccgg gtttcattat gtaattgaag ttgcactacc ctacagtaag
     1381 gagtggatta tcttactgct gaatgcaacg tagcgtacgt atagtaaacc taccatttaa
     1441 cgaaggaggc ttatatgtcc agctatgaca acaccgagcg taacgcgctg gtcgcattcg
     1501 gcgccatatt cgggatggcc gcaggggtag gcctcggcat gctgctggcg ccacggagcg
     1561 ggcaggagac caggcgacag atcagcgaca aggctcgcac ggcccaagac aaggccaagc
     1621 aacagttcgc ttccaagcgc gacatggccg cggccaaact ctcgcagagc ctggataaga
     1681 caaaagacat ggctaataag atggccgata aatccaaaga cgtagccgac aagacgactg
     1741 cccgggcaaa ggacgctgcc cagcaggcga ggtccgaaac tgataccgag cgttcctaac
     1801 taaccaagct aaggagtcta ccgttgtgac aggattacaa accgcattct acatagtcgc
     1861 gctggtgttc atgggaatca acctgctgct gataatcgcc ctgctgtcgg ccgtgctggt
     1921 catcaaggcc aaggtaaaca agctgcatga tgccgtcgac gaaaaggtga gccaggtcaa
     1981 agatatcggt gcgaaagccg ggctggtttt taggaccgtc aagcacttcg cgaagcgcta
     2041 gctgtcagct gcgtcgtcgc cttccttttt gcgcactatg cgtaaagggt tcagcttatc
     2101 gaagcgtttg aggttatggc ggtacacccc caggatggtg ccgagcagtg ccagcacgct
     2161 gatgttgccc ccgaagccgg accagaaatt gtacatgtgc gtgccgaacg aatcgtcacc
     2221 gttgttcacg ccggtgaagt ccataagcca gtgccagaag tgcaggtgcc acatagggtt
     2281 cgcttaagta taacaaaggc ctctcgatgc gtgagaggcc tttgtggtac gcgccgccgg
     2341 tcgctactcg tgacgcagct cgccgccgtg aacggtcacg gcgtatatca ccaggattgc
     2401 caacgcgatg gcgataatcg accacagcgg gtacaacgtt atcgccaaga cattaccggc
     2461 caccacgatg atcgacagga tcacggccag tacgcgggcc cacatggcgc cgctgaatag
     2521 cgccaccccg gccaggatta cgactgcgcc ggctatgagg ttgacccatg cccaggcgcg
     2581 gacgttcggg aacagtaaca gctcggtcga gtggttggtg accaggtaaa acgagctgcg
     2641 gtacagcccg accagcccgt ccaggacatg caggatgcca gccaggatca tcatgaagcc
     2701 accgaagccg acccagccga cccagccgct ggtgcgctca gccgtctcgt agccttcgtg
     2761 atacgcgacc tggtgttcgc gtgatgcgat ttcgctcata tgtatacctc catttattcg
     2821 tagttcaatg ttctgtgctt attggcgcga ttcctgcccg aagagaagcc gcgatgtctg
     2881 tttttagtgt cgcgtccata ctccctcctc cccgcttaac tgctttcaac tgtagtcctg
     2941 cttccgactg acttccatga cggccatcac aagctggccg tgcttgtccg caaaacaaaa
     3001 agccctttgc aggtcgtgcg aaaggcttga ttgatacttt tggtagcagg ggcgagactt
     3061 gaacgaacgc cggatggcag actagctgcg ctattccacc atccgctaca cgctgtcaaa
     3121 tggggcattt gacagccttc tgctcccagc tcgaacaagt tcgagaggcc gcaagttcaa
     3181 atctgccgcc ttgccaagca aagaccccat cgcctggacg gggtcttaac ttggtagcag
     3241 gggcgagact tgaactcgcg accccaggct tatgagtcct gtgctctaac cagctgagct
     3301 accctgcctt cgtcggaact tacggctcag tctcgttttg tcgcttaagc gacaaaagct
     3361 tcgacccttg cctgtggccc tcctctcaaa atcgcattca gtcgccggag gctccttgga
     3421 ttgcgatttt ggttttcgct gcagaattgc agtttaacaa cgggagattg tagcaggttt
     3481 tgcctctgtt ggccagtggt tagtaactgc ggaaaccaag gtacgtgacg gttacgatgc
     3541 cgatagcgat gacgaccacc cggatggcat gggaggagat actctgtgcg tagcgtgccc
     3601 ctgcgtaacc gccgatggtg ctgccggcag ccatcaccag ccccgcatgc cagtttatga
     3661 aatggccgtg tgaaagtatg accatgcaga tagcggccga aacggtagcc gccacgttct
     3721 tcatggcatt tatctggtgg gcgtcgtgca gcttggtaaa acccaggaag gccagcatta
     3781 taaagccgac accggcgccg aaatatccgc cgtagatggc gaccgggaag agggcgatgc
     3841 cgatgatcac caggggtttt acgcgtttgc tgcggctctt aaggtgcttg tgcaggtagt
     3901 ggtgcagaaa gggctggaac gcgaacagca gtacggcgaa caatataagg ccaggcacgt
     3961 accgctcaaa cgtcatgctt ggcgtccggc gcagcagcag ggcaccgata accgcaccga
     4021 cggcccaagg cgctagcagc caggcgtatt tccagggagt cttgcgcagg tatttccagt
     4081 agccgacggc cgatgccagc tggccgggaa agatgaccac gttaccggtg ccgttggcga
     4141 tgagtgccgg caggccggtc gcgagtagca cgggaaaccc catgagcatc ccgccgccgg
     4201 caatggcgtt catgctgccg accgccaagc cggcgccaaa aagcagaata tctttcagat
     4261 ccatgtttgt ttttgagctt ctgagagtaa gtatagcagc ctagctattc tcgaaaaaca
     4321 ccgtgccggt ccgataggcg gcaagatagt gtttgcacga tgcgaatagt ggctcgggta
     4381 tggcatctag gggataccaa tcccaacttt ctattttgtc cggctccatc actttcggtt
     4441 cgccacttat ccattcggcg accaggccga catctatgta atgcttgggt gcataatcgc
     4501 ggatattact gaggcacagg aactgcggcg tgcttatacg aatgtcgtcg ccgatttctt
     4561 cctgcagttc ccgcatgatt gtttcgttga ccgtttccag atgttccatg tggccaccga
     4621 ctcccgcgta ttcgccgtcg ccgtggctgc ttttccgctt gcctaacaat atgtacttgc
     4681 cgtccttgat gagcaataag ccgatgccga ctttcggcct gtcactgctc atgccagctc
     4741 ctcgaccacg gcgttacggg gctcggtgcc ggctatgaac gggatgtcgg ggtgcatggc
     4801 cgcgcgcagg gcgcgccaga aagtgccatg ggcgacgacc agcaccgtgt cgtcaggcag
     4861 gtcatggagg tactggtagg cttggcggcc gcgctgcagg agcgattcga tcggctcgac
     4921 gccggcaata ccgtttatgt cctcctcgcc ctggctaccc caggctttgc cctgcagcga
     4981 gccgtaattg cgctccttga agaggtcatt tatatcgata ttactcaccg ggtagccgat
     5041 agtctgggca atgatgacgg cggtgtcgaa cgctcggcta agcggcgagg acacgatacg
     5101 gtcgatgtgc agctcccgtg ccgccttgcc ggcggtcttg gcttgctccc ggccgaggct
     5161 ggttaagggt gtgtcggatt ggccggcgaa tgtttcgtcg acattagcct ggctctggcc
     5221 atgacggaca aaatagagct ttttcaccgg cggctccgga tgcggttggc tatccactgc
     5281 cgcttgagca gcgtgagggt caagccggac aggaagaagc cgatgaccgg caccagagcg
     5341 ttgatccagg ggtcaatatg caccacgaac tgcaaatagg cgacgacgat ccagtaggtg
     5401 aggaacatga tggccaggcg ccgggtccag ctggtttccc agcccttgtc ggcctccacg
     5461 cggcggttgc gcgcttcgag ctgctcgatg cgcttgagca aggagttatt ggtaggcatg
     5521 cgtatattgt agcggaccgg gcaatagtta acttgctaag ctttgcaggt attactttag
     5581 ctctagcgta atgaccggca agcgctatac aatgaccctt gtaatgaagc tcgatatgcc
     5641 ccccggaaat tatgtcgttg ccgtcagcgg cggcgttgac agcgtggttt tattggacct
     5701 gctgcgttat cattccggac ttaaactgac ggtggcgcat ttcgaccacg gtatacggtc
     5761 ggattcccgg ctcgaccgca tcttcgtcca gcagctcgcc caccgctacc agctaccgtt
     5821 cgtcttcgat gccgtcacgc tcgggccagg cgccagcgaa gcagcggccc gcaaggccag
     5881 atacgcgttt cttaagcgcg cgcaacaggc cgccggggcg caggcgatca tcactgccca
     5941 tcaccaggac gatatgctcg aaacagcggt gctgaatctg ttgcgcggca ccggccggaa
     6001 ggggcttagc tcgttgacga cgggtgaggg ggttatccga ccgctgctcg aagtgccgaa
     6061 gagcgaactg ctcgactacg cccgccgcca ccgcttaagc tggcgcgaag acagcactaa
     6121 caacgacacc aactatctgc gcaaccacat ccgtcatgtc atgctgccct cgtggcgtga
     6181 ggccgacaaa cagcgcctcc tgggtatcat ccgacatatg cgcgaggtca acggcagcct
     6241 ggacgcggtg ctcgacgatt cgttgacccc gcagctgaag cgggaatggt tcgtgctgct
     6301 gccccataat gtggcgcggg aagtcatggc ctcctggctg cgaagcgccg atatccgcga
     6361 gttcgaccgc cggacactcg aacggttagt ggtcgccggt aaggttgctg aggcgggtaa
     6421 agcgattgat gtcgtcaagg ggcggcgcat gcgagtcggt cgcgaccacc tggcgcttga
     6481 agtccactga ggagtgactg aagttaagcc ataatattgg cactcaggct gtttgagcgc
     6541 tagatattca tttggcaggg gtatataatg gatattcgta tggataaatc accgctcaag
     6601 aacaacaaac agaagcccgg cgggagccgc cgcggcctca agaacgccgg gttcatcgcg
     6661 ctcatcattt tggctgcgct gataatcctg gctgctacca gccaaccgag caatctcaag
     6721 accatcccgg tgacgaccgc cgtgcagcag gctaacgccg gcgactattc caagatcacc
     6781 gtcaacggca accaactgac catcaccaag aagggtgata accaccccac actcaaatcg
     6841 tacttggagc cgaacgccac catgaaggac gaaggcttca attactccaa ggtagaaatc
     6901 gatccacagc cggagagcaa cagcagctcg gtctggctga ccctcgcctc gaccatcctg
     6961 ccggtcatcc ttatcggcgg cctgctgttc ttcatgctgc gtagcgccca aggacagggc
     7021 aaccaggcga tgagcttcgg taaaagccgc gcacggctgt atggcaatga aaaggacaag
     7081 gtcacgttcg ataacatcgc cgggagcggc gaggctaagc aggacctcga agaagtcgtt
     7141 gagtttttga agtatcccaa aaagtacgcc cacatgggcg cccgcatacc gaagggggta
     7201 ttgctggtcg gacctcctgg taccggcaag accatgctgg cccgggccgt cgccggcgag
     7261 gccaacgtgc cgttcttcag tatttccggt tcggaattcg tcgagatgtt cgtgggcgtc
     7321 ggtgcctccc gcgtccgcga tttgtttgcc aaggccaaga aaaacgcgcc ctgtattatc
     7381 tttgtcgacg aaatcgacgc cgtcgggcgc cgccgcggta gcggcatggg aggcgggcac
     7441 gacgagcgcg agcagacgct gaaccagatt ctggtggaga tggacggctt cgagcagggg
     7501 cagagtgtca ttgtattggc cgccactaac cgcgctgatg tgctcgatcc ggcattgttg
     7561 cgcccgggtc gtttcgaccg ccgcgtagac atcggtctgc cggaccgcaa ggaccgcgaa
     7621 gctatcctca aagtgcactt cgccaaaaaa ccgctcgcca aggccgtcga tctcgacgcg
     7681 ctggctgcca agagcgccgg ttcctctggg gcggacctgg caaacattgc caacgaatcg
     7741 gccatcattg ccgcccgtaa taaccaccag gaaatctcgg ccgacgatgt gaccgaagcc
     7801 ttcgagcgtg tggccatcgg tgctgaacgc aagagcaagg tcatgaccga aaaagataag
     7861 ctgacaactg cctaccacga aagtggccac gcgcttgtcg ggcacgtcct gccggacagc
     7921 gatccggtgc acaaggtgac cattattccg cgcggcggta ccggaggcgt gacctggttc
     7981 ctccccccgg aagaccgtgt ctacacttcg cttgttgaat tcaaagacgt tctggcccg
//
LOCUS       DASSFD010000196         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1359505, whole genome shotgun sequence.
ACCESSION   DASSFD010000196 DASSFD010000000
VERSION     DASSFD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359505"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFH39_04805"
     CDS             complement(<1..235)
                     /locus_tag="VFH39_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925124.1"
                     /translation="MSEATVPVRVERPAPHQDYPAALTIVALMFVGTLVSLLWFLHTP
                     SNYQERFNLSFALHGSLSVITGGSPDNPMPLYYW"
     gene            complement(228..1754)
                     /locus_tag="VFH39_04810"
     CDS             complement(228..1754)
                     /locus_tag="VFH39_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6925125.1"
                     /translation="MKSPFLESVQVKKMLAVNLALAAAYFVGLFVVFQRGNMALFITL
                     LVGEVFHLWQLATLTHAAWDTRTSHPFDPALAPAVDIYITVAGEPLSVVEKTVQAATR
                     IDYPNYKVYILNDGLVAKKDNWQDMETLAKKYQNLGVHCITRTTPGGAKAGNINHALS
                     KTRAPFVAILDCDHIPNREFLRRMMGYFVEDDVAFVQSPQYYRNHTKTFVANAAWGQQ
                     TLFFGPICRGKDRTNSLFMCGTNMVIRRKALDSVGGMKQDSITEDLLTSIVMHAKGWR
                     SVYVPRILARGLAPEDLSSYWQQQFRWARGSLEVLTKYNPLFLRGLSFAQRLQYLASV
                     TYYLTGLIVLVDAALPLFYFYAGLIPVTAATMSIALLFVPYIFLTLYLLQRISNYSFS
                     FRAIAFSLASWPIYALALLLTVFRVRSGFKVTAKEGVSGNYFGLAAIHLLYTLATLIG
                     IGFAFHRDGLSTSLITNGSWGLLYVVMFAPFIRAAMSDRVASILPYDELELPAEVIHE
                     "
     gene            complement(1751..>2412)
                     /locus_tag="VFH39_04815"
     CDS             complement(1751..>2412)
                     /locus_tag="VFH39_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET6925126.1"
                     /translation="LTADNLEKHSSDHLAVLNPSYFSPAAYRIFARVDGTHGWNGLIG
                     SSYTILHEAGTAPLGSSHSAGVPPDWVALNTQTGVLTAAAGHDTDFGYDAFRAVWRTA
                     LNYRWSGDSRSRKAMDDWKLLEQQWGQHGRLAARYHHDGSAAVSYESNALYGGVLPYF
                     QTVSSGDAASIINDKLAPLFADHGRALRQPLNYYDNNWVWFGLALYAGQSPDLAQGVS
                     S"
BASE COUNT          520 a          668 c          790 g          434 t
ORIGIN      
        1 gccagtagta cagcggcatg gggttatcgg gcgagccgcc ggtaatgacg ctcagggagc
       61 cgtgcagcgc gaacgacagg ttgaaccgct cctggtagtt gctgggggta tgcaggaacc
      121 acagcagcga caccagcgta cccacgaaca tcagggctac aatggtcaat gctgcggggt
      181 agtcctggtg cggagccggc cgctcgacgc ggaccggtac ggtagcctca ctcatggata
      241 acctccgccg gcaactccag ctcgtcgtac gggagtatcg aggctacccg gtcggacatg
      301 gccgcccgga tgaacggagc gaacatcacc acgtacagca agccccaact gccgttggta
      361 atgagcgagg tggacagccc gtcgcggtgg aaggcgaagc cgatgccgat cagcgtggcg
      421 agcgtgtaga ggaggtggat agccgccagt ccgaagtaat tgccgctgac gccttctttg
      481 gcggtcacct tgaaaccgct gcggacgcgg aagactgtca gcagcagtgc cagggcataa
      541 atcggccacg aggcgagcga aaaggcgata gcccggaacg agaatgagta attgctgatg
      601 cgttgcagca gatacagggt taggaaaatg tacggcacga acaacagggc gatggacatg
      661 gtggcggcgg tgacgggaat gagtccggca tagaagtaga acagcggcag cgcagcgtcg
      721 accaacacga tcaaaccggt cagatagtag gtgacggatg ccagatactg caggcgctgg
      781 gcaaaactca agccacgcaa aaagagcggg ttgtatttgg tgagaacctc cagactaccg
      841 cgggcccagc ggaactgctg ctgccagtag gagctcaggt cttccggagc cagtccacgg
      901 gccaatatgc gcggtacgta cactgagcgc cagcctttgg cgtgcatgac gatggaagtc
      961 agcaggtcct cggtgatact gtcctgcttc atgccgccga cgctgtcgag cgctttgcgg
     1021 cggatgacca tgttggtgcc gcacatgaac agcgaattgg tgcggtcctt gccgcggcag
     1081 atcgggccga aaaacagtgt ctgctgaccc caggccgcgt tggcgacgaa cgtcttagtg
     1141 tggttgcggt agtactgcgg cgactgcacg aaagcgacgt catcctcgac gaagtagccc
     1201 atcatacgcc ggaggaattc gcggtttggg atgtggtcgc agtccaggat ggcgacgaac
     1261 ggagcccgcg tcttactgag cgcatggttg atattgccgg ccttggcgcc accgggcgtg
     1321 gtgcgggtaa tgcagtgcac accaaggttt tgatacttct tggccagcgt ctccatatcc
     1381 tgccagttgt cttttttggc caccagcccg tcattcaaga tatatacctt gtagttcggg
     1441 taatcgatgc gcgtagcggc ctggacagtc ttttcgacca ccgacagcgg ttcgccggca
     1501 acggttatat agatgtcgac cgccggcgct aaagccgggt cgaacggatg agaggtgcgg
     1561 gtatcccagg cggcatgcgt cagggtagcc agctgccaca gatggaatac ttcacccacc
     1621 agcagcgtga tgaacagcgc catattgccg cgctggaaca ccacgaacaa accgacgaag
     1681 taagcggcgg ccagggctag gttgacggcc agcattttct ttacctggac cgattcgaga
     1741 aacggggact tcatgagctg actccttgcg ccaggtcggg tgattgaccg gcgtacagcg
     1801 ccaagccgaa ccacacccag ttgttgtcgt aatagttgag cggctggcgc agggcacggc
     1861 catggtcggc aaacaacggt gccagtttgt cgttgataat agacgctgca tcaccggacg
     1921 acacggtctg gaagtagggc aggacaccgc cgtacaaggc gtttgattcg tagctgacag
     1981 ctgctgagcc atcgtggtgg tagcgggcgg ccagccggcc gtgctgcccc cactgttgct
     2041 ccagaagctt ccagtcatcc atggctttac gggaccggga atcaccggac caccggtagt
     2101 tcagggcggt gcgccatacg gcacggaagg cgtcgtagcc gaagtcggta tcatgaccgg
     2161 ccgcggccgt gagcacaccg gtctgcgtat tgagcgctac ccagtcgggc ggcaccccgg
     2221 cgctgtgcga cgaaccgagc ggggcagtgc ccgcctcgtg cagaatggta tacgacgagc
     2281 cgatcagccc gttccagcca tgtgtgccgt cgacccgtgc gaatatccgg taggctgcgg
     2341 gcgaaaagta ggaagggttc aggacagcca agtgatcgct actgtgcttc tcgagattgt
     2401 cggccgtcag gt
//
LOCUS       DASSFD010000197         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1258781, whole genome shotgun sequence.
ACCESSION   DASSFD010000197 DASSFD010000000
VERSION     DASSFD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258781"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFH39_04820"
     CDS             <1..723
                     /locus_tag="VFH39_04820"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET6925127.1"
                     /translation="MSAWFVLARLTDRTDVVDSAWGLGFVYVAWLSYLMNPRSGWKLL
                     AAVFVSVWGMRLFVHLTSRSLKRSEDYRYVEFRKKWGGAYWGKAFVRIFLLQGLLLFL
                     VSLATIAIMMTVSWAMTWLAVAGFVTWAIGIVYEAVGDWQLRQFMKSRKPGQIMAHGL
                     WRYSRHPNYFGEVIAWWGAGLVALSFGQWWGLLGSLTITVLITKVSGIPPLEKHYAGN
                     AAYEQYKARTSVLVPLPPRKTS"
     gene            799..2235
                     /gene="pyk"
                     /locus_tag="VFH39_04825"
     CDS             799..2235
                     /gene="pyk"
                     /locus_tag="VFH39_04825"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET6925128.1"
                     /translation="MTDITHSVPNVPLASFKRTKIIATVGPATDNYEAIYGLIKAGAN
                     GLRLNFSHETHEAADRHIPWIRKASAAYGKPVAIIQDLQGPKIRLGDFDGVFNVQKGQ
                     SLTFQYQADFAASGHLPTQYDLSTKVKRGERIFLYDGRIQTVVTSVHGSVVYARAEND
                     GILIKRKGMNLPDTDFGGDILSAKDRADLAYGSTQDIDYVALSFVQTADDIVNLRRLL
                     NDLGSTAKIIAKIETNAAVENIEAIVQEADAVMIARGDLAVETPAESVPIVQRQIIGL
                     GIRYAKPTIVATQMLISMTEMPEPTRAEVSDVATAVLVGADCVMLSDETANGQYPIEA
                     VQVMKRVIRYTEAHAPLKAVFRENDDHSRQTAISKAVLSLAESVQAKAIVAETRSGAT
                     ALQIAARRCDIPLIAVTSENRTAQQLAITYDIKSYVRPVDAQAAIKLTDWLHKHGVLC
                     KGDMVVTASGRHPGVVGTTDTIKVRVIE"
     gene            2341..>2806
                     /gene="pgk"
                     /locus_tag="VFH39_04830"
     CDS             2341..>2806
                     /gene="pgk"
                     /locus_tag="VFH39_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6925129.1"
                     /translation="MSFNKKTINDIDLHGKTVLLRADYNVPLEHGKITDDYRIQQSLP
                     TIRALLDKNVKLIICSHLGRPDGKPNPDLSLFPVAKRLQELLERDVEFATDCVGERAV
                     KAAESLQPGQVLLLENLRFHPEEEANDPGFAKALASLAEVFVQDGFGVVHRAH"
BASE COUNT          606 a          819 c          863 g          518 t
ORIGIN      
        1 atgtcggcct ggttcgtact ggcccgtttg acggatcgta ccgatgtcgt cgacagcgcc
       61 tggggactcg gcttcgtgta cgtcgcgtgg cttagctacc tcatgaatcc gcgcagcggc
      121 tggaaactgc tggccgccgt atttgtcagc gtatggggga tgcgcctgtt cgtgcacctc
      181 accagccgga gcctcaaacg cagcgaagat taccgttatg tcgagttccg caagaagtgg
      241 ggcggcgcct attgggggaa ggcattcgtg cgtatttttc tgctgcaggg gttgctactg
      301 ttcctggtaa gtctggcgac catcgccatc atgatgaccg tatcatgggc catgacctgg
      361 ttagccgtcg ccggcttcgt aacctgggcg atcggtatcg tgtatgaggc cgtcggtgat
      421 tggcagctac gccagttcat gaaatcccgt aagccggggc agatcatggc ccatgggctg
      481 tggcgataca gtcggcaccc gaattacttc ggcgaggtta tcgcctggtg gggtgccggc
      541 ttggtggcct tgagcttcgg ccagtggtgg gggctgcttg gctcactgac cattacggtc
      601 cttatcacta aggtttccgg tatcccgccc ctcgagaagc actatgcggg caacgcggca
      661 tacgagcagt ataaagcgcg tacgtccgtg ttggtgccgt tgccacctcg caaaacctcg
      721 tagcctggcg agccgtctgg cgctgcgccc gttggcgtgc taccataagc gtaaacgaac
      781 ctattcggac gggtggaaat gacagatata acacacagtg tgccaaacgt gccgcttgct
      841 agcttcaagc ggacaaaaat catcgcgacc gtcgggccgg ccaccgacaa ctacgaggct
      901 atctacgggc ttatcaaggc cggcgctaac ggcttacggc tgaacttcag ccacgagacg
      961 cacgaagctg ccgaccggca cattccgtgg atccgcaaag cctcggcggc gtatggtaag
     1021 ccggtggcga ttatccagga cctgcaggga ccgaaaatca ggctcggcga cttcgacggc
     1081 gtattcaacg ttcagaaagg ccaaagcctg acgttccagt accaggcgga tttcgcggcc
     1141 agcggtcacc tgccgacgca gtacgacttg agcaccaagg tgaaacgcgg cgaacggatc
     1201 tttctgtatg acgggcgcat ccagacggtg gtgaccagcg tgcacggcag cgtggtgtac
     1261 gcccgggccg aaaacgatgg catccttata aagcgcaagg gtatgaacct gccggatacc
     1321 gatttcggcg gtgatattct gagcgcgaaa gaccgggccg atctggcgta cggttcaacc
     1381 caggatatcg attacgtagc cctcagcttc gtgcagaccg ccgatgatat cgtcaacctg
     1441 cggcgcctgt tgaacgactt gggtagcacg gcgaagatca tcgccaagat cgagaccaac
     1501 gcagccgtgg agaacataga agccatcgtt caggaagcag acgccgtcat gatcgcccgc
     1561 ggcgatctgg cggtggagac accggccgag agcgtgccga tcgtgcagcg gcagatcatc
     1621 gggctcggca tccgttacgc caagccgacc atcgtggcaa cacagatgct tatcagcatg
     1681 accgaaatgc cggaaccgac ccgggccgag gtttcggatg tggcaacggc cgtgctggtc
     1741 ggtgccgact gcgtcatgct cagcgacgag acggccaacg gccaatatcc gatagaagcg
     1801 gtgcaggtca tgaaacgcgt catccgctac accgaagccc acgcgccgct gaaggcggtt
     1861 ttccgggaga atgatgacca tagtcgccag acggccatca gtaaggcggt cctcagcctg
     1921 gccgagagcg tacaggccaa ggctatcgtc gccgagaccc gcagcggcgc cacagcgctg
     1981 cagatcgcgg cccggcgctg cgacatcccg ctcatcgccg ttaccagcga gaaccggact
     2041 gcccagcagt tggccatcac ctacgacatc aaaagctacg tgcggccggt cgatgcgcag
     2101 gcagccatca agctgacgga ttggctgcac aagcacggcg tactgtgcaa aggtgacatg
     2161 gtcgtgaccg cctccggccg ccacccgggc gtggtaggta cgaccgacac tatcaaggta
     2221 cgggtcatcg agtaagtttc cgcgatagcg cccggtaggt tatagtggga gtaagcgcta
     2281 ggattatgca gcacaaaccg ctacactatc aacaataagc cggatcttac gggtggggca
     2341 gtgagtttca acaagaaaac catcaacgac atcgatttgc acggtaagac cgtgcttttg
     2401 cgtgccgact acaacgtacc gcttgagcat ggcaaaatca ccgacgatta ccgtattcaa
     2461 cagagcctgc cgaccatccg cgcactactc gataagaacg taaaactgat aatctgttct
     2521 cacctcggcc gtccggacgg caagccgaac cccgacctca gcttattccc ggtggctaag
     2581 cggctgcagg agctgctgga gcgcgacgtc gaattcgcga ccgattgtgt gggcgagcgg
     2641 gccgtgaaag cggccgaaag cctgcagccg ggccaggtac tgctgctgga aaacctgcgc
     2701 ttccaccccg aggaagaagc caatgacccc gggttcgcca aagcgctagc cagcctggcc
     2761 gaagtgttcg tgcaggacgg gttcggagtg gtgcaccggg cgcacg
//
LOCUS       DASSFD010000198         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1485736, whole genome shotgun sequence.
ACCESSION   DASSFD010000198 DASSFD010000000
VERSION     DASSFD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485736"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..612
                     /gene="raiA"
                     /locus_tag="VFH39_04835"
     CDS             250..612
                     /gene="raiA"
                     /locus_tag="VFH39_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6925130.1"
                     /translation="MLERFEVHGVHAPVDDNLKRYAAKKIGNLDKYLSRHYKDSAHCE
                     VILKESKAKDKKHCTCEVNLHLPKETINIKESTINMYASIDVVEAKLKLLLKKYKDTR
                     VSGKLRRRLTARLSRQSA"
     gene            794..>2842
                     /gene="secA"
                     /locus_tag="VFH39_04840"
     CDS             794..>2842
                     /gene="secA"
                     /locus_tag="VFH39_04840"
                     /inference="COORDINATES: protein motif:HMM:NF006630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET6925131.1"
                     /translation="MNIVFKKILGDPQAKTVKRLRKRAQEVNALAEKYSAMSDRELRQ
                     QTDVLKKRLQKESLDKILPDAFAVVREAATRTLGQRHFDVQLMGGIVLHEGNVSEMRT
                     GEGKTLVATLPVYLNALTGKGVHVVTVNDYLAQRDAGWMAQVYHFLGLSVGAIIPDHS
                     FIYDPDFINEDHEDERMRHLRPATRQEAYAADITYGTNNEYGFDYLRDNMVREEDQLR
                     QRELHYAIVDEVDSILIDEARTPLIISAPSATSGTAYAQFARVVLQLKPEKDYETDEK
                     RKTVILTDSGVEKMEKALGIPNLYASENIRTIYHLQQALRAQALFKRDKDYVVTKDGE
                     VVIVDEFTGRLLAGRRYNEGLHQAIEAKEGVEVQEESMTLATISFQNYFRLYEKLAGM
                     TGTASTESEEFHQIYKLDVVEVPPNRPNARSDRTDRIYKTERAKFKAIVREIKALHTK
                     GQPVLIGTASIEKNEALGSLLGRAGVPHQVLNAKNNEREAKIVAQAGEKGAVTLATNI
                     AGRGTDIVLGEGVKELGGLFVLGSERHESRRIDNQLRGRAGRQGDPGVTQFFVSTEDD
                     LMRIFGGDRIANIMERLSVDEDTPIENRIISRSLEGAQKKVEGYHFDQRKNVVQYDDV
                     MNRHRKATYAMRKEILKAPDISKRIKLFIEDEVRTLAASPLLLSDELEDVLREVFPFD
                     E"
BASE COUNT          735 a          795 c          831 g          481 t
ORIGIN      
        1 gtccgccgta acgacggtgc cgtggaactt atacatacta acgatgctac agcgttcgct
       61 ctcagccgga cacctatcgc catcgtagaa aactaccaaa cagctgatgg ccatgtgaag
      121 attccggcag ccttgcaacc ctacatgggc ggcaaagtag agatttgagt cataggcacc
      181 tggcgcccgg ggcgtacaat ataagtgtga gctgcgcgca ctttgaaact aacggaaaag
      241 gagcaaggta tgcttgaacg gtttgaggta cacggcgtgc acgcgccggt agatgataac
      301 ttaaagaggt acgctgccaa aaagatcggc aacctggaca aatacctcag ccgccattac
      361 aaggatagcg cccactgcga ggtcattctc aaagagtcaa aggccaagga caagaagcat
      421 tgcacctgcg aagttaacct gcacttgccc aaagagacca tcaacatcaa agaaagcacc
      481 ataaacatgt atgcttccat agacgtcgtg gaggccaagc taaagctgct gctcaagaaa
      541 tacaaagaca cccgcgtgag cggcaagctc cgccgcaggc taacggcccg gctatcccgc
      601 cagagtgcct aacaagccaa ctccggaaag aggtctgatt acgcgcctga acctcgaaaa
      661 aggatatctc ttgaagcggg atggcggcgt ctgcggcaag aaggataaac aggctcgatt
      721 ttcttcggat atacgagtac aatgtttaga agttaattac caaaactaca accgggagag
      781 actgtaagct ggtatgaata ttgtcttcaa aaaaatctta ggggacccgc aggccaagac
      841 cgtcaaacgc ctgcgcaagc gcgcccagga ggtgaacgcg ctggccgaaa agtattcggc
      901 catgagcgac cgggagctgc gccaacagac cgatgtgctt aagaagcggc tgcagaagga
      961 aagtctggac aagatactgc cggatgcctt cgcggtggtg cgtgaggcgg cgacgcggac
     1021 gctcgggcag cggcacttcg acgtccagct catgggcggc atagtcctgc acgaaggcaa
     1081 tgtctcggaa atgcgtaccg gcgaaggcaa gacgctggtg gcgacactgc cggtgtacct
     1141 gaacgcactc accggcaaag gcgtgcatgt ggtaacggtc aacgactatc tggcacagcg
     1201 cgacgccggc tggatggcgc aggtgtatca ctttttgggc ttgagtgtcg gcgccatcat
     1261 ccctgaccac tccttcattt atgacccgga tttcataaac gaagaccacg aagacgagcg
     1321 tatgcggcac ctgaggccgg caacccgcca ggaggcctac gccgccgaca tcacctatgg
     1381 caccaacaac gaatacggat tcgactacct gcgcgacaac atggtgcgcg aagaggatca
     1441 gctgcgccag cgcgagctgc actacgccat cgtcgacgag gtggactcca tacttatcga
     1501 cgaagcccgc acgccgctga tcatcagcgc gcccagcgcc acgagcggta cggcctacgc
     1561 ccagttcgcc cgggtggtgc tgcagcttaa gcccgaaaag gactacgaaa ccgacgaaaa
     1621 gcgcaagaca gtcatcctga ccgacagcgg agtggaaaag atggaaaagg ccctgggtat
     1681 tccgaacctg tacgcctccg aaaatatccg caccatctac cacctgcagc aggcactgcg
     1741 ggctcaggcg ctctttaagc gcgacaagga ttacgtcgtt accaaggacg gcgaagtggt
     1801 catcgtcgac gagttcaccg ggcggctgct ggctggccgg cgctacaacg aaggcctgca
     1861 tcaggccatt gaggccaagg aaggtgttga ggtgcaggaa gaatccatga cgctcgctac
     1921 catctccttc cagaactact tccgtctgta cgaaaagctg gccggcatga cggggacggc
     1981 ttccacggag agtgaagagt tccatcagat ttacaaactg gatgttgtgg aggtgccgcc
     2041 gaaccggccg aacgctcgct ccgaccgtac ggaccgtata tataagacgg agcgcgccaa
     2101 gttcaaagcc atcgtacgag agataaaggc attgcacacc aagggccagc cggtgcttat
     2161 aggtaccgcc agcattgaaa agaacgaagc ccttggcagc ctgctgggcc gtgccggcgt
     2221 gccgcaccag gttctgaatg ccaagaacaa tgaacgcgaa gccaaaattg tcgcgcaggc
     2281 aggcgaaaaa ggtgccgtga ccctggccac caacattgcc ggccgcggta ccgacattgt
     2341 gctgggcgag ggcgtcaagg aactgggagg cttgttcgtg cttggcagcg agcgccatga
     2401 atcccgccgt atcgacaacc agctgcgcgg ccgcgccggg cggcagggcg acccgggcgt
     2461 gacgcagttc ttcgtaagta ccgaagacga tctgatgcgt atattcggcg gcgaccgcat
     2521 tgcgaacatc atggagcgcc tgagtgtcga cgaagacaca cccatcgaaa accgtatcat
     2581 cagccgctcg ctggaagggg cgcagaagaa ggtggaaggc taccacttcg accagcgcaa
     2641 gaacgtcgtg cagtacgacg atgtcatgaa ccgccaccgc aaggcaacct atgccatgcg
     2701 taaggagatt ctgaaggcgc cggacataag caaacgcatc aaacttttca tcgaggacga
     2761 agtgcgtacg ctggcggcat cgccgctgct gctgtcggat gagctggagg acgtgctgcg
     2821 tgaggtcttc ccgttcgacg aa
//
LOCUS       DASSFD010000199         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_982571, whole genome shotgun sequence.
ACCESSION   DASSFD010000199 DASSFD010000000
VERSION     DASSFD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982571"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..881
                     /locus_tag="VFH39_04845"
     CDS             261..881
                     /locus_tag="VFH39_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925132.1"
                     /translation="MSIWWLVVILGFIGASGIGFIWLVVHLGGAGGSKKGLPTSVEEA
                     AEKDVEHIFNDEFREELRNRGRLHFEKIIGENAMFLQQDLRLTTSQLNEYMKSEITKK
                     LQEEFEKYEQSIMDAKQLAIESIEKTNAAIDEQRKLLGDQVQKEIVAEKQQLVARFEQ
                     NMTDIVNHYVMAAVGNQIDLNDQLEYILADLEVNKQALIQDIANGA"
     gene            871..1575
                     /locus_tag="VFH39_04850"
     CDS             871..1575
                     /locus_tag="VFH39_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925133.1"
                     /translation="MEPDHKHLTGLPLSLVSPVDLGRVIRELEQLENHLNELRIRTPG
                     QEIKMPKTTKLMDDLLNLNQLNLLQAADRKTLKQFLSVVKQRAPRLHISFSADPSPLF
                     VEKIMTWLRKEVHPVVLLTTGLQPNIGAGCIVRTTNKYFDFSLAKDFEKKRDVLMQAL
                     RDAPVKEAAPQAVTTTPAHAQQTVSTPAATAPAAPEKPLAKPTNTYLATPASPASATT
                     EPGAPKLAAAHAEAHR"
     gene            1572..3200
                     /locus_tag="VFH39_04855"
     CDS             1572..3200
                     /locus_tag="VFH39_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HET6925134.1"
                     /translation="MTSGNQHFDRLVEAGNPVGEVIAVEKFLIRCSGLQPCAVNALIM
                     FEDGSKGFVSQVQDGYVVILHLGSEALRVGMVAVVQHQELVCKVGKDYIGRVITATGE
                     PLDGKGPIAASATWPVFNPAPNLYERKLVEDQLESGVTAIDALFPMVRGQRMALLGDS
                     KSGKSTVVTQLAINQKNTDQIVVYCLIAKRRSDVDTLLSRLTENGGLEKAIVVVSTMF
                     ESLIMSYIAPYVACAMAEYLWQACDQDTIIVYDDLTSHAHAYREVALLGGTSPGRDSY
                     PGDMFYAHSSLLERAGRLDRNGKCLTSIPVVHAANGDITAYLPTNIMSITDGQWILDM
                     DIFRSGVRPALNIGLSVTRAGGVGHNKRQKALAAQTLKILADYRQAEEFSHFGSELGD
                     DAKHALATGKLIFEVLTQSPSDTYSLMAQQLMMDIVLNLEEGAEVDVNTLKLNVGEFA
                     AKVTGDDDYDKFRDELKKKCVTLPPKPEAEGEAAEGDAGAAADAAKPDEKKPAADDKK
                     ADEKSDKHDEHGKADEKHDDKAKESEKELAEAKK"
     gene            3319..>3886
                     /locus_tag="VFH39_04860"
     CDS             3319..>3886
                     /locus_tag="VFH39_04860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6925135.1"
                     /translation="MRRPNEIARDEASMATLVELTSVFEGIASMRIAQTKNQVLESTK
                     FFNALWKIYSQLRVDALFSFGREADEKPLIDKELYIIITAEGGFSGDIDQKLMQLMLK
                     TFNKDRNEIIVIGHHGAVQLAQRGISYKKYYKLPSKDQNINVTPIIRDVQQYKSATVF
                     YQEYVSLMNQDVKSITLSNAVKSRGDEAD"
BASE COUNT          927 a         1136 c         1160 g          663 t
ORIGIN      
        1 cttatttcca tcggtcgtaa tccgatggct aagaagcaca tcgtggccgg tatcgtgcgg
       61 gtggtgctga tggggataat tgttttctta atcggtctgt ttggcgtata cttgctatta
      121 aagctctagc gttatagcat caacgttgct ataccagaga gagataacgg ctgcgacatg
      181 aaaacggtgg aaaaaacaga caacatacag cagaccattt ataccgcgcg gtgctcccgg
      241 gcggctttgg agcgtagggc atgagcatct ggtggctggt ggtaatcttg ggcttcatcg
      301 gcgcgagcgg catcggcttc atctggcttg tggtgcacct tggtggtgct ggcggcagca
      361 agaaggggct ccccacttcc gtcgaggaag cggccgagaa agatgtcgag catatcttca
      421 acgacgagtt ccgcgaggaa ctgcgtaacc gcggccggct gcacttcgaa aagatcatcg
      481 gtgagaacgc catgttcctt cagcaggacc tgcggttgac gacttcgcag ctcaatgagt
      541 acatgaagtc ggaaatcacc aagaagctgc aagaagagtt cgagaaatat gaacagtcga
      601 ttatggacgc caagcagctg gccatcgagt cgatagagaa gaccaacgcc gccatcgacg
      661 agcagcgcaa actgctgggc gaccaggtcc agaaggaaat cgtcgccgag aagcagcagc
      721 tggtggcccg gttcgagcag aacatgaccg acatcgtcaa ccactacgtc atggcggccg
      781 tcggcaacca gatagacctc aacgatcagc tggaatacat cctggccgac ctggaggtga
      841 acaagcaggc gcttatacag gacatagcca atggagcctg accacaaaca ccttaccggc
      901 ttgccgctca gcctggtcag tccggtcgat ctgggccggg tgatacgcga actggagcag
      961 ctggagaatc atctgaacga gctgcgtatc cggacgccgg gccaggaaat taagatgccc
     1021 aagaccacca aactgatgga cgacctgctc aacctgaatc agctgaacct gctgcaggcg
     1081 gccgatcgca agaccctcaa acagttcctg agcgtcgtga agcagcgggc cccgcgcctg
     1141 catatcagct tcagtgctga cccgtcgccg ctgttcgtag agaaaatcat gacctggctg
     1201 cgtaaagaag tgcacccggt tgtcctgctg accaccggcc tgcagccgaa catcggtgcc
     1261 ggctgcatcg tgcgcaccac caataagtat ttcgatttct cgctggccaa agactttgag
     1321 aagaagcgcg acgtgctgat gcaggcgtta cgggatgcac cggtgaagga agcggcgccg
     1381 caggccgtca cgaccacgcc agcccatgcc cagcagaccg ttagcacacc ggccgcgacg
     1441 gccccggctg ctccggaaaa accgcttgcc aagcctacta acacctacct ggccaccccg
     1501 gcatcgccgg catcggcgac gaccgaaccg ggagcgccaa aattggctgc cgcccatgcg
     1561 gaggcgcacc gatgacgtcc ggcaatcagc acttcgatcg gctggtcgag gccggcaatc
     1621 ccgtcggcga ggtcatcgcc gtcgagaaat tccttatccg ctgcagcggc ctgcagccgt
     1681 gcgccgtcaa tgcgctgatc atgttcgagg acggcagcaa gggattcgtc agccaggtcc
     1741 aggacggcta tgtggttatt ttgcacttgg gctctgaggc gctgcgcgtc ggcatggtcg
     1801 ccgtggtgca gcaccaggag ctggtctgca aggtgggcaa ggattacatc ggccgcgtca
     1861 tcaccgcgac gggcgaaccg ctggacggca aggggccgat cgctgccagc gctacctggc
     1921 cggtgttcaa tccggcaccg aacctgtacg aacgcaaact ggtcgaagac cagctggaat
     1981 ccggcgtaac ggccatcgac gcgctgttcc cgatggtgcg cggccagcgt atggccctgc
     2041 tgggcgacag caagtccggc aagagcacgg tggtgacgca gctggccatc aaccagaaga
     2101 acaccgatca gatcgtggtc tactgcttga tcgccaagcg ccgaagcgat gtcgacacgc
     2161 tgctcagccg tctgacagag aacggtggtc tcgaaaaggc catagtcgtg gtgagtacca
     2221 tgttcgagtc gctcattatg agctacatcg cgccgtacgt ggcctgcgcc atggccgagt
     2281 atctgtggca ggcttgcgac caggacacca tcatcgtcta cgacgacctg acgagtcacg
     2341 cccatgcata ccgggaggtc gccctgctcg ggggcaccag ccctggccgt gacagctacc
     2401 ccggcgacat gttctatgcc cactccagtt tgctggagcg ggccggccgt ctggaccgta
     2461 atggcaaatg tcttaccagt atcccggtgg tgcacgcggc gaacggcgac atcaccgcct
     2521 acctgccgac caacatcatg tccatcaccg acggacagtg gatcctcgac atggacatct
     2581 tccgctccgg tgtccgtccg gcactcaaca tcggactttc cgtcacccgt gccggtggcg
     2641 tcggccataa caagcgacag aaagccctgg ccgcccagac actcaagata ctggccgact
     2701 accgtcaggc ggaggaattc tcgcacttcg gttctgaact gggtgacgac gcgaagcatg
     2761 cgctggccac cgggaagctc attttcgagg tgctgaccca gagcccgagc gacacctaca
     2821 gcctgatggc tcagcagctg atgatggaca tcgtgcttaa tcttgaggag ggtgcagagg
     2881 tagatgtgaa taccctgaag ctgaacgtcg gcgaattcgc cgccaaggtc accggcgatg
     2941 acgattacga caaattccgg gacgagctca agaagaagtg cgtgacatta ccaccgaagc
     3001 cggaagccga gggcgaggcc gccgaaggcg acgccggggc ggcggccgat gcggccaagc
     3061 cggacgaaaa gaaaccggca gccgacgaca agaaggccga tgaaaagtcc gacaaacacg
     3121 atgagcacgg caaggccgac gaaaagcacg acgacaaagc caaagagtct gaaaaagaat
     3181 tggcggaggc caagaaatga gcttaagtac tgcagaatgg ttcaggggag agcagccgga
     3241 ttgccggatt cattccggca tgccgcgcgt tgagaggggg tgcccccctc tcaataccct
     3301 ggcggaggcc aaaaagtaat gcgccggccg aacgaaatag ccagggatga agccagcatg
     3361 gccacgctcg tggagctgac cagcgtgttc gaaggtatcg ccagtatgcg catcgcccag
     3421 acgaagaacc aggtgctgga aagtaccaag ttcttcaatg ccttgtggaa gatctacagc
     3481 cagctgcggg tggacgcgct cttcagcttc ggccgcgaag ccgacgagaa gccgctcatc
     3541 gacaaagagt tgtacatcat catcaccgcc gaaggcggtt tctccgggga catcgaccag
     3601 aagctcatgc agctcatgct caagacgttc aacaaagacc gtaacgagat tatcgtcatc
     3661 ggccaccacg gcgccgtcca gctggcgcag cgcggcatca gctacaagaa atactacaaa
     3721 ctgcccagta aggaccagaa catcaatgtc acgcctatca tccgggacgt gcagcagtac
     3781 aaaagcgcga cggtgtttta ccaggagtac gtctcgctca tgaaccagga cgtcaagagt
     3841 atcacgctca gcaacgccgt caaaagccgg ggcgacgaag ccgaca
//
LOCUS       DASSFD010000200         6690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_422790, whole genome shotgun sequence.
ACCESSION   DASSFD010000200 DASSFD010000000
VERSION     DASSFD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422790"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFH39_04865"
     CDS             complement(<1..285)
                     /locus_tag="VFH39_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925136.1"
                     /translation="MPSLRLQRLPHLSTLLSFVLIFGLIGTAWLLHSHASTPAVAVQP
                     ESGSVASPATVVNDPTAGGGKAVRFEKAVIASHGTACGWTTKAPAYKHVIW"
     gene            498..3080
                     /locus_tag="VFH39_04870"
     CDS             498..3080
                     /locus_tag="VFH39_04870"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HET6925137.1"
                     /translation="MASILSRPPKQPEHDDSGVVLLLQVPRTNDKKELAAEQMFASLH
                     GLLTLPSNKFMQPTMRERLSFEIAVIGKRIGFYVWVPNYLKSFVEEQIYAQYPNVQIS
                     EVPDYTMTPDGGFTTRLVTEMKLTAADPLPIKTFQSFEVDPLAAITATLAKFEESEEA
                     WIQLVIRPATSNWHKKSERYIAGLRAGKSNSMSGVLGALWAPPEYKSEGAKLTDYEQT
                     RAGGAEEKSQKLAFETSVRIVYRGNVPAQQARLHMQSIVASYKQFNTTYLNGFAAKRV
                     MDDPYLLALYRDRRFGKSGAILNIEEVATMYHLPHTTVETPYILWAASQTAEPPANLP
                     VVTSTYNPNISPIAATNFRGHNTMFGLPRSDRGRHLYIIGQTGVGKSGMLELLTISDI
                     YSPFGFAVIDPHGDYALNILRRIPAERAADVIYFNPADTDYPIAFNPMEVNDPKLKTH
                     TVSELIGVLKRMFESWGPRLEYILRYSLLALLDYPDATMLDITRILTDKRFRMDVLKY
                     VEDPVVKQFWTVEFASWNEKFATEAVAPVLNKVGAFTANPLVRNIIGQPKSSFNIRHI
                     MDQRKILIVNLSRGLVGEDNAALLGALLVTKIQLAAMSRADIPAEERSPFYLYVDEFQ
                     NFATDSFAVILSEARKYGLNLTVANQYIAQMSMEVKDAVFGNVGSIIAFRMGADDARG
                     MLRYFEPKFEEYDLVHMHNRHFVISLTIEGEKVPAFSAISLNLPPQQEDHSAVIVERS
                     RAQYAVSRQYVERYVQERYLTDSTPEAKKMPAANPITAPAAKPNVSRAAGASDQKAAV
                     LPQLMKAIHRDVAPGDQPASEPPAKPKRKRTRKRKKKTAAAPPSDAGASTQELQGSGE
                     IRLK"
     gene            3162..4781
                     /locus_tag="VFH39_04875"
     CDS             3162..4781
                     /locus_tag="VFH39_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET6925138.1"
                     /translation="MNPTAPATTRKSRRVPLANVAVLLIATSMFGQVLGFLRTKLINA
                     NFNTPHTPVGQNAGVYFAAFSIPDFFFFTIAAGALGVAVMPYLSDRLHKGDRKGVWEL
                     SASLLNFLAIIMAGVALVIIVFAKPLIHHVVAPGLTPEQLNNAAMLMRLLALNPLLFT
                     VSGILTSAQQTMGRFFFYAIAPLFYNLCIIISIYAFRNNIGIVGLGIGALIGAILQLV
                     VVAFGSMRLSFHWHPRIMWRSGEFRSMLKQLPPRSLDQGMDQLQSIVETNFASRQVLG
                     GATAISNYNNAYILHTAPILLIGTAISTAAFPRLNQRLSQGRPDLFRKDFLMILRAMI
                     WITLPVIIICYFARGYLARLIFTNNNADIALIFGFLTAAIFFRVMYAIMSRWFYAQKD
                     TKTPLFVSIFTISFNIFLAWKLSQPSAYGVAGLALAQSIVAGTEVVILAVIMLFRDRG
                     LFNMAFWGGVIRIISVSGFSVVAGFIMISLYPLGINDTGIITLGGKLALIAAVVFGVH
                     IAMSALFGLEEVRPFFFRVKKLILKPIRLEY"
     gene            complement(4778..5467)
                     /locus_tag="VFH39_04880"
     CDS             complement(4778..5467)
                     /locus_tag="VFH39_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925139.1"
                     /translation="MVLRHSKTYYNRGSGNCFAFVASVANNSVINVGVHMPANTLVAE
                     DLFDAHRTILQAVAAEIEFEHEYRPHPFNAQRALEANNFTSEMLHTAPVEKLLSMGRT
                     LLVGELRNQAIRNHDRKKDDLWSNTMDARLRLEELESELAGQVVLVEAVEEGSRPIEH
                     SRRIPLLGARGFIAVNRIDKMAGRLGLSRQVGNNPTITFGERGSLISHRYETHLLDPQ
                     TLEPLVSVEFL"
     gene            5453..>6690
                     /gene="lepA"
                     /locus_tag="VFH39_04885"
     CDS             5453..>6690
                     /gene="lepA"
                     /locus_tag="VFH39_04885"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6925140.1"
                     /translation="MTQDHIRNFCIIAHIDHGKSTLADRLLELTGTVQARDMKAQLLD
                     KMDLEREKGITIKLAPVRMEHKGYELNLIDTPGHVDFSYEVSRSLQACEGALLVVDAS
                     QGIQAQTLANVYLAMAADLHIIPVLNKIDLPAADVERVSAEVINLLGCKQEDILLISA
                     KTGKGVEAVLDAIIEQVPAPKPSAETKTRALIFDSYYDDYRGVILYVRVFEGAIAKNA
                     QIKMMATAANGLALEVGALKPDMTPGQQLGAGEIGYIVTNLKSTREAKVGDTITLTGQ
                     PATTALPGYKEVLPFVYAGFFPESNEYYQELKDAIEKLSLSDSALQFAPENSPVLGFG
                     VRIGFLGLLHMDIVRERLEREYDLELVVTNPSTDYQVSLLSGDELDIKSAADLPDPAK
                     IKEIREPWVNGEIIVPQDYVG"
BASE COUNT         1452 a         1977 c         1873 g         1388 t
ORIGIN      
        1 ccaaatgaca tgtttatagg cgggcgcctt tgtggtccag ccgcaagctg tcccatggga
       61 agcaatgacc gctttctcga aacgcacggc cttgccgcca ccggcagtcg gatcgttaac
      121 gaccgtggcc gggctggcaa cggagccgct ttcgggctgt accgcgaccg ctggagtgct
      181 ggcgtgggaa tggagtagcc aggcggtacc tatcagaccg aatatcagca caaagctcag
      241 caaggtggaa aggtgcggga gccgctgtag gcgaagggaa ggcatggtct catataacct
      301 ttagggtttt cctcattgta atacaaacgc agccggatgc taatcgccag acctgtagcg
      361 ggtattgcgc cctaacataa ttgttgccac gaaaagcgag cgcgaggcat ccgccgaaac
      421 ccttatgttt agtctgtttt taggtctgaa aaagtgagaa aatggtagct aagcctgcct
      481 gacaggcaga ttacgctatg gcatcaatac tttctcgccc tccgaaacag ccggaacacg
      541 acgacagcgg cgtcgtactg ctgttgcagg ttccccgcac caatgacaaa aaagaactgg
      601 cagccgagca gatgttcgcc agtctgcacg gtcttctgac cttgccgagc aataagttca
      661 tgcagccgac gatgcgcgag cgcctcagct tcgaaatagc cgtcatcggt aagcgcatcg
      721 gcttttacgt gtgggtgccg aattatttga aaagcttcgt cgaggaacag atatacgcgc
      781 agtatccaaa tgtgcagatc agcgaggtgc cggactatac catgacgccg gacggcggct
      841 tcacgacccg tctggtgacc gaaatgaaac tgaccgccgc cgacccgctg cccatcaaga
      901 cgttccagag cttcgaggtc gacccgttgg cggccataac agccacgctg gccaagttcg
      961 aagagagcga agaggcctgg atacagctgg tcatccgccc ggcgaccagt aactggcaca
     1021 agaagagtga gcgttacatc gccggtttac gggctggcaa gagcaacagc atgagcggag
     1081 tgttgggcgc gctgtgggcg ccgccggaat acaagagtga aggcgccaag ctgaccgatt
     1141 acgagcagac ccgggccggc ggcgctgaag agaagagcca gaagctggcc ttcgaaacca
     1201 gcgtgcgtat cgtgtatcgc ggtaacgtgc cggcgcagca ggcacgcctg cacatgcaga
     1261 gcatcgtcgc cagttacaag cagttcaaca ccacctatct gaacggtttc gcagccaagc
     1321 gggtcatgga cgatccgtat ctgctggcac tgtatcgcga ccgacgcttc ggcaaaagcg
     1381 gcgctatcct gaacatcgag gaagtcgcca ccatgtatca cctgccgcac accaccgtgg
     1441 agacgcccta catcttgtgg gcggccagtc agacggccga accgccggcc aacctgccgg
     1501 tcgtaacctc cacctacaat ccgaacatca gccctattgc cgccaccaat ttccgcggcc
     1561 acaacactat gttcggattg cccagaagcg accgcggccg gcacctgtac atcatcggcc
     1621 agactggcgt cggtaaatcc ggtatgctcg agctgctgac catctcggac atctacagcc
     1681 cgttcggctt tgcggtcatc gacccgcacg gcgactacgc gctgaatatc ctgcgccgca
     1741 ttccagcgga gcgcgccgct gacgtcattt atttcaaccc ggccgatacc gactacccga
     1801 tcgccttcaa ccccatggaa gtgaacgacc cgaagctaaa gacgcacacc gtgagcgagc
     1861 tgatcggtgt gctcaagcgc atgttcgaaa gttggggacc gcgcctggag tacatcctgc
     1921 gctacagctt gctggctctg ctggattatc cggatgccac catgctggac attacccgta
     1981 tcctgaccga caaacgattc cgtatggacg tgctcaagta cgtcgaggac ccggtggtca
     2041 aacagttctg gacggtggag ttcgctagct ggaatgagaa gttcgcgacc gaggccgtgg
     2101 cgcccgtgct taataaggtc ggtgccttca cggccaaccc gctggtgcgc aatatcatcg
     2161 gccagccgaa gagcagcttc aacatccggc acatcatgga ccagcgcaag attctcatcg
     2221 tcaacctaag ccgaggtctg gtcggcgaag acaacgccgc cttgctcggc gcactgctgg
     2281 tcaccaagat ccagctggcg gctatgagcc gggcggatat accggccgag gagcgcagcc
     2341 cgttttacct gtatgtcgac gaattccaga acttcgccac cgacagtttc gcggtcatcc
     2401 tcagcgaggc ccgcaagtat gggctgaacc tgacggtcgc caaccagtac atcgcgcaga
     2461 tgagcatgga ggtcaaggac gcggtgttcg gcaacgtggg tagtatcatc gctttccgga
     2521 tgggtgccga tgatgcccgc ggcatgctgc gctacttcga accgaaattc gaggagtacg
     2581 acctggtcca tatgcacaac cggcacttcg tcatcagctt gactatcgag ggcgagaaag
     2641 tcccggcgtt ctctgctata tcacttaatt tacctccgca gcaggaggac cacagtgcgg
     2701 tcatcgtgga gcgttcgcgg gcgcagtacg cggtcagccg ccaatacgtc gagcggtatg
     2761 tgcaggagcg ctacctgacc gactcaacgc cggaagcaaa aaaaatgccg gccgcaaatc
     2821 cgattaccgc accggcagct aaaccgaacg tgtcacgcgc agccggcgca tccgatcaga
     2881 aagcggcggt cctgccgcag ttgatgaagg ccatccaccg tgatgtcgca cccggcgacc
     2941 agccggcttc cgaaccgccg gccaagccga aacgcaaacg tacccgcaaa cgcaaaaaga
     3001 agacagctgc ggcaccgccc tctgatgccg gtgcgagcac tcaggagctg cagggcagtg
     3061 gtgagatcag gcttaagtag gggcttcctg gtgggtatgt atttctctgc tgctttgcgg
     3121 gtgccctccg gctccaaaag ccgtatactg gatggtgacg aatgaatccg accgctccag
     3181 ccaccacacg gaaaagccga cgggtaccgc ttgcaaatgt ggccgtgctg ctcattgcta
     3241 cctccatgtt cggccaggtg ctgggctttt tgcgcaccaa gctcattaac gctaacttca
     3301 acacaccgca cacgccggtc ggccaaaatg ccggcgtcta ctttgcggcc ttcagcattc
     3361 ctgacttttt cttctttacc attgctgccg gcgcgcttgg cgtcgccgtt atgccttact
     3421 tgtcggaccg tttgcacaag ggagaccgca agggtgtgtg ggagctgtcg gccagcctgc
     3481 tcaacttcct ggctatcatc atggccgggg tggcgcttgt tatcatcgtg tttgccaagc
     3541 cgctcatcca ccatgtggtg gcaccgggac tgacgccgga acagctcaac aacgccgcca
     3601 tgctcatgcg tctgctggct ctcaacccgc tgctgttcac cgtgtccggt atccttacga
     3661 gcgcgcagca gaccatgggc cggttcttct tttatgccat agctccgctg ttttacaacc
     3721 tctgcattat catcagcatc tatgcgttcc gcaataacat cggcatcgta ggcttaggta
     3781 tcggtgctct cataggtgcc attctgcagt tggtggtcgt agccttcgga tcgatgcgct
     3841 tgagcttcca ctggcacccg cgcatcatgt ggcggagcgg ggaattccgc tccatgctca
     3901 agcagctgcc gccgcgctca ctcgaccagg gtatggacca actgcagagc attgtcgaga
     3961 cgaatttcgc gtcgcgccag gtactcggtg gtgccacggc catcagcaac tacaacaacg
     4021 cctacatatt gcacacggcg ccgatcctgc tcatcggtac tgccatttct accgccgctt
     4081 tcccacggct gaaccagcgc ctgagccagg ggcgcccgga cctgttccgc aaggacttcc
     4141 tgatgattct gcgggccatg atctggataa cgctgccggt catcatcatc tgctacttcg
     4201 cgcgaggcta cctggcgcgt ctcatcttca ccaataacaa cgccgacatc gcccttatct
     4261 tcggcttcct gaccgcggcc attttcttcc gggtgatgta cgccatcatg tcccgctggt
     4321 tctacgccca aaaggacacc aagacgccgc tgttcgtctc tatcttcacc attagcttca
     4381 acatcttcct ggcctggaag ctgtcgcaac ccagcgccta cggcgtagcc gggctggcac
     4441 tggcgcagtc catcgtggcc ggtaccgagg tggtcatcct ggccgtcatc atgctcttcc
     4501 gcgaccgcgg cctgttcaac atggccttct ggggtggcgt catccggatc atatcggtca
     4561 gcggcttcag tgtggtcgcc ggcttcatca tgatttcgct gtacccgctg ggcatcaacg
     4621 acaccggtat catcaccctt ggcggtaagc tggcgctcat cgctgccgtg gtcttcggcg
     4681 tgcacattgc tatgagtgcc ctcttcggcc tcgaagaagt ccgcccgttc ttcttccggg
     4741 taaagaaact tatcctcaaa ccgatccggc ttgagtacta gagaaattcg acgctgacca
     4801 gcggctcaag agtctgtgga tcgagaaggt gtgtttcgta tcgatggctg atgaggctgc
     4861 cacgttcccc aaaagtaata gttgggttgt tcccaacctg acgagacaag cctaaccgcc
     4921 cggccatctt atctatgcga ttaactgcga tgaagccccg cgctccaagc agcggtattc
     4981 ggcgggaatg ctcaatcggc cgacttcctt cttcgacagc ctccactaat acgacttgtc
     5041 cggcaagctc tgattccagc tcctcgagtc taagccttgc atccatcgta ttcgaccaca
     5101 ggtcatcctt ctttcgatca tggttgcgga tggcctggtt ccgtagctca cccacaagaa
     5161 gtgtacggcc catgctgaga agcttttcga ccggagctgt atgaagcatt tcgcttgtga
     5221 agttgttggc ttcaagcgcc cgctgggcgt taaacgggtg gggtcggtat tcatgctcga
     5281 attctatctc tgctgctacg gcttgcaata ttgtccgatg ggcgtcaaat aaatcttccg
     5341 ctacgagcgt attagctggc atgtgcactc ctacattaat gacgctatta ttcgctacgc
     5401 ttgccacaaa tgcaaaacaa ttgccgctac ctctgttata ataggtcttc gaatgacgca
     5461 ggaccatata cggaattttt gtattatcgc gcatatcgac cacggtaaat cgaccctggc
     5521 cgaccgtttg ctggagttga cgggcacggt gcaggcgcgc gacatgaagg cgcagttgct
     5581 ggataagatg gatctggaac gggagaaggg catcaccatc aagctcgccc ctgttcgcat
     5641 ggagcacaag gggtatgagc ttaacctcat tgatactccc ggacatgtcg atttctcgta
     5701 tgaagtgtcc aggagccttc aggcctgcga aggggcctta ctagtggtag atgcttctca
     5761 gggtatccaa gcccagacac tggccaatgt gtacctggca atggccgccg acctgcacat
     5821 catcccggtt ttgaacaaga tagacctgcc ggcggcggac gtggagcggg tgagcgccga
     5881 agttatcaat ttgctcggtt gcaagcagga agacatactg ctcatttcgg ccaagacggg
     5941 caagggagtg gaagcggtgc tggatgccat catcgaacaa gtgccggccc cgaagccgtc
     6001 ggcggaaacc aagacgcgcg cacttatctt cgacagctac tacgacgatt accgcggcgt
     6061 gatcctgtac gtgcgggtgt tcgagggtgc cattgccaag aacgcgcaga taaaaatgat
     6121 ggccaccgcc gctaatgggc tcgccctgga agtgggcgcg ctcaagccag atatgacacc
     6181 aggccagcag cttggggccg gcgagatcgg ctacatcgtc accaacctca agagcacacg
     6241 cgaagcgaag gtgggcgaca ctatcacgct caccggccaa ccagcaacaa cagcattgcc
     6301 cgggtataaa gaagttttgc cgttcgtgta cgccggtttc ttcccggaaa gcaacgaata
     6361 ctatcaggaa ctgaaggacg cgattgaaaa gttaagcctg agtgattcag cgctgcagtt
     6421 cgcacccgag aattcaccgg tgcttggctt cggtgtacgt attggtttcc tgggactatt
     6481 gcatatggac atcgtgcgcg agcggctgga gcgggaatac gacctggagc tggtggtgac
     6541 caatccgtcg accgactacc aggtgagcct gctgagtggc gatgagctcg acatcaaatc
     6601 cgcggccgac ctgccggacc cggctaaaat caaagaaatc cgcgaaccgt gggtgaacgg
     6661 cgagataatc gtgccgcagg attacgtcgg
//
LOCUS       DASSFD010000201         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_730802, whole genome shotgun sequence.
ACCESSION   DASSFD010000201 DASSFD010000000
VERSION     DASSFD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730802"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFH39_04890"
     CDS             <1..344
                     /locus_tag="VFH39_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925141.1"
                     /translation="GDQVAMINRFFSENGDLYIGFDPDSESSLKAHNHYLALMADRRR
                     NSSSADEREEVRQSIRFFRLEHFIGRVIAWQETAPDAAGPQGQAAEAYDESVIPYPTK
                     AADDDGLRTAS"
     gene            365..934
                     /locus_tag="VFH39_04895"
     CDS             365..934
                     /locus_tag="VFH39_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET6925142.1"
                     /translation="MAFEDYKTKQSVVIVYTGDSKGKTSASLGLMVRALGNRWNVAFI
                     QFIKYWGVGEHVFIRDIMPLYKDQLYFYKGGKGFYRAGELSEEFVSEEKHKQAAGETY
                     DAAYKAATSGAYDLVICDEINNAVHDGLLGEEQLKSLITDRDHQTSLCFTGRNFPPDL
                     LELVDIATDMKKIKHHFDDKFLANKGIDF"
     gene            962..2023
                     /locus_tag="VFH39_04900"
     CDS             962..2023
                     /locus_tag="VFH39_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925143.1"
                     /translation="MSAEFLPDPTPTPAYGGSALHPVPEARRLFLFRRPRQLEPHPLH
                     PEIMRWNPSAERAAAVVRGFLRTAGAYSGDFPEVHILDTHPDASAMAEEFEANGITSE
                     DGVAVVGGDGTHSDANEAARLAGFKGSMLAIAAGNACDLTHMLLRHPHIKDPARAVME
                     SRQAKLYPLRVAIGSLVLNSEAYFSAGEATYQVGREVADPVWRARTENMHSVERFRQE
                     TLMTKRKLPDARRQPLWLEDNRGRRLISDVIFPNGWRMAKQIRFSGVKLLEPGYGRLE
                     LPKATVASVLTTFGKAGIGAFERCEADKTYECTVSSIDGSPVHAQRDGEIFEFPSGST
                     LTVGVQEDYTNQATTRRTA"
     gene            2065..>2293
                     /locus_tag="VFH39_04905"
     CDS             2065..>2293
                     /locus_tag="VFH39_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925144.1"
                     /translation="MVKHETATEVLPVIHEFIATTGSLSARQLYLLELTKPLVSKELY
                     AEHDRHIEQEIARNVTLLSDICAGLQRNGIDI"
BASE COUNT          546 a          696 c          640 g          411 t
ORIGIN      
        1 aaggggatca ggttgccatg ataaaccggt tcttcagcga aaacggcgat ctctacatcg
       61 gcttcgaccc ggacagcgag tccagtctga aagcccacaa tcactatctg gcactgatgg
      121 cagaccgcag aaggaacagc agctccgccg acgagcgtga agaggtccgg caaagtatcc
      181 ggttcttccg ccttgagcac ttcataggtc gcgtcatagc ctggcaggaa accgcgccgg
      241 acgctgcagg cccccaaggg caagcagccg aagcctatga tgagtccgtt ataccgtacc
      301 cgacaaaagc cgcggatgac gacggcttgc gcaccgccag ctaatcaccg atactaaagc
      361 tgtcatggct tttgaggatt ataagaccaa gcagtcggtc gtcatcgtct ataccggcga
      421 cagcaagggc aaaaccagcg cctccttagg cctgatggtg cgtgctctcg gcaaccgctg
      481 gaatgtggca ttcatccagt tcatcaaata ctggggcgtc ggcgaacacg tcttcatccg
      541 cgacattatg cccctgtaca aagaccagct gtatttttac aagggcggca aaggcttcta
      601 tagggccggg gagctcagcg aggaattcgt aagcgaagaa aagcacaagc aggcggccgg
      661 agaaacgtac gatgccgcct acaaggctgc caccagcggc gcatacgacc tggtcatttg
      721 cgacgagatc aacaacgccg tccacgacgg cttactcggc gaagaacagt taaaatccct
      781 gataacagat agagaccacc agaccagcct gtgcttcacc ggccgcaact ttccgcccga
      841 cctgctggaa ctggtagaca tagccaccga tatgaaaaag atcaaacacc atttcgacga
      901 caagttcctg gccaacaagg gcatagattt ttaaccatta acatggtatt gtagactttg
      961 catgtctgca gagtttttgc cggaccctac accgaccccc gcctatgggg gatccgcgct
     1021 gcaccccgtc cctgaagcgc gccggctttt cctgttccgc cggccgcgcc aactcgagcc
     1081 gcatccctta cacccggaaa tcatgcgttg gaatcccagc gccgaacgag ccgccgcggt
     1141 agtgcggggg tttttaagga ccgccggggc gtactccgga gattttccgg aggttcatat
     1201 actcgatacc cacccggacg ccagtgcgat ggcggaggag ttcgaagcca acggcataac
     1261 cagcgaggac ggcgtggccg tcgtaggagg tgatggcacg cacagcgacg ctaacgaagc
     1321 cgcccgttta gccggcttca agggaagcat gctggccata gccgccggca atgcctgcga
     1381 ccttacccac atgctgctac gccaccccca cattaaggac ccggcccgtg ccgtcatgga
     1441 aagccgccag gcaaagctgt accctctccg ggtcgctatc ggcagcctgg tgcttaacag
     1501 tgaggcctat ttcagtgccg gcgaagcgac atatcaggta ggcagggaag tcgccgaccc
     1561 cgtctggcgc gcccggacag aaaacatgca ctctgtcgag cggttccggc aggaaacgct
     1621 tatgacgaag cgcaaattgc cggatgcccg ccggcagccg ttgtggctcg aggacaaccg
     1681 cggccggcgg ctgataagcg acgtcatatt ccctaacggg tggcgtatgg ccaagcagat
     1741 acgattcagc ggcgtaaaac tgctggaacc cggctacggc cggctcgagc ttcccaaagc
     1801 taccgtagct tcggtgctca ccaccttcgg caaagcgggg atcggggcct tcgagcgctg
     1861 cgaggcggat aaaacatacg aatgtaccgt ctccagtatt gacggcagcc cggtccacgc
     1921 ccagcgcgac ggcgaaattt ttgaattccc gagcggctcc acgctcactg taggcgtcca
     1981 ggaagattac accaaccagg ccacaacccg gcgcaccgcc tgaaagcgct cgacaccgcc
     2041 gtccaaagct gttattgttg cgctatggtt aagcatgaga ccgctaccga ggttttaccg
     2101 gtcattcatg agtttatcgc aacgaccggc agcctttccg cccgccagct gtatctgctc
     2161 gagctgacca agccgctggt atcaaaagag ctgtacgccg agcacgacag acacatagaa
     2221 caggaaatag cccggaatgt gacactgctg tcggatatat gtgccggcct gcagcggaac
     2281 ggtatcgaca taa
//
LOCUS       DASSFD010000202         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1621228, whole genome shotgun sequence.
ACCESSION   DASSFD010000202 DASSFD010000000
VERSION     DASSFD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621228"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFH39_04910"
     CDS             complement(<1..492)
                     /locus_tag="VFH39_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6925145.1"
                     /translation="MNPRKIVKKLIPKDVFTAIEPYGHLVEAVFFNVIHGFPGRGMKV
                     IGVTGTNGKTTTTFMIHRMLVEAGYKTGLMTTVAWGIDNDIESQVQHYTNVPVPELMR
                     RLKKLKAANIDWLVLEITSQALAQNRAWGVPFSVAVMTNVTHEHLDYHKTFERYRDAK
                     RKLF"
     gene            complement(556..>2450)
                     /locus_tag="VFH39_04915"
     CDS             complement(556..>2450)
                     /locus_tag="VFH39_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925146.1"
                     /translation="ESKMPTASILKKRPEEPKRYGLIDAKDEDWSTRQAIKRDGVFSK
                     AEYKQKYGEYRPAVKQVAEAAPRKAVNTEKQPKAPKNNGGKGNGKPESAEKKSNGDNK
                     RAKQAKGNSSVAPETSSKPSDAEAEQLAQPEPTTEEELTAEQKADRDYKAFLEGLSDS
                     EYADVEKLNVYIGKNHLDGSSFKTNDLFGNALGTTQEGRVLDFAKYKEALQHMAATGV
                     LVSDKDTHNNGTPRTYRISPELLEQLRNQATSAQPVGETAATQVAPESTEPAASTSRE
                     QVAAANTEPLQTAATETVPAPTADAETAIDGLSPEQQDKISIWLSEYNSQLKPGQGVS
                     LRRIRSAMKRDGIKVKAETLDKLLRTFEDPDSLIRYESYENHGVPYYRRPNEPSRSTA
                     RTNAAEQTTAVQAPVQTAEATREPVASPAAEHVADDKMQAFFNQLPDSRERTDFKYIH
                     MFLMRRLRKGQFEGGTLDDWFEHERKGSTITKEHAQRYLDLLAADPSVPIVKNTDGSN
                     GSTYRLDGSAVAQTQEAPRILGFDAEEETPRQRQPESRLSRRERVRRLGQRAVAFDRR
                     SQELVTTFGKKVYKHEPWYKARNLAKEDGRQVAEERAQRERDEAERERIERERRAAEA
                     SLVTNP"
BASE COUNT          436 a          714 c          667 g          633 t
ORIGIN      
        1 gaacagcttg cgcttggcgt cacggtaacg ctcgaacgtc ttgtgataat cgaggtgttc
       61 atgtgtcacg ttcgtcatga cggccacgct gaacggcacg ccccaggccc ggttctgggc
      121 cagcgcctga ctggttatct ccagcaccaa ccaatcgatg ttggccgcct tgagcttttt
      181 gagccgtcgc atcagctccg ggaccggcac gttggtgtag tgctgcacct ggctctcgat
      241 gtcgttgtcg ataccccagg ccacggtcgt catcagcccg gtcttgtagc cggcctccac
      301 cagcatccgg tggatcataa aggtcgtggt ggtcttacca ttggtgccgg tcaccccgat
      361 caccttcatc ccccgcccgg ggaaaccgtg gatcacgttg aagaataccg cctccaccaa
      421 atgcccgtac ggctcaatag ccgtaaacac atccttaggt atcagcttct taacaatctt
      481 ccgcggattc atctgcatcc aagtataccg ataaaccgtt acgaatagaa aaccggccgc
      541 ggtgtgcggc cggtcctacg ggttggtgac taacgatgcc tcggcggccc gacgctccct
      601 ttctatccgc tcccgttctg cttcatcgcg ctctcgttgc gctcgttctt cagctacttg
      661 gcggccgtct tccttggcca ggttacgcgc cttataccac ggctcgtgct tatatacctt
      721 cttaccaaat gtcgtgacca gttcctggga tctgcggtca aacgcaacgg cccgttggcc
      781 taaccgtcgg acacgttcac gacgacttag acgactctcc ggctggcgct ggcgtggcgt
      841 ctcctcttcc gcgtcaaagc ctaaaatgcg aggggcttcc tgggtctgcg caactgcaga
      901 accgtccagg cggtacgtgc ttccgttgct accatcagtg ttcttcacaa tcggcaccga
      961 gggatcggca gccaataggt ctagatatcg ctgggcgtgc tccttggtaa tagtggagcc
     1021 cttccgctcg tgctcgaacc agtcatcgag agtgccaccc tcaaactgac ccttacgcaa
     1081 gcggcgcatc aggaacatgt gaatatactt gaagtcggtg cgctcacgag agtcaggcag
     1141 ctggttgaag aacgcctgca tcttgtcgtc cgctacatgc tcagccgcgg ggctagcaac
     1201 cggctcacgg gtagcttctg cggtttggac aggtgcctga acagcagtag tctgctcagc
     1261 ggcgttcgtc cgtgccgtac tacggctggg ctcattcgga cgacggtagt aggggacacc
     1321 gtgattctca tagctttcgt aacggatcaa ggagtccggg tcttcgaacg tacgaagcag
     1381 cttgtcgagg gtctctgcct taaccttgat gccgtcccgc ttcatggcac tacggatgcg
     1441 acgcaggcta acgccctgtc caggcttcaa ctggctgttg tactcactca accaaatgct
     1501 gattttgtct tgctgctccg ggctcagacc gtcaatggcc gtttcggcat cagcggtagg
     1561 cgccggtacg gtttcggttg cagcggtttg cagcggctcg gtgttagcgg cagctacttg
     1621 ctccctagag gtagaagctg caggctcggt gctctcggga gcaacctgag ttgccgcagt
     1681 ttcaccgacg ggttgggcgc tggtggcttg gttacgtaac tgttcgagga gttcgggaga
     1741 aatcctgtag gtgcgaggcg taccgttatt atgcgtatct ttgtctgaga ccagcacgcc
     1801 agtcgcggcc atgtgctgca atgcctcttt gtacttggcg aagtcaagga cacgaccttc
     1861 ctgagtggta ccgagagcgt tgccgaagag gtcgttggtc ttaaacgatg agccgtcaag
     1921 gtggtttttc ccgatataaa cattaagttt ttctacatcg gcatactcgc tatcacttaa
     1981 cccctcgaga aatgccttat agtcccgatc agctttctgc tcagccgtaa gttcttcttc
     2041 agtggtcggc tcaggttggg ccagttgttc ggcttccgca tcactaggct tactggaagt
     2101 ctccggagcg acagagctgt tgcccttcgc ctgcttggca cgcttattgt caccgttact
     2161 tttcttttca gcgctttcgg gcttgccgtt gcctttgccg ccgttgtttt taggcgcttt
     2221 cggttgcttt tcggtattaa cagctttgcg tggagccgct tcagctacct gcttaacagc
     2281 cggccggtat tcgccatact tctgcttgta ttctgctttg ctgaagacac cgtcacgctt
     2341 gatagcctgt cgtgtgctcc aatcttcgtc cttagcatcg atcaaaccgt aacgcttcgg
     2401 ttcctcgggg cgcttcttca ggatcgaggc tgttggcatt ttgctttcca
//
LOCUS       DASSFD010000203         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1668089, whole genome shotgun sequence.
ACCESSION   DASSFD010000203 DASSFD010000000
VERSION     DASSFD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668089"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFH39_04920"
     CDS             <1..516
                     /locus_tag="VFH39_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925147.1"
                     /translation="QSYLQSATDDDHLFTKIVNAPFSDPVPAAFVFLGIVVLLQVNKR
                     TKTIDRVALSETDLARNTTEVSVVPFHDIAIPLDHYENIIAHAIASGEPQDTTDWKFL
                     FEPALSPEQARINQASAGIAYSCVFPLTARDGGAMIFSYFQYQHDIDSKQMEFMRRYS
                     ALVDAALAKKA"
     gene            complement(513..1844)
                     /locus_tag="VFH39_04925"
     CDS             complement(513..1844)
                     /locus_tag="VFH39_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925148.1"
                     /translation="MERGAEAEPPVEVNTEQMRGVEPSRPRLTAHQHHYLVLGQLYGE
                     SNNASDPVMRAAFTAVCDELHTNKKLYEALLVYSQANRDDSVSAATRKIYSSLQQFVI
                     PLPQRNIVIADEHAGMRVPTFPEGFEDPARWSTLIRSITGNIRSLSDFRLTLIDKELQ
                     SNVVSRYIPPRVAAQLFGLSNLDIYSGGASFGIVEKMEKLYKPGRYRLPLTISVGQLG
                     VNGRWDFTPSDTLTGDYCRLLNAEYKVGQIIAGDIDPVLDPLTATYAYSNSFRPSEFG
                     NRQYRKIVDRLLVRHVPGMRYTKGGDKGDFTHPKHVDYIREAFPSWFNRPGRKVQFLT
                     SSYIIHEEHEALRQAEANARDLAGDEGVIFWMDFCHRSPNGELNFIGDKWQDGDYNLY
                     ALDMQNQSAGKQHIFTFREGRAAQVAPGEDLKRKMNAYGGRLVAGMMRDAA"
     gene            complement(1915..2616)
                     /locus_tag="VFH39_04930"
     CDS             complement(1915..2616)
                     /locus_tag="VFH39_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET6925149.1"
                     /translation="MNSKLGVLIDFGIIVSQKVIDSFPLGIVNSHFSLLPEWRGADPI
                     TFSILSGQKETGVSLMLLTAGLDEGPLLANSTPVTIASDETTASLTDTLIAESDANLR
                     VVLPLYVSGTAKPIDQFAIAEQVPGYPSKATYSRKLTKDDGILDFTKPADQLEREVRA
                     FAEWPKSRATIGGKEVVITKAHVMDGAGKPGESWKDGKQFGFYTTSGIFVIDALKPAG
                     KGEMTAAAFLNGYSL"
BASE COUNT          536 a          764 c          788 g          610 t
ORIGIN      
        1 cagagctatc tccaatcggc taccgacgac gaccacctgt ttacgaagat cgtgaacgcc
       61 ccgttcagcg atcctgtgcc ggccgcgttc gtgttcctgg gcatcgtggt gttgttgcag
      121 gtgaataagc gcaccaagac catcgaccgg gtggccctga gcgaaaccga cttggcccgc
      181 aatacgaccg aagtgtcggt ggtgccgttc catgacatcg ctatcccgct agaccactac
      241 gagaacatca tcgcgcacgc catcgcctcc ggcgaaccgc aggacacgac cgactggaaa
      301 ttcctgttcg agcctgccct gagcccggaa caggcgcgta tcaatcaggc cagtgcgggc
      361 atcgcctaca gctgcgtctt cccgctgacg gcccgcgatg gcggggcgat gattttcagc
      421 tacttccaat accagcacga catcgacagc aaacagatgg aattcatgcg ccgctacagc
      481 gcgttggtcg acgccgccct cgccaaaaaa gcctaagccg cgtcgcgcat cataccggcg
      541 accagccggc cgccatacgc attcattttg cgcttcaggt cttcgccggg cgcgacctgc
      601 gcggcccggc cttctcggaa ggtgaaaatg tgctgcttgc cggccgattg gttctgcatg
      661 tcgagtgcgt atagattgta gtcaccatcc tgccacttat ctccaatgaa gttaagttca
      721 ccgttcgggc tgcgatggca gaagtccatc caaaaaataa ccccctcatc gcccgccagg
      781 tcacgggcgt tggcctccgc ctgccgcagt gcttcgtgtt cctcgtggat gatgtaactg
      841 ctggtcagga actgtacctt gcgacccggc cggttaaacc agctggggaa ggcctcgcgg
      901 atgtagtcca catgtttggg atgcgtgaag tcacccttgt ctccgccctt ggtgtagcgc
      961 atgcccggca catgacgtac gagcagccga tcgacgatct ttcggtattg ccggttgccg
     1021 aattcggacg ggcggaacga gttgctgtag gcataggtcg cggtcagcgg gtccagcacg
     1081 gggtctatgt cgccggctat gatctgaccg accttatact ccgcgttcag cagccggcag
     1141 taatcacccg tcaaggtgtc ggacggagta aagtcccacc ggccattgac gcctaactgt
     1201 cccacgctga tagtcagggg gaggcgatag cgtccgggtt tatagagttt ttccattttt
     1261 tcaacgatac cgaagctggc gcctccactg taaatatcca agttactgag gccgaacagc
     1321 tgagcggcta cgcgcggtgg tatgtagcgg ctcaccacat tgctctgcag ctctttgtct
     1381 ataagggtca agcggaagtc cgacagggag cgtatattgc cggtaatact gcgtatgagt
     1441 gttgaccagc gtgccgggtc ttcgaagcct tccggaaaag tcggtacgcg cataccggca
     1501 tgctcgtcgg cgataacgat gttgcgctgg ggcaacggga tgacgaactg ctgcagactg
     1561 gaatatatct tgcgggtagc ggccgagacg gaatcgtccc ggttagcctg cgagtacacg
     1621 agtagtgcct catacagctt cttgttggtg tgcagttcgt cacagaccgc cgtgaaggca
     1681 gcgcgcataa cgggatcgga cgcgttgttg gactcgccgt agagctggcc cagcactaaa
     1741 tagtggtgct ggtgtgcagt gaggcgcggg cggcttggtt ccacgccgcg catctgctcg
     1801 gtattaactt cgactggtgg ctcagcctca gccccacgct ccatatccat gctcacactg
     1861 tatcatcttt acaacagatt gagaagatgg gagagtatat aaaaatcagt gctcttagag
     1921 ggagtagccg ttcaggaagg cggcggcggt catctcgcct ttgccggccg gtttgagggc
     1981 gtcgatgacg aatataccgc ttgtggtata aaaaccgaac tgtttgccat ctttccagct
     2041 ttcgcctggt ttcccggcgc cgtccatgac gtgcgccttg gtgatcacga cctctttgcc
     2101 accgatcgtg gcgcggctct taggccactc ggcaaaagcg cggacttcgc gctccagctg
     2161 gtcggccggt tttgtaaagt ccagaatgcc gtcgtctttg gtcagcttgc gggagtaggt
     2221 agctttcgat ggataacccg gtacttgctc agcgattgca aactgatcga tcggtttggc
     2281 tgtcccgctg acgtaaaggg gtaatactac ccgtaaattg gcgtcacttt cggcaatcaa
     2341 ggtatcagtc aggctggctg tcgtttcgtc ggaggcgata gtgaccggtg tcgaattggc
     2401 cagcagcggg ccctcgtcca gtccagccgt cagcagcatc agactcacgc cggtctcttt
     2461 ctggccggag aggatgctga aggtgatcgg gtcggcgccg cgccactccg gcaacaggct
     2521 gaaatggctg tttacgatgc cgagcgggaa ggagtcgatg actttctggc tcactatgat
     2581 gccgaaatct atcagcacac ctagtttgct gttcaatgtg ttttggtcaa cgatgctgtc
     2641 taaatctttt ttattcgtga ccgtgtggac cgacaggcct cagtgttcgg cgatatcc
//
LOCUS       DASSFD010000204         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1746996, whole genome shotgun sequence.
ACCESSION   DASSFD010000204 DASSFD010000000
VERSION     DASSFD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746996"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..375
                     /locus_tag="VFH39_04935"
     CDS             235..375
                     /locus_tag="VFH39_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925150.1"
                     /translation="MATNKLDDLGENARQTTDDYIDKQKEKVQEQKEASKENAPLDES
                     DD"
     gene            complement(372..833)
                     /locus_tag="VFH39_04940"
     CDS             complement(372..833)
                     /locus_tag="VFH39_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6925151.1"
                     /translation="MPHIHELYDFTVAVFIVHDGKVLLVNHPRYNKWIPPGGHIELDE
                     DPEQALYREIEEETGLKVEILSTKPVVESPETKFILTPNYVDVHEANLPHRHIALTYF
                     ARAASPDFRLSAEHTDMRWFMPDELEKPEYKLSPAVQFYAREALNAAALMR"
     gene            complement(836..1180)
                     /locus_tag="VFH39_04945"
     CDS             complement(836..1180)
                     /locus_tag="VFH39_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925152.1"
                     /translation="MTHHFEKPITLEQARYEWMRERARQLMMADIYTTPPPEVTHQEM
                     EDLRHHTLSTMPAEDTFRDPIQQRLASIVSHPATLYVYAQYLKRETRVIDKMIAESSI
                     VEYPEDGPYQES"
     gene            complement(1386..1461)
                     /locus_tag="VFH39_04950"
     tRNA            complement(1386..1461)
                     /locus_tag="VFH39_04950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1426..1428),aa:Pro,seq:ggg)
     gene            1688..2404
                     /locus_tag="VFH39_04955"
     CDS             1688..2404
                     /locus_tag="VFH39_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HET6925153.1"
                     /translation="MWQETKHGLYKQFNFDNFRDAFAFMTKVAALAERFNHHPRWENE
                     WSVVQIWLITHDVGGKITQQDREMAEAIDAILSGEQVPSEVTADLARQEKIKLFADGG
                     SRGNPGPSASGFVILDMNDKVLLDQGVYLGVTTNNQAEYTALKLGLEAAHKMGAQEVE
                     VYMDSMLVVNQMKGIFKIKNRDLWAVHDAIKQMLHLFKKVTFDHVPREFNKLADAAVN
                     RAMDEELGIRPSSHLEQPEN"
BASE COUNT          562 a          645 c          675 g          545 t
ORIGIN      
        1 ccgggacttc ggtcccgacg ccgccgacaa atgattatct gaatgctaat cattagggag
       61 ccttacagag tgtcgaccgt ggtcggcagt tgcgggcacc cacctggaat tatttcaggt
      121 taatcacgtc ggcacccagc attttttgat tctaagccgc tccgcttgtt agataaataa
      181 cagctcttcc gaactatttg acgcatacta gctgtagtta aatgaggatt acatatggcc
      241 acgaataaat tagacgattt gggcgaaaac gcccgccaga ccaccgacga ctacatcgac
      301 aagcagaagg aaaaggtgca ggaacagaag gaagcttcca aagagaacgc tccgctcgac
      361 gaaagcgatg actagcgcat tagtgcagcc gcgttaagcg cttcgcgggc ataaaactga
      421 acggccggag acaacttgta ctccggcttt tctagttcgt cgggcataaa ccagcgcatg
      481 tcggtgtgct cggcggacag ccgaaaatcc gggctcgcag cacgtgcaaa ataggtgagg
      541 gctatatgcc gatgcggtag gttggcttcg tgcacatcga cataattcgg cgtcagtatg
      601 aatttagtct cgggcgactc cacaaccggc ttggtgctca agatctccac tttcaggccg
      661 gtttcttcct cgatttcgcg gtacagcgct tgctccggat cctcgtcgag ctctatgtgg
      721 ccgcccggcg gaatccactt gttgtaacgc ggatggttca ccagcaaaac cttgccgtcg
      781 tgcacgatga agacggcgac ggtaaagtca tatagttcgt ggatatgcgg catattcagg
      841 attcctgata gggtccgtcc tcgggatact cgacaatcga tgattcggct atcattttgt
      901 cgattactcg agtctcacgt ttaaggtatt gggcgtagac ataaagcgtg gcaggatggc
      961 tcactatgct tgccagccgc tgctgtatag gatctctgaa agtgtcttcg gccggcatcg
     1021 ttgataaggt atggtggcgt aaatcttcca tttcttggtg cgtaacttcg ggcggcggcg
     1081 tagtgtatat atcggccatc atcagctgcc gggcgcgctc gcgcatccat tcgtaccgcg
     1141 cctgctcaag ggtgataggc ttttcaaaat gatgagtcat aagttccccc tttcgtgaac
     1201 catgctagca cagtattggc catgttttcc gtaggcgaac ctacatgcag accggccagc
     1261 tactgcaatc caagcaaaaa gcccggcgct aagccgggcc ctttgcttgg tcggggtgac
     1321 aggacttgaa cctgcgaccc agaacagccc ggcgggctgt tactgtagac tcgaatgaaa
     1381 tgtcgtggtc ggggtgacag gacttgaacc tgcgacctca cggcccccag ccgtacgcgc
     1441 taccaactgc gctacacccc gtcgtcgcag cctgcgcctc tgtctcgttt tgccgcgctg
     1501 cggcaaaagc ttcaactgac ggctacgctg ctcctcccaa aatcgcattc actcgcctgc
     1561 ggctccttgg attgcgattt tggtcttagc ttccttaagc tgcaggtatt ttagcgtatt
     1621 tcgtatctgt tggcgaggtg tcgctacggg tgcgacttct accggcccct catgctaaga
     1681 tgtaagcatg tggcaagaaa ccaaacacgg gctttataaa cagttcaatt ttgataattt
     1741 tcgcgacgcg ttcgcgttta tgaccaaggt ggcggcgctg gcggagcggt tcaatcatca
     1801 cccgcgttgg gagaatgaat ggagcgtggt gcagatatgg cttataacgc acgacgtggg
     1861 aggcaaaatt acccagcagg accgcgagat ggccgaagcc atcgatgcca tcctgtctgg
     1921 tgagcaagtc ccgtcggaag tgacggccga cctggcgcga caagagaaga ttaagctgtt
     1981 tgccgacggc ggctcgcgcg gcaatcccgg cccgagcgcc agcggttttg tgatactcga
     2041 catgaacgac aaggtgctgc tcgaccaagg cgtgtacctg ggcgtcacga ccaataacca
     2101 ggcggagtac acagcgctca aactcggcct tgaagcggcg cacaaaatgg gcgcccaaga
     2161 ggtggaagta tacatggaca gtatgctggt ggtgaaccag atgaagggca tcttcaagat
     2221 aaagaatcgc gatttgtggg cggtgcatga cgcaataaag cagatgctcc acctgtttaa
     2281 gaaagtgacg ttcgaccacg tcccgcgcga gttcaacaag ctggccgacg cggccgtcaa
     2341 ccgcgccatg gacgaggaac tgggcatccg cccaagctcc cacctggaac agcccgaaaa
     2401 ctagctattt tattccgtaa gtgatgt
//
LOCUS       DASSFD010000205         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1889798, whole genome shotgun sequence.
ACCESSION   DASSFD010000205 DASSFD010000000
VERSION     DASSFD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889798"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFH39_04960"
     CDS             <1..284
                     /locus_tag="VFH39_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925154.1"
                     /translation="TVEKPNLEGLQLFKPGSSPANPYGFSGQLAIREQFTMNGEIRQL
                     LETHTQVLSTQEIEAAAIRSGMRTMLQDGMLKVIAGQTSLEEIYRVLGS"
     gene            321..779
                     /locus_tag="VFH39_04965"
     CDS             321..779
                     /locus_tag="VFH39_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6925155.1"
                     /translation="MEALPRMPEPDWESTDEREPTPEELAAEEALFRDLVRQGKYRPT
                     WRSTELQPKPASVTDEKLPDLAAVPWQDKRRWAWAAACRKVDPDMFYGSHSIYAKRPT
                     EIERQAKKVCADCPVQAQCLTWALNARERWGVWGGMTVAEREALTKRASA"
     gene            821..1339
                     /gene="rplS"
                     /locus_tag="VFH39_04970"
     CDS             821..1339
                     /gene="rplS"
                     /locus_tag="VFH39_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET6925156.1"
                     /translation="MESVIQKVNAKFMKSAVVDVRSGDTVKVHQKIREGNKERVQIFQ
                     GLVIRTDRKGSHTSRITVRRIASGIGVEKSFLLHSPLVLKVEVTKRSKVRRNYLTYMR
                     ERTGKSARLQGREFDREAVNTIHDAAAEAEEEKLKEETAKAAEAEAEAKAAEEAEREA
                     KVAEAVAQHDEA"
     gene            1360..1725
                     /locus_tag="VFH39_04975"
     CDS             1360..1725
                     /locus_tag="VFH39_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925157.1"
                     /translation="MPEVPSERGRDFFGSLDESRQTLFRALADRMLAGQMLGTQDIAD
                     IIRGVDYPTSDAGSASSKVLTSYKSRRVLEELGVDVRLGLNDGKRAYYNGAQPGAQAT
                     KQPAHEAGMPEDIPALEAS"
     gene            1722..2285
                     /locus_tag="VFH39_04980"
     CDS             1722..2285
                     /locus_tag="VFH39_04980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET6925158.1"
                     /translation="MSIVGIDEVGRGCWAGPLVAGAVILDKPIRGLNDSKKLSKRQRE
                     KLAAEIQIEAGAIGLGWVSAEELDKVGITTAVKLAMERALKQITTPYDEVIIDGNINY
                     LPAEPRAKAVIKADATVPAVSAASIVAKVARDAYMAQLDDTYAVYGFEKHVGYGTAAH
                     VAALQQFGVSDIHRRSFKPIKALLEAA"
     gene            2282..2626
                     /locus_tag="VFH39_04985"
     CDS             2282..2626
                     /locus_tag="VFH39_04985"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET6925159.1"
                     /translation="MSTTAIGRQAEQAAAEFLRGQGYEILEHNWRTRWCEIDLVARKG
                     GCVHFVEVKYRSQDGQGSGLEYITSAKLKQMRYAAEHWTQQHNWSGAVNLAAVEVCAP
                     DYAISEFVESIY"
     gene            complement(2616..>4096)
                     /locus_tag="VFH39_04990"
     CDS             complement(2616..>4096)
                     /locus_tag="VFH39_04990"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET6925160.1"
                     /translation="RDSIAGVQSVQTPVNHPDEIGSLFDPSIVYAKGGRLLNMLMNYI
                     GQDDFRKGLKAYFEKHAYGNTTGNDLWAALSEASGKDIAAFMDPWLTRSGFPLVTVDQ
                     QGDSVDIKQEHFLVNPEQADHDRIWPVPLLASSPDVPELLASREAQTTLTSREYVRLN
                     EGAVGHYIVRYAQPEHAAALAALAESQELSAPERMMLLNDSSMIAKAGFASFGETLDL
                     LSHYASEASETVWDILSVIIADARRFIDQDLELEADIKRFIRGFIQRQYDRLGWEEQN
                     GEPASDRKLRATIIGLGVYSEHEDILATALDKFETYKADHDAVPSELRSIVLGAAVRH
                     AAAGAFDYLLHEEETTSNPDLKQELLGALSTTRSEAEVATLLGRIKDSTKVRQHDVDT
                     WLVYLLRNRYGRQQSWDWLRDNWSWIEKTFEGDKGYDSFPRYAASAFNTRQLFEEYKE
                     FFEPKAGDVALSRNIKLGVAELASRIAWLERDLTAVRSFFSK"
BASE COUNT          904 a         1105 c         1301 g          786 t
ORIGIN      
        1 caaccgtcga aaagcctaat ctggaaggcc tgcagctatt caaaccgggc agctcgccgg
       61 caaatcccta cggcttttcg ggtcagctgg ccatccgcga acagttcacc atgaatggcg
      121 aaattcgcca gctgctggaa acccacaccc aggtgctctc tacccaggag atcgaagcgg
      181 ccgccatccg cagcggcatg cgcaccatgc tgcaggacgg catgctcaaa gtcatcgccg
      241 gccagacctc gctcgaggaa atctaccgcg ttctcggttc ctaaagggga tcctcagggg
      301 tccatagtat aatgatacgg atggaagcat tgccgcgtat gcccgagccg gattgggaat
      361 caaccgatga gcgagaaccg acgcccgaag agttggccgc cgaagaggcg ctgttccgtg
      421 atttagtccg gcaggggaag taccggccga cttggaggag taccgaactg cagccgaagc
      481 cggcatctgt gacggatgaa aagctacctg atctggcagc ggtcccatgg caagacaaac
      541 ggcgctgggc ctgggctgcg gcgtgccgca aagttgatcc ggatatgttt tacggcagcc
      601 acagcatcta cgctaagcgg cctactgaaa tcgaaagaca ggcaaaaaaa gtatgtgcag
      661 attgtcccgt acaggcgcag tgcctgacat gggcgttgaa tgctcgcgaa aggtggggag
      721 tatggggcgg tatgacggtt gccgagcgtg aggcgctcac caaacgcgcc tcggcatgag
      781 acttgcgtca cagaagtaaa tagtgtaagc taatacggat atggaaagtg ttattcaaaa
      841 agtaaacgcc aagtttatga agtcggccgt ggtcgacgtg cgttctggtg acaccgtgaa
      901 ggtccaccaa aagatccgcg aaggtaacaa ggaacgggtg cagatcttcc agggtctggt
      961 tatccggact gaccgcaagg gcagccacac cagccgcatc accgtccgcc gcattgccag
     1021 cggtatcggg gtcgagaaga gctttttgct gcacagcccc ctggtgctga aggtcgaagt
     1081 caccaagcgg agcaaggtcc gccgcaacta cctcacttac atgcgcgagc gtaccggcaa
     1141 gtcagcccgc ctgcagggtc gcgagttcga ccgtgaggcc gtgaacacca ttcacgacgc
     1201 cgccgccgag gccgaagaag agaagctgaa ggaagagacc gccaaagccg ccgaggctga
     1261 agccgaagcc aaagctgccg aagaggccga gcgcgaagcc aaagtcgccg aagccgtcgc
     1321 tcaacacgac gaagcctaag ccctacggag acgaaagtca tgccagaagt cccatccgaa
     1381 cgaggaaggg acttttttgg ttccctggac gaatcacgcc aaactttatt tcgcgcattg
     1441 gccgaccgta tgctggcggg acaaatgctg ggcacccagg atatcgcgga tataatacgg
     1501 ggagtagact atccgacgtc ggatgcgggg tcggcctcga gcaaagtgct tacttcttat
     1561 aagtcgcgcc gggtattgga agagttaggg gttgatgtgc ggctcggctt aaacgatggt
     1621 aaacgggcgt attataacgg tgcccaacct ggcgcgcagg ctacaaaaca gcccgcccat
     1681 gaagccggga tgcctgagga catacctgcc ctggaagcgt catgagcata gtcggcatcg
     1741 acgaagtagg acgcggttgc tgggccggtc cgcttgtggc tggggccgtg attctggata
     1801 agcctatccg tggcttgaac gactccaaaa aactgagcaa gaggcagcgc gaaaaactgg
     1861 cggctgagat tcaaatcgaa gccggagcaa tcggactagg atgggtgtct gccgaggagc
     1921 tagacaaggt gggtattacc accgctgtca agctggccat ggaaagggct ttaaagcaga
     1981 ttactacacc ctatgatgaa gtcataattg atggcaacat caattactta ccggctgagc
     2041 cgcgagccaa agcggtcatc aaagccgatg ccactgtacc ggccgtcagc gctgccagca
     2101 tcgtcgccaa agtcgcgcgg gatgcctaca tggcgcagct ggatgataca tacgccgtct
     2161 atgggttcga aaaacatgtc ggttacggta cggcggcgca cgtcgcggct ttacagcagt
     2221 tcggcgtcag cgacatccat cgccgctcgt tcaaacctat caaagcctta ctggaggcgg
     2281 catgagtacg acggcaatcg gacgccaggc ggagcaggcg gcagccgagt ttctgcgtgg
     2341 acaggggtat gaaatacttg agcacaactg gcgtacccgg tggtgcgaaa tcgatctggt
     2401 ggcgcggaaa ggcggttgcg tgcatttcgt ggaggtgaaa taccgcagcc aggacgggca
     2461 gggcagcggc ctggaataca tcacgtcggc caagctgaag cagatgcggt acgccgccga
     2521 acactggacg cagcaacaca attggtctgg tgctgtgaat ctcgctgctg tagaggtatg
     2581 cgcaccggat tacgccatca gcgagtttgt agagtctatt tactgaagaa actgcggacg
     2641 gcggttaggt cgcgctcaag ccaggcgatc cggctggcta gttcagccac gccgagcttg
     2701 atgttgcggc tgagtgcaac gtcgccggct ttcggttcga agaactcttt gtattcctcg
     2761 aaaagctgac gggtgttgaa ggcgctggcc gcgtagcgcg ggaagctgtc gtagcctttg
     2821 tcgccttcga acgttttctc gatccagctc cagttatcac gtagccagtc ccaggactgc
     2881 tggcgaccgt agcggttccg gagcagatac accagccagg tgtcgacgtc gtgctgacgg
     2941 actttggtgc tgtctttgat acgaccgagc aaggtggcga cttccgcttc ggaacgggtg
     3001 gtgctgagtg caccgagcag ctcttgcttg aggtcgggat tgctggtggt ctcttcctcg
     3061 tgcaataagt agtcgaaggc gcctgcggcg gcatgccgga cggcggcacc gaggacgatg
     3121 ctgcgcagtt cactcggcac ggcgtcgtga tcggctttgt aggtttcaaa cttatccaaa
     3181 gcggtggcca ggatgtcttc gtgctccgaa tacacgccca agccgatgat ggtggcacgc
     3241 agcttgcggt cgctggccgg ctcaccgttc tgttcttccc agccgagccg gtcgtactgg
     3301 cgctggatga agccgcggat gaaccgtttg atgtcggctt cgagctccaa atcctggtct
     3361 ataaagcggc gcgcatcggc gatgatgacc gacaggatgt cccagacggt ctcactggcc
     3421 tcggaggcat agtgggaaag caggtcgagg gtttcaccga acgaggcgaa gccggctttg
     3481 gcgatcatcg agctgtcgtt gagtaacatc atccgctccg gggcggaaag ttcctggctc
     3541 tcggcaagcg cagctaaggc agcggcgtgc tcgggctggg cgtaacggac gatgtaatgt
     3601 ccgacggcgc cttcgttcag acgcacgtac tcacgggacg taagtgtagt ctgcgcctcg
     3661 cggctggcaa ggagttcggg aacgtctggc gacgatgcca gcaacggcac gggccagatg
     3721 cggtcatggt cggcctgctc ggggttaacc aggaagtgtt cctgtttgat gtcgacgctg
     3781 tcgccctgtt ggtcaacggt gacgagcggg aagccggagc gggtcagcca cgggtccatg
     3841 aaagcggcga tgtccttgcc gctggcctca ctcagtgccg cccacaggtc attgccggta
     3901 gtgttgccgt aggcgtgctt ttcgaagtag gccttaagcc ctttacggaa gtcgtcctga
     3961 ccgatgtagt tcatgagcat gttgagcagc cggccgcctt tggcgtagac gattgacgga
     4021 tcgaacaggc tgccgatttc gtccggatga ttgaccggtg tctggaccga ctggacgccg
     4081 gcgatgctgt cgcggc
//
LOCUS       DASSFD010000206         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_737620, whole genome shotgun sequence.
ACCESSION   DASSFD010000206 DASSFD010000000
VERSION     DASSFD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737620"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..782)
                     /locus_tag="VFH39_04995"
     CDS             complement(201..782)
                     /locus_tag="VFH39_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925161.1"
                     /translation="MKLRKLNTSGVTHTLVLLLVIVGAGILGSYFLVASHADTASILL
                     RPVDWVPFQKIDTNSIGVVKDGSHGNVLLLKADTERGDGSAAVRYNSSKKLRDFLNNH
                     ATQPATVCVVARAEHSSKLSLDLVNMTSSSSATMQKTILLGNKYEKHCYDFKLATKSY
                     LGYSQVDVTDQPNATNLSPLHNIYVDSLTISIR"
     gene            985..1560
                     /locus_tag="VFH39_05000"
     CDS             985..1560
                     /locus_tag="VFH39_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925162.1"
                     /translation="MFEYVGLLFVALMILLLGNTQQLLEYYGFDTSGQVIKQGAHQAI
                     ASGLSKLDGLSFTDRVVTFVVWALIGVVCFSLVQLIGRVYHDFEEEEKVSSNRYVHPS
                     TFRRADFWKQVALDFIVATISLGLLVAGVVLLFAYVLPVALSYSESFLTHASLSHFIN
                     FLVGFFMVYVWSLVLAVIFKYYLHRHRLLAR"
     gene            complement(1563..2801)
                     /locus_tag="VFH39_05005"
     CDS             complement(1563..2801)
                     /locus_tag="VFH39_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925163.1"
                     /translation="MPVRPVPAHRPVHKRHAHQIFVALLLVVLLAVATLGYAAAKPLP
                     APVTITRPVTAQLRGEPALNWPSYGQAAVAVSGFGVLATHGDQTPTPTASIAKVMTAL
                     SVLQRKPLGLHQSGPMITMTQADVDGYNSYLSQDGSVVPVQAGEQISEYQALEGMLLP
                     SGNNMADTLARWAFGSLDDYANYANQFAVEHGWNSLHFAGASGFSSDTTGSATDLTKL
                     GMLAMQNPVIADIVAKPSADLPVAGTVQNYNAVLGYDGVVGLKTGNNDGDLGAFLFAA
                     KQTIAGRQVTIVGTIMDAPSLYRALLDSLPLIRSVSADFKPVTVAAAGQTVGSIQVPW
                     HAPVNLQVKNSVQAVTWPGQQLTGHVELDTVRLPVSQHTVIGKLRFHDPLTKQDKSQN
                     IYAATALPKPGLLWRLQHLW"
     gene            2881..3453
                     /locus_tag="VFH39_05010"
     CDS             2881..3453
                     /locus_tag="VFH39_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET6925164.1"
                     /translation="MVVIIIVIAVIVLIGIILGAMYNGLVRLNVRADEAWSDITVQLK
                     RRADLIPNLVNTVKGYASHEKTVFEDVTAARAATVNAAGQGPAEAAKAENQFQQTLKS
                     LFAVAEAYPELKANQNFLDLQAQLTDTEDKIQASRRFYNGVVRDFNTKRKVFPTNIFA
                     NMLGFKQDKEFFEVDESQLAAIQNPVNVQF"
     gene            3506..4384
                     /gene="htpX"
                     /locus_tag="VFH39_05015"
     CDS             3506..4384
                     /gene="htpX"
                     /locus_tag="VFH39_05015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET6925165.1"
                     /translation="MYSQIASNKRKTVFVMLVFVAFVALIAWLFDQFLGGSPSIFYGV
                     LIGSVVYALITYFAGARMALAVNGAHEIQKTDNPRLWNIIENLAITDGLPMPRVYIMD
                     DPSPNAFATGRDPDHAAVCATTGILQIMNDTELQGVFAHELGHVKNYDIRVSMIAFAL
                     TAVVSLIADVILRMTWFRNDDRENNNQAVFVLAIIAAILAPLIASLIQLAVSRSREYL
                     ADATGALTTRYPEGLASALEKIEQTGSVLRKQNTATAHLFFANPLKGHSLANLFSTHP
                     PIEERIRRLREMETHA"
     gene            4415..>4626
                     /locus_tag="VFH39_05020"
     CDS             4415..>4626
                     /locus_tag="VFH39_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925166.1"
                     /translation="MEAITKTGTGTKTSGSNRRVKAPKRRFYKRQDKRFKARPKLINS
                     FRVSNRAALLIWRHRKVIGGVVLVYGL"
BASE COUNT          972 a         1305 c         1299 g         1050 t
ORIGIN      
        1 tccgcttata tagcggcctt tttatatggt ggtaaagagt gactaacgaa caggttcgta
       61 gaaccggcct tttcctggcc ggttctagag cgatgcaaag cgagcgaccc ctcatcaatc
      121 ctcaaaaaag aaccgggcaa cccggttctt ttttagtcaa tgtactactg catttagcga
      181 taagcgttca acaatttgct ttaccgtatc gatatcgtca ggctatcgac gtatatgtta
      241 tgcagagggc ttaggttcgt tgcgttcggt tggtcggtta catcgacttg tgagtagccc
      301 aggtagcttt tggtagccag tttgaagtca tagcaatgct tttcgtattt gttgccaagt
      361 agtatcgtct tctgcatcgt ggccgagcta gacgaagtca tattgacgag gtccaagctc
      421 agctttgaac tgtgctcggc ccgcgccacg acacagaccg ttgccggttg ggtggcatgg
      481 ttgttcagga aatcacgcaa tttcttagat gagttgtaac gtaccgccgc tgagccatcg
      541 cccctctcag tatctgcctt gagtaacagg acattcccgt ggctaccgtc tttgacgaca
      601 cctatactgt tcgtgtctat tttctgaaat ggtacccagt ccacgggccg caacaagatt
      661 gaagccgtgt cggcgtggct ggcgaccagg aagtacgagc cgaggatacc ggcgccgact
      721 atgaccagca gcaggaccag ggtatgggtc acgccggaag tgttaagttt acgaagtttc
      781 atgaggtcgc tcctgtttag gtatattgcc gttacgctta cacataagca taggcatttt
      841 tgtgttgtca aaagaaagtc ggcgttcggt tgttaaaaat gctatggttt gtaacttggc
      901 tcggttacgc ctgcatgttt taatagccat atcaactccc acacaaatac ccccctgcaa
      961 aacctgaaac tcgttctgcc gaccgtgttc gagtatgtcg gcttactgtt cgtggcgctc
     1021 atgatactgt tactcggcaa cacccagcag ctgttggagt attatgggtt cgacacctca
     1081 ggccaggtca tcaagcaggg cgcacatcag gcgatcgcct ccggtttatc caagctcgac
     1141 ggcttgagct ttaccgatcg ggtggtcacc ttcgtggtct gggcgcttat aggggtcgtc
     1201 tgcttcagtc tggtacagct gataggccgc gtttaccatg acttcgaaga ggaagaaaaa
     1261 gtgagctcca accgctatgt tcacccgagc accttccggc gcgccgattt ctggaaacag
     1321 gtggcgctcg acttcatagt agccacgata agcctggggc tgcttgtggc cggcgtggta
     1381 ctgctgttcg cctacgtgct tcctgtagcc ctcagttaca gcgagtcttt cctgacgcat
     1441 gcctcgctat cgcacttcat aaacttcttg gtcggattct ttatggtcta tgtgtggtca
     1501 ctggtgctgg ccgtaatctt taagtactac ttgcatcggc accggttgct ggcccgctga
     1561 atctaccaca gatgctgcag gcgccagagt aagcccggct tgggcagcgc cgtggcagcg
     1621 tagatatttt ggcttttgtc ctgtttggtg agcgggtcat ggaaccgcag cttgccgatg
     1681 acagtatgct gcgaaaccgg caggcggacc gtgtcaagtt cgacgtgacc agtcagctgc
     1741 tggccaggcc aggttaccgc ctgaacgctg tttttgacct gcagattgac cggtgcgtgc
     1801 cagggtacct gaatgctgcc taccgtctgg ccggcggcgg caaccgttac cggtttgaag
     1861 tcggcgctga cgctccggat aagaggaaga ctgtcgagca gcgcccggta caggctaggt
     1921 gcatccatga tagtaccgac gattgtcacc tgtcggccgg cgatcgtttg cttggcagcg
     1981 aacaggaaag caccgagatc gccgtcgtta ttgccggtct tgaggcctac gacgccatcg
     2041 tagccgagta cggcgttgta gttctggacg gtaccggcca cgggcaggtc ggcacttggt
     2101 ttggcgacga tgtcggcaat gaccgggttc tgcatggcca gcatgccgag ctttgtcaga
     2161 tcggttgccg acccggtagt gtcgctcgaa aagccgctgg cacccgcgaa gtgcaaactg
     2221 ttccaaccat gctccacggc gaactgattg gcatagttgg cgtaatcatc gagagagccg
     2281 aacgcccagc gcgccaaagt atcagccata ttgttgcctg aaggtagtag cattccctcc
     2341 agtgcctggt attcgctgat ctgctcgccg gcttgcaccg gcacgaccga gccatcctgg
     2401 gaaagatagg agttgtagcc gtccacgtcg gcttgggtca tggtaatcat cgggccggac
     2461 tggtggagcc cgagcggctt gcgctgcagc accgacaggg ccgtcatcac cttggcgata
     2521 ctggcggtgg gcgtaggtgt ttgatcgccg tgggtcgcca ggactccaaa gccgctgacg
     2581 gcgacagctg cctgaccgta agaaggccag ttgagcgccg gctcgccacg taattgcgcc
     2641 gtgacggggc gggtaatggt gaccggagcc ggcagaggtt tcgcggcggc gtaccctagc
     2701 gtggcaaccg ccaacagtac cactagtaat agggccacga atatctggtg ggcatgacgc
     2761 ttgtgcaccg gccggtgcgc gggaactggt cggacaggca ttacttttat gataagccgt
     2821 aaccgcgtat aatgacagcc acaaacaact cgtaaattac aacaggagtg tcccactgtc
     2881 atggtagtaa tcatcatcgt tatcgccgtc atcgtcctca tcggtattat tctgggcgcc
     2941 atgtacaacg gattggtccg cctgaatgtc cgggccgacg aagcctggag tgacatcacg
     3001 gtccagctga agcgccgggc cgaccttatc ccgaacctcg tcaacaccgt caagggctac
     3061 gcctcgcacg aaaagaccgt ttttgaggac gttactgccg cccgggctgc tactgtcaac
     3121 gccgccggtc agggccctgc cgaggctgcc aaggctgaga accagttcca gcagacgctc
     3181 aagagcctgt ttgctgtagc cgaagcttac ccggaactga aggccaacca gaacttcctc
     3241 gaccttcagg ctcagctgac cgacaccgaa gacaagattc aggcctcccg ccgcttctac
     3301 aacggtgtgg tccgcgactt caacactaag cgcaaagtct tcccgaccaa catcttcgcc
     3361 aacatgcttg gtttcaagca agacaaagag ttcttcgagg tcgacgagag tcagctggct
     3421 gccatccaga accccgtcaa cgtccagttc taagccgctg cctcagctga gtctcatata
     3481 gtttaaatag gatacaggct ctcccgtgta tagtcagata gcttccaaca aacgcaagac
     3541 cgtcttcgtg atgctggtct ttgtggcctt cgtggcgctc atcgcctggc tgtttgacca
     3601 attcctgggc ggaagtccga gtattttcta cggcgtgttg ataggttcgg tcgtatacgc
     3661 gctcattacc tacttcgcgg gcgcgcggat ggcactggcc gtgaacggcg cgcacgagat
     3721 tcaaaagact gacaacccgc ggctgtggaa catcatcgag aacctagcca ttaccgacgg
     3781 cttaccgatg ccgcgggtgt acattatgga cgatccgtcg cccaatgcct tcgccaccgg
     3841 ccgcgacccg gatcatgctg ccgtctgtgc caccaccggc attttgcaga tcatgaatga
     3901 caccgagctg caaggggtat tcgcacacga gctgggacat gtgaagaact acgacatccg
     3961 ggtgagcatg atcgcgttcg cgctgaccgc cgtggtgtcc ttgattgccg acgtcatctt
     4021 gcgtatgacc tggttccgca acgacgaccg cgagaacaat aaccaggcgg tgttcgtgtt
     4081 ggccatcatc gcggccattc tggcgccgct tatcgccagc ctcattcagc ttgcggtctc
     4141 acgcagtcgg gagtacctgg ccgacgctac cggtgccctc actacccgct accccgaagg
     4201 tctggccagc gccctcgaaa aaatcgagca gaccggcagc gtgttgcgca agcagaacac
     4261 tgccactgcg cacctgttct tcgccaatcc gctcaaaggt cacagcctgg ccaacctgtt
     4321 cagtacgcac ccgcccatcg aggagcgtat ccgccgcctg cgcgaaatgg agacccacgc
     4381 ctaactgccg tactctgcta caatacgtat aaatatggag gcgataacca aaaccggtac
     4441 cggcaccaaa acgtcggggt caaaccgccg tgtcaaggca ccgaagcggc ggttttacaa
     4501 gcggcaggac aagcgtttca aggcccggcc gaagcttatc aactcgttcc gggtcagtaa
     4561 ccgcgccgct ttgctgattt ggcggcaccg caaagtcatc ggcggtgtgg tattggtgta
     4621 cggcct
//
LOCUS       DASSFD010000207         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1132372, whole genome shotgun sequence.
ACCESSION   DASSFD010000207 DASSFD010000000
VERSION     DASSFD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132372"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFH39_05025"
     CDS             <1..1109
                     /locus_tag="VFH39_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HET6925167.1"
                     /translation="LTLTKGSVDQATGNTTYTLKATGSAKNATITGYTFSFGDQTSDK
                     TVKTSATSTSVTHSNAPGENTAKVTVTGKDNNGKPLTATSANCQKSVSVPKPNSNLTC
                     DELKLTPGAIGSDGSQAFVLTANATAKNATITGYTFSFGDQTSDQTVTTSATTATANH
                     TYAPGTYTAKVTVNGKDNSGKAITATSANCQQPVNVSPKQNPNITITKHVDGQKTEQV
                     DVNQQFVYQLVITNNGQQTLTNAVVTDPAPNGVTMLSADLGTVDTTKNSWSYTIPSLA
                     VGESVNVNITAVVKQYMAGNIVNTACVNAPEVNPEQPNQPDSCDTATVTVTPPAAPQQ
                     LVNTGAGSVATIFAAAAIVGTALHRYFLTRRLSE"
     gene            1368..3170
                     /locus_tag="VFH39_05030"
     CDS             1368..3170
                     /locus_tag="VFH39_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925168.1"
                     /translation="MLQRIGHRSHKVLLSAVLTCTLALGLAAWLQPVASANDCDNNAI
                     IHCGTGSTAEFIQKVKTNDSLNGFHDLKAVYAHYGLEPADYDRFVSSARPGTAYQDGR
                     IVVDGVTVATDAKSIGRLASYQGSGYFTTYINGHAYYGNIDSRAFKASSIPVMVLFDA
                     KGVMQFAVLTSCGNPVYGSPNKPSYSCNKLHSQAVAGQPGTYRFTTDASADTSAGAHI
                     AKLVYDFGDNSRQVTVTNPNQAVVHTYARSDSYTAKVTVYVNLPGKQTVTVTSSNCQT
                     VIKVSLPFYQCVQLAATALDNARYSYKFVTTTKAGNGARVQSVTFDYGDGHKDTIKQG
                     TNGKFASAHKYTKDGNVMAVATATVLLPGGKTVDVSGPNCRHKVPVAIPYYSCATFNG
                     SLLTDKPNHYKFTVTADYSKEVSFKKAVFDFGDKSQPRTVVPNGTAVTTDYTYASAAK
                     YEVTATLYFTIGKQSATDKCSVLVTPTQQVVQECLPGIPQGSSLCNCQYNAQLPANSD
                     QCVLPNTGAGSFVTLFGAAVVAGFFIFRQFIYKRLAPGGDVATTGPAPAAPSVPQVVA
                     PKVHRNAQQPLHHARYHQPHRFRPGSHHEHHTED"
     gene            3403..3477
                     /locus_tag="VFH39_05035"
     tRNA            3403..3477
                     /locus_tag="VFH39_05035"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3436..3438,aa:Glu,seq:ttc)
BASE COUNT          943 a         1307 c         1039 g          686 t
ORIGIN      
        1 acctgaccct gaccaagggc agcgtcgacc aggcgaccgg caacaccacc tacaccctga
       61 aggccaccgg cagtgccaag aacgccacta tcaccggcta caccttcagc ttcggtgacc
      121 agaccagcga caagaccgtc aagaccagtg ctaccagcac cagtgtcact cacagcaacg
      181 ctcctggcga aaacacggcc aaagttaccg tgaccggtaa ggacaacaac ggcaaacccc
      241 tgacggctac ctccgccaac tgccagaagt cagtcagcgt accgaagccg aacagcaacc
      301 tgacctgcga cgagctcaag ctcaccccgg gtgcgattgg cagcgacggc agccaggcct
      361 tcgtcctgac cgctaacgcg accgccaaga acgccaccat caccggctac accttcagct
      421 tcggtgacca gaccagcgac cagaccgtta cgaccagcgc tacgactgct actgccaacc
      481 acacttacgc acctggcacc tacaccgcca aggtcactgt gaacggcaag gacaacagtg
      541 gcaaagctat caccgctaca tcggctaact gccagcagcc ggtgaacgtc agccccaagc
      601 agaacccgaa catcaccatc accaagcatg tcgatggcca gaagaccgag caggtcgatg
      661 tgaaccagca gttcgtctac cagctggtga tcaccaacaa cggccagcag accctgacca
      721 acgccgtggt caccgacccg gccccgaacg gcgtcactat gctgagcgcc gacctcggta
      781 ccgtcgacac gaccaagaac agctggagct acaccatccc gagcctggct gtcggtgaaa
      841 gcgtcaacgt caacatcacc gccgtggtca agcagtacat ggccggcaac atcgtcaaca
      901 ccgcttgtgt gaacgccccc gaagtcaacc cggaacagcc gaaccagcct gactcctgcg
      961 acaccgcgac cgttacggtc acccccccgg ccgccccgca gcagctggtc aacaccggtg
     1021 ccggtagcgt cgccaccatc ttcgctgccg ccgccatcgt gggcaccgca ctgcaccgct
     1081 acttcctgac gcgccgcctg tccgagtaat cggtaccagc gaagcgctca gccgcgtcag
     1141 ccactggcca aatcggcaaa aatgtgctag actgccagca agtagcaaga gcaaccgtga
     1201 ctgaccgctc ctgaaacgaa aaatcaaacc tactgaaaac ccccgggcat gctagtccgg
     1261 gggttttctt tgcgccgcaa aacggagttt tattgacagc gtttcgctaa aagtgctacc
     1321 gtttaggcaa agcccgtaaa acaaaacacc agaaaaagga aatcgatatg cttcagcgca
     1381 tcggacaccg cagccacaag gtcctcctgt cggcggtgct tacctgtacc ctcgcccttg
     1441 ggctggccgc ctggctgcaa ccggtcgcca gcgctaacga ctgcgacaat aacgccatca
     1501 tccactgcgg gaccggctcg accgcggagt ttatccagaa ggtgaagact aacgacagcc
     1561 tgaacggctt ccatgacctg aaggccgtgt atgctcacta cggcctggag ccggccgatt
     1621 atgaccggtt cgtgagctcg gcccggcccg gtacggcgta ccaggacgga cggatcgtcg
     1681 tcgatggcgt gacggtggcg accgacgcca agagcatcgg ccggctggcg tcctaccagg
     1741 gcagcggcta cttcacgacg tacatcaatg gccacgctta ctatggaaac atagactccc
     1801 gggccttcaa ggcaagcagt atcccggtga tggtattgtt tgacgccaag ggtgtgatgc
     1861 agttcgcggt cctgacatca tgcggtaatc cggtttacgg ctcgcccaac aaaccttcct
     1921 acagctgcaa taagctgcac agccaggctg ttgccgggca accaggcacc taccgtttca
     1981 cgaccgatgc cagcgccgat acgagtgccg gggcgcacat cgccaaactc gtatatgact
     2041 tcggagataa cagccggcag gttacggtca cgaatccgaa ccaggcggtg gtccacacct
     2101 atgcgcggag cgactcctac accgccaagg tgacggtata tgtcaacctg cccggcaagc
     2161 agacggtaac tgtgacgtct tctaactgcc agacggtcat caaggtatcg ctgccgtttt
     2221 accaatgcgt ccagctggcc gcgacggcgc tcgacaatgc gcgctacagc tacaagttcg
     2281 taaccaccac caaggccggc aatggcgcca gagtgcagag tgtgacgttc gactacggcg
     2341 acggccataa ggacacgatc aagcagggaa cgaacggtaa gttcgcgtcc gcgcataagt
     2401 acaccaaaga cggcaatgtc atggcggtcg ccaccgcaac agtcctgctg ccgggcggca
     2461 agacggtcga tgtgagcgga ccgaactgcc gacacaaggt gcccgtggct ataccgtact
     2521 actcctgtgc taccttcaac ggatcgctgc tgaccgacaa accgaaccac tacaagttca
     2581 ccgtgacagc ggattactcc aaagaagtca gcttcaaaaa ggccgttttc gacttcggcg
     2641 acaaatcaca gccccgcacg gtagttccga acggtacggc ggtcaccacg gattacacgt
     2701 atgcctcggc cgccaagtat gaggtgacgg cgacgctgta cttcaccata ggcaagcagt
     2761 cggctaccga caagtgctca gtgctggtaa cgccgaccca acaggtagtc caggaatgcc
     2821 tgcctggcat cccgcagggt agctcgctgt gcaactgtca gtacaacgcg cagttgccgg
     2881 ctaactccga tcagtgtgtg ctgccgaaca ccggtgccgg ttcgttcgta acgttgttcg
     2941 gtgccgcggt ggtggccgga ttcttcatct tccgacagtt catttacaaa cgcctggcgc
     3001 cgggcggcga cgtcgcgacg accggccctg cacctgcggc gccgagcgtg cctcaagtcg
     3061 tcgcaccgaa ggtgcaccgc aatgcccagc agccgttgca ccacgcccgt taccatcaac
     3121 cgcaccgctt ccgcccgggc tctcatcacg agcaccatac cgaagactag tcacttccat
     3181 ttccgtatct gttatggtac gatattgcga gccgtttggc gtaccggatt agcgccgaag
     3241 catagtgcca atggcccctc acgcttcgcg tgcggtgcca aatccacggg acgagctatt
     3301 agctggcacg tttcggtact cggccccttc tgctggcgca gaacgaccca ttttttcgga
     3361 agatggaaat atgcaagcac atttccgcct tccttcaaaa atggcccctt cgtctatcgg
     3421 ttaggacatc gggttttcat cccggcaaga ggagttcgac tctcctaggg gtcaccacga
     3481 aaatagctca ccgcaaggtg ggcttttttc gtggtcgctg ttgaacttca aacaaccctg
     3541 aggccctatg ctatacttgc agcaaataag cacaagcagc tttgggaggg ctgaaaaaag
     3601 aaacaccatg gaaccgacaa acgacaagcc gacagatggc gaccctgctg ctttaccctc
     3661 aacccagaac caacacaccc ccaacgatcc tggagccact agcgagccag tggcttcggc
     3721 cgacgatgtc ccaagtgaac aaccagcagg cacagtgccg actactgaag cggagcctca
     3781 gacggcaccc ttagataccg tcccgcaaac tccttcttta gaaaatggta tttctgacga
     3841 gcagtcggct gccgggcagg tgtcgcctgt agtggagccc gctgacgctc cagccacaaa
     3901 gagctctggc cctacaggca ctggtccagt catactaccc cctcagttta cttcggtgtc
     3961 tgactctacc cctac
//
LOCUS       DASSFD010000208         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1259265, whole genome shotgun sequence.
ACCESSION   DASSFD010000208 DASSFD010000000
VERSION     DASSFD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259265"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="radA"
                     /locus_tag="VFH39_05040"
     CDS             <1..1074
                     /gene="radA"
                     /locus_tag="VFH39_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET6925169.1"
                     /translation="IAGQPGIGKSTLLLQLAYAAAANVPVLYVSGEESAHQVGMRAKR
                     LGTTRPELQIATSTSAEDIATTIMSGTYKLVIVDSIQTISVGSIASAAGTVSQITNST
                     HLLTMAAKQSNTAVVLVGHVTKEGSIAGPKVLEHLVDVVLQLEGDRYGGFKILRAIKN
                     RFGSTNEAGIFEMVEEGLKPVENPSAALLAERQISDGSVVLATLEGTRPLLVEVQALV
                     NKTSYGYPKRAASGMDLTRLNLLVAMLERRTKLNLADQDIYINIVGGIRVTEPAADLA
                     VCLAIGSAAKGMQLKQNAVVFGEVGLSGEVRHVPFAEKRIAEAKKLGFDCAIGPMQRG
                     TGKKLSFLTGVPDVRSALNQFLG"
     gene            complement(1160..>2553)
                     /locus_tag="VFH39_05045"
     CDS             complement(1160..>2553)
                     /locus_tag="VFH39_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925170.1"
                     /translation="LAASIGDGAFLHLDDHVNGLATLARMGQAIPRTFILQITDASNK
                     TIYKWTQPKSKQVIKPDTAYILNNMASDPNASYLGGHCTATNCTGYKFHRYNGWDFAI
                     KTGTTNNNYDGLMTSWSTKYAFVSWVGSQNRNKALSTEMEYLTEPITKGWMQYAHQDL
                     KPVNWTTPADIKTLPAFVIRSHIYAGDVVPSPTTDLFPSWYAGGAKGSSSAQTIDKVS
                     GKLATSCTPALAKTVQYNTNAASWNADIFMGGHNSVGSNSTGTTGITATDDVHSCSDS
                     PPSITLTLPPGNTCDTSCTVTATVSQGTHPLTDPAYPDYPGTVTFTLNGQTIHTAQVS
                     DSPSTISFTYAPSTSGSGTVTATVTDSVLYSATDSGTLNFTAPQPVAGQTLTLSLNGH
                     TSTAADFRWNGINGASSYRLCVTGPGVNNCQNFSGTSGTINAVYRPGQTYSATVTAND
                     SSNTTSDPVTWKA"
BASE COUNT          509 a          677 c          809 g          558 t
ORIGIN      
        1 atcgccggcc aaccgggtat cggcaaaagc acgctgctac tgcagctggc atatgctgcc
       61 gcagccaacg tgccggtgtt gtacgtgagc ggcgaagaat cggcgcatca ggtaggcatg
      121 cgggccaaac gtctcggtac gacccgcccg gaactgcaaa tcgccaccag cacctcggcc
      181 gaagacatcg ccactaccat catgagcggc acttataagc tggttatagt ggattccatc
      241 caaactataa gcgtcggttc catcgcttca gccgccggta cggtatcgca gatcaccaat
      301 tcgacccacc tgttgaccat ggcggccaaa cagagcaata ccgccgtcgt actggtcggt
      361 cacgtaacca aggagggctc gattgccggg cccaaagtgc tggagcatct ggtagacgtg
      421 gtgctgcagc tggaaggcga ccgctacggc ggcttcaaga tattgcgcgc catcaagaac
      481 cgcttcggct ccacgaatga ggctggcata ttcgagatgg tggaggaggg gctgaagccg
      541 gtcgagaacc catcggccgc gttgctcgcc gagcggcaga ttagcgacgg ttcggtggtg
      601 cttgctacgc tggaaggcac ccggccgctg ctggttgagg tgcaggcgct cgttaacaag
      661 accagctacg gctaccccaa acgcgccgcc agcggcatgg atcttacgcg gctgaatctg
      721 ctggttgcca tgctggagcg gcgtaccaaa cttaacctcg ccgatcagga tatatatatc
      781 aacattgtcg gcggtattcg cgtaaccgag ccggcggccg acctggccgt ttgtctggcc
      841 atcggtagtg ccgccaaagg catgcagctc aaacagaatg ccgtggtatt cggcgaagtg
      901 ggcttaagcg gcgaagtccg ccatgtaccg ttcgccgaaa agcgcatcgc cgaagccaaa
      961 aaactgggct ttgactgtgc tatcggcccc atgcagcgcg gcacgggcaa gaaacttagt
     1021 ttcttaaccg gcgtgccgga tgtccgcagc gctctgaacc aattcctcgg atgagagtta
     1081 tatagatgcc ctggcaccta caaaacagta cgccggatat ttctgttctc agccgctccc
     1141 cggtacggga cagttaagct caggccttcc acgttacagg atccgacgta gtgttgctgc
     1201 tatcatttgc cgtgacagta gcgctgtatg tctgaccggg gcgatatacg gcatttatgg
     1261 tgcctgatgt accgctaaag ttctggcaat tattaacacc gggaccggtt acacagagcc
     1321 tatagctgct cgccccgttt ataccgttcc atctgaaatc agccgccgtg cttgtgtggc
     1381 cgttgagact tagggttaag gtctgaccgg ccaccggttg gggcgccgta aaatttagcg
     1441 taccgctgtc ggttgccgag taaagaacgc tgtcggtcac cgtggccgtt accgtgccgc
     1501 tgcccgatgt agagggcgca taggtgaagc tgatagtgga cggcgaatcg gaaacctgcg
     1561 ccgtgtggat agtctggccg ttcagggtaa aggtgacggt gccggggtag tccgggtagg
     1621 ccggatcggt gagcgggtgc gtgccctgtg agactgtggc ggtaacggtg cagctggtat
     1681 cgcaggtatt gcccggcggc aaggtgaggg ttatggacgg cgggctgtcg ctacagctat
     1741 ggacgtcgtc ggtggcagtg ataccggtgg tgccggtact gttagaaccg acactgttat
     1801 ggccacccat gaagatatcg gcgttccagc tggcggcatt ggtgttgtac tggacggttt
     1861 tggcgagggc cggcgtgcag ctggtagcca gtttgccgga aaccttgtcg atggtctgcg
     1921 ccgaggagct gcccttggcg ccgccggcgt accaggaggg aaacagatcg gttgtcgggc
     1981 tgggtacaac gtcgccggcg taaatgtggc tgcgtatgac aaatgccggc agcgttttta
     2041 tgtcggcggg cgtggtccag ttgacgggtt tgagatcctg gtgggcatac tgcatccagc
     2101 ccttggtgat tggctcggtc aggtattcca tttcggtgct caaagcctta ttgcggttct
     2161 ggctgccgac ccaggatacg aaagcgtact tggtcgacca gctggtcatg agtccgtcgt
     2221 agttgttatt ggtggtgccg gtcttgatag cgaaatccca gccgttataa cgatgaaact
     2281 tgtaaccggt gcagttggtg gccgtacagt ggccccccag ataggacgcg ttcggatcgc
     2341 tcgccatgtt gttcaaaata tatgccgtat ccggtttgat cacttgctta gacttgggct
     2401 gcgtccattt gtaaatggtc ttattgctgg catcggtaat ctgcaggatg aaggtacgcg
     2461 gtatggcctg tcccatgcgg gccagagtgg cgaggccgtt gacgtggtcg tccaggtgca
     2521 ggaaggcacc atcgccgata gacgctgcca ggt
//
LOCUS       DASSFD010000209         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1239929, whole genome shotgun sequence.
ACCESSION   DASSFD010000209 DASSFD010000000
VERSION     DASSFD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239929"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..610)
                     /locus_tag="VFH39_05050"
     CDS             complement(128..610)
                     /locus_tag="VFH39_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925171.1"
                     /translation="MSENSEHLRDVYDWRPYYIYTPEAGTVSEFSVIDPPLPVTEDQM
                     GILAGLPLVVHEFHTHKNLQEDMPANEQPVAEVRIADIVKALRAASGEKIRQFWAAQW
                     DAYLKNVISSNPSYRGKGIARALGPTLDLLVEHGELHEVVPDQAVDEYWLPPDAQQAS
                     "
     gene            complement(626..1657)
                     /gene="recA"
                     /locus_tag="VFH39_05055"
     CDS             complement(626..1657)
                     /gene="recA"
                     /locus_tag="VFH39_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET6925172.1"
                     /translation="MAKSTKVTTDEATATGTSEDGKSKALGLALETIEKQFGKGSIMK
                     LGEAHATKVECIPTGSISLDLALGGGIPKGRVIEIYGPESSGKTTLTLHAIAEVQRQG
                     GTAAFIDAEHALDPAYAQRIGVDVQNLLLSQPDNGEQALEIVETLVRSNAVDLIIVDS
                     VAALVPRAEIEGDMGDAQMGLQARLMSQALRKLTAVISRSKATVIFINQLRMKIGVMF
                     GNPETTTGGNALKFYASVRMDIRRIGQIKQGDSVIGNRTRVKVVKNKIAPPFREAEFD
                     IMYNQGISASGDILDLAVAKNVIEKAGAWFAYDGEKLGQGREAAKSYLQENPKVMAEV
                     AEKTRAAVE"
     gene            1846..2373
                     /locus_tag="VFH39_05060"
     CDS             1846..2373
                     /locus_tag="VFH39_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET6925173.1"
                     /translation="MAGRINKYSLWLVPEGRAGEALQALVSAVAAETDTPPFVPHLTL
                     VAHLSASAQTLPELKQRIATLAGLLHPIQLEFIDFGSLDEKSRSLFIEVRREPLRAVY
                     QTAATVFPVVRDEHFAALPHISVLYGLHPEGVKRRLIQGHALPVGTAAVSALSLFLTE
                     GTPSTWRLEQSFPLS"
BASE COUNT          439 a          698 c          673 g          583 t
ORIGIN      
        1 accggtaatc atatggttcg taacggtcat gggtacgttt tcgtttatag gcctgaagat
       61 tcagccgatc actcacagtg tagcggattg gaggctgaag gggcaaaacc ggtctttggg
      121 atattggtta ggatgcttgc tgcgcgtccg ggggaagcca gtattcatcg actgcctggt
      181 ccggcactac ttcgtgcaac tccccatgtt caaccagcag atcgagcgtc gggcccaatg
      241 cccgggcaat gcctttgccc cggtacgagg gattgctgga gatgacattc ttaaggtagg
      301 cgtcccactg ggcggcccag aactgccgta ttttttcgcc ggaggcggcc cgcaacgcct
      361 tcacgatatc cgctatccgc acttcggcca ctggttgctc attggccggc atgtcctcct
      421 ggaggttctt atgtgtgtgg aactcgtgca ctaccagggg caggccggcc aatatgccca
      481 tctgatcctc ggtcaccggc agcggcgggt caatgacgct gaattcactg accgtacctg
      541 cctccggcgt atagatatag tatggccgcc aatcgtacac gtcccttaag tgttcactgt
      601 tttcgctcat aggcaaactt gcctcctact cgacggcggc gcgggtcttt tcggcgacct
      661 cggccattac tttcgggttt tcctgtaggt agctcttggc tgcctcacga ccctggccga
      721 gcttctcacc gtcataggcg aaccaggcgc cggccttttc tatgacattc ttggcaaccg
      781 cgaggtccag gatatcgccg gaggcgctga ttccttggtt gtacatgatg tcgaactcgg
      841 cttcgcggaa aggcggcgcg atcttgttct tcacgacctt cacgcgggta cggttaccga
      901 tgacgctgtc gccctgtttg atctggccga tgcggcggat gtccatgcgg accgaggcat
      961 agaatttcag cgcgttacca ccggtggtgg tttccgggtt gccgaacatg acaccgatct
     1021 tcatacgcag ctggttgatg aagatgacag tcgccttgct gcgcgaaatg acggcggtga
     1081 gcttgcgcag cgcctggctc atcagccggg cctgcaggcc catctgggcg tcgcccatgt
     1141 cgccttcgat ttcggcgcgc gggaccaagg ctgccacgga gtcgacgatg atcaggtcga
     1201 cggcgttact gcgcaccaag gtctcgacga tttccagagc ctgctcgccg ttgtccggct
     1261 ggctcagcag caggttctgc acgtccacgc cgatacgctg ggcgtaggcc gggtcaaggg
     1321 cgtgctcggc gtcgatgaag gctgccgtgc cgccttgccg ctgcacctct gcgatggcgt
     1381 gcagtgtcag ggtcgtctta ccggagcttt ccggtccgta gatttcaatg acgcggcctt
     1441 tcgggatgcc gccacccagg gctaagtcca atgagattga gccggtcgga atgcactcca
     1501 ccttggtagc gtgggcttcg cctagcttca taatggagcc tttaccgaac tgtttttcga
     1561 tggtctcgag ggcgaggccg agtgctttgc tcttgccgtc ttcagaagtg ccggttgcgg
     1621 ttgcttcgtc agttgtcact ttagtagatt tagccatgtt atatgtcctc ctttggcttc
     1681 gtcagtagta ttattgcttc gctttgtgtc atttaaccgg atggataaat aaccttttgg
     1741 cactttttat tgtactggaa atttatagca ctgtcactat gcttatgcca aacaaaaaac
     1801 taaaaattac aacgtcacca tctgtcaaat tgctatactt tcgtcatggc cgggcgcatc
     1861 aacaaatatt cattgtggct ggtgccggaa ggacgggccg gcgaagccct gcaagcgctc
     1921 gtttccgcgg ttgctgcgga gaccgatact cccccgtttg tgcctcattt gacactggtg
     1981 gctcacctat cggcctcagc gcagacgctc cccgagctga aacaacgcat agcaaccctg
     2041 gccgggctcc tccatccgat ccagctggaa tttatcgatt tcggcagcct ggacgagaag
     2101 agccgctcct tattcataga ggtgcgacgc gaaccattgc gagccgtata ccagactgct
     2161 gcaaccgtgt tccctgtggt tcgcgacgag cactttgcag ccctgcctca catcagcgtc
     2221 ttatacggcc tgcaccccga gggggtcaaa cgacgcctga tccagggcca tgccctcccg
     2281 gtcggcaccg cagcagtctc ggcgctcagt ctcttcctga cagaaggaac cccatccacc
     2341 tggcgcctgg aacaatcgtt ccccctcagc taagcgctta cggtttttgg gta
//
LOCUS       DASSFD010000210         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_740011, whole genome shotgun sequence.
ACCESSION   DASSFD010000210 DASSFD010000000
VERSION     DASSFD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740011"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..286
                     /locus_tag="VFH39_05065"
     tRNA            213..286
                     /locus_tag="VFH39_05065"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Cys,seq:gca)
     gene            316..401
                     /locus_tag="VFH39_05070"
     tRNA            316..401
                     /locus_tag="VFH39_05070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Leu,seq:taa)
     gene            582..1085
                     /locus_tag="VFH39_05075"
                     /pseudo
     CDS             582..1085
                     /locus_tag="VFH39_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
     gene            1110..1988
                     /locus_tag="VFH39_05080"
     CDS             1110..1988
                     /locus_tag="VFH39_05080"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET6925174.1"
                     /translation="MYSQIAANKRKTWFIMVVFIAVVTGLGWLFAAATGRPAITPYVL
                     IGAGFYVLISYLLGSSMALAVNGAQEITKKDDPRLWRTVENLAITDGLPMPRVFIIND
                     PAPNAFATGRNPNNSAVCVTTGLLDIMNHSELEGVIAHELGHVKNYDIRVSLIAFALV
                     AVVGVLADIMLRLVWFRDDEEEGNNNQIFLILGIVAAILAPLVATLIQLAISRQREYL
                     ADATGALTTRYPDGLASALEKIGQTGSTLKRQNSSTAHLFFANPLKSGGLANLFSTHP
                     PIADRVARLRKMSAGN"
     gene            1993..>2248
                     /locus_tag="VFH39_05085"
     CDS             1993..>2248
                     /locus_tag="VFH39_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925175.1"
                     /translation="MAEPKTKSTTGAGNRSVKPKAADAPRRVKATRLDRWRHRKTLRP
                     TVKKVPSAFKLTGQARRLLFKRWRLFGGIILVYLLLTLILV"
BASE COUNT          521 a          607 c          608 g          512 t
ORIGIN      
        1 ggtggagcgt ggtactggcg tcagccgttg ctacgcttcc gccaacaaag atgccgagac
       61 aggcgagacc gacgagaatc cggtgaggga gcttcattga agcgttcctt tcagggaatg
      121 attgtcctca atgaccacat aacattagca taataaataa aaattgtcaa tgtatgggca
      181 tagttgttgt ctctgttata atcggtggag ttggcgcctt ggcggagtgg ttacgcagag
      241 gtctgcaaaa ccttttacac cggttcaatt ccggtaggcg cctccagtac atgtttttgc
      301 cggtcggcat tagccgggcg agtggcggaa ttggtatacg cggcggactt aaaatccgct
      361 ggccaaaagc cttgagggtt cgagtccctc ctcgcccacc aacctaaaag cctcgtcgtt
      421 tgtcgaaggc tttttgtttg gcgttaattg ctgttctttc gccactgcaa atcggtttga
      481 tgctatactg aaagtatcaa tcataacagg agtagaacgc ttctatgaca ctcattatta
      541 ttctcgtaat cgttgcaatc gtgatcatcg ccggcattgc aatgtacaac agtctggtcc
      601 ggtccaatgt ccgtgtcgat gaagcgtgga gcgacatcac cgttcagctc aaacggcgtt
      661 atgacctgat tccgaacctc gttaataccg tcaaaggcta cgccacgcat gaacgcgagg
      721 tgtttgagaa cgtcactgcc gcccgcgcca atgcgctgaa cgcacaggga gtagccgaga
      781 ctgcgcaggc tgagaaccag ttccagcaga cgctgaaaag cctgtttgcc gtcgccgaga
      841 actatccgca gctgaaggct aatgaaaact tcaaggccct gcaggatgag ctggttgata
      901 ccgaagataa gatccaggcc tcacgccgtt tctacaatgg atcggctcgt gacctgaaca
      961 tcaagatcta gtcatttccg accaacattt ttgccggcat gctcggtttt cacaagcgcg
     1021 agttctttga actgactgaa gcagaacagg ctactgtcag ccaaccggtc gacgtcaagt
     1081 tctagtactg ctaatccgaa aggtcgttca tgtacagcca aattgcagct aataagcgca
     1141 agacgtggtt cattatggtg gtgtttattg ccgtggtaac cggtctcggt tggctgtttg
     1201 ctgccgctac cgggcgaccg gcgatcacgc cgtatgtcct gataggcgcc ggattctacg
     1261 tgctgatcag ttatctactc ggctcgtcca tggccttagc ggtaaacggc gctcaggaaa
     1321 ttaccaaaaa ggacgatcca cggctgtggc gtacggttga gaacctggcg attaccgacg
     1381 gattgcccat gccgcgggta ttcatcatca atgatcctgc accgaacgca ttcgctaccg
     1441 gtcgcaatcc caacaactcg gctgtctgcg tcacgaccgg gctgcttgat atcatgaacc
     1501 acagcgagct ggaaggcgtc atcgcccacg aactaggcca tgtcaaaaac tacgacatcc
     1561 gggtgtcact gatcgcgttc gccctggtgg cggtggtcgg tgtcctggct gacatcatgt
     1621 tgcggctggt ctggttccgg gatgacgagg aagagggtaa taacaatcag atatttctga
     1681 ttctcggcat cgtggcagcg attctggcac cgctggttgc gaccctgatc cagctggcca
     1741 tttcccggca acgtgagtat ctggccgatg caaccggcgc cctgaccacc cgttatcccg
     1801 atggcctggc cagtgcgctt gaaaaaatcg gccaaaccgg cagtactctc aagcggcaga
     1861 acagctcaac cgcccatttg ttctttgcga acccacttaa gagcggtggc ctggccaatc
     1921 tgttcagtac gcatccaccg attgctgatc gggtcgcccg gttacgaaaa atgagcgcag
     1981 gaaactagtc cgatggcaga accgaagaca aaatctacaa ctggagccgg taaccgctcg
     2041 gtaaagccaa aggcagccga cgcgccgcgg cgggtgaaag cgacccggct ggaccgctgg
     2101 cggcaccgta aaacgctgcg gccgacagtc aaaaaggtac cctcggcctt caagcttacc
     2161 ggccaggcca ggcggctgtt attcaaacgc tggcgcctgt tcggcggtat catcctggtc
     2221 tatttactac tgacgctcat actggtca
//
LOCUS       DASSFD010000211         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1369725, whole genome shotgun sequence.
ACCESSION   DASSFD010000211 DASSFD010000000
VERSION     DASSFD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369725"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..278
                     /gene="rpsN"
                     /locus_tag="VFH39_05090"
     CDS             9..278
                     /gene="rpsN"
                     /locus_tag="VFH39_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET6925176.1"
                     /translation="MAKKSIVARDLKRQKMIQKYAAKRAQLKELGDQEGLAKLPRNSS
                     PTRWKNRDFETGRPHGYMRQFGLSRISFREHASKGEIPGVTKASW"
     gene            289..687
                     /gene="rpsH"
                     /locus_tag="VFH39_05095"
     CDS             289..687
                     /gene="rpsH"
                     /locus_tag="VFH39_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET6925177.1"
                     /translation="MIASTDPIADMLSRIRNAIAVRKNTVTLPHSKVKESVAQLLKQS
                     NFITDVKVGEDKIGKLMTITINDEHENARITEIVRLSKPGRRFYASAQDIPTVKRGRG
                     VVIVSTSRGMMTGDEAKKQRVGGELICKVY"
     gene            773..1312
                     /gene="rplF"
                     /locus_tag="VFH39_05100"
     CDS             773..1312
                     /gene="rplF"
                     /locus_tag="VFH39_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET6925178.1"
                     /translation="MSRIGKLPIAVPSGVTITVDPDKIVVAGGKGTLEQFTMPGVTVK
                     QDGDQVLVTRANDEAPNRAKHGLMRSLVNNMVVGVSQGFSKKLEITGVGYRVALQGSD
                     LKFNLGLSHDVIYKMPAGVTATVEQNTITVSGISKQQVGQVAAEIRALKKPEPYKGKG
                     IKYEGERILRKSGKSGKDK"
     gene            1315..1671
                     /gene="rplR"
                     /locus_tag="VFH39_05105"
     CDS             1315..1671
                     /gene="rplR"
                     /locus_tag="VFH39_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET6925179.1"
                     /translation="MAKQTLKARNFTQRAARVRATVSGTTARPRLSVHVSNLHVSAQI
                     INDETHQTLAAVSTVGQKSVKGSLTEKATWVGTEIAGKAKAAKVKQVVFDRRGKLYHG
                     RVKALADAARNAGLEF"
     gene            1674..2297
                     /gene="rpsE"
                     /locus_tag="VFH39_05110"
     CDS             1674..2297
                     /gene="rpsE"
                     /locus_tag="VFH39_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET6925180.1"
                     /translation="MAVGQTQAPTRGQERRNRRPEVQQEEKQFDERTLHIDRVARVVK
                     GGRRFRFRALVVVGDRKNRVGIGLAKGADVTAAVNKATEVAKKHMVTVPLYKDTLPHE
                     VETKVAGARILIKPAAAGTGLIAGGVVRTILEVAGVKNALSKSLGSSNKTNTAYATIQ
                     ALQSVVPAKQWVTAKAVSSKRVAETKEATKAAAKRPAAKKTAESEEK"
     gene            2297..2770
                     /gene="rplO"
                     /locus_tag="VFH39_05115"
     CDS             2297..2770
                     /gene="rplO"
                     /locus_tag="VFH39_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET6925181.1"
                     /translation="MKYHELNISSHKTAKRVGRGISAGQGKTAGRGTKGQGSRTGSTR
                     RPGFAGGQNPLMQQLPKLPGFNSKRVKAENVFTDQLERLGKGSVDNVVLAEAGLVSSP
                     YVRVKLLKRGDLSKKVTVKLQGASETAVAVVQEAGGSFEAVARPQRPKTSVKEKK"
     gene            2864..3613
                     /locus_tag="VFH39_05120"
     CDS             2864..3613
                     /locus_tag="VFH39_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925182.1"
                     /translation="MGFTIFEEIYFYLYEYAVWIGAVSLVIGAVLYLILRAKSSPHKA
                     FSVVMPLILIIGGAYLILWNFRIINLPYRYPDTSSADAAQAIQENQNNARKITFKTFL
                     PAYTPTYVSSRTRILSVFINGDSQPQFTVEYHKYLSGLYINSDVFSLAETAPAGVITK
                     ETSRSGSCDSVEPLSDFYAVGKNADGGVIYKASGDGEYYLTDYQGTRLELHHCALDAT
                     DTTLQDADALKMLKSLTAFNVKTADFTDPRE"
BASE COUNT          966 a         1086 c         1064 g          739 t
ORIGIN      
        1 taagtaatat ggctaagaaa tctatcgttg cccgcgacct gaagcggcag aagatgatcc
       61 agaaatacgc cgccaagcgc gcccagctca aagagcttgg tgaccaggaa ggcctggcca
      121 agctgccccg taactccagc ccaacccgct ggaagaaccg tgacttcgag accggccgtc
      181 cgcacggcta catgcgccag ttcggccttt cccgtatcag tttccgcgaa cacgccagca
      241 aaggcgagat cccgggtgta acgaaagcca gttggtagga ggaaagttat gattgcatct
      301 acagacccca tcgccgacat gctgtcccgc atccgcaacg ccatcgcggt ccgcaaaaac
      361 accgtcaccc tgccgcacag caaggtgaag gaatccgtcg ctcagctgct gaagcagagc
      421 aacttcatta ccgacgtcaa ggtcggtgaa gacaagatcg gcaagctcat gaccatcacc
      481 atcaacgacg agcacgaaaa cgcccgtatt accgagatcg tccgtctgag caaaccgggc
      541 cgccgctttt acgccagcgc ccaggatatt ccgactgtta agcgtggccg tggcgtcgtc
      601 atcgtcagca ccagccgggg catgatgacc ggcgacgaag ccaagaagca gcgcgtcggt
      661 ggcgagttaa tttgcaaggt atattaatag aattcggata gtagattatg gattttagaa
      721 tgaaccacat tccagcttct ataatcaata atccatattc ggaaggaatc atatgagtcg
      781 tataggaaaa ctaccgatcg cagtgccgag cggtgtgaca atcactgtcg accccgacaa
      841 gatcgtggtc gctggtggca agggtacgct ggagcaattc accatgcccg gcgtgaccgt
      901 caaacaagac ggtgaccagg tcctcgtgac ccgtgctaat gacgaagcgc cgaaccgtgc
      961 caagcacggg ctgatgcgct cgctggtcaa caacatggtc gtcggcgtca gccagggttt
     1021 ctccaagaag ctcgaaatca ccggtgtcgg ttaccgcgtc gctctgcaag gcagtgactt
     1081 gaagttcaat ctcgggttga gccacgatgt catctacaag atgccggccg gcgtgaccgc
     1141 taccgtcgag cagaacacca tcaccgtcag cggtatcagc aaacagcagg tcggccaggt
     1201 ggctgccgaa atccgcgcgc tgaagaagcc ggagccgtat aagggcaagg gtatcaagta
     1261 cgaaggcgag cgcatcctgc gcaagtccgg taagtcagga aaggataagt agtcatggct
     1321 aagcaaacac tcaaggcgcg taactttacg cagcgtgctg cccgtgtccg ggctaccgtt
     1381 tccggtacga ccgcgcgccc acgtctgagc gtgcatgtga gcaacctgca cgtcagcgcc
     1441 cagatcatca acgacgagac gcaccagacg ctggctgccg tttcgaccgt cggccagaag
     1501 tccgtcaagg gcagcctgac cgaaaaagct acctgggtag gcaccgagat tgccggcaaa
     1561 gccaaggcag ccaaagttaa gcaagtggtg ttcgaccgcc gtggcaaact gtaccacggc
     1621 cgcgtcaagg ctctggccga cgccgcccgt aacgcaggat tggagtttta atcatggcag
     1681 ttggacaaac ccaggcaccg acccgtggcc aggagcgacg taaccgccgc cccgaggtac
     1741 agcaagaaga gaagcagttc gacgagcgca ccctgcatat cgatcgcgtg gcccgtgtgg
     1801 tcaagggcgg ccgccgcttc cggttccgcg ctctcgtagt cgtcggtgac cgcaagaacc
     1861 gcgtcggtat cggcctcgcc aaaggcgcgg acgttactgc cgccgtcaac aaagccaccg
     1921 aggtcgccaa gaagcacatg gtgaccgtac cgttgtacaa agacacctta ccgcacgaag
     1981 tcgagaccaa agtcgccggt gcgcgcatcc tcatcaagcc ggctgccgcc ggtaccggtc
     2041 ttatcgctgg tggtgtcgtc cggaccattc tagaagtcgc cggtgtgaag aacgctctga
     2101 gcaagtcgct cggctcgtct aacaagacca acaccgccta cgctaccatc caggcactgc
     2161 agagtgtcgt gccggccaag cagtgggtta cagccaaggc tgtcagcagc aagcgcgtcg
     2221 ccgagaccaa agaagctacc aaagccgctg ctaagcgccc ggccgccaag aagactgccg
     2281 aaagcgagga gaagtaatga aatatcacga actgaacatc agcagccaca agaccgctaa
     2341 gcgcgtcggc cgtggtatct ctgccggtca gggtaagacc gccggtcgtg gtaccaaagg
     2401 gcagggctcg cgcacaggtt ctacacgccg tccgggtttc gccggcggcc agaacccgct
     2461 catgcagcag ctgccgaaac ttccgggctt caacagcaag cgtgtgaagg ccgagaacgt
     2521 cttcacggat caactggaac gcctgggcaa aggcagtgtc gataacgtcg tgctggctga
     2581 agccggcctg gtcagtagcc cgtacgtccg cgtcaaactg ctgaagcgcg gcgacctgag
     2641 caagaaggtc accgtcaagc tgcaaggcgc cagcgaaact gctgtggcag tcgtgcagga
     2701 agctgggggc agcttcgaag ccgtcgcccg cccgcagcgc cccaagacca gcgttaaaga
     2761 gaagaagtag aaggtacctt gtgcttcaga aaaggcctca aacgaggcct tttctagtag
     2821 gtgacaatag ctcaccgctg cgcttacatg tattataagc acaatgggtt ttacgatatt
     2881 cgaggagatt tacttctacc tgtacgagta cgccgtatgg atcggtgctg taagtttggt
     2941 tataggagcg gtcttatacc ttattttgcg agcaaaatcc tctccgcata aggcattctc
     3001 cgtcgtaatg ccgctgattt taattattgg cggagcctat ctgatactgt ggaatttcag
     3061 gattatcaac ctaccgtacc gctaccccga cacctcatcg gccgacgccg ctcaagcaat
     3121 tcaggaaaac caaaacaatg cgcgcaaaat tacctttaag accttcttgc ccgcttatac
     3181 tccgacctat gtgtcgtcgc ggacgcgaat actctcggtt tttatcaatg gtgatagcca
     3241 gccccaattc actgtggaat atcataaata cttatcaggc ctatacatca atagcgatgt
     3301 tttcagccta gccgaaacgg cgccggcagg agtaataact aaagaaacat cgcgttccgg
     3361 atcctgtgat tcggtggaac ctttatctga tttttatgct gtgggcaaga atgccgacgg
     3421 cggtgttatt tataaggcga gtggagacgg tgaatactat ctcactgatt accagggaac
     3481 acgtttagag ctgcaccatt gcgccctcga tgcaaccgat acaacgctgc aggacgccga
     3541 tgccttgaag atgctgaaga gtcttacggc gttcaacgta aagaccgccg actttaccga
     3601 tccacgcgaa taacgacgag cgcacggtac atatatgggc tcagaagctt tgcgggtata
     3661 gcacaaacct tgtatactct gtagagattc tagagactaa ctaaaagggt aacaaatctt
     3721 gaactggaag attatttgga agagtttgag ccataaggac atgcgcaagc gtatcttggc
     3781 ggtcctgggt atattgctgg tgttccgcat tttggcgcag attccggtgc cgatcagtaa
     3841 cggcgacaca ctccg
//
LOCUS       DASSFD010000212         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1127282, whole genome shotgun sequence.
ACCESSION   DASSFD010000212 DASSFD010000000
VERSION     DASSFD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127282"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VFH39_05125"
     CDS             complement(<1..1259)
                     /locus_tag="VFH39_05125"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6925183.1"
                     /translation="MDIPPAVKTKLDELPKQPGVYFHKDAKGEIIYVGKAARLNTRVR
                     QYFQSSRSRDPKTEALVREIADIDWMVVDSELEALFLEAEMIRRYMPRYNILLRDDKS
                     FAYIRIDYDSDYPTVSTTRRPLDDGARYYGPYSSTTSIRQALKLLRRVFPFATRRIPG
                     QKRATLHYHLGLDPGLEEGRTSLEDYRANLRRLIAVIEGKRRSIENDIEKEMRRAAKG
                     RDFERAARLRNQLLNLQSISQQVIFSDKEFLDISKDHALNELVNLLSLAEFPRRIEGY
                     DISHQQGTDVVASMVVFTNGVSNKAEYRKFKTKINHNNDFYNMHETLRRRLSEKNVKA
                     WGRPDLVLIDGGKGQLDAAIAARDEQAALQGAALQRVPFIGLAKREEQVVIHRAKSNV
                     TLNVEELHRLGGFVTESEDFLLVNVPH"
     gene            complement(1289..>2164)
                     /locus_tag="VFH39_05130"
     CDS             complement(1289..>2164)
                     /locus_tag="VFH39_05130"
                     /inference="COORDINATES: protein motif:HMM:NF001134.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET6925184.1"
                     /translation="ILLYYKFIPLADPEAVRLWQRALCASLNLKGRILLSPHGINGTL
                     GGDTADLKAYAKETKAYFTDMEFKWSDGGREHFPKLSVKVRPEIVTFGAAGELQVDGN
                     GVVGGGKRLTPAGVHELVQSRGEDVVFFDGRNAYEAEVGRFKGAVVPRVAHTRDFMNE
                     LEDPKYEALKDKPVVTYCTGGIRCEVLSALMKNRGFKEVYQLDGGIATYGEAYGDEGL
                     WEGGLYVFDGRLTVKFTDKAKDIADCAGCGVKTSDFVNCANVRCNRLLVMCRSCAATR
                     PYCGQCRPQAAPLER"
BASE COUNT          414 a          646 c          599 g          505 t
ORIGIN      
        1 ttgtgtggca cattaaccag taaaaaatct tcgctttcgg ttacaaaacc acctaagcgg
       61 tgcagctcct cgacattcag ggtgacatta cttttggccc tgtggatcac cacctgctct
      121 tcccgtttgg cgagaccgat aaaaggaacc ctctgcaagg ccgcaccctg caaagcagcc
      181 tgttcgtccc gggcggcaat cgccgcatcc agctggccct tgccgccgtc tatgagcacc
      241 aggtccggtc taccccacgc tttcacattt ttctcgctta ggcgccgtcg gagcgtctcg
      301 tgcatattat aaaagtcgtt gttgtggttg attttggtct taaacttgcg gtattcagcc
      361 ttgttactga cgccgttcgt gaagacgacc atgctggcca ccacgtcggt accttgctgg
      421 tgcgaaatat cataaccctc aatccggcgc ggaaattcgg ccaaacttaa caagttaacc
      481 aattcattca aagcatggtc tttactgatg tccagaaact ctttatcgct gaaaataact
      541 tgttggctga tactttgcaa gttaagtaat tggttgcgta gccgtgccgc ccgttcgaag
      601 tcccggccct tggcggcccg gcgcatctcc ttttctatgt cgttctcgat gcttcgccgc
      661 ttgccctcta taaccgctat gagccggcgc agattagcgc ggtagtcctc caggctggtt
      721 ctgccctctt ccaggcccgg atccagaccg aggtgataat gcaacgttgc ccgcttctgg
      781 ccgggaatcc ggcgggtcgc gaacgggaac acgcggcgca acagcttaag agcctggcgg
      841 atggaggtcg tagaagagta cggaccgtag tagcgggcac cgtcatccag cggccggcgg
      901 gtcgtactga cggtagggta gtcactgtcg tagtctatgc ggatgtaggc aaacgacttg
      961 tcgtcgcgca gcaggatgtt gtagcgcggc atgtagcggc ggatcatttc cgcctcgagg
     1021 aacagcgctt caagctcgct gtccacgacc atccagtcga tatccgcgat ttcgcgtacc
     1081 agcgcttcgg ttttggggtc gcgtgaccgg ctgctttgga aatactgccg cactctggta
     1141 ttcagacgcg ccgctttgcc gacatagata atctcgcctt tggcgtcttt atgaaagtaa
     1201 acgccaggct gcttcggcag ctcgtccagc ttggttttga cggccggggg tatatccacg
     1261 ctattatttt agcaactcag cccagcggtt accgctctag aggagcagcc tgcgggcgac
     1321 actgcccgca gtacggacgc gttgccgcgc agctgcggca catgaccagg agtcgattgc
     1381 accggacatt ggcgcagttg acgaaatcac tggtcttgac gccgcagccg gcacagtcgg
     1441 cgatatcttt ggctttatct gtaaatttca ccgtcaggcg cccgtcgaaa acatacaggc
     1501 cgccctccca gagcccctcg tcgccgtatg cttcgccata ggtggcgatg ccgccgtcca
     1561 gctggtatac ctctttgaag ccgcggtttt tcatgagagc gctcaggact tcacagcgga
     1621 tgccgccggt gcagtaggta acgaccggct tgtccttgag ggcctcgtac ttcgggtctt
     1681 ccagttcgtt catgaagtcg cgggtatggg caacgcgcgg cacgacggca cccttaaaac
     1741 ggccgacttc ggcttcgtag gcattacggc cgtcgaagaa cacgacgtct tcgcctcggc
     1801 tttggacaag ctcatgcacc ccggcgggcg tcagacgctt gccgcccccg accacgccat
     1861 tcccgtccac ttgcagctca ccggccgcgc cgaacgtgac gatttcgggc cggaccttta
     1921 cggacagttt cggaaaatgc tcccggccgc catcgctcca tttgaactcc atgtccgtga
     1981 agtaggcttt cgtctctttg gcatatgctt tcaggtccgc cgtgtcgccg ccgagcgtcc
     2041 cgttgatgcc gtgcggcgac agcaggatgc gtcccttgag gttcaggctg gcgcataagg
     2101 cgcgctgcca tagccgcacc gcttccggat cagccagcgg tatgaatttg taataaagaa
     2161 gtat
//
LOCUS       DASSFD010000213         8939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_814730, whole genome shotgun sequence.
ACCESSION   DASSFD010000213 DASSFD010000000
VERSION     DASSFD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814730"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..622
                     /locus_tag="VFH39_05135"
     CDS             131..622
                     /locus_tag="VFH39_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925185.1"
                     /translation="MSKETVSELQGRQASEQELYMYECVIEGLHRSIMAGHRFTGYDT
                     EVIGNMATLRMHDQPHRSLQISREAATGVWSIITRYRFEEGGDTSDGTDTWVTELRPI
                     AQLAGLSIVERCVEVPRMARHPGRVLASATKALPSWSAVQCWTGTREARELLALGTHM
                     QAS"
     gene            complement(641..1240)
                     /locus_tag="VFH39_05140"
     CDS             complement(641..1240)
                     /locus_tag="VFH39_05140"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET6925186.1"
                     /translation="MGKATAKRKTTIWQQLKAVNIRDDEHLLFPTFIIVLLTIIVINT
                     TYILNKQSAAAGRADEITNVVSRPQKVLTSERTASSTAYTISVSRVTENNTFDPAFPY
                     PANETMLILTLTIKNTSGGPQQLTPAQQLFVRSQDGDYAPMHASSYLATQLPAATVKP
                     GQTVSGQVSFAIPKSQGRPLLYVDLGWNNYGPAVFDVLH"
     gene            complement(1254..3722)
                     /locus_tag="VFH39_05145"
     CDS             complement(1254..3722)
                     /locus_tag="VFH39_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925187.1"
                     /translation="MDPVIVQINPPKTPFHMPHLPSMGPRGTRILTVAAALVICASML
                     LFATMSVLVNQGSFVRQHSIHNIDVPAIASGTQLSSAASPGGGIAVGTGGTSGTNSPV
                     VKLTATPASVAVGGSSVLKWSATNNPTSCSASGDWSGSRPASGSASTGSLNRVDTYIF
                     TLTCKTKTGTGFSTVSVGSVARGGTGNIATRPSVKLAATPSSVYTNESATLSWAVTNN
                     PTSCTASGDWSGARATTNSTWTVGPLSTAKTYTYTLTCQNGGGSGYATATVAATPPPP
                     NLPLTSLYANPVGPVTPGTNVQLTWKTTNNPTSCTASGDWSGSKSPSGGSQGTGPLND
                     LRTYTFNLSCRNNAGSTNDSAAIVVLPAPPSVSLTVSPSTINVGDSATISWSATNNPT
                     SCTASGDWSGSKAPNGGSQNTGVLNTARTYLYSLSCSNAGGTGYQNNVALTVGVPPAP
                     AVTLTASPITTTTGKSATLTWSATNNPTSCTASGSWSGSKSASGTQSTGALNSVKTYT
                     YKLACSNAGGTGQASASINVTSGGGTTSPPVVTVALNPTSIGAGSSSTISWSATNNPT
                     SCTASGSWSGSKSASGSQSTGAMGSAGSYTYSLSCSNSAGSGSASATLTVKAVPVVSV
                     TLSPVSITAGSSSTISWSATNSPSSCTAGGSWSGSKAASGSQSTGAMNTAGSFNFTLS
                     CTNSGGTGSGSGTLVVNPAGTVYCGGLSPCHGSAYMANHASVGNCYAWNTGSGSAYPW
                     IINITSFRPDHPGGTRTGSIEETTSVCNHNIHAILAGNAAIPGYKDSGGDTTYSHHSG
                     TVNNTSSSNLSSYRVGYYDAADPN"
     gene            complement(3728..4246)
                     /locus_tag="VFH39_05150"
     CDS             complement(3728..4246)
                     /locus_tag="VFH39_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925188.1"
                     /translation="MRTFLKTERQRHWAAALSVIAFLSLIPVAFDTTPAVPPHKPAPQ
                     SGITPGRHAVLGDSALRQQYGIKVISLNIGQTDQDSQNRHVTVTVSITNLSDQSMQVS
                     PGLEMNLRTSDGSLYPMTAEYLPAGTVVGGPVAAKETRAMDVDFKLPASAVPAAFVFQ
                     PDAASPATEVGL"
     gene            complement(4302..4871)
                     /locus_tag="VFH39_05155"
     CDS             complement(4302..4871)
                     /locus_tag="VFH39_05155"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET6925189.1"
                     /translation="MPKTSRTSKPKVDRPSRRDTWIRSLPVTRLFYASVILFVLAATL
                     LVLALASEHHQAQVRSAAAQSGYPLGTTLSAGVVTVRPVSVTYDPGEGVFEAPTGKEY
                     AIIDFSVMNRSAAPISVLPTADTYVKDAGGHVIYLTPDTLDAPFHAGELPPGETVRGQ
                     LSFLVPRSGPLKFYVDAIWSGGVLTYKLR"
     gene            complement(4888..5661)
                     /locus_tag="VFH39_05160"
     CDS             complement(4888..5661)
                     /locus_tag="VFH39_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925190.1"
                     /translation="MWLKRLVGVMMIIAFGGLNTLVVLGLFQPRHPHTPIVNPSDSPA
                     TVSNVAPTVNLSVGPSTVATGLTSAIQWTTTGTPDSCTASGDWSGDKTIYGAESTGRL
                     PKPGTYTYKLTCRNKIGSATASATVTVTNTPPATSRVATSTGISSSPAVTATYCSGRS
                     PCYGKADVARHSSAGDCWGYNLDRVIDISGFDLGYHIAKSGISSIRISGVCGTNLAPA
                     LDGSLSAGGQTRNHLASTKSNSDPNEIPYFVGFFDPNKP"
     gene            complement(5713..7122)
                     /locus_tag="VFH39_05165"
     CDS             complement(5713..7122)
                     /locus_tag="VFH39_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET6925191.1"
                     /translation="MQPAGDRPVIISFRIEHLLGWAVTFGLCLVPVVLWLQIHPLNTV
                     TGFVGIMLALGRVTGLIGMVMYALNLVYATRLRFLEFWFGGLNRVYIAHHLLGGFALI
                     FLTFHPLFLALRYVHTSLMQAALLLLPNGLGPLGALFNRHAELHQVVLNQWALFFGII
                     AFWGMVGLLLITFFVKVAYRFWLMTHKWLGVVFFIGGLHVLFISSDTSTDMALKTYIL
                     GITVVGLAAYVYRTLANRLVVKLHRYSVTQVVTAGGNVSQITLYPSDRPMRYRPGQFV
                     FVRFLGAEKVGVSDEWHPFSISSAPHETTLEISVKALGDYTNKLGRLPNGTIAEIEGA
                     YGRFSFNYFKNHNQVWIAGGIGVTPFISMARSLPPDNSYHVDLYYSIKTATEVIDWTA
                     LAIAANQHGGTLRVFPYIGDQMPGHLTTDYIEQYSGDLRGKDFFLCGPPPMMQSLRKQ
                     LRAKQVPGTSIHSEEFAMS"
     gene            7289..7936
                     /locus_tag="VFH39_05170"
     CDS             7289..7936
                     /locus_tag="VFH39_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET6925192.1"
                     /translation="MKTIIFDFDGTIADSFVLFVDILHKLMGRTERVSATELERLRGM
                     SLLQAAREVHVPWWRLPLLASRGRRMMLGAIDEVHPCQGMPEALKTLHADGYQLFIMS
                     SNSALNIDTFLKTHGLAGLIDKVYPGVSLFGKARTLRKVIRQNRFNRGDVMYVGDEVR
                     DIQGAKRAGIKVVSVTWGYNNADILERHAPDGLVYAAAELPDVVHKLQPLGDSAD"
     gene            7955..8740
                     /locus_tag="VFH39_05175"
     CDS             7955..8740
                     /locus_tag="VFH39_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6925193.1"
                     /translation="MANTYFRERSLPYKPGQKTPYKVSRSKIELFMQCPRCFWLDVRL
                     KISRPSSPPFNINKTIDELFKKEFDVHRQAGTPHPIMTANKLTGIVPFAHADMDKWRY
                     NFTGVVALHQPTNLHVFGAVDDVWVNEAGELIVVDYKATSKAKEVSIDSDWQISYKRQ
                     METYQWLLRQNGFTVSDTGYFVYTNARMDADGFGDQLSFTTKLIPYTGKDDWVEPTLA
                     KMKAVMDSDDMPPVGESIMGGPCEFCSYARARTELTMKHLRKS"
BASE COUNT         1721 a         2472 c         2914 g         1832 t
ORIGIN      
        1 aatgtcaaga agaaaatctg tggataaata tttttgtttc gacccaaaag agttgggtag
       61 ggcctgacca ttgcaaatgc ttgacgaaac cacctcgagc gtggctaaat caaagcaagg
      121 aagacgcgac ttgagcaaag aaacagtatc ggaactgcag ggccgccagg ctagtgaaca
      181 ggagctctat atgtatgagt gcgtcatcga aggcttgcac cggtcgatca tggccggaca
      241 ccggttcaca ggctatgata ccgaggtcat cggaaacatg gcgaccttgc gtatgcatga
      301 tcagccgcat cgctcgttgc aaatcagccg ggaagcggca accggtgtgt ggagcatcat
      361 cacaagatat cgctttgagg aagggggcga tacatccgac ggaacggaca cgtgggtgac
      421 cgaactccgg cccattgccc agcttgccgg gctgagcata gtcgagcgct gcgtagaggt
      481 accacggatg gcccgtcatc ccggcagggt attggcttcg gctacaaagg cgttgccgtc
      541 gtggtccgcc gtgcagtgct ggactggaac ccgtgaggca agggaactgc tggcgctcgg
      601 cacccatatg caggctagct gaggtttggt tacttggcga ctagtgcagg acgtcgaaga
      661 cggccggccc gtagttgttc cagccaaggt ccacgtacag cagcgggcgg ccctgggatt
      721 tcgggatggc gaacgacacc tggccgctga ccgtctgccc aggtttgacg gtggctgccg
      781 gcagctgggt ggccaagtag gaggaagcgt gcataggggc gtagtcgcca tcctggctgc
      841 ggacgaacag ctgttgggcc ggcgtaagct gttgcgggcc gccgctggtg ttcttgatgg
      901 tcagggttag gatgagcatc gtctcgttcg ccgggtaggg gaaggctgga tcgaacgtat
      961 tgttttcggt cacgcggctg acgctgatag tgtaggccgt gctgctggcg gtgcgctcgc
     1021 tggtcaaaac tttctggggt ctggaaacga cattggtgat ttcgtcggcc cggcccgcgg
     1081 ccgcgctctg cttgttcaaa atgtacgtgg tattgatgac gatgatcgtc agtaacacga
     1141 tgatgaaggt ggggaacagc agatgctcgt cgtcgcggat gttgacggct ttcagttgtt
     1201 gccagatcgt ggtctttcgt ttggctgttg ctttgcccat ggtttttacc cccttagttt
     1261 ggatccgccg cgtcgtagta gccgacgcgg taagaactta aattactgct gcttgtgttg
     1321 ttgacggtcc ctgaatggtg ggagtatgtg gtgtctccac cactgtcttt gtaaccggga
     1381 atggcggcgt tgccggccag aatggcgtgg atattgtggt tgcagaccga ggtcgtttct
     1441 tcgattgagc cggtacgggt gcctccgggg tgatcgggac ggaagctggt tatattgatg
     1501 atccagggat aggcgctccc gctgccggta ttccaggcat agcagttgcc cacgctggcg
     1561 tgattggcca tgtaggccga accgtggcag ggtgacaggc cgccgcagta gaccgtaccg
     1621 gcggggttga cgaccagggt gccggaaccg ctgccggtgc cgccgctgtt ggtgcagctg
     1681 agtgtgaagt tgaagctgcc ggccgtattc atagcgccgg tgctctggga gccgctggcg
     1741 gctttactgc cgctccagga accgccggca gtgcaggacg acggactgtt ggtggcactc
     1801 cagctgatgg tagaactgct gccggcagtg atcgataccg gactaagcgt gacgctcacc
     1861 accggcaccg ctttgaccgt cagggtggcc gacgcactac cacttcctgc gctgttgctg
     1921 cagcttagcg aataggtgta cgacccggca gaacccatag cgccggtact ttgtgatcca
     1981 ctggcgctct tgctaccgct ccagctccca ctggccgtac agctggtggg gttgttagtg
     2041 gcactccagc tgatggtcga actgctgccg gccccgatgg aggtcggatt aagagcgacg
     2101 gtcaccaccg gaggcgaagt agtgccgcct ccactggtaa cattgatgct ggcactggcc
     2161 tggccggtgc ccccggcatt gctgcaggct aacttatagg tgtacgtctt gacgctgttg
     2221 agcgcaccgg tgctttgggt gccgctggcg ctcttgctac cgctccagct cccactggcg
     2281 gtgcagctgg tgggattgtt cgtagcgctc caggtcagcg tggcgctttt gccggtcgtg
     2341 gtggtgatag ggctggcggt gagggtaacg gcaggtgccg gcggcacgcc gaccgtcaag
     2401 gcgacattgt tctggtagcc ggtgcccccg gcattgctgc agctgaggct gtacaagtag
     2461 gtgcgtgccg tgttgagcac gcccgtattc tgcgagccgc cgttgggggc tttgctaccg
     2521 ctccagtcgc cgctggccgt acacgacgtg ggattgttgg tggcgctcca gctgatggtg
     2581 gcgctgtcac cgacgttgat ggtgctcggg ctgactgtca ggctgaccga tggcggggcc
     2641 ggcaggacga cgatggcggc actgtcattg gtactgccgg cattgttccg gcatgacaga
     2701 ttgaacgtat aggtcctgag atcgttcagc gggcccgtgc cctgcgagcc gccgctcgga
     2761 ctcttgctgc cgctccagtc accgctggcg gtacacgacg tgggattgtt ggttgttttc
     2821 catgtcaatt gcacgttcgt accgggggtt accggtccca ctggattggc atagagggac
     2881 gtgagcggca ggttgggcgg tggaggtgta gcggcgaccg tcgcggtggc ataaccgctg
     2941 ccgccgccgt tctggcaagt cagggtgtag gtataggtct tggcggtcga aagcggaccg
     3001 acggtccagg tactgttggt cgtcgcccgg gcgccgctcc aatcaccact ggcggtgcag
     3061 ctggtgggat tgttggtgac cgcccagctc agcgtcgccg attcgttggt gtagaccgag
     3121 gacggggtag ctgccagctt gaccgatggc cgcgtggcga tgttgccggt accgccgcgg
     3181 gctaccgaac ctacgctcac cgtcgaaaac ccggtgccgg tcttcgtttt gcaggtcagc
     3241 gtgaatatat aggtatccac ccggttcaga ctgccggtag aagcgctgcc gctggccggt
     3301 cggctgccgc tccagtcacc actggcgctg cagctggtgg gattgttggt ggcggaccac
     3361 ttcaggaccg agctgccacc gacagccacc gacgccggtg tggctgtgag cttcacgacg
     3421 gggctgttgg ttccggaggt tccgcccgtg ccgacggcta tgccgccgcc cggcgaggcg
     3481 gcgctactaa gctgggtacc gctggctata gccgggacgt cgatattgtg gatgctatgc
     3541 tgccgcacga agctaccctg attgaccaat accgacatcg tggcgaacag cagcatactg
     3601 gcacagatga cgagcgccgc cgcgacggtg aggatgcggg tgccgcgcgg tcccatggac
     3661 ggcaggtggg gcatatggaa cggcgttttc ggggggttaa tctgaacgat gaccgggtcc
     3721 atgacggtta tagtcccact tccgtggccg gcgaagcggc atcaggctga aagacgaaag
     3781 cggccggaac ggccgaggcc ggcagtttga aatcaacgtc catggcgcgg gtttccttgg
     3841 cagcgaccgg accgccgacg accgtgccgg ccggcagata ctcggccgtc atcggataca
     3901 agctgccgtc gctggtccgc aggttcattt ccagaccggg agagacctgc atgctttgat
     3961 cggataggtt ggttatggaa acggtaacgg taacgtggcg gttttgcgaa tcctggtcgg
     4021 tctgaccgat gttcagcgaa atcaccttta tgccgtactg ctgacggagg gcgctgtcgc
     4081 ctaggacggc gtgccggccc ggagttatac cggactgtgg agctggttta tggggtggga
     4141 cagcgggggt ggtatcgaag gcgaccggga tgaggctcag aaaagcgatg accgacaaag
     4201 ctgcggccca atgtctctgg cgctcggtct tcaggaaagt cctcattccg ttactacccc
     4261 gtttcgctta agtatacccg atgccatgtc agtttccagc cttagcgcaa cttataggtt
     4321 agaacgccgc cgctccagat ggcgtcgacg tagaatttca gcggtccgga gcggggcacc
     4381 aagaagctca gctgaccgcg gacggtctcg ccgggcggca attcaccggc gtgaaaaggc
     4441 gcgtcgagcg tatcgggggt gagataaatg acgtggccgc cggcgtcctt cacgtatgta
     4501 tcggccgtgg gcaggacact gatgggggcg gccgaccggt tcatgacgct gaaatcgatg
     4561 atggcatact ccttgccggt cggcgcctcg aagacgcctt cgcccgggtc gtaagtgacg
     4621 ctgactggcc ggacggtcac cactcccgcc gagagggtag tgccgagcgg gtagccagat
     4681 tgggcagcag cggagcggac ttgtgcctga tggtgctcgc ttgccagcgc cagcaccagg
     4741 agcgtggcgg ccagcacgaa cagtatgacc gacgcgtaga agaggcgggt aacgggcaag
     4801 gaccgtatcc aggtatcgcg ccgacttggg cggtcaactt tgggtttgct ggttcgtgat
     4861 gtcttcggca tgatatatcc ttgtctgcta cggcttattc gggtcgaaaa atcctacgaa
     4921 gtaagggatt tcattcgggt cgctgttgct cttggtgctt gccagatgat tgcgcgtctg
     4981 accgccggcg ctgaggctgc cgtcgagggc cggtgccagg ttggtgccgc acacgccact
     5041 gatgcggatg ctgctgatgc cgctcttggc gatgtggtag ccgaggtcga aaccgctgat
     5101 gtcgatgacc cggtcgaggt tgtagcccca gcaatcaccg gcgctggaat gccgggcgac
     5161 atccgcctta ccgtaacagg gtgaacggcc gctgcaatag gtagcggtga cggccggact
     5221 gctgctgata ccggtggagg tagccacgcg gctggtagcc ggcggtgtat tggtgacagt
     5281 cacggtggcg gacgcggtag cgctgccgat tttgttacgg caggtcagct tataggtgta
     5341 cgtgccgggt ttaggcagcc ggccggtact ctcggcccca tagattgtct tatcgccgct
     5401 ccagtcaccg ctggcggtgc agctgtcggg ggtgccggtg gtggtccact ggatggcgct
     5461 cgtcagaccg gtcgcgacgg tcgaaggtcc gacgctcagg ttgacggtcg gtgccacatt
     5521 gctgacagta gccggggagt cggaaggatt gacgatgggg gtatgcggat gccgcggctg
     5581 gaacaagccc agcaccacca gcgtgttcaa gccgccgaag gcgatgatca tcatcactcc
     5641 caccagtcgc ttcagccaca tcagtggttt cctccggtca gtggtggggt aggctgtctc
     5701 attatcattt ccttatgaca tcgcgaattc ttcgctatgg atgctggttc ccggtacttg
     5761 ctttgcccgc agctgcttgc gcagcgattg catcatcggc ggcggtccgc acaaaaagaa
     5821 gtctttgccg cgcaggtcgc cgctgtactg ttctatataa tcggtggtca ggtgacccgg
     5881 catctggtcg ccgatgtacg gaaagacgcg caacgtgccg ccgtgctggt tggcagcgat
     5941 ggccagggcg gtccagtcga tgacctcggt ggccgtcttg atggagtaat acaggtcgac
     6001 gtggtagctg ttgtcgggcg gcaggctgcg ggccatcgaa atgaacggtg tcacgccgat
     6061 gccgccggcg atccatacct gattatggtt cttgaagtaa ttgaagctga agcggccgta
     6121 cgcgccctcg atctccgcga tagtgccgtt gggcagccgc cctagcttgt tcgtgtaatc
     6181 gcccagggct ttgacgctga tttccagcgt ggtctcatgg ggagcggagg aaatcgaaaa
     6241 cgggtgccac tcgtcactga caccgacttt ctcggccccc aaaaagcgga cgaagacgaa
     6301 ctggccgggc cggtaccgca tcggcctgtc gctggggtag agcgtaatct gcgacacgtt
     6361 gccaccggcc gtcacgacct gggtgacact gtagcggtgt agcttcacca ccaaccggtt
     6421 ggcgagcgtg cgatacacat acgccgccag cccgacgact gtgatgccca ggatatatgt
     6481 cttcaaagcc atatcggtgg aggtgtcact gctgatgaac agcacgtgca gcccgccgat
     6541 gaaaaagacg acgccgagcc acttatgcgt cataagccag aagcggtagg caacttttac
     6601 gaagaaggtg ataagcagca gcccgaccat cccccagaaa gcgatgatac cgaaaaacag
     6661 cgcccactgg ttcagcacta cctggtgcag ttcggcatgc cggttgaaga gggcgcccag
     6721 cggtccgagt ccgttcggca atagcagcag cgcggcctgc atgagcgacg tatggacata
     6781 ccgcagtgcc aggaacagcg ggtggaacgt cagaaagatc agcgcgaaac cgccgagcaa
     6841 gtggtgggcg atgtagacgc ggttcaggcc gccgaaccag aactccagaa accgcagccg
     6901 ggtggcatag accaggttga gcgcgtacat caccataccg ataagtccgg tcaccctgcc
     6961 gagcgccagc atgatgccga cgaacccggt gacggtgttg agcgggtgga tctgtagcca
     7021 cagcaccacc ggtaccaggc acaggccgaa cgtcaccgcc catccgagca gatgctcgat
     7081 gcggaagctg atgataaccg gtcggtcacc tgcgggttgc acggcgcgct tgtcctcggg
     7141 cgttacagca cctatctatt acgcctcatt ttctatgaaa catcggctaa acacaagcgt
     7201 ttcgttgcga tttcaggact acggggatgt gattgtgcgc acatattttt cacaccgctg
     7261 gtgctcaccg gtatgctata gtttcggggt gaagaccatt atctttgatt tcgacggcac
     7321 tatcgccgac agtttcgtac tgtttgtcga catattgcac aagctgatgg gccgcaccga
     7381 acgcgtgtcg gccaccgagc tggagcgtct gcgcggcatg tcgctgctgc aagccgcccg
     7441 ggaagtccac gtgccctggt ggcggctgcc gctgctggcc tcacgcggcc gccgcatgat
     7501 gctgggggct atcgacgagg tacacccctg ccagggcatg cccgaagcgc tgaagacctt
     7561 acacgccgat ggttaccagc tgttcatcat gagcagcaac agcgccctga acatcgatac
     7621 ctttctgaag acccacgggc tggccggcct tatcgacaaa gtttatccag gcgtcagctt
     7681 attcggcaag gcacgcacct tgcgcaaggt catccggcag aaccgcttta accgcggtga
     7741 cgtcatgtat gtaggcgatg aagtccgcga tatccagggg gccaagcggg ccggcatcaa
     7801 ggtcgtttct gtcacctggg gctacaacaa cgccgacatc ctggaacggc atgcgcccga
     7861 cggtctggtg tacgccgccg ccgagctgcc ggacgtcgtg cataagctgc agccgctcgg
     7921 cgacagcgcc gactgagttg ctatacttat ataaatggcg aatacctatt tccgggagcg
     7981 cagcctgccg tacaaaccag ggcagaagac gccgtacaaa gtctcgcgca gcaagatcga
     8041 gctgttcatg cagtgcccgc gctgcttctg gctcgatgta cgcctgaaga tcagccggcc
     8101 gtcgagcccg ccgttcaaca tcaataagac tatcgatgaa ctgttcaaga aagagttcga
     8161 cgtgcaccgc caggccggca cgccgcaccc catcatgact gccaacaagc tgaccggtat
     8221 cgtgccgttc gcccacgccg acatggacaa gtggcgctac aacttcaccg gcgtggtggc
     8281 gctgcatcaa ccgaccaatc tgcacgtttt cggggcggtc gacgacgtct gggtgaacga
     8341 ggccggtgag ctgatcgtcg tcgactacaa ggcgaccagc aaggctaaag aagtgagtat
     8401 cgacagcgat tggcagatca gctacaaacg gcagatggaa acctaccagt ggctgctccg
     8461 ccagaacggc ttcacggtga gtgatactgg ctatttcgta tacacaaatg ctcgtatgga
     8521 cgcggacggt ttcggcgatc agttgtcgtt cacgaccaaa ctcatcccgt ataccggcaa
     8581 agacgactgg gtcgaaccga cgctcgccaa aatgaaagct gtcatggaca gtgacgacat
     8641 gcccccggtg ggggagagca tcatgggcgg tccctgcgag ttctgcagct acgcccgcgc
     8701 ccgtaccgag ctgaccatga aacacctccg taagagctga acattgcaga tttgcaatta
     8761 gctggattaa gtataaaata attattcaat gacagcagcg gagttcacgg attgccgact
     8821 tgttgatgta aatgggcagg cgctgccgcg ggtcttcggg cacgagcagg agtatggctt
     8881 taatttcatg gggcgtgaac agcacgaaga tatccagttc accgagttat tgctccgca
//
LOCUS       DASSFD010000214         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1128871, whole genome shotgun sequence.
ACCESSION   DASSFD010000214 DASSFD010000000
VERSION     DASSFD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128871"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFH39_05180"
     CDS             <1..653
                     /locus_tag="VFH39_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925194.1"
                     /translation="IEEEAKAMAGSPLLTTDEFEDAIREVFPFDESTLDRLFDTEADK
                     FEPVLVTAAKELYDARTEAFTPEIMHKIEHDIYLQVLDELWMQHLENMDHLREGIHWM
                     SVGQQDPLVEYRRRGQQLFDAMQQDLRHTVLRTLFHAQPIDMSMLEQPIETELTRAAR
                     GSISNAGQINNDQEQFDETSFVPKKTETQVKKQVATKRKATRKTERKRKTTARRRK"
     gene            758..2053
                     /locus_tag="VFH39_05185"
     CDS             758..2053
                     /locus_tag="VFH39_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6925195.1"
                     /translation="MKHTVTEISLKNGAKGLLVHIPDASVMTFDLNFRAGEYLVDPKK
                     WEVPHLMEHVLLGANELIPKARDFQAELEKNGAYSNATTGVYDITYEAECADFEWDRV
                     LGLLLIAITKPLFLEEEFKAEFGNVQEEMVARANNHFRRLSLEMRRALGLIVRTDTER
                     LELMGNVTVQDVREHYFATHHTQNLRFVIAGNLNRKRRAVVENLFENIELPQKGKRFP
                     LPKEQPKSLRKPVYVHNDAVENMYFYIDTFMNRRMSDPETDALNLINTMLTETLYSKI
                     LGTARERGLVYGMSSGLSQTREASNWWFGAQVSDKNAQALFKIVIDELNKVMRGEIAT
                     SEIETTKQYALGRFQRSAQTVGGTANGYAGRYFFDDVIEDYYRIPERIKAVEKQQIVT
                     IARQLFADKVWGFGVLGDCGEEFAGELRSQLAPLWQTKE"
     gene            2293..>2786
                     /locus_tag="VFH39_05190"
     CDS             2293..>2786
                     /locus_tag="VFH39_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HET6925196.1"
                     /translation="MEDLLPQATQLQAEIQDATERLKLFALEKELDELQAQSQQPDFW
                     QDNLAAQAVMKQIAKLEARVKPWRELLDAADELIDIIHLDVEGSEQEVTQQLDWITNK
                     FDDYKEQLKLNGPYDDHDAILSIHAGAGGTDAQDWTQMLFRMYQRYAEKSGWQTQLIE
                     ESPG"
BASE COUNT          694 a          821 c          784 g          487 t
ORIGIN      
        1 tcatcgagga agaagccaag gcgatggctg gctcgccgct cttgaccacc gacgagttcg
       61 aggacgccat ccgcgaagtc tttccgttcg acgagtcgac actggaccgt ctgttcgaca
      121 ccgaggccga caagttcgaa ccggtgctgg taaccgccgc caaggaactg tacgacgccc
      181 gcaccgaagc cttcacgccg gaaatcatgc acaagattga acacgatatc tatctgcagg
      241 tgctcgacga gctgtggatg cagcacctcg agaacatgga tcacctgcgc gaaggcatcc
      301 actggatgag cgtcggtcag caggacccgc tggtcgagta ccgtcgccgc ggtcagcagc
      361 tgttcgacgc catgcagcag gacctgcgcc acaccgtgct gcgcacgctg ttccacgccc
      421 agccgatcga catgagtatg ctggaacagc ccatcgagac cgagttgact cgcgctgccc
      481 gtggcagtat cagtaacgcc ggccagatca ataacgacca ggagcagttc gacgaaacca
      541 gcttcgtccc caagaagacc gagacgcagg tcaagaaaca ggtggccacc aagcgtaagg
      601 ccacccgcaa gaccgaacgt aaacgtaaaa caaccgcccg tcggcgcaag tagggaacca
      661 aagccatcat atgctgagta gtatttcttt cacccacgtc caccagctat tcgtaatgct
      721 caccaaattc caacctttaa accccgcctt ctaacctatg aaacacactg tcacagaaat
      781 cagtcttaaa aacggtgcca agggactgct ggtgcacatc ccggacgcca gcgtcatgac
      841 gtttgacctc aacttccgcg ccggcgaata cctggtcgac ccgaagaagt gggaagtgcc
      901 gcacctgatg gagcacgtgc tgctcggtgc taacgaactg attccgaaag cccgcgactt
      961 ccaggccgaa ctcgaaaaga acggtgccta cagcaacgcc actaccggcg tgtacgacat
     1021 cacctatgaa gcggagtgtg ccgattttga gtgggaccgg gtgctcgggt tgttgctaat
     1081 cgccattacc aaaccgctgt ttttggaaga agagttcaag gccgagttcg gcaacgtcca
     1141 ggaagaaatg gtcgcccggg cgaacaatca cttccgccgt ctgtcgctgg agatgcggcg
     1201 ggccctcggt cttatcgtgc gcaccgatac cgaacgcctg gagctgatgg gcaacgtaac
     1261 ggtgcaggac gtccgcgagc actacttcgc cacccatcac acgcagaacc tgcgcttcgt
     1321 catcgcgggc aacctcaacc gcaagcgccg ggcagttgtc gagaacctgt tcgagaacat
     1381 cgagctgccg cagaagggca agcgtttccc gctgcccaag gaacagccga aaagcttacg
     1441 caagccggta tacgtgcata acgacgctgt cgaaaatatg tacttctaca tcgacacgtt
     1501 catgaaccgt cgcatgagcg acccggagac agacgccctg aaccttatca acaccatgct
     1561 gaccgagacg ctgtacagca aaatcctcgg caccgcccgc gaacgcggcc tggtgtacgg
     1621 catgagcagc gggctttccc agacccgcga ggccagcaac tggtggttcg gtgcgcaggt
     1681 gtccgacaag aacgcgcagg ccttattcaa aattgtcatt gatgagctga acaaagtgat
     1741 gcgcggcgag atcgccacgt ccgaaatcga gaccaccaag cagtacgcgc tcggccgttt
     1801 ccagcgcagc gcgcagacgg tcggcggcac ggccaacggc tatgccggcc gctacttctt
     1861 cgacgacgtc atcgaagatt actaccggat tccggaacgc atcaaagcgg tcgaaaagca
     1921 gcagatcgtc actatcgccc ggcagctgtt cgccgacaaa gtctggggct tcggggtact
     1981 tggcgactgc ggtgaagagt tcgccggcga gctacgcagc cagttggcgc cgttatggca
     2041 gactaaggaa taagtcctat ccttactttt ggcttgaacc aaaagtaagc aaaagttcaa
     2101 ggcccgaacg taaccctcgg tcgatagttg tggcagctta caaccggctc gaggactcgc
     2161 tgcgctcaaa catctcgagc cgtccctagc gggaaagttg taattgccag caacaatcga
     2221 gccgggtgct actttagggc cgttccaaca catgagttgg aaagagttat agaggagcat
     2281 acaatagggg tgatggaaga cttactgcca caagccaccc agctgcaagc agagatccag
     2341 gacgccaccg agcgtctgaa gttgttcgcg ctggaaaaag aattggatga gttgcaggcg
     2401 cagagccagc agccggattt ctggcaggac aacctggcgg cgcaggccgt catgaagcag
     2461 atcgctaagc tcgaggcgcg ggtcaagcca tggcgcgagc tgctggatgc cgccgatgag
     2521 cttatcgaca tcattcactt ggacgttgag ggcagcgagc aagaagttac gcaacagctg
     2581 gattggatta ccaataagtt cgacgactac aaagagcagc tcaaactcaa cgggccgtac
     2641 gatgaccacg atgccatcct cagcatccac gccggggcag ggggcaccga cgcccaggac
     2701 tggacgcaga tgctgttccg catgtatcag cgctacgctg agaagtccgg ctggcagacg
     2761 caacttatag aggaatcacc tggtga
//
LOCUS       DASSFD010000215         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1867195, whole genome shotgun sequence.
ACCESSION   DASSFD010000215 DASSFD010000000
VERSION     DASSFD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867195"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFH39_05195"
     CDS             <1..195
                     /locus_tag="VFH39_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925197.1"
                     /translation="SDTITIAVQVNGKLRGEFQAATGVNEADAVATAKTQERVKPHIE
                     GKEIVKAIYVPGKLVNLVVR"
     gene            294..482
                     /locus_tag="VFH39_05200"
     CDS             294..482
                     /locus_tag="VFH39_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925198.1"
                     /translation="MGKPKKRTSPRRTGTRRSHLVVKLARAVNAKSPVKAYTTRRESG
                     SSSPKAEPAKTATKSKTS"
     gene            542..1072
                     /gene="nusB"
                     /locus_tag="VFH39_05205"
     CDS             542..1072
                     /gene="nusB"
                     /locus_tag="VFH39_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6925199.1"
                     /translation="MASNRHLGRIIALQTLYELDFRRDANDTELKFDDILTRNIARYK
                     DMVDDVDFVRQLVSGVDAQSKALDERLQPIAPEWPIDQIARMDRVVLRIGLYELENEK
                     DVPPKVVINEAVELAKAFGGENSSKFINGVLGTALRQLNGETEPTTAKPAVKPKPARK
                     TPTKKKSTTKQEAPDA"
     gene            1082..1669
                     /locus_tag="VFH39_05210"
     CDS             1082..1669
                     /locus_tag="VFH39_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6925200.1"
                     /translation="MPNADKPAPKQPTPSGKPDGAADRSEKRGFKLKRPRLPGIPKFV
                     KRRPVIEEVVRETTSGGVVFRRNQKTNQLEILLIQDAKNRWTIPKGHVEEGEEPKDTA
                     EREIREETGLQEMKVYNWLGKVNFRYRRSHTLVLMTMHIYLVEGLGKTDELQGEDWLT
                     DIRWLPATDAVDKIAYEDIGKLMLVGMKKVREMKA"
     gene            1767..2474
                     /gene="rnc"
                     /locus_tag="VFH39_05215"
     CDS             1767..2474
                     /gene="rnc"
                     /locus_tag="VFH39_05215"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET6925201.1"
                     /translation="MKMDTTKYQDFAKQKLGVTFNDIQLLVTAFTHRSFLNEHRTTAT
                     EHNERLEFLGDAVLELVSTKYLYLNFSEPEGILTNWRSSLVRTESISAAASRYDFEPL
                     LRLSRGERHGTERARMQILANSYESVVGALYLDQGYEVAERFITESLLVTFKDILETG
                     SWMDPKSHLQEQVQSQEGQTPQYKILSEEGPDHDKTFTVGVFVGDSLRGQGSGPSKQA
                     AQQKAAESALAKLKKSA"
BASE COUNT          666 a          764 c          711 g          424 t
ORIGIN      
        1 agtgatacca tcaccatcgc cgtgcaagta aacggcaagc tgcgcggtga attccaagcc
       61 gctaccggtg tgaatgaagc tgatgcggtg gcgaccgcca agacgcagga acgggtcaag
      121 ccgcatattg agggcaaaga aatagtcaaa gccatctacg tccccggcaa actggtcaac
      181 ctggtggtcc gctaagcccc gaggtcttgt gttctaaggt gttatcgggt ataatcagac
      241 gcaatatcgt cataatacag ctgcactaaa agcagacagg agtcagtcga aacatgggta
      301 aacccaagaa acgaaccagt ccgcgccgta ccggtacgcg ccgcagccac ctggtggtca
      361 aactggcccg ggccgtcaac gccaagtcgc cggtcaaggc ttacaccact cggcgcgaat
      421 ccggcagctc cagccccaag gctgagccgg ccaagaccgc aaccaaaagc aaaaccagct
      481 agtattcccg cctccgggcg ggctatactg gcttgtaagg gcaaatcgga aagttaacaa
      541 tatggcatcc aaccgacacc ttggccgcat catcgcattg cagacgctgt acgagctgga
      601 tttccggcgg gatgccaacg acaccgagct gaaatttgac gatatactga cgcgcaacat
      661 cgcgcgttac aaagatatgg tcgacgacgt agacttcgtc cgccagctgg tcagcggcgt
      721 cgatgcccag agtaaggcgc tcgacgaacg tttgcagccg attgcgcccg agtggccgat
      781 cgaccagatc gcccggatgg accgggtagt cttacgcatc gggctgtacg agctcgagaa
      841 cgagaaagat gtgccgccca aggtggtcat caacgaagcc gtcgaactgg ccaaggcctt
      901 cgggggcgag aactccagca agttcatcaa tggcgtgctc ggcaccgccc tgcgccaatt
      961 gaacggcgag acggaaccga cgaccgccaa acctgccgtc aaaccgaaac ccgctcgcaa
     1021 gactccgacc aaaaagaaat caactactaa gcaagaagcg cctgacgctt aaaaggaatc
     1081 cgtgccgaac gcagataaac cagcacccaa gcagcccacg ccgtccggca aaccggatgg
     1141 ggctgccgac cgttccgaaa aacgcggctt caagctgaag cgtccgcgcc tgcccggcat
     1201 tcctaagttc gtcaaacgcc ggcccgtcat cgaggaagtg gtacgcgaaa cgacttccgg
     1261 cggcgtcgtt ttccgccgca accaaaagac caatcagctc gagatcctgc ttatccagga
     1321 tgccaagaac cgctggacca ttccgaaagg gcatgtcgaa gagggcgagg agcccaaaga
     1381 caccgccgag cgcgagattc gcgaagagac cggtctacag gagatgaaag tctacaactg
     1441 gctcggcaaa gtgaacttcc gctaccgacg cagccacacg ctggtgctca tgacgatgca
     1501 catctacctg gtcgaaggcc tcggcaagac cgacgaattg cagggcgagg actggctgac
     1561 cgacatccgc tggctgccgg cgaccgacgc cgtcgacaag atcgcctacg aggacatcgg
     1621 caagcttatg ctggtcggca tgaagaaagt tcgcgaaatg aaggcttgaa aggagccgca
     1681 cgtacaccgg ttgatagttg ctagtttcca ggtgaacctt tagaagccaa gctgcagact
     1741 aagagctata aaccaagcac aaccatatga agatggacac caccaaatac caggacttcg
     1801 ccaagcaaaa acttggcgtg acgttcaacg acatccagct gctcgtcacg gcgtttaccc
     1861 accgctcgtt cctgaacgag caccgcacca ccgccaccga acacaacgag cgtctcgaat
     1921 ttctcggcga tgccgtgctg gagctggtct cgaccaaata cctctacctc aacttcagcg
     1981 agccggaagg catcctgacc aactggcgca gtagcctggt gcgcaccgaa agtatcagcg
     2041 ccgccgcgtc gcgctatgac ttcgaaccgc tgttgcgctt gagccgcggt gagcgacacg
     2101 gcaccgagcg cgcccgcatg cagattctgg ccaatagtta cgagtccgtc gtcggtgcac
     2161 tgtatctgga ccagggctat gaggtcgccg agcggtttat taccgagtcg ttgctggtga
     2221 cattcaaaga cattttggag accggttcct ggatggaccc taaatcgcac ctgcaggagc
     2281 aggttcagag ccaggaaggt cagacgccac agtataagat tctgagcgaa gaaggacccg
     2341 accacgacaa gacctttact gtcggtgtat ttgtcggcga cagcctgcga ggtcagggca
     2401 gcggccccag caagcaagcc gcccagcaaa aagcggccga gtcggccctc gccaagctca
     2461 agaagtcggc ctgacgcccc gccgccggtt tgacaacaga ggcggtaacc tgtaaaatac
     2521 cctggattgt ctttataaac ctttaataac gtagagacaa aggag
//
LOCUS       DASSFD010000216         7322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1252589, whole genome shotgun sequence.
ACCESSION   DASSFD010000216 DASSFD010000000
VERSION     DASSFD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252589"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFH39_05220"
     CDS             complement(<1..554)
                     /locus_tag="VFH39_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6925202.1"
                     /translation="MAEKRDYYEVLGVGKNASADEIKKAFRRKAVELHPDKQGGDEAK
                     FKEVNEAYEVLKDPSKKQRYDQFGHAGVGGNGGGFDFNGFGGFGQGGQSFDFGDLGLG
                     DIFSSFFGGGMGGQQQRRQARGRDIEARVEISFEQAIFGTEVELQLNMEDTCEHCKGT
                     TVEPGYELKTCDTCGGSGQVVSVAR"
     gene            complement(617..1216)
                     /locus_tag="VFH39_05225"
     CDS             complement(617..1216)
                     /locus_tag="VFH39_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925203.1"
                     /translation="MKSPETPVLADLGTELATQEEIGWLACRLLEAKRQAGMFVTNYV
                     DGSMVSDKTLEFRVNDTEVICGIPGLVVRYPQISIETRERFAQTPGGGQVDVRIRDKL
                     KNASRLRNRPAYYCLAGTPEYVRGNRVLRTPRRLYTNPWEVIDASPEAREDDEKKFVA
                     AIGPRSGPNLSKERYAQLHYLVEAILAGTAEPLHKKALE"
     gene            complement(1264..3180)
                     /gene="dnaK"
                     /locus_tag="VFH39_05230"
     CDS             complement(1264..3180)
                     /gene="dnaK"
                     /locus_tag="VFH39_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6925204.1"
                     /translation="MAKIIGIDLGTTNSAMAVMQAGKPEIIANSEGNRTTPSVVAVNK
                     NGERLVGQVARRQQVTNPKNTIYEVKRLIGRNFSDKEVQRDLKLMGYEIVKAGNGVKV
                     KMADKEYSPEEISAMVLGKLKADAESFLGDKVTEAVITVPAYFDDSQRQATKDAGKIA
                     GLEVKRIVNEPTAAALAYGLDKEGKDEKIAVYDLGGGTFDVSILELGDGVFEVKATNG
                     DTHLGGADFDRTLVNYFVDEFKKDSGIDVSSDNAAMQRLRDEAEKAKIELSTAQEVDI
                     NLPFLTADAEGPKHFEHKLTRAKLESLVADLIDKTASPCEKALKDAGLKASDIDAVVL
                     VGGMTRMPAVQEKVKKIFGKDPMKGVNPDEVVAIGAAIQGGVLQGDVKGVLLLDVTPL
                     SLGIETEGGLRNVLIERNTTIPTSKSQVYSTASDNQPQVEINVLQGEREMAADNKSLG
                     RFILDGIPPAPRGVPQIEVTFNLDANGILNVTAKDKGSGKEQNITIQGSGSLDKAEVE
                     KMAKDAEAHADEDKKKREAIEARNMLDSAIYQAEKMKSDNKDKIADDDVKKIDEAVEK
                     AKKVAADDKADKEALEAASKELSDVLMPIGAKMYENASAGETPDEDKAADDSKSEDKK
                     DDGPIEGEVVDDKK"
     gene            complement(3201..3452)
                     /locus_tag="VFH39_05235"
     CDS             complement(3201..3452)
                     /locus_tag="VFH39_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925205.1"
                     /translation="MEEYLPKTSWRQKAKRIGGTIFSSARHSFVMSPPVRSGEAFSPL
                     QAAAGHFREQLPRITELVELDTSLKDIAAEIPDDNPNQE"
     gene            complement(3768..4256)
                     /locus_tag="VFH39_05240"
     CDS             complement(3768..4256)
                     /locus_tag="VFH39_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6925206.1"
                     /translation="MTDKTKKPAKHHDNDELEQLEQQVAELTDALQRERADVINVRRR
                     HEEQLAGLKNVVKSGVIRDLLPIIDNFERALKHVPKDLEGNDFVKGVQGVVKQFEKTL
                     DGLGVERIKTVGEPFDPKYHEAISMEDGDGTNEVVSEELQAGYRLGDEVIRHAMVRVK
                     MR"
     gene            complement(4425..5129)
                     /locus_tag="VFH39_05245"
     CDS             complement(4425..5129)
                     /locus_tag="VFH39_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET6925207.1"
                     /translation="MTERQKQILSAIIEQYAEVAVPVGSSLLAKVFDVSSATIRAEMA
                     ELERLGYIRQPHTSAGRIPTDRGYRFYVNNLQESTALSAPERRAERALTARVTHGGLP
                     ERTIRNAVDTLVELTHNLGLATIGNQLYISGLSNLFGQPEFLSSTQQVQQVANLLDNL
                     EPWLREAAPNEPLSVFIGSENPIGRSAGVSLIISRFRSPFSDHSYIGTLGPTRQSYRD
                     VMALVAHTGKTLEEVL"
     gene            complement(5187..5912)
                     /locus_tag="VFH39_05250"
     CDS             complement(5187..5912)
                     /locus_tag="VFH39_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925208.1"
                     /translation="MNQLTRIDEFRQVLADYHVSDEARSILAKTKLVLLVGPSSSGRN
                     TIINELLTTGDYHYIVSDTTRKPRINNGVPEQNGREYWFRTEDELLADLQAGMFLEAA
                     IIHNQQVSGISIRELSAAAAEGKIAINEIEVVGGENIHAVKPDALFLFIVPPSFDEWM
                     TRMKARGELPEDETRRRLESAVQELQVALAHDYYRFVVNDTFKHTARDIHAMSQGLDV
                     PDDDEARAIAEQLLHDTQQFLGR"
     gene            complement(5973..6398)
                     /locus_tag="VFH39_05255"
     CDS             complement(5973..6398)
                     /locus_tag="VFH39_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925209.1"
                     /translation="MFQLSRQILRITVLTIGFILFVVVTVHSATQATNREAVVGKPSL
                     FTISQLGASSTLAAANLSGPNSMAPDAVNVPQPAGTHVATTQTGEMTQTMASPAATPP
                     APAKEHAAASLPQAGQLPPTAAPQAVAATAKAAAQHQLP"
     gene            complement(6805..>7322)
                     /locus_tag="VFH39_05260"
     CDS             complement(6805..>7322)
                     /locus_tag="VFH39_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641513.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET6925210.1"
                     /translation="KRGLQKNLSYVDQRAVVSFEAPLANLLTDFYDQLKSLTSGYGSF
                     NYELADYRAEDLVRVDFYVAGDMVSALRVMVHRSEAERLGRETVQKLKEIIPRQNFQV
                     ALQAAIGGRFIAREDISAYRKDVTVGLYGGDVSRKKKVLAKQAKGKKRMKRFGKVDIP
                     ADAFTVMLKRD"
BASE COUNT         1283 a         2161 c         2059 g         1819 t
ORIGIN      
        1 cgggcaacgc tcactacctg tccggaaccg ccacaagtgt cgcaagtttt cagctcataa
       61 cctggctcga cagtcgtacc cttgcagtgt tcgcacgtgt cttccatgtt caactgcagc
      121 tcgacctcgg tgccaaaaat cgcctgctcg aaactgattt ctacccgggc ctcgatgtcg
      181 cggccccggg cctgacggcg ttgctgctga ccgcccatac cgccgccgaa gaaactggag
      241 aaaatatcgc ctaagcccaa gtcgccgaag tcgaagcttt ggcccccctg gccgaaaccg
      301 ccgaagccgt tgaagtcgaa gccgccgcca ttgccgccga caccggcgtg gccgaactga
      361 tcgtagcgct gcttcttgct cgggtccttc aggacctcat acgcttcgtt cacctctttg
      421 aacttggcct cgtcaccgcc ctgcttgtcc gggtgcagct ccacggcctt ccgacggaag
      481 gcctttttta tctcgtcggc gctggcgttc ttgccaacac ccaaaacctc gtaataatcg
      541 cgtttctcag ccataatcac ctacatttta gcactaaaca cctacgagtg ctaacgctcg
      601 ttgttggcgc cggcggctat tccaatgctt ttttgtgcaa cggctcggcc gtgcccgcca
      661 atatggcctc taccaggtag tgaagctggg cataccgttc cttgcttaga ttcggtccgc
      721 tgcgcggccc gatggcagcc acaaatttct tctcatcatc ttcgcgggcc tcgggcgatg
      781 cgtcaatcac ttcccacggg ttcgtataca gacggcgtgg ggttcgcagc accctgttac
      841 cccgcacata ttccggcgta ccggccaggc aatagtaggc gggccggttc cttaagcggc
      901 tggcgttctt gagcttgtcc cggattcgta catctacctg gcctccgcct ggcgtttgcg
      961 cgaacctttc ccgcgtctcg attgatatct gcggataccg gacgacgagc ccggggatac
     1021 cgcaaataac ctccgtatcg ttgactctga actccagagt tttgtcgctc accattgaac
     1081 cgtctacgta atttgtcacg aacataccgg cctgacgttt ggcttcgagc agacggcagg
     1141 ctaaccaccc tatctcctcc tgggtcgcca attcggtgcc cagatctgcc aaaaccggtg
     1201 tttcgggaga tttcataacg tggctagtat gctccagaca taccgattag tccagcgggg
     1261 cgcttacttc ttgtcgtcga cgacttcgcc ttctatgggg ccatcgtctt tcttgtcttc
     1321 ggacttgctg tcgtcagcgg ctttgtcttc gtccggggtc tcgccggcgg aagcgttctc
     1381 gtacatcttg gcgccgatgg gcatgagcac gtcgctcagc tctttgctgg ctgcttccag
     1441 ggcttctttg tcggctttgt cgtcggcggc tactttctta gccttctcga cggcttcgtc
     1501 gattttcttt acgtcgtcgt cagcgatctt gtccttgttg tcgctcttca tcttttcggc
     1561 ctggtaaatg gcgctgtcga gcatattacg ggcctcgata gcttcgcgct tcttcttgtc
     1621 ctcatcggcg tgagcctcgg cgtccttggc catcttttcg acttcggctt tgtcgaggct
     1681 gccgctgccc tggatggtaa tattctgctc tttgccggag cctttgtcct tcgcagtcac
     1741 gttcaggatg ccgttggcgt cgaggttaaa ggtaacttcg atctgcggta cgccgcgcgg
     1801 tgcgggcggg atgccatcga ggatgaagcg gccaagactc ttgttgtcgg cggccatttc
     1861 gcgctcaccc tgcagcacgt tgatttccac ctgcggctgg ttgtcgctgg cggtactgta
     1921 cacctggctc ttgctggtcg ggatggtggt gttgcgctcg attagcacat tgcgcagacc
     1981 gccttcggtt tcgatgccga ggctcaaagg cgtcacgtcc agcagcagca cgcccttgac
     2041 gtcaccctgc agcacaccgc cctggatggc agcgccgatg gccacgactt catccgggtt
     2101 cacgcccttc atcgggtcct tgccgaagat tttcttgacc ttctcctgca cggccggcat
     2161 gcgggtcata ccacccacca gcactaccgc gtcgatgtcg ctggctttga ggcctgcgtc
     2221 cttcaaggct ttttcgcacg ggctggccgt cttatcgata aggtcggcca ccaggctctc
     2281 aagtttggca cgggtcaact tgtgctcgaa gtgcttcggg ccttcggcat cggcggtcaa
     2341 aaacggcagg ttgatgtcga cttcttgcgc cgtagacagc tcgatcttag ccttctccgc
     2401 ctcgtcgcgc aggcgctgca tagcagcgtt atcgctggaa acgtcgatgc cgctgtcctt
     2461 cttgaattcg tccacgaagt agttcacgag cgtacggtcg aagtcggcac caccgaggtg
     2521 cgtgtcaccg ttggtcgcct tcacttcgaa tacgccgtcg cccagctcca aaatagatac
     2581 gtcgaatgta ccgccaccca ggtcgtacac ggcgattttc tcgtccttgc cttctttgtc
     2641 caaaccatat gccagcgcag ccgcggtcgg ctcgttcaca atacgcttta cttcgagacc
     2701 ggcgatctta ccggcgtctt tggtagcctg gcgctgagag tcgtcgaagt aagctggcac
     2761 ggtaatgacg gcttcggtga ccttgtcacc caagaaactt tcggcgtcgg ccttcagttt
     2821 gccgagcacc atggcgctga tttcttccgg gctgtactct ttgtcggcca tcttgacctt
     2881 cacgccgttg ccggctttga cgatctcgta acccatcagc ttgaggtcgc gctggacttc
     2941 tttgtcgctg aagttacggc cgatgaggcg cttcacttca taaatggtgt tcttggggtt
     3001 ggtgacttgc tggcggcgtg ccacctggcc taccaggcgc tccccgttct tgttcacggc
     3061 tacgacggat ggcgtggttc ggttgccctc actgttcgcg ataatctccg gcttgccggc
     3121 ctgcataacg gccattgccg agttggtggt acctaggtcg attccgataa ttttagccat
     3181 cgttttattc ctccgttctt ttactcttga ttggggttat cgtcgggtat ttcggctgcg
     3241 atatctttga gtgacgtatc cagctcaacc aactccgtta tacggggcaa ctgttctctg
     3301 aaatggcccg ctgctgcctg gagcgggctg aatgcctcgc cgctgcggac tggtggcgac
     3361 attacaaatg aatgcctggc actgctaaat atcgtgcctc cgatgcgctt ggctttttgc
     3421 cgccatgagg tcttcggtaa atattcttcc atagttggct cctagcaatt agctattgtg
     3481 agtgctaata ctattgtagc atagaaaatt agcactcctg tgtcaagagt gctaattttg
     3541 cttttcaata atatgtccta gcgcatagta gacagtgatt gcagccctgt gccgctggga
     3601 cgctcgatca tgtggtagct cggcaatggc ccgttcgccg agagcctcaa tatgttcccg
     3661 atccaactga acttccaacg gcagcccctc ttcttgccct tttgccaaag ccgcctcgat
     3721 ggccgcgtag gcgtattgag gtgctggcgg taccgggcga cgcatgttta cctcattttg
     3781 acgcggacca tggcgtggcg gatgacttca tcacccaacc ggtagccggc ttgcagctct
     3841 tcgctgacga cctcgttggt gccgtcgccg tcttccatgc tgatggcttc gtgatacttc
     3901 gggtcgaacg gttcgccgac ggtcttaatg cgctccacgc ccaggccgtc cagtgtcttt
     3961 tcgaactgtt tgaccacgcc ttggacgcct ttgacgaagt cattgccttc caggtctttc
     4021 ggtacgtgct tcagggcccg ctcgaaattg tcgataatcg gcaacagatc gcggattact
     4081 ccggatttga ctacattctt cagtcctgcc agttgctcct cgtgccggcg gcggacgttg
     4141 ataacgtcgg cgcgctcgcg ctgcagtgca tcggtcagct cggcaacttg ttgttccagt
     4201 tgctccagct cgtcattatc gtggtgcttt gcaggtttct tggttttatc ggtcatgatt
     4261 cacctcctgc ggcgcttggt ttgggtccgg gtttaaaaat tgcagcataa cttcgccggc
     4321 cttgccgagc gcgttagcaa tggtaactcc tataaagcgg accagcggtc cttgcggcag
     4381 tcgatcctga atagggcgcg gtgcttcgtt gcgtgtcaca gacattacag cacctcctct
     4441 aaggtttttc cggtgtgggc gaccagcgcc atgacgtcgc gataactttg gcgtgtcgga
     4501 ccaagcgtac cgatgtagct gtggtcggag aacggactgc ggaaacggct gatgatcagg
     4561 ctgacgccgg cgctgcggcc gatggggttc tcgctgccga tgaacacgct gagcggctcg
     4621 ttgggagcgg cctcgcgcag ccacggttcg aggttatcga gcagattcgc cacctgctgt
     4681 acctgctggg tgctgctcag gaattccggc tgaccgaaca ggttggacag accgctgata
     4741 tacagctgat tgccgatggt tgccaggcca aggttatgcg tcagctcgac cagcgtatcg
     4801 acggcgttgc ggatggtgcg ttccggcagc ccaccgtggg tcacccgggc ggtcagagca
     4861 cgctcggcgc ggcgctccgg ggcgctgagg gcggtggatt cctgcaggtt attcacataa
     4921 aagcggtagc cgcggtcggt gggaatgcgg ccggcactgg tgtgcggctg ccggatgtaa
     4981 cccaggcgct ccagctcggc catctcggcc cgtatggtcg cgctcgacac gtcgaatacc
     5041 ttggcgagca agctgctgcc gaccggcacg gcgacctcgg cgtactgctc aataatcgcg
     5101 gacaaaattt gcttttgtcg ttctgtcatg gcataaagta tacgctttta gcactcaaaa
     5161 gtccagactg ccagcgatac tgccgcttag cggcccaaga attgctgcgt atcgtgcagg
     5221 agctgttctg caatggctcg ggcttcatca tcgtcgggga catccagacc ttgggacatg
     5281 gcgtgtatgt cacgcgcagt gtgcttgaac gtatcattga ctacgaaccg gtaataatcg
     5341 tgcgccaacg cgacctgcag ctcctggacg gcactctcca gccggcgcct ggtttcgtct
     5401 tcgggcaatt cgccgcgggc tttcatgcgg gtcatccact cgtcaaagct gggcggcacg
     5461 atgaacaaaa acagcgcgtc cggctttacg gcatggatgt tttcgccgcc gacaacctcg
     5521 atctcgttga tggcgatctt gccttccgcg gcggccgccg acagctcgcg gatactgata
     5581 cccgatacct gctggttatg gatgatggcc gcctccagga acatgccggc ttgcagatcg
     5641 gccagcagtt cgtcttcggt acggaaccag tattccctcc cgttctgttc cggcacgcca
     5701 ttgtttatcc ggggcttgcg ggtcgtgtcg ctcacgatgt agtgataatc gccggtcgta
     5761 agcagctcgt tgatgatggt attacggccg ctggagctgg gtccgaccag caacaccagt
     5821 ttggtctttg ccaagatgct ccgggcttcg tcggacacat ggtaatcggc caatacctgg
     5881 cggaattcgt cgatgcgcgt aagctgattc atcaactagt tataacagat tgggtggtaa
     5941 tcataaaggc gaggctggca agtggttacc agctagggta gctggtgctg agcggctgct
     6001 ttcgctgtcg ctgcaactgc ctgcggagct gccgtcggcg gcaactgacc tgcttgcggg
     6061 agcgaagcag cagcgtgctc cttggccggc gccggtggcg tggcggccgg gctggccatg
     6121 gtctgggtca tctctcctgt ttgagtagta gccacgtgcg tacccgcagg ttgagggacg
     6181 tttacggcgt caggggccat gctgttcggt cctgacagat ttgctgctgc cagtgtcgag
     6241 gacgcgccga gctgactgat ggtaaacaag ctgggcttgc ccacaacggc ttcacgatta
     6301 gtcgcttgcg tagccgagtg caccgtaacc acgacaaaca ggatgaagcc aatggttagg
     6361 acggtaatac ggagaatctg ccgggataac tggaacatgc tggtgccttt ctgtttaggt
     6421 cagtggcata agcatagccc gccggcaaag tatccgatat gaaatatttg acaaataatc
     6481 taaaaactta ccagtactgt gagctactgt gattctttat tctggcgcct ggctcgtgct
     6541 tcagtagcgg caattcctac tattgcgcct acgcaaaggg tggctgcatc taccgcgaag
     6601 tggtcgatat tcaacacttc cggagcccgg cttagcatgg ccatcaccgc ttggcccatc
     6661 tctgccagtg cgacgccaca gacacccgcg cgcatagctc ctcgaagtcc ttcccgtata
     6721 gaaggctcat cactgggatc gattctttcg gacatgccgc cattattgaa cggaacgccc
     6781 attaattcaa tggaaccggc gtaactagtc gcgtttgagc atgacggtga aggcgtcggc
     6841 cgggatgtcg accttaccga aacgcttcat gcgcttcttg cctttggcct gcttggccag
     6901 cactttcttt ttgcgcgaca cgtcgccgcc gtacaggccg acggtcacat ctttacggta
     6961 ggcggaaatg tcttcgcggg cgatgaaccg gccgccgatg gctgcctgca gcgccacctg
     7021 gaagttctga cgcggaatga tttccttgag cttctgcacc gtttcgcgcc ccaggcgctc
     7081 tgcctcgctg cggtgcacca tcacacggag cgcactcacc atgtcgccgg ccacgtagaa
     7141 atcgacgcgc accaggtctt ccgcccggta gtcggccagt tcgtagttga agctgccgta
     7201 accgctggtc aggctcttga gctgatcata aaagtcggtc agcaggttgg ccagcggcgc
     7261 ctcgaaactt accactgccc gctggtcgac gtaagacaga ttcttctgca ggccacgctt
     7321 gc
//
LOCUS       DASSFD010000217         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_358606, whole genome shotgun sequence.
ACCESSION   DASSFD010000217 DASSFD010000000
VERSION     DASSFD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358606"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFH39_05265"
     CDS             <1..388
                     /locus_tag="VFH39_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925211.1"
                     /translation="QQDVWIEYQNTGAQPWYDNTSKPAGGYAVHYANTNPINRASQFY
                     DSTTWLNNNRPSRQFAAVYESDGTTLAANQHVAQPGQIVKFSYNYAAPANAAPGTYTE
                     DIQLVREGATDWNFGGWGFFKVTVSP"
     gene            427..1752
                     /locus_tag="VFH39_05270"
     CDS             427..1752
                     /locus_tag="VFH39_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6925212.1"
                     /translation="MRILFLTIDAEENMRGIGSILKSLMRAARAEGHEVGLLTGIPYK
                     HAYEGDSELQAKIEHVHLQHYLLEGRKSFKYMIPGGYRKKNLLKKMLSLSFLDHAFMP
                     VQPELLSGKRTLAYDMDFIVRAPFVYQFIARNKARISRKVVGRIARSYDIDLVFAVSP
                     TVLRSKDLGRKTKLATFVHDIMPLETLETPADNDTPARFARQVETAVKNSDLLMANSK
                     STADKVMRFAPKVDIDVVYGVVSSAKTEIIDSAILQIRNLRPNHYLLFASAVEKRKNL
                     EGLFEAYALAFDHIRMPLVIVGAPGYGFEEIVDKYESLPDHIRENILFTGYASEDDKF
                     TLFKHAHAFVFPSFTEGLGVQNIEAMSYGIPVLSTNVDAIPEVAGDAALLVNNPYDVH
                     EIADALVRICNDEALRKKLIEAGMHKYQNFTFEKFQQRVGHSLNRLGKR"
     gene            1875..>2163
                     /locus_tag="VFH39_05275"
     CDS             1875..>2163
                     /locus_tag="VFH39_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925213.1"
                     /translation="MDETLYFIHQIKNGMTVKTGEYEALTRLFTGQKMFVDTRDISLA
                     PHLMFDGIWEEPLTKLILSALNENSVFLDVGANFGYFGLIAGTKIKNGSLHF"
BASE COUNT          547 a          596 c          557 g          463 t
ORIGIN      
        1 acagcaggac gtctggatcg aataccaaaa tacgggggct cagccgtggt acgacaacac
       61 gagtaagccg gcaggtgggt atgccgtaca ttacgccaat accaacccaa tcaatcgtgc
      121 tagccagttc tacgacagca cgacttggct gaacaataac cgaccctcca gacaatttgc
      181 tgccgtctac gaaagcgatg gcactacgct agctgccaat cagcatgtgg ctcagccagg
      241 gcagatcgtg aagttttcat acaattatgc cgcgcctgca aatgcagctc cggggacata
      301 tacggaagac atacagctcg ttcgtgaagg tgctactgat tggaactttg gcggctgggg
      361 cttcttcaag gttaccgttt cgccctaact ctctgttaca atagagggac gtaaggaaat
      421 tctttgatgc gaatactgtt tttgactatt gatgccgagg agaacatgcg cggcatcggt
      481 tctattctta agagtctgat gcgggccgcc cgcgccgagg gccacgaagt cgggttgctc
      541 accggcatcc cttacaagca cgcctacgaa ggcgattcgg agctgcaggc caagattgag
      601 cacgtgcatt tacagcacta cctgctggaa ggccgcaaaa gctttaagta catgattccg
      661 ggcggctacc gcaagaaaaa cttgctaaaa aagatgctca gcttgagctt cctcgatcac
      721 gccttcatgc ccgtccagcc agagctactc tccggcaagc gcaccctggc ctacgacatg
      781 gacttcattg tccgggcgcc attcgtctac cagttcatag cgcgcaacaa agcacgcatc
      841 agccggaagg tcgtcgggcg catcgcccgg tcgtatgaca tcgacctggt gttcgccgta
      901 tcgccgaccg tgctgcgcag caaagacctg ggccgcaaga ccaagctggc gacgttcgtg
      961 cacgacataa tgccgctcga gacacttgag actccggcag acaacgacac ccctgcccgc
     1021 tttgcacggc aggttgagac ggccgtaaag aactccgacc tgctcatggc caactcgaaa
     1081 agcacagccg ataaggtcat gcgcttcgcc ccgaaagtag atatagatgt cgtgtacggt
     1141 gtagtgtcga gcgccaagac agagattatc gacagcgcta tactgcagat acgcaacctg
     1201 cggcccaacc attacctgct attcgcatct gcggtcgaga agcgcaagaa cctggagggt
     1261 ctgtttgagg cctacgcctt agccttcgac cacatccgta tgccgctggt cattgtgggc
     1321 gcgcccggct atgggttcga ggaaatcgtc gacaagtacg agtccctgcc cgaccacatc
     1381 cgcgagaaca tcctctttac cggctacgcc agcgaggatg acaagttcac cctgttcaag
     1441 cacgcgcatg cctttgtgtt cccgagcttt accgaaggcc tcggcgtcca aaacatagag
     1501 gccatgagct acggcatacc ggtgctcagc accaacgtgg acgccattcc ggaagtcgct
     1561 ggcgatgccg ccttgctcgt gaataatccg tacgatgtcc acgagatcgc cgacgccctg
     1621 gtgcgcatct gcaatgacga agcactacgc aaaaagctca ttgaggctgg catgcacaag
     1681 taccagaact tcaccttcga gaaattccaa cagcgcgtcg gtcattcatt aaacagactg
     1741 ggtaagaggt agggagttta taaatggtaa ggattccggg taggccccaa cgtaacctcc
     1801 gatttaaagc agaagacttc atacgcaatg cgcgtcacaa gtctgcgcct ggtggtgaat
     1861 tatccgctgc taagatggac gagactctgt atttcatcca tcagatcaag aacggtatga
     1921 ccgtaaagac gggtgagtat gaagcactta ctcggctatt cacaggtcaa aaaatgtttg
     1981 tggacactcg agatatctca ttagctcctc atttaatgtt tgatgggata tgggaagagc
     2041 ctctcacaaa actaattctt tccgcgctaa acgaaaactc ggtatttctt gatgtaggag
     2101 ccaactttgg ttatttcgga ctgattgcag gcaccaaaat aaagaatggc agcctccact
     2161 ttt
//
LOCUS       DASSFD010000218         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_497297, whole genome shotgun sequence.
ACCESSION   DASSFD010000218 DASSFD010000000
VERSION     DASSFD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497297"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..474)
                     /locus_tag="VFH39_05280"
     CDS             complement(178..474)
                     /locus_tag="VFH39_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6925214.1"
                     /translation="MQYHNGFDAGKEDQNKLQQVQLENEQVQVQLDSRLQNVSKLVLN
                     DAHKTFTFDMRDVQGQLENCNGTYGDSQAVARADLAHLSCVRSIDITPTATPSK"
     gene            complement(791..>2044)
                     /gene="obgE"
                     /locus_tag="VFH39_05285"
     CDS             complement(791..>2044)
                     /gene="obgE"
                     /locus_tag="VFH39_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6925215.1"
                     /translation="EVTVIAGDGGNGKLSFRHEKFIDKGGPDGGDGGNGGDVVLVASR
                     NETTLAAFRYQKELKATDGEPGGKQRMHGKSAGDLEVSVPVGTVVMDEAGKVLADLTH
                     DEQKAVIAHGGRGGYGNAHFVSSTRQAPKFAEKGEPGDSLQVKLELKMIADVGLVGLP
                     NAGKSTLLSRLSNAKPEIADYPFTTLTPNLGVVDIDTDTSVLIADIPGLIEGANEGKG
                     LGHEFLRHVERTSVLIHLIDAYQPDVPEAYRTIRTELEKHDPQLAKRAEIVALNKIDG
                     LDAEIVDDLLTQLRAATGPKAQLLAVSAQSGQGIRELLYAVKAAVLKARTKQAKQAEK
                     SAVPVLTLGDQSRRWQVRKEGATFVVSGPRIEKFAQRTDFSNEQGLRRLRDIMNKMGV
                     MHELNRRGIKAGATIRIHGAGTFEY"
BASE COUNT          386 a          620 c          549 g          489 t
ORIGIN      
        1 caaaacatcg ggcgacagcc gtatgagcac gttcaccggc tcaccaagcg gcttcaagtc
       61 caagtcggtc cgctgcccgg cgaactgcat aatgcggcac tctcgccact tcactccggt
      121 cgtctcgagg tcgtagaaga aaaaactggc agccataggt agctaccagt ttatcattta
      181 tttggacggt gtagcggtcg gagttatgtc gatactacgt acacaactta ggtgagcgag
      241 atccgctctg gcaaccgctt ggctatcgcc gtacgtgccg ttgcaattct ccagctggcc
      301 ctggacatca cgcatatcaa aagtaaaagt cttgtgagca tcgtttagca caagcttgct
      361 cacgttctgt aaacggctat caagctggac ttgtacttgt tcgttctcta actgcacttg
      421 ttgcagctta ttctggtcct ctttgccagc atcgaaccca ttgtgatatt gcatgaaacc
      481 ctgtatgagg gatccgacag catagccaac gaaatatgtt gcccatgttg tcattaccga
      541 ccctctgtcg tcacgcagct ccgtgaaatt gatacgcatt acaaaagcgc ctccttgacg
      601 ctaaaagaat taaacatttc gccttagtct gcgagtgctt attcctagcg gagacgctgt
      661 attgcttgcg tacgctacgg cttgggcata gctgtttcgg tagccgccaa aatcactggc
      721 tgtttacttg cggctagaac cggttcttcc cgattccgta actacattat accacattgt
      781 caatatattg ctaatattcg aacgtgccgg caccgtggat gcggatggtc gctccggcct
      841 taatgccccg gcggttgagc tcgtgcatga cacccatctt gttcatgatg tcgcgcaggc
      901 ggcgcaaccc ctgctcatta ctgaagtcgg tacgttgggc gaacttctcg atgcgcggcc
      961 cgctgaccac gaaggtagcg ccttccttgc gcacctgcca gcgccgcgac tgatcgccca
     1021 gcgtcagtac cggcacggca gacttctcgg cttgtttggc ctgcttggta cgagccttga
     1081 gaaccgctgc cttgacggcg tacagcagct cgcggatgcc ttggccggac tgagccgaca
     1141 ctgccagcag ctgcgctttc gggccagtcg ccgcgcgcag ttgcgtcagc agatcatcga
     1201 cgatctcggc atcgagcccg tcgattttgt tgagcgccac gatctccgcc cgttttgcca
     1261 gctgcggatc gtgtttttcg agctcggtgc ggatggtcct gtacgcttcg ggcacgtccg
     1321 gttggtacgc atcaatgaga tgtatgagca cggaggtgcg ctctacatgg cgcaggaact
     1381 cgtggcccag acccttgcct tcgttggcgc cctctatgag cccggggatg tccgcgataa
     1441 gtaccgaagt gtcggtgtcg atgtcaacca cgccgaggtt cggtgtcagc gtggtgaacg
     1501 ggtaatcggc gatctccggc ttggcgttgc tgagccggct gagcagcgtc gacttaccgg
     1561 cattcggcag gccgaccagg ccaacatcag cgatcatctt cagctcaagc ttaacttgta
     1621 aactatcgcc gggttcgccc ttctcggcga atttcggcgc ttgccgggtg ctgctcacga
     1681 agtgggcgtt gccgtagcca ccccggccac cgtgggcaat aaccgccttt tgctcgtcgt
     1741 gggtcaggtc ggccagcacc ttgcctgctt cgtccatcac gaccgtgccg actggtaccg
     1801 acacctccag gtcgccagca ctcttgccat gcatgcgctg tttgccgccc ggttcgccat
     1861 ccgtcgcttt caattccttc tggtaccgga aggcggccag cgtagtctcg ttacggctgg
     1921 cgaccagcac gacgtcaccg ccattaccgc catcgcctcc gtccgggcca cctttgtcga
     1981 tgaacttctc gtgccggaaa ctcaacttgc cattgccgcc gtcgccagca atgactgtaa
     2041 cctc
//
LOCUS       DASSFD010000219         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_1131678, whole genome shotgun sequence.
ACCESSION   DASSFD010000219 DASSFD010000000
VERSION     DASSFD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131678"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFH39_05290"
     CDS             complement(<1..715)
                     /locus_tag="VFH39_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET6925216.1"
                     /translation="MRKKASWSDKLDAFFSITARGSTLGREVRGGFVTFFAMAYIVVL
                     NPIILASGVDKFGHHLSVAQLTTATALIAAIMTALMGLGANLPLAIAAGLGLNGVLAF
                     QIAPTMSWPDAMGLVVLEGILICLLVVTGLRRAIMDAIPLALKQAISVGIGLFIAFIG
                     LVDSGFVTIPKSLATPVQLGATGQLGGWPVVIFCFGLLLTIVLLAKKVRGAILLGIVA
                     STVLAVVVNAIAKVANWGLV"
     gene            complement(891..1574)
                     /locus_tag="VFH39_05295"
     CDS             complement(891..1574)
                     /locus_tag="VFH39_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6925217.1"
                     /translation="MTRYRQPPSIRRLFMRGQPLHVQKGEVIIGNDMMPDGVYFLDTG
                     YVKIYSISDEGHEYLHIIYGHGDLFPLTWAYMDSQPSQLYYETISDCTLWRVSRYWFT
                     QMARTNAAISYDLGLQLAYQFRVYSDRIDNLEYKKASDRVIYRLLFLGSRFGTKQDDS
                     LTIDAPITHEHFANSINLARESVSRELEKLEREGLLYRSDNRLVLTDAPALAARLSKP
                     IDHRDWSLL"
     gene            1715..>2030
                     /locus_tag="VFH39_05300"
     CDS             1715..>2030
                     /locus_tag="VFH39_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET6925218.1"
                     /translation="MANLIVLAFENENGAQQTLMEVEELQKQNLIHVEDAATAVRGAG
                     GKVKVKQAHNLAGSGALGGAFWGMLFGLLFFVPFLGLAVGAATGALFGKAADYGINDD
                     FIR"
BASE COUNT          464 a          573 c          587 g          406 t
ORIGIN      
        1 tcaccaggcc ccagttcgca accttggcga tagcgttcac gacgaccgcc agaacggtcg
       61 aagccacgat accgagtagg atggcgcccc ggactttttt ggccagaagt acgatagtca
      121 gcagtagtcc gaagcagaaa atgaccacgg gccagccacc cagctgcccg gtcgcgccga
      181 gctgcaccgg ggtggccagg ctcttcggaa tcgtcacgaa gccggagtcg accaagccga
      241 tgaaggcgat gaacaaaccg atgccgaccg atatcgcctg tttgagcgcc agcggaatag
      301 catccatgat ggcgcgccgc agaccggtga ccaccagcag acagatcaaa ataccttcta
      361 agacaaccag gcccatcgcg tccggccagc tcatggtcgg ggcgatctgg aacgccagca
      421 cgccgttcag gcctaagcct gcagcgatag ccagcggcaa gttggcaccg agccccatga
      481 gggcggtcat gatggccgcg atgagcgccg tcgccgtcgt cagctgcgcc acgctcaggt
      541 gatgaccgaa cttatccacg ccgctggcga gaataatcgg gttcagtacc acgatgtacg
      601 ccatggcgaa gaacgtgacg aaaccgccgc gcacctcccg gcccagtgtg gaccctctgg
      661 cagtgatact aaaaaaggca tccagcttgt cgctccagga tgcctttttg cgcactgacc
      721 ccttaaattt aataggtttg ggtagtgttg ttgctctcat agcagcattg taatgctcat
      781 gcaagcggaa cgccaagcgt aataaataca accgttttac cgctgtatgc gcacgctcgc
      841 aaaggtgtta aatgtcttat gaaaagtact gtttcgctgt gcaaaaatac tcacagcaag
      901 gaccagtcac gatgatcgat cggcttactg agccgcgctg ccagagccgg cgcgtcggtc
      961 agtaccagcc ggttatcgga gcggtacagc aggccttcac gttccagctt ctcgagctcc
     1021 cggctgacac tttcccgggc gagattgatc gagttggcga agtgctcatg ggtaatcggc
     1081 gcatcgatag tcaggctgtc atcttgtttg gtgccgaagc ggctgcccag aaacaataag
     1141 cggtaaataa cccggtcgga tgcctttttg tattccaggt tatcgatccg gtcggaatat
     1201 acccggaact ggtacgccaa ctgcaagccc agatcgtagc tgatggctgc gttggtccgc
     1261 gccatctgcg tgaaccaata acgcgaaaca cgccacagcg tacagtccga aatcgtctcg
     1321 taatagagct gggacggttg cgaatccatg taagcccacg tcagggggaa caggtcgccg
     1381 tggccgtaga taatatgcag gtattcatgg ccctcatcac ttatggaata gatttttaca
     1441 tatccggtat cgagaaagta caccccgtcc ggcatcatgt cgttgccgat gatgacctcc
     1501 cctttctgga catgaagcgg ttgcccccgc atgaacagac gtcggatgct cggtggctgc
     1561 cggtagcgtg tcataggctg tcccccctca tatggtcgct gctcctggct cgatccgctg
     1621 taagatcggt gactcctgac cggtgattgc agtcactgcg tctcagttca ggcaatccca
     1681 tactcgaata cgtaatagca accggggggt aactatggcg aatcttatag tattggcatt
     1741 cgaaaacgaa aacggcgcgc aacagacgct catggaagtg gaagagctgc agaagcagaa
     1801 cctcatccat gtggaggacg ccgccacggc cgtccggggc gccggcggaa aagtcaaagt
     1861 aaaacaggcg cataacctgg cgggatccgg tgcgctgggc ggcgcgttct ggggcatgct
     1921 gttcggactg ctgttcttcg tgccattcct gggcctggcg gtgggggcgg cgactggcgc
     1981 gctgttcggt aaagcggccg attacgggat aaacgacgac ttcattaggg
//
LOCUS       DASSFD010000220         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U996 k141_498011, whole genome shotgun sequence.
ACCESSION   DASSFD010000220 DASSFD010000000
VERSION     DASSFD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963163
            Sequence Read Archive: SRR25158412
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:52:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,062
            CDSs (total)                      :: 1,025
            Genes (coding)                    :: 1,023
            CDSs (with protein)               :: 1,023
            Genes (RNA)                       :: 37
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498011"
                     /isolate="SMAG_U996"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.10888889 N 114.9202778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFH39_05305"
     CDS             <1..711
                     /locus_tag="VFH39_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6925219.1"
                     /translation="TPTVVWLLGVLNPLFSRITTAWHQRFPLEVHSGLYEKSDLIELV
                     QQQRLQADNRVPAEVLELVNRALHFDTYLIRDIVRPRKQLRTLKASEQVGPVLLDELH
                     ATGQTDFPVCEKSADKIIGTLHVPTLDEAKQGGTVADYANRKLAFLHESDSLATALHA
                     FYTTKQRLFVVVNGFDEYVGIVTLEDLMHVLIGDVVPSDFDAYFDRQAVAERHTSHHQ
                     SPEPDIEIPAETAGTVVE"
     gene            852..>2090
                     /gene="aspS"
                     /locus_tag="VFH39_05310"
     CDS             852..>2090
                     /gene="aspS"
                     /locus_tag="VFH39_05310"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6925220.1"
                     /translation="MRTYVADTPQKVGEAITVKGWVNSRRDHGGLIFIDIRDHTGIAQ
                     LNIRPEQSEAFTIGESLRDEFVIAATGTVVEREEGLKNPNIATGGIEIKVDKLNILNR
                     SEALPIQISGDAMANEDLRLKYRYLDLRRPKMQQRLQQRARYYKVMRDYMEAHDFTEV
                     TTPILANSSPEGARDFLVPSRVHPGKFYALPQAPQQFKQLLMVGGVPRYYQLATCFRD
                     EDPRADRLYGDFYQLDLEMSFVEDGEEVRSTMEPLIKQLVTEFAGKKLLSDDIPRIPF
                     TEAMERYGTDKPDLRFGMELVELTDELRETEFGVFKNAEVVKAICVKGAADLSRSQID
                     KFTDIAKSEGAGGLAYLTYGGGEVQSPIAKFLKPEELQAIKAKSGAADGDIVFFGADR
                     RSVVNKVLGRLRNEFADHFNL"
BASE COUNT          490 a          644 c          578 g          378 t
ORIGIN      
        1 acacctacag tcgtctggct gctgggtgtg ctgaaccccc tattctctcg tattaccacc
       61 gcctggcacc agcgctttcc gctcgaggtg cacagcgggc tgtatgaaaa atccgacctc
      121 atcgagctgg tccagcagca gcgcctgcag gccgacaacc gcgtaccggc tgaggtcctg
      181 gagctggtaa accgcgccct gcacttcgac acgtacctca tccgcgacat cgtccggccg
      241 cgcaagcaat tgcgtaccct taaggccagc gagcaagtgg ggccggtact gctcgacgag
      301 ctgcacgcta ccggccagac tgatttcccg gtgtgcgaaa agtcggcgga caagatcatc
      361 ggcacgctgc atgtaccgac gcttgacgaa gctaaacagg gcggtaccgt cgctgactac
      421 gccaaccgca agctggcctt tttgcacgag agcgattcac tagcaaccgc gctgcatgcg
      481 ttctatacca ccaagcagcg actgttcgtg gtggtcaacg gcttcgacga gtatgtcggc
      541 attgtgaccc tcgaggatct gatgcacgtg ctcatcggcg atgtcgtacc cagcgatttc
      601 gatgcctact tcgaccgcca ggcggttgcc gagcggcaca ccagccacca ccagtcaccg
      661 gagccggata tagaaatccc tgccgaaaca gccggaacag tggtagaata gttcccgatg
      721 actgattaca cggtaaagtc caatacgaat cccggggtct gcagctgctg agaagctggc
      781 agcggctgtt tcaagcgacc cataaagagg ctttccaaac cgttaatcta acccaataag
      841 aggtatctac catgcgaaca tacgtcgcag acactcccca aaaagtcggt gaagcaatca
      901 cggtcaaagg ctgggtgaac agccggcgcg accacggcgg cttgattttc atcgacatcc
      961 gcgaccatac cggcatcgcc cagctcaaca tccgaccgga gcagagcgaa gcgtttacca
     1021 tcggcgaatc attgcgcgat gaattcgtca tcgccgccac cggcacggtg gtcgagcgcg
     1081 aagagggact caagaacccc aacatcgcta ccggcggcat cgaaataaaa gttgacaagt
     1141 taaatatcct gaaccgctcg gaagctttac cgattcagat ttccggcgat gccatggcta
     1201 acgaagacct ccggctcaag taccgctacc tcgacctgcg ccgccccaag atgcagcagc
     1261 gtttgcaaca gcgtgcccgt tactacaagg taatgcgtga ctacatggaa gcccacgact
     1321 tcaccgaagt caccacgccc attttggcca acagcagccc cgaaggcgcc cgcgactttt
     1381 tggttcccag ccgcgtgcat cccggcaagt tctacgcgtt gccgcaggcc ccgcagcagt
     1441 ttaagcagct gctgatggtc ggtggtgtgc cgcgttacta ccagttggcg acctgtttcc
     1501 gggacgaaga cccacgggcc gatcggctgt acggggactt ctaccagctg gatctcgaga
     1561 tgtcattcgt cgaagacggc gaagaagtcc gcagcactat ggagccgctc atcaaacaac
     1621 tggtaactga attcgcgggc aaaaaattgc tgagcgacga catcccgcgt atcccgttca
     1681 ccgaagccat ggagcgctac ggtaccgaca agccggacct gcgcttcggc atggaactgg
     1741 tcgagttgac cgatgaatta cgcgagaccg agttcggcgt attcaagaac gccgaggtcg
     1801 tcaaagccat ctgcgtgaaa ggcgccgcgg acctaagccg ctcccaaatc gacaaattca
     1861 ccgacatcgc gaagagtgaa ggcgccggcg gtctggcgta ccttacgtac ggcggcggtg
     1921 aggtacaatc gccgatcgcc aaattcctga agccggaaga gcttcaggca atcaaagcca
     1981 agagcggggc cgcggacgga gacatcgtct tcttcggggc ggaccgacgc agcgtggtca
     2041 acaaagtact cgggcgcctg cgcaacgagt tcgccgacca ctttaacctc
//
